Biochemical reaction slide glass

Abstract

The utility model relates to a biochemical reaction slide glass, which comprises a substrate, a first electrode, a second electrode, a third electrode and a fourth electrode, the cover plate is arranged on one side of the substrate, and the cover plate and the substrate form a whole; wherein the substrate is provided with a first liquid inlet / outlet and a second liquid inlet / outlet, a confluence channel and at least two reaction tanks are arranged between the cover plate and the substrate, one end of each reaction tank is communicated with the first liquid inlet / outlet through the confluence channel, and the other end of each reaction tank is communicated with the second liquid inlet / outlet through the confluence channel. And / or the other end of each reaction tank is communicated with the second liquid inlet / outlet through the confluence channel. According to the biochemical reaction slide glass, the cover glass and the substrate form a whole, and the reaction tank is arranged between the cover glass and the substrate, so that the sealing performance and the integrity of the biochemical reaction slide glass are better; the confluence channel is arranged between the cover plate and the substrate, and the biochemical reaction slide glass has a confluence function, so that the structure of a liquid path system of the biochemical reaction slide glass is simplified, and the liquid path system is easy to maintain.

Description

Technical Field

[0001] The utility model relates to the field of biomedical engineering, in particular to a biochemical reaction slide. Background Art

[0002] Conventional biochemical reaction slides usually do not have a confluence structure. When the biochemical reaction slide is connected to the inlet block of an external liquid supply device, an independent pipeline needs to be set up in the inlet block, or an inlet block with an integrated confluence function needs to be used to connect the biochemical reaction slide with the inlet block channel. In this way, there will be a large number of redundant pipelines, resulting in waste of reagents in the common pipelines, and the liquid system structure of the biochemical reaction slide is complex and difficult to maintain. Utility Model Content

[0003] Based on this, it is necessary to provide a biochemical reaction slide to address the problem that the existing biochemical reaction slide liquid path system has a complex structure and is difficult to maintain.

[0004] A biochemical reaction slide, comprising:

[0005] substrate;

[0006] A cover sheet, which is disposed on one side of the substrate and forms an integral body with the substrate;

[0007] Among them, a first liquid inlet and outlet and a second liquid inlet and outlet are arranged on the substrate, and a confluence channel and at least two reaction grooves are arranged between the cover plate and the substrate, one end of each of the reaction grooves is connected to the first liquid inlet and outlet through the confluence channel, and / or the other end of each of the reaction grooves is connected to the second liquid inlet and outlet through the confluence channel.

[0008] In the above-mentioned biochemical reaction slide, the cover sheet and the substrate form an integral body, a reaction groove is arranged between the cover sheet and the substrate, and both ends of the reaction groove are connected with corresponding liquid inlets and outlets, so that the sealing and integrity of the biochemical reaction slide are better; a confluence channel is arranged between the cover sheet and the substrate, and the biochemical reaction slide itself has a confluence function, and the setting position of the confluence channel can be flexibly selected according to actual use requirements, which is beneficial to simplifying the structure of the liquid path system of the biochemical reaction slide and facilitating the maintenance of the liquid path system.

[0009] In one embodiment, each of the reaction grooves and the confluence channel is disposed on a side of the cover sheet facing the substrate, and / or on a side of the substrate facing the cover sheet.

[0010] In one embodiment, the biochemical reaction slide further comprises a structural membrane layer, wherein the structural membrane layer is disposed between the cover sheet and the substrate, and each of the reaction grooves and the confluence channel is disposed in the structural membrane layer.

[0011] In one embodiment, the structural film layer, the cover sheet and the substrate are bonded together to form a whole.

[0012] In one embodiment, the structural film layer is at least one of a double-sided adhesive film, a hot melt adhesive film or other dielectric films.

[0013] In one embodiment, the number of the first liquid inlet and outlet and the number of the second liquid inlet and outlet are both one; one end of each of the reaction tanks is connected to one of the first liquid inlet and outlet through the confluence channel, and the other end of each of the reaction tanks is connected to one of the second liquid inlet and outlet through the confluence channel.

