96-channel capillary electrophoresis analysis device

Abstract

The utility model discloses a 96-channel capillary electrophoresis analysis device which comprises a main machine, 96 capillary tubes are arranged on a mounting bracket through a fixing plate, and 96 capillary tube fixing holes are formed in the fixing plate; a fluorescence irradiation mechanism is arranged beside the mounting bracket; the capillary tube is introduced into the fluorescence irradiation mechanism from the mounting bracket; an irradiation light source assembly is arranged in front of the upper part of the fluorescence irradiation mechanism; two supporting plates with 96 through holes are arranged on the mounting bracket, one supporting plate is horizontally mounted, and the other supporting plate is vertically mounted; each capillary tube penetrates through the horizontally mounted support plate, then penetrates into the vertically mounted support plate, and is connected to the fluorescent irradiation mechanism from the vertically mounted support plate; a cooling fan is arranged at the position aligned with each capillary tube; and a collecting lens is arranged between the fluorescence irradiation mechanism and the irradiation light source assembly. Therefore, 96-channel full-load work can be realized, and the treatment efficiency is improved by several times.

Description

Technical Field

[0001] The utility model relates to the technical field of trace sample analysis and detection, in particular to a device for realizing trace sample detection through capillary electrophoresis. Background Art

[0002] Capillary electrophoresis is a commonly used chemical and biological molecular analysis method, which is widely used in the fields of drug analysis, environmental testing, bioengineering research, deoxyribonucleic acid, oligonucleotides, protein molecules, organic chemical component analysis, etc. The automatic capillary electrophoresis instrument includes an automatic injection system, a capillary, a sample detection system and a gel tank. The injection port and the sample outlet of the capillary are the negative and positive ends of the high-voltage power supply, respectively. The negatively charged sample migrates from the negative electrode (capillary injection port) to the positive electrode through the capillary under the high-voltage electric field. Since the migration speed of small molecules is faster than that of large molecules, different components will be separated due to different speeds during the migration process. Each component is detected and recorded when passing through the detector area to achieve the purpose of analysis and detection. However, although the existing multi-channel automated capillary electrophoresis device is provided with a 96-well plate, due to the constraints of many factors such as the capillary support structure, heat dissipation control, and fluorescence irradiation intensity, it is actually impossible to realize the simultaneous operation of 96 wells. Most of them only use 12 capillaries (i.e., one row), which wastes resources and leads to low processing efficiency. Utility Model Content

[0003] In view of the shortcomings of the prior art, the utility model provides a 96-channel capillary electrophoresis analysis device with a simple structure, a more reasonable design, and the ability to effectively solve the problems of capillary support, heat dissipation, fluorescence irradiation, etc. and a higher processing efficiency.

[0004] In order to solve the above technical problems, the utility model adopts the following technical solutions: a 96-channel capillary electrophoresis analysis device, comprising a host, a capillary is mounted on a mounting bracket through a fixing plate, the mounting bracket is mounted on the host, and 96 capillary fixing holes are arranged on the fixing plate; a fluorescent irradiation mechanism is arranged beside the mounting bracket, and the capillary is introduced into the fluorescent irradiation mechanism from the mounting bracket; an irradiation light source assembly is arranged in the upper front of the fluorescent irradiation mechanism, and the irradiation light source assembly is aligned with the window of the fluorescent irradiation mechanism, characterized in that: the capillary comprises 96 tubes, and each capillary fixing hole on the fixing plate corresponds to a capillary; two support plates with 96 through holes are arranged on the mounting bracket, and the two support plates are located on different sides of the mounting bracket, one of which is horizontally mounted and the other is vertically mounted; each capillary passes through the horizontally mounted support plate and then passes into the vertically mounted support plate, and is connected to the fluorescent irradiation mechanism from the vertically mounted support plate; a heat dissipation fan is arranged on the host at the position aligned with each capillary; a condenser is arranged between the fluorescent irradiation mechanism and the irradiation light source assembly.

[0005] Furthermore, an installation groove is provided at each end of the support plate, and the installation groove is a U-shaped groove; a fixing foot is provided on the installation bracket in the vertical direction, and a plug-in foot is provided in the horizontal direction. The vertical installation of a support plate is achieved by inserting the fixing foot into the upper and lower installation grooves of a support plate, and the horizontal installation of the support plate is achieved by plugging the front and rear installation grooves of another support plate into the two horizontally arranged plug-in feet.

