Electrochemical non-enzyme kit for human body fluid detection

Abstract

The utility model provides an electrochemical non-enzyme kit for human body fluid detection, and relates to the technical field of kits. The electrochemical enzyme-free kit for human body fluid detection comprises a bottle body, a bottle cap, two self-suction type connecting columns and a bottle bottom containing a sensor, one end of the bottle body is connected with the bottle cap, and the other end of the bottle body is connected with the bottle bottom; an enzyme-free three-electrode solid sensor is embedded in the surface, close to the bottle body, of the bottle bottom and used for detecting the biological index concentration of a human body fluid sample, and a wire interface is formed in the surface, away from the bottle body, of the bottle bottom; the two self-suction type connecting columns penetrate through the bottle bottom to be embedded in the bottle body. The electrochemical enzyme-free kit for human body fluid detection provided by the utility model solves the technical problems that in the prior art, electrode cross contamination is caused, and a household blood sugar or uric acid detector is inconvenient to operate and causes minimally invasive injury.

Description

Technical Field

[0001] The utility model relates to the technical field of test kits, in particular to an electrochemical enzyme-free test kit for detecting human body fluids. Background Art

[0002] A home blood glucose meter is an instrument for measuring the blood glucose concentration in the blood, and a home uric acid tester is an instrument for measuring the uric acid concentration. Existing home blood glucose meters and home uric acid testers are mostly provided with a connector for inserting a test strip on one side of the tester body. Before testing blood glucose or uric acid parameters, it is necessary to first collect blood with the help of a blood collection needle and then drop the collected blood onto the test strip. During the operation, it is necessary to draw blood samples from the patient's fingertips or other parts, which brings risks of minimally invasive, pain and infection to the user. Utility Model Content

[0003] The utility model aims to provide an electrochemical enzyme-free kit for human body fluid detection, so as to solve the technical problems of electrode cross contamination and inconvenient operation of household blood glucose or uric acid detectors in the prior art, and cause minimally invasive surgery.

[0004] In order to solve the above technical problems, the technical solution provided by the utility model is:

[0005] The utility model provides an electrochemical enzyme-free test kit for detecting human body fluids, comprising a bottle body, a bottle cap, two self-priming connection columns and a bottle bottom containing a sensor;

[0006] One end of the bottle body is connected to the bottle cap, and the other end is connected to the bottle bottom, so that the sample injection pen can be inserted from the bottle cap and inject the human body fluid sample;

[0007] A non-enzyme three-electrode solid sensor is embedded on the surface of the bottle bottom close to the bottle body, and the non-enzyme three-electrode solid sensor is used to detect the concentration of biological indicators of the human body fluid sample, and a wire interface is provided on the surface away from the bottle body;

[0008] The two self-priming connecting columns pass through the bottle bottom and are embedded in the bottle body.

[0009] Furthermore, the enzyme-free three-electrode solid sensor comprises a counter electrode, a working electrode and a reference electrode;

[0010] The counter electrode, the working electrode and the reference electrode are arranged at intervals.

[0011] Furthermore, the working electrode material is conductive mixed carbon paste; the counter electrode material is conductive carbon paste; and the reference electrode material is conductive silver paste.

[0012] Furthermore, both of the two self-priming connecting columns are magnetic.

[0013] Furthermore, the inner wall of the bottle body is provided with a mounting protrusion, and the lower surface of the mounting protrusion is provided with two mounting grooves;

[0014] The bottle bottom is bonded to the lower surface of the mounting protrusion, and the two self-priming connecting columns are respectively inserted into the two mounting grooves.

[0015] Furthermore, the two installation grooves are symmetrically arranged.

[0016] Furthermore, the bottle body is provided with a first axial positioning structure, and the bottle bottom is provided with a second axial positioning structure, and the first axial positioning structure matches the second axial positioning structure.

[0017] Furthermore, the bottle cap is threadably connected to the bottle body.

[0018] Furthermore, the bottle cap includes an operating portion and a connecting portion, the operating portion is connected to the connecting portion, and the connecting portion is threadedly connected to the bottle body;

[0019] The outer wall of the operating part is provided with an anti-slip structure.

[0020] Furthermore, a centering structure is installed in the bottle body.

