Gradient cooling box
By setting a ventilation device on the lid of the gradient cooling box to balance the air pressure, the problem of low air pressure in the box is solved, making it difficult to open the lid, and convenient operation and efficient freezing are achieved.
Patent Information
- Application Number
- CN202420888581.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-26
- Publication Date
- 2024-12-27
- Estimated Expiration
- 2034-04-26
AI Technical Summary
When the existing gradient cooling box is taken out from a -80°C refrigerator, the air pressure inside the box is low, forming a pressure difference with the external atmospheric pressure, which makes it difficult to open the cover.
A gradient cooling box is designed, including a ventilation device. By setting a ventilation device on the cover body, when pressing the pressing tablet downward, the movable rod pushes the plug downward, so that the inside of the box body is connected with the outside air, balances the air pressure, and facilitates opening the cover. and improve the sealing effect through springs.
It is achieved to facilitate opening the lid when removing the gradient cooling box, ensure the quality of freezing, and improve the freezing effect through the sealing structure.
Smart Images
Figure CN222236446U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of biological experimental tools, in particular to a gradient cooling box. Background Art
[0002] In the biopharmaceutical industry, prepared biological products, especially cells, need to be stored in a -196°C deep cryogenic environment to maintain their biological activity, so that they can be taken out of the -196°C deep cryogenic environment when needed in the future and used to prepare cells for culture. Therefore, the quality of the cryopreservation will directly determine the biological activity and biological characteristics of the cryopreserved cells.
[0003] In the process of freezing cells, it is necessary to cool them slowly, because if the cells are directly placed from normal temperature to -196℃ deep cryogenic temperature, the water in the cells will produce ice crystal effect, resulting in cell puncture. At present, gradient cooling boxes are commonly used to achieve the slow cooling function. The gradient cooling box contains isopropanol. After the cells are loaded into the cryopreservation tube, they are transferred to the gradient cooling box, and then the gradient cooling box is placed in a -80℃ refrigerator, so that the gradient cooling can be achieved slowly to -80℃, and then transferred to -196℃ liquid nitrogen for storage to avoid cell puncture.
[0004] However, when the existing gradient cooling box is taken out of the -80°C refrigerator, the lid is difficult to open due to the low air pressure inside the box and the pressure difference with the external atmospheric pressure.
[0005] Therefore, we need to design a gradient cooling box to solve the above problems. Utility Model Content
[0006] The utility model aims to solve the problem that when the existing gradient cooling box is taken out of a -80°C refrigerator, the lid is difficult to open due to the low air pressure inside the box and the pressure difference with the external atmospheric pressure.
[0007] According to one aspect of the utility model, a gradient cooling box is provided, comprising a box body, a cover body and a pipe groove, wherein the cover body is arranged on the top of the box body, the pipe groove is arranged in the box body, a plurality of placement grooves are arranged at the bottom of the pipe groove, a through hole is opened at the center position of the cover body, a connecting tube is arranged in the through hole, the connecting tube is fixedly connected to the cover body, and a ventilation device is fixedly connected in the connecting tube. When in use, the cover is removed from the box body, the tube groove is removed, and isopropanol solution is poured into the box body to assist in cooling the cells. The cryopreservation tubes containing the cells are then placed in the placement groove at the bottom of the tube groove. The tube groove is then returned to the box body, and the cover is re-closed. The gradient cooling box is then placed in a -80°C refrigerator, and the temperature is gradually reduced to -80°C. The gradient cooling box is then taken out, the cover is opened, and the cryopreservation tubes are transferred to -196°C liquid nitrogen for storage. When the gradient cooling box is taken out of the -80°C refrigerator, the ventilation device on the cover body can connect the box body with the external air, so that the air pressure inside the box body is balanced with the external air pressure, making it easy to open the cover body.
[0008] In some embodiments, the ventilation device includes a sealing block, the sealing block is fixedly connected in the connecting cylinder, the top of the sealing block is fixedly connected to a limiting plate, a ventilation cavity is provided inside the limiting plate, a connecting cavity is provided inside the sealing block, the connecting cavity is connected to the ventilation cavity, a ventilation shell connected to the ventilation cavity is embedded on the top of the limiting plate, ventilation holes are provided on both sides of the ventilation shell, a pressing piece is provided in the middle of the top of the ventilation shell, a movable rod is fixedly connected to the bottom of the pressing piece, a plug is fixedly connected to the bottom of the movable rod, a partition net is provided at the bottom of the connecting cavity, and a spring is provided between the bottom of the plug and the partition net. Therefore, the utility model provides a ventilation device on the cover body, and when the pressing piece is pressed downward, the movable rod can push the plug downward, so that the box body is connected with the external air, and the external air enters the box body from the vent hole, so that the air pressure in the box body is balanced with the external air pressure, so that the cover body is easy to open.
