Portable immunoblotting analyzer

Abstract

The utility model provides a portable immunoblotting analyzer which comprises a machine case, a machine case and a bearing table, and further comprises a cleaning device which is arranged in a machine cavity and comprises a base table with a plurality of upper opening grooves and a cleaning mechanism with a time limiting function, and a clamping device which is arranged in the machine cavity and is used for clamping the base table and the cleaning mechanism. The clamping device is used for automatically clamping and moving the solid-phase carrier membrane placed on the bearing table into the corresponding open slot; a film detection device having an imaging function; the control device comprises a circuit board and a cleaning triggering piece electrically connected with the circuit board; and after the membrane detection device detects that the cleanliness of the solid-phase carrier reaches the standard, the clamping device is started to move the solid-phase carrier away from the base station. Compared with the prior art, the cleaning device and the film detection device with the shooting function are arranged, so that the trouble of waste of effort and effort caused by frequent and repeated cleaning is eliminated, and the automatic cleaning device has the advantages of automatic cleaning function, high cleaning efficiency and convenience in cleaning operation; and popularization and application of the product are facilitated.

Description

Technical Field

[0001] The utility model belongs to the technical field of instrument analysis equipment, in particular to a portable immunoblotting analyzer. Background Art

[0002] Immunoblotting, also known as Western blotting, is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. This method is a new immunobiochemical technique developed on the basis of gel electrophoresis and solid phase immunoassay. Since immunoblotting has the high resolution of SDS-PAGE and the high specificity and sensitivity of solid phase immunoassay, it has become a routine technique for protein analysis. Immunoblotting is often used to identify a certain protein, and can perform qualitative and semi-quantitative analysis of the protein. Combined with chemiluminescence detection, it can simultaneously compare the expression differences of the same protein in multiple samples. Immunoblotting detects through specific immune reactions between antibodies and antigenic epitopes presented by target proteins attached to solid phase supports. The whole process includes: protein separation, electrotransfer, immunological detection and signal detection.

[0003] In the prior art, for example, the utility model patent authorized by China is publicly named as a compact immunoblot analyzer, and the patent publication number is CN218974378U, and specifically discloses "a compact immunoblot analyzer, which includes: a shielded chassis; a main control circuit board fixedly arranged on the inner bottom plate of the shielded chassis; a linear module horizontally arranged above the main control circuit board, and a movable end of which is fixedly connected to a horizontally arranged mold strip loading plate; a camera mechanism fixedly arranged above the mold strip loading plate, and a fill light fixedly arranged on the peripheral wall of the camera mechanism; and two control buttons arranged on the shielded chassis". Although the analyzer of this structure is a compact device, the device does not involve the cleaning function of the solid phase carrier membrane; in the experiment, the solid phase carrier membrane is cleaned manually for many times and frequently, and it is judged whether it is clean by feeling, so there are technical problems such as no automatic cleaning function, low cleaning efficiency and inconvenient cleaning operation.

[0004] To this end, the utility model provides a convenient immunoblotting analyzer. Utility Model Content

[0005] In view of the above problems existing in the prior art, the purpose of the utility model is to provide a portable immunoblot analyzer having the advantages of automatic cleaning function, high cleaning efficiency and convenient cleaning operation.

[0006] The purpose of the utility model can be achieved through the following technical solutions:

[0007] A portable immunoblotting analyzer comprises a chassis having a machine cavity;

[0008] A carrier platform, which is used to place a plurality of solid phase carrier membranes made of proteins;

[0009] Also included is a cleaning device, which is arranged in the machine cavity, and includes a base with a plurality of upper opening slots and a cleaning mechanism with a time-limited function, and the cleaning mechanism is located on the side of the base;

[0010] A clamping device, which is arranged in the machine cavity and is used to automatically clamp and move the solid phase carrier film placed on the carrier platform to the corresponding upper opening groove;

[0011] A membrane detection device with a photographing function, which is arranged in the machine cavity and directly opposite to the upper opening groove, and is used to detect the cleanliness of the surface of the solid phase carrier membrane;

[0012] A control device comprises a circuit board and a cleaning trigger electrically connected to the circuit board, wherein the cleaning device, the clamping device and the membrane detection device are electrically connected to the circuit board respectively; after the cleaning trigger is triggered, the cleaning mechanism performs time-limited cleaning on the solid phase carrier membrane placed in the upper opening groove; after the membrane detection device detects that the cleanliness of the solid phase carrier meets the standard, the clamping device is activated to move the solid phase carrier away from the base.

