Cell multiplication culture device
By using power parts and shaking components in the cell amplification culturer to achieve uniform mixing between cells and culture medium, the problem of uneven mixing of traditional stirring systems is solved, the efficiency of cell growth and expansion is improved, and the operation convenience is enhanced .
Patent Information
- Application Number
- CN202420930087.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-30
- Publication Date
- 2024-12-27
- Estimated Expiration
- 2034-04-30
AI Technical Summary
The traditional stirring system does not mix the culture medium and cells evenly enough, resulting in some cells being unable to obtain enough nutrients, affecting the growth and reproduction of cells, and even leading to different cell growth rates or partial cell death.
A cell expansion culture device was designed, using power parts to drive the rotating disc and shaking components to drive the shaking of the culture bottle to ensure full mixing of cells and culture medium, improve the efficiency of nutrient transfer and waste discharge speed, and at the same time, it is convenient to use an electric telescopic rod. Control the height of the culture cover to avoid damage caused by excessive opening and closing.
Achieve uniform mixing of cells and culture medium, promote the transfer of nutrients and waste emissions, improve the efficiency of cell growth and expansion, reduce cell aggregation, enhance operation convenience, and avoid the death of cells due to insufficient nutrients. question.
Smart Images
Figure CN222250782U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of cell culture, in particular to a cell amplification culture device. Background Art
[0002] A cell expansion incubator is a device used to culture and expand cells, usually in biomedicine, bioengineering, drug development, etc. The device mainly includes culture dishes or culture bottles, pressure control systems, temperature control systems, humidity control systems, stirring systems, etc. Appropriate equipment can be selected according to different cell types, culture requirements and experimental purposes.
[0003] In the prior art, when a stirring system mixes culture medium and cells, the traditional stirring system does not mix them uniformly, which causes some cells to be unable to obtain sufficient nutrients, thereby affecting the growth and reproduction of cells, which may lead to different cell growth rates or even the death of some cells.
[0004] To this end, the utility model provides a cell expansion culture device. Utility Model Content
[0005] In order to make up for the shortcomings of the existing technology, in order to solve the problem that when the stirring system mixes the culture medium and cells, the traditional stirring system does not mix them evenly, resulting in some cells being unable to obtain sufficient nutrients, thereby affecting the growth and reproduction of cells, which may lead to different cell growth rates or even the death of some cells.
[0006] The technical solution adopted by the utility model to solve its technical problems is: a cell proliferation culture device described in the utility model comprises a culture box, a culture cover is provided on the top of the culture box, a first groove is provided on the culture box, a rotating disk is rotatably connected to the bottom of the first groove through a first rotating shaft, a group of sliding grooves is provided on the top of the rotating disk, a placement tube is slidably connected in a group of sliding grooves, a first rotating rod is fixedly connected to the rotating disk, a group of second fixed rods are fixedly connected to the first rotating rod through a group of first fixed rods, a power piece is provided on the culture cover, the power piece is used to drive the first rotating rod to rotate, a shaking component is provided on the rotating disk, and the shaking component is used to drive the placement tube to slide in the sliding groove.
[0007] Preferably, the power component includes a motor, which is fixed to the top of the culture cover through a pair of L-shaped rods. A driving rod is provided at the output end of the motor. The bottom of the driving rod is rotatably connected to the top of the culture cover, and the bottom of the driving rod passes through the bottom of the culture cover. A locking block is fixed to the bottom of the driving rod.
[0008] Preferably, the shaking assembly includes a connecting unit and an eccentric block, a group of the eccentric blocks are rotatably connected to the rotating disk via a second rotating shaft, a group of the eccentric blocks are provided with a T-shaped groove on the outer circular wall, a group of the T-shaped grooves are slidably connected with a T-shaped rod, and one end of a group of the T-shaped rods is respectively fixed to the outer circular wall of a group of placing cylinders.
[0009] Preferably, the connecting unit comprises a first sprocket, the first sprocket is fixedly connected to the first rotating rod, a group of eccentric blocks are fixedly connected to the top with a second sprocket, and the first sprocket is connected to the second sprocket via a chain transmission.
[0010] Preferably, a group of fixed grooves are provided at the bottom of the incubator, a group of electric telescopic rods are fixedly connected to the bottom of the fixed grooves, and a group of electric telescopic ends are fixedly connected to the bottom of the incubation cover.
