Automatic cell multiplication culture equipment
By introducing driver components and interface components into the automatic cell amplification culture device, the problems of high equipment cost and complex operation are solved, and the efficient and low cost of cell culture is achieved, which is suitable for the promotion of cell culture.
Patent Information
- Application Number
- CN202420738880.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-11
- Publication Date
- 2024-12-20
- Estimated Expiration
- 2034-04-11
AI Technical Summary
The existing automatic cell amplification and culture equipment has high cost and complex operation, inconvenient transfer of culture medium, and difficult to regulate the culture environment, resulting in low culture efficiency and high cost.
A cell automatic expansion and culture device including a base and a driving component is designed. The transmission connection between the active plate and the driven plate is driven to shake the culture bed to ensure uniform mixing of the culture medium, and the culture environment is adjusted through the interface component to facilitate cell transfer and Culture fluid management.
It realizes stable fixation of the equipment, improves cell growth efficiency, reduces operation difficulty and cost, is highly applicable and suitable for promotion.
Smart Images
Figure CN222205264U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of cell culture, in particular to an automatic cell amplification and culture device. Background Art
[0002] Automatic cell expansion culture equipment is a device used to automatically culture and expand cells in vitro. It aims to achieve automated and efficient cell expansion, including cell inoculation, culture, monitoring and harvesting, thereby greatly simplifying the cell culture process and improving the quality of cell culture. It is often used in scientific research and the production of various cell products, such as vaccines, biological drugs, etc.
[0003] However, existing automatic cell expansion culture equipment also has some limitations, such as high equipment cost and high technical requirements for operators. At the same time, after the cells are cultured to a certain scale, the culture medium in the culture equipment is inconvenient to transfer, and the existing equipment is not convenient for real-time adjustment of the culture environment during the cell culture process, resulting in low culture efficiency and high culture cost.
[0004] Therefore, the present application designs a cell automatic expansion culture device to solve the above-mentioned technical problems. Utility Model Content
[0005] In order to solve the above technical problems, the utility model proposes an automatic cell proliferation and culture device.
[0006] To achieve the above object, the utility model provides a cell automatic expansion culture device, comprising a base, a driving component is arranged in the base, the driving component extends out of the base and is transmission-connected to a culture bed, and a culture bag for culture is arranged in the culture bed;
[0007] The driving assembly includes an active plate that slides longitudinally in the base, the top of the active plate extends out of the base and is transmission-connected to the end of the driven plate, the culture bed is fixedly connected to one end of the driven plate away from the active plate, and the middle of the driven plate is rotationally connected to the base;
[0008] The culture bag is provided with a first interface component, and the first interface component is connected with a second interface component provided on the culture bed;
[0009] A fixing component is arranged in the culture bed, and the fixing component is adapted to the culture bag and is detachably connected to the culture bag.
[0010] Preferably, the base is provided with a plurality of power cavities corresponding to the active plates, a cam is rotatably arranged in the power cavity, and an outer edge of the cam is in sliding contact with the bottom end of the active plate.
[0011] Preferably, guide grooves are provided on both sides of the power cavity, and guide blocks adapted to the guide grooves are fixedly connected to both sides of the active plate, and the guide blocks extend into the guide grooves and are slidably arranged with the guide grooves.
[0012] Preferably, a return spring in a stretched state is provided at the bottom end of the guide groove, and the return spring is fixedly connected to the bottom end of the guide block.
[0013] Preferably, the driven plate is provided with an annular transmission groove, a transmission block is slidably arranged in the transmission groove, and the transmission block extends out of the transmission groove and is fixedly connected to the active plate.
[0014] Preferably, the culture bed includes a bottom box fixedly connected to the end of the driven plate, a placement bed for placing culture bags is arranged in the bottom box, and the fixing assembly is arranged on the placement bed; a top cover for sealing the placement bed is hinged at one end of the bottom box.
