Space Nano detection chip

Abstract

The utility model relates to the technical field of detection chips, and discloses a space Nano detection chip which comprises a main shell, a reagent cavity is arranged on the surface of the main shell, a detection reagent is arranged in the reagent cavity, a piston piece is arranged in the reagent cavity, the reagent cavity is communicated with a reaction bin through a reagent flow channel, and the reaction bin is communicated with the main shell. The other end of the reaction bin is communicated with an exhaust port, the other end of the exhaust port is communicated with an exhaust groove formed in the surface of the main shell, an exhaust cover plate is arranged in the exhaust groove, one end of the reaction bin is communicated to the back face of the main shell, and a sealing film is arranged on the back face of the main shell. Aiming at the problem that an instrument is inconvenient to operate in a space environment, the whole process can be automatically operated, the chip can realize automatic nucleic acid detection in the space environment through the reagent cavity, the reagent runner and the exhaust cover plate on the premise of unmanned operation, and the use effect is excellent.

Description

Technical Field

[0001] The utility model relates to the technical field of detection chips, in particular to a space Nano detection chip. Background Art

[0002] Nucleic acid detection is widely used in biological pathogen infection, especially viral infection detection. Since the nucleic acid content in the sample to be tested is extremely low, amplification is usually required to obtain sufficient nucleic acid to be tested, and finally fluorescence, colorimetric or potentiometric signals are used as reporting systems. At present, nucleic acid amplification technology includes variable temperature amplification technology such as polymerase chain reaction (PCR), real-time quantitative PCR (qPCR), nested PCR, reverse transcription real-time quantitative PCR (RT-qPCR) and other conventional PCR amplification technologies, and recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP) as the representative isothermal amplification technology and other two categories of nucleic acid amplification technology.

[0003] Nano chip is a microfluidic chip used for Lamp detection, which integrates amplification and detection. The chip is provided with a sample adding port, a flow channel, and a reaction chamber. The sample is added to the chip through a liquid adding device, and the sample enters the reaction chamber of the chip through the flow channel. After the chip is placed in the instrument, the amplification reaction and detection are carried out under the control of the instrument, thereby achieving the purpose of sample detection.

[0004] However, the existing nucleic acid test requires manual placement of the chip into the instrument. In the space environment, it is inconvenient to manually operate the chip. In order to achieve the situation of nucleic acid testing without human operation in the space station environment, the chip uses a serpentine structure, a piston pressing structure and a cover pressing structure to achieve the purpose of automatic nucleic acid testing in the space environment. Utility Model Content

[0005] The purpose of the utility model is to solve the problems existing in the prior art and to propose a space nano detection chip.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] The space Nano detection chip includes a main shell, a reagent cavity is arranged on the surface of the main shell, a detection reagent is arranged in the reagent cavity, a piston is arranged in the reagent cavity, the reagent cavity is connected to a reaction chamber through a reagent flow channel, the other end of the reaction chamber is connected to an exhaust port, the other end of the exhaust port is connected to an exhaust groove arranged on the surface of the main shell, an exhaust cover is arranged in the exhaust groove, a waterproof and breathable membrane is arranged on the bottom of the exhaust cover, one end of the reaction chamber is connected to the back of the main shell, and a sealing membrane is arranged on the back of the main shell.

[0008] Preferably, it also includes a control instrument that is compatible with the space Nano detection chip, and the control instrument includes two up and down moving motors, a heating module and a third moving motor. The driving ends of the two up and down moving motors are respectively connected to the piston member and the exhaust cover plate, so that the piston member and the exhaust cover plate move deep inside the reagent cavity and deep inside the exhaust groove respectively. The heating module is used to heat the reaction chamber, and the third moving motor is used to move the optical module to read the reaction chamber.

[0009] Preferably, a sliding block is provided inside the exhaust groove, sliding blocks are provided on both sides of the exhaust cover plate, and the sliding blocks are slidably connected to the sliding blocks.

[0010] Preferably, an annular protrusion is provided on the outer ring of the piston member, and the outer diameter of the annular protrusion is slightly larger than the inner diameter of the reagent cavity.

[0011] Preferably, a plurality of reaction chambers are provided, and the output end of the reagent flow channel is connected to a plurality of output pipes via a liquid distributor, and the other ends of the plurality of output pipes are respectively connected to a plurality of reaction chambers.

[0012] Preferably, the reagent flow channel is serpentine.

[0013] Preferably, the main shell is box-shaped, the connection between the exhaust groove and the exhaust port is rectangular, and the size of the waterproof breathable membrane is adapted to the connection.

