Orifice plate suspension cell culture box
By designing the well plate suspension cell culture incubator, the problem of cumbersome steps caused by the weight of the well plate mixer is solved, and the convenience of cell suspension culture and constant environment growth are achieved.
Patent Information
- Application Number
- CN202421089682.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-17
- Publication Date
- 2025-01-21
- Estimated Expiration
- 2034-05-17
AI Technical Summary
In the prior art, the orifice plate mixer is heavier, which causes the experimenter to lift it into a carbon dioxide constant temperature box to be cumbersome, which is not conducive to improving the convenience of cell suspension culture.
A well plate suspension cell culture box is designed, including a box body, a box cover, a temperature control device, a carbon dioxide control device and a shaker. The experimenter only needs to place the orifice plate on the positioning fixture inside the box. After the box lid is closed, the system automatically adjusts the temperature and carbon dioxide concentration, and shakes the orifice plate through a shaker to achieve uniform mixing of cells and culture medium.
This device simplifies the steps of cell suspension culture, improves the convenience of experiments, eliminates the need to carry a well plate mixer, and ensures that cells grow in a constant temperature and carbon dioxide environment.
Smart Images
Figure CN222374668U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a well plate suspension cell culture box. Background Art
[0002] In cell culture, cells need to be in a constant temperature and gas environment to grow normally. Carbon dioxide and temperature play an important role in the process of cell culture. Carbon dioxide can maintain the pH value of the culture medium, provide the carbon source required by the cells, and promote the metabolic activity of the cells. Well plate suspension cell culture is a commonly used method in cell culture. Cells are added to the culture medium in a suspended state. It is particularly suitable for multi-factor exploration experiments. This method usually uses well plates to culture cells. Well plates are an experimental device used for cell culture. They are usually made of transparent plastic or glass and have multiple small holes. These small holes are used to separate different cell samples or experimental conditions, so that multiple experiments can be carried out simultaneously on the same culture plate, such as cell proliferation experiments, drug screening, gene expression analysis, etc.
[0003] In order to suspend the cells in the well plate, the experimenter usually lifts the well plate mixer into the carbon dioxide incubator first, and then places the well plate inoculated with cells on the well plate mixer. Then the carbon dioxide incubator is closed and the well plate mixer is started. Under the action of the well plate mixer, the cells and culture fluid in the well plate can be fully mixed, and the cells in the well plate can be suspended and cultured in a growth environment with a constant temperature and constant carbon dioxide. Since the well plate mixer is heavy, the steps for the experimenter to lift the well plate mixer into the carbon dioxide incubator are rather cumbersome, which is not conducive to improving the convenience of the experimenter in cell suspension culture. Utility Model Content
[0004] The utility model aims to solve at least one of the technical problems existing in the prior art. To this end, the utility model provides a well plate suspension cell culture box, which is conducive to improving the convenience of experimenters in cell suspension culture.
[0005] The well plate suspension cell culture box according to the embodiment of the utility model comprises:
[0006] A box body and a box cover, wherein the box cover is hinged to the front end of the box body so that the box cover can be turned downward to open the box body, or the box cover can be turned upward to close the box body;
[0007] A temperature control device, wherein the temperature control device is provided with a preset temperature value, and the temperature control device is used to control the temperature of the inner cavity of the box so that the temperature of the inner cavity of the box can be equal to the preset temperature value;
[0008] A carbon dioxide control device, wherein the carbon dioxide control device is provided with a preset carbon dioxide concentration value, and the carbon dioxide control device is used to control the carbon dioxide concentration in the inner cavity of the box so that the carbon dioxide concentration in the inner cavity of the box can be equal to the preset carbon dioxide concentration value;
[0009] A shaking table and a plurality of positioning and clamping jigs, wherein the shaking table is arranged in the box body, the shaking table is arranged on the lower side wall of the box body, the plurality of positioning and clamping jigs are all used to clamp the orifice plate, the plurality of positioning and clamping jigs are all detachably connected to the shaking table, and the shaking table is used to drive the plurality of positioning and clamping jigs to shake, so that the positioning and clamping jigs can drive the clamped orifice plate to shake.