[0013] As a preferred technical solution of the utility model, the cleaning mechanism includes:

[0014] A liquid tank containing cleaning liquid and having a water supply port and a water outlet;

[0015] A liquid pump; the liquid pump is built into the liquid tank, and a signal end of the liquid pump is electrically connected to the circuit board;

[0016] A liquid pipe, the water inlet end of which extends into the water outlet and communicates with the water through hole of the liquid pump, the liquid pipe is upwardly directed, hidden in the top of the chassis and arranged downwardly along the longitudinal direction;

[0017] A spray head having a plurality of spray holes, the top end of the spray head being connected to the water outlet end of the liquid pipe, and the spray holes and the upper opening groove being arranged opposite to each other;

[0018] A timer is integrated in the circuit board, and a signal end of the timer is electrically connected to a signal end of the liquid pump.

[0019] As a preferred technical solution of the utility model, it also includes a waste liquid purification mechanism, which has:

[0020] A waste liquid collection chamber, which is arranged on the base, and the waste liquid collection chamber is connected with each of the upper opening grooves;

[0021] A waste liquid purification box, one opening of which is connected to the waste liquid collection chamber through a pipeline, and another opening of which is connected to the liquid tank through another pipeline;

[0022] A liquid purifier is embedded in the waste liquid purification box, and is used to purify the liquid that flows back into the waste liquid purification box.

[0023] As a preferred technical solution of the utility model, the liquid purifier is a liquid purification filter with a composite filter element and an RO reverse osmosis membrane, and its model is MR1253-A.

[0024] As a preferred technical solution of the present utility model, the liquid contained in the liquid tank is pure water.

[0025] As a preferred technical solution of the utility model, the cleaning mechanism includes a plasma cleaning structure, which has:

[0026] A cleaning box with an air inlet and an air outlet, a positive electrode plate, a negative electrode plate and a vacuum pump, wherein the cleaning box can be turned over to cover the outer peripheral edge of the base, the positive electrode plate is embedded in the left side of the inner cavity of the cleaning box along the longitudinal direction, and the negative electrode plate is embedded in the right side of the inner cavity of the cleaning box along the longitudinal direction;

[0027] During cleaning, the air inlet is closed, the air suction end of the vacuum pump is connected to the air outlet of the cleaning box, and the positive electrode plate, the negative electrode plate and the power transmission end of the vacuum pump are electrically connected to the circuit board through wires.

[0028] As a preferred technical solution of the utility model, the cleaning mechanism further includes a shielding layer.

[0029] The left and right ends of the shielding layer are in contact between the top of the positive electrode plate and the top of the negative electrode plate, and the shielding layer is attached to the top surface of the inner cavity of the cleaning box.

[0030] As a preferred technical solution of the utility model, the clamping device includes a bending seat embedded in the inner wall of the machine cavity, a rotating motor and a clamping hand, the base of the rotating motor is fixed on the horizontal plate of the bending seat, the power transmission end of the rotating motor is electrically connected to the circuit board, and the clamping hand is fixed to the shaft of the rotating motor.

[0031] As a preferred technical solution of the utility model, the film detection device includes a support platform and a shooting camera, and the base of the shooting camera is fixed to the support platform;

[0032] The shooting position of the shooting camera is arranged opposite to the solid phase carrier film placed in the upper opening groove, and the signal end of the shooting camera is electrically connected to the circuit board.

[0033] As a preferred technical solution of the utility model, it also includes a reagent preservation device, which includes a coating block coated with a reagent obtained after an immunoassay using a solid phase carrier membrane, a cooling plate and a temperature sensor, the cooling plate is attached to the surface of the coating block, the temperature sensor is used to detect the temperature of the coating block, and the temperature sensor is electrically connected to the circuit board.

