Small nucleic acid extraction system

Abstract

The utility model provides a small nucleic acid extraction system. The small nucleic acid extraction system comprises a vertical movement system and a horizontal movement system, the vertical movement system at least comprises a magnetic sleeve movement unit and a magnetic bar movement unit, the magnetic bar movement unit is fixed on the magnetic sleeve movement unit, and the magnetic sleeve movement unit and the magnetic bar movement unit can move vertically at the same time or at different times; the horizontal movement system at least comprises a horizontal movement control motor, a horizontal movement guide shaft, a tray heating module and a temperature control assembly on the tray heating module; the tray heating module can move back and forth under the control of a horizontal movement control motor; the nucleic acid extraction system further comprises a negative pressure suction filtration system located on the rear portion of the instrument, a horizontal movement unit and a magnetic sleeve movement unit and a magnetic bar movement unit which move vertically are controlled in a combined mode, and nucleic acid extraction operation can be carried out. And the device can be conveniently combined with a manipulator and PCR amplification equipment to form a full-automatic molecular analysis system.

Description

Technical Field

[0001] The utility model belongs to the technical field of nucleic acid extraction equipment, and particularly relates to a small nucleic acid extraction system. Background Art

[0002] Nucleic acid extractors are devices for extracting nucleic acids from samples. Nucleic acid extraction is widely used in disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial testing, food safety testing, animal husbandry and molecular biology research. Most of the previous nucleic acid extractors were large in size and required manual insertion of the magnetic sleeve on the magnetic sleeve rack, opening the door and inserting the nucleic acid extraction kit. Although the throughput was large, too many manual operation steps resulted in a cumbersome process and was not easy to automate and integrate.

[0003] At present, most molecular diagnostic analyses, from nucleic acid extraction to subsequent PCR amplification detection, have dedicated automated instruments. For the sake of convenience and anti-pollution, more and more applications and demands are concentrated on nucleic acid extraction and PCR amplification machines. The structure of existing nucleic acid extraction instruments makes it difficult to complete the operation of transferring consumables and nucleic acid extraction kits to the machine through mechanical devices, or even if it can be completed, it requires a large manipulator for transportation, which is time-consuming and labor-intensive, and the instrument is large in size. Therefore, we hope to design a nucleic acid extraction system with a new structure to solve this problem. Utility Model Content

[0004] In view of the shortcomings of the prior art, the purpose of the utility model is to provide a small nucleic acid extraction system to solve the problems raised in the above background technology.

[0005] A small nucleic acid extraction system comprises a vertical motion system and a horizontal motion system.

[0006] The vertical motion system at least comprises a magnetic sleeve motion unit and a magnetic rod motion unit. The magnetic rod motion unit is fixed on the magnetic sleeve motion unit. The two can move vertically simultaneously or in different time periods.

[0007] The magnetic sleeve motion unit at least comprises a magnetic sleeve motion control motor, a magnetic sleeve motion guide shaft, a magnetic sleeve connecting frame and a magnetic sleeve connecting piece. The one or more magnetic sleeve connecting pieces are fixed on the magnetic sleeve connecting frame.

[0008] The magnetic bar motion unit at least comprises a magnetic bar motion control motor, a magnetic bar motion guide shaft, a magnetic bar connecting frame and a magnetic bar. The one or more magnetic bars are fixed on the magnetic bar connecting frame. The vertical bottom end of the magnetic bar is a permanent magnet.

[0009] The magnetic sleeve connector is a hollow structure, and the one or more magnetic rods can be inserted into the hollow of the magnetic sleeve connector. The outside of the magnetic sleeve connector can be connected to the magnetic sleeve.

[0010] The horizontal motion unit at least includes a horizontal motion control motor, a horizontal motion guide shaft, a tray heating module and a temperature control component on the tray heating module.

[0011] The tray heating module temperature control component is located at the bottom of the tray heating module, and can provide the temperature environment required for nucleic acid extraction at a fixed position on the tray heating module. The tray heating module can carry a standard well plate. A magnetic sleeve can be placed in the standard well plate. The tray heating module can move forward and backward in and out of the bin under the control of the horizontal motion control motor, and be in different relative positions with the vertical motion system to perform nucleic acid extraction operations.

[0012] When taking the magnetic sleeve, the magnetic sleeve connector can move vertically downward under the action of the magnetic sleeve motion control motor, and the outer diameter of the magnetic sleeve connector is closely matched with the inner diameter of the magnetic sleeve in the standard orifice plate to obtain the magnetic sleeve.

