Acquisition device for anaerobic culture and sequencing of microorganisms in tumor
By designing a microbial acquisition device with sequencing and culture acquisition module, insulation cylinder and core structure, the confusion and activity protection problems of microbial acquisition and preservation in the prior art are solved, and sampling accuracy and sample preservation effect are improved.
Patent Information
- Application Number
- CN202421326813.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-12
- Publication Date
- 2024-12-20
- Estimated Expiration
- 2034-06-12
AI Technical Summary
Existing microbial samplers are difficult to collect and preserve nucleic acids and microorganisms of microorganisms in tumors at the same time, and the samples are easily damaged during the collection process, especially in an anaerobic environment, which is difficult to effectively protect microbial activity.
A collection device including a sequencing acquisition module and a culture acquisition module was designed, and low-temperature refrigeration was carried out using visual and light-proof insulation cylinders. The core and T-shaped rods were used to achieve sealed sampling. The sampling needle bar structure improved the acquisition accuracy and passed The information slot stores QR codes to ensure data rigor.
It realizes the simultaneous collection and preservation of nucleic acids and microorganisms, protects sample activity, improves sampling accuracy and reliability, and solves the problems of small microbial mass and vulnerability of samples.
Smart Images
Figure CN222205239U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of microbial sampling instruments, in particular to a collection device for anaerobic culture and sequencing of intratumoral microorganisms. Background Art
[0002] With the in-depth study of host-microbe interactions, the concept of intratumoral microbiota present in tumor tissues has been proposed. Intratumoral microorganisms were first observed and described in the 19th century, but there was little progress in the following century. In recent years, with the development of detection technology and the deepening of understanding of the tumor microenvironment, more and more evidence has confirmed the existence of intratumoral bacteria, triggering more research in this field.
[0003] During the research process, microbial isolation and culture and high-throughput sequencing of microbial genes are two complementary studies on the microbiome. Traditional morphological identification methods can accurately identify some known strains and are still widely used for microbial identification. However, morphological identification methods are based on pure cultured flora, but some microorganisms in tumors are uncultured, which means that traditional morphological identification methods cannot identify these microorganisms and must be identified using high-throughput sequencing methods. The currently published microbial samplers have simple microbial collection modes, either only capable of collecting nucleic acids or only capable of collecting microorganisms. For the same sample, sampling confusion is easily caused. Moreover, since the biomass of most microorganisms in tumors is low, collection is difficult, and the collected samples cannot be stored at low temperatures in time, the death of microorganisms is easily caused, reducing the activity of microorganisms. In addition, microorganisms in tumors occasionally need to be sampled under anaerobic conditions. The current sampler uses a cotton swab for sampling and then puts it into a sampling tube. During operation, this method will expose the cotton swab to the external environment and come into contact with oxygen, thereby causing the death of anaerobic microorganisms in the tumor. Utility Model Content
[0004] In view of the shortcomings of the prior art described above, the purpose of the utility model is to provide a collection device for anaerobic culture and sequencing of intratumor microorganisms, which is used to solve the problems in the prior art that the sampler can only collect nucleic acids individually or the microorganisms are easily confused in the same sample, the low biomass is difficult to collect, and the collected samples cannot be stored at low temperatures in time, which may easily lead to the death of microorganisms.
