Microfluidic detector

Abstract

The utility model relates to the technical field of micro-fluidic instant detection, in particular to a micro-fluidic detector, which comprises an instrument body, the instrument body comprises a shell, a micro-fluidic chip is arranged in the shell, and the micro-fluidic detector is characterized in that a clamping component for clamping the micro-fluidic chip is arranged in the shell, and a clamping component for clamping the micro-fluidic chip is arranged in the clamping component. The clamping assembly comprises an upper clamping plate and a lower clamping plate which are hinged to each other, the upper clamping plate is provided with an exposure area for exposing the reaction bin of the substrate, and the upper clamping plate is correspondingly provided with an opening area which is communicated with the inlet and the outlet and is opposite to the detection bin at the periphery of the exposure area; the upper clamping plate is independently provided with a vibration module acting on the reaction bin and a detection module corresponding to the detection bin in the exposure area, and the lower end of the vibration module extends into the exposure area to be close to or in contact with the micro-fluidic chip. The microfluidic detector has the advantages of being small in size and convenient to install and use.

Description

Technical Field

[0001] The utility model relates to the field of microfluidic instant detection technology, and in particular to a microfluidic detector. Background Art

[0002] POCT refers to on-site sampling, on-site detection, and the use of portable and compact analytical instruments and supporting reagents to quickly obtain the target test object. As a result, microfluidic detection methods have gradually developed. Microfluidics refers to the technology of integrating a series of biochemical experimental processes into a chip, which has the advantages of small size and small injection volume. However, only one microfluidic chip cannot achieve both immune response and detection at the same time, and large instruments are still needed to detect the products of immune response.

[0003] In response to this problem, the Chinese utility model patent "A modular bioanalysis system based on droplet array chip and its application" with patent number CN201710978928.0 discloses a system that integrates the immune response and detection process into one system. The system includes an optical detection module and a reaction control module. The position of the optical detection module corresponds to the microfluidic chip detection pool and is used to emit and receive light signals. The reaction control module applies an external field to regulate the biochemical reaction in the reaction pool. The chip control module promotes the reaction in the reaction pool by driving the chip to reciprocate in a small amplitude and high frequency. The temperature control module controls the temperature of the microfluidic chip reaction pool and each channel. The system can realize full automation from sample loading to the end of detection.

[0004] However, the oscillation mode of the chip control module in the above-mentioned microfluidic system is similar to that of a mechanical shaker. The disadvantage is that a large space is required around the control module for the entire control module to drive the chip to shake, which is not conducive to the miniaturization of the instrument. The control module also needs to be shut down for maintenance after a long period of work. The large equipment and complex assembly relationship bring troubles to the maintenance of the control module. Utility Model Content

[0005] The technical problem to be solved by the utility model is to provide a microfluidic detector which occupies a small space and is convenient to install in view of the above-mentioned existing technical status.

[0006] The technical solution adopted by the utility model to solve the above technical problems is: the microfluidic detector includes an instrument body, the instrument body includes a shell, and the shell is provided with:

[0007] The microfluidic chip comprises a substrate, a reaction chamber is arranged on the substrate, an inlet for liquid inlet is opened upstream of the reaction chamber, a detection chamber for detection is connected downstream of the reaction chamber, and an outlet for liquid outlet is opened downstream of the detection chamber;

[0008] The invention is characterized in that: a clamping assembly for clamping the microfluidic chip is arranged in the shell, and the clamping assembly includes an upper clamping plate and a lower clamping plate which are hinged to each other, and an exposure area is opened on the upper clamping plate for exposing the reaction chamber of the substrate, and an opening area which is connected with the inlet and the outlet and opposite to the detection chamber is opened on the outer periphery of the exposure area corresponding to the upper clamping plate, and a vibration module acting on the reaction chamber and a detection module corresponding to the detection chamber are independently installed in the upper clamping plate in the exposure area, and the lower end of the vibration module extends into the exposure area to approach or contact the microfluidic chip.

[0009] In order to reduce the volume of the instrument body, preferably, the instrument body further includes a support seat for supporting the microfluidic chip, and the upper surface of the support seat is provided with an embedding port for embedding the clamping assembly, and the detection module includes:

[0010] An emission platform is arranged below the lower surface of the support seat, and an emission sensor opposite to the detection chamber is arranged on the emission platform, and the emission sensor serves as a light source and can adjust the wavelength of the light source;

[0011] The receiving platform is arranged on the upper surface of the support seat and is located above the detection chamber. The receiving platform is provided with a receiving sensor that cooperates with the transmitting sensor, and the receiving sensor is used to receive the optical signal emitted by the transmitting sensor and passing through the detection chamber. The embedded port design of the clamping component and the arrangement of the transmitting platform under the support seat fully utilize the space of the support seat, and integrate the microfluidic chip, the clamping component and the detection module into the support seat, thereby increasing the space utilization rate.