[0013] When demagnetizing the sleeve, the magnetic rod passes through the magnetic sleeve connector and moves vertically downward under the action of the magnetic rod motion control motor until the bottom of the magnetic rod contacts the magnetic sleeve, and then continuously applies pressure to the bottom of the magnetic sleeve to overcome the friction between the outer diameter of the magnetic sleeve connector and the inner diameter of the magnetic sleeve until the magnetic sleeve is pushed out.

[0014] The nucleic acid extraction system includes a negative pressure filtration system located at the rear of the instrument, which can provide a negative pressure environment for the system to avoid contamination during the nucleic acid extraction process.

[0015] The nucleic acid extraction system also includes a liquid crystal control screen, through which the process can be set and controlled.

[0016] Due to the adoption of the above invention, the small nucleic acid extraction system has the following beneficial effects:

[0017] 1. The combined structure of magnetic rod and magnetic sleeve can effectively prevent pollution;

[0018] 2. The magnetic sleeve movement mechanism moves vertically to obtain the magnetic sleeve. When the magnetic sleeve is installed on the machine, it can be placed in a standard orifice plate to facilitate automated operation;

[0019] 3. The standard well plate is placed directly on the tray heating module, which is convenient for automatic transfer of the well plate in combination with the robot after leaving the warehouse, and can be quickly combined with the PCR amplification system. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0021] Figure 1 The utility model is a schematic diagram of the overall structure of a small nucleic acid extraction system.

[0022] Figure 2 This is a schematic diagram of the internal structure of a small nucleic acid extraction system of the utility model.

[0023] Figure 3 This is a schematic diagram of the tray heating module structure of a small nucleic acid extraction system of the utility model.

[0024] Figure 4 This is a schematic diagram of the lifting unit structure of a small nucleic acid extraction system of the utility model.

[0025] Figure 5 This is a schematic diagram of the magnetic rod structure of a small nucleic acid extraction system of the utility model.

[0026] Figure 6 This is a schematic diagram of the connection between a magnetic rod and a magnetic sleeve of a small nucleic acid extraction system of the utility model.

[0027] Figure 7 This is a schematic diagram of a magnetic sleeve structure of a small nucleic acid extraction system of the utility model.

[0028] In the figure,

[0029] 1-housing, 2-door, 3-LCD display, 4-deep well plate, 5-multi-connected magnetic sleeve, 6-tray heating module, 7-lifting unit, 8-platform unit, 9-filtration module, 10-control module, 11-disinfection module, 601-wiring, 602-base, 603-heating block, 604-heating element, 605-detection switch, 606-in and out rollers, 701-screw motor 1, 702-screw 1, 703-support, 704-magnetic rod, 705-nozzle, 706-circuit board, 707-screw 2, 708-screw motor 2. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0031] The utility model is realized by the following technical scheme: a small nucleic acid extraction system, comprising: a shell, a hatch, a liquid crystal display screen, a deep well plate, a multi-connected magnetic sleeve, a tray heating module, a lifting unit, a platform unit, a filtering module and a control module, wherein the left side of the platform unit is hinged to the lower side of the hatch through a torsion spring, and a liquid crystal display screen is installed on the upper left side of the shell;

[0032] A platform unit for driving the tray heating module to move left and right is installed at the bottom of the shell, the tray heating module is installed on the upper side of the platform unit, and a deep well plate is movably placed on the upper side of the tray heating module;

[0033] A multi-connected magnetic sleeve is placed on the upper side of the deep-hole plate, and a lifting unit is installed in the middle of the platform unit;

[0034] A control module is installed on the upper right side of the platform unit, and a disinfection module is installed below the left side of the control module and the right side of the lifting unit;

[0035] A filter module is installed on the inner wall of the platform unit and is placed on the right side of the tray heating module. The filter module includes a fan and a HEPA net, which are used to adsorb aerosols inside the instrument to avoid polluting the laboratory environment. The disinfection module is an ultraviolet disinfection module that can be controlled regularly to sterilize and disinfect the instrument. The control module includes a switching power supply, a main control board, a power switch, and a communication serial port, which provide stable voltage and communication control for the entire machine.

[0036] As a preferred embodiment, the deep-well plate is a 96-well deep-well plate, a multi-joint magnetic sleeve can be placed in each hole of the deep-well plate, and a buckle is provided on the inner wall of the upper end of each multi-joint magnetic sleeve.

