Tumor organoid culture medium
By designing operating structures in tumor organoid culture medium, including motors, bidirectional threaded rods and infusion nozzles, the problem of traditional equipment requiring manual operation to add nutrient solution is solved, and automated production is achieved, reducing costs and improving production efficiency.
Patent Information
- Application Number
- CN202420928502.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-30
- Publication Date
- 2024-12-24
- Estimated Expiration
- 2034-04-30
AI Technical Summary
Traditional tumor organoid culture media is difficult to automatically add nutrient solution during the production process, and requires manual operation, resulting in high labor costs and inconvenient mass production.
A tumor organoid culture medium including operating support and connecting columns was designed. The function of automatic addition of nutrient solution is realized by setting up an operating structure of a motor, a bidirectional threaded rod, a transmission gear, an infusion nozzle and a distance sensor.
It realizes the convenience of automatic addition of nutrient solution, reduces labor costs, and improves the efficiency of mass production.
Smart Images
Figure CN222226337U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of culture medium, in particular to a tumor organoid culture medium. Background Art
[0002] With the development of the times and the continuous progress of the medical field, in order to meet the actual use needs of the modern medical field and produce cultures with similar structures and functions to organs in the body through in vitro three-dimensional culture, composed of a variety of cells including stem cells, with the potential to differentiate into tumor organoids, can better represent the physiological and genetic characteristics of the body, and then treat patients, etc., culture media are needed to culture tumor organoids, so as to meet the use needs and further promote the development of the medical field;
[0003] Traditional tumor organoid culture media are difficult to automatically add nutrient solution during the production process, requiring manual operation, which greatly increases labor costs and has certain limitations in the mass production process. Utility Model Content
[0004] The utility model aims to provide a tumor organoid culture medium, so as to solve the defect that the existing tumor organoid culture medium is difficult to conveniently carry out automated production.
[0005] In order to solve the above technical problems, the utility model provides the following technical solutions: a tumor organoid culture medium, comprising an operation support and a connecting column;
[0006] A connecting column is passed through the interior of the operation support, an operating table is arranged at the top of the connecting column, and a limiting structure is evenly arranged at the top of the operating table;
[0007] An operating frame is provided on one side of the operating support, and an operating structure is provided at the bottom end of the operating frame;
[0008] The operating structure includes a motor, a bidirectional threaded rod, a first bevel gear, a transmission shaft, a transmission gear, a second bevel gear, a reserved groove, an adjustment block, an electric push rod, an infusion seat, an infusion nozzle and a distance sensor. The motor is arranged at the bottom end of the operating frame, and a bidirectional threaded rod is arranged at the top of the motor. A first bevel gear is arranged on the outer wall of the bidirectional threaded rod below the operating frame, a transmission shaft is arranged on one side of the first bevel gear, transmission gears are arranged at both ends of the transmission shaft, and a second bevel gear is arranged on the top of the transmission gear. The reserved groove is opened on one side of the top of the operating frame, an adjustment block is arranged on the outer wall of the top of the bidirectional threaded rod, an electric push rod is arranged on one side of the adjustment block, and an infusion seat is arranged on the side of the electric push rod away from the adjustment block, infusion nozzles are evenly arranged at the bottom of the infusion seat, and distance sensors are arranged on both sides of the infusion nozzle at the bottom of the infusion seat.
[0009] Preferably, the limiting structure includes a limiting groove, a mounting plate, a reset spring, a clamping groove, a clamping plate and a culture medium body, the limiting grooves are evenly opened at the top of the operating table, the limiting grooves are provided with mounting plates inside, the bottom ends of the mounting plates are provided with reset springs, the operating table outside the limiting grooves is provided with clamping grooves, the outer walls of the mounting plates are provided with clamping plates, and the top of the mounting plates is provided with a culture medium body.
[0010] Preferably, the limiting grooves are distributed at equal intervals on the top of the operating table, and the side of the return spring away from the mounting plate is fixedly connected to the bottom end of the limiting groove.
[0011] Preferably, the clamping plates are all arranged inside the clamping grooves, and the top of one side of the clamping plates extends to the outside of the operating table.
[0012] Preferably, the mounting plate and the snap-in groove both form a snap-in structure through the snap-in plate, and the culture medium body is arranged inside the limiting groove.
[0013] Preferably, the output end of the motor is connected to the input end of the bidirectional threaded rod, and the first bevel gear is meshed with the transmission gear.
[0014] Preferably, the transmission gear is meshed with the No. 2 bevel gear, and the top end of the No. 2 bevel gear is fixedly connected to the bottom end of the connecting column.
[0015] Preferably, the top end of the bidirectional threaded rod extends to the inside of the reserved groove, and the electric push rod is threadedly connected to the adjustment block.
[0016] Preferably, the distance sensor is externally connected to a single-chip microcomputer, and the output end of the single-chip microcomputer is electrically connected to the input end of the infusion nozzle.