[0010] At least the following beneficial effects:
[0011] When the experimenter needs to place the orifice plate on the shaker in the box, the experimenter can flip the box cover downward to make the box cover away from the box and open the box. Then the experimenter can hold the orifice plate and place it directly on the positioning clamping fixture on the shaker. The positioning clamping fixture can clamp the orifice plate so that the orifice plate can be connected to the shaker through the positioning clamping fixture. After the orifice plate is placed on the positioning clamping fixture, the experimenter flips the box cover upward to cover the box cover on the box and close the box, so that the inner cavity of the box forms a closed space. Then the temperature control device, the carbon dioxide control device and the shaker are started. Under the action of the temperature control device, the temperature control device can adjust the temperature of the inner cavity of the box body so that the temperature of the inner cavity of the box body is equal to the preset temperature value, so that the cells in the well plate can be in a suitable growth temperature environment; the carbon dioxide control device can adjust the carbon dioxide concentration in the inner cavity of the box body so that the carbon dioxide concentration in the inner cavity of the box body can be equal to the preset carbon dioxide concentration value, so that the cells in the well plate can be in a suitable growth carbon dioxide concentration environment; the shaker drives the positioning clamping fixture to shake, and under the clamping action of the positioning clamping fixture, the shaker can drive the well plate on the positioning clamping fixture to shake, so that the culture fluid and cells in the well plate can be shaken and evenly mixed. After the cell culture is completed, the experimenter only needs to open the box cover and directly remove the well plate from the positioning clamping fixture to remove the well plate from the box. The well plate suspension cell culture box can shake the well plate and provide a culture environment with a constant temperature and a constant carbon dioxide concentration for the cells in the well plate. The experimenter only needs to place the well plate on the positioning clamping fixture in the box, without the need to carry the well plate mixer, which simplifies the steps for the experimenter to perform cell suspension culture and helps to improve the convenience of the experimenter to perform cell suspension culture.
[0012] According to the well plate suspension cell culture incubator of the embodiment of the utility model, the shaker includes a shake plate and a slide plate driving mechanism, the output end of the slide plate driving mechanism is connected to the shake plate, and multiple positioning clamping jigs can be detachably connected to the shake plate, and the slide plate driving mechanism is used to simultaneously drive the shake plate to move in the front-to-back direction and the left-to-right direction, so that the shake plate can drive the positioning clamping jig and the well plate on the positioning clamping jig to shake.
[0013] According to the well plate suspension cell culture box of the embodiment of the utility model, the shaker also includes an adapter, which is connected to the output end of the slide drive mechanism, and the shake plate can be slidably connected to the adapter along the front and rear directions so that the shake plate can extend out of the box body.
[0014] According to the well plate suspension cell culture incubator of the embodiment of the utility model, the shaker also includes a fixing component, and the fixing component is used to fix the shake plate on the adapter.
[0015] According to the well plate suspension cell culture incubator of the embodiment of the utility model, the fixing assembly includes a screw and a nut, an avoidance groove is opened on the rocking plate, one end of the screw is rotatably connected to the adapter so that the other end of the screw can swing into the avoidance groove, and the nut is threadedly connected to the other end of the screw so that the nut can be pressed against the upper surface of the rocking plate and the rocking plate is fixed to the adapter.
[0016] According to the well plate suspension cell culture box of the embodiment of the utility model, a handle is provided on the front end of the rocking plate.
[0017] According to the well plate suspension cell culture incubator of the embodiment of the utility model, the positioning clamping fixture includes a base plate and two positioning enclosures, the base plate is detachably connected to the rocking plate, the two positioning enclosures are both arranged on the base plate, the area between the two positioning enclosures is used to accommodate the well plate, and the two positioning enclosures can abut against the well plate.
[0018] According to the well plate suspension cell culture incubator of the embodiment of the utility model, one of the positioning enclosures is provided with a pushing assembly, and the pushing assembly is used to push the well plate between two of the positioning enclosures so that the well plate can be pressed against the other positioning enclosure.
