Auxiliary film sealing device for pcr (printed circuit board)

Abstract

The utility model discloses an auxiliary film sealing device for a pcr plate, which comprises a base, a pore plate seat, a driving device, a film pressing plate, a brushless motor, a storage battery, a control panel and a control button, the left side and the right side of the base are convexly provided with cross bars, and the pore plate seat for stably placing the pcr plate is arranged between the cross bars; the film pressing plate is arranged above the pore plate seat in a reciprocating motion manner through a driving device, and the overturning angle and the extrusion force of the film pressing plate are controlled through a brushless motor; a storage battery, a control panel and a control button are arranged on a left transverse bar of the base. The film sealing device adopts a semi-automatic design, the 96-pore plate seat is placed on the pore plate seat, the sealing film is placed on the pore plate seat, the button can be started, the film pressing plate is moved to the right side under the control of the control panel and then is put down until the film pressing plate is in contact and extruded with the sealing film, and finally the film pressing plate is controlled to move from right to left to complete the film pressing operation.

Description

Technical Field

[0001] The utility model belongs to the technical field of auxiliary tools for biological laboratories, and particularly relates to a PCR plate auxiliary film sealing device. Background Art

[0002] The sealing film is a transparent film that uses gel to seal the plate. It is widely used in experiments with 96-well plate holders, such as PCR, qPCR, ELISA, cell culture, long-term storage, automated workstation processing, and almost all other experiments. Its main function is to ensure that the sealing film fits closely with the 96-well plate holder to prevent liquid evaporation. Correct sealing ensures that the sealing film fits closely with the 96-well plate holder to prevent liquid evaporation, which can ensure the smoothness of the experiment.

[0003] The existing sealing method is mainly manual sealing, using a film pressing plate to slowly scrape and press the sealing film to make it completely sealed to the plate. The manual method is prone to the following shortcomings: 1. Uneven force, which can easily lead to loose adhesion or warping, or even bubbles, which will affect the final experimental results; 2. The operator's force is unstable, and small force can easily lead to loose adhesion and liquid evaporation; 3. Excessive force causes the sealing film to rub out burrs, reduce light transmittance, and affect experimental results; 4. The sealing film is not fully pressed, resulting in inconsistent adhesion in different areas, which will also affect experimental results. Therefore, an auxiliary tool needs to be designed for the sealing of the 96-well plate holder to meet the requirements of fast and correct sealing. Utility Model Content

[0004] Aiming at the deficiencies of the prior art, the utility model invents a PCR plate auxiliary film sealing device.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A PCR plate auxiliary film sealing device comprises a base, a perforated plate seat, a driving device, a film pressing plate, a brushless motor, a battery, a control panel and a control button. The left and right sides of the base are raised with horizontal bars, and a perforated plate seat for stably placing the PCR plate is arranged between the horizontal bars; the film pressing plate can be reciprocated by the driving device and is arranged above the perforated plate seat, and the film pressing plate controls the flip angle and the extrusion force by the brushless motor; the battery, the control panel and the control button are arranged on the horizontal bar on the left side of the base.

[0007] The film sealing device of the utility model adopts a semi-automatic design. The 96-well plate seat is placed on the well plate seat and the sealing film is placed. The film pressing plate can be started by pressing the button. Under the control of the control board, it moves to the right and then is lowered until it contacts and squeezes the sealing film. Finally, it is controlled to move from right to left to complete the film pressing operation.

[0008] Furthermore, the spacing between the left and right sides of the well plate seat and the left and right horizontal bars on the base is 1.0 mm to 2.0 mm, which is the thickness of the 96-well plate. The main purpose is to ensure that there is no gap left and right when the 96-well plate is placed.

[0009] Furthermore, the height of the well plate seat is 1.0mm to 2.0mm lower than the height of the horizontal bar, so that the upper surface of the 96-well plate is flush with the upper surface of the horizontal bar after being placed, ensuring that the film is scraped directly on the plane without any unevenness when the film pressing plate is scraping the film.