[0011] Preferably, a group of ventilation holes are opened on the culture cover, and a group of rubber plugs are fixedly connected to the top of the culture cover.
[0012] The beneficial effects of the utility model are as follows:
[0013] 1. The cell expansion culture device described in the utility model can drive the culture bottle to rotate when the rotating disk is driven by the power part, so that the cells in the culture bottle and the culture medium are fully and evenly mixed. The shaking component drives the culture bottle to shake, which can promote the material exchange between the culture medium and the cells, improve the transfer efficiency of nutrients and the discharge rate of waste, which is beneficial to the growth and expansion of cells, and can reduce cell aggregation, thereby solving the problem that the traditional stirring system does not mix evenly, resulting in some cells unable to obtain enough nutrients, thereby affecting the growth and reproduction of cells, which may lead to different cell growth rates or even the death of some cells.
[0014] 2. The cell expansion culture device described in the utility model can greatly improve convenience by raising and lowering the culture cover through a set of electric telescopic rods. When the culture cover is opened, the height of the culture cover can be accurately controlled to support the culture cover, thereby facilitating the staff to operate the culture cells and avoiding damage to the incubator and the culture cover due to excessive opening and closing. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] The utility model will be further described below in conjunction with the accompanying drawings.
[0016] Figure 1 It is the front view of the utility model;
[0017] Figure 2 It is a three-dimensional diagram of the utility model;
[0018] Figure 3 It is a schematic diagram of the rotating disk in the utility model;
[0019] Figure 4 It is a schematic diagram of the chain in the utility model;
[0020] Figure 5 It is a schematic diagram of the explosion of the T-shaped rod in the utility model;
[0021] In the figure: 1. incubator; 2. incubator cover; 3. first groove; 4. rotating disk; 5. sliding groove; 6. placement cylinder; 7. first rotating rod; 8. second fixed rod; 9. motor; 10. driving rod; 11. positioning block; 12. eccentric block; 13. T-shaped slide groove; 14. T-shaped rod; 15. first sprocket; 16. second sprocket; 17. chain; 18. fixed groove; 19. electric telescopic rod; 20. ventilation hole; 21. rubber plug. DETAILED DESCRIPTION
[0022] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.
[0023] like Figures 1 to 5 As shown, a cell proliferation culture device described in an embodiment of the utility model comprises a culture box 1, a culture cover 2 is provided on the top of the culture box 1, a first groove 3 is provided on the culture box 1, a rotating disk 4 is rotatably connected to the bottom of the first groove 3 through a first rotating shaft, a group of sliding grooves 5 are provided on the top of the rotating disk 4, a group of the sliding grooves 5 are slidably connected to a placement tube 6, a first rotating rod 7 is fixedly connected to the rotating disk 4, a group of second fixed rods 8 are fixedly connected to the first rotating rod 7 through a group of first fixed rods, a power piece is provided on the culture cover 2, and the power piece is used to drive the first rotating rod 7 to rotate, and a shaking component is provided on the rotating disk 4, and the shaking component is used to drive the placement tube 6 It slides in the sliding groove 5. When working, by placing the culture bottle in the placing cylinder 6, the power part can drive the rotating disk 4 to rotate and drive the culture bottle to rotate, so that the cells in the culture bottle and the culture medium are fully and evenly mixed. The shaking component drives the culture bottle to shake, which can promote the material exchange between the culture medium and the cells, improve the transfer efficiency of nutrients and the discharge rate of waste, which is beneficial to the growth and expansion of cells, and can reduce cell aggregation, so as to solve the problem that the traditional stirring system is not mixed evenly, resulting in some cells unable to obtain enough nutrients, thereby affecting the growth and reproduction of cells, which may lead to different cell growth rates or even the death of some cells.
[0024] The power component includes a motor 9, which is fixedly connected to the top of the culture cover 2 through a pair of L-shaped rods. A driving rod 10 is provided at the output end of the motor 9. The bottom of the driving rod 10 is rotatably connected to the top of the culture cover 2, and the bottom of the driving rod 10 passes through the bottom of the culture cover 2. A locking block 11 is fixedly connected to the bottom of the driving rod 10. When working, the motor 9 is fixed to the cover body, so that when the motor 9 is set in the first groove 3, it can be avoided that the normal operation of the motor 9 is affected, and the space in the first groove 3 can be saved. The locking block 11 can be set to lock a group of second fixed rods 8, so that the setting of the locking block 11 and a group of second fixed rods 8 can drive the first rotating rod 7 to rotate, thereby driving the rotating disk 4 to rotate.
