Cell co-culture weightlessness simulation experiment device

Abstract

The utility model discloses a cell co-culture simulated weightlessness experimental device, which relates to the technical field of cell culture and comprises a u-shaped support, a first rectangular frame rotationally arranged in the u-shaped support, a second rectangular frame rotationally arranged in the first rectangular frame, a first rectangular frame rotationally arranged in the u-shaped support, and a second rectangular frame rotationally arranged in the second rectangular frame. The first rectangular frame is arranged on the u-shaped support, the second rectangular frame is arranged on the u-shaped support, the first driving mechanism is arranged on the u-shaped support and drives the first rectangular frame to rotate, the second driving mechanism is arranged on the first rectangular frame and drives the second rectangular frame to rotate, the culture box is arranged in the second rectangular frame, and the sampling pipeline is arranged on the u-shaped support. The first right-angle pipe and the second right-angle pipe serve as a liquid pipeline and a rotating shaft of the first rectangular frame, the first communicating pipe and the second communicating pipe serve as a liquid pipeline and a rotating shaft of the second rectangular frame, and therefore sampling can be conducted from the culture box under the condition that rotation is not stopped.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell co-culture simulated weightlessness experimental device. Background Art

[0002] The cell co-culture simulated weightlessness experiment is a biomechanical experiment used to simulate the interaction between cells in a microgravity environment. This experimental device can be used to study the paracrine regulatory relationship between different cells under simulated weightlessness, as well as the co-culture of cells under normal gravity and simulated microgravity. The principle of the cell co-culture simulated weightlessness experiment is to rotate the cell culture device so that the cells cannot respond to gravity in a certain direction during the process of constantly changing direction, thereby simulating the microgravity biological effects of cells in space flight. This experimental method can not only be used to study the effects of microgravity on cell growth and function, but also to study the interactions and regulatory relationships between cells. By culturing a cell in a simulated weightlessness environment and then detecting the effects of its secretions on the function of another normally cultured cell, the interactions and regulatory mechanisms between cells in a microgravity environment can be further explored.

[0003] The experimental cycle of the cell co-culture simulated weightlessness experiment may take several days to weeks, depending on the experimental design and cell growth rate. During the experiment, it is necessary to regularly observe and test indicators such as cell growth and metabolites to evaluate the progress and effect of the experiment.

[0004] Existing equipment for cell co-culture simulated weightlessness experiments lacks the function of non-stop sampling. Testing of cell growth, metabolites and other indicators requires stopping rotation, which may affect the stability and reliability of the experimental results. Utility Model Content

[0005] In view of the deficiencies of the prior art, the utility model provides a cell co-culture simulated weightlessness experimental device, which solves the problems raised in the above background technology.

[0006] To achieve the above objectives, the utility model is implemented through the following technical solutions: a cell co-culture simulated weightlessness experimental device, comprising: a U-shaped bracket, a first rectangular frame rotatably arranged in the U-shaped bracket, a second rectangular frame rotatably arranged in the first rectangular frame, a first driving mechanism arranged on the U-shaped bracket to drive the first rectangular frame to rotate, a second driving mechanism arranged on the first rectangular frame to drive the second rectangular frame to rotate, a culture box arranged in the second rectangular frame, and a sampling pipeline arranged on the U-shaped bracket; the rotation direction of the first rectangular frame is perpendicular to the rotation direction of the second rectangular frame; the sampling pipeline comprises: a first vacuum cylinder, a three-way pipe connected to the first vacuum cylinder, a first rotating connector connected to the three-way pipe, a first right-angle connecting pipe connected to the first rotating connector and rotatably passing through the side wall of one end of the U-shaped bracket, and a first right-angle connecting pipe arranged at one end of the first rectangular frame and connected to the first right-angle connecting pipe A first connecting tube connected, a second rotating connector connected to the first connecting tube, a first connecting tube connected to the second rotating connector and rotatably passing through one end side wall of the first rectangular frame and fixedly connected to the second rectangular frame, a second connecting tube fixedly connected to the other end side wall of the second rectangular frame and rotatably passing through the other end of the first rectangular frame, a third rotating connector connected to the second connecting tube, a second connecting tube connected to the third rotating connector, a second right-angle connecting tube connected to the second connecting tube, the second right-angle connecting tube is fixed to the first rectangular frame and the other end of the second right-angle connecting tube can be rotatably passed through the U-shaped bracket, and also includes a fourth rotating connector connected to the second right-angle connecting tube, and a second vacuum cylinder connected to the fourth rotating connector; the first right-angle connecting tube is fixed to the first rectangular frame; the first connecting tube and the second connecting tube both lead to the culture box.

