Intestinal flora regulation in-vitro simulation culture device
By using electric heating tubes and temperature sensors to simulate the internal temperature of the intestinal tract in the intestinal microbiota adjustment device, and using a raised structure made of silicone, the inaccurate temperature simulation and the intestinal structure in the prior art are solved. The problem of the inner wall improves the accuracy of the simulation results and the effect of the bacterial activity close to the actual situation.
Patent Information
- Application Number
- CN202420833678.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-22
- Publication Date
- 2024-12-20
- Estimated Expiration
- 2034-04-22
AI Technical Summary
The existing intestinal microbiota-regulated in vitro simulation culture device cannot effectively simulate the internal temperature of the intestinal tract, resulting in the impact of the activity of the microbiota, and the smooth surface of the culture structure cannot simulate the structure of the intestinal inner wall, affecting the accuracy of the simulation results.
A intestinal bacterial regulating in vitro simulation culture device was designed, using water embedded in the shell to achieve heat conduction, combining electric heating tubes and temperature sensors, logic controller adjusts the power of electric heating tubes to simulate the internal temperature of the intestinal tract, and uses silicone material Petri dishes simulate the inner wall of the intestine with a raised structure.
The temperature of the petri dish is closer to the internal temperature of the intestine, and the effect of bacterial activity is closer to the actual situation. At the same time, the simulation data is more in line with reality. The thermal conductivity and deformation ability of the silicone material make cleaning convenient.
Smart Images
Figure CN222205247U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of intestinal environment simulation, in particular to an intestinal flora regulation in vitro simulation culture device. Background Art
[0002] Intestinal flora is the normal microorganisms in the human intestine, such as bifidobacteria, lactobacilli, etc., which can synthesize a variety of vitamins necessary for human growth and development.
[0003] The existing referenceable Chinese utility model patent with announcement number CN217868807U discloses an in vitro simulated culture device for regulating intestinal flora. The second culture box can be moved in the inner cavity of the first culture box, so that the space in the inner cavity of the first culture box can be adjusted; the sliding cover rotates around the connecting rod and then inserted into the moving groove, so that the top of the first culture box is sealed; the first latch is inserted into the lock groove, so that the second culture box is fixed on the first culture box and cannot move; the second latch is set, so that the second latch moves and is inserted into the slide groove, so that the slide cover is limited and cannot move, which can avoid hitting the first culture box and causing unnecessary shaking of the slide cover; the sealing gasket is set, so that the sealing gasket contacts the slide cover, thereby increasing the sealing. The inner cavity space of the first culture box can be adjusted according to the use requirements, and when stored, the second culture box is fully inserted into the inner cavity of the first culture box, which can effectively reduce the occupied space.
[0004] Existing in vitro simulation devices can only control external temperature, while the bacterial culture structure cannot simulate the temperature inside the intestine. When there is a large deviation between the temperature and the temperature inside the intestine, the activity of the bacterial flora will be greatly affected, resulting in a large deviation between the simulation results and the actual results. At the same time, the surface of the bacterial culture structure of the existing simulation equipment is smooth and cannot simulate the structure of the intestinal wall, affecting the accuracy of the simulation results. Utility Model Content
[0005] 1. Technical issues to be solved
[0006] In view of the deficiencies in the prior art, the utility model provides an in vitro simulated culture device for regulating intestinal flora, which has the advantages of being able to adjust the temperature of the flora culture structure and to simulate the structure of the intestinal lining, thereby solving the above-mentioned technical problems.
[0007] (II) Technical solution
[0008] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an in vitro simulation culture device for regulating intestinal flora, comprising: a shell, a logic controller is fixedly installed on the left side of the shell, a side cover is movably installed on the left side of the shell, a limit block is embedded and installed on the top of the shell, a connecting plate is fixedly installed on the rear side of the limit block, a support rod is fixedly installed on the right side of the connecting plate, a culture dish is movably installed above the support rod, a top cover is movably installed on the top of the shell, a baffle is fixedly installed on the front side of the top cover, an electric heating tube is fixedly installed inside the shell, and a temperature sensor is fixedly installed inside the shell; the logic controller can control the power of the electric heating tube.