[0037] As a preferred embodiment, the tray heating module includes a cable, a base, a heating block, a heating element, a detection switch and an in-and-out bin roller. A cable is arranged on the right side of the base, and a heating block is installed inside the left side of the base. In actual use, the platform unit includes a motor, a slide, and a belt. The tray heating module is slidably installed on the upper side of the slide, a driven pulley is installed on the lower side of the left end of the slide, the motor is installed on the right side of the slide, and a driving pulley is installed at the front end of the motor. The driven pulley is connected to the driving pulley through a belt drive, and the right side of the upper surface of the belt is fixedly connected to the right side of the lower surface of the tray heating module. The setting of the platform unit allows the tray heating module to move left and right.

[0038] As a preferred embodiment, a heating element is installed inside the left side of the base, and the heating element is abutted against the heating block. A detection switch is installed inside the left end of the base. A loading and unloading roller is rotatably installed on the front and rear sides of the left end of the base. In actual use, the leftmost end of the loading and unloading roller is located on the left side of the leftmost end of the deep-hole plate. When exiting the warehouse, the deep-hole plate will not contact the inner wall of the warehouse door first.

[0039] As a preferred embodiment, the lifting platform includes a screw rod 1, a screw rod motor 1, a support, and a magnetic rod. The lower end of the screw rod is rotatably connected to the bottom of the shell through the support, the support is fixedly connected to the bottom of the shell, and the top of the screw rod 1 is equipped with a screw rod motor 1;

[0040] A screw nut 1 is movably mounted on the upper side of the screw rod 1.

[0041] As a preferred embodiment, a mounting plate is provided on the left side of the nut 1, and a screw rod 2 is rotatably mounted on the upper side of the mounting plate. The upper end of the screw rod 2 is fixedly connected to the screw motor 2, and the front side of the screw motor 2 is fixedly connected to the front inner wall of the housing.

[0042] A screw rod 2 is rotatably installed on the lower side of the screw motor 2, a nut 2 is movably installed on the upper side of the screw rod 2, a strip plate is fixed on the upper side of the nut 2, a magnetic rod is respectively installed on the left and right sides of the strip plate, and a nozzle is provided on the upper side of each of the magnetic rods. In actual use, the nut 1 and the nut 2 are both provided with corresponding guide rod structures, that is, the nut 1 and the nut 2 will not deflect during movement and will only move in the up and down directions, thereby ensuring the operating stability of the entire lifting unit.

[0043] After adopting the above technical solution, the beneficial effects of the utility model are: the setting of the movable door and the platform unit, the standard deep-well plate is directly placed on the tray heating module, which is convenient for combining with the robot to automatically transfer the well plate after leaving the warehouse, and can quickly realize the combination with the PCR amplification system;

[0044] The lifting unit is set up to realize the actions of adsorbing magnetic beads, transferring magnetic beads and unloading the multi-joint magnetic sleeve. The combined structure of magnetic rod and magnetic sleeve can effectively prevent contamination. The lifting unit obtains the multi-joint magnetic sleeve through vertical movement. The multi-joint magnetic sleeve can be placed in a standard orifice plate when it is installed on the machine, which is convenient for automated operation.

[0045] Embodiment 1:

[0046] See also Figures 1 to 7 The utility model provides a technical solution: a small nucleic acid extraction system, including: a shell 1, a hatch, a liquid crystal display screen 3, a deep well plate 4, a multi-connected magnetic sleeve 5, a tray heating module 6, a lifting unit 7, a platform unit 8, a filtering module 9 and a control module 10, the left side of the shell 1 is hinged with a hatch through a torsion spring, and the upper left side of the shell 1 is equipped with a liquid crystal display screen 3;

[0047] A platform unit 8 for driving the tray heating module 6 to move left and right is installed at the bottom of the housing 1, the tray heating module 6 is installed on the upper side of the platform unit 8, and a deep well plate 4 is movably placed on the upper side of the tray heating module 6;

[0048] A multi-connected magnetic sleeve 5 is placed on the upper side of the deep hole plate 4, and a lifting unit 7 is installed in the middle of the platform unit 8;

[0049] A control module 10 is installed on the upper right side of the platform unit 8, and a disinfection module 11 is installed below the left side of the control module 10 and the right side of the lifting unit 7;

[0050] A filter module 9 is installed on the right inner wall of the platform unit 8. The filter module 9 is placed on the right side of the tray heating module 6. The filter module 9 includes a fan and a HEPA net, which is used to adsorb aerosols inside the instrument to avoid polluting the laboratory environment. The disinfection module 11 is an ultraviolet disinfection module 11, which can be controlled regularly to sterilize and disinfect the instrument. The control module 10 includes a switching power supply, a main control board, a power switch, and a communication serial port to provide stable voltage and communication control for the entire machine.

