CAR-T cell cryopreservation box

Abstract

The utility model discloses a CAR-T cell cryopreservation box which comprises a box body, a box cover is arranged on the box body, a first partition plate and a second partition plate are sequentially arranged in the box body from bottom to top, an injection pipe is arranged on the box body, a third partition plate is arranged between the first partition plate and the second partition plate, a through hole is formed in the second partition plate, and a through hole is formed in the box cover. A cryopreservation sleeve is arranged in the passing hole in a penetrating mode, and a second air hole is formed in the side wall, located in the branch storage bin, of the cryopreservation sleeve. According to the CAR-T cell cryopreservation box disclosed by the utility model, due to the partition design, each cryopreservation sleeve independently corresponds to one sub-storage bin, so that frozen gas can only enter the sub-storage bins needing cryopreservation, the waste of liquid nitrogen is reduced, and the CAR-T cell cryopreservation box is convenient to use. And meanwhile, the opening degree of the first air hole is adjusted by adjusting the height of the cryopreservation sleeve, so that pre-cooling in advance or maximization of the cryopreservation effect is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of cell cryopreservation, and in particular to a CAR-T cell cryopreservation box. Background Art

[0002] CAR-T cells need to be cryopreserved using liquid nitrogen. Traditional cell cryopreservation boxes have the following problems:

[0003] First, in a cryopreservation box with more than one cryopreservation position, there is no partition between different cryopreservation positions, that is, all cryopreservation positions share the cryopreservation effect, so that the frozen air fills the area. This has no effect when there are frozen cells in all cryopreservation positions, but in actual use, often not all cryopreservation positions are used. In this state, a large cooling area is wasted, that is, liquid nitrogen is wasted;

[0004] Secondly, the opening of the air holes between the liquid nitrogen warehouse and the freezing area is not easy to adjust, that is, the speed at which the frozen air enters the freezing position is not easy to adjust, which is not conducive to pre-cooling the storage position in advance or accurately controlling the freezing effect. Utility Model Content

[0005] In order to solve the deficiencies in the prior art, the utility model provides a CAR-T cell cryopreservation box. The CAR-T cell cryopreservation box is designed through partitions, and each cryopreservation sleeve corresponds to a sub-storage bin separately, so that the frozen gas can only enter the sub-storage bin that needs to be frozen, thereby reducing the waste of liquid nitrogen. At the same time, by adjusting the height of the cryopreservation sleeve, the opening of the first air hole is adjusted to facilitate pre-cooling in advance or maximize the freezing effect.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] The utility model provides a CAR-T cell freezing box, comprising a box body, a box cover is arranged on the box body, a first partition and a second partition are arranged in sequence from bottom to top inside the box body, a liquid nitrogen bin is surrounded between the first partition and the inner wall of the box body, an injection pipe is arranged on the box body and the other end of the injection pipe extends to the liquid nitrogen bin, a third partition is arranged between the first partition and the second partition, and the third partition divides the space between the first partition and the second partition into sub-storage bins;

[0008] A passage hole is provided on the second partition plate corresponding to the sub-storage bin, a freezing sleeve is passed through the passage hole, the freezing sleeve is connected to the inner wall of the passage hole by a thread, one end of the freezing sleeve extends into the sub-storage bin, and a second air hole is provided on the side wall of the freezing sleeve located in the sub-storage bin;

[0009] The first partition is provided with a first air hole corresponding to the sub-storage bin, and a plug is provided at the bottom of the freezing sleeve corresponding to the first air hole.

[0010] The CAR-T cell cryopreservation box of the utility model can inject liquid nitrogen into the liquid nitrogen bin through the injection tube. When the cryopreservation operation is not performed, all the cryopreservation sleeves are at their lowest position. At this time, the plug blocks the first air hole to minimize the waste of liquid nitrogen. When the cryopreservation operation is required, the box cover is opened, and the corresponding cryopreservation sleeve is rotated to raise the sub-storage bin to be used. At this time, the plug slowly leaves the first air hole as the cryopreservation sleeve rises, and the frozen gas enters the corresponding sub-storage bin through the first air hole and enters the cryopreservation sleeve through the second air hole. The temperature inside the cylinder is lowered, and the other unused cryopreservation sleeves remain unchanged. The frozen air is only stored in the cryopreservation sleeves that need to be used. The height to which the cryopreservation sleeve is raised depends on the needs. When pre-cooling operation is required, the cryopreservation sleeve can be raised before placing the cells. The raised height can be smaller so that the frozen air slowly enters the corresponding storage bin to achieve the pre-cooling effect. After the cells are placed in the cryopreservation sleeve, the cryopreservation sleeve needs to be raised to the highest position so that the opening of the cryopreservation sleeve can be blocked when the box lid is closed. At this time, the frozen air enters the storage bin to the greatest extent, achieving the freezing effect.