[0019] According to the well plate suspension cell culture incubator of the utility model embodiment, the pushing assembly includes a pushing member and an elastic member, wherein a guide hole parallel to the left and right directions is opened on one of the positioning enclosures, the pushing member can be slidably arranged in the guide hole along the left and right directions, and a partial structure of the pushing member can extend out of the guide hole, and a guide chamfer is provided at the upper end of the pushing member, and the guide chamfer is used to abut against the well plate, so that the well plate can be arranged between the two positioning enclosures and the pushing member abuts against the plate cover and the plate base of the well plate, the elastic member is arranged in the guide hole, and the two ends of the elastic member are respectively connected to the pushing member and the inner wall of the guide hole, and the elastic member is used to push the pushing member in the direction of the other positioning enclosure, so that the plate cover and the plate base of the well plate can be pressed against the other positioning enclosure.
[0020] According to the well plate suspension cell culture box of the embodiment of the utility model, it also includes a lighting lamp and an ultraviolet lamp. The lighting lamp and the ultraviolet lamp are both arranged in the box body. The lighting lamp and the ultraviolet lamp are both arranged on the left wall or the right wall of the box body. The lighting lamp is used to illuminate the inner cavity of the box body, and the ultraviolet lamp is used to irradiate the inner cavity of the box body.
[0021] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] The present invention is further described below with reference to the accompanying drawings and embodiments, wherein:
[0023] Figure 1 This is a schematic diagram of the structure of the orifice plate of the utility model embodiment;
[0024] Figure 2 This is a structural schematic diagram of a plate cover in an orifice plate of an embodiment of the utility model in an open state;
[0025] Figure 3 This is a schematic diagram of the structure of a well plate suspension cell culture box according to an embodiment of the utility model;
[0026] Figure 4 This is a structural schematic diagram of a well plate suspension cell culture box in an embodiment of the utility model, in which the box cover is in an open state;
[0027] Figure 5 for Figure 4 A schematic diagram of the local enlarged structure at point A in the middle;
[0028] Figure 6 for Figure 4 A schematic diagram of the local enlarged structure at B in the middle;
[0029] Figure 7 This is a schematic diagram of the structure of a shaker, a plurality of positioning clamping fixtures and a plurality of well plates in a well plate suspension cell culture box according to an embodiment of the utility model;
[0030] Figure 8 This is a schematic diagram of the structure of the positioning clamping fixture and the well plate in the well plate suspension cell culture box according to the embodiment of the utility model;
[0031] Fig. 9 This is a schematic diagram of the structure of a positioning and clamping fixture in a well plate suspension cell culture box according to an embodiment of the utility model;
[0032] Fig.10 for Fig. 9 A schematic diagram of the local enlarged structure at C in the middle;
[0033] Reference numerals:
[0034] Box cover 100; observation window 110;
[0035] Box body 200; lighting lamp tube 210; ultraviolet lamp tube 220;
[0036] Rocking bed 300; rocking plate 310; avoidance groove 311; handle 312; threaded hole 313; adapter 320; slide plate driving mechanism 330; fixing assembly 340; screw rod 341; nut 342; connecting member 350;
[0037] Positioning clamping fixture 400; base plate 410; positioning enclosure 420; pushing assembly 430; pushing member 431; guide chamfer 432. DETAILED DESCRIPTION
[0038] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.
[0039] In the description of the present invention, it should be understood that descriptions involving orientation, such as up, down, front, back, left, right, etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.
[0040] In the description of the present utility model, "a plurality" means more than two. If there is a description of "first" or "second", it is only for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.
[0041] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, connecting, etc. should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.
[0042] refer to Figures 1 to 4 According to the embodiment of the utility model, the well plate suspension cell culture box includes:
[0043] A box body 200 and a box cover 100, wherein the box cover 100 is hinged to the front end of the box body 200, so that the box cover 100 can be turned downward to open the box body 200, or the box cover 100 can be turned upward to close the box body 200;
[0044] A temperature control device, wherein the temperature control device is provided with a preset temperature value, and the temperature control device is used to control the temperature of the inner cavity of the box body 200 so that the temperature of the inner cavity of the box body 200 can be equal to the preset temperature value;
[0045] A carbon dioxide control device, wherein the carbon dioxide control device is provided with a preset carbon dioxide concentration value, and the carbon dioxide control device is used to control the carbon dioxide concentration in the inner cavity of the box 200 so that the carbon dioxide concentration in the inner cavity of the box 200 can be equal to the preset carbon dioxide concentration value;
[0046] A shaking table 300 and a plurality of positioning and clamping jigs 400, wherein the shaking table 300 is arranged in the box body 200, and the shaking table 300 is arranged on the lower side wall of the box body 200, and the plurality of positioning and clamping jigs 400 are all used to clamp the orifice plate, and the plurality of positioning and clamping jigs 400 can be detachably connected to the shaking table 300, and the shaking table 300 is used to drive the plurality of positioning and clamping jigs 400 to shake, so that the positioning and clamping jigs 400 can drive the clamped orifice plate to shake.
