Chip clamp

Abstract

The utility model discloses a chip clamp which comprises the components of a base plate assembly which is provided with an accommodating groove for positioning and accommodating a to-be-sealed chip; a first air inlet channel and a first air outlet channel are formed in the cover body, a second air inlet channel and a second air outlet channel are formed in the cover replaceable part, the first end of the second air inlet channel is communicated with the first air inlet channel, and the second end of the second air outlet channel is communicated with the second air outlet channel. The first end of the second air outlet channel communicates with the first air outlet channel, the cover body detachably covers the bottom plate assembly, the cover replaceable part is arranged on the chip in the containing groove in a pressed mode, and the second end of the second air inlet channel communicates with a liquid drop generation hole of the chip in a sealed mode through a sealing gasket. And the second end of the second air outlet channel is hermetically communicated with a reagent hole of the chip through a sealing gasket. And when different chips are replaced, only the cover replaceable part corresponding to the chip needs to be replaced, so that the universality of the chip clamp is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidics, in particular to a chip clamp. Background Art

[0002] Selective gene expression is common in different tissues of an individual and in different cells of the same tissue. This selective expression characterizes the different functions of cells. Single-cell sequencing technology uses gel beads modified with independent codes to encapsulate individual cells into independent droplets. The independent codes on the gel beads capture the mRNA selectively expressed by the cells. After reverse transcription, amplification, interruption, connection of sequencing tags and sequencing, the selective expression data of the cells are finally obtained, which is of great significance for the study and analysis of cell heterogeneity.

[0003] In the process of forming independent encapsulated cells and beads, a pressure control system is needed to provide specified constant or variable pressure for the oil phase (continuous phase), bead phase (one of the dispersed phases) and cell phase (another dispersed phase), so that the continuous phase continuously shears the dispersed phase, and finally obtains droplets of the "oil-in-water" system. Most of the droplets contain a cell and a bead. After specific biochemical operations, the reverse transcription, amplification, interruption, connection of sequencing tags and sequencing of mRNA are completed. Therefore, the formation of "oil-in-water" droplets and their equipment and instruments are one of the most important hardware of the single-cell technology center.

[0004] The existing clamps usually use electrically controlled mechanical components to form a downward pressure action to complete the sealing of the microfluidic chip. This method involves mechanical design and software design, etc., with high costs and complex maintenance. In addition, the clamps can only be adapted to specific microfluidic chips and do not have a wider range of applicability. For users, using different microfluidic chips requires purchasing new equipment, which puts a lot of financial pressure on them.

[0005] In summary, how to improve the applicability of the clamp is an urgent problem to be solved by those skilled in the art. Utility Model Content

[0006] In view of this, an object of the utility model is to provide a chip clamp to improve the applicability of the clamp.

[0007] In order to achieve the above purpose, the utility model provides the following solutions:

[0008] A chip fixture, comprising:

[0009] A base plate assembly, wherein the base plate assembly is provided with a receiving groove for positioning and receiving the chip to be sealed;

[0010] A cover body and a cover replaceable part detachably connected to the cover body, the cover body is respectively provided with a first air inlet channel and a first air outlet channel, the cover replaceable part is respectively provided with a second air inlet channel and a second air outlet channel, the first end of the second air inlet channel is communicated with the first air inlet channel, the first end of the second air outlet channel is communicated with the first air outlet channel, the cover body is detachably covered on the base plate assembly, the cover replaceable part is pressed against the chip in the accommodating groove, and the second end of the second air inlet channel is sealed and communicated with the droplet generation hole of the chip through a sealing gasket, and the second end of the second air outlet channel is used for sealing and communicating with the reagent hole of the chip through the sealing gasket.

[0011] In another specific embodiment, the first air inlet passage comprises:

[0012] A first main air inlet channel, which is arranged to penetrate the side wall of the cover body, and one end of which is used to connect to a positive pressure air source of a to-be-connected instrument, and the other end of which is blocked;

[0013] A first air intake branch channel, the number of the first air intake branch channels is multiple, and the multiple first air intake branch channels are respectively arranged at intervals along the length direction of the first air intake main channel, one end of the first air intake branch channel is connected to the first air intake main channel, and the other end extends to one end of the cover body facing the replaceable cover part, and is connected to the first end of the second air intake channel.

