Microfluidic detector

Abstract

The utility model relates to a micro-fluidic detector, which comprises a detector body and is characterized in that the detector body comprises a shell, and a micro-fluidic chip reaction area, a reagent bag placing area and a scratching knife mechanism are arranged in the shell. The detection device disclosed by the utility model has the advantages that the microfluidic chip reaction area, the reagent bag placing area and the scratching knife mechanism are respectively and integrally arranged in the detection device, the reagent grooves for placing the reagent bags are communicated through the connecting channels, and the scratching of the reagent bags is realized under the operation that a user manually slides the scratching knife mechanism; the sample adding speed can be increased, the tedious problem caused by multiple times of sample adding is avoided, the size and the manufacturing cost of the detector can be reduced, and the method has important significance on real instant detection.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidic instant detection, in particular to a microfluidic chip detector capable of improving integration. Background Art

[0002] POCT refers to a method of sampling on site, testing results on site, and quickly obtaining the target test object using portable and compact analytical instruments and supporting reagents. For example, the Chinese utility model patent "A Portable Array Microfluidic Chip Device" of the existing patent application CN202220807148.6 (Announcement No. CN217410811U) provides a chip system that is quick, lightweight and compact. An image capture device, a sample loading device, a bracket and a sample loading control system are arranged above the microfluidic chip to enable rapid detection. However, there is only one inlet on the chip, so samples must be added to the corresponding inlets according to the order of the reactions. The operation is relatively cumbersome. In addition, at present, home testers have not yet appeared significantly in the public eye. Therefore, the market needs a disposable test device that is low-cost, fast and simple, can achieve precise control of the sample loading and detection process, is safe and reliable, and can be used without professional training. Utility Model Content

[0003] The technical problem to be solved by the utility model is to provide a detector which is easy to operate and can perform rapid home immune detection based on a microfluidic chip in response to the above-mentioned existing technical status.

[0004] The technical solution adopted by the utility model to solve the above technical problems is: the microfluidic detector includes an instrument body, characterized in that: the instrument body includes a shell, and the shell is provided with:

[0005] The reaction area of ​​the microfluidic chip includes a substrate and a mixing chamber arranged on the substrate, wherein at least two mixing chambers are arranged at intervals, each mixing chamber is connected to a reaction chamber downstream, each mixing chamber is provided with an inlet for liquid inlet upstream, and an outlet for liquid outlet is provided on the reaction chamber located at the most downstream;

[0006] Features:

[0007] a reagent package placement area, located on the substrate, comprising a base and reagent slots arranged in the base for placing reagent packages to be reacted, each reagent slot is connected through a connecting channel, the upstream of each connecting channel is connected to an upstream channel, and the ends of each connecting channel merge into a common confluence section, and the confluence section is fluidically connected to the inlet on the substrate; and

[0008] The cutting mechanism is located on the base, and comprises a cutting seat connected with the upstream channel, and a cutting knife which can slide along the cutting seat under the action of external force.

[0009] Furthermore, the cutting knife seat is in the form of a frame, and the inner wall surface of the frame is provided with a slide groove for the cutting knife to slide. Correspondingly, the cutting knife is provided with a cutting knife seat and a blade connected under the cutting knife seat.

[0010] Furthermore, a limiting groove engaged with the sliding groove is also provided on the inner wall surface of the frame, and the limiting groove can limit the sliding position of the cutting knife. Correspondingly, an elastic protrusion is convexly provided on the sliding rail of the cutting knife seat to cooperate with the limiting groove.

[0011] Furthermore, a seat body for accommodating the substrate and the base is provided in the shell, and the seat body is also respectively provided with a first socket and a second socket for the substrate and the base to be detachably inserted, and the shell is also provided with an insertion port for the cutting mechanism to be inserted, and the cutting seat is provided with a control part exposed outside the shell for controlling the blade.

[0012] Furthermore, an injection pump drive area is also provided in the shell, and the injection pump drive area includes a cavity of the injection pump, a water inlet pipe arranged on the cavity and connected to the outlet of the microfluidic chip reaction area, and a water outlet pipe arranged on the cavity. The liquid is discharged from the water outlet pipe through the reciprocating motion of the piston in the injection pump.

