An automatic control method for pathological tissue staining and a staining machine system

Abstract

The application belongs to the technical field of pathological tissue processing, and particularly relates to a pathological tissue staining automatic control method and a staining machine system. The method provided by the application detects and calculates the staining quality after each staining, adjusts the staining processing time of hematoxylin and eosin according to the staining quality, and replaces the staining solution if the staining processing time of hematoxylin and eosin exceeds the preset maximum value. The method provided by the application can effectively prolong the service life of the staining reagent, and can also ensure the uniformity and stability of pathological section staining.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of pathological tissue processing, and particularly relates to a pathological tissue staining automatic control method and a staining machine system. BACKGROUND

[0002] With the development of pathological technology, fully-automatic pathological tissue staining machines are widely used. However, with the change of factors such as the number of times of use of staining solution and time, the quality of pathological tissue staining is nonlinearly reduced, which leads to the need to frequently replace the staining agent, increases the staining cost, and cannot guarantee the stability of the staining quality. In the prior art, the control mode is too simple, and the stability of the staining quality is not good, and the frequent replacement of the staining solution also leads to the increase of the cost. SUMMARY

[0003] In order to solve the above problems, a pathological tissue staining automatic control method is provided. The application prolongs the service life of the staining solution by adding a staining solution stabilizer in the staining step, and simultaneously obtains the color change of the pathological tissue on a certain number of slides through a color sensor, automatically balances the staining time of each key staining step according to the color change trend, thereby maximally prolonging the service life of the staining reagent, and also ensuring the uniformity and stability of pathological section staining.

[0004] According to one aspect of the application, a pathological tissue staining automatic control method is provided, comprising the following steps:

[0005] S1: replacing the staining solution;

[0006] S2: sequentially performing xylene dewaxing treatment, hematoxylin staining treatment, blueing solution treatment, and eosin staining treatment on the pathological tissue;

[0007] S3: performing color data scanning on the pathological tissue after the eosin staining treatment is completed, and calculating the staining quality;

[0008] S4: judging whether the staining quality has a continuous downward trend:

[0009] 1) if there is no continuous downward trend, returning to S2, and performing the hematoxylin staining treatment and the eosin staining treatment according to the last hematoxylin staining treatment time T he and the last eosin staining treatment time T eo ;

[0010] 2) if there is a continuous downward trend, recalculating the hematoxylin staining treatment time T he and the eosin staining treatment time T eo , and judging the recalculated hematoxylin staining treatment time T he and the eosin staining treatment time Teo whether the preset maximum value is exceeded:

[0011] 2.1) if the recalculated hematoxylin staining processing time T he and eosin staining processing time T eo exceeds the preset maximum value, return to S1;

[0012] 2.2) if the recalculated hematoxylin staining processing time T he and eosin staining processing time T eo do not exceed the preset maximum value, return to S2.

[0013] Optionally, in the step 2):

[0014] the hematoxylin staining processing time T he is obtained based on the hematoxylin standard staining time T b , the amount of slides processed by hematoxylin X he after the last change of staining solution, the number of days D he hematoxylin has been used, and ΔQ adjustment calculation;

[0015] the eosin staining processing time T eo is obtained based on the eosin standard staining time T o , the amount of slides processed by eosin X eo after the last change of staining solution, the number of days D eo eosin has been used, and ΔQ adjustment calculation;

[0016] wherein, ΔQ = Q best - Q bad , Q best is the staining quality after the last adjustment of staining time, and Q bad is the staining quality of this time.

[0017] Optionally, the calculation method of the hematoxylin processing time T he and the eosin processing time T eo is:

[0018] T he = p1*X he ^4 + p2*X he ^3 + p3*X he ^2 + p4*X he + p5

[0019] wherein, X he is the amount of slides processed by hematoxylin after the change of staining solution, p1 = 1.122, p2 = -9.557, p3 = 14.85, p4 = 120.4, p5 = T b + ΔQ*300 + D he *5, Tb T is the hematoxylin standard staining time, D he T is the hematoxylin used days;

[0020] T eo = p1*X eo + p2

[0021] wherein, X eo is the amount of eosin treated slides after changing the staining solution, p1 = 4.472, p2 = T o + AQ*10 + D eo , T o is the eosin standard staining time, D eo is the eosin used days.