[0021] Based on the above technical problems, the technical effects that can be achieved by the utility model are analyzed as follows:

[0022] The utility model provides an electrochemical enzyme-free test kit for detecting human body fluids, comprising a bottle body, a bottle cap, two self-priming connecting columns and a bottle bottom containing a sensor; one end of the bottle body is connected to the bottle cap, and the other end is connected to the bottle bottom, so that a sample injection pen can be inserted from the bottle cap and injected into a human body fluid sample; a non-enzyme three-electrode solid sensor is embedded on the surface of the bottle bottom close to the bottle body, and the non-enzyme three-electrode solid sensor is used to detect the concentration of biological indicators of human body fluid samples, and a wire interface is provided on the surface away from the bottle body; the two self-priming connecting columns pass through the bottle bottom and are embedded in the bottle body.

[0023] The electrochemical enzyme-free kit for human body fluid detection is used in conjunction with a sample injection pen and an electrochemical detector. When in use, the electrochemical enzyme-free kit for human body fluid detection is installed on the electrochemical detector through two self-priming connection columns, and the human body fluid sample is injected into the bottle of the electrochemical enzyme-free kit for human body fluid detection by using the sample injection pen; the biological index concentration of the human body fluid sample, such as blood sugar, uric acid and vitamins, undergoes oxidation-reduction reaction on the enzyme-free three-electrode solid sensor to generate an electrical signal; the electrochemical detector can convert the electrical signal into the content of the corresponding biological index.

[0024] The electrochemical enzyme-free kit for human body fluid detection is a disposable, portable and pre-packaged kit. It uses an enzyme-free three-electrode solid sensor to detect biological indicators such as blood sugar, uric acid or vitamins. It is easy to use, simple to operate and avoids cross contamination. It achieves high sensitivity and convenience while avoiding the complex enzyme reaction steps in traditional methods, overcoming the shortcomings of microneedle traumatic blood sampling detection methods. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 A schematic diagram of the three-dimensional structure of an electrochemical enzyme-free kit for human body fluid detection provided by an embodiment of the utility model;

[0027] Figure 2 A cross-sectional view of an electrochemical enzyme-free kit for detecting human body fluids provided in an embodiment of the utility model;

[0028] Figure 3 Schematic diagram of the structure of the bottle bottom in the electrochemical enzyme-free kit for human fluid detection provided by the embodiment of the utility model Figure 1 ;

[0029] Figure 4 Schematic diagram of the structure of the bottle bottom in the electrochemical enzyme-free kit for human fluid detection provided by the embodiment of the utility model Figure 2 ;

[0030] Figure 5 A schematic diagram of the structure of the bottle in the electrochemical enzyme-free kit for human fluid detection provided by an embodiment of the utility model;

[0031] Figure 6 A schematic diagram of the structure of a bottle cap in an electrochemical enzyme-free kit for human fluid detection provided by an embodiment of the utility model;

[0032] Figure 7 This is a schematic diagram of an electrochemical enzyme-free kit for human fluid detection provided by an embodiment of the utility model being installed in an electrochemical detector.

[0033] icon:

[0034] 100-bottle body; 110-mounting protrusion; 111-mounting groove; 120-first axial positioning structure;

[0035] 200-bottle cap; 210-operating part; 211-anti-slip structure; 220-connecting part;

[0036] 300-bottom of bottle; 310-enzyme-free three-electrode solid sensor; 311-counter electrode; 312-working electrode; 313-reference electrode; 320-wire interface; 330-second axial positioning structure;

[0037] 400-Self-priming connecting column;

[0038] 500-Centering structural parts. DETAILED DESCRIPTION

[0039] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0040] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0042] In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate the orientation or position relationship based on the orientation or position relationship shown in the accompanying drawings, or the orientation or position relationship in which the utility model product is usually placed when in use, which is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0043] In addition, the terms "horizontal", "vertical", "overhanging" and the like do not mean that the components are required to be absolutely horizontal or overhanging, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0044] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0045] In conjunction with the accompanying drawings, some embodiments of the present invention are described in detail below. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0046] Existing blood glucose meters or uric acid testers have problems such as inconvenient operation, minimally invasive blood sampling and poor accuracy.

