Device for separating and extracting vesicles in human dental pulp mesenchymal stem cells

Abstract

The utility model relates to the technical field of medical instruments, and provides a device for separating and extracting vesicles in human dental pulp mesenchymal stem cells, which comprises an extraction box, a feeding mechanism is arranged above the inside of the extraction box, the bottom of the feeding mechanism is fixedly connected with a funnel, the funnel is inserted into a centrifugal rotating box body, and the centrifugal rotating box body is fixedly connected with the feeding mechanism. An annular supporting rotating plate is fixedly connected to the bottom of the funnel, balls are rotatably connected to the interior of the annular supporting rotating plate, the annular supporting rotating plate and the balls are located in the centrifugal rotating box body, and a first discharging valve and a second discharging valve are fixedly connected to the bottom of the centrifugal rotating box body; the turning of the centrifugal rotating box body can be realized by starting the turning mechanism, so that the vesicles in the centrifugal rotating box body and the separation liquid with different masses can be centrifugally separated, and the vesicle separation substances and the mixed liquid on the upper layer and the lower layer are sequentially discharged through the first discharge valve and the second discharge valve.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, and in particular to a device for separating and extracting vesicles in human dental pulp mesenchymal stem cells. Background Art

[0002] Certain amphiphilic molecules, such as many natural and synthetic surfactants and phospholipids that cannot simply associate into micelles, will spontaneously form a class of ordered molecular assemblies with a closed double-layer structure when dispersed in water, called vesicles or liposomes. The meanings of the two terms vesicle and liposome are somewhat ambiguous in the literature. It is generally believed that if these amphiphilic molecules are the natural surfactant lecithin, the structure formed is called a liposome; if they are composed of synthetic surfactants, they are called vesicles. Therefore, vesicles play an important role as transport carriers in the excretion of secretory proteins.

[0003] After searching, the existing patent (CN221662899U) discloses a cell vesicle extraction device, including a top cover, a bearing is fixedly installed inside the upper top wall of the top cover, and a cell vesicle extraction mechanism is arranged at the bottom of the top cover. The cell vesicle extraction mechanism includes a motor fixing frame fixedly installed on the top of the top cover, a driving motor is fixedly installed inside the motor fixing frame, a middle sleeve is threadedly installed inside the top cover, a mounting plate is fixedly installed inside the middle sleeve, a transmission rod that passes through the bottom of the mounting plate is fixedly installed at the output end of the driving motor, a rotating paddle is set at the bottom of the transmission rod, a dropper is fixedly installed inside the mounting plate, an external conduit that passes through the right side of the middle sleeve is fixedly installed on the top of the dropper, a liquid storage bottle is threadedly installed inside the middle sleeve, and a vesicle filter plate is fixedly installed inside the liquid storage bottle. The utility model removes the liquid storage bottle installed inside the middle sleeve to pour the cell fluid on the top of the liquid storage bottle, then connects the external catheter and the external flushing fluid and sprays it on the vesicle filter plate, then drives the motor to drive the transmission rod to drive the rotating paddle to rotate, stirs the cell fluid, and as the flushing fluid dilutes the cell fluid, the cell fluid flows to the inside of the liquid storage bottle for storage. At this time, the vesicles remain on the top of the vesicle filter plate, and then the liquid storage bottle is rotated to remove the vesicles on the top of the vesicle filter plate, thereby achieving high efficiency and labor-saving operation.

[0004] However, in the above scheme, the stirring by rotating the paddle is not conducive to the integrity of the vesicles, and the filtered and separated liquid and the vesicles are located in the same container, which is inconvenient to take out. The lack of temperature control during the extraction process is not conducive to the preservation of cells.

[0005] In view of this, the utility model proposes a device for separating and extracting vesicles in human dental pulp mesenchymal stem cells. Utility Model Content

[0006] The utility model proposes a device for separating and extracting vesicles in human dental pulp mesenchymal stem cells, which solves the problems in the related art that rotating stirring by rotating paddles is not conducive to the integrity of the vesicles, the filtered and separated liquid and the vesicles are located in the same container and are not convenient to take out, and the lack of temperature control during the extraction process is not conducive to the preservation of cells.

