PCR calibration device and PCR instrument

Abstract

The utility model relates to the technical field of PCR (Polymerase Chain Reaction) detection, and discloses a PCR calibration device, which is used for calibrating a PCR instrument with an excitation light source and a fluorescence receiving module and comprises a calibration body and a temperature measuring piece. The calibration body is used for being connected with a detection cavity of the PCR instrument in an inserted mode and comprises a main body part and a light guide part which are sequentially arranged in the length direction of the calibration body, the light guide part is used for transmission of an excitation light source and excitation fluorescence, a fluorescence cavity is formed in the light guide part and filled with fluorophor, and fluorescent substances are placed in the fluorescence cavity and used for fluorescence calibration. The temperature measuring piece is installed on the calibration body and used for calibrating the temperature of the PCR instrument. According to the PCR calibration device disclosed by the utility model, temperature calibration and fluorescence calibration can be simultaneously carried out, and the reliability of temperature calibration and fluorescence calibration is improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of PCR detection, and specifically relates to a PCR calibration device and a PCR instrument. Background Art

[0002] At present, fluorescence quantitative PCR instruments are widely used in medical testing, clinical diagnosis, in vitro diagnostic reagent research and development and manufacturing, entry-exit inspection and quarantine, and scientific research. Its core technology refers to adding fluorescent groups to the PCR reaction system, using the accumulation of fluorescent signals to monitor the entire PCR reaction process in real time, and finally using the standard curve to perform quantitative analysis on unknown templates. This mainly involves two parts: temperature and optics. The accuracy and reliability of temperature and optical physical parameters determine the accuracy and reliability of the detection results of the fluorescence quantitative PCR instrument.

[0003] However, the PCR adjustment and calibration system in the prior art generally separates temperature calibration and fluorescence calibration physically and operationally, and the two are independently calibrated in terms of performance. Multiple steps of calibration are required in operation, which is cumbersome and increases the complexity of the calibration system. In actual use, fluorescence calibration is generally completed at room temperature, and the impact of temperature differences on fluorescence calibration is not fully considered, which can easily lead to inaccurate calibration results and makes it impossible to perform temperature calibration and fluorescence calibration at the same time. Utility Model Content

[0004] The utility model aims to provide a PCR calibration device and a PCR instrument, which can perform temperature calibration and fluorescence calibration at the same time.

[0005] In order to achieve the above object, the utility model provides a PCR calibration device for calibrating a PCR instrument with an excitation light source and a fluorescence receiving module. The PCR calibration device comprises:

[0006] The calibration body is used to be plugged into the detection cavity of the PCR instrument. The calibration body includes a main body and a light guide portion which are sequentially arranged along the length direction of the calibration body. The light guide portion is used for the transmission of the excitation light source and the excitation fluorescence. The light guide portion is provided with a fluorescence cavity for filling the fluorescent body.

[0007] The temperature measuring component is installed on the calibration body and is used to calibrate the temperature of the PCR instrument.

[0008] In an embodiment of the utility model, a liquid injection channel connected to the fluorescent cavity is provided on the calibration body, the liquid injection channel extends along the length direction of the calibration body, and the center line of the liquid injection channel coincides with the center line of the calibration body.

[0009] In an embodiment of the utility model, the PCR calibration device further comprises a sealing member, which is used to completely seal the injection channel and isolate the fluorescent cavity from the outside.

[0010] In an embodiment of the utility model, the cavity wall of the fluorescent cavity forms a transmission surface for the excitation light source and the excitation fluorescence to transmit, and the transmission surface is set to be a spherical surface.

[0011] In an embodiment of the present invention, the cross-sectional size of the light guide portion is gradually reduced in a direction away from the main body portion, the light guide portion is formed with two light-transmitting surfaces, and the fluorescent cavity is disposed between the two light-transmitting surfaces.

[0012] In the embodiment of the utility model, a temperature measuring tank which is independent of the fluorescent cavity is provided on the calibration body, and a temperature measuring component is arranged in the temperature measuring tank.

[0013] In the embodiment of the utility model, a delivery channel which is independent of the fluorescent cavity is also provided on the calibration body, and the temperature measuring tank is connected with the outside world through the delivery channel.

[0014] In an embodiment of the utility model, the temperature measuring tank is filled with a heat-conducting layer, and the temperature measuring element is wrapped in the heat-conducting layer.

[0015] In the embodiment of the utility model, the fluorescent cavity is arranged corresponding to the excitation light source and the fluorescent receiving module, and the temperature measuring element is arranged in the fluorescent cavity;

[0016] and / or,

[0017] The phosphor is a phosphor sheet.