[0025] The shaking assembly includes a connecting unit and an eccentric block 12. A group of the eccentric blocks 12 are rotatably connected to the rotating disk 4 through a second rotating shaft. A group of the eccentric blocks 12 are provided with a T-shaped groove 13 on the outer circular wall. A group of the T-shaped groove 13 is slidably connected with a T-shaped rod 14. One end of a group of the T-shaped rod 14 is respectively fixed to the outer circular wall of a group of placement tubes 6. When working, the first rotating rod 7 is set to drive a group of eccentric blocks 12 to rotate through the connecting unit, so that the T-shaped rod 14 sliding in the T-shaped groove 13 and the placement tube 6 sliding in the sliding groove 5 can cooperate, so that the placement tube 6 can shake while rotating, thereby improving the mixing efficiency of the culture medium and the cells, and allowing the culture medium and the cells to be evenly and fully mixed, so that the cells can fully multiply and grow. The placement tube 6 sliding in the sliding groove 5 cannot rotate.
[0026] The connecting unit includes a first sprocket 15, which is fixedly connected to the first rotating rod 7. A group of eccentric blocks 12 are fixedly connected to the top with a second sprocket 16. The first sprocket 15 and the second sprocket 16 are connected through a chain 17. When working, the first sprocket 15 drives the chain 17 to drive, thereby driving a group of second sprockets 16 to rotate, so that when the first sprocket 15 rotates, it can also drive a group of second sprockets 16 to rotate, and drive the eccentric block 12 to rotate, thereby driving the placement tube 6 to shake, and the culture bottle in the placement tube 6 to shake, so that the culture medium and cells are fully and evenly mixed.
[0027] A group of fixed grooves 18 are provided at the bottom of the incubator 1, and an electric telescopic rod 19 is fixedly connected to the bottom of the fixed grooves 18. A group of telescopic ends of the electric telescopic rods are fixedly connected to the bottom of the culture cover 2. When working, the culture cover 2 is raised and lowered by a group of electric telescopic rods 19, which can greatly improve the convenience. When opening the culture cover 2, the height of the culture cover 2 can be accurately controlled to support the culture cover 2, thereby facilitating the staff to operate the cultured cells and avoiding damage to the incubator 1 and the culture cover 2 during excessive opening and closing.
[0028] A group of ventilation holes 20 are provided on the culture cover 2, and a group of rubber plugs 21 are fixedly connected to the top of the culture cover 2. When working, the group of ventilation holes 20 provided on the culture cover 2 and the group of rubber plugs 21 fixedly connected can control the gas exchange in the incubator 1 to ensure the balance of gas components such as oxygen and carbon dioxide in the culture environment and maintain the normal physiological function of the cells.
[0029] Working principle: by placing the culture bottle in the placing cylinder 6, the power part can drive the rotating disk 4 to rotate and drive the culture bottle to rotate, so that the cells in the culture bottle and the culture medium are fully and evenly mixed. The shaking component drives the culture bottle to shake, which can promote the material exchange between the culture medium and the cells, improve the transfer efficiency of nutrients and the discharge rate of waste, which is beneficial to the growth and expansion of cells, and can reduce cell aggregation, thereby solving the problem that the traditional stirring system is not mixed evenly, resulting in some cells unable to obtain enough nutrients, thereby affecting the growth and reproduction of cells, which may cause the cell growth rate to be slow. The problem of even partial cell death is solved by setting the motor 9 fixedly connected to the cover body, so as to avoid affecting the normal operation of the motor 9 when the motor 9 is set in the first groove 3, and the space in the first groove 3 can be saved. The set of the positioning block 11 can position a group of second fixed rods 8, so that the setting of the positioning block 11 and the group of second fixed rods 8 can drive the first rotating rod 7 to rotate, thereby driving the rotating disk 4 to rotate, and the set first rotating rod 7 drives a group of eccentric blocks 12 to rotate through the connecting unit, so that the T-shaped rod 14 sliding in the T-shaped slide groove 13 and the sliding groove 5 can be driven to rotate. The placement cylinder 6 in the sliding groove 5 cooperates so that the placement cylinder 6 can shake while rotating, thereby improving the mixing efficiency of the culture medium and the cells, allowing the culture medium and the cells to be evenly and fully mixed, so that the cells can fully multiply and grow. The placement cylinder 6 sliding in the sliding groove 5 cannot rotate, and the chain 17 is driven by the first sprocket 15 to drive, thereby driving a group of second sprockets 16 to rotate, so that when the first sprocket 15 rotates, it can also drive a group of second sprockets 16 to rotate, and drive the eccentric block 12 to rotate, thereby driving the placement cylinder 6 to shake, and the culture bottle in the placement cylinder 6 is shaken, so that The culture medium and cells are fully and evenly mixed, and the culture cover 2 is raised and lowered by a group of electric telescopic rods 19, which can greatly improve convenience. When the culture cover 2 is opened, the height of the culture cover 2 can be accurately controlled to support the culture cover 2, thereby facilitating the staff to operate the cultured cells and avoiding damage to the incubator 1 and the culture cover 2 due to excessive opening and closing. A group of ventilation holes 20 and a group of rubber plugs 21 are provided on the culture cover 2, and the gas exchange in the incubator 1 can be controlled to ensure the balance of gas components such as oxygen and carbon dioxide in the culture environment, thereby maintaining the normal physiological function of the cells.
