Device for rapidly detecting particulate matters in trace liquid

Abstract

The utility model discloses a device for rapidly detecting particulate matters in trace liquid. The device comprises a laser light source, a sample bin, a sample tube, a photoelectric detection system and a data processing system, the sample tube is a transparent container used for placing a sample, the sample bin is provided with a containing cavity used for installing the sample tube, the laser light source is used for emitting laser and irradiating the sample in the sample tube, the sample bin is of a non-transparent structure, the sample bin is provided with a penetrating part matched with a laser path, and the penetrating part is of a transparent window or a hollow structure. The photoelectric detection system is used for converting scattered light signals generated after the sample is irradiated by the laser into electric signals, the photoelectric detection system is connected with the data processing system, and the data processing system is used for processing and calculating the electric signals. According to the utility model, scattered light is generated by irradiating particulate matters in a sample through laser, scattered light signals are converted into electric signals by the photoelectric detection system, and then signal processing and calculation are carried out, so that the sizes of the particulate matters in the sample can be accurately and efficiently obtained.

Description

Technical Field

[0001] The utility model relates to the technical field of particle detection in liquid, in particular to a device for rapidly detecting particles in trace liquid. Background Art

[0002] Proteomics is a science that studies the composition and function of all proteins in organisms. Through the study of proteomics, we can gain a deeper understanding of the mechanisms of metabolic regulation, signal transduction, cell function, and the occurrence and development of related diseases, which is of great significance to scientific research.

[0003] In the study of proteomics, mass spectrometry is one of the most commonly used and powerful tools. When using mass spectrometry to analyze proteomics samples, the samples are usually separated using a nanoliter liquid phase system and then detected by high-resolution mass spectrometry. Because the samples are precious, their volume is usually small, only 8-20 microliters. Before the sample enters the instrument, it is necessary to remove particles from the sample to avoid clogging the instrument or capillary column. Filtration is one of the commonly used methods, but for trace samples, filtration will cause a large amount of sample loss and is therefore not applicable. Centrifugation to obtain supernatant is a common method for processing trace liquid samples, but this method also has some problems. Due to the small amount of sample, the risk of aspirating the bottom particles is high, resulting in particulate impurities in the supernatant, which will clog the capillary column, causing it to be frequently damaged and replaced. In addition, capillary columns have specific design characteristics and manufacturing process limitations, so they are relatively expensive, which increases the cost of use and maintenance.

[0004] Therefore, there is an urgent need for a device that can quickly detect particles in trace amounts of liquid to promptly discover and solve problems during the sample preparation stage. Such a device will help reduce the use and maintenance costs of mass spectrometry experiments and simultaneously improve analysis efficiency and the accuracy of mass spectrometry results.

[0005] There are two main detection technologies currently available:

[0006] One is to observe with the naked eye whether there are particles in the sample. However, particles are small in size, few in number, and weak in strength. Naked eye observation often has the risk of subjective judgment, and it is impossible to accurately detect particles larger than 0.22 microns, so it cannot meet existing needs. In addition, particles are colorless in most cases, making them difficult to detect by visual observation, and they are prone to misjudgment in practical applications. Therefore, a device that can accurately and objectively detect trace liquid particles is needed.

[0007] Another method is to use a traditional dynamic light scattering instrument to detect particles in the sample. However, this method requires a large amount of sample and needs to transfer the sample to a specific sample pool. The transfer process will make the sample smaller, and the feasibility of the technical solution is poor. Therefore, a more efficient and rapid detection method is needed to detect trace liquid particles. Utility Model Content

[0008] In view of the technical problems existing in the prior art, the purpose of the utility model is to provide a device for quickly detecting particles in trace amounts of liquid, which is based on the principles of Brownian motion and dynamic light scattering and can objectively and efficiently detect particles in liquid.

[0009] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a device for rapidly detecting particles in trace amounts of liquid, comprising a laser light source, a sample chamber, a sample tube, a photoelectric detection system and a data processing system.

[0010] The sample tube is a transparent container for placing samples. The sample chamber is provided with a cavity for installing the sample tube. The laser light source is used to emit laser and irradiate the sample in the sample tube. The sample chamber is a non-transparent structure. A penetration portion adapted to the laser path is opened on the sample chamber. The penetration portion is a transparent window or a hollow structure.

[0011] The photoelectric detection system is used to convert the scattered light signal generated by the sample after being irradiated by laser into an electrical signal. The photoelectric detection system is connected to the data processing system, and the data processing system is used to process and calculate the electrical signal.