[0006] Furthermore, the vertically mounted support plate is closer to the fluorescent irradiation mechanism than the horizontally mounted support plate, the vertically mounted support plate faces the fluorescent irradiation mechanism, and the two support plates are mounted on adjacent sides of the mounting bracket. The horizontally mounted support plate is located above one side edge of the fixed plate, and the main body of the vertically mounted support plate is higher than the horizontally mounted support plate, which is conducive to straightening out the capillaries and avoiding excessive twisting so as to more smoothly connect to the fluorescent irradiation mechanism.

[0007] Furthermore, the heat dissipation fan is installed beside the mounting bracket and outside the horizontally mounted support plate, and the heat dissipation fan is aimed at each capillary tube between the fixed plate and the horizontally mounted support plate. The capillaries at this position are basically evenly dispersed, so the heat dissipation effect at this position is better.

[0008] Preferably, the condenser is a condensing convex lens, which is installed on the main unit, and the light is condensed by the condensing convex lens and then irradiated into the window of the fluorescent irradiation mechanism.

[0009] The utility model is designed to have a support plate that can be used in conjunction with 96 capillaries to adapt to the bending angle of the 96 capillary bundles and provide adequate protection; at the same time, in response to the situation that the current will increase sharply when the electrophoresis current is applied to the 96 capillaries, resulting in a significant increase in heat generation, a cooling fan is arranged in front of the capillaries to specifically supply air to the capillaries to improve the heat dissipation performance and avoid the capillaries from breaking due to high temperature; in addition, in view of the high density of the 96 capillaries, a stronger light source is required to penetrate the capillaries and display the fluorescence on the CCD, so a convex lens is arranged behind the light source to focus the light to increase the light source intensity and focus on the capillary fluorescence illumination window. In this way, it is possible to achieve full load operation with 96 channels, and the processing efficiency is increased several times. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 This is the overall structure diagram of the utility model;

[0011] Figure 2 for Figure 1 The enlarged partial view shown;

[0012] Figure 3 A structural diagram showing a capillary tube mounted on a capillary tube mounting bracket;

[0013] Figure 4 This is the coordination structure diagram of the cooling fan and the capillary tube;

[0014] Figure 5 This is a structural diagram of the capillary passing through the fluorescent irradiation mechanism.

[0015] In the figure, 1 is the host, 2 is the mounting bracket, 21 is the fixing foot, 22 is the plug-in foot, 3 is the capillary, 31 is the fixing plate, 32 is the liquid filling plate, 4 is the cooling fan, 5 is the supporting plate, 51 is the mounting groove, 6 is the fluorescent irradiation mechanism, 7 is the irradiation light source assembly, and 8 is the focusing convex lens. DETAILED DESCRIPTION

[0016] In this embodiment, refer to Figure 1-Figure 5 The 96-channel capillary electrophoresis analysis device includes a host 1, a capillary 3 is mounted on a mounting bracket 2 through a fixing plate 31, the mounting bracket 2 is mounted on the host 1, and 96 capillary fixing holes are arranged on the fixing plate 31; a fluorescent irradiation mechanism 6 is arranged beside the mounting bracket 2, and the capillary 3 is introduced into the fluorescent irradiation mechanism 6 from the mounting bracket 2; an irradiation light source assembly 7 is arranged in front of the fluorescent irradiation mechanism 6, and the irradiation light source assembly 7 is aligned with the window of the fluorescent irradiation mechanism 6; the capillary 3 includes 96 pieces, and each capillary fixing hole on the fixing plate 31 corresponds to one piece. Capillaries 3, each capillary 3 goes downward into a liquid-filling plate 32 with 96 holes; two support plates 5 with 96 through holes are arranged on the mounting bracket 2, and the two support plates 5 are located on different sides of the mounting bracket 2, one of the support plates 5 is horizontally installed, and the other support plate 5 is vertically installed; each capillary 3 passes through the horizontally installed support plate 5 and then passes into the vertically installed support plate 5, and is connected to the fluorescent irradiation mechanism 6 from the vertically installed support plate 5; a cooling fan 4 is arranged on the main unit 1 to align with the position of each capillary 3; a condenser is arranged between the fluorescent irradiation mechanism 5 and the irradiation light source assembly 7.

[0017] A mounting groove 51 is provided at each end of the support plate 5, and the mounting groove 51 is a U-shaped groove; a fixing foot 21 is provided on the mounting bracket 2 in the vertical direction, and a plug-in foot 22 is provided in the horizontal direction. The vertical mounting of the support plate 5 is achieved by inserting the fixing foot 21 into the upper and lower mounting grooves 51 of a support plate 5, and the horizontal mounting of the support plate 5 is achieved by plugging the front and rear mounting grooves 51 of the other support plate 5 into the two horizontally arranged plug-in feet 22.