[0011] The CAR-T cell cryopreservation box of the utility model is designed with partitions, and each cryopreservation sleeve corresponds to a separate storage bin, so that the frozen gas can only enter the storage bin that needs to be frozen, reducing the waste of liquid nitrogen. At the same time, the opening of the first air hole is adjusted by adjusting the height of the cryopreservation sleeve, which is convenient for pre-cooling in advance or maximizing the freezing effect.

[0012] In a further technical solution, the plug and the first air hole are both truncated cone shaped.

[0013] The cooperation between the truncated cone-shaped plug and the first air hole realizes the adjustment of the opening of the first air hole.

[0014] In a further technical solution, a rubber ring is provided on the inner wall of the first air hole.

[0015] The rubber ring can improve the sealing effect, that is, avoid the leakage of frozen gas, and further reduce the waste of liquid nitrogen.

[0016] In a further technical solution, the inner wall of the through hole and the outer wall of the freezing sleeve are respectively provided with rubber rings, and the rubber rings are correspondingly provided with threads.

[0017] The provision of the rubber ring can improve the sealing effect, that is, further avoid the leakage of frozen gas and further reduce the waste of liquid nitrogen.

[0018] In a further technical solution, a prompt area is marked on the rubber ring on the outer wall of the cryopreservation sleeve.

[0019] The function of the prompt area is to prompt the height of the cryopreservation sleeve, which corresponds to the opening of the first air hole, so that the user can control the height of the cryopreservation sleeve more accurately.

[0020] In a further technical solution, a movable handle is provided at the top opening of the cryopreservation sleeve.

[0021] The movable handle can assist the user in rotating the cryopreservation sleeve, thereby preventing the user from directly contacting the cryopreservation sleeve and improving operational safety and convenience.

[0022] In a further technical solution, both ends of the movable handle are connected to the inner wall of the cryopreservation sleeve via a rotating shaft, and a groove for accommodating the movable handle is provided on the top of the cryopreservation sleeve.

[0023] The rotating shafts at both ends of the movable handle and the groove design on the top of the cryopreservation sleeve enable the movable handle to be hidden in the groove when not in use, without affecting the entry and exit of cells or the sealing of the top of the cryopreservation sleeve.

[0024] In a further technical solution, a sealing ring is provided on the box cover at a position corresponding to the freezing sleeve.

[0025] The provision of the sealing ring further improves the sealing effect of the top opening of the cryopreservation sleeve, and further reduces the leakage of frozen gas.

[0026] The beneficial effects are:

[0027] 1. The CAR-T cell cryopreservation box of the utility model is designed by partitioning, and each cryopreservation sleeve corresponds to a separate storage bin, so that the frozen gas can only enter the storage bin that needs to be frozen, reducing the waste of liquid nitrogen. At the same time, the opening of the first air hole is adjusted by adjusting the height of the cryopreservation sleeve, which is convenient for pre-cooling in advance or maximizing the freezing effect.

[0028] 2. The cooperation between the truncated cone-shaped plug and the first air hole realizes the adjustment of the opening of the first air hole.

[0029] 3. The rubber ring can improve the sealing effect, that is, avoid the leakage of frozen gas and further reduce the waste of liquid nitrogen.

[0030] 4. The setting of the rubber ring can improve the sealing effect, that is, further avoid the leakage of frozen gas and further reduce the waste of liquid nitrogen.

[0031] 5. The function of the prompt area is to prompt the height of the cryopreservation sleeve, which corresponds to the opening of the first air hole, so that the user can control the height of the cryopreservation sleeve more accurately.

[0032] 6. The movable handle can assist the user to rotate the cryopreservation sleeve, avoiding direct contact between the user and the cryopreservation sleeve, thus improving operational safety and convenience.

[0033] 7. The rotating shafts at both ends of the movable handle and the groove design on the top of the cryopreservation sleeve allow the movable handle to be hidden in the groove when not in use, without affecting the entry and exit of cells or the sealing of the top of the cryopreservation sleeve.

[0034] 8. The setting of the sealing ring further improves the sealing effect of the top opening of the freezing sleeve and further reduces the leakage of frozen gas. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a schematic diagram of the internal structure of the CAR-T cell freezing box of an embodiment of the utility model;

[0036] Figure 2 It is a schematic structural diagram of the top opening of the cryopreservation sleeve of the CAR-T cell cryopreservation box according to an embodiment of the utility model.