[0034] As described above, the portable immunoblot analyzer provided by the utility model has the following beneficial effects:

[0035] The utility model utilizes the above-mentioned portable immunoblot analyzer. Compared with the prior art, due to the adoption of such a structure, a cleaning device and a membrane detection device with a shooting function are provided. Compared with the existing structure adopting manual cleaning, the cleaning mechanism with a time-limited function can be used to perform time-limited cleaning on the solid phase carrier membrane, and then the cleanliness is detected in combination with the membrane detection device. Only after the cleanliness meets the standard will the solid phase carrier be moved away from the base through the clamping device, thereby eliminating the trouble and labor caused by frequent and repeated cleaning. The utility model has the advantages of automatic cleaning function, high cleaning efficiency and convenient cleaning operation; and it is conducive to the promotion and application of the product. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 A three-dimensional diagram of the portable immunoblotting analyzer applied for in the utility model;

[0037] Figure 2 This is a schematic diagram of the internal structure of the portable immunoblot analyzer (excluding the clamping device) of the utility model application from another perspective, and the cleaning mechanism is the structure of Example 1;

[0038] Figure 3 for Figure 1 Schematic diagram of the internal structure from another perspective;

[0039] Figure 4 This is a block diagram of the operation control structure of the portable immunoblotting analyzer applied for by the utility model;

[0040] Figure 5 The washing mechanism in the portable immunoblot analyzer applied for the utility model is a structural diagram of Example 2;

[0041] Figure 6 This is a schematic diagram of the structure of the clamping device in the portable immunoblotting analyzer applied for by the utility model;

[0042] Figure 7 The present invention is a schematic diagram of the structure of a reagent storage device in a portable immunoblotting analyzer. DETAILED DESCRIPTION

[0043] The following is a description of the implementation of the present invention by means of specific embodiments. People familiar with the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0044] It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions that can be implemented in this utility model, so they have no substantial technical significance. Any modification of the structure, change of the proportional relationship or adjustment of the size, without affecting the effects that can be produced by the utility model and the purposes that can be achieved, should still fall within the scope of the technical content disclosed in this utility model. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of this utility model. The change or adjustment of their relative relationship, without substantial change in the technical content, should also be regarded as the scope of the implementation of this utility model. The specific structure can be described with reference to the drawings of the patent application.

[0045] The utility model provides a convenient immunoblotting analyzer. Figures 1 to 7 As shown, including:

[0046] A chassis 1 having a machine cavity 10;

[0047] A carrier platform 2, which is used to place a plurality of solid phase carrier membranes 3 made of proteins;

[0048] It also includes a cleaning device 4, which is arranged in the machine cavity 10, and includes a base 40 with a plurality of upper opening slots 400 and a cleaning mechanism 41 with a time-limited function, and the cleaning mechanism 41 is located on the side of the base 40;

[0049] A clamping device 5, which is disposed in the machine cavity 10 and is used to automatically clamp and move the solid phase carrier film 3 placed on the carrier platform 2 to the corresponding upper opening groove 400;

[0050] A film detection device 6 with a photographing function, which is disposed in the machine cavity 10 and directly opposite to the upper opening groove 400, and is used to detect the cleanliness of the surface of the solid phase carrier film 3;

[0051] The control device 7 includes a circuit board 70 and a cleaning trigger 71 electrically connected to the circuit board 70, and the cleaning device 41, the clamping device 5 and the membrane detection device 6 are electrically connected to the circuit board 70 respectively; after the cleaning trigger 71 is triggered, the cleaning mechanism 41 performs a time-limited cleaning on the solid phase carrier membrane 3 placed in the upper opening groove 400; after the membrane detection device 6 detects that the cleanliness of the solid phase carrier membrane 3 meets the standard, the clamping device 5 is started to move the solid phase carrier membrane 3 away from the base 40.