[0022] Optionally, the hematoxylin standard staining time is 2-4 min, and the eosin standard staining time is 8-12 s.

[0023] Preferably, the preset maximum value of the hematoxylin staining time is 10-14 min, and the preset maximum value of the eosin staining time is 1.5-2.5 min.

[0024] Optionally, in the step S2, the xylene dewaxing treatment time T xf The counter-blue solution treatment time is 50-70 s according to the ambient temperature adjustment.

[0025] T xf = -18*T ev + 720, wherein T ev is the ambient temperature.

[0026] Optionally, in the step S3, after the staining is completed, at least 8 fixed sampling points are selected on the slide, color data is scanned and converted into the HLS color space, according to the brightness L, 2-4 groups of data with the minimum L value are selected to calculate the staining quality.

[0027] Optionally, the calculation method of the staining quality is: Q = (1-L curr ) / (1-L best );

[0028] In the formula, L is the average value of L calculated from 2-4 groups of data with the minimum L value in the selected sampling points, wherein L best is obtained according to the staining quality after the first staining according to the standard staining time after changing the staining solution, wherein L curr is obtained according to the staining quality after the present staining.

[0029] Optionally, the S2 xylene dewaxing treatment is followed by a stable liquid immersion treatment before the hematoxylin staining treatment, and the stable liquid immersion treatment time is 5-10 minutes. A stable liquid is added before the staining solution to reduce the impact of the water carried out on the staining solution, stabilize the properties of the staining solution, and increase the number of stained sections.

[0030] Optionally, the stable liquid comprises a buffer solution, a polyhydric alcohol, and a microbial inhibitor, wherein the buffer solution is a citric acid solution or a hydrochloric acid solution, and the polyhydric alcohol is one or more of a diol, a triol, or a polymer thereof.

[0031] Preferably, the buffer solution is one of a disodium hydrogen phosphate-citric acid buffer solution, a phthalic acid-hydrochloric acid buffer solution, and a citric acid-sodium hydroxide-hydrochloric acid buffer solution.

[0032] The polyhydric alcohol is selected from one or more of propylene glycol, glycerol, dipropylene glycol, and polypropylene glycol.

[0033] The microbial inhibitor is selected from one or more of benzalkonium chloride, benzoic acid and its derivatives, and isothiazolinyl ketone bacteriostatic agents.

[0034] The staining solution stabilizing liquid is mainly composed of a buffer solution, a polyhydric alcohol, and a small amount of auxiliary materials. The solvent of the stabilizing liquid has similar properties to the staining solution, so that the surface tension of the stabilizing liquid is consistent with that of the staining solution, and the pH is similar, which has a certain buffering effect on the pH and ionic strength of the staining solution, thereby avoiding the adverse effects caused by the carry-out of the solution. The staining solution stabilizing liquid is mainly used before the hematoxylin staining solution step of the pathological tissue section to buffer the effects of water washing on the pH and ionic strength of the staining solution, and the service life of the HE staining reagent can be greatly increased by using the stabilizing liquid.

[0035] According to another aspect of the present application, a staining machine system is provided, which comprises any of the above-mentioned automatic control methods for pathological tissue staining.

[0036] The beneficial effects of the present application include but are not limited to:

[0037] 1. The automatic control method for pathological tissue staining provided by the present application effectively prolongs the service life of the pathological section staining solution and reduces the number of reagent replacements.

[0038] 2. The automatic control method for pathological tissue staining provided by the present application effectively ensures the consistency of the staining effect of pathological tissue sections and improves the quality of pathological diagnosis of reading sections.