[0014] Compared with the prior art, the beneficial effects of the utility model are:

[0015] The utility model solves the problem of inconvenient instrument operation in a space environment and can be operated automatically throughout the entire process. The chip can realize automatic nucleic acid detection in a space environment without human operation through a reagent cavity, a reagent flow channel and an exhaust cover, and the use effect is excellent. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a three-dimensional schematic diagram of the space nano detection chip proposed by the utility model;

[0017] Figure 2This is a three-dimensional explosion diagram of the space nano detection chip proposed by the utility model;

[0018] Figure 3 This is a side cross-sectional view of the space nano detection chip proposed by the utility model;

[0019] Figure 4 This is a front cross-sectional view of the space nano detection chip proposed by the utility model;

[0020] Figure 5 This is a schematic diagram of the cooperation between the space Nano detection chip and the control instrument proposed in the utility model.

[0021] In the figure: 1. Main shell; 2. Reagent cavity; 3. Reagent flow channel; 4. Reaction chamber; 5. Exhaust port; 6. Exhaust groove; 7. Exhaust cover; 8. Sealing membrane; 9. Control instrument; 10. Up and down moving motor; 11. Heating module; 12. Third moving motor; 13. Optical module; 14. Liquid distributor; 15. Output pipe; 16. Slide groove; 17. Sliding block; 18. Piston; 19. Waterproof and breathable membrane. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0023] Reference Figure 1-Figure 4 , a space nano detection chip comprises a main shell 1, a reagent cavity 2 is arranged on the surface of the main shell 1, a detection reagent is arranged in the reagent cavity 2, a piston 18 is arranged in the reagent cavity 2, the reagent cavity 2 is connected to a reaction chamber 4 through a reagent flow channel 3, the other end of the reaction chamber 4 is connected to an exhaust port 5, the other end of the exhaust port 5 is connected to an exhaust groove 6 arranged on the surface of the main shell 1, an exhaust cover plate 7 is arranged in the exhaust groove 6, a waterproof and breathable membrane 19 is arranged at the bottom of the exhaust cover plate 7, one end of the reaction chamber 4 is connected to the back of the main shell 1, and a sealing membrane 8 is arranged on the back of the main shell 1;

[0024] It also includes a control instrument 9 that is compatible with the space Nano detection chip. The control instrument 9 includes two up and down moving motors 10, a heating module 11 and a third moving motor 12. The driving ends of the two up and down moving motors 10 are respectively connected to the piston member 18 and the exhaust cover plate 7, so that the piston member 18 and the exhaust cover plate 7 move deep into the reagent chamber 2 and deep into the exhaust groove 6 respectively. The heating module 11 is used to heat the reaction chamber 4, and the third moving motor 12 is used to move the optical module 13 to read the reaction chamber 4.

[0025] When using this device:

[0026] (1) First, the reagent is pre-buried in the reagent cavity 2, the reagent flow channel 3 will hinder the flow of the liquid reagent and prevent the liquid from entering the reaction chamber 4, the piston and the exhaust cover plate 7 are placed on the shell, and both parts have pre-compression space. Before the rocket is launched, the chip is placed in the control instrument 9;

[0027] (2) After the control instrument 9 is placed in the rocket and moves to the space station, the control instrument 9 starts the automatic operation program. First, a vertically movable motor 10 presses the piston 18. At this time, the liquid in the reagent chamber 2 is pressed into the reaction chamber 4. Another vertically movable motor 10 presses the exhaust cover plate 7. At this time, the waterproof breathable membrane 19 is pressed by the cover plate to the deep inside of the exhaust groove 6;

[0028] (3) After the above two actions are completed, the control device 9 starts to heat the reaction chamber 4 through the heating module 11, and the reagents will undergo an amplification reaction at this time;

[0029] (4) After heating, the control device 9 drives the optical module 13 to operate through the third moving motor 12, and the optical module 13 reads the optical data in the reaction chamber 4 through the sealing film 8, so as to detect whether the reagent sample is negative or positive;

[0030] Compared with the existing technology, this device solves the problem of inconvenient instrument operation in a space environment and can be operated automatically throughout the process. The above-mentioned structure of the chip can realize automatic nucleic acid detection in a space environment without human operation, and the use effect is excellent.

[0031] In this embodiment, a slider 17 is provided inside the exhaust groove 6, and slide grooves 16 are provided on both sides of the exhaust cover plate 7, and the slide grooves 16 are slidably connected to the slider 17, so as to limit the position of the exhaust cover plate 7 to prevent it from shifting in position when under pressure and causing liquid leakage.

[0032] In this embodiment, the outer ring of the piston 18 is provided with an annular protrusion, and the outer diameter of the annular protrusion is slightly larger than the inner diameter of the reagent chamber 2, which is conducive to ensuring the stability and sealing of the connection between the piston 18 and the reagent chamber 2.

