Synthesis gas fermentation strain fed-batch system and fermentation assembly

Abstract

The utility model relates to a syngas fermentation strain fed-batch system and a fermentation assembly. The fed-batch system comprises a distiller's yeast tank and a fermentation assembly, the third-stage strain expanding culture unit is connected with a culture medium feeding hole of the distiller's yeast tank; the deoxygenated water unit is connected with the upper part of the distiller's yeast tank; the nitrogen sealing pipeline unit and the tail gas treatment pipeline unit are respectively connected with the top of the distiller's yeast tank; the fermentation liquor circulating cooling unit is connected with the bottom of the distiller's yeast tank and forms circulation; the CO gas inlet unit is connected with the distiller's yeast tank; the chemical adding unit and the nutrient solution adding unit are connected with the fermentation liquor circulating cooling unit; and the material conveying pipeline unit is connected with the bottom of the distiller's yeast tank and outputs outwards. By adopting the fed-batch system, the service life of strains in the fermentation tank can be prolonged, and the spreading culture frequency of the strains can be reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of synthetic gas fermentation, and relates to a synthetic gas fermentation bacteria feed system and a fermentation component. Background Art

[0002] Syngas fermentation is the process of anaerobic aerobic bacteria using syngas (CO, CO2 and H2) as a carbon source and energy source to reproduce and grow. Depending on the type of bacteria, they metabolize to produce biofuels such as ethanol and butanol. Anaerobic aerobic bacteria exist in nature, and the strains can be artificially selected and domesticated; syngas is widely derived from coal chemical industry, petrochemical industry, industrial waste gas (steel plant waste gas, etc.), urban garbage and biomass. Therefore, applying syngas as a carbon source and energy source to industrial fermentation of microorganisms can not only produce green energy and improve the economic benefits of enterprises, but also reduce the emission of carbon-containing gases, alleviate the greenhouse effect, and create environmental benefits for society.

[0003] At present, syngas fermentation technology is developing rapidly and has achieved industrial production in China. Compared with the active dry yeast technology used in the traditional culture of food-based ethanol strains, the current culture of aerobic bacteria is still in the three-stage expansion stage, which requires culture from test tubes or triangular flasks. According to the proportion of the culture medium, each level requires a long culture time to reach the required cell concentration before the next level of expansion culture can be carried out. Active dry yeast technology has not yet been applied to the culture of aerobic bacteria.

[0004] After the three-stage expansion of the strain, the strain will eventually reach the required strain concentration, and will be inoculated into the fermentation tank at one time. The strain will reproduce and grow in the fermentation tank, and the fermentation activity will produce ethanol and other bioenergy. After the fermentation tank runs for about 1500 hours, the strain fermentation activity decays and the product conversion rate decreases. At this time, it is necessary to re-carry out the three-stage expansion activity of the strain, and empty the fermentation tank for re-inoculation and cultivation. Chinese patent documents CN107779477A "A culture system and culture method for anaerobic gas-eating bacteria to produce alcohol by fermentation of synthetic gas" and CN106148426A "A culture system and culture method for anaerobic gas-eating bacteria to produce alcohol by fermentation containing molybdenum, zinc and nickel" both mention in detail the culture system and culture method of anaerobic gas-eating bacteria, so that the concentration of the strain and the conversion rate of the target product are improved.

[0005] However, the above utility model only focuses on the culture system and culture method of anaerobic aerobic bacteria, and does not study the use method and process of mature bacteria. Designing a process that can extend the service life of fermentation tank bacteria and reduce the number of times the bacteria are expanded is of great significance to promoting syngas fermentation technology when anaerobic aerobic bacteria cannot be cultivated using industrial active dry yeast technology. Utility Model Content

[0006] The purpose of the utility model is to provide a synthesis gas fermentation bacteria flow addition system to extend the service life of the bacteria in the fermentation tank, reduce the number of times of bacteria expansion and the like.

[0007] The purpose of the utility model can be achieved through the following technical solutions:

[0008] One of the technical solutions of the utility model provides a synthesis gas fermentation bacteria feed system, comprising:

[0009] yeast jar;

[0010] A three-stage bacterial strain expansion unit connected to the culture medium feed port of the yeast tank;

[0011] A deoxygenated water unit connected to the upper part of the yeast tank;

[0012] Respectively connect the nitrogen sealing pipeline unit and the tail gas treatment pipeline unit on the top of the yeast tank;

[0013] A fermentation liquid circulation cooling unit connected to the bottom of the yeast tank to form a circulation;

[0014] A CO gas inlet unit connected to the yeast tank;

[0015] A chemical adding unit and a nutrient solution adding unit connected to the fermentation liquid circulation cooling unit;

[0016] And a material conveying pipeline unit connected to the bottom of the yeast tank and outputting externally.