[0014] In one embodiment, the number of the first liquid inlet and outlet is one, the number of the second liquid inlet and outlet is at least two, and the second liquid inlet and outlet correspond to the reaction tanks one by one; one end of each of the reaction tanks is connected to one of the first liquid inlet and outlet through the confluence channel, and the other end of each of the reaction tanks is connected to the corresponding second liquid inlet and outlet.

[0015] In one embodiment, the first liquid inlet and outlet and the second liquid inlet and outlet are spaced apart along a first direction, and the reaction grooves are spaced apart along a second direction intersecting the first direction, and the first direction is the length direction of the substrate.

[0016] In one embodiment, each of the reaction tanks includes a middle portion and two end portions, the two end portions are arranged opposite to each other along the first direction, the middle portion is located between the two end portions, and the flow area of ​​the middle portion is greater than the flow area of ​​any of the end portions.

[0017] In one embodiment, any of the end portions is wedge-shaped, and the middle portion is straight.

[0018] In one embodiment, the number of the first liquid inlet and outlet is one, and the number of the second liquid inlet and outlet is at least two; one end of each of the reaction tanks is connected to one of the first liquid inlet and outlet, and the other end of each of the reaction tanks is connected to the corresponding second liquid inlet and outlet through the confluence channel.

[0019] In one embodiment, the first liquid inlet and outlet and the second liquid inlet and outlet are spaced apart along a first direction, and the reaction slots are spaced apart along the first direction, and the first direction is the length direction of the substrate.

[0020] In one embodiment, each of the reaction tanks is arc-shaped.

[0021] In one embodiment, the height of each of the reaction grooves is equal to the height of the confluence channel, and the height of each of the reaction grooves and the height of the confluence channel are respectively in the range of 50µm to 600µm.

[0022] In one embodiment, the height of each reaction groove and the height of the confluence channel are 100 μm respectively.

[0023] In one embodiment, the height of each reaction groove and the height of the confluence channel are 500 μm respectively. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] FIG1 is a schematic diagram of a biochemical reaction slide in some embodiments provided in the present application.

[0025] FIG. 2 is an exploded view of the biochemical reaction slide shown in FIG. 1 .

[0026] FIG. 3 is a schematic diagram of the interior of a biochemical reaction slide in some embodiments.

[0027] FIG. 4 is a schematic diagram of the interior of a biochemical reaction slide in some other embodiments.

[0028] FIG. 5 is a schematic diagram of the interior of a biochemical reaction slide in yet other embodiments.

[0029] FIG. 6 is a schematic diagram of the interior of a biochemical reaction slide in yet other embodiments.

[0030] Reference numerals:

[0031] 10. Biochemical reaction carrier; 100. Base plate; 101. First liquid inlet and outlet; 102. Second liquid inlet and outlet; 103. Reaction tank; 103a. Middle part; 103b. End part; 104. Confluence channel; 104a. Main channel; 104b. Secondary channel; 200. Cover plate; 300. Structural membrane layer; 20. Liquid inlet block; 21. Liquid inlet channel. DETAILED DESCRIPTION

[0032] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0033] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "height", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0034] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0035] In this application, unless otherwise clearly specified and limited, the terms "initial", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected or indirectly connected through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0036] In the present application, unless otherwise clearly specified and limited, a first feature being “above” or “below” a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being “above”, “above”, and “above” a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being “below”, “below”, and “below” a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0037] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only implementation method.

[0038] Please refer to FIG. 1 and FIG. 2 , a biochemical reaction slide 10 in an embodiment includes a substrate 100 and a cover sheet 200, wherein the cover sheet 200 is disposed on one side of the substrate 100 and forms an integral body with the substrate 100. The substrate 100 is provided with a first liquid inlet and outlet 101 and a second liquid inlet and outlet 102, and a confluence channel 104 and at least two reaction grooves 103 are disposed between the cover sheet 200 and the substrate 100, wherein one end of each reaction groove 103 is connected to the first liquid inlet and outlet 101 by the confluence channel 104, and / or the other end of each reaction groove 103 is connected to the second liquid inlet and outlet 102 by the confluence channel 104.