[0039] 3. The automatic control method for pathological tissue staining provided by the present application has small calculation amount and low cost, does not require complex image processing, and has fast response speed. BRIEF DESCRIPTION OF DRAWINGS

[0040] The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiments of the application and together with the description serve to explain the application. In the drawings:

[0041] Figure 1 The control flow chart of the pathological tissue staining automatic control method involved in the embodiments of the application;

[0042] Figure 2 The slide color scanning principle involved in the embodiments of the application;

[0043] Figure 3 The different batch staining results involved in the embodiments of the application: A, the 5th batch; B, the 15th batch; C, the 25th batch; D, the 35th batch; and E, the 45th batch.

[0044] The label name and label list:

[0045] ① color sensor light sensitive area; ② interference light shielding area; ③ slide staining area; ④ white light source; and ⑤ slide. DETAILED DESCRIPTION

[0046] The application will be described in detail below with reference to the embodiments, but the application is not limited to these embodiments.

[0047] Unless otherwise specified, the raw materials and reagents in the embodiments of the application are purchased through commercial channels.

[0048] With the development of pathological technology, the fully-automatic pathological tissue staining machine is widely used, but with the change of the use times and time of the staining solution and other influencing factors, the pathological tissue staining quality has a non-linear decline problem. The application provides a pathological tissue staining automatic balance control method. The method prolongs the service life of the staining solution by adding a staining solution stabilizing solution in the staining step, and automatically balances the staining time of each key staining step by simultaneously obtaining the color change of the pathological tissue on a certain number of slides, so as to maximize the service life of the staining reagent, and also ensure the uniformity and stability of the pathological section staining.

[0049] The application provides a staining time automatic adjustment mechanism. The pathological tissue staining machine automatically stains according to the standard processing time according to the reagent replacement condition, calculates the RGB average of a certain number of slide staining areas after white balance by scanning the completed slides, judges whether the staining quality Q has a downward trend, continues to run according to the current parameters if there is no downward trend, and automatically adjusts the optimal working time of each corresponding step according to the hematoxylin and eosin processing time and the number of days of the reagent used if the staining quality Q value has a downward trend. Meanwhile, the xylene dewaxing time is automatically adjusted according to the change of the environmental temperature.

[0050] Figure 1is the whole control method flow chart of the present application, and the automatic control method of dyeing time is as follows.

[0051] When the pathological tissue dyeing equipment starts to dye after replacing new reagents, the pathological tissue dyeing process reaches the xylene dewaxing process, and the dewaxing time T is automatically adjusted according to the environmental temperature xf , wherein T xf is calculated as follows:

[0052] T xf = -18*T ev + 720, wherein T ev is the environmental temperature in Celsius, and T xf is in seconds.

[0053] When the process reaches the stable liquid, the immersion dyeing time T h is 5-10 min. The dyeing liquid stabilizing liquid is used before the hematoxylin dyeing liquid step of the pathological tissue section to buffer the influence of water washing on the pH and ionic strength of the dyeing liquid, improve the service life of the dyeing liquid, and at the same time, improve the stability of the quality of each dyeing.

[0054] The dyeing liquid stabilizing liquid is composed of the following components: 75-100 parts of a buffer, 0-35 parts of a polyhydroxy alcohol, and an auxiliary material. The auxiliary material is one or more of a microbial inhibitor or a bactericide, wherein the concentration of the auxiliary material is 0.0%-0.1%, and the preferred concentration is 0.02-0.04%. The buffer is a solution containing citric acid or hydrochloric acid, and the pH buffering range is 2.2-3.8. The polyhydroxy alcohol is mainly diol or triol and its polymer, which is one or more of propylene glycol, glycerol, dipropylene glycol, and polypropylene glycol. The microbial inhibitor or bactericide is one or more of benzalkonium chloride, benzoic acid and its derivatives, and isothiazolinyl ketone preservative bacteriostatic agent. Specifically, the buffer is one of disodium hydrogen phosphate-citric acid buffer, phthalic acid-hydrochloric acid buffer, and citric acid-sodium hydroxide-hydrochloric acid buffer.