[0017] Furthermore, the three-stage bacterial strain expansion unit includes three bacterial strain expansion tanks connected in series, and the last bacterial strain expansion tank is also connected to the culture medium feed port through a culture medium feed pipe.

[0018] Furthermore, the deoxygenated water unit includes a water inlet arranged on the yeast tank, and a deoxygenated water pipeline connected to the water inlet.

[0019] Furthermore, the nitrogen sealing pipeline unit includes a nitrogen inlet arranged on the top of the yeast tank, and a nitrogen pipeline connected to the nitrogen inlet.

[0020] Furthermore, the fermentation liquid circulation cooling unit includes a circulation pipeline connected to the bottom of the yeast tank and forming a circulation, and a heat exchanger arranged on the circulation pipeline.

[0021] Furthermore, the chemical addition unit includes a chemical addition port arranged on the circulation pipeline, and a chemical pipeline connected to the chemical addition port;

[0022] The nutrient solution adding unit comprises a nutrient solution adding port arranged on the circulation pipeline, and a nutrient solution pipeline connected to the nutrient solution adding port.

[0023] Furthermore, the material conveying pipeline unit includes a discharge port arranged at the bottom of the yeast tank, a discharge pipeline connected to the discharge port, and a discharge pump arranged on the discharge pipeline.

[0024] Furthermore, the CO air inlet unit includes a CO air inlet port arranged on the yeast tank, and a CO air inlet pipeline connected to the CO air inlet port.

[0025] Furthermore, the volume of the yeast tank is 10-20% of that of the fermentation tank.

[0026] Furthermore, a biological reaction unit is also provided in the yeast tank.

[0027] A second technical solution of the present invention provides a fermentation component, which includes a fermentation tank and a synthesis gas fermentation strain flow-addition system as described above and connected to the fermentation tank.

[0028] Compared with the prior art, the utility model has the following advantages:

[0029] (1) The utility model is a device that adds a yeast tank after the anaerobic aerobic bacteria strains are expanded for three stages, as a device for continuous flow-feeding and culturing the bacteria strains when the fermentation tank is operating normally. The device is different from the normal feed of the fermentation tank. It uses a certain concentration of CO instead of synthesis gas to provide a carbon source for the aerobic bacteria strains, and uses fresh deoxygenated water instead of the secondary clarified liquid as the culture medium of the fermentation liquid. The pure CO and deoxygenated water provide a relatively sufficient energy source for the bacteria strains to reproduce, and reduce the chance of bacterial contamination in the yeast tank.

[0030] (2) The flow-feeding process of the yeast tank to the fermentation tank can provide a continuous supply of fresh fermentation bacteria for the fermentation tank, extend the service life of the bacteria in the fermentation tank, reduce the frequency of tank turnover, and reduce labor and material consumption. The service life of normal bacteria is about 1500 hours. After adding the flow-feeding process of the yeast tank, it is expected that the service life of the bacteria can be increased to more than 4000 hours. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a schematic diagram of the structure of the utility model;

[0032] Description of the markings in the figure:

[0033] 1-yeast tank, 2-three-stage bacterial expansion unit, 3-culture medium delivery pipe, 4-culture medium feed port, 5-fermentation liquid circulation cooling unit, 6-heat exchanger, 7-circulation pipeline, 8-circulation liquid feed port, 9-CO inlet pipeline, 10-CO inlet, 11-chemical pipeline, 12-chemical addition port, 13-nutrient solution pipeline, 14-nutrient solution addition port, 15-deoxygenated water pipeline, 16-water inlet, 17-exhaust gas outlet pipeline, 18-exhaust gas outlet, 19-nitrogen pipeline, 20-nitrogen inlet, 21-discharge port, 22-discharge pipeline, 23-discharge pump, 24-bioreactor unit. DETAILED DESCRIPTION

[0034] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments. This embodiment is implemented based on the technical solution of the present invention, and provides a detailed implementation method and a specific operation process, but the protection scope of the present invention is not limited to the following embodiments.