[0005] To achieve the above-mentioned purpose and other related purposes, the utility model provides a collection device for anaerobic culture and sequencing of intratumor microorganisms, comprising: a collection mechanism, the collection mechanism comprising a sequencing collection module and a culture collection module, the sequencing collection module and the culture collection module are symmetrically arranged and fixedly connected, the sequencing collection module and the culture collection module have the same structure, the sequencing collection module comprises a cover, a first tube body, a second tube body, and a switch component for controlling the opening and closing of the first tube body, the first tube body is a tubular structure, the first tube body is located in the second tube body, the two ends of the second tube body are closed and a closed storage space is formed between the first tube body and the outer wall surface, the cover is detachably connected to the first end of the second tube body, the first end surface of the second tube body is provided with a feed channel connected to the storage space, the cover has an annular plunger matching the feed channel, the outer wall of the cover is provided with an information slot, and the inner wall of the first tube body is provided with a sliding component to control the connection state between the storage space and the inside of the first tube body;
[0006] A heat preservation mechanism, the heat preservation mechanism comprising a light-proof heat preservation cylinder and a visible heat preservation cylinder, the light-proof heat preservation cylinder is sleeved on the outer wall of the second tube body of the culture collection module, and the visible heat preservation cylinder is sleeved on the outer wall of the second tube body of the sequencing collection module;
[0007] Two telescopic sampling mechanisms are respectively arranged in the cover of the sequencing acquisition module and the cover of the culture acquisition module.
[0008] Optionally, the switch assembly includes a ball core and a T-shaped rod arranged in the first tube body, the ball core is located at the second end of the second tube body, the end of the vertical part of the T-shaped rod is fixedly mounted on the ball core, the vertical part of the T-shaped rod is rotatably mounted on the first tube body and the second tube body, and a first channel is provided on the ball core.
[0009] Optionally, the sliding components have two, and the two sliding components are symmetrically arranged along the central axis of the first tube body, each of the sliding components includes an L-shaped plate, a sliding column, a vertical plate and an elastic member, the vertical plate is parallel to the vertical part of the L-shaped plate and is fixedly connected to the horizontal part of the L-shaped plate, the sliding column is located between the vertical plate and the vertical part of the L-shaped plate, one end of the sliding column is fixedly connected to the horizontal part of the L-shaped plate, a sliding hole that slidably cooperates with the sliding column is provided on the inner wall of the first tube body, the vertical plate and the L-shaped plate are slidably cooperated with the inner wall of the first tube body and the outer wall of the first tube body respectively, and the elastic member controls the L-shaped plate to reset in the direction away from the sealing cover.
[0010] Optionally, the elastic member includes a spring, and the spring is sleeved on the sliding column.
[0011] Optionally, the telescopic sampling mechanism includes a telescopic component, a sampling needle, two fan-shaped baffles, and two control buttons for controlling the telescopic extension of the telescopic component, wherein the two fan-shaped baffles are fixedly mounted at the ends of the telescopic end of the telescopic component and are symmetrically arranged, and the two fan-shaped baffles are both slidably matched with the inner wall of the first tube body, and the fan-shaped baffles are colinear with the central axis of the first tube body. The sampling needle is fixedly connected to the end of the telescopic end of the telescopic component, and the sampling needle is provided with a plurality of barbs, and the plurality of barbs are all inclined toward the cover.
[0012] Optionally, the telescopic assembly includes an electric push rod.
[0013] Optionally, the detachable connection between the cover and the second tube body is a threaded connection, the cover has an internal thread, the outer wall of the second tube body has an external thread, the internal thread matches the external thread, the internal thread has a blank area from the end of the cover and / or the external thread has a blank area from the first end of the second tube body.
[0014] Optionally, the collection mechanism further comprises a bottom cover which is detachably covered on the second end of the second tube body.
[0015] As described above, the collection device for anaerobic culture and sequencing of intratumor microorganisms of the utility model has at least the following beneficial effects:
[0016] 1. Through the design of the sequencing collection module and the culture collection module, it is possible to collect nucleic acids and microorganisms and provide a suitable place for storing nucleic acids and microorganisms, thus solving the problem of confusion in the collection and storage of the same sample.
[0017] 2. Through the design of the information slot, the QR code can be pasted in the information slot. After the sampling is completed, it will be scanned by the computer and stored in the hard disk, ensuring the rigor of the sampling data.
[0018] 3. The visible insulation cylinder and the light-proof insulation cylinder can be detachably put on the outer wall of the second tube body of the sequencing acquisition module and the culture acquisition module respectively. They can be pre-cooled in advance when used and can be refrigerated at low temperature in a short time, so as to ensure the activity of the samples and allow them to be reused.