[0055] When the process reaches hematoxylin, the hematoxylin dyeing time is adjusted according to the color change of the last batch because the hematoxylin will become lighter after being diluted, and the calculation method is as follows:

[0056] T he = p1*X he ^4 + p2*X he ^3 + p3*X he ^2 + p4*X he + p5

[0057] , wherein X heThe amounts of hematoxylin-treated slides after the staining solution was changed are: p1 = 1.122, p2 = -9.557, p3 = 14.85, p4 = 120.4, p5 = T. b +ΔQ*300+D he *5,T b D is the standard staining time for hematoxylin. he T represents the number of days since hematoxylin was used. he The unit is seconds.

[0058] Where, ΔQ=Q best -Q bad Q best To assess the staining quality after the last adjustment of staining time, Q bad To determine the staining quality for this staining, ΔQ can be understood as the difference between the staining quality Q when the staining time was adjusted last time and the staining quality Q for this staining.

[0059] The following is a further explanation of ΔQ, which measures the degree of decline in staining quality, where Q... best To determine the staining quality after the last adjustment of staining time, the staining quality after the first staining after each adjustment should meet the requirements and, theoretically, should be the best among all subsequent stainings. Therefore, Q is used as the basis for this determination. best As a baseline, it is used to measure the degree of decline in staining quality. Each time the staining time needs to be recalculated, that is, ΔQ needs to be calculated each time, it indicates that the staining quality is poor, and the staining quality Q shows a continuous downward trend, therefore it is denoted as Q. bad Furthermore, by calculating ΔQ, the staining time can be increased accordingly. The larger ΔQ is, the more the staining time is increased, thus effectively ensuring the uniformity and stability of the staining quality.

[0060] When the processing flow reaches the blue return process, the blue return time T b It lasts for 50 to 70 seconds.

[0061] When the processing reaches eosin staining, the eosin staining solution is easily diluted, affecting the staining effect on proteins in the cytoplasm. Therefore, the eosin staining time needs to be adjusted based on the color change of the previous batch. The calculation method is as follows:

[0062] T eo =p1*X eo +p2

[0063] Among them, X eo The amount of eosin-treated slides after changing the staining solution is p1 = 4.472, p2 = T. o +ΔQ*10+D eo T o D is the standard staining time for eosin. eo For the number of days that Yihong has used, Teo The unit is seconds, where ΔQ = Q best -Q bad Q best To assess the staining quality after the last adjustment of staining time, Q bad Regarding the staining quality, ΔQ has already been explained in the formula for calculating the staining time of hematoxylin above, and will not be repeated here.

[0064] After staining is complete, a certain number of slides are scanned by a scanning mechanism, and the number of slides scanned does not change randomly. The scanning principle is as follows: Figure 2 As shown, ① is the photosensitive area of ​​the color sensor, ② is the area blocked by interfering light, ③ is the stained area of ​​the slide, ④ is the white light source, and ⑤ is the front view of the slide. A white parallel beam of light illuminates the slide, and the color sensor reads the original RGB color values ​​on the other side of the slide. As shown in the front view of the slide in ⑤, the slide is divided into 8 regions. The motion mechanism moves in the Y and Z directions and scans the color values ​​of 11 regions from a to k. After scanning, 11 sets of white-balanced RGB values ​​are calculated according to the scaling factor. Then, color space conversion is performed based on the RGB values ​​to calculate 11 sets of HLS values. The calculation method is as follows:

[0065]

[0066]

[0067]

[0068] Where max represents the maximum value of RGB and min represents the minimum value of RGB.