[0052] When in use, the solid phase carrier film 3 is first placed on the supporting platform 2, and then the clamping device 5 is started, and the solid phase carrier film 3 is clamped into the upper opening groove 400 at the base 40 by the clamping device 5, and then the cleaning trigger 71 is started, and then the circuit board 70 of the control device 7 starts the cleaning mechanism 41 of the cleaning device 4 to perform time-limited cleaning on the solid phase carrier film 3 placed in the upper opening groove 400; after the membrane detection device 6 detects that the cleanliness of the solid phase carrier film 3 meets the standard, the clamping device 5 is started to move the solid phase carrier film 3 away from the base 40; the entire cleaning process can be completed within a limited time, thereby eliminating the trouble and labor caused by frequent and repeated cleaning, and has the advantages of automatic cleaning function, high cleaning efficiency, and convenient cleaning operation; and it is conducive to the promotion and application of the product.

[0053] For the above-mentioned cleaning mechanism 41, the present invention provides two specific structures for illustration, but is not limited thereto. Specific embodiment 1 of the cleaning mechanism 41 is shown below.

[0054] Combination Figure 1 , Figure 2 as well as Figure 4 As shown, in this embodiment, the cleaning mechanism 41 includes:

[0055] A liquid tank 410 containing cleaning liquid and having a water supply port and a water outlet;

[0056] A liquid pump 411 is built into the liquid tank 410 , and a signal end of the liquid pump 411 is electrically connected to the circuit board 70 ;

[0057] A liquid pipe 412, whose water inlet end extends into the water outlet and is connected to the water through hole of the liquid pump 411, and the liquid pipe 412 is upwardly directed, hidden in the top of the chassis 1 and arranged downwardly along the longitudinal direction;

[0058] The nozzle 413 has a plurality of spray holes (not shown in the figure, and the same applies to the following), the top of the nozzle 413 is connected to the water outlet end of the liquid pipe 412, and the spray holes and the upper opening groove 400 are arranged opposite to each other;

[0059] A time limiter 414 is integrated in the circuit board, and a signal end of the time limiter 414 is electrically connected to a signal end of the liquid pump 411 .

[0060] In order to treat the waste liquid after cleaning, Figure 2 and Figure 3 As shown, in this embodiment, a waste liquid purification mechanism 8 is also included, which has:

[0061] A waste liquid collection chamber 80 is disposed on the base 40, and the waste liquid collection chamber 80 is connected to each of the upper opening grooves 400;

[0062] A waste liquid purification box 81, one opening of which is connected to the waste liquid collection chamber 80 through a pipeline, and another opening of which is connected to the liquid box 81 through another pipeline;

[0063] The liquid purifier 82 is embedded in the waste liquid purification box 81, and is used to purify the liquid that flows back into the waste liquid purification box 81. The waste liquid purification mechanism 8 is provided to recycle and purify the waste liquid after cleaning, so as to avoid direct discharge into the external environment and polluting the environment, and has the technical effect of recycling and purifying the waste liquid and protecting the environment.

[0064] In this embodiment, the liquid purifier 82 is a liquid purifier filter having a composite filter element and a RO reverse osmosis membrane, and its model is MR1253-A.

[0065] In this embodiment, the liquid contained in the liquid tank 81 is pure water.

[0066] The specific embodiment 2 of the cleaning mechanism is described in detail below.

[0067] In this embodiment, combined with Figure 1 , Figure 2 as well as Figure 5 As shown, the cleaning mechanism 41 includes a plasma cleaning structure, which has:

[0068] A cleaning box 415 having an air inlet and an air outlet, a positive electrode plate 416, a negative electrode plate 417 and a vacuum pump 418, wherein the cleaning box 415 can be flipped over to cover the outer peripheral edge of the base 40, the positive electrode plate 416 is embedded in the left side of the inner cavity of the cleaning box 415 along the longitudinal direction, and the negative electrode plate 417 is embedded in the right side of the inner cavity of the cleaning box 415 along the longitudinal direction;

[0069] During cleaning, the air inlet is closed, the air suction end of the vacuum pump 418 is connected to the air outlet of the cleaning box 415, and the positive electrode plate 416, the negative electrode plate 417 and the power transmission end of the vacuum pump 418 are electrically connected to the circuit board 70 through wires. During cleaning, the plasma cleaning structure is first powered on to start the vacuum pump 418 to vacuum the air inside the cleaning box 415 after the base is covered. After completion, positively charged electrons collide with each other under the action of the electric field to form plasma, and the huge kinetic energy generated by the ionization is used to destroy the pollutants on the solid phase carrier and clean it.