[0018] The vertically mounted support plate 5 is closer to the fluorescent irradiation mechanism 6 than the horizontally mounted support plate 5. The vertically mounted support plate 5 faces the fluorescent irradiation mechanism 6, and the two support plates 5 are mounted on adjacent sides of the mounting bracket 2. The horizontally mounted support plate 5 is located above one side edge of the fixing plate 31, and the main body of the vertically mounted support plate 5 is higher than the horizontally mounted support plate 5, which is conducive to straightening the capillaries 3 and avoiding excessive twisting so as to connect the fluorescent irradiation mechanism 6 more smoothly.

[0019] The heat dissipation fan 4 is installed beside (in front of) the mounting bracket 2 and outside the horizontally mounted support plate 5, and the heat dissipation fan 4 is aligned with each capillary tube 3 between the fixing plate 31 and the horizontally mounted support plate 5. The capillary tubes 3 at this position are in a state of being basically evenly dispersed, so the heat dissipation effect at this position is better.

[0020] The condenser is a condensing convex lens 8, which is installed on the host 1. The condensing convex lens 8 condenses the light and irradiates it into the window of the fluorescent irradiation mechanism 6, so that the fluorescence is displayed on the CCD.

[0021] The present invention has been described in detail above. The above description is only a preferred embodiment of the present invention and should not limit the scope of implementation of the present invention. That is, all equivalent changes and modifications made within the scope of this application should still fall within the scope of the present invention.

Claims

1. A 96-channel capillary electrophoresis analysis device, comprising a host, a capillary mounted on a mounting bracket through a fixing plate, the mounting bracket being mounted on the host, and 96 capillary fixing holes being arranged on the fixing plate; a fluorescent irradiation mechanism being arranged beside the mounting bracket, the capillary being introduced into the fluorescent irradiation mechanism from the mounting bracket; an irradiation light source assembly being arranged in front of the fluorescent irradiation mechanism, the irradiation light source assembly being aligned with a window of the fluorescent irradiation mechanism, characterized in that: The capillaries include 96 pieces, and each capillary fixing hole on the fixing plate corresponds to a capillary; two support plates with 96 through holes are arranged on the mounting bracket, and the two support plates are located on different sides of the mounting bracket, one of which is installed horizontally, and the other is installed vertically; each capillary passes through the horizontally installed support plate and then passes into the vertically installed support plate, and is connected to the fluorescent irradiation mechanism from the vertically installed support plate; a cooling fan is arranged on the main machine to align with the position of each capillary; a condenser is arranged between the fluorescent irradiation mechanism and the irradiation light source assembly.

2. The 96-channel capillary electrophoresis analysis device according to claim 1, characterized in that: A mounting groove is provided at each end of the support plate, and the mounting groove is a U-shaped groove; a fixing foot is provided on the mounting bracket in the vertical direction, and a plug-in foot is provided in the horizontal direction. The vertical mounting of a support plate is achieved by inserting the fixing foot into the upper and lower mounting grooves of a support plate, and the horizontal mounting of the support plate is achieved by plugging the front and rear mounting grooves of another support plate into the two horizontally arranged plug-in feet.

3. The 96-channel capillary electrophoresis analysis device according to claim 2, characterized in that: The vertically mounted support plate is closer to the fluorescent irradiation mechanism than the horizontally mounted support plate. The vertically mounted support plate faces the fluorescent irradiation mechanism. The two support plates are mounted on adjacent sides of the mounting bracket.

4. The 96-channel capillary electrophoresis analysis device according to claim 1, characterized in that: The horizontally installed support plate is located above one side edge of the fixed plate, and the main body of the vertically installed support plate is higher than the horizontally installed support plate.

5. The 96-channel capillary electrophoresis analysis device according to claim 4, characterized in that: The heat dissipation fan is installed beside the mounting bracket and is located outside the horizontally mounted support plate. The heat dissipation fan is aligned with each capillary tube between the fixing plate and the horizontally mounted support plate.

6. The 96-channel capillary electrophoresis analysis device according to claim 1, characterized in that: The condenser is a condensing convex lens, which is installed on the main machine. The condensing convex lens condenses the light and then irradiates it into the window of the fluorescent irradiation mechanism.

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