[0039] It should be noted that the biochemical reaction slide 10 is used in the fields of biomedical research, drug screening, environmental monitoring, etc., for example, for high-throughput gene sequencing detection and various biochemical fluorescence reaction detection. The biochemical reaction slide 10 can be connected to an external liquid supply device, and the fluid medium supplied by the external liquid supply device can flow into the biochemical reaction slide 10, and a chemical reaction can occur in the biochemical reaction slide 10. Optionally, the first liquid inlet and outlet 101 and the second liquid inlet and outlet 102 can be used as both a liquid inlet of the fluid medium and a liquid outlet of the fluid medium.

[0040] For example, referring to FIG. 1 and FIG. 2 , the external liquid supply device has different liquid inlet blocks 20, each of which is provided with a liquid inlet channel 21, and the first liquid inlet and outlet port 101 and the second liquid inlet and outlet port 102 are connected to the corresponding liquid inlet channel 21. The first fluid medium flows into the reaction tank 103 through the first liquid inlet channel 21 and the first liquid inlet and outlet port 101, and the second fluid medium flows into the reaction tank 103 through the first liquid inlet channel 21 and the second liquid inlet and outlet port 102. The first fluid medium and the second fluid medium are mixed and chemically reacted in the reaction tank 103, and the chemical reaction product liquid in the reaction tank 103 is output from the second liquid inlet and outlet port 102. At this time, the first liquid inlet and outlet port 101 serves as the liquid inlet of the first fluid medium, and the second liquid inlet and outlet port 102 serves as the liquid inlet of the second fluid medium and the liquid outlet of the chemical reaction product liquid. Optionally, the first fluid medium can be a sample solution, and the second fluid medium can be a reagent solution.

[0041] In the above-mentioned biochemical reaction slide 10, the cover sheet 200 and the substrate 100 form an integral body, a reaction groove 103 is arranged between the cover sheet 200 and the substrate 100, and both ends of the reaction groove 103 are connected with corresponding liquid inlets and outlets, so that the sealing and integrity of the biochemical reaction slide 10 are better; a confluence channel 104 is arranged between the cover sheet 200 and the substrate 100, and the biochemical reaction slide 10 itself has a confluence function, and the setting position of the confluence channel 104 can be flexibly selected according to actual use requirements, which is beneficial to simplifying the structure of the liquid path system of the biochemical reaction slide 10 and facilitating the maintenance of the liquid path system.

[0042] In this embodiment, the substrate 100 is a precisely processed polished wafer, a glass wafer or a polymer substrate. The substrate 100 is a thin sheet structure, and the outer contour of the substrate 100 can be a rectangle, a circle or other irregular shapes.

[0043] In this embodiment, the cover sheet 200 is a high-transmittance glass, fused quartz or single-crystal quartz with low fluorescence background. The cover sheet 200 is a thin sheet structure, and the outer contour of the cover sheet 200 can be a rectangle, a circle or other irregular shapes.

[0044] In this embodiment, the cover sheet 200 and the substrate 100 can be packaged as a whole by gluing or bonding. Here, the method of integrally forming the cover sheet 200 and the substrate 100 is not specifically limited.

[0045] In this embodiment, the number of confluence channels 104 between each reaction tank 103 and the corresponding liquid inlet and outlet is not limited to one, that is, the number of confluence channels 104 can be at least two, and each confluence channel 104 can be straight or arc-shaped or other irregular shapes. The number and shape of the confluence channels 104 are not specifically limited here.

[0046] 1, each reaction slot 103 and confluence channel 104 is disposed on the side of the cover sheet 200 facing the substrate 100, and / or disposed on the side of the substrate 100 facing the cover sheet 200. This facilitates the simplification of the structure of the biochemical reaction slide 10 and the maintenance of the liquid path system.

[0047] It can be understood that each reaction groove 103 and confluence channel 104 can be arranged on the side of the cover plate 200 facing the substrate 100, or can be arranged on the side of the substrate 100 facing the cover plate 200; or a part of each reaction groove 103 and confluence channel 104 is arranged on the side of the cover plate 200 facing the substrate 100, and the other part is arranged on the side of the substrate 100 facing the cover plate 200, when the substrate 100 and the cover plate 200 are covered, a complete reaction groove 103 and confluence channel 104 can be formed.