[0014] In another specific embodiment, the first gas outlet channel comprises:

[0015] A first main air outlet channel, wherein the first main air outlet channel is arranged to penetrate the side wall of the cover body, and one end of the first main air outlet channel is used to connect with the instrument to be connected, and the other end is blocked;

[0016] A first air outlet branch channel, the number of the first air outlet branch channels is multiple, and the multiple first air outlet branch channels are respectively arranged at intervals along the length direction of the first air outlet main channel, one end of the first air outlet branch channel is connected to the first air outlet main channel, and the other end extends to one end of the cover body facing the replaceable cover part, and is connected to the first end of the second air outlet channel.

[0017] In another specific embodiment, a positioning groove for positioning the replaceable cover part is formed on the cover body, and the replaceable cover part is detachably installed in the positioning groove by a fastener.

[0018] In another specific embodiment, the base plate assembly comprises:

[0019] A bottom plate, wherein a receiving through hole is formed on the bottom plate;

[0020] The base plate replaceable part is detachably mounted on an end of the base plate away from the cover body, and is used to block one end of the accommodating channel to enclose the accommodating groove.

[0021] In another specific embodiment, a mounting groove surrounding the accommodating through hole is further provided at one end of the base plate facing away from the cover body, and the replaceable part of the base plate can be detachably mounted in the mounting groove.

[0022] In another specific embodiment, a positioning groove is provided on one of the groove wall of the installation groove and the outer wall of the replaceable bottom plate part, and a positioning protrusion which is positioned and plugged into the positioning groove is provided on the other.

[0023] In another specific embodiment, the replaceable bottom plate is provided with an observation hole for observing the chip;

[0024] A first positioning structure for positioning the end surface of the chip is provided at one end of the replaceable bottom plate facing the cover body.

[0025] In another specific embodiment, a second positioning structure for positioning one side of the chip is provided on one side of the hole wall of the accommodating through hole, and a third positioning structure for positioning the other side of the chip is provided on the other side of the hole wall of the accommodating through hole;

[0026] An elastic positioning column for pushing the other side of the chip is installed in the bottom plate, and the elastic positioning column is used to push the chip to one side of the chip to abut against the second positioning structure.

[0027] In another specific embodiment, the chip fixture further comprises a sealing gasket positioning column, a positioning column mounting hole is formed on the bottom plate, the sealing gasket positioning column is plugged into the positioning column mounting hole and is used to position the sealing gasket;

[0028] One side of the cover body is rotatably connected to the base plate assembly, and the other side of the cover body is detachably connected to the base plate assembly via a fastener or a snap-on connection.

[0029] The various embodiments of the present invention can be arbitrarily combined as needed, and the embodiments obtained after these combinations are also within the scope of the present invention and are part of the specific implementation methods of the present invention.

[0030] The chip fixture provided by the utility model, when in use, only needs to place the chip in the receiving groove of the bottom plate assembly, and the cover body is set on the bottom plate assembly, and the cover replaceable part can be pressed on the chip to achieve sealing of the chip. Since the first end of the second air inlet channel opened on the cover replaceable part is connected with the first air inlet channel opened on the cover body, and the second end of the second air inlet channel is connected with the droplet generation hole of the chip through a sealing gasket, therefore, by passing gas into the first air inlet channel on the cover body, it is achieved to provide positive pressure to the chip, maintain the flow of liquid in the chip, and ensure the continuous progress of the experiment; since the first end of the second air outlet channel opened on the cover replaceable part is connected with the first air outlet channel opened on the cover body, and the second end of the second air outlet channel is connected with the reagent hole of the chip through a sealing gasket, therefore, the pressure relief of the droplet generation chamber of the microfluidic control is achieved through the second air outlet channel and the first air outlet channel. When replacing different chips, only the cover replaceable part corresponding to the chip needs to be replaced, thereby improving the versatility of the chip fixture. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any novel labor.

