Small nucleic acid solid-phase synthesis device

Abstract

The utility model discloses a small nucleic acid solid-phase synthesis device, which relates to the technical field of biosynthesis and comprises a nucleic acid solid-phase synthesis machine shell and a reagent bottle fixing mechanism, the reagent bottle fixing structure comprises a clamping plate, four pairs of fixing clamping plates and springs, the clamping plate is slidably connected to the bottom wall of the nucleic acid solid-phase synthesizer shell, four round holes are formed in the top of the clamping plate, the four pairs of fixing clamping plates are slidably connected into the four round holes respectively, triangular clamping grooves are formed in the surfaces of the four pairs of fixing clamping plates, and the springs are arranged in the triangular clamping grooves. When the reagent bottle moves downwards to the bottom wall of the round hole, the two springs can release elastic force and push the two fixed clamping plates to slide relatively, and the two fixed clamping plates are clamped on the surface of the reagent bottle through the triangular clamping grooves. Therefore, the reagent bottles with different diameters and sizes can be clamped and fixed through the two fixing clamping plates, and then the reagent bottles with different sizes can be conveniently placed.

Description

Technical Field

[0001] The utility model relates to the technical field of biosynthesis, in particular to a small nucleic acid solid phase synthesis device. Background Art

[0002] Nucleic acid is a biological macromolecule composed of many nucleotides. It is not only an indispensable substance for protein biosynthesis, but also the material basis of biological heredity. The relative molecular mass of nucleic acid is very large. It is widely present in all animal and plant cells and microorganisms. The nucleic acid in organisms often combines with protein to form nucleoprotein.

[0003] The currently published patent: CN216260698U discloses a nucleic acid synthesizer, which positions the reagent bottle on the reagent rack by slidingly connecting the reagent rack, making it convenient for the reagent bottle to cooperate with the second pipetting head and to replace the reagent bottle, thereby improving the efficiency of nucleic acid synthesis. The utility model ensures the stability of the reagent rack during the pulling and pulling process through the cooperation of the slide rail, the slide shaft and the support leg, thereby preventing the reagent in the reagent bottle from falling off.

[0004] Based on the above search, it was found that the above patent has the following problems when in use: when the nucleic acid synthesizer is in use, the reagent bottle can be positioned on the reagent rack by slidingly connecting the reagent rack, which is convenient for the reagent bottle to cooperate with the second pipetting head and for the reagent bottle to be replaced. However, the reagent bottle is fixed by being placed in a circular hole. Since the diameter of the circular hole is fixed, the staff cannot adjust the size of the circular hole according to the size of the reagent bottle, which makes it inconvenient to place reagent bottles of different sizes, reducing the practicality of the nucleic acid synthesizer. Utility Model Content

[0005] The utility model aims to solve at least one of the technical problems existing in the prior art and provide a small nucleic acid solid phase synthesis device, which can solve the problem that a reagent bottle is placed in a circular hole for fixing, and since the diameter of the circular hole is fixed, the staff cannot adjust the size of the circular hole according to the size of the reagent bottle, thereby making it inconvenient to place reagent bottles of different sizes, reducing the practicality of the nucleic acid synthesizer.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a small nucleic acid solid phase synthesis device, comprising a nucleic acid solid phase synthesis machine housing and a reagent bottle fixing mechanism; the reagent bottle fixing structure comprises a clamping plate, four pairs of fixed clamping plates and a spring, the clamping plate is slidably connected to the bottom wall of the nucleic acid solid phase synthesis machine housing, four circular holes are opened on the top of the clamping plate, four pairs of fixed clamping plates are respectively slidably connected to the inside of the four circular holes, the surfaces of the four pairs of fixed clamping plates are opened with triangular grooves, the tops of the four pairs of fixed clamping plates are opened with contact inclined surfaces, the spring is fixedly connected to the surface of the fixed clamping plate, and one end of the spring away from the fixed clamping plate is fixedly connected to the inner wall of the clamping plate.

