Separation and extraction device for islet cells

Abstract

The utility model discloses a separation and extraction device for islet cells, and relates to the technical field of cell separation and extraction equipment. The device comprises a machine body, a turnover cover plate is arranged at the top of the machine body, a circular groove is formed in the top of the machine body, a first rotatable rotating shaft is movably installed on the inner bottom face of the circular groove, and an annular plate is fixedly arranged at the top end of the first rotating shaft in a sleeved mode. According to the islet cell separation device, the cover plate is overturned to a horizontal state, the machine body is started for cell separation, at the moment, the first rotating shaft drives the annular plate, the test tube groove and a test tube in the test tube groove to rotate, a sample in the test tube groove is gradually separated under the influence of centrifugal force, and after islet cell separation is finished, only the transmission assembly needs to be started; the transmission assembly can drive the four lifting plates to move upwards, when the four lifting plates move upwards, the test tubes in the four test tube grooves can be pushed upwards, so that each test tube is exposed from the interior of the corresponding test tube groove, and the test tubes can be taken conveniently even if the test tubes are short.

Description

Technical Field

[0001] The utility model relates to the technical field of cell separation and extraction equipment, in particular to a separation and extraction device for pancreatic islet cells. Background Art

[0002] Islet cells are a relatively important cell tissue in the human body. They are mainly found in the pancreas and are a hormone component secreted by the pancreas. They can promote the decomposition and utilization of sugar in the body. They can also participate in the decomposition of fat and effectively promote the metabolism of fat in the body. After the ingestion of high-protein food, it can also be gradually decomposed under the action of islet cells. In addition, it also has the functions of promoting the entry of amino acids into cells, promoting blood sugar synthesis, and regulating blood sugar.

[0003] Currently, cell separation technologies include centrifugation technology, flow cytometry and cell electrophoresis. Centrifugation technology is usually used for islet cell separation. The centrifugal force of the cell separator is used to separate the islet cells from the mixed solution. However, when the existing cell separator is used, it is not convenient to take out short test tubes. This is mainly because the test tube slot for placing the test tube is deep. When the short test tube is placed in it, it will be completely placed inside the test tube slot. After the cell separation is completed, it is not convenient to take the test tube out of the test tube slot. Therefore, a separation and extraction device for islet cells is proposed. Utility Model Content

[0004] The purpose of the utility model is to solve the problem that it is inconvenient to take out a short test tube when using an existing cell separator, mainly because the test tube slot for placing the test tube is deep. When the short test tube is placed in the test tube slot, it will be completely placed inside the test tube slot. After the cell separation is completed, it is inconvenient to take the test tube out of the test tube slot. The utility model provides a separation and extraction device for pancreatic islet cells.

[0005] In order to achieve the above-mentioned purpose, the utility model specifically adopts the following technical solutions:

[0006] A device for separating and extracting pancreatic islet cells comprises a body, a reversible cover is arranged on the top of the body, a circular groove is provided on the top of the body, a rotatable rotating shaft 1 is movably installed on the inner bottom surface of the circular groove, a ring plate is fixedly sleeved on the top of the rotating shaft 1, four evenly distributed circular holes are provided on the top of the ring plate, a test tube slot is fixedly installed inside each circular hole, a lifting plate is arranged inside each test tube slot, and a transmission component for driving the four lifting plates to move upward or downward is arranged inside the circular groove.

[0007] Furthermore, the transmission assembly includes strip holes, each test tube slot is provided with a strip hole on the side wall, each lifting plate is fixedly installed with a connecting rod on the side wall, each connecting rod passes through the interior of an adjacent strip hole, a circular ring is sleeved on the outside of the rotating shaft 1, an annular groove is provided on the inner wall of the circular ring, a connecting block is fixedly installed on the outer wall of the circular ring, a rack is fixedly installed on the side of the connecting block away from the circular ring, a servo motor is fixedly installed on the inner wall of the circular groove, the output end of the servo motor is fixedly connected with a rack, and the rack and the connecting block are meshingly connected.

[0008] Furthermore, the inner wall surface of each of the test tube slots is provided with three limit slots, a limit block is movably installed inside each limit slot, and each limit block is fixedly connected to an adjacent lifting plate.

[0009] Furthermore, a fixing assembly for fixing the test tube is arranged on the top of the annular plate, and a groove is provided on the bottom surface of the cover plate in a horizontal state.