[0048] In this embodiment, each reaction groove 103 and each confluence channel 104 may be disposed on a side of the cover sheet 200 facing the substrate 100 and / or disposed on a side of the substrate 100 facing the cover sheet 200 by etching.

[0049] Specifically in other embodiments, please refer to FIG. 2 , the biochemical reaction slide 10 further includes a structural film layer 300, the structural film layer 300 is disposed between the cover sheet 200 and the substrate 100, and each reaction slot 103 and the confluence channel 104 are disposed in the structural film layer 300. In this way, by arranging each reaction slot 103 and the confluence channel 104 in the same plane of the structural film layer 300 without any special additional structure, the volume of the common distribution pipeline (i.e., the confluence channel 104) is minimized while ensuring uniform distribution of the fluid in each reaction slot 103.

[0050] It should be noted that after the structural film layer 300 is sandwiched between the cover sheet 200 and the substrate 100 , a sealing process is performed between the cover sheet 200 and the substrate 100 to enable the cover sheet 200 and the substrate 100 to be sealed.

[0051] In this embodiment, the structural film layer 300, the cover sheet 200 and the substrate 100 can be packaged as a whole by glue adhesion or bonding connection. Optionally, the bonding connection is molecular diffusion bonding or anodic bonding.

[0052] In this embodiment, please refer to FIG. 2 , the structural membrane layer 300 is at least one of a double-sided adhesive film, a hot melt adhesive film or other dielectric films. In this way, the material of the structural membrane layer 300 can be flexibly selected according to actual use requirements to take into account the convenience of assembling the structural membrane layer 300 and the convenience of setting the reaction tank 103 and the confluence channel 104.

[0053] In this embodiment, the structural film layer 300 is a double-sided adhesive film or a hot melt adhesive film. In other embodiments, the structural film layer 300 can also be other structural layers based on MEMS technology, such as a structural layer formed on the cover film 200 or the substrate surface by etching or machining.

[0054] As shown in some embodiments of FIG. 3 , the number of the first liquid inlet and outlet 101 and the number of the second liquid inlet and outlet 102 are both one; one end of each reaction tank 103 is connected to a first liquid inlet and outlet 101 through a confluence channel 104, and the other end of each reaction tank 103 is connected to a second liquid inlet and outlet 102 through a confluence channel 104.

[0055] It should be noted that one end of each reaction tank 103 is connected to a first liquid inlet and outlet 101 through a confluence channel 104, and the other end of each reaction tank 103 is connected to a second liquid inlet and outlet 102 through a confluence channel 104, so as to realize one-in-one-out of the reaction tank 103; this is beneficial to the simplification of the structure of the biochemical reaction slide 10 and the maintenance of the liquid path system.

[0056] For example, when the number of reaction tanks 103 is four, there are two confluence channels 104 between one end of the four reaction tanks 103 and a first liquid inlet and outlet 101, each confluence channel 104 includes a main channel 104a and two side channels 104b, the main channel 104a connects the first liquid inlet and outlet 101 and the side channel 104b, and each side channel 104b is connected to one end of a corresponding reaction tank 103; there are two confluence channels 104 between the other end of the four reaction tanks 103 and a second liquid inlet and outlet 102, and the specific layout is similar to that between one end of the reaction tank 103 and the first liquid inlet and outlet 101, which will not be repeated here. Here, after the fluid medium is input through the first liquid inlet and outlet 101, it is divided into two confluence channels 104, and then divided into four reaction tanks 103, and then converged into two confluence channels 104, and finally converged into a second liquid inlet and outlet 102 for output.

[0057] As shown in some other embodiments of FIG. 4 , the number of the first liquid inlet and outlet ports 101 is one, the number of the second liquid inlet and outlet ports 102 is at least two, and the second liquid inlet and outlet ports 102 correspond one-to-one to the reaction tanks 103; one end of each reaction tank 103 is connected to a first liquid inlet and outlet port 101 through a confluence channel 104, and the other end of each reaction tank 103 is connected to the corresponding second liquid inlet and outlet port 102.