[0047] It is understandable that when the experimenter needs to place the orifice plate on the shaking table 300 in the box 200, the experimenter can flip the box cover 100 downwards so that the box cover 100 is away from the box 200 and open the box 200, and then the experimenter can hold the orifice plate and place the orifice plate directly on the positioning clamping fixture 400 on the shaking table 300. The positioning clamping fixture 400 can clamp the orifice plate so that the orifice plate can be connected to the shaking table 300 through the positioning clamping fixture 400. After the orifice plate is placed on the positioning clamping fixture 400, the experimenter flips the box cover 100 upwards so that the box cover 100 covers the box 200 and closes the box 200, so that the inner cavity of the box 200 forms a closed space. Then the temperature control device, the carbon dioxide control device and the shaking table 300 are started. Under the action of the temperature control device, the temperature control device can adjust the temperature of the inner cavity of the box 200 so that the temperature of the inner cavity of the box 200 is equal to the preset temperature value, so that the cells in the well plate can be in a suitable growth temperature environment; the carbon dioxide control device can adjust the carbon dioxide concentration in the inner cavity of the box 200 so that the carbon dioxide concentration in the inner cavity of the box 200 is equal to the preset carbon dioxide concentration value, so that the cells in the well plate can be in a suitable growth carbon dioxide concentration environment; the shaking table 300 drives the positioning clamping fixture 400 to shake. Under the clamping action of the positioning clamping fixture 400, the shaking table 300 can drive the well plate on the positioning clamping fixture 400 to shake, so that the culture fluid and cells in the well plate can be shaken and evenly mixed. After the cell culture is completed, the experimenter only needs to open the box cover 100 and directly remove the well plate from the positioning clamping fixture 400 to remove the well plate from the box 200. The well plate suspension cell culture box can shake the well plate and provide a culture environment with a constant temperature and a constant carbon dioxide concentration for the cells in the well plate. The experimenter only needs to place the well plate on the positioning clamping fixture 400 in the box body 200, without having to carry the well plate mixer, which simplifies the steps for the experimenter to perform cell suspension culture and helps to improve the convenience of the experimenter to perform cell suspension culture.
[0048] refer to Figure 4 , Figure 5 and Figure 7 The rocking table 300 includes a rocking plate 310 and a slide driving mechanism 330. The output end of the slide driving mechanism 330 is connected to the rocking plate 310. A plurality of positioning and clamping fixtures 400 are detachably connected to the rocking plate 310. The slide driving mechanism 330 is used to simultaneously drive the rocking plate 310 to move in the front-to-back direction and the left-to-right direction, so that the rocking plate 310 can drive the positioning and clamping fixture 400 and the well plate on the positioning and clamping fixture 400 to rock. It can be understood that the slide driving mechanism 330 can drive the rocking plate 310 to move in the front-to-back direction and the left-to-right direction at the same time. Under the combination of the two movement directions, the rocking plate 310 can be rocked in the horizontal direction to achieve the purpose of fully mixing the cells and the culture medium in the well plate.
[0049] refer to Figure 4 and Figure 5 The shaking table 300 further includes an adapter 320, which is connected to the output end of the slide driving mechanism 330, and the shaking plate 310 can be connected to the adapter 320 in a sliding manner in the front-to-back direction, so that the shaking plate 310 can extend out of the box 200. It can be understood that the shaking plate 310 is connected to the output end of the slide driving mechanism 330 through the adapter 320. When the well plate needs to be placed on the positioning clamping fixture 400, or when the well plate needs to be removed from the positioning clamping fixture 400, the experimenter can first flip down the box cover 100. Since the shaking plate 310 can be connected to the adapter 320 in a sliding manner in the front-to-back direction, the experimenter can move the shaking plate 310 forward and extend out of the box 200. After the shaking plate 310 and the multiple positioning clamping fixtures 400 on the shaking plate 310 extend out of the box 200, it is convenient for the experimenter to place and remove the well plate, thereby improving the convenience of using the well plate suspension cell culture box.