[0007] Preferably, a first support plate is fixedly provided on one side of the second rectangular frame, and a second support plate is fixedly provided on the other side of the second rectangular frame, the first support plate is provided with a first threaded column located at both ends of the culture box, the first threaded column is provided with a first pressure plate pressed by a first nut, the first pressure plate and the first support plate clamp one side of the culture box, the second support plate is provided with a second threaded column located at both ends of the culture box, the second threaded column is provided with a second pressure plate pressed by a second nut, and the second pressure plate and the second support plate clamp the other side of the culture box.

[0008] Preferably, through holes for connecting the first connecting tube and the second connecting tube are provided on both sides of the culture box, and a sealing cover is provided on the culture box.

[0009] Preferably, the first connecting tube and the second connecting tube have the same structure, and both include: an inner tube connected to the second rotating connector or the third rotating connector, a first sleeve fixed to the inner side of the second rectangular frame and sleeved on the inner tube, a second sleeve movably arranged in the first sleeve and sleeved on the inner tube, and a spring arranged in the second sleeve to push the second sleeve toward the culture box; a first ring with an outer diameter greater than the inner diameter of the first sleeve is provided on the second sleeve, one end of the spring is in contact with the second rectangular frame, and one end of the spring is in contact with the first ring; the inner tube includes: an axial tube section rotatably connected to the first rectangular frame and fixedly connected to the second rectangular frame, a bellows section connected to the axial tube section and located in the spring, a docking tube section passing through the first ring and docking with the through hole, the docking tube section being provided with a second ring with an outer diameter greater than the inner diameter of the first ring.

[0010] Preferably, the first driving mechanism includes: a first support frame fixedly arranged on one side of the U-shaped bracket, a first motor arranged on the first support frame, a first gear arranged on the output shaft of the first motor, and a second gear arranged on the second right-angle connecting tube and meshing with the first gear; the fourth rotating connector is located in the first support frame, and the second vacuum cylinder is fixed to the first support frame.

[0011] Preferably, the second driving mechanism includes: a second support frame arranged on one side of the first rectangular frame, a second motor arranged on the second support frame, a third gear arranged on the output shaft of the second motor, and a fourth gear arranged on the inner tube and meshing with the third gear; the first connecting tube or the second connecting tube is fixedly connected to the second support frame, and the second rotating connector or the third rotating connector is located in the second support frame.

[0012] Preferably, a third support frame is provided on the other side of the U-shaped bracket, the three-way pipe is fixedly connected to the third support frame, and the first rotating connector is located in the third support frame.

[0013] Compared with the prior art, the utility model has the following beneficial effects:

[0014] 1. The cell co-culture simulated weightlessness experimental device injects air through the second vacuum cylinder. The air enters the culture box from the second right-angle tube through the second connecting tube and then from the second connecting tube. The substances in the culture box enter the first connecting tube from the first connecting tube, pass through the first connecting tube, and finally flow out from the three-way pipe to be taken away for inspection. Sample sampling is convenient and quick. After sampling, the second vacuum cylinder can be used to push the substances back into the culture box, or it can not be pushed back.