[0058] It should be noted that one end of each reaction tank 103 is connected to a first liquid inlet and outlet 101 through a confluence channel 104, and the other end of each reaction tank 103 is connected to the corresponding second liquid inlet and outlet 102, so as to realize one-inlet and multiple-outlet of the reaction tank 103; in this way, on the basis of ensuring uniform fluid distribution in each reaction tank 103, it is beneficial to minimize the volume of the common distribution pipeline (that is, the confluence channel 104).

[0059] For example, when the number of reaction tanks 103 is four, the number of second liquid inlet and outlet ports 102 is four; there are two confluence channels 104 between one end of the four reaction tanks 103 and one first liquid inlet and outlet port 101, each confluence channel 104 includes a main channel 104a and two side channels 104b, the main channel 104a connects the first liquid inlet and outlet port 101 and the side channels 104b, each side channel 104b is connected to one end of a corresponding reaction tank 103, and the other end of each reaction tank 103 is connected to the corresponding second liquid inlet and outlet port 102. Here, after the fluid medium is input through the first liquid inlet and outlet port 101, it is divided into the two confluence channels 104, and then divided into the four reaction tanks 103, and finally output from each second liquid inlet and outlet port 102 corresponding to each reaction tank 103.

[0060] In the above embodiment, referring to FIG3 and FIG4 , the first liquid inlet and outlet 101 and the second liquid inlet and outlet 102 are spaced apart along a first direction, and the reaction slots 103 are spaced apart along a second direction intersecting the first direction, and the first direction is the length direction of the substrate 100. In this way, the space utilization rate of the biochemical reaction slide 10 is improved, and the volume of the biochemical reaction slide 10 is miniaturized.

[0061] It should be noted that the first direction is the X direction shown in Figures 3 and 4, that is, the length direction of the substrate 100; the second direction is the Y direction shown in Figures 3 and 4, that is, the width direction of the substrate 100, and the second direction is perpendicular to the first direction.

[0062] For example, when there are four reaction tanks 103, the first liquid inlet and outlet 101 and the second liquid inlet and outlet 102 are arranged side by side and spaced apart along the first direction, and the four reaction tanks 103 are arranged side by side and spaced apart along the second direction. The four reaction tanks 103 are arranged at equal intervals to facilitate uniform distribution of fluid in each reaction tank 103.

[0063] In this embodiment, each reaction groove 103 extends along the first direction, and each reaction groove 103 has exactly the same shape and size, so as to facilitate uniform distribution of fluid in each reaction groove 103 .

[0064] Specifically, please refer to Figures 3 and 4. Each reaction tank 103 includes a middle portion 103a and two end portions 103b. The two end portions 103b are arranged opposite to each other along a first direction. The middle portion 103a is located between the two end portions 103b. The flow area of ​​the middle portion 103a is greater than the flow area of ​​any end portion 103b.

[0065] It should be noted that the flow area of ​​the middle portion 103a and the flow area of ​​the end portion 103b are both cross-sectional areas on the XY plane shown in FIG. 3 and FIG. 4, and the XY plane is perpendicular to the Z axis. Each reaction tank 103 extends along the first direction (the X direction shown in FIG. 3 and FIG. 4), and the two end portions 103b of each reaction tank 103 are arranged relatively along the first direction, and the flow area of ​​the middle portion 103a is greater than the flow area of ​​any end portion 103b. In this way, the flow velocity of the fluid medium when flowing through the two end portions 103b can be accelerated, which is conducive to the fluid medium to quickly flow in or out from the two end portions 103b.

[0066] More specifically, referring to FIG. 3 and FIG. 4 , any end portion 103 b is wedge-shaped, and the middle portion 103 a is straight-bar-shaped.

[0067] It can be understood that any end 103b has a first end side and a second end side, the first end side faces each liquid inlet and outlet, and the second end side is connected to the middle part 103a; in the second direction (Y direction shown in Figures 3 and 4), the size of the first end side is smaller than the size of the second end side, so that the end 103b is wedge-shaped.

[0068] In this embodiment, the middle portion 103a is in a straight strip shape, the second end side is connected to the middle portion 103a, and the size of the second end side in the second direction is equal to the size of the middle portion 103a in the second direction. In other embodiments, the size of the second end side in the second direction may not be equal to the size of the middle portion 103a in the second direction.