[0050] refer to Figure 4 and Figure 5 The rocking bed 300 further includes a fixing assembly 340, which is used to fix the rocking plate 310 on the adapter 320. After the rocking plate 310 extends out of the box 200, the rocking plate 310 needs to be pushed back into the box 200. The fixing assembly 340 can fix the rocking plate 310 on the adapter 320, thereby preventing the rocking plate 310 from moving relative to the adapter 320 during the rocking process, thereby improving the stability of the operation of the rocking bed 300.
[0051] refer to Figure 5 The fixing assembly 340 includes a screw rod 341 and a nut 342. The rocking plate 310 is provided with an escape groove 311. One end of the screw rod 341 is rotatably connected to the adapter 320 so that the other end of the screw rod 341 can swing into the escape groove 311. The nut 342 is threadedly connected to the other end of the screw rod 341 so that the nut 342 can press against the upper surface of the rocking plate 310 and fix the rocking plate 310 on the adapter 320. It can be understood that when the rocking plate 310 needs to be pulled forward from the box body 200, the experimenter can loosen the nut 342 so that the nut 342 is separated from the abutment with the upper surface of the rocking plate 310, and then rotate the screw rod 341 so that the other end of the screw rod 341 leaves the escape groove 311 on the rocking plate 310. At this time, the rocking plate 310 can be loosened so that the rocking plate 310 can slide forward. When the rocking plate 310 needs to be fixed on the adapter 320, the experimenter can move the rocking plate 310 backward, and then rotate the screw rod 341 so that the other end of the screw rod 341 swings into the avoidance groove 311 on the rocking plate 310. Then the experimenter can tighten the nut 342 so that the nut 342 presses against the upper surface of the rocking plate 310, thereby fixing the rocking plate 310 on the adapter 320.
[0052] refer to Figure 7 A handle 312 is provided on the front end of the rocking plate 310. It is understandable that the handle 312 on the front end of the rocking plate 310 can be held by the experimenter, so that the experimenter can control the rocking plate 310 to move forward or backward, thereby improving the convenience of using the well plate suspension cell culture box.
[0053] refer to Figure 8 and Fig. 9 The positioning clamping fixture 400 includes a base plate 410 and two positioning enclosures 420. The base plate 410 is detachably connected to the rocking plate 310. The two positioning enclosures 420 are both arranged on the base plate 410. The area between the two positioning enclosures 420 is used to accommodate the orifice plate, and the two positioning enclosures 420 can abut against the orifice plate. It can be understood that the base plate 410 is detachably connected to the rocking plate 310, and the two positioning enclosures 420 are both arranged on the base plate 410. The experimenter can place the orifice plate between the two positioning enclosures 420. The two positioning enclosures 420 can abut against the orifice plate, and the two positioning enclosures 420 play a role in positioning the orifice plate and can drive the orifice plate to shake. Specifically, the two positioning enclosures 420 extend in the front-to-back direction, and the two positioning enclosures 420 are distributed in the left-to-right direction.
[0054] refer to Fig. 9 and Fig.10 , one of the positioning enclosures 420 is provided with a pushing assembly 430, which is used to push the orifice plate between the two positioning enclosures 420, so that the orifice plate can be pressed against the other positioning enclosure 420. It can be understood that the pushing assembly 430 can push the orifice plate between the two positioning enclosures 420, so that the orifice plate can be pressed and fixed on the other positioning enclosure 420, that is, the other positioning enclosure 420 and the pushing assembly 430 play a role in clamping and fixing the orifice plate, avoiding the collision between the orifice plate and the two positioning enclosures 420 during the shaking process, and improving the stability of the orifice plate during the shaking process.