[0019] 4. By setting the ball core in the first tube body, when sampling, the ball core is placed close to the tumor surface and then the T-bar is rotated to drive the ball core to rotate, so that the first through hole is connected to the first tube body for sampling, thereby preventing the influence of the external environment as much as possible. After the sampling is completed, the T-bar is rotated to drive the ball core to rotate, so that the first through hole is not connected to the first tube body, thereby achieving sealing. The ball core seal has good sealing performance and can effectively reduce the influence of the external environment.
[0020] 5. The barbs on the sampling needle are tilted toward the capping direction, which ensures the amount of microorganisms collected, improves the accuracy and reliability of sampling, and solves the problem of difficulty in collecting small amounts of microorganisms. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 The display is a three-dimensional structural schematic diagram of the utility model;
[0022] Figure 2 Shown is a cross-sectional view of the present utility model;
[0023] Figure 3 Shown is an enlarged view of part A of the present utility model;
[0024] Figure 4 Shown is an exploded view of the sequencing acquisition module of the present utility model;
[0025] Figure 5 Shown is a three-dimensional structural schematic diagram of the sliding assembly of the utility model.
[0026] Component number description
[0027] Collection mechanism 1, sequencing collection module 11, culture collection module 12, cover 111, first tube body 112, second tube body 113, switch assembly 114, ball core 1141, T-bar 1142, first channel 1143, feed channel 115, annular plunger 116, information slot 117, sliding assembly 118, L-shaped plate 1181, sliding column 1182, vertical plate 1183, elastic member 1184, sliding hole 1185;
[0028] Insulation mechanism 2, light-proof insulation cylinder 21, visible insulation cylinder 22;
[0029] Telescopic sampling mechanism 3, telescopic assembly 31, sampling needle 32, fan-shaped baffle 33, control button 34, barb 35;
[0030] Internal thread 4, external thread 5, blank area 6, bottom cover 7. DETAILED DESCRIPTION
[0031] The following is a description of the implementation of the present invention by means of specific embodiments. People familiar with the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.
[0032] See also Figures 1 to 5. It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the utility model, so they have no substantial technical significance. Any modification of the structure, change in the proportional relationship, or adjustment of the size, without affecting the effects and purposes that can be achieved by the utility model, should still fall within the scope of the technical content disclosed by the utility model. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the utility model. Changes or adjustments in their relative relationships should also be regarded as the scope of the implementation of the utility model without substantially changing the technical content.
[0033] The following embodiments are only for illustration purposes and can be combined with each other, and are not limited to the contents presented in the following single embodiments.
[0034] In this embodiment, please refer to Figures 1 to 5The utility model provides a collection device for anaerobic culture and sequencing of intratumor microorganisms, comprising: a collection mechanism 1, a heat preservation mechanism 2 and two telescopic sampling mechanisms 3, the collection mechanism 1 comprises a sequencing collection module 11 and a culture collection module 12, the sequencing collection module 11 and the culture collection module 12 are symmetrically arranged and fixedly connected, the sequencing collection module 11 and the culture collection module 12 have the same structure, the sequencing collection module 11 comprises a cover 111, a first tube body 112, a second tube body 113, and a switch assembly 114 for controlling the opening and closing of the first tube body 112, the first tube body 112 is a tubular structure, the first tube body 112 is located in the second tube body 113, the first tube body 112 and the second tube body 113 are both made of transparent material, which is convenient for detection personnel to view the situation inside the first tube body 112, the second tube body 113 is closed at both ends and forms a gap with the outer wall of the first tube body 112 The sealing cap 111 is detachably connected to the first end of the second tube body 113. The first end face of the second tube body 113 is provided with a feed channel 115 connected to the storage space. The sealing cap 111 is provided with an annular plunger 116 matching the feed channel 115. When installed and used, deoxygenation operation is performed and filling is performed under anaerobic environment. Lysis solution and nucleic acid protection solution are loaded into the receiving space of the sequencing acquisition module 11, and microbial activity protection solution is loaded into the receiving space of the culture acquisition module 12. An information slot 117 is provided on the outer wall of the sealing cap 111. The design of the information slot 117 allows a QR code to be pasted in the information slot 117. After the sampling is completed, it is stored in the hard disk in the form of computer scanning, which ensures the rigor of the sampling data. A sliding component 118 is provided on the inner wall of the first tube body 112 to control the connection state between the storage space and the inside of the first tube body 112;