[0015] 2. The first right-angle tube and the second right-angle tube of the cell co-culture simulated weightlessness experimental device are both liquid pipelines and the rotating axis of the first rectangular frame, and the first connecting tube and the second connecting tube are both liquid pipelines and the rotating axis of the second rectangular frame, so samples can be taken from the culture box without stopping rotation. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of the cell co-culture simulated weightlessness experimental device;

[0017] Figure 2 This is a schematic diagram of the cross-sectional structure of the cell co-culture simulated weightlessness experimental device;

[0018] Figure 3 It is a schematic diagram of the cross-sectional structure of the first connecting pipe or the second connecting pipe.

[0019] In the figure: U-shaped bracket 1, first rectangular frame 2, second rectangular frame 3, culture box 4, first air pump 5, three-way pipe 6, valve 7, first rotating connector 8, first right-angle connecting pipe 9, first connecting pipe 10, second rotating connector 11, first connecting pipe 12, second connecting pipe 13, third rotating connector 14, second connecting pipe 15, second right-angle connecting pipe 16, fourth rotating connector 17, second air pump 18, first support plate 19, second support plate 20, first threaded column 21, first Nut 22, first pressure plate 23, second threaded column 24, second nut 25, second pressure plate 26, through hole 27, sealing cover 28, inner tube 29, first sleeve 30, second sleeve 31, spring 32, first ring 33, shaft tube section 34, bellows section 35, docking tube section 36, second ring 37, first support frame 38, first motor 39, first gear 40, second gear 41, second support frame 42, second motor 43, third gear 44, fourth gear 45, third support frame 46. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0021] See also Figures 1 to 3The present embodiment provides a cell co-culture simulated weightlessness experimental device, comprising: a U-shaped bracket 1 as a base, a first rectangular frame 2 rotatably arranged in the U-shaped bracket 1, the first rectangular frame 2 being a first rotation direction, a second rectangular frame 3 rotatably arranged in the first rectangular frame 2, the second rectangular frame 3 being a second rotation direction, a first driving mechanism arranged on the U-shaped bracket 1 to drive the first rectangular frame 2 to rotate, a second driving mechanism arranged on the first rectangular frame 2 to drive the second rectangular frame 3 to rotate, a culture box 4 arranged in the second rectangular frame 3, the culture box 4 performs multi-cell culture; a sampling pipeline arranged on the U-shaped bracket 1; the rotation direction of the first rectangular frame 2 is perpendicular to the rotation direction of the second rectangular frame 3 Rotation direction; the sampling pipeline includes: a first vacuum cylinder 5, a three-way pipe 6 connected to the first vacuum cylinder 5, a valve 7 for sampling is provided on the three-way pipe 6, a first rotating connector 8 connected to the three-way pipe 6, a first right-angle connecting pipe 9 connected to the first rotating connector 8 and rotatably passing through the side wall of one end of the U-shaped bracket 1, the first right-angle connecting pipe 9 rotates with the first rectangular frame 2, and the first rotating connector 8 prevents the three-way pipe 6 from rotating with the first right-angle connecting pipe 9; a first connecting pipe 10 provided at one end of the first rectangular frame 2 and connected to the first right-angle connecting pipe 9, a second rotating connector 11 connected to the first connecting pipe 10, and a second rotating connector 11 connected to the second rotating connector 11 and A first connecting tube 12 rotatably passes through the side wall of one end of the first rectangular frame 2 and is fixedly connected to the second rectangular frame 3, and a second rotating connector 11 prevents the first connecting tube 10 from rotating with the first connecting tube 12, and the first connecting tube 12 is the rotation axis of the second rectangular frame 3 and the first rectangular frame 2; a second connecting tube 13 is fixedly connected to the side wall of the other end of the second rectangular frame 3 and rotatably passes through the other end of the first rectangular frame 2, and the second connecting tube 13 has the same function as the first connecting tube 12, a third rotating connector 14 connected to the second connecting tube 13, and a second connecting tube 15 connected to the third rotating connector 14, and the third rotating connector 14 prevents the second connecting tube 15 from rotating with the second connecting tube 1 3 rotates, a second right-angle connecting tube 16 connected to the second connecting tube 15, the second right-angle connecting tube 16 is fixed to the first rectangular frame 2 and the other end of the second right-angle connecting tube 16 can be rotated to pass through the U-shaped bracket 1, and the second right-angle connecting tube 16 has the same function as the first right-angle connecting tube 9; it also includes a fourth rotating connector 17 connected to the second right-angle connecting tube 16, and a second air pump 18 connected to the fourth rotating connector 17, and the fourth rotating connector 17 prevents the second air pump 18 from rotating with the second right-angle connecting tube 16; the first right-angle connecting tube 9 is fixed to the first rectangular frame 2; the first connecting tube 12 and the second connecting tube 13 both lead to the culture box 4. The first air pump 5 and the second air pump 18 can be syringes.When the valve 7 is closed, the second vacuum cylinder 18 injects air, and the air enters the second connecting pipe 15 from the second right-angle connecting pipe 16 and then enters the culture box 4 from the second connecting pipe 13, and the substances in the culture box 4 enter the first connecting pipe 10 from the first connecting pipe 12 and then pass through the first connecting pipe 10, and finally flow out from the three-way pipe 6 to be taken away for inspection; then the valve 7 is closed, and the second vacuum cylinder 18 is used to push the culture solution back to the culture box 4. Of course, the culture solution may not be pushed back, depending on the experimental situation.