[0032] Figure 1 A schematic diagram of the exploded structure of the chip fixture provided by the utility model in one direction;

[0033] Figure 2 A schematic diagram of the exploded structure of the chip fixture provided by the utility model in another direction;

[0034] Figure 3 A schematic diagram of the three-dimensional structure of the cover provided by the utility model in one direction;

[0035] Figure 4 A schematic diagram of the three-dimensional structure of the cover provided by the utility model in another direction;

[0036] Figure 5 A schematic diagram of the three-dimensional structure of the replaceable cover provided by the utility model;

[0037] Figure 6 A schematic diagram of the three-dimensional structure of the bottom plate provided by the utility model in one angle direction;

[0038] Figure 7 A schematic diagram of the three-dimensional structure of the bottom plate provided by the utility model in another angle direction;

[0039] Figure 8 A schematic diagram of the three-dimensional structure of the replaceable base plate provided by the utility model.

[0040] in, Figure 1-Figure 8 middle:

[0041] Chip fixture 100, base plate assembly 101, base plate 101-1, accommodating through hole 101-1a, mounting groove 101-1b, positioning protrusion 101-1c, second positioning structure 101-1d, third positioning structure 101-1e, positioning column mounting hole 101-1f, second fastening hole 101-1g, second hinge hole 101-1i, positioning mounting hole 101-1j, third connecting hole 101-1k, base plate replaceable part 101-2, positioning groove 101-2a, observation hole 101-2b, first positioning structure 101-2c, fourth connecting hole 101-2d, cover body 102, first air inlet channel 102a, first main air inlet channel 102a-1, first air inlet branch channel 102a-2, first air outlet channel 102b, first main air outlet channel 102b-1, first air outlet branch channel 102b-2, positioning groove 102c, first fastening hole 102d, first hinge hole 102e, first connecting hole 102f, replaceable cover part 103, second air inlet channel 103a, second air outlet channel 103b, annular groove 103c, second connecting hole 103d, sealing gasket positioning column 104, and rotating shaft 105. DETAILED DESCRIPTION

[0042] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making novel work are within the scope of protection of the utility model.

[0043] Combination Figure 1-Figure 8 As shown, the utility model provides a chip clamp 100 to improve the versatility of the chip clamp 100 .

[0044] like Figure 1 and Figure 2 As shown, the chip fixture 100 includes a base plate assembly 101, a cover body 102 and a replaceable cover part 103. Specifically, the base plate assembly 101 is provided with a receiving groove for positioning and receiving the chip to be sealed. The shape of the receiving groove is adapted to the shape of the chip. The chip is a microfluidic chip. Of course, it is also applicable to other types of chips.

[0045] The cover replaceable component 103 is detachably connected to the cover body 102. Specifically, the cover replaceable component 103 can be detachably connected to the cover body 102 by bolts or the like.

[0046] like Figure 3-Figure 5 As shown, the cover body 102 is provided with a first air inlet channel 102a and a first air outlet channel 102b, and the cover replaceable part 103 is provided with a second air inlet channel 103a and a second air outlet channel 103b, the first end of the second air inlet channel 103a is connected to the first air inlet channel 102a, and the first end of the second air outlet channel 103b is connected to the first air outlet channel 102b. It can be understood that the cross-sectional shapes and trajectories of the first air inlet channel 102a, the first air outlet channel 102b, the second air inlet channel 103a and the second air outlet channel 103b are not limited, and are set according to specific needs.

[0047] The cover body 102 is detachably mounted on the base plate assembly 101. Specifically, the cover body 102 can be detachably connected to the base plate assembly 101 by fasteners such as bolts, or can be snap-connected.

[0048] The cover replaceable part 103 is pressed against the chip in the receiving groove, and the second end of the second air inlet channel 103a is sealed and connected to the droplet generation hole of the chip through a sealing gasket, and the second end of the second air outlet channel 103b is sealed and connected to the reagent hole of the chip through a sealing gasket. By introducing gas into the first air inlet channel 102a on the cover body 102, positive pressure is provided to the chip, the liquid flow in the chip is maintained, and the continuous progress of the experiment is ensured; since the first end of the second air outlet channel 103b provided on the cover replaceable part 103 is connected to the first air outlet channel 102b provided on the cover body 102, and the second end of the second air outlet channel 103b is sealed and connected to the reagent hole of the chip through a sealing gasket, the pressure relief of the droplet generation chamber of the microfluidic control is achieved through the second air outlet channel 103b and the first air outlet channel 102b.