[0009] In some embodiments, the connecting cavity includes a cylindrical cavity and a conical cavity that are connected, the partition net is arranged at the bottom of the conical cavity, and the plug is conical and used to block the cylindrical cavity. Therefore, when the pressing piece is released, the spring can lift the plug and press it against the bottom of the cylindrical cavity, thereby improving the blocking effect of the cylindrical cavity, achieving the sealing of the gradient cooling box, and improving the quality of cryopreservation.
[0010] In some embodiments, the pressing sheet is made of rubber.
[0011] In some embodiments, a placement ring is disposed on the top of the tube groove, and a limiting step that cooperates with the placement ring and abuts against the placement ring is disposed on the top of the inner side of the box body. Thus, the tube groove is placed in the box body in a manner that its placement ring abuts against the limiting step of the box body.
[0012] In some embodiments, a protrusion is provided at the center of the bottom of the tube slot, so that the protrusion is used as a handle, so that the tube slot can be taken out of the box body by grasping the protrusion.
[0013] In some embodiments, the outer side of the cover is provided with anti-skid patterns, thereby increasing the friction between the cover and the operator's palm, making it easier to open the cover.
[0014] In some embodiments, the outer side of the top of the box body is provided with an external thread, the inner side of the cover body is provided with an internal thread, and the box body and the cover body are connected by threads. Thus, the box body and the cover body are threadedly connected, and the connection structure is relatively stable, which can achieve the sealing of the gradient cooling box and improve the quality of cryopreservation.
[0015] In some embodiments, the box body and the cover body are both cylindrical, and the limiting step is a circular step.
[0016] In some embodiments, the material of the box body is transparent polycarbonate, and the material of the cover body and the tube groove is polyethylene. Thus, the box body is made of a transparent material, so that the operator can observe the liquid level of the isopropanol solution in the box body from outside the box body, so as to add the isopropanol solution in time when the isopropanol solution is reduced, so as to avoid cell damage.
[0017] The beneficial effects of the utility model are as follows:
[0018] 1. When in use, remove the cover from the box body, remove the tube groove, pour isopropanol solution into the box body to assist in cooling the cells, then place the cryopreservation tubes containing cells into the placement groove at the bottom of the tube groove, then put the tube groove back into the box body, and re-cover the cover body, then place the gradient cooling box in a -80℃ refrigerator, gradient cool to -80℃, then take out the gradient cooling box, open the cover body, and then transfer the cryopreservation tubes to -196℃ liquid nitrogen for storage; when the gradient cooling box is taken out of the -80℃ refrigerator, the ventilation device on the cover body can connect the box body with the external air, so that the air pressure inside the box body is balanced with the external air pressure, which is convenient for opening the cover body.
[0019] 2. The utility model provides a ventilation device on the cover body. When the pressing plate is pressed downward, the movable rod can push the plug downward, so that the box body is connected with the external air, and the external air enters the box body from the vent hole, so that the air pressure in the box body is balanced with the external air pressure, which facilitates opening the cover body; the spring is in an extruded state. After the pressing plate is released, the spring can lift the plug and press it against the bottom of the columnar cavity, thereby improving the sealing effect of the columnar cavity, realizing the sealing of the gradient cooling box, and improving the freezing quality. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 It is a schematic diagram of the three-dimensional appearance structure of the utility model;
[0021] Figure 2 It is a three-dimensional structural schematic diagram of the box body of the utility model;
[0022] Figure 3 This is a bottom-up three-dimensional structural diagram of the cover body of the utility model;
[0023] Figure 4 It is a three-dimensional structural schematic diagram of the connecting tube of the utility model;
[0024] Figure 5 It is a partial cross-sectional structural schematic diagram of the ventilation device of the utility model;
[0025] Figure 6 It is a three-dimensional structural schematic diagram of the pipe groove of the utility model.
[0026] In the figure: 1. box body; 2. cover body; 3. anti-slip pattern; 4. limit plate; 6. vent hole; 7. pressing plate; 8. vent shell; 9. external thread; 10. limit step; 12. connecting tube; 13. pipe groove; 14. placement ring; 15. placement groove; 16. protrusion; 17. connecting cavity; 1701, cylindrical cavity; 1702, conical cavity; 18, partition net; 19, sealing block; 20, spring; 21, plug; 22, movable rod; 23, vent cavity. DETAILED DESCRIPTION
[0027] The utility model will be further described in detail below in conjunction with the accompanying drawings.