[0007] Preferably, a reagent tube placement plate is installed inside the housing of the nucleic acid solid phase synthesizer, and an infusion tube is installed on the top wall of the housing of the nucleic acid solid phase synthesizer.

[0008] Preferably, a liquid storage tank is fixedly connected to the interior of the nucleic acid solid phase synthesis machine housing, and a movable pipette is installed inside the nucleic acid solid phase synthesis machine housing.

[0009] Preferably, two shaft sleeves are fixedly connected to the surface of the clamping plate, and sliding rods are slidably sleeved inside the two shaft sleeves, and the two sliding rods are fixedly connected to the inside of the housing of the nucleic acid solid phase synthesizer.

[0010] Preferably, the interiors of the two shaft sleeves are slidably connected with cylindrical sliders, and the two cylindrical sliders are fixedly connected to the two sliding rods respectively.

[0011] Preferably, three annular grooves are formed on the surface of the cylindrical slider, and wear-resistant balls are slidably connected inside the three annular grooves, and the surfaces of the wear-resistant balls are in contact with the inner wall of the sleeve.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] (1) In the small nucleic acid solid phase synthesis device, when the reagent bottle moves downward to the bottom wall of the circular hole, the two springs release their elastic force and push the two fixed clamps to slide relative to each other and clamp on the surface of the reagent bottle through the triangular groove. Since the triangular groove can clamp reagent bottles of different sizes, the two fixed clamps can clamp and fix reagent bottles of different diameters, thereby facilitating the placement of reagent bottles of different sizes, thereby improving the practicality of the small nucleic acid solid phase synthesis device.

[0014] (2) In the small nucleic acid solid phase synthesis device, the clamping plate slides and drives the two sleeves to slide outward on the surfaces of the two slide rods, and the sleeves slide outward on the surface of the cylindrical slider. At this time, the sleeves drive the wear-resistant balls to roll through the friction between the sleeves and the wear-resistant balls, and the sliding friction is converted into rolling friction, thereby reducing the resistance of the sleeves to slide on the surface of the cylindrical slider, making it convenient for the staff to remove the clamping plate and replace the reagent bottle. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The utility model is further described below in conjunction with the accompanying drawings and embodiments:

[0016] Figure 1 This is a schematic diagram of the overall structure of a small nucleic acid solid phase synthesis device of the utility model;

[0017] Figure 2 This is a schematic diagram of the internal structure of the housing of the nucleic acid solid phase synthesis machine of the utility model;

[0018] Figure 3It is a schematic diagram of the cross-sectional structure of the clamping plate of the utility model;

[0019] Figure 4 It is a schematic diagram of the internal structure of the shaft sleeve of the utility model.

[0020] Figure numerals: 1. Nucleic acid solid phase synthesis machine housing; 2. Reagent tube placement plate; 3. Infusion tube; 4. Liquid storage tank; 5. Mobile pipette; 6. Clamping plate; 7. Round hole; 8. Fixed splint; 9. Triangular slot; 10. Contact slope; 11. Spring; 12. Bushing; 13. Slide rod; 14. Cylindrical slider; 15. Annular slot; 16. Wear-resistant ball. DETAILED DESCRIPTION

[0021] This section will describe in detail the specific embodiments of the utility model. The preferred embodiments of the utility model are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the utility model, but it cannot be understood as a limitation on the protection scope of the utility model.

[0022] In the description of the present invention, it should be understood that descriptions involving orientation, such as up, down, front, back, left, right, etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0023] In the description of the present utility model, "greater than", "less than", "exceed" etc. are understood as not including the number itself, and "above", "below", "within" etc. are understood as including the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0024] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, connecting, etc. should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.