[0047] In view of this, see Figures 1 to 7 The electrochemical enzyme-free kit for human body fluid detection provided by the embodiment of the utility model includes a bottle body 100, a bottle cap 200, two self-priming connecting columns 400 and a bottle bottom 300 containing a sensor; one end of the bottle body 100 is connected to the bottle cap 200, and the other end is connected to the bottle bottom 300, so as to enable the sample spray pen to be inserted from the bottle cap 200 and inject the human body fluid sample; an enzyme-free three-electrode solid sensor 310 is embedded on the surface of the bottle bottom 300 close to the bottle body 100, and the enzyme-free three-electrode solid sensor 310 is used to detect the concentration of biological indicators of human body fluid samples, and a wire interface 320 is provided on the surface away from the bottle body 100; two self-priming connecting columns 400 pass through the bottle bottom 300 and are embedded in the bottle body 100.

[0048] Specifically, the electrochemical enzyme-free kit for human body fluid detection can detect blood sugar concentration alone, detect uric acid concentration alone, or detect blood sugar concentration and uric acid concentration at the same time; of course, the kit can also detect the concentration of other biological indicators. Furthermore, the bottle bottom 300 is set as a circuit board substrate with printed electrodes, and of course the bottle bottom 300 is not limited to the circuit board substrate; the circuit board is provided with an enzyme-free three-electrode solid sensor 310, and the wire interface 320 at the bottom of the bottle bottom 300 is used to connect to the electrochemical detector and output signals to the electrochemical detector. In this embodiment, the thickness of the bottle bottom 300 is set to 1.6mm. Preferably, the bottle body 100 is made of medical PP material.

[0049] The electrochemical enzyme-free kit for human body fluid detection is used in conjunction with a sample injection pen and an electrochemical detector. When in use, the electrochemical enzyme-free kit for human body fluid detection is installed on the electrochemical detector through two self-priming connecting columns 400, and the human body fluid sample is injected into the bottle body 100 of the electrochemical enzyme-free kit for human body fluid detection by using the sample injection pen; the biological index concentration of the human body fluid sample, such as blood sugar, uric acid and vitamins, undergoes an oxidation-reduction reaction on the enzyme-free three-electrode solid sensor to generate an electrical signal; the electrochemical detector can convert the electrical signal into the content of the corresponding biological index.

[0050] The following uses saliva to detect blood sugar and uric acid as an example. A certain amount of saliva is mixed with a certain amount of homemade sample diluent. The electrochemical detector uses the electrochemical chronoamperometry to apply a potential to the solution, so that the blood sugar and uric acid in the saliva undergo an oxidation-reduction reaction on the enzyme-free three-electrode solid sensor 310 to generate an electrical signal; the electrochemical detector collects the electrical signal and converts the current into a potential through an I / V conversion circuit, and then filters the potential through a low-pass filter circuit to remove interference signals. The tiny potential signal is amplified through an amplifier circuit to improve the detection accuracy, and finally the processed signal is converted from the analog potential signal to a digital signal through an A / D converter to be read by the electrochemical detector. The electrochemical detector compares the electrical signal values ​​of standard samples of blood sugar and uric acid with different concentrations to develop a standard curve, and obtains the content of blood sugar and uric acid in the sample to be tested based on the electrical signal value generated by the sample to be tested.

[0051] The electrochemical enzyme-free kit for human body fluid detection is a disposable, portable and pre-packaged kit. It uses an enzyme-free three-electrode solid sensor 310 to detect biological indicators such as blood sugar, uric acid or vitamins. It is easy to use, simple to operate and avoids cross contamination. It achieves high sensitivity and convenience while avoiding the complex enzyme reaction steps in traditional methods, overcoming the shortcomings of microneedle blood sampling trauma detection methods.

[0052] The following is a detailed description of the structure and shape of the electrochemical enzyme-free kit for human body fluid detection:

[0053] In the optional scheme of the embodiment of the utility model, please see Figure 3 The enzyme-free three-electrode solid sensor 310 includes a counter electrode 311, a working electrode 312 and a reference electrode 313; the counter electrode 311, the working electrode 312 and the reference electrode 313 are arranged at intervals.