[0028] The following describes the utility model and its implementation methods, which are not restrictive and the actual implementation methods are not limited thereto. In short, if ordinary technicians in this field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention of the utility model, they should all fall within the protection scope of the utility model.
[0029] refer to Figure 1-6The utility model provides a gradient cooling box, including a box body 1, a cover body 2 and a pipe groove 13. The cover body 2 is arranged on the top of the box body 1, the pipe groove 13 is arranged in the box body 1, and a plurality of placement grooves 15 are arranged at the bottom of the pipe groove 13. A through hole is opened at the center of the cover body 2, and a connecting tube 12 is arranged in the through hole. The connecting tube 12 is fixedly connected to the cover body 2, and a ventilation device is fixedly connected in the connecting tube 12. When in use, the cover 2 is removed from the box body 1, the tube groove 13 is removed, and isopropanol solution is poured into the box body 1 to assist in cooling the cells. The cryopreservation tube containing the cells is then placed in the placement groove 15 at the bottom of the tube groove 13, and then the tube groove 13 is returned to the box body 1, and the cover 2 is re-covered. The gradient cooling box is then placed in a -80°C refrigerator and gradiently cooled to -80°C. The gradient cooling box is then taken out, the cover 2 is opened, and the cryopreservation tube is transferred to -196°C liquid nitrogen for storage. When the gradient cooling box is taken out of the -80°C refrigerator, the ventilation device on the cover 2 can connect the box body 1 with the external air, so that the air pressure inside the box body 1 is balanced with the external air pressure, making it easy to open the cover 2.
[0030] The ventilation device includes a sealing block 19, which is fixedly connected in the connecting tube 12. The top of the sealing block 19 is fixedly connected to the limiting plate 4. The limiting plate 4 has a ventilation cavity 23 inside. The sealing block 19 has a connecting cavity 17 inside. The connecting cavity 17 is connected to the ventilation cavity 23. A ventilation shell 8 connected to the ventilation cavity 23 is embedded in the top of the limiting plate 4. Ventilation holes 6 are provided on both sides of the ventilation shell 8. A pressing piece 7 is provided in the middle of the top of the ventilation shell 8. The pressing piece 7 is made of rubber. The bottom of the pressing piece 7 is fixedly connected to a movable rod 22. The bottom of the movable rod 22 is fixedly connected to a plug 21. A partition net 18 is provided at the bottom of the connecting cavity 17. A spring 20 is provided between the bottom of the plug 21 and the partition net 18. The spring 20 is in an extruded state. Specifically, the connecting cavity 17 includes a connected and conical cavity 1702, the partition net 18 is arranged at the bottom of the conical cavity 1702, and the plug 21 is conical and used to block the column cavity 1701. In implementation, by pressing the pressing sheet 7 downward, the movable rod 22 pushes the plug 21 downward, so that the box body 1 is connected with the external air, and the external air enters the box body 1 from the vent 6, so that the air pressure in the box body 1 is balanced with the external air pressure, which is convenient for opening the cover body 2; the spring 20 is in an extruded state, and after releasing the pressing sheet 7, the spring 20 can lift the plug 21 and press it against the bottom of the column cavity 1701, thereby improving the blocking effect of the column cavity 1701, realizing the sealing of the gradient cooling box, and improving the quality of freezing.
[0031] A placement ring 14 is provided at the top of the tube groove 13, and a limiting step 10 is provided at the top of the inner side of the box body 1 to cooperate with the placement ring 14. The tube groove 13 is placed in the box body 1 in such a way that its placement ring 14 abuts against the limiting step 10 of the box body 1. A protrusion 16 is provided at the center of the bottom of the tube groove 13. The protrusion 16 is used as a handle, so that the tube groove 13 can be taken out of the box body 1 by grasping the protrusion 16.
