Temperature testing device for enzyme activity testing
By using a rotating plate and a shake flask in the enzyme activity test device, the problem of uneven heating of the existing device is solved, the accuracy of the test data is improved, and multiple enzyme activity tests are supported.
Patent Information
- Application Number
- CN202420006761.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-01-03
- Publication Date
- 2024-12-24
- Estimated Expiration
- 2034-01-03
AI Technical Summary
The existing enzyme activity testing device has a large bottom end and a small top end, which leads to uneven heating, resulting in inaccurate test data.
A temperature test device including a test chamber, a test chamber distributed in multiple rectangular arrays, a bottom heating plate, a rotating seat, a rotating gear, a driving motor, a rotating plate and an insulation plate are designed, and the rotating plate is rotated parallel to the shaker bottle. Ensure even heat distribution.
The uniform heating of enzyme activity test is achieved, which improves the accuracy of the test data and allows multiple enzyme activity tests to be performed simultaneously, enriching the test data.
Smart Images
Figure CN222226386U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of enzyme activity test, in particular to a temperature test device for enzyme activity test. Background Art
[0002] Enzymes are proteins or RNAs produced by living cells that are highly specific and catalytic to their substrates. The catalytic effect of enzymes depends on the integrity of the primary structure and spatial structure of the enzyme molecule. The catalytic effect of enzymes is closely related to the activity of the enzyme.
[0003] Enzyme activity refers to the ability of an enzyme to catalyze a certain chemical reaction. The size of the enzyme activity can be expressed by the conversion rate of a chemical reaction catalyzed by it under certain conditions. That is, the faster the enzyme-catalyzed conversion rate, the higher the enzyme activity; conversely, the slower the rate, the lower the enzyme activity. The activity of the enzyme is related to factors such as pH value and temperature, so the enzyme activity needs to be tested before use. During the test, equal amounts of enzymes need to be placed in different shake flasks and each shake flask needs to be heated at different temperatures.
[0004] Since the shake flask is shaped like a cone with a large bottom and a small top, the shake flask is heated unevenly during heating, making the test data inaccurate. Therefore, it does not meet the existing needs. We propose a temperature test device for enzyme activity testing. Utility Model Content
[0005] The purpose of the utility model is to provide a temperature test device for enzyme activity testing, so as to solve the problems raised in the above-mentioned background technology that the shaking bottle is formed with a large bottom and a small top and is cone-shaped as a whole, resulting in uneven heating of the shaking bottle during heating, making the data obtained from the test inaccurate, etc.
[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a temperature test device for enzyme activity testing, comprising a test box, a surface of the test box is provided with a plurality of test chambers distributed in a rectangular array, a bottom heating plate is fixed to the bottom surface of the test chamber, a rotating seat is rotatably installed on the outer side of the bottom heating plate, a rotating gear is fixed to the outer surface of the rotating seat, a driving gear is meshedly connected to one side of the rotating gear, a driving motor is installed below the driving gear, the output shaft end of the driving motor is fixed to the driving gear, a plurality of symmetrically distributed rotating plates are installed on the inner side of the rotating seat, a rotating plate hot plate is inlaid on the inner surface of the rotating plate, the bottom end of the rotating plate is rotatably connected to the rotating seat by an axle pin, a wire metal ring is inlaid on the outer surface of the bottom end of the rotating seat, a conductive block is fitted on the outer side of the wire metal ring, and a storage power supply is installed below the rotating gear.
[0007] Preferably, the storage power source is connected to the driving motor and the conductive block by wires, and the wire metal ring is connected to the rotating plate, the hot plate and the bottom heating plate by wires.
[0008] Preferably, a plurality of symmetrical electric telescopic rods are fixed on the inner wall of the rotating seat, and the number of the electric telescopic rods is consistent with the number of the rotating plates.
[0009] Preferably, a lifting plate is fixed to the movable end of the electric telescopic rod, the lifting plate is slidably connected to the rotating seat, two connecting plates are fixed to the outer surface of the rotating plate, and one end of the lifting plate is located between the two connecting plates.
[0010] Preferably, a connecting pin is fixed through the end of the lifting plate between the two connecting plates, and a connecting slot is provided through the connecting plates.
[0011] Preferably, both ends of the connecting slide groove penetrate the side wall of the lifting plate and are slidably engaged in the connecting slide groove, and the end of the connecting pin is flush with the outer wall of the connecting plate.
[0012] Preferably, a heat preservation plate is detachably mounted on the top of the test chamber, and two symmetrically distributed separation blocks are fixed to the upper surface of the heat preservation plate, and the top of the separation block is bent in a U shape toward the edge of the heat preservation plate.
[0013] Compared with the prior art, the beneficial effects of the utility model are:
[0014] 1. The utility model installs a plurality of symmetrical and rotatable rotating plates and rotating plate hot plates on the rotating seat, and makes the rotating plate hot plates parallel to the outer surface of the shaking bottle. When the rotating plate hot plates are powered on for heating, the rotating seat drives the rotating plate hot plates to rotate around the medicine bottles, so that the medicine bottles are heated evenly, and the test data is guaranteed to be more accurate.