[0037] 10. Box body; 11. Injection tube; 12. First partition; 121. First air hole; 122. Rubber ring; 13. Second partition; 131. Passage hole; 14. Third partition; 15. Liquid nitrogen bin; 16. Sub-storage bin; 17. Insulation layer; 20. Box cover; 21. Sealing ring; 30. Cryopreservation sleeve; 31. Second air hole; 32. Plug; 33. Movable handle. DETAILED DESCRIPTION

[0038] The utility model is further described below in conjunction with the accompanying drawings:

[0039] Example:

[0040] A CAR-T cell cryopreservation box, such as Figure 1 As shown, it includes a box body 10, a box cover 20 is arranged on the box body 10, a first partition 12 and a second partition 13 are arranged in sequence from bottom to top inside the box body 10, a liquid nitrogen bin 15 is surrounded by the first partition 12 and the inner wall of the box body 10, an injection pipe 11 is arranged on the box body 10, and the other end of the injection pipe 11 extends to the liquid nitrogen bin 15, a third partition 14 is arranged between the first partition 12 and the second partition 13, and the third partition 14 divides the space between the first partition 12 and the second partition 13 into a sub-storage bin 16;

[0041] A through hole 131 is formed on the second partition plate 13 corresponding to the sub-storage bin 16, a freezing sleeve 30 is passed through the through hole 131, the freezing sleeve 30 is connected to the inner wall of the through hole 131 by a thread, one end of the freezing sleeve 30 extends into the sub-storage bin 16, and a second air hole 31 is formed on the side wall of the freezing sleeve 30 located in the sub-storage bin 16;

[0042] The first partition 12 is provided with a first air hole 121 corresponding to the sub-storage bin 16 , and a plug 32 is provided at the bottom of the freezing sleeve 30 corresponding to the first air hole 121 .

[0043] The inner wall of the sub-storage bin 16 and the inner wall of the liquid nitrogen bin 15 are both provided with a heat-insulating layer 17 to reduce temperature loss.

[0044] In this embodiment, the cryopreservation sleeve 30 protrudes from the through hole 131 , that is, there is a certain space on the top of the cryopreservation sleeve 30 for the user to manually hold and rotate the cryopreservation sleeve 30 .

[0045] The CAR-T cell freezing box of the utility model can inject liquid nitrogen into the liquid nitrogen bin 15 through the injection tube 11. When the freezing operation is not performed, all the freezing sleeves 30 are at their lowest position. At this time, the plug 32 blocks the first air hole 121 to minimize the waste of liquid nitrogen. When the freezing operation is required, the box cover 20 is opened, and the corresponding freezing sleeve 30 is rotated to raise the sub-storage bin 16 to be used. At this time, the plug 32 slowly leaves the first air hole 121 as the freezing sleeve 30 rises, and the frozen gas enters the corresponding sub-storage bin 16 through the first air hole 121 and passes through the second air hole 31 The freezing sleeve 30 is cooled down inside, while the other unused freezing sleeves 30 remain unchanged, and the frozen air is only stored in the freezing sleeve 30 that needs to be used. The height to which the freezing sleeve 30 is raised depends on the needs. When pre-cooling operation is required, the freezing sleeve 30 can be raised before the cells are placed in, and the raised height can be relatively small so that the frozen air can slowly enter the corresponding sub-storage bin 16 to achieve the pre-cooling effect. After the cells are placed in the freezing sleeve 30, the freezing sleeve 30 needs to be raised to the highest position so that the opening of the freezing sleeve 30 can be blocked when the box cover 20 is closed. At this time, the frozen air enters the sub-storage bin 16 to the greatest extent, thereby achieving the freezing effect.

[0046] The CAR-T cell cryopreservation box of the present invention is designed with partitions, and each cryopreservation sleeve 30 corresponds to a separate storage bin 16, so that the frozen gas can only enter the sub-storage bin 16 that needs to be frozen, reducing the waste of liquid nitrogen. At the same time, the opening of the first air hole 121 is adjusted by adjusting the height of the cryopreservation sleeve 30, which is convenient for pre-cooling in advance or maximizing the freezing effect.

[0047] In another embodiment, if Figure 1 As shown, the plug 32 and the first air hole 121 are both truncated cone shaped.

[0048] The cooperation between the truncated cone-shaped plug 32 and the first air hole 121 enables the adjustment of the opening of the first air hole 121 .

[0049] In another embodiment, if Figure 1 As shown, a rubber ring 122 is disposed on the inner wall of the first air hole 121 .