[0022] A first support plate 19 is fixedly provided on one side of the second rectangular frame 3, and a second support plate 20 is fixedly provided on the other side of the second rectangular frame 3. The first support plate 19 is provided with first threaded columns 21 located at both ends of the culture box 4, and the first threaded columns 21 are provided with first pressing plates 23 pressed by first nuts 22. The first pressing plates 23 and the first support plate 19 clamp one side of the culture box 4. The second support plate 20 is provided with second threaded columns 24 located at both ends of the culture box 4, and the second threaded columns 24 are provided with second pressing plates 26 pressed by second nuts 25. The second pressing plates 26 and the second support plate 20 clamp the other side of the culture box 4. The culture box 4 is replaceable, which is convenient for conducting different cell co-culture experiments.

[0023] Through holes 27 for connecting the first connecting pipe 12 and the second connecting pipe 13 are provided on both sides of the culture box 4 , and a sealing cover 28 is provided on the culture box 4 .

[0024] The first connecting tube 12 and the second connecting tube 13 have the same structure and both include: an inner tube 29 connected to the second rotating connector 11 or the third rotating connector 14, a first sleeve 30 fixed to the inner side of the second rectangular frame 3 and sleeved on the inner tube 29, a second sleeve 31 movably arranged in the first sleeve 30 and sleeved on the inner tube 29, and a spring 32 arranged in the second sleeve 31 to push the second sleeve 31 toward the culture box 4; the second sleeve 31 is provided with a spring having an outer diameter larger than that of the first sleeve 30. The inner tube 29 comprises: an axial tube section 34 rotatably connected to the first rectangular frame 2 and fixedly connected to the second rectangular frame 3, a bellows section 35 connected to the axial tube section 34 and located in the spring 32, and a butt joint section 36 passing through the first circular ring 33 and butting against the through hole 27, wherein the butt joint section 36 is provided with a second circular ring 37 having an outer diameter greater than the inner diameter of the first circular ring 33. The shaft tube section 34 is the rotation axis between the second rectangular frame 3 and the first rectangular frame 2. When the culture box 4 is placed in the second rectangular frame 3, the docking tube section 36 is pushed back, the second sleeve 31 is retracted into the first sleeve 30 and the spring 32 is compressed, and the bellows section 35 allows the docking tube section 36 to be extended and retracted relative to the shaft tube section 34; after the docking tube section is released, the second sleeve 31 extends out under the action of elastic force and pushes the docking tube section into the through hole 27 to complete docking with the culture box 4, and a rubber gasket is arranged between the second ring 37 and the culture box 4.