[0069] As shown in some other embodiments of Figures 5-6, the number of the first liquid inlet and outlet ports 101 is one, and the number of the second liquid inlet and outlet ports 102 is at least two; one end of each reaction tank 103 is connected to a first liquid inlet and outlet port 101, and the other end of each reaction tank 103 is connected to the corresponding second liquid inlet and outlet port 102 through a confluence channel 104.

[0070] It should be noted that one end of each reaction tank 103 is connected to a first liquid inlet and outlet 101, and the other end of each reaction tank 103 is connected to the corresponding second liquid inlet and outlet 102 through a confluence channel 104, so as to realize one-inlet and multiple-outlet of the reaction tank 103; in this way, on the basis of ensuring uniform fluid distribution in each reaction tank 103, it is beneficial to minimize the volume of the common distribution pipeline (that is, the confluence channel 104).

[0071] For example, when the number of reaction tanks 103 is two, the number of second liquid inlet and outlet ports 102 is four, one end of the two reaction tanks 103 is connected to one first liquid inlet and outlet port 101, and a confluence channel 104 is provided between the other end of each reaction tank 103 and one second liquid inlet and outlet port 102. Here, after the fluid medium is input through the first liquid inlet and outlet port 101, it is divided into the two reaction tanks 103, and then divided into the four confluence channels 104, and finally output from each second liquid inlet and outlet port 102 corresponding to each confluence channel 104.

[0072] In the above embodiment, please refer to FIG5 and FIG6 , the first liquid inlet and outlet 101 and the second liquid inlet and outlet 102 are spaced apart along the first direction, and the reaction tanks 103 are spaced apart along the first direction, and the first direction is the length direction of the substrate 100. In this way, the space utilization rate of the biochemical reaction slide 10 is improved, and the volume of the biochemical reaction slide 10 is miniaturized.

[0073] It should be noted that the first direction is the X direction shown in FIG. 5 and FIG. 6 , that is, the length direction of the substrate 100 ; the reaction grooves 103 are distributed at intervals along the length direction of the substrate 100 .

[0074] Specifically, please refer to FIG. 5 and FIG. 6 , each reaction tank 103 is arc-shaped.

[0075] In this embodiment, the shapes and sizes of the reaction grooves 103 are exactly the same, for example, they are arc-shaped structures with the same curvature. In other embodiments, the shapes and sizes of the reaction grooves 103 may also be different.

[0076] Please refer to FIG. 1 . The height of each reaction groove 103 is equal to the height of the confluence channel 104 . The height of each reaction groove 103 and the height of the confluence channel 104 are in the range of 50 μm to 600 μm, respectively.

[0077] It should be noted that the height direction is the Z direction shown in FIG1 . When the height of each reaction groove 103 and the height of the confluence channel 104 are too large, the biochemical reaction slide 10 will occupy a large space and the flow rate of the confluence channel 104 and the reaction groove 103 will be too slow; when the height of each reaction groove 103 and the height of the confluence channel 104 are too small, it will be difficult to set up the confluence channel 104 and the reaction groove 103. According to the results of the simulation test, the height of each reaction groove 103 and the height of the confluence channel 104 are limited to 50µm-600µm, which can take into account the setting of the confluence channel 104 and the reaction groove 103 and the fluid flow rate.

[0078] Specifically in one embodiment, please refer to FIG. 1 , the height of each reaction groove 103 and the height of the confluence channel 104 are 100 μm respectively.

[0079] Specifically in another embodiment, please refer to FIG. 1 , the height of each reaction groove 103 and the height of the confluence channel 104 are 500 μm respectively.

[0080] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0081] The above-mentioned embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the utility model patent. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application shall be subject to the attached claims.

Claims

1. A biochemical reaction slide (10), characterized in that: include: A substrate (100); A cover sheet (200) is disposed on one side of the substrate (100) and forms an integral body with the substrate (100); Wherein, a first liquid inlet and outlet (101) and a second liquid inlet and outlet (102) are provided on the substrate (100), and a confluence channel (104) and at least two reaction grooves (103) are provided between the cover plate (200) and the substrate (100), one end of each reaction groove (103) is connected to the first liquid inlet and outlet (101) through the confluence channel (104), and / or the other end of each reaction groove (103) is connected to the second liquid inlet and outlet (102) through the confluence channel (104).