[0032] The outer side of the cover body 2 is provided with anti-skid patterns 3. By providing the anti-skid patterns 3, the friction between the cover body 2 and the operating palm can be increased, so that the cover body 2 can be opened conveniently. The outer side of the top of the box body 1 is provided with an external thread 9, and the inner side of the cover body 2 is provided with an internal thread, and the box body 1 and the cover body 2 are connected by threads. The box body 1 and the cover body 2 are threadedly connected, and the connection structure is relatively stable, which can achieve the sealing of the gradient cooling box and improve the quality of freezing. The box body 1 and the cover body 2 are both cylindrical, and the limiting step 10 is a circular step. The material of the box body 1 is transparent polycarbonate, and the material of the cover body 2 and the pipe groove 13 is polyethylene. The box body 1 is made of transparent material, so that the operator can observe the liquid level of the isopropanol solution in the box body 1 from the outside of the box body 1, so as to add the isopropanol solution in time when the isopropanol solution is reduced to avoid cell damage.
[0033] The working principle of the utility model is as follows: when in use, first remove the cover 2 from the box body 1, and move the tube groove 13 out of the box body 1, then pour isopropanol solution into the box body 1 to assist in cooling the cells, then put the cryotubes containing cells into the placement groove 15 at the bottom of the tube groove 13, then put the tube groove 13 back into the box body 1, and re-cover the cover 2. Then place the gradient cooling box in a -80°C refrigerator, gradient cooling to -80°C, then take out the gradient cooling box, open the cover 2, and then transfer the cryotubes to -196°C liquid nitrogen for storage.
[0034] When the gradient cooling box is taken out of the -80℃ refrigerator, the pressing plate 7 can be pressed downward to make the movable rod 22 push the plug 21 downward, so that the box body 1 is connected with the external air, and the external air enters the box body 1 from the vent 6, so that the air pressure in the box body 1 is balanced with the external air pressure, making it easy to open the cover body 2.
Claims
1. A gradient cooling box, comprising a box body (1), a cover body (2) and a pipe groove (13), wherein the cover body (2) is arranged on the top of the box body (1), the pipe groove (13) is arranged in the box body (1), and a plurality of placement grooves (15) are arranged at the bottom of the pipe groove (13), characterized in that: A through hole is provided at the center of the cover body (2), a connecting tube (12) is arranged in the through hole, the connecting tube (12) is fixedly connected to the cover body (2), and a ventilation device is fixedly connected in the connecting tube (12).
2. The gradient cooling box according to claim 1, characterized in that: The ventilation device comprises a sealing block (19), wherein the sealing block (19) is fixedly connected in the connecting tube (12), the top of the sealing block (19) is fixedly connected to a limiting plate (4), a ventilation cavity (23) is provided inside the limiting plate (4), a connecting cavity (17) is provided inside the sealing block (19), the connecting cavity (17) is connected to the ventilation cavity (23), and a connecting cavity (17) connected to the ventilation cavity (23) is embedded in the top of the limiting plate (4). A ventilation shell (8) is provided, ventilation holes (6) are provided on both sides of the ventilation shell (8), a pressing plate (7) is provided in the middle of the top of the ventilation shell (8), a movable rod (22) is fixedly connected to the bottom of the pressing plate (7), a plug (21) is fixedly connected to the bottom of the movable rod (22), a partition net (18) is provided at the bottom of the connecting cavity (17), and a spring (20) is provided between the bottom of the plug (21) and the partition net (18).
3. The gradient cooling box according to claim 2, characterized in that: The connecting cavity (17) comprises a connected cylindrical cavity (1701) and a conical cavity (1702), the partition net (18) is arranged at the bottom of the conical cavity (1702), and the plug (21) is conical and is used to block the cylindrical cavity (1701).
4. The gradient cooling box according to claim 2, characterized in that: The pressing sheet (7) is made of rubber.
5. The gradient cooling box according to claim 1, characterized in that: A placement ring (14) is provided at the top of the tube groove (13), and a limiting step (10) that cooperates and abuts against the placement ring (14) is provided at the top of the inner side of the box body (1).
6. The gradient cooling box according to claim 1, characterized in that: A protrusion (16) is provided at the center of the bottom of the tube groove (13).
7. The gradient cooling box according to claim 1, characterized in that: The outer side of the cover body (2) is provided with anti-slip grooves (3).
8. The gradient cooling box according to claim 1, characterized in that: The outer side of the top of the box body (1) is provided with an external thread (9), the inner side of the cover body (2) is provided with an internal thread, and the box body (1) and the cover body (2) are connected via threads.
9. The gradient cooling box according to claim 5, characterized in that: The box body (1) and the cover body (2) are both cylindrical, and the limiting step (10) is a circular step.
10. The gradient cooling box according to claim 1, characterized in that: The box body (1) is made of transparent polycarbonate, and the cover body (2) and the tube groove (13) are made of polyethylene.