[0025] See also Figure 1-4The utility model provides a technical solution: a small nucleic acid solid phase synthesis device, comprising a nucleic acid solid phase synthesis machine housing 1 and a reagent bottle fixing mechanism, a reagent tube placement plate 2 is installed inside the nucleic acid solid phase synthesis machine housing 1, an infusion tube 3 is installed on the top wall of the nucleic acid solid phase synthesis machine housing 1, a liquid storage tank 4 is fixedly connected to the inside of the nucleic acid solid phase synthesis machine housing 1, a movable pipette 5 is installed inside the nucleic acid solid phase synthesis machine housing 1, and a reagent bottle fixing structure comprises a clamping plate 6, four pairs of fixed clamping plates 8 and a spring 11, the clamping plate 6 is slidably connected to the bottom wall of the nucleic acid solid phase synthesis machine housing 1, four circular holes 7 are opened on the top of the clamping plate 6, four pairs of fixed clamping plates 8 are respectively slidably connected to the inside of the four circular holes 7, the surfaces of the four pairs of fixed clamping plates 8 are all provided with triangular card grooves 9, the tops of the four pairs of fixed clamping plates 8 are all provided with contact inclined surfaces 10, the spring 11 is fixedly connected to the surface of the fixed clamping plate 8, and one end of the spring 11 away from the fixed clamping plate 8 is fixedly connected to the inner wall of the clamping plate 6;

[0026] When the small nucleic acid solid phase synthesis device is in use, the staff puts the reagent bottle into the circular hole 7, and then uses the mobile pipette 5 to extract the liquid in the reagent bottle and discharge it into the liquid storage box 4. The staff puts the reagent tube into the hole on the surface of the reagent tube placement plate 2. At this time, the infusion tube 3 can extract the liquid in the liquid storage box 4 and discharge it into the reagent tube for solid phase synthesis of nucleic acids. When the reagent bottles are of different sizes, the staff only needs to slide the reagent bottle downward and squeeze the two contact slopes on the top of a pair of fixed clamps 8. At this time, the two fixed clamps 8 are subjected to the test The squeezing effect of the reagent bottle slides on the opposite sides and squeezes the springs 11 on the same side. The two springs 11 exert opposite elastic forces on the two fixed clamps 8. When the reagent bottle moves downward to the bottom wall of the circular hole 7, the two springs 11 can release the elastic force and push the two fixed clamps 8 to slide relative to each other and clamp them on the surface of the reagent bottle through the triangular groove 9. Since the triangular groove 9 can clamp reagent bottles of different sizes, reagent bottles of different diameters can be clamped and fixed by the two fixed clamps 8, thereby facilitating the placement of reagent bottles of different sizes and improving the practicality of the small nucleic acid solid phase synthesis device.

[0027] Two shaft sleeves 12 are fixedly connected to the surface of the clamping plate 6, and slide rods 13 are slidably sleeved inside the two shaft sleeves 12. The two slide rods 13 are fixedly connected to the inside of the nucleic acid solid phase synthesizer housing 1. Cylindrical sliders 14 are slidably connected inside the two shaft sleeves 12. The two cylindrical sliders 14 are respectively fixedly connected to the two slide rods 13. Three annular grooves 15 are opened on the surface of the cylindrical slider 14. Wear-resistant balls 16 are slidably connected inside the three annular grooves 15, and the surfaces of the wear-resistant balls 16 are in contact with the inner walls of the shaft sleeves 12.

[0028] When the staff needs to replace a new reagent bottle, the staff can slide the clamping plate 6 outward and make it slide out of the interior of the nucleic acid solid phase synthesizer housing 1, so that the staff can put the reagent bottle into the inside of the circular hole 7. The clamping plate 6 slides and drives the two sleeves 12 to slide outward on the surfaces of the two sliding rods 13, and the sleeves 12 slide outward on the surface of the cylindrical slider 14. At this time, the sleeves 12 drive the wear-resistant balls 16 to roll through the friction between the sleeves 12 and the wear-resistant balls 16, and the sliding friction is converted into rolling friction, thereby reducing the resistance of the sleeves 12 to slide on the surface of the cylindrical slider 14, so that the staff can remove the clamping plate 6 and replace the reagent bottle.