[0017] The utility model provides a tumor organoid culture medium, which has the advantages of:
[0018] By providing an operating structure, the starting motor drives the adjusting block to reciprocate inside the reserved groove through the rotation of the bidirectional threaded rod, and drives the connecting column to rotate through two sets of meshing gears, thereby causing the operating table on the top to rotate synchronously. The electric push rod can adjust the distance between the infusion seat and the limit groove, thereby facilitating the addition of nutrient solution, and distance sensors are provided on both sides of the infusion nozzle, so that it is convenient to start the infusion nozzle intermittently by sensing the distance between the infusion seat and the operating table, thereby facilitating the automatic addition of nutrient solution to the rotating culture medium body, thereby achieving the purpose of facilitating automatic addition of nutrient solution and convenient operation. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1It is a three-dimensional structural schematic diagram of the utility model;
[0020] Figure 2 It is a front view cross-sectional structural schematic diagram of the utility model;
[0021] Figure 3 It is a schematic diagram of the enlarged structure of point A of the utility model;
[0022] Figure 4 It is a schematic diagram of the top view structure of the utility model;
[0023] Figure 5 It is a schematic diagram of the partial cross-sectional structure of the utility model.
[0024] Explanation of the reference numerals in the figure: 1. operating support; 2. connecting column; 3. operating table; 4. limiting structure; 401. limiting groove; 402. mounting plate; 403. reset spring; 404. snap-in groove; 405. snap-in plate; 406. culture medium body; 5. operating frame; 6. operating structure; 601. motor; 602. bidirectional threaded rod; 603. bevel gear No. 1; 604. transmission shaft; 605. transmission gear; 606. bevel gear No. 2; 607. reserved groove; 608. adjustment block; 609. electric push rod; 610. infusion seat; 611. infusion nozzle; 612. distance sensor. DETAILED DESCRIPTION
[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0026] See also Figure 1-Figure 5 The utility model provides a tumor organoid culture medium, which includes an operation support 1 and a connecting column 2.
[0027] Reference Figure 4 and Figure 5As shown, a connecting column 2 runs through the interior of the operating support 1, an operating table 3 is arranged at the top of the connecting column 2, a limiting structure 4 is evenly provided at the top of the operating table 3, the limiting structure 4 includes a limiting groove 401, a mounting plate 402, a reset spring 403, a clamping groove 404, a clamping plate 405 and a culture medium body 406, the limiting groove 401 is evenly provided at the top of the operating table 3, the limiting groove 401 is provided with a mounting plate 402, the bottom end of the mounting plate 402 is provided with a reset spring 403, and a clamping groove 404 is provided inside the operating table 3 outside the limiting groove 401. A clamping plate 405 is provided on the outer wall of the mounting plate 402, a culture medium body 406 is provided at the top of the mounting plate 402, the limiting grooves 401 are evenly spaced at the top of the operating table 3, the side of the reset spring 403 away from the mounting plate 402 is fixedly connected to the bottom end of the limiting groove 401, the clamping plate 405 is arranged inside the clamping groove 404, the top of one side of the clamping plate 405 extends to the outside of the operating table 3, the mounting plate 402 and the clamping groove 404 form a clamping structure through the clamping plate 405, and the culture medium body 406 is arranged inside the limiting groove 401.
[0028] In order to meet the actual use needs in the modern medical field and produce cultures with similar structures and functions to organs in the body through in vitro three-dimensional culture, which are composed of a variety of cells including stem cells and have the potential to differentiate into tumor organoids and can better represent the physiological and genetic characteristics of the body, it is necessary to produce tumor-like tracheal culture media, but traditional production equipment is difficult to conveniently limit the main body of the culture medium, and thus has certain limitations in the production process. By setting a limited structure 4, it is easy to position and install, easy to take out and easy to operate, thereby greatly increasing the ease of use of the device.
[0029] Reference Figure 1-Figure 4As shown, an operating frame 5 is provided on one side of the operating support 1, and an operating structure 6 is provided at the bottom end of the operating frame 5. The operating structure 6 includes a motor 601, a bidirectional threaded rod 602, a first bevel gear 603, a transmission shaft 604, a transmission gear 605, a second bevel gear 606, a reserved groove 607, an adjustment block 608, an electric push rod 609, an infusion seat 610, an infusion nozzle 611 and a distance sensor 612. The motor 601 is provided at the bottom end of the operating frame 5, and a bidirectional threaded rod 602 is provided on the top of the motor 601. A first bevel gear 603 is provided below the operating frame 5 on the outer wall of the bidirectional threaded rod 602, a transmission shaft 604 is provided on one side of the first bevel gear 603, transmission gears 605 are provided at both ends of the transmission shaft 604, and a second bevel gear 606 is provided on the top of the transmission gear 605. The reserved groove 607 is opened on one side of the top of the operating frame 5, and the bidirectional threaded rod 602 is provided on the top of the bidirectional threaded rod 602. An adjusting block 608 is arranged on the outer wall of the part, an electric push rod 609 is arranged on one side of the adjusting block 608, an infusion seat 610 is arranged on the side of the electric push rod 609 away from the adjusting block 608, infusion nozzles 611 are evenly arranged at the bottom of the infusion seat 610, and distance sensors 612 are arranged on both sides of the infusion nozzles 611 at the bottom of the infusion seat 610. The output end of the motor 601 is connected to the input end of the bidirectional threaded rod 602, the No. 1 bevel gear 603 is meshed with the transmission gear 605, the transmission gear 605 is meshed with the No. 2 bevel gear 606, the top of the No. 2 bevel gear 606 is fixedly connected to the bottom end of the connecting column 2, the top of the bidirectional threaded rod 602 extends to the inside of the reserved groove 607, the electric push rod 609 is threadedly connected to the adjusting block 608, and the distance sensor 612 is externally connected to a single-chip microcomputer, and the output end of the single-chip microcomputer is electrically connected to the input end of the infusion nozzle 611.