[0012] In order to improve the stability of the detection module, preferably, the cross section of the receiving platform is in the shape of a Chinese character "J", including two connecting legs connected to the upper surface of the support seat and a connecting section connecting the two connecting legs, the projection of the connecting section covers the detection chamber, the receiving sensor is arranged on the connecting section, and a through hole for the optical signal to pass through is opened on the connecting section. The design of the "J"-shaped cross section of the receiving platform increases the overall strength of the receiving platform.

[0013] In order to enable the connection base to be stably connected to the upper clamping plate, preferably, the vibration module is connected to the periphery of the exposed area of ​​the upper clamping plate through the connection base; the connection base includes:

[0014] A plug-in portion, the plug-in portion is located at the lower part of the connecting seat, the plug-in portion is circumferentially provided with a first mounting protrusion, and the first mounting protrusion is interference-fitted with the inner wall of the exposed area;

[0015] The mounting part is arranged on the plug-in part and connected to the vibration module. The plug-in part and the inner wall of the exposed area are interference-fitted so that the connecting seat can be stably arranged on the upper clamping plate to maintain a stable relative position.

[0016] In order to facilitate the user to control the distance between the vibration module and the reaction chamber during installation, preferably, the outer periphery of the connection between the installation part and the plug-in part protrudes outward to form a limiting platform. The limiting platform ensures that the distance between the vibration module and the reaction chamber is constant after installation, so that the mixing effect of the magnetic beads in the reaction chamber is stably achieved.

[0017] In order to stably connect the vibration module to the connection seat, preferably, the mounting portion and the plug-in portion are provided with a mounting channel connected to the exposed area, and the mounting channel is provided with a second mounting protrusion that is interference-fitted with the vibration module. The interference fit between the second mounting protrusion and the vibration module prevents the vibration module from shaking during operation, thereby ensuring the stability of the vibration frequency and position.

[0018] Furthermore, one end of the first mounting protrusion and the second mounting protrusion is respectively provided with a first mounting inclined surface and a second mounting inclined surface with cross sections increasing from small to large along the mounting direction.

[0019] In order to facilitate the user to control the installation depth of the vibration module, preferably, the vibration module includes:

[0020] A vibration element, which is located at the lower part of the vibration module and is used as a vibration source;

[0021] A fixing member is located on the upper part of the vibration module, the lower end of the fixing member is connected to the upper end of the vibration element, and the upper end of the fixing member protrudes circumferentially to form a first limiting portion, the outer diameter of the first limiting portion is larger than the inner diameter of the installation channel. The first limiting portion is set so that the upper end of the vibration module is clamped on the upper end of the connecting seat.

[0022] In order to limit the movement of the vibration module when the vibration element is working, preferably, the first limiting portion partially extends downward to form a second limiting portion, and correspondingly, a limiting groove capable of inserting the second limiting portion is recessed at the upper opening edge of the channel wall of the installation channel. The matching relationship between the second limiting portion and the limiting groove limits the circumferential rotation movement of the vibration module caused by vibration, thereby improving the stability of the vibration module when working.

[0023] Furthermore, the vibration element is a miniature eccentric vibration motor.

[0024] Compared with the prior art, the advantages of the utility model are: the exposure area in the microfluidic detector is independently installed with a vibration module acting on the reaction chamber, so that the user can quickly determine the position of the installed vibration module without considering the position distribution of multiple modules at the same time. The detection module and the vibration module are installed on the upper clamping plate at the same time, which reduces the volume of the instrument and makes the equipment miniaturized; the opening area of ​​the upper clamping plate is convenient for users to inject samples, discharge waste liquid and detect, and has the advantages of small size, easy installation and easy use. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the structure of an embodiment of the utility model;

[0026] Figure 2 A side view showing the positional relationship of the vibration module, the connection base, the clamping assembly, the support base, and the detection module of an embodiment of the utility model;

[0027] Figure 3 This is a schematic diagram of the structure of the detection module of an embodiment of the utility model;

[0028] Figure 4 It is a structural schematic diagram of the assembly relationship between the vibration module, the connecting seat and the clamping assembly of the embodiment of the utility model;