[0029] Working principle: In the small nucleic acid solid phase synthesis device, after the staff puts the reagent bottle into the circular hole 7, the liquid in the reagent bottle is extracted and discharged into the liquid storage box 4 through the movable pipette 5. The infusion tube 3 can extract the liquid in the liquid storage box 4 and discharge it into the reagent tube for solid phase synthesis of nucleic acids. The staff slides the reagent bottle downward and squeezes the two contact slopes on the top of a pair of fixed clamps 8. At this time, the two fixed clamps 8 slide in opposite directions under the squeezing effect of the reagent bottle and squeeze the springs 11 on the same side. When the reagent bottle moves downward to the bottom wall of the circular hole 7, the two springs 11 can release the elastic The force pushes the two fixed clamps 8 to slide relative to each other and are clamped on the surface of the reagent bottle through the triangular groove 9. The two fixed clamps 8 can clamp and fix reagent bottles of different diameters. The staff slides the clamping plate 6 outward and makes it slide out of the interior of the nucleic acid solid phase synthesizer housing 1. The clamping plate 6 slides and drives the two sleeves 12 to slide outward on the surfaces of the two slide rods 13. The sleeves 12 slide outward on the surface of the cylindrical slider 14. At this time, the sleeves 12 drive the wear-resistant balls 16 to roll through the friction between them, thereby reducing the sliding resistance of the sleeves 12 on the surface of the cylindrical slider 14.

[0030] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge scope of ordinary technicians in the technical field without departing from the purpose of the present invention.

Claims

1. A small nucleic acid solid phase synthesis device, characterized in that: include: Nucleic acid solid phase synthesis machine housing (1) and reagent bottle fixing mechanism; The reagent bottle fixing structure comprises a clamping plate (6), four pairs of fixed clamping plates (8) and a spring (11); the clamping plate (6) is slidably connected to the bottom wall of the nucleic acid solid phase synthesis machine housing (1); four circular holes (7) are provided on the top of the clamping plate (6); the four pairs of fixed clamping plates (8) are respectively slidably connected inside the four circular holes (7); the surfaces of the four pairs of fixed clamping plates (8) are provided with triangular grooves (9); the tops of the four pairs of fixed clamping plates (8) are provided with contact inclined surfaces (10); the spring (11) is fixedly connected to the surface of the fixed clamping plate (8); and one end of the spring (11) away from the fixed clamping plate (8) is fixedly connected to the inner wall of the clamping plate (6).

2. A small nucleic acid solid phase synthesis device according to claim 1, characterized in that: A reagent tube placement plate (2) is installed inside the nucleic acid solid phase synthesis machine housing (1), and a liquid infusion tube (3) is installed on the top wall of the nucleic acid solid phase synthesis machine housing (1).

3. A small nucleic acid solid phase synthesis device according to claim 1, characterized in that: A liquid storage tank (4) is fixedly connected to the interior of the nucleic acid solid phase synthesis machine housing (1), and a movable pipette (5) is installed inside the nucleic acid solid phase synthesis machine housing (1).

4. A small nucleic acid solid phase synthesis device according to claim 1, characterized in that: Two shaft sleeves (12) are fixedly connected to the surface of the clamping plate (6), and sliding rods (13) are slidably sleeved inside the two shaft sleeves (12), and the two sliding rods (13) are fixedly connected to the inside of the nucleic acid solid phase synthesis machine housing (1).

5. A small nucleic acid solid phase synthesis device according to claim 4, characterized in that: The insides of the two shaft sleeves (12) are both slidably connected with cylindrical sliders (14), and the two cylindrical sliders (14) are fixedly connected to the two sliding rods (13) respectively.

6. A small nucleic acid solid phase synthesis device according to claim 5, characterized in that: The surface of the cylindrical slider (14) is provided with three annular grooves (15), the interiors of the three annular grooves (15) are all slidably connected with wear-resistant balls (16), and the surfaces of the wear-resistant balls (16) are in contact with the inner wall of the shaft sleeve (12).

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