[0009] As a preferred technical solution of the present utility model, a rectangular recessed structure is provided on the right side of the top surface of the shell, a cavity engaged with the logic controller is provided on the left side of the shell, the shell is made of metal, and the side cover is movably connected to the shell by magnetic attraction; the shell can hold a certain amount of water for heat conduction.
[0010] As a preferred technical solution of the utility model, the front and rear sides of the shell are provided with an open hole structure that is engaged with the limit block, the limit block and the shell are slidably connected, and the left and right sides of the limit block are provided with a rectangular protrusion structure, and the rear side elevation of the rectangular protrusion structure of the limit block is in contact with the front side elevation of the shell; the block can limit the position of the connecting plate.
[0011] As a preferred technical solution of the utility model, the culture dish is a half-columnar structure, the culture dish is made of silica gel, and the interior of the culture dish is equidistantly provided with raised structures; the culture dish can simulate the internal structure of the intestine.
[0012] As a preferred technical solution of the utility model, the support rod is fixedly installed on the bottom surface and left and right sides of the rear side of the connecting plate at an angle of ninety degrees with the center of the culture dish as a reference, and the connecting plate is symmetrically installed on the front and back sides of the support rod with the center of the culture dish as a reference; the connecting plate can facilitate the connection between the support rod and the limit block.
[0013] As an optimal technical solution of the utility model, the top cover is made of transparent acrylic material, and a slide groove structure engaged with the limit block is provided below the top cover, so that a sliding connection is formed between the top cover and the limit block; the top cover can play a role in heat preservation and is convenient for observing the condition of the bacterial flora inside the culture dish.
[0014] As a preferred technical solution of the utility model, the electric heating tube is fixedly installed at the bottom of the rectangular recessed structure of the shell, and the temperature sensor is electrically connected to the logic controller; the temperature sensor can monitor the temperature of the water inside the shell.
[0015] Compared with the prior art, the utility model provides an in vitro simulated culture device for regulating intestinal flora, which has the following beneficial effects:
[0016] 1. The utility model is provided with a shell, and a rectangular recessed structure is provided on the top surface of the shell, which can be used to receive water. When the limit block slides to the bottom of the recessed structure of the shell, the bottom of the culture dish is located below the water surface. The water inside the shell is heated by the electric heating tube, and the heat is conducted to the inside of the culture dish through the water. The water temperature is monitored by the temperature sensor, and the temperature sensor can transmit the monitoring result to the logic controller. The logic controller can perform logical operations on the monitoring results and limit the power of the electric heating tube according to the operation results. The water temperature is limited by adjusting the set threshold of the logic controller. In this way, the temperature of the culture dish can be closer to the temperature inside the intestine, so that the activity of the flora outside the intestine is closer to the activity inside the intestine.
[0017] 2. The utility model sets a culture dish made of silicone. The interior of the culture dish is provided with a ridge structure for simulating the structure of the inner wall of the intestine. At the same time, the silicone material has good thermal conductivity and stable chemical properties. This method can ensure the chemical properties of the culture dish are stable while making the structure of the culture dish closer to the structure of the inner wall of the intestine, so that the simulation data is more in line with the actual data. At the same time, the silicone material has good deformation ability, which can facilitate the cleaning operation of the culture dish after completing the simulation experiment. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0019] Figure 2 This is a schematic diagram of the installation structure of the housing and the logic controller of the utility model;
[0020] Figure 3 This is a schematic diagram of the connection structure between the tooth culture dish and the connection plate of the utility model;
[0021] Figure 4 This is a schematic diagram of the cross-sectional structure of the shell of the utility model;
[0022] Among them: 1. outer shell; 11. logic controller; 12. side cover; 13. limit block; 14. connecting plate; 15. support rod; 16. culture dish; 17. top cover; 18. baffle; 19. electric heating tube; 110. temperature sensor. DETAILED DESCRIPTION
[0023] The following is a further detailed description of the implementation of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but cannot be used to limit the scope of the present invention.