[0054] Specifically, nanoparticles of suitable size and surface modification are first prepared, and then the nanoparticles are combined with an electrochemical sensor to form an enzyme-free three-electrode solid sensor 310 capable of measuring blood sugar or uric acid. Furthermore, the nanoparticles can be selected from metal nanoparticles (Au, Ag, Pt nanoparticles), carbon-based nanomaterials (graphene, carbon nanotubes, carbon dots, fullerenes), metal oxide semiconductor materials (black TiO2, Fe3O4), metal sulfides (MoS2), etc.; the modification of the nanoparticles can be selected from: amino acid-mediated surface modification, thiol-mediated surface modification, ligand exchange-based surface modification, etc.; or, MXene materials are used to replace nanoparticles, and MXene materials include Ti3C2, Ti2C, Nb2C, V2C, Mo2C, etc.; or, magnetic particles or magnetic beads are used, such as silica gel-coated magnetic beads. Of course, other materials and modification types should also be within the scope of protection of the embodiments of the utility model as long as the size meets the requirements and the requirements of excellent electrical properties are met. In this embodiment, the material of the working electrode 312 is a conductive mixed carbon paste; the material of the counter electrode 311 is a conductive carbon paste; and the material of the reference electrode 313 is a conductive silver paste. Figure 3 , the counter electrode 311 is configured to be arc-shaped, the working electrode 312 and the reference electrode 313 are both configured to be circular, and the diameter of the working electrode 312 is greater than the diameter of the reference electrode 313; of course, the shapes and arrangements of the counter electrode 311, the working electrode 312 and the reference electrode 313 are not limited to Figure 3 Other shapes and arrangements should also be within the protection scope of the embodiments of the present utility model.

[0055] The counter electrode 311 , the working electrode 312 and the reference electrode 313 realize the redox effect of the enzyme-free three-electrode solid sensor 310 on the saliva sample.

[0056] In an optional solution of the embodiment of the utility model, both of the two self-priming connecting columns 400 are magnetic.

[0057] Specifically, see Figure 2 , the two self-priming connecting columns 400 are both configured as cylinders with equal diameters. In this embodiment, the self-priming connecting columns 400 are configured as magnetic iron columns.

[0058] The self-priming connection column 400 is magnetic and is used to magnetically cooperate with the magnetic contact base on the electrochemical detector, so that the bottom of the electrochemical enzyme-free kit for human fluid detection can be stably installed on the electrochemical detector.

[0059] In an optional scheme of the embodiment of the utility model, the inner wall of the bottle body 100 is provided with a mounting protrusion 110, and the lower surface of the mounting protrusion 110 is provided with two mounting grooves 111; the bottle bottom 300 is bonded to the lower surface of the mounting protrusion 110, and the two self-priming connecting columns 400 are respectively inserted into the two mounting grooves 111.

[0060] Specifically, see Figure 5 The mounting protrusion 110 protrudes toward the inside of the bottle body 100 .

[0061] The bottle bottom 300 is bonded to the lower surface of the mounting protrusion 110, which increases the contact area between the bottle bottom 300 and the bottle body 100, enhances the connection strength between the two, and prevents the bottle bottom 300 from falling off the bottle body 100. The two self-priming connecting columns 400 are respectively inserted into the two mounting grooves 111 to achieve the connection between the self-priming connecting columns 400 and the bottle body 100.

[0062] In an optional solution of the embodiment of the utility model, the bottle body 100 is provided with a first axial positioning structure 120, and the bottle bottom 300 is also provided with a second axial positioning structure 330. The first axial positioning structure 120 cooperates with the second axial positioning structure 330 to form axial positioning for the bottle body 100, thereby avoiding the wrong installation direction of the bottle bottom 300 and improving the installation efficiency.

[0063] In an optional solution of the embodiment of the utility model, the two installation grooves 111 are symmetrically arranged.

[0064] Specifically, the symmetry lines of the two mounting grooves 111 are perpendicular to the first positioning plane.

[0065] The two mounting grooves 111 are symmetrically arranged to realize the symmetrical arrangement of the two self-priming connecting columns 400, which helps to stably install the bottom of the electrochemical enzyme-free reagent kit for human fluid detection on the electrochemical detector, ensuring that the electrode at the bottle bottom 300 is connected to the electrode needle of the electrochemical detector.

[0066] In an optional solution of the embodiment of the utility model, the bottle cap 200 is threadedly connected to the bottle body 100 .

[0067] Specifically, the bottle cap 200 is provided with an external thread, and the inner wall of the top of the bottle body 100 is provided with an internal thread, and the external thread of the bottle cap 200 is threadedly connected with the internal thread of the bottle body 100 .

[0068] The bottle cap 200 is threadably connected to the bottle body 100 , making it easy to install and remove the bottle cap 200 .

[0069] In the optional scheme of the embodiment of the utility model, please see Figure 6The bottle cap 200 includes an operating portion 210 and a connecting portion 220 , the operating portion 210 is connected to the connecting portion 220 , and the connecting portion 220 is threadedly connected to the bottle body 100 ; an anti-slip structure 211 is provided on the outer wall of the operating portion 210 .