[0007] The technical scheme of the utility model is as follows: a device for separating and extracting vesicles in human dental pulp mesenchymal stem cells, comprising an extraction box: a feeding mechanism is arranged above the interior of the extraction box, a funnel is fixedly connected to the bottom of the feeding mechanism, the funnel is inserted into the interior of a centrifugal rotating box, an annular support rotating plate is fixedly connected to the bottom of the funnel, a ball is rotatably connected to the interior of the annular support rotating plate, the annular support rotating plate and the ball are both located inside the centrifugal rotating box, a first discharge valve and a second discharge valve are fixedly connected to the bottom of the centrifugal rotating box, a steering mechanism is arranged at the bottom of the centrifugal rotating box, and a lower portion of one side of the extraction box is fixedly connected to the feed mechanism. A material collection mechanism is provided, which includes a layered extraction drawer, two material collection slots are provided inside the layered extraction drawer, and the sides of the two material collection slots are provided with a first discharge valve. A U-shaped card plate is provided at the bottom of the extraction box, and a heating wire is provided inside the U-shaped card plate. After the feeding mechanism feeds, the vesicle mixed liquid enters the interior of the centrifugal rotating box through the funnel, and the centrifugal rotating box can be turned by starting the steering mechanism, so that the vesicles and separation liquids of different masses inside the centrifugal rotating box can be centrifugally separated, and the vesicle separations and the mixed liquids of the upper and lower layers are discharged in sequence by the first discharge valve and the second discharge valve.

[0008] Preferably, a liquid inlet drawer is plugged and connected to one side of the top of the extraction box, a liquid outlet valve is fixedly connected to the bottom of the liquid inlet drawer, an electric push rod is fixedly connected to one side of the top of the extraction box, a push plate is fixedly connected to the extended end of the electric push rod, a touch sensor is fixedly connected to one side of the push plate, and the touch sensor is located on one side of the extraction box. By inserting the liquid inlet drawer into the interior of the extraction box, the equipment can be started after the extraction box and the push plate are fitted together, and the liquid inlet drawer can be pushed out by the electric push rod after discharge is completed.

[0009] Preferably, a barrier ring is provided at the bottom of the liquid inlet drawer, the push plate and the liquid inlet drawer are both slidably fitted on the top of the discharge port, a discharge port is opened inside the barrier ring, and the liquid outlet valve is located at the center position of the top of the discharge port.

[0010] Preferably, the funnel is inserted into the center position inside the top plate of the centrifugal rotating box, and the centrifugal rotating box is located at the bottom of the annular support rotating plate and rotates in engagement, and the centrifugal rotating box is limited by the annular support rotating plate and the ball bearings, thereby enabling the centrifugal rotating box to be positioned.

[0011] Preferably, the extraction box is a box structure combined with upper and lower sides, and a hinge is provided in the middle of one side of the extraction box. The upper part of the extraction box is rotated by the hinge. By rotating the upper part of the extraction box, the centrifugal rotating box body and the steering mechanism can be separated, thereby achieving cleaning of the inside of the extraction box.

[0012] Preferably, the steering mechanism includes a motor, a rotating shaft, a gear and an annular tooth groove. One side of the extraction box is fixedly connected to a side box body, the inside of the side box body is fixedly connected to a motor, the output end of the motor is fixedly connected to a rotating shaft, the other end of the rotating shaft is fixedly connected to a gear, and an annular tooth groove is provided at the bottom of the centrifugal rotating box body. The gear is meshed with the annular tooth groove, and the rotation of the centrifugal rotating box body can be realized by the arranged gear being able to mesh with one end of the annular tooth groove.

[0013] Preferably, the upper and lower sides of the side box body are fixedly connected with a liquid storage tank, and the liquid storage tank is connected to the inside of the funnel through a hose. The liquid storage tank can be used to add mixed liquid into the centrifugal rotating box body to cooperate with the separation operation.

[0014] Preferably, the feeding mechanism and the layered extraction drawer are both located on the upper and lower sides of the same side inside the extraction box.