[0029] Figure 5 for Figure 4 A longitudinal section of

[0030] Figure 6 for Figure 4 Exploded diagram of the structure;

[0031] Figure 7 This is a schematic structural diagram of a connecting socket according to an embodiment of the utility model;

[0032] Figure 8 A longitudinal cross-sectional view of a connecting seat according to an embodiment of the utility model;

[0033] Fig. 9 A longitudinal cross-sectional view of the connecting base of the embodiment of the utility model at another angle;

[0034] Fig.10 It is a structural schematic diagram of a vibration module according to an embodiment of the utility model. DETAILED DESCRIPTION

[0035] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0036] like Figures 1 to 10The microfluidic detector of this embodiment is mainly used to obtain the concentration of the detected object by analyzing the changes in the optical signal generated by the biochemical reaction in the microfluidic chip. When the detection scene is outdoor field sampling detection, the microfluidic detector needs to provide users with a smaller volume for easy carrying, and can be repaired at any time to increase the flexibility of use. Therefore, it is beneficial to provide a microfluidic detector that occupies a small space and is easy to install. The structure of the microfluidic detector will be described below:

[0037] See also Figures 1 to 6 The microfluidic detector includes an instrument body, which includes a shell 1. A microfluidic chip 2 is arranged in the shell 1. The microfluidic chip 2 includes a substrate 21. A reaction chamber 22 is arranged on the substrate 21. An inlet 23 for liquid inlet is opened on the upstream of the reaction chamber 22. A detection chamber 24 for detection is connected to the downstream of the reaction chamber 22. An outlet 25 for liquid outlet is opened on the downstream of the detection chamber 24. A clamping assembly 3 for clamping the microfluidic chip 2 is arranged in the shell 1. The clamping assembly 3 includes mutually hinged The upper clamping plate 31 and the lower clamping plate 32 are respectively provided with an exposure area 311 for exposing the reaction chamber 22 of the substrate 21, and the upper clamping plate 31 is provided with an opening area 312 corresponding to the periphery of the exposure area 311, which is connected with the inlet 23 and the outlet 25 and is opposite to the detection chamber 24. The upper clamping plate 31 is independently installed with a vibration module 4 acting on the reaction chamber 22 and a detection module 5 corresponding to the detection chamber 24 in the exposure area 311, and the lower end of the vibration module 4 extends into the exposure area 311 to be close to or contact the microfluidic chip 2.

[0038] The instrument body of this embodiment also includes a support seat 6 for supporting the microfluidic chip 2, and the upper surface of the support seat 6 is provided with an embedding port 61 for the clamping assembly 3 to be embedded. The detection module 5 includes a launch platform 51 and a receiving platform 52, wherein the launch platform 51 is arranged below the lower surface of the support seat 6, and the launch platform 51 is provided with an emission sensor 511 opposite to the detection chamber 24, and the emission sensor 511 serves as a light source and can adjust the wavelength of the light source; the receiving platform 52 is arranged on the upper surface of the support seat 6 and above the detection chamber 24, and the receiving platform 52 is provided with a receiving sensor 521 that cooperates with the emission sensor 511, and the receiving sensor 521 is used to receive the light signal emitted by the emission sensor 511 and passing through the detection chamber 24. The embedding port design of the clamping assembly 3 and the launch platform 51 arranged below the support seat 6 make full use of the space of the support seat 6, and integrate the microfluidic chip 2, the clamping assembly 3 and the detection module 5 into the support seat 6, thereby increasing the space utilization rate and reducing the volume of the instrument body.

[0039] The receiving platform 52 of this embodiment has a "J"-shaped cross section, and includes two connecting legs 522 connected to the upper surface of the support seat 6 and a connecting section 523 connecting the two connecting legs 522. The projection of the connecting section 523 covers the detection chamber 24. The receiving sensor 521 is arranged on the connecting section 523, and a through hole 5231 for the light signal to pass through is opened on the connecting section 523. The "J"-shaped cross section design of the receiving platform 52 increases the overall strength of the receiving platform 52 and improves the stability of the detection module 5.

[0040] Please see Figure 7 The vibration module 4 of this embodiment is connected to the periphery of the exposed area 311 of the upper clamping plate 31 through the connecting seat 7; the connecting seat 7 includes a plug-in portion 71 and a mounting portion 72, wherein the plug-in portion 71 is located at the lower part of the connecting seat 7, and the plug-in portion 71 is provided with a first mounting protrusion 711 along the circumferential direction, and the first mounting protrusion 711 is interference-fitted with the inner wall of the exposed area 311; the mounting portion 72 is arranged on the plug-in portion 71 and connected to the vibration module 4. The interference fit between the plug-in portion 71 and the inner wall of the exposed area 311 enables the connecting seat 7 to be stably arranged on the upper clamping plate 31, and the relative position is kept stable.

