Sealing cover structure for PCR (polymerase chain reaction) droplet formation

Abstract

The utility model discloses a sealing cover structure for forming PCR (Polymerase Chain Reaction) liquid drops. The sample tube mounting parts are sequentially arranged on the body part at intervals, each sample tube mounting part comprises a clamping part located at the lower end of the body part and a boss part protruding out of the upper end face of the body part, each clamping part is provided with a hollow inner cavity, and a clamping groove allowing a sample tube to be placed in is formed between the periphery of each clamping part and the body part; the lower end of the hollow inner cavity is open, a closed part is formed at the upper end of the hollow inner cavity, a liquid drop gathering groove for gathering liquid drops is formed between the upper end of the closed part and the boss part, and a puncture structure is arranged at the lower end of the closed part. According to the sealing cover structure for forming the PCR liquid drops, provided by the utility model, the detection efficiency and precision can be improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of biochips, and particularly relates to a sealing cover structure used for forming PCR droplets. Background Art

[0002] Biochips used for digital PCR are generally designed with eight or more chip channels for generating and detecting droplets.

[0003] During the detection, the eight-tube is loaded into the droplet generation device, and a certain number of droplets with uniform size and stable shape are generated in combination with biological reagents and biochips, and the generated droplets flow into the eight-tube; the eight-tube containing the droplets is put into the gene amplification instrument for amplification; the amplified eight-tube and the eight-tube rubber cover are assembled and put into the droplet detection device; the biological detection chip is put into the device, the biological chip is pressed down, and a seal is formed through the bottom surface of the biochip and the raised structure of the eight-tube rubber cover; the reagent oil enters the interior of the eight-tube through the microchannel of the biological detection chip under the drive of the device. Since the specific gravity of the reagent oil is greater than that of the droplets, the droplets enter the microchannel of the biological detection chip under the push of the reagent oil, and the fluorescence detection inside the droplets is carried out through the optical system in the device.

[0004] The connection between the rubber cover and the sample tube in the prior art is an open-hole structure for the reagent oil to flow into the sample tube. However, this structure has the following defects: during amplification, the droplets in the sample tube need to be sealed to avoid contamination. After amplification, the rubber cover must be replaced with an open structure to allow the reagent oil to flow into the sample tube through the opening. Therefore, the rubber cover of the sample tube containing the droplets after amplification needs to be replaced before being placed in the detection equipment. During the replacement process, the droplets are exposed to the external environment, which can easily cause the droplets in the tube to be contaminated, affecting the measurement accuracy and results.

[0005] In order to solve the above problems, the present utility model is provided. Utility Model Content

[0006] The utility model aims to provide a sealing cover structure for PCR droplet formation, which can improve detection efficiency and accuracy.

[0007] Based on the above problems, the technical solution provided by the utility model is:

[0008] The sealing cover structure for PCR droplet formation comprises:

[0009] Body part;

[0010] A plurality of sample tube mounting parts are arranged on the main body in sequence and at intervals. The sample tube mounting parts include a clamping part located at the lower end of the main body and a boss part protruding from the upper end surface of the main body. The clamping part has a hollow inner cavity and a clamping groove for inserting the sample tube is provided between the outer periphery and the main body. The lower end of the hollow inner cavity is open and the upper end forms a closed part. A droplet gathering groove for gathering droplets is formed between the upper end of the closed part and the boss part. A puncture structure is provided on the closed part.

[0011] In some embodiments, the puncture structure is an avoidance groove arranged at the lower end of the closing portion.

[0012] In some embodiments, the avoidance groove includes a first groove body and a second groove body arranged vertically, and the size of the second groove body is larger than that of the first groove body.

[0013] In some embodiments, the cross-section of the first trough body is rectangular, and the cross-section of the second trough body is trapezoidal.

[0014] In some embodiments, the fixing portion is tapered from top to bottom.

[0015] In some embodiments, a circle of protrusions protruding outwards is formed on the outer periphery of the main body.

[0016] Compared with the prior art, the advantages of the utility model are:

[0017] 1. By adopting the technical solution of the utility model, a puncture structure is added to the sealing cover structure to ensure the seal during amplification. After amplification, there is no need to replace the sealing cover structure, which prevents the droplets from being contaminated by the external environment, saving material costs and replacement time and labor costs;

[0018] 2. A droplet aggregation groove for aggregating droplets is added to the sealing cover structure. The droplets flowing out of the sample tube are gathered through the droplet aggregation groove, which increases the density of the droplets flowing out, reduces the use of reagent oil, and reduces the detection cost. In actual detection, the distance between droplets is significantly narrowed, the number of droplets detected per unit time is increased, the detection time is shortened, and the detection efficiency is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the description of the embodiments will be briefly introduced below. The drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1This is one of the structural schematic diagrams of an embodiment of the sealing cover structure for PCR droplet formation of the utility model;

[0021] Figure 2 This is the second structural diagram of the embodiment of the utility model;

[0022] Figure 3 for Figure 2 The local enlarged view of point I in the middle;

[0023] Figure 4 This is the third structural diagram of the embodiment of the utility model;

[0024] Figure 5 for Figure 4 Schematic diagram of the AA section;

[0025] Figure 6 This is a schematic diagram of the use state of an embodiment of the utility model;

[0026] Figure 7 for Figure 6 Schematic diagram of the middle BB section;

[0027] Figure 8 for Figure 7 A partial enlarged view of the middle J;

[0028] in:

[0029] 1. Body part;

[0030] 2. Fixing part;

[0031] 3. Boss part;

[0032] 4. Closing part;

[0033] 5. Droplet aggregation tank;

[0034] 6. puncture structure 6-1, first groove portion 6-2, second groove portion;

[0035] 7. Bump;

[0036] 100, droplet detection device 101, droplet inlet 102, droplet observation area 103, test tube; 200, biochip 201, puncture part 300, sample tube. DETAILED DESCRIPTION

[0037] The above scheme is further described below in conjunction with specific examples. It should be understood that these examples are used to illustrate the utility model and are not limited to the scope of the utility model. The implementation conditions used in the examples can be further adjusted according to the conditions of the specific manufacturer, and the implementation conditions not specified are usually the conditions in conventional experiments.