[0010] Furthermore, the driving device includes a movable frame, a screw rod, a sliding rod and a driving motor. The movable frame is arranged on the base for reciprocating movement through the screw rod and the sliding rod. The screw rod is provided with a driving motor. The function of the driving device is to control the reciprocating movement of the film pressing plate to the left and right.

[0011] Furthermore, the control button includes a switch button and a start button. The switch button is used to control the power of the film sealing device to turn on or off; the start button is used to control the operation of the film pressing plate. For example, after pressing the start button, the control panel first controls the film pressing plate to move to the right to a predetermined position through the drive device, and then the brushless motor controls the film pressing plate to flip and press down until it contacts the sealing film to generate a predetermined extrusion force. After that, the brushless motor controls the film pressing plate to stop flipping. Finally, the drive device controls the film pressing plate to move from right to left to complete the film pressing operation on the sealing film. After completion, the film pressing plate returns to its initial position.

[0012] The advantages of the utility model are: 1. The utility model is mainly designed to design a semi-automatic auxiliary device according to the sealing operation steps of 96-well plates, and the device is provided with a well plate seat for stably placing the 96-well plate, which is suitable for the stable placement of 96-well plates with no skirt, half skirt and full skirt; 2. The film pressing plate of the device is electrically controlled, and the flip angle of the film pressing plate and the squeezing force between the 96-well plate and the 96-well plate are controlled by a brushless motor, which can ensure that the force is consistent when scraping the film, and ensure that the sealing film and the 96-well plate are closely fitted to prevent liquid evaporation; 3. The front end of the film pressing plate is made of smooth material, which can apply greater pressure without deformation or damage, and the material is not easy to wear and will not scratch the surface of the sealing film; 4. The materials selected for the device are acid-resistant, alkali-resistant and corrosion-resistant, and can be disinfected in time after use to prevent cross contamination in the laboratory. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a stereoscopic diagram of a PCR plate auxiliary film sealing device of the utility model;

[0014] Figure 2 It is a top view of a PCR plate auxiliary film sealing device of the utility model;

[0015] Figure 3 It is a full-body diagram of a PCR plate auxiliary film sealing device of the utility model. DETAILED DESCRIPTION

[0016] The utility model is described in detail below with reference to the accompanying drawings:

[0017] In the description of the present utility model, it should be noted that the directions or positional relationships indicated by "up", "down", "left" and "right" are based on the directions or positional relationships shown in the accompanying drawings, or are the directions or positional relationships in which the utility model product is usually placed when in use. They are only for the convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, and a specific direction structure and operation, and therefore cannot be understood as a limitation on the present utility model.

[0018] like Figure 1-3 As shown, a PCR plate auxiliary sealing device includes a base 1, a well plate seat 2, a driving device 3, a film pressing plate 4, a brushless motor 5, a battery 6, a control board 7 and a control button 8. The left and right sides of the base 1 are raised with horizontal bars 9, and a well plate seat 2 for stably placing the PCR plate is arranged between the horizontal bars 9. The function of the two horizontal bars 9 and the well plate seat 2 is to provide a stable placement position for the 96-well plate, and the left and right side surfaces of the well plate seat 2 and the left and right horizontal bars 9 on the base 1 are 1.0mm~2.0mm apart. The height of the well plate seat 2 is 1.0mm~2.0mm lower than the height of the horizontal bars 9. This spacing and height difference is actually the thickness of the plastic made of the 96-well plate. The main purpose is to provide a stable placement "seat" for the 96-well plate to ensure that the 96-well plate will not be offset or pressed up during the film scraping process. The well plate seat 2 is provided with 96 wells matching with the 96-well plate, and in order to ensure the stable placement of the 96-well plate with no skirt, half skirt or full skirt, some structures gentle therewith can be designed as required.