[0041] Please see Figures 8 to 9 In this embodiment, the outer periphery of the connection between the mounting portion 72 and the plug-in portion 71 protrudes outward to form a limiting platform 73. The limiting platform 73 ensures that the distance between the vibration module 4 and the reaction chamber 22 is constant after installation, so that the mixing effect of the magnetic beads in the reaction chamber 22 is stably achieved, and it is convenient for users to control the distance between the vibration module 4 and the reaction chamber 22 during installation.

[0042] In this embodiment, the mounting portion 72 and the plug-in portion 71 are provided with a mounting channel 721 communicating with the exposed area 311, and the mounting channel 721 is provided with a second mounting protrusion 722 that is interference-fitted with the vibration module 4. The interference fit between the second mounting protrusion 722 and the vibration module 4 prevents the vibration module 4 from shaking during operation, so that the vibration module 4 is stably connected to the connecting seat 7, and the stability of the vibration frequency and position is ensured.

[0043] In this embodiment, one end of the first mounting protrusion 711 and the second mounting protrusion 722 is respectively provided with a first mounting inclined surface 712 and a second mounting inclined surface 723 with cross sections increasing from small to large along the mounting direction.

[0044] Please see Fig.10The vibration module 4 of this embodiment includes a vibration element 41 and a fixing member 42, wherein the vibration element 41 is used as a vibration source and is located at the lower part of the vibration module 4; the fixing member 42 is located at the upper part of the vibration module 4, the lower end of the fixing member 42 is connected to the upper end of the vibration element 41, and the upper end of the fixing member 42 protrudes circumferentially to form a first limiting portion 421, the outer diameter of the first limiting portion 421 is larger than the inner diameter of the installation channel 721. The setting of the first limiting portion 421 enables the upper end of the vibration module 4 to be clamped to the upper end of the connecting seat 7.

[0045] The first limiting portion 421 of this embodiment partially extends downward to form a second limiting portion 422, and a limiting groove 724 capable of inserting the second limiting portion 422 is correspondingly recessed at the upper opening edge of the channel wall of the installation channel 721. The matching relationship between the second limiting portion 422 and the limiting groove 724 limits the circumferential rotation movement of the vibration module 4 caused by vibration, limits the movement of the vibration module 4 when the vibration element 41 is working, and improves the stability of the vibration module 4 when working.

[0046] Finally, the vibration element 41 of this embodiment is a micro eccentric vibration motor.

[0047] The working principle of this microfluidic detector is:

[0048] 1) Workflow: add the sample solution and the reaction solution to be tested to the inlet 23 through the opening area 312, and after the reaction solution and the sample solution to be tested completely enter the reaction chamber 22, turn on the vibration module 4 for mixing, and then turn off the vibration module 4, and then add the color developing solution and the stop solution into the reaction chamber 22, turn on the vibration module 4 for mixing, and then obtain the solution to be tested; empty the solution to be tested in the reaction chamber 22 and push it into the detection chamber 24; after the solution to be tested completely enters the detection chamber 24, turn on the detection module 5 to perform absorbance detection on the solution to be tested, record the absorbance value, and the process ends.

[0049] 2) Installation process of vibration module 4: adjust the posture of vibration module 4 so that vibration element 41 is downward, mounting portion 72 of connecting seat 7 is upward, second limiting portion 422 is aligned with limiting groove 724, and inserted into mounting channel 721 of connecting seat 7, second limiting portion 422 is limitedly plugged into limiting groove 724, first limiting portion 421 is abutted against the upper end of mounting portion 72, second mounting protrusion 722 is tightly fitted with vibration module 4, and vibration module 4 and connecting seat 7 are installed; then adjust the posture of connecting seat 7 so that plug-in portion 71 is downward, insert downward into exposed area 311 until the lower surface of limiting platform 73 contacts with the upper surface of upper clamping plate 31, first mounting protrusion 711 is tightly fitted with plug-in portion 71, and fixture assembly 3 and vibration module 4 are installed.