[0024] In the description of the present invention, unless otherwise specified, "multiple" means two or more; the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third" and the like are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.
[0025] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0026] See also Figure 1 - Figure 4 In the present embodiment, an in vitro simulated culture device for regulating intestinal flora includes: a shell 1, a logic controller 11 is fixedly installed on the left side of the shell 1, a side cover 12 is movably installed on the left side of the shell 1, a limit block 13 is embedded and installed on the top of the shell 1, a connecting plate 14 is fixedly installed on the rear side of the limit block 13, a support rod 15 is fixedly installed on the right side of the connecting plate 14, a culture dish 16 is movably installed above the support rod 15, a top cover 17 is movably installed on the top of the shell 1, a baffle 18 is fixedly installed on the front side of the top cover 17, an electric heating pipe 19 is fixedly installed inside the shell 1, and a temperature sensor 110 is fixedly installed inside the shell 1.
[0027] A rectangular recessed structure is provided on the right side of the top surface of the shell 1, and a cavity engaged with the logic controller 11 is provided on the left side of the shell 1. The shell 1 is made of metal, and the side cover 12 is movably connected to the shell 1 by magnetic attraction.
[0028] The front and rear sides of the shell 1 are provided with an open hole structure that is engaged with the limit block 13. The limit block 13 and the shell 1 form a sliding connection. The left and right sides of the limit block 13 are provided with a rectangular protrusion structure. The rear side elevation of the rectangular protrusion structure of the limit block 13 contacts the front side elevation of the shell 1.
[0029] The culture dish 16 is a half-columnar structure, and is made of silica gel. Raised structures are evenly spaced inside the culture dish 16 .
[0030] The support rod 15 is fixedly installed on the bottom surface and left and right sides of the rear side of the connecting plate 14 at a 90-degree angle with the center of the culture dish 16 as the reference. The connecting plate 14 is symmetrically installed on the front and back sides of the support rod 15 with the center of the culture dish 16 as the reference.
[0031] The top cover 17 is made of transparent acrylic material. A slide groove structure engaged with the limit block 13 is provided below the top cover 17 , so that a sliding connection is formed between the top cover 17 and the limit block 13 .
[0032] The electric heating tube 19 is fixedly installed at the bottom of the rectangular recessed structure of the housing 1 , and an electrical connection is formed between the temperature sensor 110 and the logic controller 11 .
[0033] Specifically, the shell 1 can hold a certain amount of water for heat conduction, the model of the logic controller 11 is SR3B101BD, the logic controller 11 can control the power of the electric heating tube 19, the side cover 12 can protect the logic controller 11, the limit block 13 can limit the position of the connecting plate 14, the connecting plate 14 can facilitate the connection between the support rod 15 and the limit block 13, and limit the position of the culture dish 16, the top cover 17 can keep warm and facilitate the observation of the bacterial flora inside the culture dish 16, the baffle 18 can facilitate the movement of the top cover 17, the electric heating tube 19 can heat the water inside the shell 1, the model of the temperature sensor 110 is HBS-45, and the temperature sensor 110 can monitor the temperature of the water inside the shell 1.