[0018] In an embodiment of the present invention, a PCR instrument is provided, comprising an excitation light source and the PCR calibration device as described above.

[0019] Through the above technical solution, the PCR calibration device and PCR instrument provided by the embodiment of the utility model have the following beneficial effects:

[0020] The PCR calibration device in the utility model is composed of a calibration body and a temperature measuring piece, and is used to calibrate the PCR instrument. The calibration body is used to be plugged into the detection cavity of the PCR instrument. The calibration body is provided with a main body and a light guide portion in sequence along the length direction. The light guide portion is provided with a fluorescent cavity filled with a fluorescent body, and an excitation light source is transmitted to the light guide portion to perform fluorescence excitation on the fluorescent body in the fluorescent cavity. The temperature measuring piece is installed on the calibration body and is used to calibrate the temperature of the PCR instrument. The PCR calibration device of the utility model has a fluorescent cavity filled with a fluorescent body on the light guide portion, and the fluorescent body is transmitted by an excitation light source and fluorescence excitation is performed on the fluorescent body. The temperature of the PCR instrument is calibrated by the temperature measuring piece, and temperature calibration and fluorescence calibration can be performed simultaneously, and the reliability of temperature calibration and fluorescence calibration is improved.

[0021] Other features and advantages of the embodiments of the present invention will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The accompanying drawings are used to provide a further understanding of the embodiments of the present utility model and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the embodiments of the present utility model, but do not constitute a limitation on the embodiments of the present utility model. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without creative work. In the drawings:

[0023] Figure 1 It is a structural schematic diagram of an embodiment of a PCR calibration device according to the utility model;

[0024] Figure 2 It is a structural schematic diagram of another embodiment of the PCR calibration device according to the utility model.

[0025] Description of Reference Numerals

[0026] 1 Calibration body 141 light-transmitting surface

[0027] 11 Fluorescence chamber 15 Temperature measuring chamber

[0028] 12 injection channel 16 delivery channel

[0029] 13 Main body 2 Temperature measuring element

[0030] 14 Light guide DETAILED DESCRIPTION

[0031] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the present invention.

[0032] The PCR calibration device and PCR instrument according to the present invention are described below with reference to the accompanying drawings.

[0033] In an embodiment of the utility model, a PCR calibration device is proposed, which is used to calibrate a PCR instrument with an excitation light source and a fluorescence receiving module. The PCR calibration device includes a calibration body 1 and a temperature measuring piece 2. The calibration body 1 is used to be plugged into the detection cavity of the PCR instrument. The calibration body 1 includes a main body 13 and a light guide 14 arranged in sequence along the length direction of the calibration body 1. The light guide 14 is for the excitation light source to transmit. The light guide 14 is provided with a fluorescence cavity 11, and the fluorescence cavity 11 is filled with a fluorescent body. The temperature measuring piece 2 is installed on the calibration body 1 and is used to calibrate the temperature of the PCR instrument. The existing PCR detection module is set on the PCR instrument. The PCR detection module includes an excitation light source, a fluorescence receiving module and a detection cavity. The PCR calibration device is mainly used for temperature and fluorescence calibration of the PCR detection module. Among them, the temperature measuring piece adopts a temperature sensor as in the prior art.

[0034] like Figure 1 As shown, in the embodiment of the utility model, the calibration body 1 and the temperature measuring part 2 are composed and used to calibrate the PCR instrument. The calibration body 1 is used to be plugged into the detection cavity of the PCR instrument. The calibration body 1 is sequentially provided with a main body 13 and a light guide part 14 along the length direction. The light guide part 14 is provided with a fluorescent cavity 11 for filling the fluorescent body. The excitation light source is transmitted to the light guide part 14 to perform fluorescence excitation on the fluorescent body in the fluorescent cavity 11. The emitted laser after fluorescence excitation will be transmitted and fed back to the PCR instrument. After receiving the calibrated fluorescence excitation value, the PCR instrument will compare and calibrate it with the preset fluorescence excitation value. The temperature measuring part 2 is installed on the calibration body 1 and is used to calibrate the temperature of the PCR instrument. When the temperature measuring part 2 measures the temperature of the fluorescent body, it is transmitted to the PCR instrument, and the measured calibration temperature is compared and calibrated with the preset temperature. The PCR calibration device in the utility model can perform temperature calibration and fluorescence calibration at the same time, improves the calibration time of temperature calibration and fluorescence calibration, and simplifies the operation process of temperature calibration and fluorescence calibration.