[0012] With this structure, the sample chamber can be used to fix the sample tube, effectively preventing the sample tube from shaking during the detection process and interference from external light. Under the irradiation of the laser, the particles in the trace liquid in the sample tube will produce scattered light. The photoelectric detection system can directly detect the scattered light signal and convert it into an electrical signal. The signal is processed and calculated by the data processing system to obtain the size of the particles in the sample, which is convenient and efficient.

[0013] As a preference, a device for rapidly detecting particles in trace amounts of liquid further comprises a microscopic magnification system, which receives scattered light and allows an operator to observe the morphology of the particles in the sample.

[0014] As a preference, the position of the microscope magnification system corresponds to scattered light at a scattering angle of 90°.

[0015] As a preferred embodiment, the sample chamber comprises a chamber body and a chamber cover, wherein the chamber cover is located at the top of the chamber body, and both the chamber body and the chamber cover are black.

[0016] As a preferred embodiment, the chamber body is composed of a lower part and an upper part, both of which are hollow columns, the cross-sectional area of ​​the upper part is larger than the cross-sectional area of ​​the lower part, and the cross-sectional area of ​​the lower part matches the cross-sectional size of the sample tube. The penetration part is a hollow structure, and the penetration part is arranged at the lower part of the chamber body.

[0017] As a preferred embodiment, the upper portion is in the shape of a hollow cube, the side length of the upper portion is 2 cm, and the cross section of the lower portion is a square with a side length of 6 mm.

[0018] As a preference, the photoelectric detection system includes a photoelectric detector and a signal amplifier. The position of the photoelectric detector corresponds to the scattered light at a scattering angle of 60°. The photoelectric detector is used to convert the scattered light signal into an electrical signal, and the signal amplifier is used to amplify the electrical signal.

[0019] As a preference, the laser light source is directly opposite to the penetration portion, so that the laser light emitted by the laser light source is perpendicular to the outer surface of the sample tube.

[0020] As a preferred embodiment, the sample tube includes an upper tube body and a lower tube body, both of which are cylindrical, the lower tube body is connected to the lower end of the upper tube body, the outer diameter of the upper tube body is larger than the outer diameter of the lower tube body, and there is a smooth transition between the upper tube body and the lower tube body.

[0021] As a preferred embodiment, the lower end of the lower tube body is connected to a hemispherical tube bottom.

[0022] In general, the utility model has the following advantages:

[0023] (1) By using this device, only the sample needs to be irradiated with laser to quickly determine whether there are large particles of impurities in the sample, thereby greatly improving the detection efficiency and being efficient and fast.

[0024] (2) This device uses a photoelectric detection system and a data processing system, based on the principles of Brownian motion and light scattering, to determine whether there are large particles through calculation, thereby avoiding the influence of subjective factors. It also uses a microscopic magnification system. By observing the magnified image, the operator can intuitively understand the morphology of the particles, thereby improving the accuracy and reliability of the detection results.

[0025] (3) When using this device, the sample only needs to be placed in a transparent liner tube for measurement. After the measurement, the sample can be directly loaded without secondary transfer. This also avoids possible contamination, loss or adsorption problems during sample transfer, achieving zero loss and zero pollution.

[0026] (4) By using this device, large particles in the sample can be found in advance, avoiding the problem of instrument and chromatographic column clogging, reducing the cost of use and maintenance, and also reducing the instrument failure rate. This will greatly reduce the cost of scientific research and the difficulty of instrument practicality, making subsequent experiments more sustainable and economical. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The figure is a schematic diagram of the structure of a device for rapidly detecting particles in trace amounts of liquid.

[0028] Figure 2 This is the front view of the sample compartment.

[0029] Figure 3 This is the left view of the sample compartment.

[0030] Figure 4 Schematic diagram of the position of the sample chamber, photoelectric detection, and microscopic magnification system in the top-down direction.

[0031] Among them, 1 is a laser light source, 2 is a sample chamber, 3 is a sample tube, 4 is a microscope magnification system, 5 is a photoelectric detector, 6 is a signal amplifier, and 7 is a data processing system. DETAILED DESCRIPTION

[0032] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0033] Embodiment 1

[0034] like Figure 1 to Figure 4 As shown, a device for rapidly detecting particles in trace amounts of liquid includes a laser light source, a sample chamber, a sample tube, a photoelectric detection system, and a data processing system.