[0030] The above-mentioned front, back, left, right, top and bottom are all based on the figures in the specification. Figure 1 As a benchmark, according to the person's observation perspective, the side of the device facing the observer is defined as the front, the left side of the observer is defined as the left, and so on.
[0031] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the scope of protection of the present invention.
[0032] The above shows and describes the basic principles, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principles of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.
Claims
1. A cell proliferation culture device, characterized in that: The invention comprises an incubator (1), wherein a culture cover (2) is provided on the top of the incubator (1), the incubator (1) is provided with a first groove (3), the bottom of the first groove (3) is rotatably connected to a rotating disk (4) via a first rotating shaft, a group of sliding grooves (5) is provided on the top of the rotating disk (4), a placement cylinder (6) is slidably connected in a group of the sliding grooves (5), a first rotating rod (7) is fixedly connected to the rotating disk (4), a group of second fixed rods (8) are fixedly connected to the first rotating rod (7) via a group of first fixed rods, a power piece is provided on the incubator cover (2), the power piece is used to drive the first rotating rod (7) to rotate, and a shaking assembly is provided on the rotating disk (4), the shaking assembly is used to drive the placement cylinder (6) to slide in the sliding groove (5).
2. A cell proliferation culture device according to claim 1, characterized in that: The power component comprises a motor (9), the motor (9) is fixedly connected to the top of the culture cover (2) via a pair of L-shaped rods, a driving rod (10) is provided at the output end of the motor (9), the bottom of the driving rod (10) is rotatably connected to the top of the culture cover (2), and the bottom of the driving rod (10) passes through the bottom of the culture cover (2), and a locking block (11) is fixedly connected to the bottom of the driving rod (10).
3. A cell proliferation culture device according to claim 2, characterized in that: The shaking assembly comprises a connecting unit and an eccentric block (12); a group of the eccentric blocks (12) are rotatably connected to the rotating disk (4) via a second rotating shaft; a group of the eccentric blocks (12) are provided with a T-shaped groove (13) on the outer circular wall; a group of the T-shaped groove (13) is slidably connected with a T-shaped rod (14); one end of a group of the T-shaped rod (14) is respectively fixed to the outer circular wall of a group of placement cylinders (6).
4. A cell proliferation culture device according to claim 3, characterized in that: The connecting unit comprises a first sprocket (15), the first sprocket (15) is fixedly connected to a first rotating rod (7), a group of eccentric blocks (12) are fixedly connected to the top with a second sprocket (16), and the first sprocket (15) and the second sprocket (16) are connected by a chain (17).
5. A cell proliferation culture device according to claim 4, characterized in that: A group of fixed grooves (18) are provided at the bottom of the incubator (1), and an electric telescopic rod (19) is fixedly connected to the bottom of the fixed grooves (18), and a group of telescopic ends of the electric telescopic rods are fixedly connected to the bottom of the incubator cover (2).
6. A cell proliferation culture device according to claim 5, characterized in that: The culture cover (2) is provided with a group of ventilation holes (20), and the top of the culture cover (2) is fixedly connected with a group of rubber plugs (21).