Cell culture counting plate

Abstract

The utility model discloses a cell culture counting plate which comprises a base and an upper cover, a lower groove is formed in the base, a mixed solution is contained in the lower groove, a sliding groove is formed in the base, one end of the sliding groove is closed, the other end of the sliding groove is open, and a connecting block is correspondingly arranged at the bottom of the upper cover. One side of the upper cover is connected with an extension seat, supporting legs are arranged at the bottom of the extension seat, second pulleys are arranged at the bottoms of the supporting legs, an upper groove is formed in the upper cover, a slide is arranged in the upper groove, and the upper cover is connected to the base in a sliding mode and plays a role in sealing the base; when the cell culture counting plate is used, the mixed solution in the lower groove is dropped on the slide through the dropper, then the slide in the lower groove of the cell culture counting plate is aligned and placed under a lens of an inverted microscope, the number of cells in the sample solution is obtained, and finally the number of cells in the mixed solution is calculated through a counting formula.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture counting plate. Background Art

[0002] In the field of cell biology and medicine, cell culture processing experiments need to be frequently performed, and cell counting and cell culture plates are usually used. In cell culture experiments, it is necessary to count cells before inoculation and to collect cell samples later. The commonly used cell culture plates are composed of a box body and a box cover, which are more convenient to use than a single culture dish. However, they still require multiple handling and sampling during the culture process, and the cells are poorly kept warm, which may cause secondary contamination. In addition, the culture plates currently widely used in laboratories cannot be counted directly, and the box cover needs to be removed for sampling, which is likely to have additional effects on the cells. Utility Model Content

[0003] The utility model aims to provide a cell culture counting plate to solve the problems raised in the above background technology.

[0004] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell culture counting plate, comprising a base and an upper cover, the base being provided with a plurality of lower grooves, a mixed solution being arranged inside the lower grooves, the upper cover being slidably connected to the base to seal the base, the base being provided with a slide groove, one end of the slide groove being closed, and the other end of the slide groove being open, a connecting block being correspondingly arranged at the bottom of the upper cover, the connecting block being slidably connected in the slide groove through a first pulley, an extension seat being connected to one side of the upper cover, a support leg being arranged at the bottom of the extension seat, a second pulley being arranged at the bottom of the support leg, an upper groove being provided on the upper cover, a glass slide being arranged inside the upper groove, a sealing cover being hinged on the upper cover, and the sealing cover being used to seal the upper groove.

[0005] Preferably, a protrusion is provided near one side of the base, and protective edges are provided around the bottom of the upper cover. After the upper cover slides out from the opening end of the slide slot, when the protective edge of the upper cover away from the extension seat contacts the protrusion, it limits the upper cover from continuing to slide along the opening end of the slide slot.

[0006] Preferably, a grooved handle is provided on the top of the extension seat.

[0007] Preferably, the sealing cover is provided with a groove.

[0008] Preferably, a gap is provided between two adjacent upper grooves on the upper cover, and the gap is used to support the sealing cover after turning over.

[0009] Compared with the prior art, the beneficial effects of the utility model are:

[0010] The cell culture counting plate comprises a base and an upper cover, wherein the upper cover is slidably connected to the base and seals the base. After the upper cover slides out from the opening end of the slide groove, when the protective edge of the upper cover away from the extension seat contacts the protrusion, it limits the upper cover from continuing to slide along the opening end of the slide groove. The corresponding lower groove is opened by sliding the upper cover on the base, and there is no need to open the upper cover completely, so as to reduce the influence of bacteria in the external gas on the cells. After the upper cover is opened, the sealing cover is opened, and then the mixed solution in the lower groove is dripped onto a glass slide through a dropper, and then the glass slide in the lower groove of the cell culture counting plate is aligned and placed under the lens of an inverted microscope to obtain the number of cells in the sample solution, and finally the number of cells in the mixed solution is calculated through a counting formula. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0012] Figure 2 It is a schematic diagram of the upper cover structure of the utility model;

[0013] Figure 3 It is a schematic diagram of the A structure of the utility model;

[0014] Figure 4 It is a schematic diagram of the base structure of the utility model;

[0015] Figure 5 It is a schematic diagram of the distribution of the protective edge of the utility model.

[0016] In the accompanying drawings, the following are numerals: 1, base; 2, upper cover; 3, lower groove; 4, slide groove; 5, connecting block; 6, first pulley; 7, extension seat; 8, support foot; 9, second pulley; 10, upper groove; 11, glass slide; 12, sealing cover; 13, protrusion; 14, protective edge; 15, groove handle; 16, groove. DETAILED DESCRIPTION

[0017] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. The same components are denoted by the same reference numerals. It should be noted that the words "front", "rear", "left", "right", "upper" and "lower" used in the following description refer to directions in the accompanying drawings, and the words "bottom" and "top", "inner" and "outer" refer to directions toward or away from the geometric center of a specific component, respectively.