[0035] In the following implementation manners or examples, unless otherwise specified, functional components or structures are conventional components or conventional structures used in the art to achieve corresponding functions.

[0036] In order to extend the service life of the fermentation tank strains and reduce the number of times the strains are expanded, the utility model provides a synthetic gas fermentation strain feeding system, the structure of which is shown in Figure 1 As shown, including:

[0037] Wine yeast tank 1;

[0038] A strain three-stage expansion unit 2 connected to the culture medium feed port 4 of the yeast tank 1;

[0039] A deoxygenated water unit connected to the upper part of the yeast tank 1;

[0040] Respectively connect the nitrogen sealing pipeline unit and the tail gas treatment pipeline unit on the top of the yeast tank 1;

[0041] A fermentation liquid circulation cooling unit 5 connected to the bottom of the yeast tank 1 and forming a circulation;

[0042] A CO gas inlet unit connected to the yeast tank 1;

[0043] A chemical adding unit and a nutrient solution adding unit connected to the fermentation liquid circulation cooling unit 5;

[0044] And a material conveying pipeline unit connected to the bottom of the yeast tank 1 and outputting to the outside.

[0045] In some specific embodiments, the strain three-stage expansion unit 2 includes three strain expansion tanks connected in series, and the last strain expansion tank is also connected to the culture medium feed port 4 through a culture medium feed pipe 3, and feeds into the yeast tank 1 through the culture medium feed port 4. The structure of the strain three-stage expansion unit 2 itself does not belong to the innovative protection point of the present utility model, and it can adopt the conventional structure in the field.

[0046] In some specific embodiments, the deoxygenated water unit includes a water inlet 16 disposed on the yeast tank 1 , and a deoxygenated water pipeline 15 connected to the water inlet 16 .

[0047] In some specific embodiments, the nitrogen sealing pipeline unit includes a nitrogen inlet 20 disposed on the top of the yeast tank 1 , and a nitrogen pipeline 19 connected to the nitrogen inlet 20 , and nitrogen enters the yeast tank 1 through the nitrogen inlet 20 .

[0048] In some specific embodiments, the fermentation liquid circulation cooling unit 5 includes a circulation pipeline 7 connected to the bottom of the yeast tank 1 and forming a circulation, and a heat exchanger 6 arranged on the circulation pipeline 7, and is connected to a circulation liquid feed port 8 through the circulation pipeline 7, and the circulation liquid returns to the yeast tank 1 through the circulation liquid feed port 8.

[0049] In a more specific embodiment, the chemical adding unit includes a chemical adding port 12 arranged on the circulation pipeline 7 and a chemical pipeline 11 connected to the chemical adding port 12 , and different chemicals enter the circulation pipeline 7 through the chemical adding port 12 .

[0050] In a more specific embodiment, the nutrient solution adding unit includes a nutrient solution adding port 14 arranged on the circulation pipeline 7 , and a nutrient solution pipeline 13 connected to the nutrient solution adding port 14 .

[0051] In some specific embodiments, the material conveying pipeline unit includes a discharge port 21 arranged at the bottom of the yeast tank 1, a discharge pipeline 22 connected to the discharge port 21, and a discharge pump 23 arranged on the discharge pipeline 22, and the discharge pump 23 conveys the culture solution to the fermentation tank.

[0052] In some specific embodiments, the CO gas inlet unit includes a CO gas inlet 10 disposed on the yeast tank 1 , and a CO gas inlet pipeline 9 connected to the CO gas inlet 10 .

[0053] In some specific embodiments, the exhaust gas treatment pipeline unit includes an exhaust gas outlet 18 arranged on the yeast tank 1, and an exhaust gas outlet pipeline 17 connected to the exhaust gas outlet 18. The unconverted CO is converted into exhaust gas and output from the yeast tank 1 through the exhaust gas outlet 18.

[0054] In some specific embodiments, the volume of the yeast tank 1 is 10-20% of the fermentation tank. It is a pressure vessel with upper and lower head designs, and its volume is preferably 10% (or larger) of the fermentation tank, serving as a place for receiving mature strains after the third-stage expansion culture for continued cultivation.