2. The biochemical reaction slide (10) according to claim 1, characterized in that: Each of the reaction grooves (103) and the confluence channel (104) is arranged on a side of the cover sheet (200) facing the substrate (100), and / or is arranged on a side of the substrate (100) facing the cover sheet (200).

3. The biochemical reaction slide (10) according to claim 2, characterized in that: The biochemical reaction slide (10) further comprises a structural membrane layer (300), wherein the structural membrane layer (300) is arranged between the cover sheet (200) and the substrate (100), and each of the reaction grooves (103) and the confluence channel (104) is arranged on the structural membrane layer (300).

4. The biochemical reaction slide (10) according to claim 3, characterized in that: The structural film layer (300), the cover sheet (200) and the base sheet (100) are formed into a whole by bonding.

5. The biochemical reaction slide (10) according to claim 3, characterized in that: The structural film layer (300) is at least one of a double-sided adhesive film, a hot-melt adhesive film or other dielectric films.

6. The biochemical reaction slide (10) according to claim 1, characterized in that: The number of the first liquid inlet and outlet (101) and the number of the second liquid inlet and outlet (102) are both one; One end of each of the reaction grooves (103) is connected to one of the first liquid inlet and outlet ports (101) through the confluence channel (104), and the other end of each of the reaction grooves (103) is connected to one of the second liquid inlet and outlet ports (102) through the confluence channel (104).

7. The biochemical reaction slide (10) according to claim 1, characterized in that: The number of the first liquid inlet and outlet (101) is one, the number of the second liquid inlet and outlet (102) is at least two, and the second liquid inlet and outlet (102) corresponds one-to-one to the reaction tank (103); One end of each reaction tank (103) is connected to one of the first liquid inlet and outlet ports (101) via the confluence channel (104), and the other end of each reaction tank (103) is connected to the corresponding second liquid inlet and outlet port (102).

8. The biochemical reaction slide (10) according to claim 6 or 7, characterized in that: The first liquid inlet and outlet (101) and the second liquid inlet and outlet (102) are spaced apart along a first direction, and the reaction grooves (103) are spaced apart along a second direction intersecting the first direction, wherein the first direction is the length direction of the substrate (100).

9. The biochemical reaction slide (10) according to claim 8, characterized in that: Each of the reaction tanks (103) includes a middle portion (103a) and two end portions (103b), the two end portions (103b) are arranged opposite to each other along the first direction, the middle portion (103a) is located between the two end portions (103b), and the flow area of ​​the middle portion (103a) is greater than the flow area of ​​any of the end portions (103b).

10. The biochemical reaction slide (10) according to claim 9, characterized in that: Any of the end portions (103b) is wedge-shaped, and the middle portion (103a) is straight.

11. The biochemical reaction slide (10) according to claim 1, characterized in that: The number of the first liquid inlet and outlet (101) is one, and the number of the second liquid inlet and outlet (102) is at least two; One end of each reaction tank (103) is connected to one of the first liquid inlet and outlet ports (101), and the other end of each reaction tank (103) is connected to the corresponding second liquid inlet and outlet port (102) via the confluence channel (104).

12. The biochemical reaction slide (10) according to claim 11, characterized in that: The first liquid inlet and outlet (101) and the second liquid inlet and outlet (102) are spaced apart along a first direction, and the reaction grooves (103) are spaced apart along the first direction, and the first direction is the length direction of the substrate (100).

13. The biochemical reaction slide (10) according to claim 11, characterized in that: Each of the reaction tanks (103) is arc-shaped.

14. The biochemical reaction slide (10) according to claim 1, characterized in that: The height of each reaction groove (103) is equal to the height of the confluence channel (104), and the height of each reaction groove (103) and the height of the confluence channel (104) are respectively in the range of 50 μm to 600 μm.

15. The biochemical reaction slide (10) according to claim 1, characterized in that: The height of each reaction groove (103) and the height of the confluence channel (104) are 100 μm respectively.

16. The biochemical reaction slide (10) according to claim 1, characterized in that: The height of each reaction groove (103) and the height of the confluence channel (104) are 500 μm respectively.

Images