[0015] Preferably, the fixing assembly comprises fixing grooves opened on both sides of the placement bed, a plurality of fixing bolts are slidably arranged in the fixing grooves, and threaded sections of the fixing bolts extend out of the fixing grooves and pass through the culture bag.
[0016] Preferably, the culture bag comprises a bag body, fixing strips corresponding to the fixing grooves are arranged on both sides of the bag body, fixing holes corresponding to the fixing bolts are arranged on the fixing strips, and the fixing bolts pass through the fixing holes and lock the bag body on the placement bed.
[0017] Preferably, the first interface assembly includes a plurality of first liquid ports and a plurality of first gas ports arranged on the bag body, and a one-way valve is respectively arranged in the first liquid port and the first gas port; the first liquid port and the first gas port are respectively connected to the second interface assembly.
[0018] Compared with the prior art, the utility model has the following advantages and technical effects: the utility model discloses an automatic cell expansion culture device, wherein the base serves as a supporting structure of the device and also as a counterweight to fix the device on the test bench and keep it stable, and the driving component can drive the culture bed to shake regularly, so that the culture material and cells in the culture bag therein are evenly mixed, the environment of the culture bag is kept stable, and the growth efficiency of the internal cells is guaranteed; the active plate moves up and down longitudinally on the base, and then drives the driven plate to shake through the transmission connection with the end of the driven plate, so that the culture bed containing the culture bag shakes, ensuring that the internal culture medium is evenly mixed; after the culture bag is fixed in the culture bed, the first interface component on it and the second interface component on the culture bed are connected together to adjust the culture environment in the culture bag, and at the same time, the internal cells can be easily transferred, which is convenient for cell transfer and continuous culture.
[0019] The utility model has a compact structure, strong applicability, and low equipment cost. It meets the technical requirements of operators and is convenient for real-time adjustment of the culture environment during the culture process, thereby improving the culture efficiency, reducing the culture cost, and is suitable for promotion. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] The drawings constituting a part of the present application are used to provide a further understanding of the present application. The illustrative embodiments and descriptions of the present application are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:
[0021] Figure 1 This is an axial view of the automatic cell expansion and culture device of the utility model;
[0022] Figure 2 For this utility model Figure 1 A partial enlarged view of middle A;
[0023] Figure 3 This is a schematic diagram of the side structure of the automatic cell proliferation and culture device of the utility model;
[0024] Figure 4 For this utility model Figure 3 A partial enlarged view of B in the middle;
[0025] Figure 5 This is a schematic diagram of the shaft view of the drive assembly of the utility model;
[0026] Figure 6 This is a schematic diagram of the structure of the drive assembly of the utility model;
[0027] In the figure: 1. base; 2. drive assembly; 3. culture bed; 4. culture bag; 5. active plate; 6. driven plate; 7. power chamber; 8. cam; 9. guide groove; 10. guide block; 11. reset spring; 12. transmission groove; 13. transmission block; 14. bottom box; 15. placement bed; 16. top cover; 17. fixing groove; 18. fixing bolt; 19. bag body; 20. fixing strip; 21. fixing hole; 22. first liquid port; 23. first gas port; 24. second liquid port; 25. second gas port; 26. handle; 27. limit groove; 28. limit block; 29. first connecting pipe; 30. second connecting pipe. DETAILED DESCRIPTION
[0028] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0029] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.
[0030] Reference Figure 1-Figure 6 As shown, this embodiment provides a cell automatic expansion culture device, including a base 1, a driving component 2 is arranged in the base 1, the driving component 2 extends out of the base 1 and is transmission-connected to a culture bed 3, and a culture bag 4 for culture is arranged in the culture bed 3;
[0031] The driving assembly 2 includes an active plate 5 which slides longitudinally in the base 1, the top of the active plate 5 extends out of the base 1 and is transmission-connected to the end of the driven plate 6, the culture bed 3 is fixed to the end of the driven plate 6 away from the active plate 5, and the middle of the driven plate 6 is rotationally connected to the base 1;
[0032] The culture bag 4 is provided with a first interface component, and the first interface component is connected with a second interface component provided on the culture bed 3;
[0033] A fixing component is arranged in the culture bed 3 , and the fixing component is matched with the culture bag 4 and is detachably connected to the culture bag 4 .
