Automatic sample adding reaction equipment for 96-hole elisa plate

Abstract

The utility model discloses 96-hole elisa plate automatic sample adding reaction equipment, which belongs to the technical field of experimental auxiliary instruments and comprises an operation table, a control panel arranged on the side surface of the operation table, an elisa plate and a storage area arranged on the upper end surface of the operation table, reagents placed in the storage area, and a 360-degree rotating sample injector arranged on the operation table. The sample injector is connected with a sample adding needle; the control panel controls the sample injector to move, the reagents in the storage area are sequentially extracted and dripped into corresponding hole sites of the elisa plate through the sample adding needle according to set types and volumes, and the control panel is operated to heat and uniformly shake the elisa plate. The control panel is used for controlling the sample injector to extract reagents and samples on the object placing table into corresponding hole sites of the elisa plate according to the sample adding sequence, types, volumes and the like according to a set program, and then the clamping positions have the shaking function and the heating constant-temperature control function, so that the reaction requirements of the samples and the reagents can be met; and other auxiliary equipment is not needed.

Description

Technical Field

[0001] The utility model belongs to the technical field of experimental auxiliary instruments, and in particular relates to a 96-well ELISA plate automatic sample addition reaction device. Background Art

[0002] The 96-well ELISA plate is an important consumable for immunological reactions in enzyme-linked immunosorbent assays (ELISA). When using a 96-well ELISA plate to add samples well by well, it is easy to miss or add samples to the wrong wells due to the large number of wells and their density. After adding samples, the ELISA plate needs to be transferred to a flat shaker for mixing, and then transferred to an auxiliary device such as a water bath or incubator for reaction, which is inconvenient to operate.

[0003] The Chinese utility model patent with publication number CN201637743U discloses a highly efficient ELISA plate positioning, oscillation, and incubation all-in-one machine, characterized in that it includes an ELISA plate positioning device, an oscillator device arranged in the incubator base below the ELISA plate positioning device, an incubator device that can heat the ELISA plate, and an incubator base arranged in the incubator base. The three originally independent devices, namely, the ELISA plate positioning device, the oscillation device, and the incubator device, are cleverly integrated into one, which not only greatly reduces the floor space, but also maximizes the use of the limited space on the fully automatic ELISA device, and reduces the frequent transfer of the ELISA plate in the three devices during the test, which can greatly improve the working efficiency and processing capacity of the fully automatic ELISA device. The scheme has proposed to integrate positioning, oscillation, and incubation, but the process of adding samples still requires manual operation, which requires further intelligent improvement. Utility Model Content

[0004] In order to solve the above problems, the utility model aims to provide a 96-well ELISA plate automatic sample loading reaction device.

[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a 96-well ELISA plate automatic sample loading reaction device, comprising an operating table, a control panel is arranged on the side of the operating table, an ELISA plate and a storage area are arranged on the upper end surface of the operating table, reagents are placed in the storage area, and a 360° rotating sample injector is arranged on the operating table, and a sample loading needle is connected to the sample injector;

[0006] The control panel controls the movement of the sample injector, and the reagents in the storage area are sequentially extracted and dripped into the corresponding wells of the ELISA plate according to the set types and volumes through the sample injection needle, and the control panel is operated to heat and shake the ELISA plate.

[0007] Preferably, the injector includes an injection arm with a pipeline arranged inside. One end of the pipeline is arranged in a vessel containing needle washing solution and the other end is connected to the sample adding needle. A pump and a solenoid valve are also arranged inside the injection arm to control the sample adding needle to extract reagents and needle washing solution.

[0008] Preferably, the injection arm is composed of two "L"-shaped frames, one end of one "L"-shaped frame is fitted and fixed to the side end of the operating table, the other end is connected to one end of another "L"-shaped frame and slides left and right on it, and the other end of the other "L"-shaped fixed frame is slidably connected to the sample injection needle, and the sample injection needle slides back and forth on it.

[0009] Preferably, a waste liquid discharge hole is also provided in the moving area of ​​the sample injector on the operating table.