[0035] The insulation mechanism 2 includes a light-proof insulation cylinder 21 and a visible insulation cylinder 22. The light-proof insulation cylinder 21 is sleeved on the outer wall of the second tube body 113 of the culture collection module 12. The light-proof insulation barrel protects against light and ensures the activity of the microorganisms in the first tube body 112. The visible insulation cylinder 22 is sleeved on the outer wall of the second tube body 113 of the sequencing collection module 11. The visible insulation cylinder 22 is convenient for observing the lysis and preservation of the sample. The visible insulation cylinder 22 and the light-proof insulation cylinder 21 are respectively detachably sleeved on the outer walls of the second tube body 113 of the sequencing collection module 11 and the culture collection module 12. They are pre-cooled in advance when used and can be refrigerated in a short time, so that the activity of the sample can be guaranteed and it can be reused. The two telescopic sampling mechanisms 3 are respectively arranged in the cover 111 of the sequencing collection module 11 and the cover 111 of the culture collection module 12. The design of the sequencing collection module 11 and the culture collection module 12 allows the collection of nucleic acids and microorganisms and provides a suitable place for storing nucleic acids and microorganisms, thereby solving the problem of confusion in the collection and storage of the same sample.
[0036] In this embodiment, please refer to Figure 2 or Figure 4 The switch assembly 114 includes a ball core 1141 and a T-shaped rod 1142 disposed in the first tube body 112. The ball core 1141 is located at the second end of the second tube body 113. The end of the vertical part of the T-shaped rod 1142 is fixedly mounted on the ball core 1141. The vertical part of the T-shaped rod 1142 is rotatably mounted on the first tube body 112 and the second tube body 113. The ball core 1141 is provided with a first channel 1143. The ball core 1141 disposed in the first tube body 112 is placed close to the tumor surface during sampling and then the T-shaped rod 1142 is rotated to drive the ball core 1141 to rotate, so that the first through hole is connected to the first tube body 112 for sampling, thereby preventing the influence of the external environment as much as possible. After the sampling is completed, the T-shaped rod 1142 is rotated to drive the ball core 1141 to rotate, so that the first through hole is not connected to the first tube body 112, thereby achieving sealing. The ball core 1141 has good sealing performance and can effectively reduce the influence of the external environment.
[0037] In this embodiment, please refer to Figure 3 and Figure 5The sliding assembly 118 has two parts, and the two sliding assemblies 118 are symmetrically arranged along the central axis of the first tube body 112. Each sliding assembly 118 includes an L-shaped plate 1181, a sliding column 1182, a vertical plate 1183 and an elastic member 1184. The vertical plate 1183 is parallel to the vertical part of the L-shaped plate 1181 and is fixedly connected to the horizontal part of the L-shaped plate 1181. The sliding column 1182 is located between the vertical plate 1183 and the L-shaped plate 1181. 1181, one end of the sliding column 1182 is fixedly connected to the horizontal part of the L-shaped plate 1181, the inner wall of the first tube body 112 is provided with a sliding hole 1185 that slides with the sliding column 1182, the vertical plate 1183 and the L-shaped plate 1181 slide with the inner wall of the first tube body 112 and the outer wall of the first tube body 112 respectively, and the elastic member 1184 controls the L-shaped plate 1181 to reset in a direction away from the cover 111. When not in use, the storage space is not connected to the inside of the first tube body 112. When working, the elastic member 1184 slides toward the cover 111, so that the elastic member 1184 is compressed, and the inside of the first tube body 112 is connected to the storage space, so that the lysis solution, nucleic acid protection solution or microbial activity protection solution in the storage space flows into the first tube body 112.