[0035] like Figure 1 and Figure 2 As shown, in an embodiment of the utility model, the calibration body 1 is provided with an injection channel 12 connected to the fluorescent cavity 11, and the injection channel 12 extends along the length direction of the calibration body 1, and the center line of the injection channel 12 coincides with the center line of the calibration body 1. The injection channel 12 extends along the length direction of the calibration body 1 to ensure that the reserve of the fluorescent body in the injection channel 12 is sufficient. The inner wall length of the injection channel 12 remains consistent, and the center line of the injection channel 12 coincides with the center line of the calibration body 1. This structural setting ensures that the center of gravity of the calibration body 1 will not shift when the fluorescent cavity 11 and the injection channel 12 are provided on the calibration body 1, thereby ensuring the stability of the calibration body 1 and ensuring that the calibration body 1 has good structural strength.

[0036] like Figure 1 and Figure 2 As shown, in an embodiment of the present utility model, the PCR calibration device further includes a sealing member, which is used to completely seal the injection channel 12 and isolate the sealed fluorescent cavity 11 from the outside.

[0037] like Figure 1 As shown, in one embodiment of the present invention, the excitation light source is arranged corresponding to the fluorescent cavity 11, the temperature measuring element 2 is arranged in the fluorescent cavity 11, and the fluorescent body is a fluorescent sheet. Among them, the fluorescent body in the present invention is divided into liquid fluorescent material and solid fluorescent material, and the solid fluorescent material adopts a fluorescent sheet.

[0038] Among them, in one embodiment of the utility model, firstly, a liquid solution of phosphor is injected into the injection channel 12, and a uniform mixed liquid made of UV glue or heat curing glue with good thermal conductivity and phosphor powder is selected. This phosphor has good thermal conductivity after curing. The phosphor enters the phosphor cavity 11 through the injection channel 12, and then the temperature measuring component 2 is loaded through the injection channel 12, so that the temperature measuring component 2 is immersed in the phosphor. When the phosphor is loaded, the phosphor liquid in the injection channel 12 and the phosphor cavity 11 is cured by UV light irradiation or heating, and the calibration experiment is convenient after curing.

[0039] It should be noted that in another embodiment of the present invention, the phosphor in the injection channel 12 and the fluorescent cavity 11 is maintained in liquid state, and it is only necessary to set a seal at the open end of the injection channel 12. After the phosphor and the sensor are loaded, the open end of the injection channel 12 is sealed, which can also ensure that the PCR calibration device can achieve similar temperature-fluorescence dual calibration. Under the condition that the phosphor is maintained in liquid state, the phosphor has a wider range of materials, but still needs to have good thermal conductivity, such as a general aqueous solution can fully meet the requirements.

[0040] In another embodiment of the present invention, the fluorescent sheet wrapped with the temperature measuring element 2 is directly placed in the fluorescent cavity 11. At this time, the temperature measuring element 2 can still perform temperature calibration normally.

[0041] like Figure 1 and Figure 2 As shown, in an embodiment of the present invention, the cavity wall of the fluorescent cavity 11 forms a transmission surface for the excitation light source to transmit. Preferably, the transmission surface is set to a spherical surface, which has a large contact area and can accommodate more fluorescent bodies. In another embodiment of the present invention, the transmission surface of the fluorescent cavity 11 is set to a diamond shape, which can still achieve a good fluorescence excitation effect and fully perform fluorescence excitation.

[0042] like Figure 1 and Figure 2 As shown, in an embodiment of the present utility model, the cross-sectional dimensions of the light guide portion 14 are gradually reduced in a direction away from the main body portion 13, and the light guide portion 14 is formed with two light-transmitting surfaces 141. The fluorescent cavity 11 is arranged between the two light-transmitting surfaces 141, so that the excitation light source and the excitation fluorescence can be better transmitted through the light-transmitting surfaces 141, thereby reducing the loss of the excitation and excitation fluorescence light source, which is beneficial for the excitation light source to fully excite and receive fluorescence on the fluorescent body.

[0043] like Figure 2As shown, in another embodiment of the utility model, a temperature measuring tank 15 independent of the fluorescent cavity 11 is provided on the calibration body 1, and a temperature measuring member 2 is arranged in the temperature measuring tank 15. In another embodiment of the utility model, a temperature measuring tank 15 independent of the fluorescent cavity 11 is provided on the calibration body 1, and the calibration positions of the temperature calibration and the fluorescence calibration are physically isolated, which can be applied to some application scenarios where the temperature probe and the fluorescent body are incompatible, and when the temperature probe is damaged, only the temperature probe needs to be replaced, without replacing the entire calibration device, which is convenient for repair and saves costs.