[0013] In order to facilitate the cleaning of the waste liquid collection chamber, preferably, a waste liquid collection area connected to the water outlet pipe is also provided in the shell, and the waste liquid collection area includes a waste liquid collection chamber. The shell is provided with a first opening corresponding to the waste liquid collection area, and the first opening can allow the waste liquid collection chamber of the waste liquid collection area to be removably inserted. Correspondingly, a handle exposed on the first opening is provided on the waste liquid collection chamber.

[0014] In order to improve the reaction speed and reaction accuracy in the reaction area of ​​the microfluidic chip, preferably, a micro-vibrator capable of enhancing mixing and a photoelectric detector and a photoelectric receiver capable of detecting reaction concentration are also provided in the shell on the reaction area of ​​the microfluidic chip.

[0015] Compared with the prior art, the utility model has the advantages that: by integrating the microfluidic chip reaction area, the reagent package placement area and the knife mechanism in the detector, the reagent slots where the reagent packages are placed are connected through connecting channels, and the reagent packages can be scratched by the user manually sliding the knife mechanism, which can increase the speed of sample addition and avoid the cumbersome problems caused by multiple sample additions, and can also reduce the size and cost of the detector, which is of great significance for realizing true instant detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of the detector in the embodiment of the utility model;

[0017] Figure 2 It is a partial cross-sectional view of the detector in the embodiment of the utility model;

[0018] Figure 3 It is a cross-sectional view of the detector in the embodiment of the utility model (blade omitted);

[0019] Figure 4 This is a schematic diagram of the structure of the microfluidic chip in the embodiment of the utility model;

[0020] Figure 5 This is a schematic diagram of the structure of the detector from another angle in the embodiment of the utility model;

[0021] Figure 6 for Figure 5 The structural schematic diagram of the shell is omitted;

[0022] Figure 7 A cross-sectional view of a local angle of the detector in the embodiment of the utility model;

[0023] Figure 8 It is a cross-sectional view of a part of the detector in the embodiment of the utility model at another angle. DETAILED DESCRIPTION

[0024] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0025] like Figures 1 to 8The microfluidic detector of this embodiment includes an instrument body 1, which includes a shell 11, and a microfluidic chip reaction area 2 is arranged in the shell 11, including a substrate 21 and a mixing chamber 22 arranged on the substrate 21, and at least two mixing chambers 22 are arranged at intervals, and each mixing chamber 22 is connected to a reaction chamber 23 downstream, and each mixing chamber 22 is provided with an inlet 24 for liquid inlet upstream, and an outlet 25 for liquid outlet is provided on the reaction chamber 23 located at the most downstream, and a reagent pack placement area 3 is arranged in the area above the base, and the reagent pack placement area 3 includes a base 31 and a reagent tank 32 arranged in the base 31 for placing the reagent pack to be reacted, and each reagent tank 32 is connected through a connecting channel 33, and each connecting channel 33 is connected to each other. The upstream of the connecting channels 33 is connected with an upstream channel 34, and the ends of the connecting channels 33 merge with a common confluence section 35, and the confluence section 35 is fluidically connected with the inlet 24 on the substrate 21; and a scratching knife 42 mechanism 4 is arranged in the area above the base 31, and the scratching knife 42 mechanism 4 includes a scratching knife seat 41 connected with the upstream channel 34, and a scratching knife 42 that can slide along the scratching knife seat 41 under the action of external force. By integrating the microfluidic chip reaction area 2, the reagent package placement area 3 and the scratching knife 42 mechanism 4 in the detector, the reagent tanks 32 where the reagent packages are placed are connected through the connecting channels 33, and the reagent packages are scratched when the user manually slides the scratching knife 42 mechanism 4, which can increase the speed of sample addition and avoid the cumbersome problems caused by multiple sample additions.

[0026] Specifically, the cutting knife seat 41 in this embodiment is in the form of a frame, and the inner wall surface of the frame is provided with a slide groove 43 for the cutting knife 42 to slide. Correspondingly, the cutting knife 42 is provided with a cutting knife seat 41 and a blade 44 connected under the cutting knife seat 41, and a limiting groove 46 engaged with the slide groove 43 is also provided on the inner wall surface of the frame. The limiting groove 46 can limit the sliding position of the cutting knife 42. Correspondingly, an elastic protrusion 47 that cooperates with the limiting groove 46 is convexly provided on the slide rail 45 of the cutting knife seat 41. In addition, a seat body 5 for accommodating the substrate 21 and the base 31 is also provided in the shell 11. The seat body 5 is also respectively provided with a first socket 12 and a second socket 13 for the substrate 21 and the base 31 to be detachably inserted, and an insertion port 24 for the cutting knife 42 mechanism 4 to be inserted is also opened on the shell 11. The cutting knife seat 41 is provided with a control part 48 exposed outside the shell 11 to control the blade 44. In this way, when the user pulls the operating part 48 exposed outside the shell 11, the cutting blade seat 41 can be pulled to slide together with the blade 44 to cut the reagent package.