[0015] The beneficial effects of the utility model are:

[0016] After the feeding mechanism of the utility model feeds, the vesicle mixed liquid enters the interior of the centrifugal rotating box through the funnel, and the centrifugal rotating box can be turned by starting the steering mechanism, so that the vesicles and separation liquids of different masses in the centrifugal rotating box can be centrifugally separated, and the vesicle separations and the mixed liquid of the upper and lower layers are discharged in sequence by the first discharge valve and the second discharge valve;

[0017] In the utility model, the liquid inlet drawer is inserted into the inside of the extraction box, and the extraction box and the push plate are fitted to start the equipment. After the discharge is completed, the liquid inlet drawer can be pushed out by the electric push rod, and the centrifugal rotating box body is limited by the annular support rotating plate and the ball, so that the centrifugal rotating box body can be positioned;

[0018] In the utility model, the centrifugal rotating box body and the steering mechanism can be separated by rotating the extraction box on the upper part, so that the inside of the extraction box can be cleaned. The arranged gear can mesh with one end of the annular tooth groove to realize the rotation of the centrifugal rotating box body. The arranged liquid storage tank can add mixed liquid into the inside of the centrifugal rotating box body to cooperate with the separation operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The utility model is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0020] Figure 1 It is a three-dimensional structural schematic diagram of the utility model from a side view;

[0021] Figure 2 It is a three-dimensional structural schematic diagram of another side view of the utility model;

[0022] Figure 3 It is a three-dimensional schematic diagram of the utility model when viewed from above;

[0023] Figure 4 It is a schematic diagram of the internal structure of the utility model from the front;

[0024] Figure 5 For this utility model Figure 4 Schematic diagram of the enlarged structure at point A in the middle.

[0025] In the figure: 1. extraction box; 2. liquid inlet drawer; 3. liquid outlet valve; 4. electric push rod; 5. push plate; 6. touch sensor; 7. barrier ring; 8. discharge port; 9. funnel; 10. annular support rotating plate; 11. ball bearing; 12. centrifugal rotating box; 13. first discharge valve; 14. second discharge valve; 15. side box; 16. liquid storage box; 17. motor; 18. rotating shaft; 19. gear; 20. U-shaped card plate; 21. heating wire; 22. annular tooth groove; 23. layered extraction drawer. DETAILED DESCRIPTION

[0026] The following will be combined with the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] Example 1

[0028] The preferred embodiment of the device for separating and extracting vesicles in human dental pulp mesenchymal stem cells provided by the utility model is as follows: Figures 1 to 5As shown: A device for separating and extracting vesicles in human dental pulp mesenchymal stem cells, comprising an extraction box 1: a feeding mechanism is arranged above the interior of the extraction box 1, a funnel 9 is fixedly connected to the bottom of the feeding mechanism, the funnel 9 is inserted into the interior of a centrifugal rotating box 12, an annular support rotating plate 10 is fixedly connected to the bottom of the funnel 9, a ball 11 is rotatably connected to the interior of the annular support rotating plate 10, the annular support rotating plate 10 and the ball 11 are both located inside the centrifugal rotating box 12, a first discharge valve 13 and a second discharge valve 14 are fixedly connected to the bottom of the centrifugal rotating box 12, a steering mechanism is arranged at the bottom of the centrifugal rotating box 12, a collection mechanism is arranged below one side of the extraction box 1, the collection mechanism comprises a layered extraction drawer 23, two collection slots are arranged inside the layered extraction drawer 23, the sides of the two collection slots are both provided with the first discharge valve 13, a U-shaped card plate 20 is arranged at the bottom of the extraction box 1, a heating wire 21 is arranged inside the U-shaped card plate 20.

[0029] It should be noted that the existing extraction equipment still has certain shortcomings. The rotating stirring by rotating blades is not conducive to the integrity of the vesicles, and the filtered and separated liquid and the vesicles are located in the same container, which is inconvenient to take out. The lack of temperature control during the extraction process is not conducive to cell preservation.

[0030] In this embodiment, after the feeding mechanism feeds, the vesicle mixed liquid enters the interior of the centrifugal rotating box 12 through the funnel 9, and the centrifugal rotating box 12 can be turned by starting the steering mechanism, so that the vesicles and separation liquids of different masses inside the centrifugal rotating box 12 can be centrifugally separated, and the upper and lower layers of vesicle separations and mixed liquids are discharged in sequence by the first discharge valve 13 and the second discharge valve 14.