[0069] Sort the data by L value from the 11 HLS groups, and calculate the mean of the 3 groups with the smallest L value. when satisfy Under certain conditions (where H represents hue, staining must meet certain hue conditions to be considered acceptable; for example, due to the use of HE staining, the stained pathological tissue will appear purple. Generally, the average hue value of HE-stained pathological tissue is within this range and is unlikely to appear outside this range. If an occasional hue value appears outside this range, the staining result should be discarded), according to... The staining quality Q is calculated using the following formula:

[0070] Q = (1-L) curr ) / (1-L best In the formula, L is the average value of L calculated from the three sets of data with the smallest L values ​​among the selected sampling points, where L... best The value of L was calculated based on the staining quality after the first staining at the standard staining time following a change of dye solution.curr According to the dyeing quality after this dyeing is calculated.

[0071] Wherein L represents the brightness, the greater the brightness value represents the closer to white, then the dyeing quality is poor, and the smaller the L value, the better the dyeing quality can be considered. Since the first day of dyeing (when the above formula is calculated, the use time of the reagent in the first day is taken as 0), the dyeing quality should be the best when the dyeing agent is replaced for the first time according to the standard dyeing time (when the above formula is calculated, it can be known that the first dyeing after the dyeing solution is replaced is dyed by using the standard dyeing time), therefore the dyeing quality Q measured for the first time after the dyeing agent is replaced is taken as the best dyeing quality Q best At this time, the L obtained after detection and calculation is L best And with the progress of dyeing, the dyeing quality tends to gradually deteriorate, so the L after this dyeing and detection is taken as L curr Used to measure the degree of decline in dyeing quality.

[0072] When the dyeing quality Q remains relatively stable, the last dyeing time is maintained, and the dyeing is restarted; when the dyeing quality Q shows a trend of decline, the dyeing time of hematoxylin and eosin needs to be recalculated and adjusted, if the recalculated dyeing time is adjusted to the predetermined maximum adjustment time threshold, then the new reagent needs to be replaced, otherwise the next round of dyeing process is carried out according to the recalculated dyeing time.

[0073] It can be seen that the above pathological tissue dyeing automatic control method provided by the application mainly contains two layers of circulation process, the outermost circulation process is: when the recalculated dyeing time exceeds the preset maximum value, it is no longer adjusted by increasing the dyeing time, but directly replaces the dyeing agent, here the dyeing agent can be replaced with hematoxylin and eosin reagent, or according to the need, which kind of dyeing reagent whose recalculated dyeing time exceeds the preset maximum acceptable dyeing time is replaced. In actual dyeing process, in order to ensure the uniformity and stability of dyeing quality, the general operation habit is that once either hematoxylin or eosin exceeds the preset maximum acceptable dyeing time, all dyeing reagents are replaced, therefore the first batch of dyeing quality after the dyeing agent is replaced should be the best, and the brightness value L measured at this time after dyeing is taken as L best , so as to be used as the basis for calculating all subsequent dyeing qualities until the next time the dyeing agent is replaced, that is, taken as the denominator part of Q = (1-L curr ) / (1-L best ) in the dyeing quality calculation formula, since the smaller the L value represents the better the dyeing quality, therefore the transformation is carried out by 1-L here, so that the greater the Q represents the better the dyeing quality.

[0074] In the internal circulation process, how to judge whether the dyeing time needs to be adjusted according to the dyeing quality of each time, and how to realize the control of the dyeing quality of each time through the adjustment of the dyeing time to achieve uniform and stable, so as to realize the full use of the dyeing agent, and dynamically adjust the dyeing time according to the dyeing quality after each dyeing to realize the uniformity and stability of the dyeing quality after each dyeing.