[0030] In the process of producing the culture medium, it is necessary to add nutrients thereto, and in the process of producing it with traditional production equipment, manual operation is required, which makes it have certain limitations in the process of production and use, and greatly increases the labor cost and is not convenient for mass production. By providing an operating structure 6, the device is convenient for automatically adding nutrient solution, thereby reducing labor costs and facilitating operation and use.
[0031] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art may still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.
Claims
1. A tumor organoid culture medium, comprising an operation support (1) and a connecting column (2); Features: A connecting column (2) is passed through the interior of the operating support (1); an operating table (3) is arranged at the top of the connecting column (2); and a limiting structure (4) is evenly arranged at the top of the operating table (3); An operating frame (5) is provided on one side of the operating support (1), and an operating structure (6) is provided at the bottom end of the operating frame (5); The operating structure (6) comprises a motor (601), a bidirectional threaded rod (602), a first bevel gear (603), a transmission shaft (604), a transmission gear (605), a second bevel gear (606), a reserved groove (607), an adjustment block (608), an electric push rod (609), an infusion stand (610), an infusion nozzle (611) and a distance sensor (612); the motor (601) is arranged at the bottom end of the operating frame (5); a bidirectional threaded rod (602) is arranged at the top end of the motor (601); a first bevel gear (603) is arranged on the outer wall of the bidirectional threaded rod (602) below the operating frame (5); and a transmission shaft (604) is arranged on one side of the first bevel gear (603). , both ends of the transmission shaft (604) are provided with transmission gears (605), the top of the transmission gear (605) is provided with a second bevel gear (606), the reserved groove (607) is opened on one side of the top of the operating frame (5), an adjustment block (608) is provided on the outer wall of the top of the bidirectional threaded rod (602), one side of the adjustment block (608) is provided with an electric push rod (609), and the side of the electric push rod (609) away from the adjustment block (608) is provided with an infusion seat (610), the bottom end of the infusion seat (610) is evenly provided with infusion nozzles (611), and distance sensors (612) are provided on both sides of the infusion nozzle (611) at the bottom of the infusion seat (610).
2. A tumor organoid culture medium according to claim 1, characterized in that: The limiting structure (4) comprises a limiting groove (401), a mounting plate (402), a return spring (403), a snap-in groove (404), a snap-in plate (405) and a culture medium body (406); the limiting groove (401) is evenly arranged at the top of the operating table (3); a mounting plate (402) is arranged inside the limiting groove (401); a return spring (403) is arranged at the bottom end of the mounting plate (402); a snap-in groove (404) is arranged inside the operating table (3) outside the limiting groove (401); a snap-in plate (405) is arranged on the outer wall of the mounting plate (402); and a culture medium body (406) is arranged at the top of the mounting plate (402).
3. A tumor organoid culture medium according to claim 2, characterized in that: The limiting grooves (401) are distributed at equal intervals on the top of the operating table (3), and the side of the return spring (403) away from the mounting plate (402) is fixedly connected to the bottom end of the limiting groove (401).
4. A tumor organoid culture medium according to claim 2, characterized in that: The clamping plates (405) are all arranged inside the clamping grooves (404), and the top of one side of the clamping plates (405) extends to the outside of the operating table (3).
5. The tumor organoid culture medium according to claim 2, characterized in that: The mounting plate (402) and the clamping groove (404) both form a clamping structure through the clamping plate (405), and the culture medium body (406) is arranged inside the limiting groove (401).
6. The tumor organoid culture medium according to claim 1, characterized in that: The output end of the motor (601) is connected to the input end of the bidirectional threaded rod (602), and the first bevel gear (603) is meshed with the transmission gear (605).
7. The tumor organoid culture medium according to claim 1, characterized in that: The transmission gear (605) is meshed with the second bevel gear (606), and the top end of the second bevel gear (606) is fixedly connected to the bottom end of the connecting column (2).
8. The tumor organoid culture medium according to claim 1, characterized in that: The top end of the bidirectional threaded rod (602) extends to the inside of the reserved groove (607), and the electric push rod (609) and the adjustment block (608) are threadedly connected.
9. The tumor organoid culture medium according to claim 1, characterized in that: The distance sensor (612) is externally connected to a single-chip microcomputer, and the output end of the single-chip microcomputer is electrically connected to the input end of the infusion nozzle (611).