[0025] The first driving mechanism includes: a first support frame 38 fixedly arranged on one side of the U-shaped bracket 1, a first motor 39 arranged on the first support frame 38, a first gear 40 arranged on the output shaft of the first motor 39, and a second gear 41 arranged on the second right-angle connecting tube 16 and meshing with the first gear 40; the fourth rotating connector 17 is located in the first support frame 38, and the second air pump 18 is fixed to the first support frame 38. The first support frame 38 supports the first motor 39 and the second air pump 18, and the first motor 39 drives the second right-angle connecting tube 16 to rotate through the second gear 41 and the first gear 40, thereby rotating the first rectangular frame 2, and the fourth rotating connector 17 prevents the second air pump 18 from rotating with the second right-angle connecting tube 16.

[0026] The second driving mechanism includes: a second support frame 42 provided at one side of the first rectangular frame 2, a second motor 43 provided on the second support frame 42, a third gear 44 provided on the output shaft of the second motor 43, and a fourth gear 45 provided on the inner tube 29 and meshing with the third gear 44; the first connecting tube 10 or the second connecting tube 15 is fixedly connected to the second support frame 42, and the second rotating connector 11 or the third rotating connector 14 is located in the second support frame 42. The second motor 43 drives the third gear 44 to rotate, the third gear 44 drives the fourth gear 45 to rotate, and the fourth gear 45 drives the inner tube 29 to rotate, thereby driving the second rectangular frame 3 to rotate.

[0027] A third support frame 46 is disposed on the other side of the U-shaped bracket 1 , the three-way pipe 6 is fixedly connected to the third support frame 46 , and the first rotary connector 8 is located in the third support frame 46 . The third support frame 46 fixes the three-way pipe 6 .

[0028] The cell co-culture simulated weightlessness experimental device is also provided with a controller of a first motor 39 and a point motor.

[0029] When the cell co-culture simulated weightlessness experimental device is used:

[0030] Place the cells and the culture fluid in the culture box 4, then place the culture box 4 in the second rectangular frame 3 and connect the first connecting tube 12 and the second connecting tube 13 to the culture box 4, and use the first pressing plate 23 and the second pressing plate 26 to fix the culture box 4; use the controller to start the first motor 39 and the second motor 43 to rotate the first rectangular frame 2 and the second rectangular frame 3; when sampling is required, use the second vacuum cylinder 18 to inject air, and the sample flows out of the three-way pipe 6 along the sampling pipeline for detection to predict the results of the cell co-culture simulated weightlessness experiment.

[0031] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A cell co-culture simulated weightlessness experimental device, characterized in that: include: A U-shaped bracket (1), a first rectangular frame (2) rotatably arranged in the U-shaped bracket (1), a second rectangular frame (3) rotatably arranged in the first rectangular frame (2), a first driving mechanism arranged on the U-shaped bracket (1) for driving the first rectangular frame (2) to rotate, a second driving mechanism arranged on the first rectangular frame (2) for driving the second rectangular frame (3) to rotate, a culture box (4) arranged in the second rectangular frame (3), and a sampling pipeline arranged on the U-shaped bracket (1); the rotation direction of the first rectangular frame (2) is perpendicular to the rotation direction of the second rectangular frame (2). The sampling pipeline comprises: a first air pump (5), a three-way pipe (6) connected to the first air pump (5), a first rotating connector (8) connected to the three-way pipe (6), a first right-angle connecting pipe (9) connected to the first rotating connector (8) and rotatably passing through a side wall of one end of the U-shaped bracket (1), a first connecting pipe (10) provided at one end of the first rectangular frame (2) and connected to the first right-angle connecting pipe (9), and a second rotating connector (11) connected to the first connecting pipe (10). a first connecting tube (12) connected to the second rotating connector (11) and rotatably passing through a side wall of one end of the first rectangular frame (2) and fixedly connected to the second rectangular frame (3); a second connecting tube (13) fixedly connected to the side wall of the other end of the second rectangular frame (3) and rotatably passing through the other end of the first rectangular frame (2); a third rotating connector (14) connected to the second connecting tube (13); a second connecting tube (15) connected to the third rotating connector (14); and a second connecting tube (15) connected to the second connecting tube (15). The invention also comprises a right-angle connecting tube (16), the second right-angle connecting tube (16) is fixed to the first rectangular frame (2) and the other end of the second right-angle connecting tube (16) can be rotated to pass through the U-shaped bracket (1), and also comprises a fourth rotating connector (17) connected to the second right-angle connecting tube (16), and a second vacuum cylinder (18) connected to the fourth rotating connector (17); the first right-angle connecting tube (9) is fixed to the first rectangular frame (2); the first connecting tube (12) and the second connecting tube (13) both lead to the inside of the culture box (4).