[0049] When using the chip fixture 100 provided by the utility model, it is only necessary to place the chip in the receiving groove of the bottom plate assembly 101, and to cover the cover body 102 on the bottom plate assembly 101, and then the replaceable cover part 103 can be pressed on the chip to achieve sealing of the chip. When replacing a different chip, it is only necessary to replace the replaceable cover part 103 corresponding to the chip, thereby improving the versatility of the fixture.

[0050] In some embodiments, the first air inlet channel 102a includes a first air inlet main channel 102a-1 and a first air inlet branch channel 102a-2, wherein the first air inlet channel 102a is disposed through the side wall of the cover body 102. Figure 3 and Figure 4As shown, the cover body 102 is a rectangular plate, and the first air inlet channel 102a extends from one side wall of the cover body 102 to the other side wall arranged oppositely. One end of the first air inlet channel 102a is used to connect to the positive pressure gas source of the instrument to be connected, and the other end is blocked by a plug or the like to prevent air leakage. It can be understood that at both ends of the first air inlet channel 102a, any end can be selected as the end connected to the positive pressure gas source, and the other end is correspondingly blocked, so that when one end of the first air inlet channel 102a is not convenient to connect to the positive pressure gas source, the other end can be connected to the positive pressure gas source to facilitate the input of gas into the first air inlet channel 102a. Of course, the first air inlet channel 102a can also be set as a blind hole opened on one side wall of the cover body 102 and extending to the other side wall of the cover body 102.

[0051] There are multiple first air intake branch channels 102a-2, and the multiple first air intake branch channels 102a-2 are arranged at intervals along the length direction of the first air intake main channel 102a-1. The arrangement of multiple first air intake branch channels 102a-2 realizes dividing one first air intake channel 102a into multiple outlets to connect to the second air intake channel 103a.

[0052] One end of the first air inlet branch channel 102a-2 is connected to the first air inlet main channel 102a-1, and the other end extends to the end of the cover body 102 facing the cover replaceable part 103, and is connected to the first end of the second air inlet channel 103a. It should be noted that the number of the first air inlet branch channels 102a-2 is the same as the number of the second air inlet channels 103a, and they are arranged one by one. The number of the second air inlet channels 103a is the same as the number of the droplet generation holes of the chip, and they are arranged one by one. Of course, the second air inlet channel 103a can also be set to have a structure similar to the first air inlet channel 102a, and the second air inlet channel 103a is set to have one inlet and multiple outlets.

[0053] It can be understood that the number and position of the inlets of the second air inlet channel 103a on the replaceable cover 103 are designed according to the first air inlet branch channel 102a-2 on the cover body 102, and the number and position of the outlets of the second air inlet channel 103a on the replaceable cover 103 are designed according to the droplet generation holes on the corresponding chip. When the chip to be sealed by the chip fixture 100 is different, only the corresponding replaceable cover 103 needs to be replaced.

[0054] It should be noted that the number of the first air intake passages 102a is not limited to one, and may also be set to two or more.

[0055] In some embodiments, the first outlet channel 102b includes a first outlet main channel 102b-1 and a first outlet branch channel 102b-2, wherein the first outlet main channel 102b-1 is set to penetrate the side wall of the cover body 102, and one end of the first outlet main channel 102b-1 is used to connect to the instrument to be connected, and the other end is blocked to prevent air leakage. Figure 3 and Figure 4 As shown, the first outlet main channel 102b-1 is arranged in parallel with the first inlet main channel 102a-1, and the first outlet branch channel 102b-2 is arranged in parallel with the first inlet branch channel 102a-2.

[0056] There are multiple first air outlet branch channels 102b-2, and the multiple first air outlet branch channels 102b-2 are spaced apart along the length direction of the first air outlet main channel 102b-1, that is, the multiple first air outlet branch channels 102b-2 are merged together and discharged through the first air outlet main channel 102b-1.

[0057] One end of the first air outlet branch channel 102b-2 is connected to the first air outlet main channel 102b-1, and the other end extends to the end of the cover body 102 facing the cover replaceable part 103, and is connected to the first end of the second air outlet channel 103b.