[0034] The utility model discloses a cell automatic expansion culture device, wherein a base 1 is used as a supporting structure of the device and as a counterweight to fix the device on a test bench and keep it stable, a driving component 2 can drive a culture bed 3 to shake regularly, so that the culture material and cells in the culture bag 4 are mixed evenly, the environment of the culture bag 4 is kept stable, and the growth efficiency of the internal cells is ensured; an active plate 5 is lifted and moved longitudinally on the base, and then the driven plate 6 is driven to shake through its transmission connection with the end of the driven plate 6, so that the culture bed 3 containing the culture bag 4 shakes, and the internal culture matrix is ensured to be mixed evenly; after the culture bag 4 is fixed in the culture bed 3, the first interface component on it and the second interface component on the culture bed 3 are connected together, the culture environment in the culture bag 4 is adjusted, and the internal cells can be conveniently transferred at the same time, so as to facilitate the transfer of cells and continuous culture. The utility model has a compact structure, strong applicability, and low equipment cost, and the technical ability of the operator is required, and it is convenient to adjust the culture environment of the culture process from time to time, which improves the culture efficiency and reduces the culture cost, and is suitable for promotion.
[0035] Furthermore, the culture bed 3 of this embodiment is provided with a sensor for monitoring the culture temperature and humidity, and the temperature and humidity in the culture bed 3 can be adjusted to maintain a stable culture environment.
[0036] In a further optimized solution, the base 1 is provided with a plurality of power chambers 7 corresponding to the active plates 5, and a cam 8 is rotatably provided in the power chamber 7, and the outer edge of the cam 8 is in sliding contact with the bottom end of the active plate 5. A motor for driving the cam 8 to rotate is provided in the base 1, and the cam 8 drives the active plate 5 to rise and fall in the power chamber 7 when rotating, providing power for the deflection of the driven plate 6; the speed, direction and start and stop of the motor can be controlled by a control panel provided on the base 1, so as to adjust the shaking speed of the culture bed 3.
[0037] Further optimization scheme, guide grooves 9 are provided on both sides of the power cavity 7, guide blocks 10 adapted to the guide grooves 9 are fixedly connected on both sides of the active plate 5, the guide blocks 10 extend into the guide grooves 9 and slide with the guide grooves 9; the bottom end of the guide grooves 9 is provided with a return spring 11 in a stretched state, and the return spring 11 is fixedly connected to the bottom end of the guide block 10. When the active plate 5 is raised or lowered, the guide block 10 slides in the guide groove 9, making the raising or lowering of the active plate 5 more stable; and when the active plate 5 drives the guide block 10 to rise, the return spring 11 is further stretched, and when the short shaft of the cam 8 contacts the bottom end of the active plate 5, the return spring 11 pulls the guide block 10 down to achieve reset.
[0038] Furthermore, a long strip-shaped limiting groove 27 is provided below the active plate 5, and a limiting block 28 is provided in the power cavity 7. The limiting block 28 passes through the limiting groove 27 and is slidably arranged with the limiting groove 27, so that the bottom end of the active plate 5 is limited and no shaking occurs.
[0039] In a further optimized solution, a ring-shaped transmission groove 12 is provided on the driven plate 6, and a transmission block 13 is slidably provided in the transmission groove 12. The transmission block 13 extends out of the transmission groove 12 and is fixedly connected to the active plate 5. When the active plate 5 moves up and down, the transmission block 13 is driven to move up and down, so that the transmission block 13 slides in the transmission groove 12 provided on the driven plate 6. The transmission groove 12 is set in an elliptical shape, so when the transmission block 13 is raised and lowered, the bottom end of the driven plate 6 will shake left and right; and the driven plate 6 rotates on the base 1 through the rotating shaft, and the transmission groove 12 and the culture bed 3 are respectively arranged on both sides of the rotating shaft to form a lever structure, thereby driving the culture bed 3 to shake.