2. The cell co-culture simulated weightlessness experimental device according to claim 1, characterized in that: A first support plate (19) is fixedly provided on one side of the second rectangular frame (3), and a second support plate (20) is fixedly provided on the other side of the second rectangular frame (3); the first support plate (19) is provided with first threaded columns (21) located at two ends of the culture box (4); the first threaded columns (21) are provided with first pressing plates (23) pressed by first nuts (22); the first pressing plates (23) and the first support plate (19) clamp one side of the culture box (4); the second support plate (20) is provided with second threaded columns (24) located at two ends of the culture box (4); the second threaded columns (24) are provided with second pressing plates (26) pressed by second nuts (25); the second pressing plates (26) and the second support plate (20) clamp the other side of the culture box (4).

3. The cell co-culture simulated weightlessness experimental device according to claim 2, characterized in that: Through holes (27) for connecting the first connecting tube (12) and the second connecting tube (13) are provided on both sides of the culture box (4), and a sealing cover (28) is provided on the culture box (4).

4. The cell co-culture simulated weightlessness experimental device according to claim 3, characterized in that: The first connecting tube (12) and the second connecting tube (13) have the same structure, and both comprise: an inner tube (29) connected to the second rotating connector (11) or the third rotating connector (14), a first sleeve (30) fixed to the inner side of the second rectangular frame (3) and sleeved on the inner tube (29), a second sleeve (31) movably arranged in the first sleeve (30) and sleeved on the inner tube (29), and a spring (32) arranged in the second sleeve (31) for pushing the second sleeve (31) toward the culture box (4); the second sleeve (31) is provided with an inner sleeve (29) having an outer diameter greater than that of the first sleeve (30). The inner tube (29) comprises: an axial tube section (34) rotatably connected to the first rectangular frame (2) and fixedly connected to the second rectangular frame (3), a bellows section (35) connected to the axial tube section (34) and located inside the spring (32), and a butt joint section (36) passing through the first circular ring (33) and butting against the through hole (27); the butt joint section (36) is provided with a second circular ring (37) having an outer diameter greater than the inner diameter of the first circular ring (33).

5. The cell co-culture simulated weightlessness experimental device according to claim 4, characterized in that: The first driving mechanism comprises: a first support frame (38) fixedly arranged on one side of the U-shaped bracket (1), a first motor (39) arranged on the first support frame (38), a first gear (40) arranged on the output shaft of the first motor (39), and a second gear (41) arranged on the second right-angle connecting tube (16) and meshing with the first gear (40); the fourth rotating connector (17) is located in the first support frame (38), and the second vacuum cylinder (18) is fixed to the first support frame (38).

6. The cell co-culture simulated weightlessness experimental device according to claim 5, characterized in that: The second driving mechanism comprises: a second supporting frame (42) arranged on one side of the first rectangular frame (2), a second motor (43) arranged on the second supporting frame (42), a third gear (44) arranged on the output shaft of the second motor (43), and a fourth gear (45) arranged on the inner tube (29) and meshing with the third gear (44); the first connecting tube (10) or the second connecting tube (15) is fixedly connected to the second supporting frame (42), and the second rotating connector (11) or the third rotating connector (14) is located in the second supporting frame (42).

7. The cell co-culture simulated weightlessness experimental device according to claim 5, characterized in that: A third support frame (46) is provided on the other side of the U-shaped bracket (1), the three-way pipe (6) is fixedly connected to the third support frame (46), and the first rotating connector (8) is located inside the third support frame (46).

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