[0038] In this embodiment, please refer to Figure 3 and Figure 5 The elastic member 1184 includes a spring, and the spring is sleeved on the sliding column 1182. The spring can store energy during the deformation process, so that the L-shaped plate 1181 can return to its original position when not under stress, and keep the storage space in a closed state.
[0039] In this embodiment, please refer to Figure 4 The telescopic sampling mechanism 3 includes a telescopic component 31, a sampling needle 32, two fan-shaped baffles 33, and two control buttons 34 for controlling the telescopic component 31 to be telescoped. The two fan-shaped baffles 33 are fixedly mounted at the ends of the telescopic end of the telescopic component 31 and are symmetrically arranged. The two fan-shaped baffles 33 are slidably matched with the inner wall of the first tube body 112. The fan-shaped baffles 33 are colinear with the central axis of the first tube body 112. The sampling needle 32 is fixedly connected to the end of the telescopic end of the telescopic component 31. A plurality of barbs 35 are provided on the sampling needle 32, and the barbs 35 are inclined toward the cover 111. By inclining the barbs 35 on the sampling needle 32 toward the cover 111, the amount of microorganisms collected is guaranteed, the accuracy and reliability of sampling are improved, and the problem of difficulty in collecting small amounts of microorganisms is solved.
[0040] In this embodiment, please refer to Figure 4The telescopic assembly 31 includes an electric push rod, which can achieve precise control and stability of the sampling needle 32 during telescopic operation.
[0041] In this embodiment, please refer to Figure 4 , the cover 111 and the second tube 113 are detachably connected as a threaded connection, the cover 111 has an internal thread 4, the outer wall of the second tube 113 has an external thread 5, the internal thread 4 matches the external thread 5, the internal thread 4 has a blank area 6 away from the end of the cover 111 and / or the external thread 5 has a blank area 6 away from the first end of the second tube 113. Through the design of the blank area 6, when the cover 111 is installed, the blank area 6 can ensure that the fan-shaped baffle 33 crosses the horizontal part of the L-shaped plate 1181 without interference, so that the distance between the fan-shaped baffle 33 and the first end of the second tube 113 is greater than the distance between the horizontal part of the L-shaped plate 1181 and the first end of the second tube 113. After the sampling is completed, the sampling needle 32 moves from the fan-shaped baffle 33 to the cover 111, and then compresses the elastic member 1184, so that the storage space is connected to the inside of the first tube 112.
[0042] In this embodiment, please refer to Figure 4 The collecting mechanism 1 further comprises a bottom cover 7 which is detachably provided at the second end of the second tube body 113. The detachable connection of the bottom cover 7 is a threaded connection, and the bottom cover 7 provided at the second end of the second tube body 113 can better seal the first tube body 112, avoid accidental leakage during transportation and storage, enhance stability, and reduce tilting.