[0019] The film pressing plate 4 can be reciprocated and arranged above the orifice plate seat 2 through the driving device 3. The film pressing plate 4 controls the flip angle and the extrusion force through the brushless motor 5. The width of the film pressing plate 4 is greater than the width of the 96-well plate, ensuring that the film pressing plate 4 can cross the entire 96-well plate after flipping and pressing down. The film pressing plate 4 is electronically controlled, so as to avoid the occurrence of uneven force, unstable force application, low film sealing efficiency, etc. when manually sealing the film. A battery 6, a control board 7 and a control button 8 are arranged on the horizontal bar 9 on the left side of the base 1. The battery 6 is a rechargeable battery, and a charging socket is arranged on the base 1; the control board 7 is the main control hub of the device electric control, and the control program can be written as needed; the control button 8 is divided into a switch button and a start button. The switch button controls the switch of the power supply, and the start button is used to control the activity of the film pressing plate 4 according to a pre-set program, such as controlling the leftmost end position, the rightmost end position, the moving speed or the flip angle of the film pressing plate 4, the extrusion force (controlled by the torque of the brushless motor), etc. of the driving device 3 in the program.

[0020] The driving device 3 includes a moving frame 31, a screw rod 32, a slide rod 33 and a driving motor 34. The moving frame 31 is reciprocated and arranged on the base 1 through the screw rod 32 and the slide rod 33. The screw rod 32 and the slide rod 33 are located on the left and right sides of the orifice plate seat 2. The driving motor 34 is arranged on the screw rod 32. When the driving motor 34 works, the moving frame 31 can be controlled to move left and right. The moving frame 31 spans the orifice plate seat 2, and the left and right ends of the film pressing plate 4 are rotatably connected to the moving frame 31. The brushless motor 5 is fixedly installed on the moving frame 31, and the main shaft of the brushless motor 5 is directly fixedly connected to the rotating shaft of the film pressing plate 4.

[0021] In this embodiment, a clamp may be further provided on the right horizontal bar 9, and the function of the clamp is to clamp the right end of the sealing film to ensure that the sealing film will not be scraped away when the film is scraped.

[0022] Although the specific embodiments of the present invention have been described and illustrated in detail above, it should be noted that various changes and modifications may be made to the above embodiments without departing from the spirit of the present invention and the scope of the appended claims.

Claims

1. A PCR plate auxiliary sealing device, characterized in that: The invention comprises a base (1), a perforated plate seat (2), a driving device (3), a film pressing plate (4), a brushless motor (5), a storage battery (6), a control panel (7) and a control button (8); horizontal bars (9) are provided on the left and right sides of the base (1); a perforated plate seat (2) for stably placing a PCR plate is provided between the horizontal bars (9); the film pressing plate (4) is reciprocally movable above the perforated plate seat (2) through the driving device (3); the film pressing plate (4) controls the flip angle and the extrusion force through the brushless motor (5); and a storage battery (6), a control panel (7) and a control button (8) are provided on the horizontal bar (9) on the left side of the base (1).

2. A PCR plate auxiliary sealing device according to claim 1, characterized in that: The distance between the left and right side surfaces of the orifice plate seat (2) and the left and right horizontal bars (9) on the base (1) is 1.0 mm to 2.0 mm.

3. A PCR plate auxiliary sealing device according to claim 2, characterized in that: The height of the orifice plate seat (2) is 1.0 mm to 2.0 mm lower than the height of the horizontal bar (9).

4. A PCR plate auxiliary sealing device according to claim 3, characterized in that: The driving device (3) comprises a moving frame (31), a screw rod (32), a sliding rod (33) and a driving motor (34); the moving frame (31) is arranged on the base (1) for reciprocating movement via the screw rod (32) and the sliding rod (33); and the driving motor (34) is arranged on the screw rod (32).

5. A PCR plate auxiliary sealing device according to claim 4, characterized in that: The control button (8) comprises a switch button and a start button.

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