[0040] Further optimized solution, the culture bed 3 includes a bottom box 14 fixedly connected to the end of the driven plate 6, a placement bed 15 for placing the culture bag 4 is arranged in the bottom box 14, and the fixed component is arranged on the placement bed 15; a top cover 16 for covering the placement bed 15 is hinged at one end of the bottom box 14. The culture bed 3 is made of a hinged bottom box 14 and a top cover 16, one end of which is hinged and the other end is detachable, which is convenient for opening and closing. During the culture process, the two are closed into a sealed structure to keep the internal culture environment constant; and the second interface component is arranged on both sides of the bottom box 14, which is convenient for external culture medium supplementation and waste liquid discharge; and the placement bed 15 is divided into two parts, one part is on the bottom box 14, and the other part is on the top cover 16, and both are provided with corresponding arc grooves to provide a placement space for the culture bag 4; and the fixed component is arranged on the lower half of the placement bed 15 and fixed to improve stability.
[0041] Furthermore, a handle 26 is provided on the top cover 16 to facilitate opening and closing of the top cover 16 .
[0042] In a further optimized solution, the fixing assembly includes fixing grooves 17 provided on both sides of the placement bed 15, and a plurality of fixing bolts 18 are slidably arranged in the fixing grooves 17, and the threaded sections of the fixing bolts 18 extend out of the fixing grooves 17 and pass through the culture bag 4. The fixing bolts 18 in the fixing grooves 17 can slide and adjust the position, and when the culture bag 4 is placed on the placement bed 15, the fixing bolts 18 pass through the culture bag 4 and are locked and fixed by nuts, and tightening the nuts can also lock and fix the fixing bolts 18 in the fixing grooves 17, so that the culture bag 4 cannot move and is prevented from deviating from the position when shaking.
[0043] In a further optimized solution, the culture bag 4 includes a bag body 19, and fixing bars 20 corresponding to the fixing grooves 17 are arranged on both sides of the bag body 19, and fixing holes 21 corresponding to the fixing bolts 18 are arranged on the fixing bars 20, and the fixing bolts 18 pass through the fixing holes 21 and lock the bag body 19 on the placement bed 15. The bag body 19 is formed into a cavity-type bag shape by laminating two high-separation material sheets around the periphery, and fixing bars 20 for reinforcing the bonding strength are arranged on both sides of the bag body 19, and the fixing holes 21 on the fixing bars 20 are adapted to the fixing bolts 18. When installed, the fixing bolts 18 pass through the fixing holes 21 to fix the culture bag 4.
[0044] In a further optimized solution, the first interface assembly includes a plurality of first liquid ports 22 and a plurality of first gas ports 23 arranged on the bag body 19, and a one-way valve is arranged in each of the first liquid ports 22 and the first gas ports 23; the first liquid ports 22 and the first gas ports 23 are respectively connected to the second interface assembly. The first liquid ports 22 are used to replenish the culture liquid and discharge the waste liquid in the culture bag 4, and are convenient for the transfer of cultured cells; and the first gas ports 23 are arranged to supply the mixed gas into the culture bag 4 and discharge the waste gas in the cell reproduction process, so as to facilitate the control of the culture environment in the culture bag 4.
[0045] Furthermore, the second interface assembly includes a second liquid port 24 and a second gas port 25 arranged on both sides of the bottom box 14. The second liquid port 24 is connected to the first liquid port 22 through a first connecting pipe 29, and the second gas port 25 is connected to the first gas port 23 through a second connecting pipe 30, thereby realizing the connection between the culture bag 4 and the outside world.