[0015] 2. The utility model provides a plurality of test chambers distributed in an array in the test box, and uses a detachable insulation board to seal the top of the test chamber, and a circular hole is provided in the center of the insulation board, so that multiple shaking bottles can be used for enzyme activity tests at the same time to enrich the test data. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0017] Figure 2 A cutaway view of the utility model as a whole;
[0018] Figure 3 For this utility model Figure 2 Schematic diagram of the local structure at A in the middle;
[0019] Figure 4 For this utility model Figure 2 Schematic diagram of the local structure at point B in the figure.
[0020] In the figure: 1. test box; 2. insulation board; 3. separation pull block; 4. wire metal ring; 5. test chamber; 6. bottom heating plate; 7. rotating seat; 8. rotating gear; 9. rotating plate; 10. electric telescopic rod; 11. driving gear; 12. lifting plate; 13. storage power supply; 14. rotating plate hot plate; 15. connecting plate; 16. connecting slide; 17. connecting pin; 18. driving motor; 19. conductive block. DETAILED DESCRIPTION
[0021] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.
[0022] The driving motor (model 5IK60RGN-CF) and the electric telescopic rod (model MAL) mentioned in the utility model can be purchased from the market or obtained by private customization.
[0023] like Figures 2 to 4 As shown: a temperature test device for enzyme activity test, comprising a test box 1, a plurality of test chambers 5 distributed in a rectangular array are provided on the surface of the test box 1, a bottom heating plate 6 is fixed to the bottom surface of the test chamber 5, a rotating seat 7 is rotatably installed on the outer side of the bottom heating plate 6, a rotating gear 8 is fixed to the outer surface of the rotating seat 7, a driving gear 11 is meshed and connected to one side of the rotating gear 8, a driving motor 18 is installed below the driving gear 11, the output shaft end of the driving motor 18 is fixed to the driving gear 11, a plurality of symmetrically distributed rotating plates 9 are installed on the inner side of the rotating seat 7, a rotating plate hot plate 14 is inlaid on the inner surface of the rotating plate 9, the bottom end of the rotating plate 9 is rotatably connected to the rotating seat 7 by an axle pin, and the rotating seat 7 is provided with a rotating plate hot plate 14. A wire metal ring 4 is inlaid on the outer surface of the bottom end, and a conductive block 19 is fitted on the outer side of the wire metal ring 4. A storage power supply 13 is installed below the rotating gear 8. The storage power supply 13 is connected to the driving motor 18 and the conductive block 19 by wires. The wire metal ring 4 is connected to the rotating plate hot plate 14 and the bottom heating plate 6 by wires. The storage power supply 13 is used to supply power to the driving motor 18 and the conductive block 19, and after the conductive block 19 is energized, the current will be transmitted to the wire metal ring 4 fitted thereto, and the current will be transmitted to the rotating plate hot plate 14 and the bottom heating plate 6 through the wire metal ring 4 connecting the wires, so that the bottom heating plate 6 and the rotating plate hot plate 14 are energized to generate heat to heat the shaking bottle, so that the experiment can proceed smoothly.
[0024] A plurality of symmetrical electric telescopic rods 10 are fixed on the inner wall of the rotating seat 7, and the number of the electric telescopic rods 10 is consistent with the number of the rotating plates 9. A lifting plate 12 is fixed to the movable end of the electric telescopic rod 10, and the lifting plate 12 is slidably connected to the rotating seat 7. Two connecting plates 15 are fixed to the outer surface of the rotating plate 9, and one end of the lifting plate 12 is located between the two connecting plates 15. A connecting pin 17 is fixed to the end of the lifting plate 12 located between the two connecting plates 15, and a connecting slide 16 is provided on the connecting plate 15. The connecting slide The two ends of 16 penetrate the side wall of the lifting plate 12 and slide and snap into the connecting slide 16. The end of the connecting pin 17 is flush with the outer wall of the connecting plate 15. The lifting plate 12, the connecting pin 17, and the connecting plate 15 are used to connect the rotating plate 9 and the electric telescopic rod 10. When the electric telescopic rod 10 pushes the lifting plate 12 to move up or down, the connecting pin 17 at the end of the lifting plate 12 will slide in the connecting slide 16, changing the tilt state of the rotating plate 9, which is convenient for placing the shaking bottle, or using the rotating plate hot plate 14 to heat the shaking bottle. When the electric telescopic rod is working, the movable end of the electric telescopic rod moves linearly. Since the lifting plate 12 is fixed to the movable end of the electric telescopic rod 10, the electric telescopic rod 10 will be able to push 12 to move along the end direction of 10 when it is started.