[0075] Among them, the dyeing time does not need to be adjusted every time, but needs to be judged according to whether the dyeing quality has a significant continuous downward trend in the process of continuous dyeing of multiple batches. When the dyeing quality shows a continuous downward trend, the dyeing time is recalculated. If the dyeing quality does not continuously decrease, it can be considered that the dyeing quality is relatively stable, and the dyeing time of the last time can be maintained to continue dyeing. Because the dyeing agent will inevitably lead to the dyeing quality of the pathological tissue getting worse with the increase of the use time and frequency, in the actual dyeing process, the dyeing quality of the first ten batches of pathological tissues is good, so the dyeing quality of the first ten batches after changing the dyeing liquid does not change much, and the dyeing time basically does not need to be adjusted. Specifically, in the actual dyeing process, each rack contains 20 slides, so the dyeing quality of the first ten racks after changing the dyeing liquid is very stable, and the dyeing time basically does not need to be adjusted. With the use of the reagent, the frequency of adjusting the dyeing time begins to increase, and each time the dyeing time is adjusted, the time required for dyeing also increases with the use of the reagent and the increase of the time, until the recalculated dyeing time exceeds the maximum acceptable dyeing time, then the dyeing agent is replaced.

[0076] In addition, the condition for judging whether the dyeing time needs to be recalculated is to judge whether the dyeing quality presents a continuous downward trend. Because there are some differences between pathological tissues, such as tissues with high fat content, the brightness L value of the dyeing is relatively large compared with other tissues, and the calculated value of the dyeing quality is relatively poor. More importantly, even if the pathological tissues of similar types, there are often some differences in the dyeing quality, so if the way of judging whether the dyeing time needs to be recalculated only according to the decrease of the dyeing quality of the previous and next two times is not accurate, so here the change trend of the dyeing quality in multiple batches is detected, if the dyeing quality presents a significant continuous downward trend, it means that the dyeing time needs to be recalculated to ensure that the dyeing quality of each batch of pathological tissues is uniform and stable.

[0077] For judging whether there is a continuous downward trend, the specific operator can set according to different dyeing tissues or the control requirements of dyeing quality, for example, according to the specific needs to determine the dyeing quality decline value, and the number of continuous decline times to determine whether the dyeing quality has continuously declined. Those skilled in the art can also select other appropriate ways to judge the continuous decline of dyeing quality according to needs.

[0078] As shown in Figure 3 The dyeing method provided by the present application is used to control the dyeing results of different batches after dyeing treatment, wherein A is the 5th batch, B is the 15th batch, C is the 25th batch, D is the 35th batch, and E is the 45th batch. Each batch contains 20 slides for simultaneous dyeing. According to the dyeing results, it can be known that by using the above-mentioned dyeing method provided by the present application, the pathological section dyeing can be uniform and stable, and the service life of the dyeing reagent can be also prolonged, and the dyeing cost can be effectively reduced.

[0079] The above is only an embodiment of the present application, and the protection scope of the present application is not limited by these specific embodiments, but is determined by the claims of the present application. The present application can have various changes and variations for those skilled in the art. Any modification, equivalent replacement, improvement, etc. within the technical thought and principle of the present application shall be included in the protection scope of the present application.

Claims

1. An automatic control method of pathological tissue staining, characterized by, The method comprises the following steps: S1: replacing the dyeing solution; S2: sequentially performing xylene dewaxing treatment, hematoxylin staining treatment, blueing solution treatment, and eosin staining treatment on the pathological tissue; S3: performing color data scanning on the pathological tissue after the staining treatment is completed, and calculating the staining quality; S4: judging whether the staining quality has a continuous downward trend: 1) If there is no continuous downward trend, return to S2 and process with the last hematoxylin staining time T he and eosin staining time T eo ; 2) If there is a continuous downward trend, then recalculate the hematoxylin staining processing time T he and eosin staining processing time T eo and determine whether the recalculated hematoxylin staining processing time T he and eosin staining processing time T eo exceeds the preset maximum value: 2.1) if the recalculated haematoxylin staining process time T he and eosin staining process time T eo exceeds a preset maximum value, then return to S1; 2.2) if the re-calculated haematoxylin staining process time T he and eosin staining process time T eo do not exceed the pre-set maximum values, then return to S2; In the step 2), the hematoxylin standard staining time is 2-4 min, and the eosin standard staining time is 8-12 s. Time T of treatment with hematoxylin he On the basis of the standard time T of treatment with hematoxylin b adjusted according to the quantity X of slides treated with hematoxylin since the last change of the staining solution he , the number of days D in which hematoxylin has been used he and AQ Eosin staining processing time T eo On the basis of eosin standard staining time T o According to the amount of slides X eo processed by eosin after the last change of staining solution, the number of days D eo eosin has been used, and ΔQ adjustment calculation is obtained; Wherein, AQ=Q best -Q bad , Q best is the dyeing quality after the last adjustment of the dyeing time, Q bad is the dyeing quality of this time; In the step S3, at least 8 fixed sampling points are selected on the slide after the staining is completed, color data is scanned and converted into an HLS color space, sorted according to brightness L, and 2-4 groups of data with the smallest L value are selected to calculate the staining quality.