[0046] Furthermore, one-way valves are respectively arranged in the first liquid port 22 and the first gas port 23 according to the inlet and outlet directions to facilitate the inlet and outlet of liquid and gas and prevent backflow.
[0047] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.
[0048] The embodiments described above are only descriptions of the preferred methods of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should fall within the protection scope determined by the claims of the present invention.
Claims
1. A cell automatic expansion culture device, characterized in that: The invention comprises a base (1), wherein a driving assembly (2) is arranged inside the base (1), wherein the driving assembly (2) extends out of the base (1) and is drivingly connected to a culture bed (3), wherein a culture bag (4) for culture is arranged inside the culture bed (3); The driving assembly (2) comprises an active plate (5) which slides longitudinally in the base (1), the top end of the active plate (5) protruding from the base (1) and being transmission-connected to the end of a driven plate (6), the culture bed (3) being fixedly connected to an end of the driven plate (6) away from the active plate (5), and the middle part of the driven plate (6) being rotationally connected to the base (1); The culture bag (4) is provided with a first interface component, and the first interface component is connected to a second interface component provided on the culture bed (3); A fixing component is arranged in the culture bed (3), and the fixing component is adapted to the culture bag (4) and is detachably connected to the culture bag (4).
2. The cell automatic proliferation and culture device according to claim 1, characterized in that: The base (1) is provided with a plurality of power chambers (7) corresponding to the active plates (5), a cam (8) is rotatably arranged in the power chamber (7), and the outer edge of the cam (8) is in sliding contact with the bottom end of the active plate (5).
3. The cell automatic proliferation and culture device according to claim 2, characterized in that: Guide grooves (9) are arranged on both sides of the power chamber (7), and guide blocks (10) adapted to the guide grooves (9) are fixedly connected to both sides of the active plate (5), and the guide blocks (10) extend into the guide grooves (9) and are slidably arranged with the guide grooves (9).
4. The cell automatic expansion and culture device according to claim 3, characterized in that: A return spring (11) in a stretched state is arranged at the bottom end of the guide groove (9), and the return spring (11) is fixedly connected to the bottom end of the guide block (10).
5. The cell automatic proliferation and culture device according to claim 1, characterized in that: The driven plate (6) is provided with an annular transmission groove (12), a transmission block (13) is slidably arranged in the transmission groove (12), and the transmission block (13) extends out of the transmission groove (12) and is fixedly connected to the active plate (5).
6. The cell automatic proliferation and culture device according to claim 1, characterized in that: The culture bed (3) comprises a bottom box (14) fixedly connected to the end of the driven plate (6), a placement bed (15) for placing a culture bag (4) is arranged in the bottom box (14), and the fixing component is arranged on the placement bed (15); a top cover (16) for covering the placement bed (15) is hinged at one end of the bottom box (14).
7. The cell automatic expansion and culture device according to claim 6, characterized in that: The fixing assembly comprises fixing grooves (17) opened on both sides of the placement bed (15), a plurality of fixing bolts (18) are slidably arranged in the fixing grooves (17), and the threaded sections of the fixing bolts (18) extend out of the fixing grooves (17) and pass through the culture bag (4).
8. The cell automatic expansion and culture device according to claim 7, characterized in that: The culture bag (4) comprises a bag body (19), and fixing strips (20) corresponding to the fixing grooves (17) are arranged on both sides of the bag body (19), and fixing holes (21) corresponding to the fixing bolts (18) are arranged on the fixing strips (20), and the fixing bolts (18) pass through the fixing holes (21) and lock the bag body (19) on the placement bed (15).
9. The cell automatic expansion and culture device according to claim 8, characterized in that: The first interface component comprises a plurality of first liquid ports (22) and a plurality of first gas ports (23) arranged on the bag body (19); one-way valves are respectively arranged in the first liquid ports (22) and the first gas ports (23); and the first liquid ports (22) and the first gas ports (23) are respectively connected to the second interface component.