[0044] like Figure 2 As shown, in the embodiment of the utility model, the calibration body 1 is also provided with a delivery channel 16 which is independent of the fluorescent cavity 11, and the temperature measuring tank 15 is connected to the outside through the delivery channel 16. The temperature measuring tank 15 is filled with a heat conductive layer, and the temperature measuring member 2 is wrapped in the heat conductive layer. A gap filling material with good thermal conductivity, such as thermal conductive silicone grease, is added to the delivery channel 16, so that the temperature measuring tank 15 is filled with thermal conductive silicone grease, and the temperature measurement result of the temperature measuring member 2 is more accurate when wrapped in the thermal conductive silicone grease.

[0045] In another embodiment of the present invention, the main body 13 is first coated, and then the temperature measuring piece 2 is placed in the temperature measuring tank 15 through the conveying channel 16. At this time, the temperature measuring tank 15 is continuously filled with a filling material so that the filling material fully wraps the temperature measuring piece 2. The setting of the conveying channel 16 facilitates the assembly of the filling material.

[0046] In another embodiment of the present invention, the fluorescent sheet wrapped with the temperature measuring element 2 is directly placed in the fluorescent cavity 11, and the fluorescent sheet is structurally fixed, such as by coating, etc. At this time, the temperature measuring element 2 can still perform temperature calibration normally.

[0047] In an embodiment of the utility model, a PCR instrument is provided, comprising an excitation light source, a fluorescence receiving module and the PCR calibration device described above. Since the PCR instrument adopts all embodiments of the PCR calibration device, it also has all the beneficial effects brought by the PCR calibration device, which will not be described in detail here.

[0048] The angle formed by the incident direction of the excitation light source into the fluorescent cavity 11 and the emission direction of the excitation fluorescence from the fluorescent cavity 11 is R, R≤90°. Under this condition, the effect of the fluorescence calibration of the excitation light source is better and the result of the fluorescence calibration is more accurate.

[0049] In the description of the present invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0050] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0051] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0052] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Ordinary technicians in the field can change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A PCR calibration device for calibrating a PCR instrument with an excitation light source and a fluorescence receiving module, characterized in that: The PCR calibration device comprises: A calibration body (1) is used for plugging into the detection cavity of the PCR instrument. The calibration body (1) comprises a main body (13) and a light guide (14) arranged in sequence along the length direction of the calibration body. The light guide (14) is used for the excitation light source and the excitation fluorescence to transmit. A fluorescence cavity (11) is provided on the light guide (14), and the fluorescence cavity (11) is filled with a fluorescent substance. A temperature measuring component (2) is installed on the calibration body (1) and is used to calibrate the temperature of the PCR instrument.

2. The PCR calibration device according to claim 1, characterized in that: The calibration body (1) is provided with a liquid injection channel (12) connected to the fluorescent cavity (11); the liquid injection channel (12) extends along the length direction of the calibration body (1), and the center line of the liquid injection channel (12) coincides with the center line of the calibration body (1).

3. The PCR calibration device according to claim 2, characterized in that: The PCR calibration device also includes a sealing member, which is used to completely seal the injection channel (12) and isolate the fluorescent cavity (11) from the outside.

4. The PCR calibration device according to claim 1, characterized in that: The cavity wall of the fluorescent cavity (11) forms a transmission surface for the excitation light source to transmit, and the transmission surface is set as a spherical surface.

5. The PCR calibration device according to claim 1, characterized in that: The cross-sectional dimensions of the light-guiding portion (14) are gradually reduced in a direction away from the main body portion (13) from the light-guiding portion (14). The light-guiding portion (14) is formed with two light-transmitting surfaces (141), and the fluorescent cavity (11) is arranged between the two light-transmitting surfaces (141).

6. The PCR calibration device according to any one of claims 1 to 5, characterized in that: The calibration body (1) is provided with a temperature measuring chamber (15) which is independent of the fluorescent cavity (11), and the temperature measuring element (2) is arranged in the temperature measuring chamber (15).

7. The PCR calibration device according to claim 6, characterized in that: The calibration body (1) is also provided with a delivery channel (16) which is independent of the fluorescent cavity (11), and the temperature measuring tank (15) is connected to the outside world through the delivery channel (16).

8. The PCR calibration device according to claim 6, characterized in that: The temperature measuring tank (15) is filled with a heat-conducting layer, and the temperature measuring element (2) is wrapped in the heat-conducting layer.

9. The PCR calibration device according to any one of claims 1 to 5, characterized in that: The fluorescent cavity (11) is arranged corresponding to the excitation light source and the fluorescent receiving module, and the temperature measuring element (2) is arranged in the fluorescent cavity (11); and / or, The phosphor is a phosphor sheet.

10. A PCR instrument, characterized in that: The method comprises an excitation light source and the PCR calibration device according to any one of claims 1 to 9.

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