[0055] In some specific embodiments, the yeast tank 1 is further provided with a bioreactor unit 24. Preferably, the bioreactor unit 24 is also connected to the fermentation liquid circulation cooling unit 5 and the CO air intake unit, respectively, to mix the circulating fermentation liquid and the CO gas, so that the bacteria can digest and convert CO. Specifically, the bioreactor unit 24 is mainly used for mixing the circulating fermentation liquid with the CO gas. The bioreactor 24 used in the present invention has a liquid phase inlet and a gas phase inlet respectively connected to the circulating fermentation liquid and the CO gas, and is a generator that can generate microbubbles after gas-liquid mixing. Here, an existing known mixing reactor that can generate microbubbles can be used, which itself does not belong to the innovative protection point of the present utility model.

[0056] The above embodiments may be implemented individually or in any combination of two or more.

[0057] The above implementation is described in more detail below in conjunction with specific examples.

[0058] Embodiment 1:

[0059] In order to extend the service life of the fermentation tank strains and reduce the number of times the strains are expanded, this embodiment provides a synthesis gas fermentation strain feeding system, the structure of which is shown in FIG. Figure 1 As shown, including:

[0060] Wine yeast tank 1;

[0061] A strain three-stage expansion unit 2 connected to the culture medium feed port 4 of the yeast tank 1;

[0062] A deoxygenated water unit connected to the upper part of the yeast tank 1;

[0063] Respectively connect the nitrogen sealing pipeline unit and the tail gas treatment pipeline unit on the top of the yeast tank 1;

[0064] A fermentation liquid circulation cooling unit 5 connected to the bottom of the yeast tank 1 and forming a circulation;

[0065] A CO gas inlet unit connected to the yeast tank 1;

[0066] A chemical adding unit and a nutrient solution adding unit connected to the fermentation liquid circulation cooling unit 5;

[0067] And a material conveying pipeline unit connected to the bottom of the yeast tank 1 and outputting to the outside.

[0068] The three-stage bacterial strain expansion unit 2 includes three bacterial strain expansion tanks connected in series, and the last bacterial strain expansion tank is also connected to the culture medium feed port 4 through the culture medium feed pipe 3, and feeds into the yeast tank 1 through the culture medium feed port 4.

[0069] The deoxygenated water unit includes a water inlet 16 disposed on the yeast tank 1 and a deoxygenated water pipeline 15 connected to the water inlet 16 .

[0070] The nitrogen sealing pipeline unit includes a nitrogen inlet 20 arranged on the top of the yeast tank 1, and a nitrogen pipeline 19 connected to the nitrogen inlet 20, and nitrogen enters the yeast tank 1 through the nitrogen inlet 20.

[0071] The fermentation liquid circulation cooling unit 5 includes a circulation pipeline 7 connected to the bottom of the yeast tank 1 and forming a circulation, and a heat exchanger 6 arranged on the circulation pipeline 7, and is connected to a circulation liquid feed port 8 through the circulation pipeline 7, and the circulation liquid returns to the yeast tank 1 through the circulation liquid feed port 8.

[0072] The chemical adding unit comprises a chemical adding port 12 arranged on the circulation pipeline 7 and a chemical pipeline 11 connected to the chemical adding port 12 . Different chemicals enter into the circulation pipeline 7 through the chemical adding port 12 .

[0073] The nutrient solution adding unit includes a nutrient solution adding port 14 arranged on the circulation pipeline 7 and a nutrient solution pipeline 13 connected to the nutrient solution adding port 14 .

[0074] The material conveying pipeline unit includes a discharge port 21 arranged at the bottom of the yeast tank 1, a discharge pipeline 22 connected to the discharge port 21, and a discharge pump 23 arranged on the discharge pipeline 22, and the discharge pump 23 conveys the culture solution to the fermentation tank.

[0075] The CO air inlet unit includes a CO air inlet 10 disposed on the yeast tank 1 , and a CO air inlet pipeline 9 connected to the CO air inlet 10 .

[0076] The tail gas treatment pipeline unit includes a tail gas outlet 18 arranged on the yeast tank 1, and a tail gas outlet pipeline 17 connected to the tail gas outlet 18. The unconverted CO is converted into tail gas and output from the yeast tank 1 through the tail gas outlet 18.

[0077] The volume of the yeast tank 1 is 10-20% of the fermentation tank. It is a pressure vessel with upper and lower head designs. The volume is preferably 10% (or larger) of the fermentation tank. It serves as a place to receive the mature strains after the third-level expansion culture and continue to culture.