[0043] Working principle: deoxygenation operation is performed during use and filling is performed under anaerobic environment. When the sequencing acquisition module 11 is installed, lysis solution and nucleic acid protection solution are loaded into the receiving space of the sequencing acquisition module 11, and the visible heat preservation cylinder 22 is inserted from the first end of the second tube body 113 into the outer wall of the second tube body 113 of the sequencing acquisition module 11. The telescopic end of the telescopic assembly 31 can be extended by a certain distance through the control button 34, so that the fan-shaped baffle 33 and the horizontal part of the L-shaped plate 1181 do not interfere with each other, and then the cover 111 is connected with the second tube body 113 so that the fan-shaped baffle 33 is at a distance from the second tube body 113. The distance between one end and the other end is greater than the distance between the horizontal part of the L-shaped plate 1181 and the first end of the second tube body 113; when installing the culture collection module 12, the microorganism activity protection liquid is filled into the receiving space of the culture collection module 12, and the light-proof heat preservation cylinder 21 is inserted from the first end of the second tube body 113 into the outer wall of the second tube body 113 of the culture collection module 12. The telescopic end of the telescopic assembly 31 can be extended by a certain distance through the control button 34, so that the fan-shaped baffle 33 and the horizontal part of the L-shaped plate 1181 do not interfere with each other, and then the cover 111 is connected with the second tube body 113 so that the fan-shaped baffle 3 3 is greater than the distance between the horizontal part of the L-shaped plate 1181 and the first end of the second tube body 113; when collecting, the bottom cover 7 is opened and close to the tumor surface, the T-shaped rod 1142 is rotated to drive the ball core 1141 to rotate, the first channel 1143 is connected with the first tube body 112, and the telescopic component 31 is controlled to extend by a control button 34, so that the sampling needle 32 fixedly connected to the telescopic end of the telescopic component 31 passes through the tumor surface and enters the tumor, and the plurality of barbs 35 of the sampling needle 32 penetrate into the tissue, and then the telescopic component 31 is controlled to retract by another control button 34, so that The sampling needle 32 is retracted into the first tube body 112 and then continues to shrink. The fan-shaped baffle 33 contacts the horizontal part of the L-shaped plate 1181 to slide the L-shaped plate 1181 toward the sealing cover 111, so that the elastic member 1184 is compressed, and the interior of the first tube body 112 is connected with the storage space, so that the lysis solution, nucleic acid protection solution or microbial activity protection solution in the storage space flows into the first tube body 112, and then the T-shaped rod 1142 is rotated so that the first channel 1143 is not connected with the first tube body 112, so that the first tube body 112 is in a closed state, and finally the bottom cover 7 is put on the second end of the second tube body 113.
[0044] In summary, the utility model can collect nucleic acids and microorganisms and provide a suitable place for storing nucleic acids and microorganisms through the design of the sequencing collection module 11 and the culture collection module 12, thereby solving the problem of confusion in the collection and storage of the same sample. Through the design of the information slot 117, a QR code can be pasted in the information slot 117, and after the sampling is completed, it is stored in the hard disk in the form of computer scanning, which ensures the rigor of the sampling data. The visible insulation cylinder 22 and the light-proof insulation cylinder 21 are detachably mounted on the outer wall of the second tube body 113 of the sequencing collection module 11 and the culture collection module 12, respectively. They are pre-cooled in advance when used, and can be refrigerated in a short time, so that the activity of the sample can be guaranteed and it can be reused. By setting the ball core 1141 in the first tube body 112, when sampling, close to the tumor surface and then rotating the T-bar 1142 to drive the ball core 1141 to rotate, so that the first through hole is connected to the first tube body 112 for sampling, thereby preventing the influence of the external environment as much as possible. After the sampling is completed, the T-bar 1142 is rotated to drive the ball core 1141 to rotate, so that the first through hole is not connected to the first tube body 112, thereby achieving sealing. The ball core 1141 seal has good sealing performance and can effectively reduce the influence of the external environment. The several barbs 35 on the sampling needle 32 are all tilted toward the cover 111, which ensures the collection amount of microorganisms, improves the accuracy and reliability of sampling, and solves the problem of difficulty in collecting small amounts of microorganisms. Therefore, the utility model effectively overcomes the various shortcomings of the prior art and has a high industrial utilization value.
[0045] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the technology may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed in the present invention shall still be covered by the claims of the present invention.