[0027] Among them, an injection pump driving area 6 is also provided in the shell 11, and the injection pump driving area 6 includes a cavity 61 of the injection pump, an inlet pipe 62 arranged on the cavity 61 and connected to the outlet 25 of the microfluidic chip reaction area 2, and a water outlet pipe 63 arranged on the cavity 61. The liquid is discharged from the outlet pipe 63 through the reciprocating motion of the piston in the injection pump. In order to facilitate the cleaning of the waste liquid collection chamber 71, a waste liquid collection area 7 connected to the outlet pipe 63 is also provided in the shell 11. The waste liquid collection area 7 includes a waste liquid collection chamber 71. The shell 11 is provided with a first opening 14 corresponding to the waste liquid collection area 7. The first opening 14 can be removably inserted into the waste liquid collection chamber 71 of the waste liquid collection area 7. Correspondingly, a handle 72 exposed on the first opening 14 is provided on the waste liquid collection chamber 71. Finally, in order to improve the reaction speed and reaction accuracy in the microfluidic chip reaction area 2, a micro vibrator 8 capable of enhancing mixing and a photoelectric detector 9 and a photoelectric receiver 10 capable of detecting reaction concentration are also disposed on the microfluidic chip reaction area 2 in the housing 11.

[0028] At the same time, a magnet 20 is provided at the bottom of the reaction chamber 23, and the magnet 20 can adsorb the conjugate produced by the immune reaction into the reaction chamber 23. Generally, in immune reactions, reagent balls having capture reagent balls and labeling reagent balls are often used, wherein the capture reagent balls have magnetic beads coated with antibodies, antigens or secondary antibodies, and the labeling reagent balls are fluorescent microspheres coated with antibodies, antigens or secondary antibodies; the sample enters the mixing chamber 22 to dissolve the reagent balls to obtain a mixed sample, and the mixed sample enters the reaction chamber 23 to generate an immune reaction. At this time, the magnet 20 located at the bottom of the reaction chamber 23 adsorbs the conjugate of magnetic beads coated with antibodies, antigens or secondary antibodies produced by the immune reaction into the reaction chamber 23, and the reaction chamber 23 only contains the adsorbed magnetic bead conjugate.

[0029] In summary, by integrating the microfluidic chip reaction area 2, the reagent pack placement area 3 and the liquid supply mechanism in the detector, in addition, the microfluidic chip reaction area 2 and the reagent pack placement area 3 can be detachably inserted through their own sockets, so that rapid home immune detection based on the microfluidic chip can be conveniently performed. The detection process of the reagent pack in combination with the microfluidic chip is as follows (taking reagent packs No. 1, No. 2, No. 3 and No. 4 as examples):

[0030] ①, the substrate 21 corresponding to the reaction area 2 of the microfluidic chip is inserted from the first socket 12, and at the same time, the base 31 containing the No. 1, No. 2, No. 3 and No. 4 reagent packs is inserted from the second socket 13, and when the user holds the control part 48, the cutter seat 41 can be pulled to slide together with the blade 44 to cut the No. 1 reagent pack containing the reaction solution;

[0031] ② The reaction liquid can flow along the ends of the connecting channels 33 to the common confluence section 35 and enter the inlet 24 on the substrate 21 to contact with the sample;

[0032] ③. When you hear the beep, press the detection switch

[0033] ④. Trigger the micro vibrator 8 and vibrate and incubate for 5 minutes;

[0034] ⑤. Remove the micro vibrator 8, switch the electromagnet 20, and touch the bottom for 3 seconds;

[0035] ⑥. Cut open the No. 2 reagent pack and drain all the liquid inside the chip to the waste liquid collection area 7;

[0036] ⑦. Remove the electromagnet 20 and push in the micro vibrator 8;

[0037] ⑧. Scratch the No. 3 reagent pack and incubate for 5 minutes;

[0038] ⑨. Remove the micro vibrator 8, switch the electromagnet 20, and touch the bottom for 3 seconds;

[0039] ⑩. After cutting the No. 4 reagent pack, push all the liquids into the reaction chamber 23. The reaction chamber 23 detects the values ​​of different absorbances and displays the data on the LCD. At the same time, the mobile phone APP will pop up your test data results. The ability to conveniently perform rapid home immune testing based on microfluidic chips is one of the important technical means to achieve home health management in the future. It can also be associated with a smartphone APP account to achieve health data storage, real-time dynamic viewing of health data, establishment of personal health records, push of post-management intervention plans, and recommended diet and exercise plans.