2. The automatic control method of pathological tissue staining according to claim 1, characterized in that, The hematoxylin treatment time T he and eosin treatment time T eo The calculation method is: where X he is the amount of hematoxylin already treated slide after replacing the staining solution, pi = 1.122, p2 = -9.557, p3 = 14.85, p4 = 120.4, p5 = T b + AQ*300 + D he *5, T b is the hematoxylin standard staining time, D he is the number of days of hematoxylin already used; where X eo is the amount of Eosin processed slides after replacing the staining solution, p1 = 4.472, p2 = T o + ΔQ*10 + D eo , T o is the standard Eosin staining time, D eo is the number of days the Eosin has been used.

3. The automatic control method of pathological tissue staining according to claim 2, characterized in that, In the step S3, at least 8 fixed sampling points are selected on the slide after the staining is completed, color data is scanned and converted into an HLS color space, sorted according to brightness L, and 2-4 groups of data with the smallest L value are selected to calculate the staining quality.

4. The automatic control method of pathological tissue staining according to claim 3, characterized in that, The preset maximum value of the hematoxylin staining time is 10-14 min, and the preset maximum value of the eosin staining time is 1.5-2.5 min.

5. The automatic control method of pathological tissue staining according to claim 1, characterized in that, The step S2, xylene dewaxing treatment time T xf According to the ambient temperature adjustment, the blue liquid treatment time is 50-70s.

6. The automatic control method of pathological tissue staining according to claim 5, wherein, T xf = -18*T ev +720, where T ev is the ambient temperature.

7. The automatic control method of pathological tissue staining according to claim 6, wherein, The calculation method of the dyeing quality is: Q = (1 - L curr ) / (1 - L best ); L is the average value of L calculated from 2-4 groups of data with the minimum L value in the selected sampling points, wherein L best According to the dyeing quality after the first dyeing according to the standard dyeing time after the dyeing solution is replaced, wherein L curr According to the dyeing quality after the first dyeing according to the standard dyeing time after the dyeing solution is replaced, wherein L 8. The method of claim 1, wherein the method further comprises: In the step S2, the xylene dewaxing treatment is further followed by stable liquid immersion staining treatment before the hematoxylin staining treatment, and the stable liquid immersion staining treatment time is 5-10 min.

9. The method of claim 8, wherein the method further comprises: The stable liquid comprises a buffer solution, a polyhydroxy alcohol, and a microbial inhibitor, wherein the buffer solution is a citric acid solution or a hydrochloric acid solution, and the polyhydroxy alcohol is one or more of diols, triols, or polymers thereof.

10. The automatic control method of pathological tissue staining according to claim 9, wherein, The buffer solution is one of sodium phosphate dibasic-citric acid buffer solution, phthalic acid-hydrochloric acid buffer solution, and citric acid-sodium hydroxide-hydrochloric acid buffer solution. The polyhydroxy alcohol is one or more of propylene glycol, glycerol, dipropylene glycol, and polypropylene glycol. The microbial inhibitor is one or more of benzalkonium chloride, benzoic acid and its derivatives, and isothiazolinyl ketone bacteriostatic agent.

11. A dyeing machine system, characterized in that The staining machine system comprises the pathological tissue staining automatic control method according to any one of claims 1-10.

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