[0018] Example: See Figure 1-5The utility model provides a technical solution: a cell culture counting plate, comprising a base 1 and an upper cover 2, wherein the base 1 is provided with a plurality of lower grooves 3, wherein a mixed solution is arranged inside the lower groove 3, wherein the mixed solution comprises PBS and a cell suspension, and the upper cover 2 is slidably connected to the base 1 to seal the base 1, and a slide groove 4 is provided on the base 1, wherein one end of the slide groove 4 is closed, and the other end of the slide groove 4 is open, and a connecting block 5 is correspondingly provided at the bottom of the upper cover 2, wherein the connecting block 5 is slidably connected to the slide groove 4 through a first pulley 6, an extension seat 7 is connected to one side of the upper cover 2, a support leg 8 is provided at the bottom of the extension seat 7, and a second pulley 9 is provided at the bottom of the support leg 8, an upper groove 10 is provided on the upper cover 2, a glass slide 11 is arranged inside the upper groove 10, and a sealing cover 12 is hinged on the upper cover 2, and the sealing cover 12 is used to seal the upper groove 10, and the sealing cover 12 is opened after the upper cover 2 is opened, and then the mixed solution in the lower groove 3 is dripped onto the glass slide 11 through a dropper.

[0019] In this embodiment, a protrusion 13 is provided near one side of the base 1, and a protective edge 14 is provided around the bottom of the upper cover 2. After the upper cover 2 slides out from the open end of the slide groove 4, when the protective edge 14 on the upper cover 2 away from the extension seat 7 contacts the protrusion 13, it plays a limiting role in preventing the upper cover 2 from continuing to slide along the open end of the slide groove 4.

[0020] In this embodiment, a grooved handle 15 is provided on the top of the extension seat 7, which facilitates the extension seat 7 to slide along the slide groove 4 through the grooved handle 15. After the upper cover 2 slides out from the open end of the slide groove 4, when the protective edge 14 of the upper cover 2 away from the extension seat 7 contacts the protrusion 13, the upper cover 2 fully opens the lower groove 3 on the base 1, and the upper cover 2 slides along the closed end of the slide groove 4. When the side of the connecting block 5 close to the closed end of the slide groove 4 contacts the closed end of the slide groove 4, the upper cover 2 fully protects the lower groove 3 on the base 1.

[0021] In this embodiment, a groove 16 is provided on the sealing cover 12 to facilitate turning over the sealing cover 12 through the groove 16 .

[0022] In this embodiment, a gap is provided between two adjacent upper grooves 10 on the upper cover 2 , and the gap is used to support the sealing cover 12 after being turned over, so as to prevent the sealing cover 12 from affecting the adjacent upper grooves 10 after being turned over.

[0023] Working principle: This cell culture counting plate includes a base 1 and an upper cover 2. The upper cover 2 is slidably connected to the base 1 to seal the base 1. After the upper cover 2 slides out from the open end of the slide groove 4, when the protective edge 14 on the upper cover 2 away from the extension seat 7 contacts the protrusion 13, it limits the upper cover 2 to continue to slide along the open end of the slide groove. The upper cover 2 slides on the base 1 to open the corresponding lower groove 3. There is no need to open the upper cover 2 completely, so as to reduce the influence of bacteria in the external gas on the cells. After the upper cover 2 is opened, the sealing cover 12 is opened, and then the mixed solution in the lower groove 3 is dripped onto the glass slide 11 through a dropper, and then the glass slide 11 in the lower groove 3 of this cell culture counting plate is aligned and placed under the lens of an inverted microscope to obtain the number of cells in the sample solution. The mixed solution cell culture counting process is: when the volume of the absorbed cell suspension is constant, the counting number (individuals / mL) n = the number of cells in the sample solution × the dilution multiple × the volume of the cell suspension.

[0024] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

[0025] In the description of the present invention, unless otherwise specified, "multiple" means two or more; the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third" and the like are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0026] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

Claims

1. A cell culture counting plate, characterized in that: The invention comprises a base (1) and an upper cover (2), wherein the base (1) is provided with a plurality of lower grooves (3), wherein a mixed solution is arranged inside the lower grooves (3), wherein the upper cover (2) is slidably connected to the base (1) to seal the base (1), wherein a slide groove (4) is provided on the base (1), wherein one end of the slide groove (4) is closed and the other end of the slide groove (4) is open, wherein a connecting block (5) is correspondingly provided at the bottom of the upper cover (2), wherein the connecting block (5) is slidably connected to the slide groove (4) via a first pulley (6), wherein an extension seat (7) is connected to one side of the upper cover (2), wherein a support leg (8) is provided at the bottom of the extension seat (7), wherein a second pulley (9) is provided at the bottom of the support leg (8), wherein an upper groove (10) is provided with a glass slide (11) inside the upper groove (10), and wherein a sealing cover (12) is hingedly connected to the upper cover (2), wherein the sealing cover (12) is used to seal the upper groove (10).

2. A cell culture counting plate according to claim 1, characterized in that: A protrusion (13) is provided on the base (1) near one side, and a protective edge (14) is provided around the bottom of the upper cover (2). After the upper cover (2) slides out from the opening end of the slide groove (4), the protective edge (14) on the upper cover (2) away from the extension seat (7) contacts the protrusion (13), thereby limiting the upper cover (2) from continuing to slide along the opening end of the slide groove.

3. A cell culture counting plate according to claim 2, characterized in that: The top of the extension seat (7) is provided with a grooved handle (15).

4. A cell culture counting plate according to claim 3, characterized in that: The sealing cover (12) is provided with a groove (16).

5. A cell culture counting plate according to claim 4, characterized in that: A gap is provided between two adjacent upper grooves (10) on the upper cover (2), and the gap is used to support the sealing cover (12) after being turned over.

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