[0025] like Figure 1 and Figure 2 As shown, a heat preservation plate 2 is detachably installed on the top of the test chamber 5, and two symmetrically distributed separation blocks 3 are fixed on the upper surface of the heat preservation plate 2. The top of the separation block 3 is bent in a U shape toward the edge of the heat preservation plate 2. The heat preservation plate 2 can be easily taken out by using the separation block 3 on the surface of the heat preservation plate 2, so that the shaking bottle can be conveniently placed in the test chamber 5 or taken out from the test chamber 5.
[0026] Working principle: First, the staff puts the test enzyme in different shaking bottles and shakes them well, then removes the insulation board 2 from the top of the test chamber 5, and puts the shaking bottle containing the enzyme in the test chamber 5, then starts the electric telescopic rod 10 to drive the lifting plate 12 to move downward, and when the lifting plate 12 moves downward, the connecting pin 17 will slide inside the connecting slide groove 16, and then the top of the rotating plate 9 under the assembly of the lifting plate 12 will tilt toward the shaking bottle until the lifting plate 12 drops to the lowest point. At this time, the rotating plate 9 and the rotating plate hot plate 14 are parallel to the outer wall of the shaking bottle, and the insulation board 2 is put back to the top of the test chamber 5, and then the storage power supply 13 is used to power the driving motor 18 and the conductive block 19. After the conductive block 19 is energized The current is transmitted to the wire metal ring 4, and the wire metal ring 4 transmits the power to the bottom heating plate 6 and the rotating plate hot plate 14 through the guide. The bottom heating plate 6 and the rotating plate hot plate 14 generate heat after being energized and transfer the heat to the shaking flask to heat the enzyme in the shaking flask. While the rotating plate hot plate 14 heats the shaking flask, the driving motor 18 can be started to drive the driving gear 11 and the rotating gear 8 to rotate. At this time, the rotating gear 8 will drive the rotating seat 7 and the rotating plate 9 to rotate around the axis of the bottom heating plate 6, so that the rotating plate hot plate 14 makes a circular motion on the outside of the shaking flask at a uniform speed, which can ensure that the rotating plate hot plate 14 parallel to the outer wall of the shaking flask and making a circular motion heats the shaking flask evenly, thereby ensuring the accuracy of the test data.
[0027] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.
Claims
1. A temperature test device for enzyme activity testing, comprising a test chamber (1), characterized in that: The surface of the test box (1) is provided with a plurality of test chambers (5) distributed in a rectangular array, a bottom heating plate (6) is fixed to the bottom surface of the test chamber (5), a rotating seat (7) is rotatably mounted on the outer side of the bottom heating plate (6), a rotating gear (8) is fixed to the outer surface of the rotating seat (7), one side of the rotating gear (8) is meshingly connected with a driving gear (11), a driving motor (18) is mounted below the driving gear (11), an output shaft end of the driving motor (18) is fixed to the driving gear (11), a plurality of symmetrically distributed rotating plates (9) are mounted on the inner side of the rotating seat (7), a rotating plate heating plate (14) is inlaid on the inner surface of the rotating plate (9), the bottom end of the rotating plate (9) is rotatably connected to the rotating seat (7) by means of an axle pin, a wire metal ring (4) is inlaid on the outer surface of the bottom end of the rotating seat (7), a conductive block (19) is fitted on the outer side of the wire metal ring (4), and a storage power supply (13) is mounted below the rotating gear (8).
2. A temperature test device for enzyme activity testing according to claim 1, characterized in that: The storage power source (13) is connected to the drive motor (18) and the conductive block (19) by means of wires, and the wire metal ring (4) is connected to the rotating plate heating plate (14) and the bottom heating plate (6) by means of wires.
3. A temperature test device for enzyme activity testing according to claim 1, characterized in that: A plurality of symmetrical electric telescopic rods (10) are fixed on the inner wall of the rotating seat (7), and the number of the electric telescopic rods (10) is the same as the number of the rotating plates (9).
4. A temperature test device for enzyme activity testing according to claim 3, characterized in that: A lifting plate (12) is fixed to the movable end of the electric telescopic rod (10), the lifting plate (12) is slidably connected to the rotating seat (7), two connecting plates (15) are fixed to the outer surface of the rotating plate (9), and one end of the lifting plate (12) is located between the two connecting plates (15).
5. A temperature test device for enzyme activity testing according to claim 4, characterized in that: A connecting pin (17) is fixedly passed through the end of the lifting plate (12) located between the two connecting plates (15), and a connecting sliding groove (16) is passed through the connecting plate (15).
6. A temperature test device for enzyme activity testing according to claim 5, characterized in that: Both ends of the connecting slide groove (16) penetrate the side wall of the lifting plate (12) and are slidably engaged in the connecting slide groove (16), and the end of the connecting pin (17) is flush with the outer wall of the connecting plate (15).
7. A temperature test device for enzyme activity testing according to claim 1, characterized in that: A heat preservation plate (2) is detachably mounted on the top of the test chamber (5); two symmetrically distributed separation blocks (3) are fixed to the upper surface of the heat preservation plate (2); and the tops of the separation blocks (3) are bent in a U shape toward the edge of the heat preservation plate (2).