[0055] refer to Fig.10 The pushing assembly 430 includes a pushing piece 431 and an elastic piece, wherein a guide hole parallel to the left and right directions is opened on one of the positioning enclosures 420, and the pushing piece 431 can be slidably arranged in the guide hole along the left and right directions, and a part of the structure of the pushing piece 431 can extend out of the guide hole, and a guide chamfer 432 is provided at the upper end of the pushing piece 431, and the guide chamfer 432 is used to abut against the orifice plate, so that the orifice plate can be arranged between the two positioning enclosures 420, and the pushing piece 431 abuts against the plate cover and the plate base of the orifice plate, and the elastic piece is arranged in the guide hole, and the two ends of the elastic piece are respectively connected to the pushing piece 431 and the inner wall of the guide hole, and the elastic piece is used to push the pushing piece 431 toward the direction of the other positioning enclosure 420, so that the plate cover and the plate base of the orifice plate can be pressed against the other positioning enclosure 420.
[0056] It should be explained that the orifice plate includes a plate base and a plate cover, and the plate base is provided with a plurality of holes for accommodating cells and culture fluids, and the plate cover is used to cover the plate base. It is understandable that under the elastic pushing action of the elastic member, part of the structure of the push member 431 can extend out of the guide hole. When the experimenter needs to place the orifice plate between the two positioning enclosures 420, the orifice plate will abut against the guide chamfer 432 at the upper end of the push member 431 during the downward placement process, so that the push member 431 retracts into the guide hole and compresses the elastic member. After the orifice plate is completely placed between the two positioning enclosures 420, under the elastic force of the elastic member in a compressed state, the elastic member can push the push member 431 in the direction of moving toward the other positioning enclosure 420, so that the push member 431 can push the orifice plate. Since the pushing member 431 is in contact with the plate base and plate cover of the orifice plate, under the pushing force of the pushing member 431, the plate base and plate cover of the orifice plate can be pressed and fixed on another positioning enclosure 420, avoiding relative shaking of the plate base and plate cover during shaking of the orifice plate, which is beneficial to improving the stability of the orifice plate during shaking.
[0057] refer to Figure 7 and Figure 8 As an embodiment of the utility model, the well plate suspension cell culture box also includes a connector 350, a plurality of threaded holes 313 are provided on the rocking plate 310, a through hole is provided on the base plate 410, the connector 350 is penetrated in the through hole and is threadedly connected with the inner wall of the threaded hole 313, and the connector 350 can be pressed against the base plate 410 to fix the base plate 410 on the rocking plate 310. It can be understood that the connector 350 is a bolt, the well plate suspension cell culture box includes a plurality of bolts, four through holes are provided on the base plate 410, wherein four bolts are respectively penetrated in the four through holes and are threadedly connected with the inner walls of the corresponding four threaded holes 313, and the four bolts are tightened, and the nuts 342 of the four bolts are all pressed against the base plate 410, so that the base plate 410 is fixed on the rocking plate 310, so as to complete the installation of the positioning clamping fixture 400 on the rocking table 300. The experimenter can loosen the four bolts to disconnect the base plate 410 from the rocking plate 310 , so as to complete the removal of the positioning and clamping fixture 400 from the rocking table 300 .
[0058] refer to Figure 4 and Figure 6The well plate suspension cell culture box also includes an illumination lamp 210 and an ultraviolet lamp 220. Both the illumination lamp 210 and the ultraviolet lamp 220 are arranged in the box body 200. Both the illumination lamp 210 and the ultraviolet lamp 220 are arranged on the left wall or the right wall of the box body 200. The illumination lamp 210 is used to illuminate the inner cavity of the box body 200, and the ultraviolet lamp 220 is used to irradiate the inner cavity of the box body 200. It can be understood that the illumination lamp 210 can illuminate the inner cavity of the box body 200, and the illumination lamp 210 can provide lighting for the experimenter, so that the experimenter can accurately place the well plate on the positioning clamping fixture 400, and can clearly observe the situation of the inner cavity of the box body 200. The box cover 100 is provided with an ultraviolet lamp 220, and the ultraviolet lamp 220 is used to irradiate the inner cavity of the box body 200. The ultraviolet lamp 220 can generate ultraviolet rays, and the ultraviolet rays irradiated into the inner cavity of the box 200 can kill microorganisms such as bacteria, fungi and viruses in the inner cavity of the box 200, keeping the inner cavity environment of the box 200 clean and sterile, so as to provide a good growth environment for the bacteria in the well plate.