[0078] The yeast tank 1 is also provided with a bioreactor unit 24. Preferably, the bioreactor unit 24 is also connected to the fermentation liquid circulation cooling unit 5 and the CO air intake unit, respectively, to mix the circulating fermentation liquid and the CO gas, so that the bacteria can digest and convert CO. Specifically, the bioreactor unit 24 is mainly used for mixing the circulating fermentation liquid with the CO gas. The bioreactor 24 used in this embodiment has a gas phase inlet and a liquid phase inlet. After the CO gas and the circulating fermentation liquid are respectively fed into the gas phase inlet and the liquid phase inlet, they are mixed inside the reactor and generate microbubbles.

[0079] The synthetic gas fermentation strain flow-addition system of this embodiment is used to form a fermentation system with an existing fermentation tank. When the yeast tank 1 of this embodiment is in operation, the yeast tank 1 receives the mature gas-eating bacteria strains after three-stage expansion, and continues to be cultured in the yeast tank 1. During the culture process, CO, deoxygenated water, nutrients and chemicals are introduced to provide a more ideal place for the reproduction of the gas-eating bacteria strains in the yeast tank 1. When the gas-eating bacteria strains are cultured and mature in the yeast tank 1, they are fed to the fermentation tank at a certain volume flow rate to continuously replenish the fermentation tank with fresh gas-eating bacteria strains.

[0080] The above description of the embodiments is to facilitate the understanding and use of the utility model by those skilled in the art. It is obvious that those familiar with the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the utility model is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the utility model without departing from the scope of the utility model should be within the scope of protection of the utility model.

Claims

1. A synthesis gas fermentation bacteria feed system, characterized in that: include: yeast jar; A three-stage bacterial strain expansion unit connected to the culture medium feed port of the yeast tank; A deoxygenated water unit connected to the upper part of the yeast tank; Respectively connect the nitrogen sealing pipeline unit and the tail gas treatment pipeline unit on the top of the yeast tank; A fermentation liquid circulation cooling unit connected to the bottom of the yeast tank to form a circulation; A CO gas inlet unit connected to the yeast tank; A chemical adding unit and a nutrient solution adding unit connected to the fermentation liquid circulation cooling unit; And a material conveying pipeline unit connected to the bottom of the yeast tank and outputting externally.

2. A synthesis gas fermentation bacteria feed system according to claim 1, characterized in that: The strain three-stage expansion culture unit comprises three strain expansion culture tanks connected in series, and the last strain expansion culture tank is also connected to the culture medium feed port through a culture medium feed pipe.

3. A synthesis gas fermentation bacteria feed system according to claim 1, characterized in that: The deoxygenated water unit comprises a water inlet arranged on the yeast tank and a deoxygenated water pipeline connected to the water inlet.

4. The synthesis gas fermentation bacteria feed system according to claim 1, characterized in that: The nitrogen sealing pipeline unit includes a nitrogen inlet arranged on the top of the yeast tank, and a nitrogen pipeline connected to the nitrogen inlet.

5. The synthesis gas fermentation bacteria feed system according to claim 1, characterized in that: The fermentation liquid circulation cooling unit comprises a circulation pipeline connected to the bottom of the yeast tank and forming a circulation, and a heat exchanger arranged on the circulation pipeline.

6. A synthesis gas fermentation bacteria feed system according to claim 5, characterized in that: The chemical adding unit comprises a chemical adding port arranged on the circulation pipeline, and a chemical pipeline connected to the chemical adding port; The nutrient solution adding unit comprises a nutrient solution adding port arranged on the circulation pipeline, and a nutrient solution pipeline connected to the nutrient solution adding port.

7. The synthesis gas fermentation bacteria feed system according to claim 1, characterized in that: The material conveying pipeline unit includes a discharge port arranged at the bottom of the yeast tank, a discharge pipeline connected to the discharge port, and a discharge pump arranged on the discharge pipeline; The CO gas inlet unit includes a CO gas inlet port arranged on the yeast tank, and a CO gas inlet pipeline connected to the CO gas inlet port.

8. The synthesis gas fermentation bacteria feed system according to claim 1, characterized in that: The volume of the yeast tank is 10-20% of that of the fermentation tank.

9. The synthesis gas fermentation bacteria feed system according to claim 1, characterized in that: The yeast tank is also provided with a biological reaction unit.

10. A fermentation assembly, characterized in that: The invention comprises a fermentation tank and a synthesis gas fermentation bacteria flow-addition system as claimed in any one of claims 1 to 9 connected to the fermentation tank.

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