[0058] like Figure 3 and Figure 4 As shown, in a specific embodiment of the present invention, the number of first air inlet channels 102a is 3, the number of first air inlet branch channels 102a-2 included in each first air inlet channel 102a is 8, the number of first air outlet channels 102b is 1, and the number of first air outlet branch channels 102b-2 included is 8.

[0059] In some embodiments, a positioning groove 102c is provided on the cover body 102, and the cover replaceable component 103 is detachably installed in the positioning groove 102c by a fastener. Specifically, the positioning groove 102c is adapted to the shape of the cover replaceable component 103 to achieve positioning of the cover replaceable component 103.

[0060] In order to facilitate the detachable connection between the cover replaceable part 103 and the positioning groove 102c, a specific embodiment of the utility model discloses that a first connecting hole 102f is opened in the positioning groove 102c, and a second connecting hole 103d is opened on the cover replaceable part 103. Fasteners such as bolts can be installed in the positioning groove 102c through the first connecting hole 102f and the second connecting hole 103d. Figure 4 As shown, the number of the first connection holes 102f is 6, correspondingly, as Figure 5 As shown, the number of the second connection holes 103d is also 6.

[0061] To facilitate the installation of the gasket, Figure 5 As shown, a specific embodiment of the utility model discloses that an annular groove 103c is provided on the replaceable cover 103, specifically, there are multiple annular grooves 103c, and each outlet of the second air inlet channel 103a is surrounded by one annular groove 103c, and each inlet of the second air outlet channel 103b is surrounded by one annular groove 103c, and the sealing gasket is provided with a sealing ring which is plugged into each annular groove 103c.

[0062] In some embodiments, the base plate assembly 101 includes a base plate 101 - 1 and a base plate replaceable component 101 - 2 . Specifically, the base plate 101 - 1 is a rectangular plate, and a receiving through hole 101 - 1 a is provided on the base plate 101 - 1 .

[0063] The base plate replaceable part 101 - 2 is detachably mounted on one end of the base plate 101 - 1 away from the cover body 102 , and is used to block one end of the accommodating channel to enclose a accommodating groove.

[0064] In this embodiment, the base plate assembly 101 is divided into a base plate 101 - 1 and a base plate replaceable part 101 - 2 , so that different types of chips can be adapted by replacing different types of base plate replaceable parts 101 - 2 , further improving the versatility of the chip fixture 100 .

[0065] In some embodiments, Figure 7 As shown, an installation groove 101-1b surrounding the accommodating through hole 101-1a is also opened at the end of the base plate 101-1 away from the cover body 102, and the base plate replaceable part 101-2 can be detachably installed in the installation groove 101-1b to prevent the base plate replaceable part 101-2 from protruding out of the end of the base plate 101-1 away from the cover body 102.

[0066] The mounting groove 101-1b is adapted to the shape of the base plate replaceable component 101-2 to achieve the positioning of the base plate replaceable component 101-2.

[0067] Specifically, a third connection hole 101-1k is opened in the installation groove 101-1b, and a fourth connection hole 101-2d is opened on the base plate replaceable component 101-2. The screws pass through the fourth connection hole 101-2d and the third connection hole 101-1k in sequence to realize the connection between the base plate replaceable component 101-2 and the base plate 101-1.

[0068] It can be understood that the end of the bottom plate 101 - 1 facing away from the cover body 102 refers to the end of the bottom plate 101 - 1 facing away from the cover body 102 when the chip clamp 100 is in the state where the cover body 102 is covered on the bottom plate assembly 101 .

[0069] In some embodiments, one of the groove wall of the installation groove 101-1b and the outer wall of the bottom plate replaceable component 101-2 is provided with a positioning groove 101-2a, and the other is provided with a positioning protrusion 101-1c that is positioned and plugged with the positioning groove 101-2a. That is, the bottom plate replaceable component 101-2 and the installation groove 101-1b are further quickly positioned through the cooperation of the positioning protrusion 101-1c and the positioning groove 101-2a.