[0035] The sample tube is a transparent container for placing samples. The sample chamber is provided with a cavity for installing the sample tube. The laser light source is used to emit laser and irradiate the sample in the sample tube. The sample chamber is a non-transparent structure. A penetration portion adapted to the laser path is opened on the sample chamber. The penetration portion is a transparent window or a hollow structure.

[0036] The photoelectric detection system is used to convert the scattered light signal generated by the sample after being irradiated by laser into an electrical signal. The photoelectric detection system is connected to the data processing system, and the data processing system is used to process and calculate the electrical signal.

[0037] The above-mentioned laser light source, sample chamber, photoelectric detection system, signal amplifier and data processing system are all fixedly arranged.

[0038] A device for rapidly detecting particles in trace amounts of liquid also includes a microscopic magnification system, which receives scattered light and allows operators to observe the morphology of particles in the sample.

[0039] The position of the microscope magnification system corresponds to scattered light at a scattering angle of 90°.

[0040] The photoelectric detection system includes a photoelectric detector and a signal amplifier. The position of the photoelectric detector corresponds to the scattered light at a 60° scattering angle. The photoelectric detector is used to convert the scattered light signal into an electrical signal, and the signal amplifier is used to amplify the electrical signal. The scattered light detection angle can be selected from other angles within the range of 60-120 degrees according to actual conditions.

[0041] The laser wavelength emitted by the laser light source is determined according to the needs and can be selected within the wavelength range of 400-700 nanometers; the distance between the laser and the bottom of the sample chamber can be selected within the range of 1-10 mm to meet the measurement needs of more samples.

[0042] The microscopic magnification system may adopt a microscope, the photoelectric detector may adopt a photodiode, and the signal amplifier may adopt an existing product.

[0043] The data processing system includes a data acquisition module and a data analysis module. Both the data acquisition module and the data analysis module adopt existing products. The data acquisition module is used to record the signal of scattered light, and the data analysis module is used to determine whether there are large particles in the sample and display the test results.

[0044] The sample chamber comprises a chamber body and a chamber cover. The chamber cover is located at the top of the chamber body. Both the chamber body and the chamber cover are black.

[0045] The sample bin is pure black throughout and can be made of black material or painted black on the surface.

[0046] The chamber body is composed of a lower part and an upper part, both of which are hollow columns, the cross-sectional area of ​​the upper part is larger than the cross-sectional area of ​​the lower part, and the cross-sectional area of ​​the lower part matches the cross-sectional size of the sample tube. The penetration part is a hollow structure, and the penetration part is arranged at the lower part of the chamber body.

[0047] The upper part is in the shape of a hollow cube with a side length of 2 cm, and the cross section of the lower part is a square with a side length of 6 mm.

[0048] The laser light source is directly facing the penetration portion, so that the laser light emitted by the laser light source is perpendicular to the outer surface of the sample tube, thereby ensuring that the laser light passes through the sample.

[0049] The sample tube includes an upper tube body and a lower tube body, both of which are cylindrical. The lower tube body is connected to the lower end of the upper tube body. The outer diameter of the upper tube body is larger than that of the lower tube body, and there is a smooth transition between the upper tube body and the lower tube body.

[0050] The lower end of the lower tube body is connected with a hemispherical tube bottom.

[0051] When the above device is in use, green light with a wavelength of 532nm is used as the laser light source, which passes through the sample vertically at a distance of 3mm from the bottom of the sample tube. When there are particulate matter in the trace liquid sample, the particulate matter will scatter part of the green light. The microscope magnification system located at a 90° scattering angle can directly amplify the sample tube and the scattered light, and the operator can intuitively and roughly see the particle situation of the sample. The photoelectric detector at the 60° position directly acquires the scattered light signal and converts the light signal into an electrical signal. The signal amplifier further amplifies the electrical signal to improve the sensitivity and accuracy of the detection. Finally, the amplified signal is analyzed by the data processing system to analyze the change law of the scattered light signal over time, and the autocorrelation function of the sample scattered light signal is calculated. According to the autocorrelation function equation: g (1) (τ) = exp(-2Dq 2 τ), where τ is the delay time, D is the translational diffusion coefficient, and q is the scattering wave loss. The translational diffusion coefficient D of the particles is analyzed. According to the Stokes-Einstein equation: D = κT / (6ΠηR h ), where D is the translational diffusion coefficient of the particle, κ is the Boltzmann constant, T is the absolute temperature, η is the solvent viscosity, and R h The hydrodynamic radius is used to calculate the particle size. Finally, the particle size calculation results are displayed in the data processing system, including the average size, maximum size and other parameters of the particles, for further analysis and application.