[0031] In a further preferred embodiment of the utility model, one side of the top of the extraction box 1 is plugged and connected with a liquid inlet drawer 2, the bottom of the liquid inlet drawer 2 is fixedly connected with a liquid outlet valve 3, one side of the top of the extraction box 1 is fixedly connected with an electric push rod 4, the extended end of the electric push rod 4 is fixedly connected with a push plate 5, one side inside the push plate 5 is fixedly connected with a touch sensor 6, and the touch sensor 6 is located on one side of the extraction box 1.

[0032] In this embodiment, the liquid inlet drawer 2 is inserted into the extraction box 1, and the equipment can be started after the extraction box 1 and the push plate 5 are fitted together. After the discharge is completed, the liquid inlet drawer 2 can be pushed out by the electric push rod 4.

[0033] In a further preferred embodiment of the utility model, a barrier ring 7 is provided at the bottom of the liquid inlet drawer 2, the push plate 5 and the liquid inlet drawer 2 both slide in contact with the top of the discharge port 8, a discharge port 8 is provided inside the barrier ring 7, and the liquid outlet valve 3 is located at the center position of the top of the discharge port 8.

[0034] In a further preferred embodiment of the present invention, the funnel 9 is inserted into the center position of the top plate of the centrifugal rotating box 12, and the centrifugal rotating box 12 is located at the bottom of the annular supporting rotating plate 10 and rotates in close proximity thereto.

[0035] In this embodiment, the centrifugal rotating housing 12 is limited by the annular supporting rotating plate 10 and the balls 11, so that the centrifugal rotating housing 12 can be positioned.

[0036] Example 2

[0037] Based on Example 1, a preferred embodiment of the device for separating and extracting vesicles in human dental pulp mesenchymal stem cells provided by the present invention is as follows: Figures 1 to 5 As shown: the extraction box 1 is a box structure combined with upper and lower sides. A hinge is provided in the middle of one side of the extraction box 1, and the upper part of the extraction box 1 rotates through the hinge.

[0038] In this embodiment, the centrifugal rotating box 12 and the steering mechanism can be separated by rotating the upper half of the extraction box 1, so that the inside of the extraction box 1 can be cleaned.

[0039] In a further preferred embodiment of the utility model, the steering mechanism includes a motor 17, a rotating shaft 18, a gear 19 and an annular tooth groove 22. One side of the extraction box 1 is fixedly connected to the side box body 15, the inside of the side box body 15 is fixedly connected to the motor 17, the output end of the motor 17 is fixedly connected to the rotating shaft 18, and the other end of the rotating shaft 18 is fixedly connected to the gear 19. An annular tooth groove 22 is provided at the bottom of the centrifugal rotating box body 12, and the gear 19 is meshed with the annular tooth groove 22.

[0040] In this embodiment, the gear 19 is arranged to mesh with one end of the annular tooth groove 22, so that the centrifugal rotating housing 12 can be rotated.

[0041] In a further preferred embodiment of the present invention, a liquid storage tank 16 is fixedly connected to both the upper and lower sides of the inside of the side box body 15, and the liquid storage tank 16 is connected to the inside of the funnel 9 through a hose.

[0042] In this embodiment, the liquid storage tank 16 is provided to add the mixed liquid into the centrifugal rotating box 12 to cooperate with the separation operation.

[0043] In a further preferred embodiment of the present invention, the feeding mechanism and the layered extraction drawer 23 are both located on the upper and lower sides of the same side inside the extraction box 1 and can be pulled out.

[0044] The working principle of the utility model is as follows: the liquid inlet drawer 2 is inserted into the extraction box 1, and the equipment can be started after the extraction box 1 is fitted with the push plate 5. After the discharge is completed, the liquid inlet drawer 2 can be pushed out by the electric push rod 4. After the feeding, the vesicle mixed liquid enters the centrifugal rotating box 12 from the funnel 9, and then the starting gear 19 is engaged with one end of the annular tooth groove 22 to rotate the centrifugal rotating box 12 to realize the steering of the centrifugal rotating box 12, thereby realizing the centrifugal separation of the vesicles and separation liquids of different qualities inside the centrifugal rotating box 12, and the first discharge valve 13 and the second discharge valve 14 successively discharge the upper and lower layers of vesicle separations and the mixed liquid into the stratified extraction drawer 23.