[0070] Specifically, the anti-slip structure 211 includes a plurality of grooves spaced apart along the circumference of the operating portion 210, and each groove extends along the axial direction of the operating portion 210. Furthermore, a sealing rubber pad is provided in the middle of the operating portion 210, and the bottle cap 200 is made of PVC material. A sealing ring is provided on the outer periphery of the connecting portion 220 to prevent liquid from leaking from the bottle cap 200.

[0071] The anti-slip structure 211 helps to increase the friction between the bottle cap 200 and the operator's hand when installing or removing the bottle cap 200, thereby facilitating unscrewing or tightening the bottle cap 200.

[0072] In the optional scheme of the embodiment of the utility model, please see Figure 2 A centering structure 500 is installed in the bottle body 100 .

[0073] Specifically, the centering structure 500 is fixed at the middle position of the bottle body 100, and the axis of the centering structure 500 is colinear with the axis of the bottle body 100. Preferably, the centering structure 500 is made of medical PP material to ensure that the head of the spray pen is always aligned with the middle of the bottle bottom.

[0074] The centering structure 500 is used to ensure that the sample spray pen can be accurately aligned with the center after being inserted into the bottle body 100.

[0075] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solution deviate from the scope of the technical solution of the embodiments of the utility model.

Claims

1. An electrochemical enzyme-free kit for human body fluid detection, characterized in that: include: A bottle body (100), a bottle cap (200), two self-priming connecting columns (400) and a bottle bottom (300) containing a sensor; One end of the bottle body (100) is connected to the bottle cap (200), and the other end is connected to the bottle bottom (300), so as to allow the sample injection pen to be inserted from the bottle cap (200) and to inject the human body fluid sample; A non-enzyme three-electrode solid sensor (310) is embedded on the surface of the bottle bottom (300) close to the bottle body (100), and the non-enzyme three-electrode solid sensor (310) is used to detect the concentration of biological indicators of the human body fluid sample, and a wire interface (320) is provided on the surface away from the bottle body (100); The two self-priming connecting columns (400) pass through the bottle bottom (300) and are embedded in the bottle body (100).

2. The electrochemical enzyme-free kit for human body fluid detection according to claim 1, characterized in that: The enzyme-free three-electrode solid sensor (310) comprises a counter electrode (311), a working electrode (312) and a reference electrode (313); The counter electrode (311), the working electrode (312) and the reference electrode (313) are arranged at intervals.

3. The electrochemical enzyme-free kit for human body fluid detection according to claim 2, characterized in that: The material of the working electrode (312) is conductive mixed carbon paste; the material of the counter electrode (311) is conductive carbon paste; and the material of the reference electrode (313) is conductive silver paste.

4. The electrochemical enzyme-free kit for human body fluid detection according to claim 1, characterized in that: The two self-priming connecting columns (400) are both magnetic.

5. The electrochemical enzyme-free kit for human body fluid detection according to claim 1, characterized in that: The inner wall of the bottle body (100) is provided with a mounting protrusion (110), and the lower surface of the mounting protrusion (110) is provided with two mounting grooves (111); The bottle bottom (300) is bonded to the lower surface of the mounting protrusion (110), and the two self-priming connecting columns (400) are respectively inserted into the two mounting grooves (111).

6. The electrochemical enzyme-free kit for human body fluid detection according to claim 5, characterized in that: The two installation grooves (111) are symmetrically arranged.

7. The electrochemical enzyme-free kit for human body fluid detection according to claim 1, characterized in that: The bottle body (100) is provided with a first axial positioning structure (120), and the bottle bottom (300) is provided with a second axial positioning structure (330), and the first axial positioning structure (120) cooperates with the second axial positioning structure (330).

8. The electrochemical enzyme-free kit for human body fluid detection according to claim 1, characterized in that: The bottle cap (200) is threadably connected to the bottle body (100).

9. The electrochemical enzyme-free kit for human body fluid detection according to claim 8, characterized in that: The bottle cap (200) comprises an operating portion (210) and a connecting portion (220), wherein the operating portion (210) is connected to the connecting portion (220), and the connecting portion (220) is threadedly connected to the bottle body (100); The outer wall of the operating portion (210) is provided with an anti-slip structure (211).

10. The electrochemical enzyme-free kit for human body fluid detection according to claim 1, characterized in that: A centering structure (500) is installed in the bottle body (100).

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