[0050] The rubber ring 122 can improve the sealing effect, that is, prevent the leakage of frozen gas, and further reduce the waste of liquid nitrogen.

[0051] It is understandable that after the rubber ring 122 is set, the space enclosed by the rubber ring 122 becomes the space into which the plug 32 is inserted, and this space is the first air hole 121 .

[0052] In another embodiment, if Figure 1 As shown, the inner wall of the through hole 131 and the outer wall of the freezing sleeve 30 are respectively provided with rubber rings, and threads are correspondingly provided on the rubber rings.

[0053] The provision of the rubber ring can improve the sealing effect, that is, further avoid the leakage of frozen gas and further reduce the waste of liquid nitrogen.

[0054] In another embodiment, if Figure 1 As shown, a reminder area is marked on the rubber ring of the outer wall of the cryopreservation sleeve 30 .

[0055] The function of the prompt area is to prompt the height of the cryopreservation sleeve 30 to be raised, which corresponds to the opening of the first air hole 121 , so that the user can more accurately control the height of the cryopreservation sleeve 30 .

[0056] The prompt area can be indicated by color or by dot scale.

[0057] In another embodiment, if Figure 1 As shown, a movable handle 33 is provided at the top opening of the cryopreservation sleeve 30 .

[0058] The movable handle 33 can assist the user in rotating the cryopreservation sleeve 30 , thereby preventing the user from directly contacting the cryopreservation sleeve 30 , thereby improving operational safety and convenience.

[0059] In another embodiment, if Figure 1 As shown, both ends of the movable handle 33 are connected to the inner wall of the cryopreservation sleeve 30 through a rotating shaft, and a groove for accommodating the movable handle 33 is provided on the top of the cryopreservation sleeve 30.

[0060] The rotating shafts at both ends of the movable handle 33 and the groove design at the top of the cryopreservation sleeve 30 enable the movable handle 33 to be hidden in the groove when not in use, without affecting the entry and exit of cells or the sealing of the top of the cryopreservation sleeve 30.

[0061] In another embodiment, if Figure 1 As shown, a sealing ring 21 is provided on the box cover 20 at a position corresponding to the freezing sleeve 30 .

[0062] The provision of the sealing ring 21 further improves the sealing effect of the top opening of the cryopreservation sleeve 30 and further reduces the leakage of frozen gas.

[0063] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, and these changes and improvements fall within the scope of the utility model to be protected.

Claims

1. A CAR-T cell freezing box, characterized in that: The invention comprises a box body, wherein a box cover is arranged on the box body, a first partition and a second partition are arranged in sequence from bottom to top inside the box body, a liquid nitrogen bin is surrounded by the first partition and the inner wall of the box body, an injection pipe is arranged on the box body and the other end of the injection pipe extends to the liquid nitrogen bin, a third partition is arranged between the first partition and the second partition, and the third partition divides the space between the first partition and the second partition into sub-storage bins; A passage hole is provided on the second partition plate corresponding to the sub-storage bin, a freezing sleeve is passed through the passage hole, the freezing sleeve is connected to the inner wall of the passage hole by a thread, one end of the freezing sleeve extends into the sub-storage bin, and a second air hole is provided on the side wall of the freezing sleeve located in the sub-storage bin; The first partition is provided with a first air hole corresponding to the sub-storage bin, and a plug is provided at the bottom of the freezing sleeve corresponding to the first air hole.

2. The CAR-T cell cryopreservation box according to claim 1, characterized in that: The plug and the first air hole are both truncated cone shaped.

3. The CAR-T cell freezing box according to claim 1 or 2, characterized in that: The inner wall of the first air hole is provided with a rubber ring.

4. The CAR-T cell cryopreservation box according to claim 1, characterized in that: The inner wall of the through hole and the outer wall of the freezing sleeve are respectively provided with rubber rings, and threads are correspondingly provided on the rubber rings.

5. The CAR-T cell cryopreservation box according to claim 1, characterized in that: A prompt area is marked on the rubber ring on the outer wall of the freezing sleeve.

6. The CAR-T cell cryopreservation box according to claim 1, characterized in that: A movable handle is arranged at the top opening of the freezing sleeve.

7. The CAR-T cell cryopreservation box according to claim 6, characterized in that: The two ends of the movable handle are connected to the inner wall of the freezing sleeve through a rotating shaft, and the top of the freezing sleeve is provided with a groove for accommodating the movable handle.

8. The CAR-T cell cryopreservation box according to claim 1 or 7, characterized in that: A sealing ring is arranged on the box cover at a position corresponding to the freezing sleeve.

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