[0045] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A device for separating and extracting vesicles from human dental pulp mesenchymal stem cells, characterized in that: The invention comprises an extraction box (1): a feeding mechanism is arranged above the inside of the extraction box (1); a funnel (9) is fixedly connected to the bottom of the feeding mechanism; the funnel (9) is inserted into the inside of a centrifugal rotating box (12); an annular support rotating plate (10) is fixedly connected to the bottom of the funnel (9); a ball (11) is rotatably connected to the inside of the annular support rotating plate (10); the annular support rotating plate (10) and the ball (11) are both located inside the centrifugal rotating box (12); the bottom of the centrifugal rotating box (12) is fixedly connected to the A first discharge valve (13) and a second discharge valve (14) are connected, a steering mechanism is arranged at the bottom of the centrifugal rotating box (12), a material collecting mechanism is arranged below one side of the extraction box (1), the material collecting mechanism comprises a layered extraction drawer (23), two material collecting slots are arranged inside the layered extraction drawer (23), the sides of the two material collecting slots are both provided with the first discharge valve (13), a U-shaped card plate (20) is arranged at the bottom of the extraction box (1), and a heating wire (21) is arranged inside the U-shaped card plate (20).

2. The device for separating and extracting vesicles from human dental pulp mesenchymal stem cells according to claim 1, characterized in that: One side of the top of the extraction box (1) is plugged and connected to a liquid inlet drawer (2), the bottom of the liquid inlet drawer (2) is fixedly connected to a liquid outlet valve (3), one side of the top of the extraction box (1) is fixedly connected to an electric push rod (4), the extended end of the electric push rod (4) is fixedly connected to a push plate (5), one side of the interior of the push plate (5) is fixedly connected to a touch sensor (6), and the touch sensor (6) is located on one side of the extraction box (1).

3. The device for separating and extracting vesicles from human dental pulp mesenchymal stem cells according to claim 2, characterized in that: A barrier ring (7) is provided at the bottom of the liquid inlet drawer (2), the push plate (5) and the liquid inlet drawer (2) are both slidably fitted on the top of the discharge port (8), a discharge port (8) is provided inside the barrier ring (7), and the liquid outlet valve (3) is located at the center of the top of the discharge port (8).

4. The device for separating and extracting vesicles from human dental pulp mesenchymal stem cells according to claim 1, characterized in that: The funnel (9) is inserted into the center position of the top plate of the centrifugal rotating box (12), and the centrifugal rotating box (12) is located at the bottom of the annular supporting rotating plate (10) and rotates in close contact.

5. The device for separating and extracting vesicles from human dental pulp mesenchymal stem cells according to claim 1, characterized in that: The extraction box (1) is a box structure composed of upper and lower sides. A hinge is provided in the middle of one side of the extraction box (1), and the upper half of the extraction box (1) rotates via the hinge.

6. The device for separating and extracting vesicles from human dental pulp mesenchymal stem cells according to claim 1, characterized in that: The steering mechanism comprises a motor (17), a rotating shaft (18), a gear (19) and an annular tooth groove (22); one side of the extraction box (1) is fixedly connected to a side box body (15); the inside of the side box body (15) is fixedly connected to the motor (17); the output end of the motor (17) is fixedly connected to the rotating shaft (18); the other end of the rotating shaft (18) is fixedly connected to the gear (19); an annular tooth groove (22) is provided at the bottom of the centrifugal rotating box body (12); and the gear (19) meshes with the annular tooth groove (22).

7. The device for separating and extracting vesicles from human dental pulp mesenchymal stem cells according to claim 6, characterized in that: The upper and lower sides of the interior of the side box body (15) are fixedly connected to a liquid storage tank (16), and the liquid storage tank (16) is connected to the interior of the funnel (9) through a hose.

8. The device for separating and extracting vesicles from human dental pulp mesenchymal stem cells according to claim 1, characterized in that: The feeding mechanism and the layered extraction drawer (23) are both located on the upper and lower sides of the same side inside the extraction box (1) and can be pulled out.

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