Claims

1. A microfluidic detector, comprising an instrument body, the instrument body including a housing (1), and disposed within the housing (1) are: A microfluidic chip (2), including a substrate (21), a reaction chamber (22) is provided on the substrate (21), and an inlet (23) for liquid inlet is provided upstream of the reaction chamber (22), a detection chamber (24) for detection is communicated downstream of the reaction chamber (22), and an outlet (25) for liquid outlet is provided downstream of the detection chamber (24); It is characterized in that A clamping assembly (3) for clamping the microfluidic chip (2) is disposed within the housing (1), the clamping assembly (3) includes an upper clamping plate (31) and a lower clamping plate (32) that are hinged to each other, an exposure area (311) for exposing the reaction chamber (22) of the substrate (21) is provided on the upper clamping plate (31), an opening area (312) communicating with the inlet (23), the outlet (25) and opposite to the detection chamber (24) is correspondingly provided around the exposure area (311) of the upper clamping plate (31), a vibration module (4) acting on the reaction chamber (22) and a detection module (5) corresponding to the detection chamber (24) are independently installed in the exposure area (311) of the upper clamping plate (31), and the lower end of the vibration module (4) extends into the exposure area (311) to be close to or in contact with the microfluidic chip (2).

2. The microfluidic detector according to claim 1, characterized in that The instrument body further includes a support seat (6) for supporting the microfluidic chip (2), an embedding opening (61) for embedding the clamping assembly (3) is provided on the upper surface of the support seat (6), and the detection module (5) includes: A transmitting platform (51), disposed below the lower surface of the support seat (6), a transmitting sensor (511) opposite to the detection chamber (24) is provided on the transmitting platform (51), the transmitting sensor (511) serves as a light source and can adjust the wavelength of the light source; A receiving platform (52), disposed on the upper surface of the support seat (6) and above the detection chamber (24), a receiving sensor (521) cooperating with the transmitting sensor (511) is provided on the receiving platform (52), and the receiving sensor (521) is used to receive the optical signal emitted by the transmitting sensor (511) and passing through the detection chamber (24).

3. The microfluidic detector according to claim 2, characterized in that The cross-section of the receiving platform (52) is in a "ji" shape, including two connecting legs (522) connected to the upper surface of the support seat (6) and a connecting section (523) connecting the two connecting legs (522), the projection of the connecting section (523) covers the detection chamber (24), the receiving sensor (521) is provided on the connecting section (523), and a through hole (5231) for the optical signal to pass through is provided on the connecting section (523).

4. The microfluidic detector according to any one of claims 1 to 3, characterized in that The vibration module (4) is connected to the periphery of the exposure area (311) of the upper clamping plate (31) through a connecting seat (7); the connecting seat (7) includes: A plug-in portion (71), the plug-in portion (71) being located at the lower part of the connecting seat (7), the plug-in portion (71) being provided with a first mounting protrusion (711) along the circumferential direction, the first mounting protrusion (711) being interference-fitted with the inner wall of the exposed area (311); A mounting portion (72), the mounting portion (72) being arranged on the plug-in portion (71) and connected to the vibration module (4).

5. The microfluidic detector according to claim 4, characterized in that The outer periphery of the connection between the mounting portion (72) and the plug-in portion (71) protrudes outward to form a limiting platform (73).

6. The microfluidic detector according to claim 5, characterized in that The mounting portion (72) and the plug-in portion (71) are provided with a mounting channel (721) communicating with the exposed area (311) from top to bottom, and a second mounting protrusion (722) which is interference-fitted with the vibration module (4) is provided in the mounting channel (721).

7. The microfluidic detector according to claim 6, characterized in that One end of the first mounting protrusion (711) and the second mounting protrusion (722) is respectively provided with a first mounting inclined surface (712) and a second mounting inclined surface (723) whose cross sections increase from small to large along the mounting direction.

8. The microfluidic detector according to claim 6, characterized in that The vibration module (4) comprises: a vibration element (41), the vibration element (41) being located at the lower part of the vibration module (4) and being used as a vibration source; A fixing member (42), the fixing member (42) being located at the upper part of the vibration module (4), the lower end of the fixing member (42) being connected to the upper end of the vibration element (41), the upper end of the fixing member (42) being circumferentially protruding to form a first limiting portion (421), the outer diameter of the first limiting portion (421) being greater than the inner diameter of the installation channel (721).

9. The microfluidic detector according to claim 8, characterized in that The first limiting portion (421) partially extends downward to form a second limiting portion (422), and correspondingly, a limiting groove (724) capable of being inserted into the second limiting portion (422) is recessed at the upper opening edge of the channel wall of the installation channel (721).

10. The microfluidic detector according to claim 9, characterized in that: The vibration element (41) is a miniature eccentric vibration motor.

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