[0070] It should be noted that the specific cleaning method of this embodiment is different from that of the specific embodiment 1 of the cleaning mechanism. This embodiment 2 adopts a plasma cleaning method.

[0071] As shown in the figure, in this embodiment, Figure 5 As shown, the cleaning mechanism 41 further includes a shielding layer 419.

[0072] The left and right ends of the shielding layer 419 abut between the top of the positive electrode plate 416 and the top of the negative electrode plate 417 , and the shielding layer 419 is attached to the top surface of the inner cavity of the cleaning box 415 .

[0073] Combination Figure 3 and Figure 6 As shown, in this embodiment, the clamping device 5 includes a bending seat 50 embedded in the inner wall of the machine cavity 10, a rotating motor 51 and a clamping hand 52, the base of the rotating motor 51 is fixed on the horizontal plate of the bending seat 50, the power transmission end of the rotating motor 51 is electrically connected to the circuit board 70, and the clamping hand 52 is fixed to the shaft of the rotating motor 51.

[0074] Combination Figure 2 and Figure 4 As shown, in this embodiment, the film detection device 6 includes a support platform 60 and a camera 61, and the base of the camera 61 is fixed to the support platform 60;

[0075] The shooting position of the shooting camera 61 is arranged opposite to the solid phase carrier film 3 placed in the upper opening groove 400 , and the signal end of the shooting camera 61 is electrically connected to the circuit board 70 .

[0076] Combination Figure 2 and Figure 7 As shown, in this embodiment, in order to preserve the treated reagent, a reagent preservation device 9 is also included, and the reagent preservation device 9 includes a coating block 90 coated with the reagent obtained after the immunoassay with the solid phase carrier membrane 3, a cooling sheet 91 and a temperature sensor 92, the cooling sheet 91 is attached to the surface of the coating block 90, the temperature sensor 92 is used to detect the temperature of the coating block 90, and the temperature sensor 92 is electrically connected to the circuit board 70. The aforementioned reagent preservation device 9 is set, and the cooling sheet 91 and the temperature sensor 92 are used. When the temperature sensor 92 detects that the temperature of the reagent is high, the cooling sheet 91 is used to dissipate heat and cool it, so as to ensure that the reagent is preserved and stored at a lower temperature.

[0077] As described above, the portable immunoblot analyzer provided by the utility model has the following beneficial effects:

[0078] The utility model utilizes the above-mentioned portable immunoblot analyzer. Compared with the prior art, due to the adoption of such a structure, a cleaning device and a membrane detection device with a shooting function are provided. Compared with the existing structure adopting manual cleaning, the cleaning mechanism with a time-limited function can be used to perform time-limited cleaning on the solid phase carrier membrane, and then the cleanliness is detected in combination with the membrane detection device. Only after the cleanliness meets the standard will the solid phase carrier be moved away from the base through the clamping device, thereby eliminating the trouble and labor caused by frequent and repeated cleaning. The utility model has the advantages of automatic cleaning function, high cleaning efficiency and convenient cleaning operation; and it is conducive to the promotion and application of the product.

[0079] The above is only a preferred implementation of the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, which are considered to be within the protection scope of the present invention.

Claims

1. A portable immunoblot analyzer, comprising: A chassis having a machine cavity; A carrier platform, which is used to place a plurality of solid phase carrier membranes made of proteins; It is characterized by: also including: A cleaning device is arranged in the machine cavity, comprising a base with a plurality of upper opening slots and a cleaning mechanism with a time-limited function, wherein the cleaning mechanism is located on the side of the base; A clamping device, which is arranged in the machine cavity and is used to automatically clamp and move the solid phase carrier film placed on the carrier platform to the corresponding upper opening groove; A membrane detection device with a photographing function, which is arranged in the machine cavity and directly opposite to the upper opening groove, and is used to detect the cleanliness of the surface of the solid phase carrier membrane; A control device comprises a circuit board and a cleaning trigger electrically connected to the circuit board, wherein the cleaning device, the clamping device and the membrane detection device are electrically connected to the circuit board respectively; after the cleaning trigger is triggered, the cleaning mechanism performs time-limited cleaning on the solid phase carrier membrane placed in the upper opening groove; after the membrane detection device detects that the cleanliness of the solid phase carrier meets the standard, the clamping device is activated to move the solid phase carrier away from the base.