[0070] In order to avoid the problem of incorrect installation direction of the base plate replaceable part 101-2, a specific embodiment of the utility model discloses that a positioning groove 101-2a is provided on one side of the end of the base plate replaceable part 101-2 facing the base plate 101-1, and a positioning protrusion 101-1c is correspondingly provided in the positioning groove 101-2a.

[0071] Furthermore, an observation hole 101-2b is provided on the replaceable bottom plate 101-2, through which the reaction in the chip can be observed to understand the entire microfluidic experiment process. Specifically, the number of observation holes 101-2b can be set to be multiple, which is convenient for observing the reaction at various positions of the chip and also reduces the weight of the chip fixture 100.

[0072] The end of the replaceable bottom plate 101-2 facing the cover 102 is provided with a first positioning structure 101-2c for positioning the end face of the chip. Specifically, the first positioning structure 101-2c may be a positioning protrusion 101-1c, which realizes the positioning of the chip on the one hand and the division of the chip units on the other hand.

[0073] In some embodiments, a second positioning structure 101-1d for positioning one side of the chip is disposed on one side of the hole wall of the through hole 101-1a, and a third positioning structure 101-1e for positioning the other side of the chip is disposed on the other side of the hole wall of the through hole 101-1a. Figure 7 As shown, the second positioning structure 101 - 1 d and the third positioning structure 101 - 1 e are both protrusions, and correspondingly, a positioning groove 101 - 2 a for positioning and plugging is provided on the chip.

[0074] In order to improve the positioning accuracy of the chip, a specific embodiment of the utility model discloses that an elastic positioning column is installed in the bottom plate 101-1 to push the other side of the chip. The elastic positioning column is used to push the chip to one side of the chip to abut against the second positioning structure 101-1d. Specifically, the number of the elastic positioning columns can be set to multiple, such as Figure 6 As shown, positioning and mounting holes 101 - 1 j are opened on the side wall of the bottom plate 101 - 1 , and the number of the positioning and mounting holes 101 - 1 j is the same as the number of the elastic positioning columns, and they are arranged in a one-to-one correspondence.

[0075] The elastic positioning column specifically includes a positioning column and an elastic part. The elastic part is in a compressed state and is installed in the positioning mounting hole 101-1j. One end of the elastic part is connected to the hole wall of the positioning mounting hole 101-1j, and the other end is connected to the positioning column. The positioning column can be slidably installed in the positioning mounting hole 101-1j and abut against the chip.

[0076] In some embodiments, the chip fixture 100 further includes a sealing gasket positioning column 104. The bottom plate 101-1 is provided with a positioning column mounting hole 101-1f. The sealing gasket positioning column 104 is plugged into the positioning column mounting hole 101-1f and is used to position the sealing gasket to achieve the positioning of the sealing gasket. Specifically, the number of the positioning column mounting holes 101-1f is 4, distributed at the four corners of the bottom plate 101-1.

[0077] In some embodiments, one side of the cover body 102 is rotatably connected to the base plate assembly 101. Specifically, a first hinge hole 102e is opened on the cover plate, and a second hinge hole 101-1i is opened on the base plate 101-1. The rotating shaft 105 rotatably connects the cover plate and the base plate 101-1 through the first hinge hole 102e and the second hinge hole 101-1i.

[0078] The other side of the cover 102 is detachably connected to the base plate assembly 101 through fasteners or snap-on connection. Figure 8 As shown, a first fastening hole 102d is formed on the cover body 102, and a second fastening hole 101-1g is formed on the bottom plate 101-1. Fasteners such as bolts pass through the first fastening hole 102d and the second fastening hole 101-1g to achieve a detachable connection between the cover plate and the bottom plate 101-1.

[0079] The chip fixture 100 provided by the utility model only needs to place the independent microfluidic chip into the corresponding position in the chip fixture 100, that is, in the receiving groove, cover the cover 102 of the chip fixture 100, and use a torque wrench to tighten the cover 102 and the bottom plate 101-1 to complete the fixing and sealing of the microfluidic chip. The whole process only needs simple operations to complete the preparation work before the experiment. In addition, the cover replaceable part 103 and the bottom plate replaceable part 101-2 in the chip fixture 100 can be replaced to be suitable for more microfluidic chips with different structures.