[0038] See also Figures 1 to 5, is a schematic diagram of the structure of an embodiment of the utility model, which provides a sealing cover structure for PCR droplet formation, including a main body 1 and eight sample tube mounting parts arranged on the main body 1, the main body 1 is a long strip structure, and the eight sample tube mounting parts are arranged on the main body 1 in sequence.

[0039] The sample tube mounting portion includes a clamping portion 2 located at the lower end of the main body 1 and a boss portion 3 protruding from the upper end surface of the main body 1. The clamping portion 2 has a hollow inner cavity and a slot for inserting the sample tube 300 between the outer periphery and the main body 1. The lower end of the hollow inner cavity is open and a closed portion 4 is formed at the upper end. A droplet gathering groove 5 for gathering droplets is formed between the upper end of the closed portion 4 and the boss portion 3, and a puncture structure 6 is provided on the closed portion 4.

[0040] like Figures 6 to 8 As shown, the sample tube 300 is clamped in the card slot to achieve a sealed connection between the sample tube 300 and the sealing cover structure, and then the sample tube 300 and the sealing cover structure are installed as a whole on the droplet detection device 100. After the biochip 200 is loaded, the biochip 200 abuts against the boss portion 3 to achieve sealing, and the puncture portion 201 on the biochip 200 extends into the sample tube 300 through the sealing portion 4, so that the reagent oil flows into the sample tube 300.

[0041] Specifically, the puncture structure 6 is an escape groove arranged at the lower end of the closing portion 4, and the escape groove includes a first groove body 6-1 and a second groove body 6-2 arranged up and down, and the size of the second groove body 6-2 is larger than that of the first groove body 6-1, so that the puncture portion 201 can puncture the puncture structure 6. In this example, the cross section of the first groove body 6-1 is rectangular, and the cross section of the second groove body 6-2 is trapezoidal.

[0042] In this example, the fastening portion 2 is tapered from top to bottom to facilitate fastening and fixing of the sample tube 300 .

[0043] In order to facilitate fixing the sealing cover structure on the droplet detection device 100 , a circle of bumps 7 protruding outwards is formed on the outer periphery of the main body 1 , and the bumps 7 are interference-fitted with the droplet detection device 100 to achieve a fixed seal.

[0044] The working principle of the utility model is:

[0045] The sample tube 300 and the sealing cover structure are installed on the droplet detection device 100, and the biochip 200 is installed in the droplet detection device 100. The puncture portion 201 on the biochip 200 punctures the puncture structure 6 on the sealing cover structure and extends into the sample tube 300. The reagent oil enters the sample tube 300 through the microchannel and the puncture portion 201 on the biochip 200. Since the specific gravity of the reagent oil is greater than that of the droplets, the droplets float on the surface of the reagent oil and flow out through the sealing cover. The droplets gather in the droplet gathering groove 5 at the upper end of the sealing cover structure and then flow into the droplet observation area 102, and finally enter the test tube 103 for testing.

[0046] In summary, the sealing cover structure does not need to be replaced after amplification, thereby improving detection efficiency and at the same time increasing droplet outflow density, thereby improving detection accuracy.

[0047] The above examples are only for illustrating the technical concept and features of the utility model, and their purpose is to enable people familiar with the technology to understand the content of the utility model and implement it accordingly, and they cannot be used to limit the protection scope of the utility model. Any equivalent changes or modifications made according to the spirit of the utility model should be included in the protection scope of the utility model.

Claims

1. A sealing cover structure for PCR droplet formation, characterized in that: include: Body part; A plurality of sample tube mounting parts are arranged on the main body in sequence and at intervals. The sample tube mounting parts include a clamping part located at the lower end of the main body and a boss part protruding from the upper end surface of the main body. The clamping part has a hollow inner cavity and a clamping groove for inserting the sample tube is provided between the outer periphery and the main body. The lower end of the hollow inner cavity is open and the upper end forms a closed part. A droplet gathering groove for gathering droplets is formed between the upper end of the closed part and the boss part. A puncture structure is provided on the closed part.

2. The sealing cover structure for PCR droplet formation according to claim 1, characterized in that: The puncture structure is an avoidance groove arranged at the lower end of the closing portion.

3. The sealing cover structure for PCR droplet formation according to claim 2, characterized in that: The avoidance groove includes a first groove body and a second groove body arranged up and down, and the size of the second groove body is larger than that of the first groove body.

4. The sealing cover structure for PCR droplet formation according to claim 3, characterized in that: The cross section of the first trough body is rectangular, and the cross section of the second trough body is trapezoidal.

5. The sealing cover structure for PCR droplet formation according to claim 1, characterized in that: The clamping portion is gradually contracted from top to bottom.

6. The sealing cover structure for PCR droplet formation according to claim 1, characterized in that: A circle of protrusions protruding outwards is formed on the outer periphery of the main body.

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