[0034] When in use, a rectangular recessed structure is provided on the top surface of the shell 1, which can be used to receive water. When the limit block 13 slides to the bottom of the groove structure of the shell 1, the bottom of the culture dish 16 is located below the water surface. The water inside the shell 1 is heated by the electric heating tube 19, and the heat is conducted to the inside of the culture dish 16 through the water. The water temperature is monitored by the temperature sensor 110, and the temperature sensor 110 can transmit the monitoring result to the logic controller 11. The logic controller 11 can perform logical operations on the monitoring results and limit the power of the electric heating tube 19 according to the operation results. The water temperature is limited by adjusting the set threshold of the logic controller 11. This method can make the temperature of the culture dish 16 closer to the temperature inside the intestine. After the internal bacterial flora is inoculated, the top cover 17 needs to be installed on the top of the limit block 13 to limit the heat transfer and increase the temperature in the space between the bottom of the top cover 17 and the culture dish 16, so that the activity of the bacterial flora outside the intestine is closer to the activity inside the intestine. The culture dish 16 is made of silicone. The interior of the culture dish 16 is provided with a ridge structure to simulate the structure of the inner wall of the intestine. At the same time, the silicone material has good thermal conductivity and stable chemical properties. This method can ensure the chemical stability of the culture dish 16 while making the structure of the culture dish 16 closer to the structure of the inner wall of the intestine, so that the simulation data is more in line with the actual data. At the same time, the silicone material has good deformation ability, which can facilitate the cleaning operation of the culture dish 16 after the simulation experiment is completed.
[0035] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. An in vitro simulated culture device for regulating intestinal flora, characterized in that: include: A shell (1), a logic controller (11) is fixedly mounted on the left side of the shell (1), a side cover (12) is movably mounted on the left side of the shell (1), a limit block (13) is embedded and mounted on the top of the shell (1), a connecting plate (14) is fixedly mounted on the rear side of the limit block (13), a support rod (15) is fixedly mounted on the right side of the connecting plate (14), a culture dish (16) is movably mounted above the support rod (15), a top cover (17) is movably mounted on the top of the shell (1), a baffle (18) is fixedly mounted on the front side of the top cover (17), an electric heating pipe (19) is fixedly mounted inside the shell (1), and a temperature sensor (110) is fixedly mounted inside the shell (1).
2. The in vitro simulated culture device for regulating intestinal flora according to claim 1, characterized in that: A rectangular recessed structure is provided on the right side of the top surface of the shell (1), a cavity engaged with the logic controller (11) is provided on the left side of the shell (1), the shell (1) is made of metal, and the side cover (12) is movably connected to the shell (1) by means of magnetic attraction.
3. The in vitro simulated culture device for regulating intestinal flora according to claim 1, characterized in that: The front and rear sides of the housing (1) are provided with an open hole structure that engages with the limit block (13); the limit block (13) and the housing (1) are slidably connected; the left and right sides of the limit block (13) are provided with a rectangular protrusion structure; the rear side elevation of the rectangular protrusion structure of the limit block (13) is in contact with the front side elevation of the housing (1).
4. The in vitro simulated culture device for regulating intestinal flora according to claim 1, characterized in that: The culture dish (16) is a half-columnar structure, the culture dish (16) is made of silica gel, and ridge structures are arranged equidistantly inside the culture dish (16).
5. The in vitro simulated culture device for regulating intestinal flora according to claim 1, characterized in that: The support rod (15) is fixedly mounted on the bottom surface and left and right sides of the rear side of the connecting plate (14) at an angle of 90 degrees with the center of the culture dish (16) as a reference, and the connecting plate (14) is symmetrically mounted on the front and rear sides of the support rod (15) with the center of the culture dish (16) as a reference.
6. The in vitro simulated culture device for regulating intestinal flora according to claim 1, characterized in that: The top cover (17) is made of a transparent acrylic material. A sliding groove structure engaged with the limit block (13) is provided below the top cover (17), and a sliding connection is formed between the top cover (17) and the limit block (13).
7. The in vitro simulated culture device for regulating intestinal flora according to claim 2, characterized in that: The electric heating pipe (19) is fixedly mounted on the bottom of the rectangular recessed structure of the housing (1), and an electrical connection is established between the temperature sensor (110) and the logic controller (11).