[0080] It should be noted that the terms “first” and “second” are used for descriptive purposes only and should not be understood as indicating or implying relative importance.

[0081] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0082] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A chip fixture, characterized in that: include: A base plate assembly, wherein the base plate assembly is provided with a receiving groove for positioning and receiving the chip to be sealed; A cover body and a cover replaceable part detachably connected to the cover body, the cover body is respectively provided with a first air inlet channel and a first air outlet channel, the cover replaceable part is respectively provided with a second air inlet channel and a second air outlet channel, the first end of the second air inlet channel is communicated with the first air inlet channel, the first end of the second air outlet channel is communicated with the first air outlet channel, the cover body is detachably covered on the base plate assembly, the cover replaceable part is pressed against the chip in the accommodating groove, and the second end of the second air inlet channel is sealed and communicated with the droplet generation hole of the chip through a sealing gasket, and the second end of the second air outlet channel is used for sealing and communicating with the reagent hole of the chip through the sealing gasket.

2. The chip holder according to claim 1, characterized in that: The first air inlet passage comprises: A first main air inlet channel, which is arranged to penetrate the side wall of the cover body, and one end of which is used to connect to a positive pressure air source of a to-be-connected instrument, and the other end of which is blocked; A first air intake branch channel, the number of the first air intake branch channels is multiple, and the multiple first air intake branch channels are respectively arranged at intervals along the length direction of the first air intake main channel, one end of the first air intake branch channel is connected to the first air intake main channel, and the other end extends to one end of the cover body facing the replaceable cover part, and is connected to the first end of the second air intake channel.

3. The chip holder according to claim 2, characterized in that: The first air outlet channel comprises: A first main air outlet channel, wherein the first main air outlet channel is arranged to penetrate the side wall of the cover body, and one end of the first main air outlet channel is used to connect with the instrument to be connected, and the other end is blocked; A first air outlet branch channel, the number of the first air outlet branch channels is multiple, and the multiple first air outlet branch channels are respectively arranged at intervals along the length direction of the first air outlet main channel, one end of the first air outlet branch channel is connected to the first air outlet main channel, and the other end extends to one end of the cover body facing the replaceable cover part, and is connected to the first end of the second air outlet channel.

4. The chip holder according to claim 1, characterized in that: The cover body is provided with a positioning groove for positioning the replaceable cover part, and the replaceable cover part is detachably installed in the positioning groove through a fastener.

5. The chip holder according to claim 1, characterized in that: The base plate assembly comprises: A bottom plate, wherein a receiving through hole is formed on the bottom plate; The base plate replaceable part is detachably mounted on an end of the base plate away from the cover body, and is used to block one end of the accommodating through hole to enclose the accommodating groove.

6. The chip holder according to claim 5, characterized in that: An installation groove surrounding the accommodating through hole is also provided at one end of the bottom plate away from the cover body, and the replaceable part of the bottom plate can be detachably installed in the installation groove.

7. The chip holder according to claim 6, characterized in that: A positioning groove is provided on one of the groove wall of the installation groove and the outer wall of the replaceable bottom plate part, and a positioning protrusion which is positioned and plugged into the positioning groove is provided on the other.

8. The chip holder according to claim 5, characterized in that: The replaceable bottom plate is provided with an observation hole for observing the chip; A first positioning structure for positioning the end surface of the chip is provided at one end of the replaceable bottom plate facing the cover body.

9. The chip holder according to claim 5, characterized in that: A second positioning structure for positioning one side of the chip is disposed on one side of the hole wall of the accommodating through hole, and a third positioning structure for positioning the other side of the chip is disposed on the other side of the hole wall of the accommodating through hole; An elastic positioning column for pushing the other side of the chip is installed in the bottom plate, and the elastic positioning column is used to push the chip to one side of the chip to abut against the second positioning structure.

10. The chip holder according to any one of claims 1 to 9, characterized in that: It also includes a sealing gasket positioning column, the bottom plate is provided with a positioning column mounting hole, the sealing gasket positioning column is plugged into the positioning column mounting hole, and is used to position the sealing gasket; One side of the cover body is rotatably connected to the base plate assembly, and the other side of the cover body is detachably connected to the base plate assembly via a fastener or a snap-on connection.

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