[0010] Preferably, the storage area is arranged in parallel with the ELISA plate, and the items placed therein include but are not limited to reagents and samples.

[0011] Preferably, an ELISA plate fixing clamp is provided below the ELISA plate, the ELISA plate is clamped on the clamp, and a shaking frequency regulating valve is provided on the clamp.

[0012] Preferably, a temperature control plate is provided on the ELISA plate holder, and the temperature of the temperature control plate is set via a control panel.

[0013] Preferably, the control panel is electrically connected to the sample injector, the temperature control plate arranged below the ELISA plate, and the shaking frequency regulating valve.

[0014] Compared with the prior art, the utility model has the following advantages: the utility model controls the sample injector through the control panel to extract the reagents and samples on the storage table into the corresponding wells of the ELISA plate according to the sample addition order, type, volume, etc. according to the set program, and then combines the card position with shaking function and heating function to shake the solution to prevent leakage or wrong well addition. The automatic sample addition reaction equipment has a high degree of automation and intelligence, saving manpower, material resources and time. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the specific embodiments of the present invention, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1 This is a schematic diagram of the automatic sample loading reaction device for 96-well ELISA plates in the utility model;

[0017] Figure 2 This is a schematic diagram of the fixed positioning structure of the ELISA plate in the utility model;

[0018] In the figure, 1-operating table; 2-control panel; 3-ELISA plate; 4-storage area; 5-injector; 51-injection needle; 52-injection arm; 6-vessel; 7-waste liquid discharge hole. DETAILED DESCRIPTION

[0019] The present invention is further described below in conjunction with the accompanying drawings and specific embodiments, but it should not be understood that the scope of the subject matter described in the present invention is limited to the following embodiments. Without departing from the above-mentioned technical ideas of the present invention, various modifications, substitutions and changes made according to the common technical knowledge and customary means in the field are included in the scope of the present invention.

[0020] Reference Figure 1 The 96-well ELISA plate automatic loading reaction device comprises an operating table 1, which is rectangular in shape. A control panel 2 is arranged in front of the operating table 1. An ELISA plate 3 and a storage area 4 are arranged on the upper end surface of the operating table 1. The storage area 4 and the ELISA plate 3 are arranged on a horizontal line. A fixed clamping position of the ELISA plate 3 is arranged below the ELISA plate 3. The ELISA plate 3 is clamped on the clamping position. The clamping position structure refers to Figure 2 The card position is equipped with a shaking frequency regulating valve, and the vibration frequency is adjusted by the shaking frequency regulating valve. A temperature control plate is also provided on the card position, which is arranged at the bottom of the card position. The temperature of the temperature control plate is adjustable, and the temperature control plate heats and controls the temperature of the ELISA plate 3.

[0021] The injector 5 is fixed on the side of the operating table 1, and the injector 5 is suspended above the operating table. The injector 5 includes an injection arm 52, and a pipeline is arranged in the injection arm 52. One end of the pipeline is arranged in the vessel 6 containing the needle washing solution, and the other end is connected to the sample adding needle 51. The injection arm 52 is also equipped with a pump and a solenoid valve to control the sample adding needle 5 to extract reagents and needle washing solution. The injection arm 52 is composed of two "L"-shaped frames, one end of which is fixed on the side of the operating table 1, and the other end is connected to one end of the other "L"-shaped frame and slides left and right thereon. The other end of the other "L"-shaped fixed frame is slidably connected to the sample adding needle 51, and the sample adding needle 51 slides back and forth thereon.

[0022] A waste liquid discharge hole 7 is also provided in the moving area of ​​the sample injector 5 on the operating table 1. The control panel 2 is electrically connected with the sample injector 5, the temperature control plate provided below the ELISA plate 3, and the shaking frequency regulating valve.