[0059] refer to Figure 1 The box cover 100 is provided with an observation port, and an observation window 110 is provided in the area corresponding to the observation port of the box cover 100, and the observation window 110 is used to close the observation port. It can be understood that the operator can observe the situation in the box body 200 through the observation window 110, so that the experimenter can observe the growth state of the cells in the well plate in real time, which is beneficial for the experimenter to detect the morphology, density, proliferation rate and any abnormal situation of the cells.
[0060] As an embodiment of the utility model, the well plate suspension cell culture box also includes a humidity detection device and a display, the display is arranged in the box 200, the display is arranged on the rear side wall of the box 200, the humidity detection device is used to detect the humidity of the inner cavity of the box 200, and the humidity detection device is electrically connected to the display so that the display can display the humidity value of the inner cavity of the box 200. It can be understood that the humidity detection device can detect the humidity of the inner cavity of the box 200 in real time, and the detected humidity value can be displayed on the display. The experimenter can know the value displayed on the display through the observation window 110 to know the humidity value of the current inner cavity of the box 200. By monitoring the humidity value of the inner cavity of the box 200, it can help maintain a stable culture environment and ensure that the cells can grow normally. The well plate suspension cell culture box also includes a humidity adjustment device, which is provided with a preset humidity value, and the humidity adjustment device is electrically connected to the humidity detection device so that the humidity of the inner cavity of the box 200 can be maintained at the preset humidity value.
[0061] As an embodiment of the utility model, the well plate suspension cell culture box includes a carbon dioxide gas supply mechanism and a carbon dioxide detection mechanism, the carbon dioxide gas supply mechanism is used to supply carbon dioxide gas to the inner cavity of the box body 200, and the carbon dioxide detection mechanism is used to detect the carbon dioxide concentration in the inner cavity of the box body 200. When the carbon dioxide concentration detected by the carbon dioxide detection mechanism is lower than the preset carbon dioxide concentration value, the carbon dioxide gas supply mechanism supplies carbon dioxide gas to the inner cavity of the box body 200, so that the carbon dioxide concentration in the inner cavity of the box body 200 can be maintained at the preset carbon dioxide concentration value. When the carbon dioxide concentration detected by the carbon dioxide detection mechanism is higher than the preset carbon dioxide concentration value, the carbon dioxide gas supply mechanism stops to reduce the concentration of carbon dioxide to reach the preset carbon dioxide concentration value.
[0062] As an embodiment of the utility model, the well plate suspension cell culture box includes a heating mechanism and a temperature detection mechanism, the heating mechanism is used to increase the temperature of the inner cavity of the box body 200, and the temperature detection mechanism is used to detect the temperature of the inner cavity of the box body 200. When the temperature detected by the temperature detection mechanism is lower than the preset temperature value, the heating mechanism increases the temperature of the inner cavity of the box body 200 so that the temperature of the inner cavity of the box body 200 can be maintained at the preset temperature value. When the temperature detected by the temperature detection mechanism is higher than the preset temperature value, the heating mechanism stops heating to reduce the temperature of the inner cavity of the box body 200 to reach the preset temperature value.
[0063] As an embodiment of the utility model, a bracket is provided on the box body 200, and the box body 200 is placed on the ground through the bracket. The bracket can provide a height from the ground for the box body 200 and the box cover 100, so that the box body 200 can be opened when the box cover 100 is flipped downward, that is, the box cover 100 in the opened state can be completely under the box body 200, so that the rocking plate 310 can be smoothly drawn out of the box body 200.
[0064] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.
[0065] Of course, the present invention is not limited to the above-mentioned embodiments, and technicians familiar with the field may make equivalent modifications or substitutions without violating the spirit of the present invention. These equivalent modifications or substitutions are all included in the scope defined by the claims of this application.