2. A portable immunoblot analyzer according to claim 1, characterized in that: The cleaning mechanism comprises: A liquid tank containing cleaning liquid and having a water supply port and a water outlet; A liquid pump; the liquid pump is built into the liquid tank, and a signal end of the liquid pump is electrically connected to the circuit board; A liquid pipe, the water inlet end of which extends into the water outlet and communicates with the water through hole of the liquid pump, the liquid pipe is upwardly directed, hidden in the top of the chassis and arranged downwardly along the longitudinal direction; A spray head having a plurality of spray holes, the top end of the spray head being connected to the water outlet end of the liquid pipe, and the spray holes and the upper opening groove being arranged opposite to each other; A timer is integrated in the circuit board, and a signal end of the timer is electrically connected to a signal end of the liquid pump.

3. A portable immunoblot analyzer according to claim 2, characterized in that: Also included is a waste liquid purification mechanism, which has: A waste liquid collection chamber, which is arranged on the base, and the waste liquid collection chamber is connected with each of the upper opening grooves; A waste liquid purification box, one opening of which is connected to the waste liquid collection chamber through a pipeline, and another opening of which is connected to the liquid tank through another pipeline; A liquid purifier is embedded in the waste liquid purification box, and is used to purify the liquid that flows back into the waste liquid purification box.

4. A portable immunoblot analyzer according to claim 3, characterized in that: The liquid purifier is a liquid purifier with a composite filter element and a RO reverse osmosis membrane, and its model is MR1253-A.

5. A portable immunoblot analyzer according to any one of claims 2 to 4, characterized in that: The liquid contained in the liquid tank is pure water.

6. A portable immunoblot analyzer according to claim 1, characterized in that: The cleaning mechanism includes a plasma cleaning structure having: A cleaning box with an air inlet and an air outlet, a positive electrode plate, a negative electrode plate and a vacuum pump, wherein the cleaning box can be turned over to cover the outer peripheral edge of the base, the positive electrode plate is embedded in the left side of the inner cavity of the cleaning box along the longitudinal direction, and the negative electrode plate is embedded in the right side of the inner cavity of the cleaning box along the longitudinal direction; During cleaning, the air inlet is closed, the air suction end of the vacuum pump is connected to the air outlet of the cleaning box, and the positive electrode plate, the negative electrode plate and the power transmission end of the vacuum pump are electrically connected to the circuit board through wires.

7. A portable immunoblot analyzer according to claim 6, characterized in that: The cleaning mechanism also includes a shielding layer, The left and right ends of the shielding layer are in contact between the top of the positive electrode plate and the top of the negative electrode plate, and the shielding layer is attached to the top surface of the inner cavity of the cleaning box.

8. A portable immunoblot analyzer according to claim 1, characterized in that: The clamping device includes a bending seat embedded in the inner wall of the machine cavity, a rotating motor and a clamping hand, the base of the rotating motor is fixed on the horizontal plate of the bending seat, the power transmission end of the rotating motor is electrically connected to the circuit board, and the clamping hand is fixed to the shaft of the rotating motor.

9. A portable immunoblot analyzer according to claim 1, characterized in that: The film detection device comprises a support platform and a shooting camera, wherein the base of the shooting camera is fixed to the support platform; The shooting position of the shooting camera is arranged opposite to the solid phase carrier film placed in the upper opening groove, and the signal end of the shooting camera is electrically connected to the circuit board.

10. A portable immunoblot analyzer according to claim 1, characterized in that: It also includes a reagent preservation device, which includes a coating block coated with a reagent obtained after an immunoassay using a solid phase carrier membrane, a cooling plate and a temperature sensor. The cooling plate is attached to the surface of the coating block, and the temperature sensor is used to detect the temperature of the coating block. The temperature sensor is electrically connected to the circuit board.

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