[0051] See also Figures 1 to 3 The deep-well plate 4 is a 96-well deep-well plate 4 , and a multi-joint magnetic sleeve 5 can be placed in each hole of the deep-well plate 4 , and a buckle is provided on the inner wall of the upper end of each multi-joint magnetic sleeve 5 .

[0052] The tray heating module 6 includes a cable 601, a base 602, a heating block 603, a heating element 604, a detection switch 605 and an in-and-out roller 606. The cable 601 is arranged on the right side of the base 602, and the heating block 603 is installed inside the left side of the base 602. In actual use, the platform unit 8 includes a motor, a slide, and a belt. The tray heating module 6 is slidably installed on the upper side of the slide, and a driven pulley is installed on the lower side of the left end of the slide. The motor is installed on the right side of the slide, and a driving pulley is installed on the front end of the motor. The driven pulley is connected to the driving pulley through a belt drive. The right side of the upper surface of the belt is fixedly connected to the right side of the lower surface of the tray heating module 6. The setting of the platform unit 8 allows the tray heating module 6 to move left and right.

[0053] A heating element 604 is installed inside the left side of the base 602, and the heating element 604 is abutted against the heating block 603. A detection switch 605 is installed inside the left end of the base 602. A storage tank entry and exit roller 606 is rotatably installed on the front and rear sides of the left end of the base 602. In actual use, the leftmost end of the storage tank entry and exit roller 606 is located on the left side of the leftmost end of the deep hole plate 4. When exiting the storage tank, the deep hole plate 4 will not contact the inner wall of the storage tank door 2 first.

[0054] Embodiment 2:

[0055] In actual use, the platform unit 8 is started to drive the tray heating module 6 thereon to move to the left (if the tray heating module 6 needs to move to the left, the motor on the platform unit 8 rotates counterclockwise, so that the belt drives the tray heating module 6 to move to the left, and when the in-and-out warehouse roller 606 on the tray heating module 6 contacts the lower inner wall of the warehouse door 2, under the push of the in-and-out warehouse roller 606, the lower side of the warehouse door 2 rotates counterclockwise around the torsion spring, and the warehouse door 2 gradually opens, thereby causing the entire tray heating module 6 to gradually extend out of the shell 1 (in actual use, the shell 1 is installed and buckled last, except for the part pre-installed with the shell 1, the rest are first installed together, installed on the platform unit 8, and finally buckled). When the in-and-out warehouse roller 606 contacts the warehouse door 2, it rolls, thereby avoiding motion interference. When the motor rotates clockwise to retract the tray heating module 6, the warehouse door 2 is acted on by the torsion spring. 4 and then rotate clockwise until it is completely closed). After the tray heating module 6 extends out of the housing 1, the multi-connected magnetic sleeve 5 is loaded into the hole of the 96 deep-well plate 4. Subsequently, the 96 deep-well plate 4 is loaded onto the tray heating module 6. Then, the platform unit 8 is controlled to move the tray heating module 6 together with the deep-well plate 4 and the multiple multi-connected magnetic sleeves 5 placed thereon to the right. After the deep-well plate 4 is moved to a suitable position, the platform unit 8 is stopped. Then, the lifting unit 7 can be started to perform nucleic acid extraction. The standard deep-well plate 4 is directly placed on the tray heating module 6, which is convenient for combining with the robot to automatically transfer the well plate after leaving the warehouse. The combination with the PCR amplification system can be quickly realized (the heating element 604 is a heating film. After generating heat, the heating block 603 is heated by contact, that is, the metal heating block 603 is kept at the same temperature as the heating element 604, thereby performing contact heating on the deep-well plate 4 placed on the heating block 603).

[0056] See also Figures 1 to 7 The lifting platform includes a screw rod 702, a screw motor 701, a support 703, and a magnetic rod 704. The lower end of the screw rod 702 is rotatably connected to the bottom of the housing 1 through the support 703, the support 703 is fixedly connected to the bottom of the housing 1, and a screw motor 701 is installed on the top of the screw rod 702;

[0057] A screw nut 1 is movably mounted on the upper side of the screw rod 1 702.

[0058] A mounting plate is provided on the left side of the nut 1, and a screw rod 2 707 is rotatably mounted on the upper side of the mounting plate. The upper end of the screw rod 2 707 is fixedly connected to the screw motor 2 708, and the front side of the screw motor 2 708 is fixedly connected to the front inner wall of the housing 1.