Claims
1. A well plate suspension cell culture incubator, characterized in that: include: A box body and a box cover, wherein the box cover is hinged to the front end of the box body so that the box cover can be turned downward to open the box body, or the box cover can be turned upward to close the box body; A temperature control device, wherein the temperature control device is provided with a preset temperature value, and the temperature control device is used to control the temperature of the inner cavity of the box so that the temperature of the inner cavity of the box can be equal to the preset temperature value; A carbon dioxide control device, wherein the carbon dioxide control device is provided with a preset carbon dioxide concentration value, and the carbon dioxide control device is used to control the carbon dioxide concentration in the inner cavity of the box so that the carbon dioxide concentration in the inner cavity of the box can be equal to the preset carbon dioxide concentration value; A shaking table and a plurality of positioning and clamping jigs, wherein the shaking table is arranged in the box body, the shaking table is arranged on the lower side wall of the box body, the plurality of positioning and clamping jigs are all used to clamp the orifice plate, the plurality of positioning and clamping jigs are all detachably connected to the shaking table, and the shaking table is used to drive the plurality of positioning and clamping jigs to shake, so that the positioning and clamping jigs can drive the clamped orifice plate to shake.
2. The well plate suspension cell culture incubator according to claim 1, characterized in that: The rocking table includes a rocking plate and a slide plate driving mechanism, the output end of the slide plate driving mechanism is connected to the rocking plate, and multiple positioning clamping fixtures can be detachably connected to the rocking plate. The slide plate driving mechanism is used to simultaneously drive the rocking plate to move in the front-to-back direction and the left-to-right direction, so that the rocking plate can drive the positioning clamping fixture and the orifice plate on the positioning clamping fixture to rock.
3. The well plate suspension cell culture incubator according to claim 2, characterized in that: The rocking bed also includes an adapter, which is connected to the output end of the slide driving mechanism. The rocking board can be slidably connected to the adapter along the front-back direction so that the rocking board can extend out of the box.
4. The well plate suspension cell culture incubator according to claim 3, characterized in that: The rocking table further comprises a fixing assembly, and the fixing assembly is used for fixing the rocking plate on the adapter.
5. The well plate suspension cell culture incubator according to claim 4, characterized in that: The fixing assembly includes a screw and a nut. An avoidance groove is provided on the rocking plate. One end of the screw is rotatably connected to the adapter so that the other end of the screw can swing into the avoidance groove. The nut is threadedly connected to the other end of the screw so that the nut can be pressed against the upper surface of the rocking plate and fix the rocking plate on the adapter.
6. The well plate suspension cell culture incubator according to claim 3, characterized in that: A handle is arranged on the front end of the rocking plate.
7. The well plate suspension cell culture incubator according to claim 2, characterized in that: The positioning clamping fixture includes a base plate and two positioning enclosures, the base plate is detachably connected to the rocking plate, the two positioning enclosures are both arranged on the base plate, the area between the two positioning enclosures is used to accommodate the orifice plate, and the two positioning enclosures can abut against the orifice plate.
8. The well plate suspension cell culture incubator according to claim 7, characterized in that: A pushing assembly is provided on one of the positioning enclosures, and the pushing assembly is used to push the orifice plate between the two positioning enclosures so that the orifice plate can be pressed against the other positioning enclosure.
9. The well plate suspension cell culture incubator according to claim 8, characterized in that: The pushing assembly includes a pushing member and an elastic member, wherein a guide hole parallel to the left and right directions is opened on one of the positioning enclosures, the pushing member can be slidably arranged in the guide hole along the left and right directions, a partial structure of the pushing member can extend out of the guide hole, a guide chamfer is provided at the upper end of the pushing member, the guide chamfer is used to abut against the orifice plate, so that the orifice plate can be arranged between the two positioning enclosures and the pushing member abuts against the plate cover and the plate base of the orifice plate, the elastic member is arranged in the guide hole, the two ends of the elastic member are respectively connected to the pushing member and the inner wall of the guide hole, and the elastic member is used to push the pushing member toward the direction of the other positioning enclosure, so that the plate cover and the plate base of the orifice plate can be pressed against the other positioning enclosure.
10. The well plate suspension cell culture incubator according to claim 1, characterized in that: It also includes a lighting lamp tube and an ultraviolet lamp tube, which are both arranged in the box body and on the left side wall or the right side wall of the box body. The lighting lamp tube is used to illuminate the inner cavity of the box body, and the ultraviolet lamp tube is used to irradiate the inner cavity of the box body.