[0023] Working process:

[0024] First, the required reagents are placed in the storage area 4 according to different types, and the types and corresponding positions of the reagents in the storage area are input on the control panel 2, and then the volume, type, and order of the reagents to be added to each well of the ELISA plate 3 are set, and finally the vibration frequency and temperature required for the reagents to be shaken after adding the reagents to the ELISA plate 3 are set. Then click to start, and the control panel 2 controls the injection arm 52 on the injector 5 to move forward, backward, left, and right, and the pump configured in the injection arm 52 extracts the reagents and transfers them to the corresponding wells of the ELISA plate 3. After the extraction is completed, the needle washing solution in the vessel 6 is extracted by the pump to flush the pipeline and the injection needle.

[0025] Finally, operate the control panel to enable the shaking frequency regulating valve configured on the ELISA plate 3 to start the shaking function according to the set frequency, and the temperature control plate arranged below the ELISA plate 3 heats the ELISA plate 3 according to the set temperature, and shakes and heats the reagents in the ELISA plate 3, thereby completing automatic sample addition without error or omission.

[0026] When waste liquid appears during the operation, it can be discharged from the waste liquid discharge hole 7, saving process steps.

[0027] The above is a detailed introduction to a 96-well ELISA plate automatic sample loading reaction device provided by the utility model. The structure and working principle of the utility model are described in detail using specific examples. The description of the above embodiments is only used to help understand the method and core idea of ​​the utility model. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the utility model, the utility model can also be improved and modified, and these improvements and modifications also fall within the scope of protection of the claims of the utility model.

Claims

1. A 96-well ELISA plate automatic sample loading reaction device, characterized in that: The operating table (1) comprises a control panel (2) arranged on the side of the operating table (1), an ELISA plate (3) and a storage area (4) arranged on the upper end surface of the operating table (1), reagents are placed in the storage area (4), and a 360° rotatable sample injector (5) is arranged on the operating table (1), and a sample injector (51) is connected to the sample injector (5); The control panel (2) controls the movement of the sample injector (5), and extracts the reagents in the storage area (4) in sequence according to the set types and volumes through the sample injection needle (51) and drips them into the corresponding wells of the ELISA plate (3), and operates the control panel (2) to heat and shake the ELISA plate (3).

2. The 96-well ELISA plate automatic sample loading reaction equipment according to claim 1, characterized in that: The sample injector (5) comprises a sample injection arm (52), wherein a pipeline is arranged in the sample injection arm (52), one end of the pipeline is arranged in a vessel (6) containing a needle washing solution, and the other end is connected to a sample adding needle (51), and a pump and a solenoid valve are also arranged in the sample injection arm (52) to control the sample adding needle (51) to extract reagents and needle washing solution.

3. The 96-well ELISA plate automatic sample loading reaction equipment according to claim 2, characterized in that: The injection arm (52) is composed of two "L"-shaped frames, one end of one of the "L"-shaped frames is fitted and fixed to the side end of the operating table (1), and the other end is connected to one end of the other "L"-shaped frame and slides left and right thereon, and the other end of the other "L"-shaped fixed frame is slidably connected to the injection needle (51), and the injection needle (51) slides back and forth thereon.

4. The 96-well ELISA plate automatic sample loading reaction equipment according to claim 1, characterized in that: A waste liquid discharge hole (7) is also provided in the moving area of ​​the sample injector (5) on the operating table (1).

5. The 96-well ELISA plate automatic sample loading reaction equipment according to claim 1, characterized in that: The storage area (4) is arranged in parallel with the ELISA plate (3), and the items placed therein include reagents and samples.

6. The 96-well ELISA plate automatic sample loading reaction equipment according to claim 1, characterized in that: An enzyme labeling plate (3) fixing clamping position is arranged below the enzyme labeling plate (3), the enzyme labeling plate (3) is clamped on the clamping position, and a shaking frequency regulating valve is arranged on the clamping position.

7. The 96-well ELISA plate automatic sample loading reaction equipment according to claim 6, characterized in that: A temperature control plate is arranged on the ELISA plate holder, and the temperature of the temperature control plate is set through a control panel.

8. The 96-well ELISA plate automatic sample loading reaction equipment according to claim 1, characterized in that: The control panel (2) is electrically connected to the sample injector (5), the temperature control plate arranged below the ELISA plate (3), and the shaking frequency regulating valve.

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