[0059] A screw rod 2 707 is rotatably installed on the lower side of the screw motor 2 708, and a nut 2 is movably installed on the upper side of the screw rod 2 707. A strip plate is fixed on the upper side of the nut 2, and a magnetic rod 704 is respectively installed on the left and right sides of the strip plate. A nozzle 705 is arranged on the upper side of each magnetic rod 704. In actual use, the nut 1 and the nut 2 are both provided with corresponding guide rod structures, that is, the nut 1 and the nut 2 will not deflect during movement, and will only move in the up and down directions, thereby ensuring the operating stability of the entire lifting unit 7.

[0060] Embodiment 3:

[0061] Based on Example 2, in actual use, after the deep hole plate 4 moves to a suitable position, the screw motor 1 701 is started first, the screw motor 1 701 drives the screw 1 702 to rotate, and drives the nut 1 on the screw 1 702 to move downward. After moving to the predetermined position, the screw motor 1 701 stops running, and then the screw motor 2 708 is started, and the screw motor 2 708 mobilizes the screw 2 707 to rotate, thereby causing the nut 2 to move downward, and the nut 2 drives the strip plate to move downward, and synchronously drives the magnetic rod 704 installed on the strip plate. Moving downward, the magnetic rod 704 moves down to the inside of the multi-joint magnetic sleeve 5 just below the corresponding position, and the nozzle 705 on the magnetic rod 704 is engaged with the buckle on the inner wall of the multi-joint magnetic sleeve 5, so that the magnetic rod 704 can be loaded with the multi-joint magnetic sleeve 5 without falling off, thereby realizing the actions of adsorbing magnetic beads, transferring magnetic beads and unloading the multi-joint magnetic sleeve 5. The combined structure of the magnetic rod 704 and the magnetic sleeve can effectively prevent contamination. The lifting unit 7 obtains the multi-joint magnetic sleeve 5 through vertical movement, and the multi-joint magnetic sleeve 5 can be placed in a standard orifice plate when it is on the machine, which is convenient for automated operation.

[0062] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A small nucleic acid extraction system, characterized in that: Including vertical motion system and horizontal motion system; The vertical motion system at least includes a magnetic sleeve motion unit and a magnetic rod motion unit, the magnetic rod motion unit is fixed on the magnetic sleeve motion unit, and the two can move vertically simultaneously or in different time periods; The horizontal motion system at least includes a horizontal motion control motor, a horizontal motion guide shaft, a tray heating module, and a temperature control component on the tray heating module; the tray heating module can move forward and backward under the control of the horizontal motion control motor; The nucleic acid extraction system also includes a negative pressure filtration system located at the rear of the instrument.

2. The small-scale nucleic acid extraction system according to claim 1, characterized in that: The magnetic sleeve motion unit at least comprises a magnetic sleeve motion control motor, a magnetic sleeve motion guide shaft, a magnetic sleeve connecting frame and a magnetic sleeve connecting piece, and the magnetic sleeve connecting piece is fixed on the magnetic sleeve connecting frame.

3. The small-scale nucleic acid extraction system according to claim 1, characterized in that: The magnetic rod motion unit at least comprises a magnetic rod motion control motor, a magnetic rod motion guide shaft, a magnetic rod connecting frame and a magnetic rod, and the magnetic rod is fixed on the magnetic rod connecting frame.

4. The small-scale nucleic acid extraction system according to claim 1, characterized in that: The magnetic sleeve connecting piece is a hollow structure, and the magnetic rod can be inserted into the hollow of the magnetic sleeve connecting piece; the outside of the magnetic sleeve connecting piece is connected to the magnetic sleeve.

5. The small-scale nucleic acid extraction system according to claim 1, characterized in that: The temperature control component on the tray heating module is located at the bottom of the tray heating module, and provides a temperature environment required for nucleic acid extraction at a fixed position on the tray heating module.

6. The small-scale nucleic acid extraction system according to claim 1, characterized in that: The tray heating module carries a standard orifice plate, and a magnetic sleeve is placed in the standard orifice plate.

7. The small-scale nucleic acid extraction system according to claim 2, characterized in that: The outer diameter of the magnetic sleeve connector closely matches the inner diameter of the magnetic sleeve in the standard orifice plate.

8. The small-scale nucleic acid extraction system according to claim 2, characterized in that: The magnetic sleeve connecting piece can move vertically downward under the action of the magnetic sleeve motion control motor.

9. The small-scale nucleic acid extraction system according to claim 3, characterized in that: The magnetic bar can pass through the magnetic sleeve connecting piece and move vertically downward under the action of the magnetic bar movement control motor until the bottom of the magnetic bar contacts the magnetic sleeve.

10. The small-scale nucleic acid extraction system according to claim 1, characterized in that: The nucleic acid extraction system also includes a liquid crystal control screen.

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