Claims

1. A microfluidic detector, comprising an instrument body (1), characterized in that: The instrument body (1) comprises a housing (11), and the housing (11) is provided with: A microfluidic chip reaction zone (2), comprising a substrate (21) and a mixing chamber (22) arranged on the substrate (21), wherein at least two mixing chambers (22) are arranged at intervals, each mixing chamber (22) is connected to a reaction chamber (23) downstream, each mixing chamber (22) is provided with an inlet (24) for liquid inlet upstream, and an outlet (25) for liquid outlet is provided on the reaction chamber (23) located at the most downstream; Features: A reagent pack placement area (3), located on the substrate (21), comprising a base (31) and reagent slots (32) arranged in the base (31) for placing reagent packs to be reacted, each reagent slot (32) is connected via a connecting channel (33), the upstream of each connecting channel (33) is connected to an upstream channel (34), and the ends of each connecting channel (33) merge into a common confluence section (35), and the confluence section (35) is fluidically connected to an inlet (24) on the substrate (21); and The cutting mechanism (4) is located on the base (31), and comprises a cutting seat (41) connected to the upstream channel (34), and a cutting knife (42) that can slide along the cutting seat (41) under the action of external force.

2. The microfluidic detector according to claim 1, characterized in that: The cutting knife seat (41) is in the form of a frame, and the inner wall surface of the frame is provided with a slide groove (43) for the cutting knife (42) to slide. Correspondingly, the cutting knife (42) is provided with a cutting knife seat (41) and a blade (44) connected under the cutting knife seat (41).

3. The microfluidic detector according to claim 2, characterized in that: A limiting groove (46) engaged with the slide groove (43) is also provided on the inner wall surface of the frame body, and the limiting groove (46) can limit the sliding position of the cutting knife (42). Correspondingly, an elastic protrusion (47) that cooperates with the limiting groove (46) is convexly provided on the slide rail (45) of the cutting knife seat (41) 4. The microfluidic detector according to claim 3, characterized in that: The shell (11) is provided with a seat body (5) for accommodating the substrate (21) and the base (31), and the seat body (5) is also provided with a first socket (12) and a second socket (13) for the substrate (21) and the base (31) to be removably inserted, respectively. The shell (11) is also provided with an insertion port (24) for the cutting knife (42) mechanism (4) to be inserted, and the cutting knife seat (41) is provided with a control part (48) exposed outside the shell (11) for controlling the blade (44).

5. The microfluidic detector according to any one of claims 1 to 4, characterized in that: The housing (11) is also provided with a syringe pump drive area (6), the syringe pump drive area (6) comprising a syringe pump cavity (61), a water inlet pipe (62) provided on the cavity (61) and connected to an outlet (25) of a microfluidic chip reaction area (2), and a water outlet pipe (63) provided on the cavity (61), wherein the liquid is discharged from the water outlet pipe (63) by the reciprocating motion of the piston in the syringe pump.

6. The microfluidic detector according to claim 5, characterized in that: The shell (11) is also provided with a waste liquid collection area (7) connected to the water outlet pipe (63), the waste liquid collection area (7) includes a waste liquid collection chamber (71), the shell (11) is provided with a first opening (14) corresponding to the waste liquid collection area (7), the first opening (14) can be used for the waste liquid collection chamber (71) of the waste liquid collection area (7) to be removably inserted, and correspondingly, the waste liquid collection chamber (71) is provided with a handle (72) exposed on the first opening (14).

7. The microfluidic detector according to claim 6, characterized in that: A micro vibrator (8) capable of enhancing mixing, as well as a photoelectric detector (9) and a photoelectric receiver (10) capable of detecting reaction concentration are also arranged in the shell (11) on the microfluidic chip reaction area (2).

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