[0052] Embodiment 2

[0053] The difference between this embodiment and the first embodiment is that when used, considering that the actual use is concerned about whether the sample contains particles larger than 0.22 microns, therefore, in this embodiment, 0.22 micron standard particles are used as samples, and a laser with a wavelength of 532 nanometers is used as a light source. When the laser passes through the standard particle sample, scattered light will be generated, and the photodetector records the change pattern of the scattered light signal over time. Due to the existence of Brownian motion, the scattered light generated by the particles appears as light fluctuations. The larger the particle size, the lower the light fluctuation frequency per unit time, and the smaller the particle size, the higher the light fluctuation frequency. It can be seen from the Stokes-Einstein equation that the fluctuation of the particles is related to the particle size, but has nothing to do with the particle concentration. In the experiment, the scattered light fluctuation frequency of the 0.22 micron standard particles was measured 10 times in succession, and the average fluctuation frequency F was calculated. m Subsequently, the standard particles were replaced with conventional proteomics samples, and the qualification of the samples was evaluated by measuring the scattered light fluctuation frequency. m When the scattered light fluctuation frequency is less than F m The result is displayed as "failed".

[0054] The above embodiments are preferred implementation modes of the utility model, but the implementation modes of the utility model are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principle of the utility model should be equivalent replacement methods and are included in the protection scope of the utility model.

Claims

1. A device for rapidly detecting particles in trace amounts of liquid, characterized in that: It includes laser light source, sample chamber, sample tube, photoelectric detection system and data processing system; The sample tube is a transparent container for placing samples, the sample chamber is provided with a cavity for installing the sample tube, the laser light source is used to emit laser and irradiate the sample in the sample tube, the sample chamber is a non-transparent structure, and a penetration portion adapted to the laser path is opened on the sample chamber, and the penetration portion is a transparent window or a hollow structure; The photoelectric detection system is used to convert the scattered light signal generated by the sample after being irradiated by laser into an electrical signal. The photoelectric detection system is connected to the data processing system, and the data processing system is used to process and calculate the electrical signal.

2. A device for rapidly detecting particles in a trace amount of liquid according to claim 1, characterized in that: The device also includes a microscope magnification system, which receives scattered light and allows an operator to observe the morphology of particles in the sample.

3. A device for rapidly detecting particles in a trace amount of liquid according to claim 2, characterized in that: The position of the microscope magnification system corresponds to scattered light at a scattering angle of 90°.

4. A device for rapidly detecting particles in a trace amount of liquid according to claim 1, characterized in that: The sample chamber includes a chamber body and a chamber cover. The chamber cover is located at the top of the chamber body. Both the chamber body and the chamber cover are black.

5. A device for rapidly detecting particles in a trace amount of liquid according to claim 4, characterized in that: The bin body is composed of a lower part and an upper part, both of which are hollow columns, the cross-sectional area of ​​the upper part is larger than that of the lower part, and the cross-sectional area of ​​the lower part matches the cross-sectional size of the sample tube; the penetration part is a hollow structure, and the penetration part is arranged at the lower part of the bin body.

6. A device for rapidly detecting particles in a trace amount of liquid according to claim 5, characterized in that: The upper part is in the shape of a hollow cube with a side length of 2 cm, and the cross section of the lower part is a square with a side length of 6 mm.

7. A device for rapidly detecting particles in trace amounts of liquid according to claim 1, characterized in that: The photoelectric detection system includes a photoelectric detector and a signal amplifier. The position of the photoelectric detector corresponds to the scattered light at a scattering angle of 60°. The photoelectric detector is used to convert the scattered light signal into an electrical signal, and the signal amplifier is used to amplify the electrical signal.

8. A device for rapidly detecting particles in a trace amount of liquid according to claim 1, characterized in that: The laser light source faces the penetration portion, so that the laser light emitted by the laser light source is perpendicular to the outer surface of the sample tube.

9. A device for rapidly detecting particles in a trace amount of liquid according to claim 1, characterized in that: The sample tube includes an upper tube body and a lower tube body, both of which are cylindrical. The lower tube body is connected to the lower end of the upper tube body. The outer diameter of the upper tube body is larger than that of the lower tube body, and there is a smooth transition between the upper tube body and the lower tube body.

10. A device for rapidly detecting particles in trace amounts of liquid according to claim 9, characterized in that: The lower end of the lower tube body is connected with a hemispherical tube bottom.

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