[0033] In this embodiment, a plurality of reaction chambers 4 are provided, and the output end of the reagent flow channel 3 is connected to a plurality of output pipes 15 through a liquid distributor 14, and the other ends of the plurality of output pipes 15 are respectively connected to a plurality of reaction chambers 4, so that after the piston member 18 is pressed together, the liquid in the reagent cavity 2 can flow evenly into the plurality of reaction chambers 4, so as to facilitate the subsequent detection of the plurality of reaction chambers 4 and ensure the accuracy of the data.

[0034] In this embodiment, the reagent flow channel 3 is serpentine.

[0035] In this embodiment, the main shell 1 is boxed, the connection between the exhaust groove 6 and the exhaust port 5 is rectangular, and the size of the waterproof breathable membrane 19 is adapted to the connection, so that the liquid is filled and the gas is completely discharged without residual air to form bubbles, thereby improving the accuracy of subsequent optical detection results.

[0036] Working principle:

[0037] When using this device:

[0038] (1) First, the reagent is pre-buried in the reagent cavity 2. The serpentine reagent flow channel 3 will hinder the flow of the liquid reagent and prevent the liquid from entering the reaction chamber 4. The piston and the exhaust cover plate 7 are placed on the shell. Both parts have pre-compression space. Before the rocket is launched, the chip is placed in the control instrument 9;

[0039] (2) After the control instrument 9 is placed in the rocket and moves to the space station, the control instrument 9 starts the automatic operation program. First, one up-and-down moving motor 10 presses the piston 18. At this time, the liquid in the reagent chamber 2 is pressed into the reaction chamber 4. Another up-and-down moving motor 10 presses the exhaust cover plate 7. At this time, the waterproof breathable membrane 19 is pressed by the exhaust cover plate 7 to the deep inside of the exhaust groove 6;

[0040] (3) After the above two actions are completed, the control device 9 starts to heat the reaction chamber 4 through the heating module 11, and the reagents will undergo an amplification reaction at this time;

[0041] (4) After heating, the control device 9 drives the optical module 13 to operate through the third moving motor 12. The optical module 13 reads the optical data in the reaction chambers 4 through the sealing film 8, thereby detecting whether the reagent sample is negative or positive.

[0042] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A space nano detection chip, comprising a main shell (1), characterized in that: A reagent cavity (2) is arranged on the surface of the main shell (1), a detection reagent is arranged in the reagent cavity (2), a piston member (18) is arranged in the reagent cavity (2), the reagent cavity (2) is connected to a reaction chamber (4) through a reagent flow channel (3), the other end of the reaction chamber (4) is connected to an exhaust port (5), the other end of the exhaust port (5) is connected to an exhaust groove (6) arranged on the surface of the main shell (1), an exhaust cover plate (7) is arranged in the exhaust groove (6), a waterproof and breathable membrane (19) is arranged at the bottom of the exhaust cover plate (7), one end of the reaction chamber (4) is connected to the back of the main shell (1), and a sealing membrane (8) is arranged on the back of the main shell (1).

2. The space nano detection chip according to claim 1, characterized in that: It also includes a control instrument (9) matched with the space nano detection chip, the control instrument (9) includes two up and down moving motors (10), a heating module (11) and a third moving motor (12), the driving ends of the two up and down moving motors (10) are respectively connected to the piston member (18) and the exhaust cover plate (7), so that the piston member (18) and the exhaust cover plate (7) move respectively to the deep inside of the reagent chamber (2) and the deep inside of the exhaust groove (6), the heating module (11) is used to heat the reaction chamber (4), and the third moving motor (12) is used to move the optical module (13) to read the reaction chamber (4).

3. The space nano detection chip according to claim 2, characterized in that: A sliding block (17) is arranged inside the exhaust groove (6), sliding blocks (16) are arranged on both sides of the exhaust cover plate (7), and the sliding blocks (16) are slidably connected to the sliding blocks (17).

4. The space nano detection chip according to claim 2, characterized in that: The outer ring of the piston member (18) is provided with an annular protrusion, and the outer diameter of the annular protrusion is slightly larger than the inner diameter of the reagent chamber (2).

5. The space nano detection chip according to claim 2, characterized in that: The reaction chambers (4) are provided in plurality, and the output end of the reagent flow channel (3) is connected to a plurality of output pipes (15) via a liquid distributor (14), and the other ends of the plurality of output pipes (15) are respectively connected to the plurality of reaction chambers (4).

6. The space nano detection chip according to claim 1, characterized in that: The reagent flow channel (3) is serpentine.

7. The space nano detection chip according to claim 2, characterized in that: The main shell (1) is box-shaped, the connection between the exhaust groove (6) and the exhaust port (5) is rectangular, and the size of the waterproof breathable membrane (19) is adapted to the connection.

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