Claims
1. A collection device for anaerobic culture and sequencing of intratumoral microorganisms, characterized in that: include: A collection mechanism, the collection mechanism comprises a sequencing collection module and a culture collection module, the sequencing collection module and the culture collection module are symmetrically arranged and fixedly connected, the sequencing collection module and the culture collection module have the same structure, the sequencing collection module comprises a cover, a first tube body, a second tube body, and a switch component for controlling the opening and closing of the first tube body, the first tube body is a tubular structure, the first tube body is located in the second tube body, both ends of the second tube body are closed and a closed storage space is formed between the first tube body and the outer wall surface, the cover is detachably connected to the first end of the second tube body, the first end surface of the second tube body is provided with a feed channel connected to the storage space, the cover has an annular plunger matching the feed channel, the cover has an information slot on the outer wall, and the inner wall of the first tube body is provided with a sliding component to control the connection state between the storage space and the inside of the first tube body; A heat preservation mechanism, the heat preservation mechanism comprising a light-proof heat preservation cylinder and a visible heat preservation cylinder, the light-proof heat preservation cylinder is sleeved on the outer wall of the second tube body of the culture collection module, and the visible heat preservation cylinder is sleeved on the outer wall of the second tube body of the sequencing collection module; Two telescopic sampling mechanisms are respectively arranged in the cover of the sequencing acquisition module and the cover of the culture acquisition module.
2. The collection device for anaerobic culture and sequencing of intratumor microorganisms according to claim 1, characterized in that: The switch assembly includes a ball core and a T-shaped rod arranged in the first tube body, the ball core is located at the second end of the second tube body, the end of the vertical part of the T-shaped rod is fixedly installed on the ball core, the vertical part of the T-shaped rod is rotatably installed on the first tube body and the second tube body, and the ball core is provided with a first channel.
3. The collection device for anaerobic culture and sequencing of intratumor microorganisms according to claim 1, characterized in that: The sliding components have two, and the two sliding components are symmetrically arranged along the central axis of the first tube body. Each sliding component includes an L-shaped plate, a sliding column, a vertical plate and an elastic member. The vertical plate is parallel to the vertical part of the L-shaped plate and is fixedly connected to the horizontal part of the L-shaped plate. The sliding column is located between the vertical plate and the vertical part of the L-shaped plate. One end of the sliding column is fixedly connected to the horizontal part of the L-shaped plate. A sliding hole that slidably cooperates with the sliding column is provided on the inner wall of the first tube body. The vertical plate and the L-shaped plate are slidably cooperated with the inner wall of the first tube body and the outer wall of the first tube body respectively. The elastic member controls the L-shaped plate to reset in the direction away from the cover.
4. The collection device for anaerobic culture and sequencing of intratumoral microorganisms according to claim 3, characterized in that: The elastic member comprises a spring, and the spring is sleeved on the sliding column.
5. The collection device for anaerobic culture and sequencing of intratumor microorganisms according to claim 1, characterized in that: The telescopic sampling mechanism includes a telescopic component, a sampling needle, two fan-shaped baffles, and two control buttons for controlling the telescopic component to be extended and retracted, the two fan-shaped baffles are fixedly mounted at the ends of the telescopic end of the telescopic component and are symmetrically arranged, the two fan-shaped baffles are both slidably matched with the inner wall of the first tube body, the fan-shaped baffles are colinear with the central axis of the first tube body, the sampling needle is fixedly connected to the end of the telescopic end of the telescopic component, the sampling needle is provided with a plurality of barbs, and the plurality of barbs are all inclined toward the cover.
6. The collection device for anaerobic culture and sequencing of intratumoral microorganisms according to claim 5, characterized in that: The telescopic assembly includes an electric push rod.
7. The collection device for anaerobic culture and sequencing of intratumoral microorganisms according to claim 1, characterized in that: The detachable connection between the cover and the second tube body is a threaded connection, the cover has an internal thread, the outer wall of the second tube body has an external thread, the internal thread matches the external thread, the internal thread has a blank area away from the end of the cover and / or the external thread has a blank area away from the first end of the second tube body.
8. The collection device for anaerobic culture and sequencing of intratumor microorganisms according to claim 1, characterized in that: The collecting mechanism also includes a bottom cover which is detachably covered on the second end of the second tube body.