[0010] Furthermore, the fixing assembly includes a rotating shaft 2, the top end of the rotating shaft 1 is provided with a rotatable rotating shaft 2 through a damping rotation activity, and the side wall surface of the rotating shaft 2 is fixedly provided with four evenly distributed rotating rods, each rotating rod has a threaded hole on the top, and a bolt is threadedly connected to the inside of each threaded hole.

[0011] Furthermore, the fixing assembly also includes an anti-skid pad, and the bottom end of each bolt is fixedly installed with an anti-skid pad, and the anti-skid pad is made of elastic rubber material.

[0012] The beneficial effects of the utility model are as follows:

[0013] 1. When islet cells are separated, the utility model first places the test tube containing the sample inside the test tube slot, then flips the cover plate to a horizontal state and starts the body to separate the cells. At this time, the rotating shaft 1 will drive the annular plate, the test tube slot and the test tube inside the test tube slot to rotate, and the internal sample of the test tube slot is gradually separated under the influence of centrifugal force. When the islet cell separation is completed, it only needs to start the transmission component, and the transmission component can drive the four lifting plates to move upward. When the four lifting plates move upward, they can push the test tubes inside the four test tube slots upward respectively, so that each test tube is exposed from the inside of the corresponding test tube slot, and it is convenient to take even if the test tube is short. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0015] Figure 2 It is a specific schematic diagram of the test tube tank of the utility model;

[0016] Figure 3 It is a schematic diagram of the partial structure of the transmission component of the utility model;

[0017] Figure 4 This utility model Figure 1 The enlarged view of point A in the middle;

[0018] Figure numerals: 1, body; 2, cover plate; 3, circular groove; 4, rotating shaft one; 5, annular plate; 6, circular hole; 7, test tube groove; 8, lifting plate; 9, transmission assembly; 91, bar hole; 92, connecting rod; 93, circular ring; 94, annular groove; 95, connecting block; 96, rack; 97, gear; 98, servo motor; 10, limit groove; 11, limit block; 12, fixing assembly; 121, rotating shaft two; 122, rotating rod; 123, threaded hole; 124, bolt; 125, anti-slip pad; 13, groove. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0020] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] It should be noted that similar reference numerals and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings. In addition, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0022] In the description of the embodiments of the present invention, it should be noted that the terms "inside", "outside", "upper", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the utility model product is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0023] like Figures 1 to 4As shown, a device for separating and extracting pancreatic islet cells comprises a body 1, a reversible cover plate 2 is arranged on the top of the body 1, a circular groove 3 is provided on the top of the body 1, a rotatable shaft 4 is movably installed on the inner bottom surface of the circular groove 3, a ring plate 5 is fixedly sleeved on the top of the shaft 4, four evenly distributed circular holes 6 are opened on the top of the ring plate 5, a test tube groove 7 is fixedly installed inside each circular hole 6, a lifting plate 8 is arranged inside each test tube groove 7, a transmission assembly 9 for driving the four lifting plates 8 to move upward or downward is arranged inside the circular groove 3, it should be noted that when islet cells are separated, the sample is firstly placed on the plate 100 containing the sample. The test tube is placed inside the test tube slot 7, and then the cover plate 2 is flipped to a horizontal state and the body 1 is started for cell separation. At this time, the rotating shaft 4 will drive the annular plate 5, the test tube slot 7 and the test tube inside the test tube slot 7 to rotate, and the internal sample of the test tube slot 7 is gradually separated under the influence of centrifugal force. When the islet cell separation is completed, it is only necessary to start the transmission component 9, and the transmission component 9 can drive the four lifting plates 8 to move upward. When the four lifting plates 8 move upward, they can push the test tubes inside the four test tube slots 7 upward respectively, so that each test tube is exposed from the inside of the corresponding test tube slot 7, and it is convenient to take out even if the test tube is short.

[0024] like Figures 1 to 3 As shown, the transmission assembly 9 includes a strip hole 91, each test tube slot 7 is provided with a strip hole 91 on the side wall, each lifting plate 8 is fixedly installed with a connecting rod 92 on the side wall, each connecting rod 92 passes through the interior of the adjacent strip hole 91, the outer sleeve of the rotating shaft 4 is provided with a circular ring 93, the inner wall of the circular ring 93 is provided with an annular groove 94, a connecting block 95 is fixedly installed on the outer wall of the circular ring 93, a rack 96 is fixedly installed on the side of the connecting block 95 away from the circular ring 93, and the inner wall of the circular slot 3 is fixedly installed There is a servo motor 98, and the output end of the servo motor 98 is fixedly connected to a rack 96, and the rack 96 is meshingly connected to the connecting block 95. It should be noted that when the servo motor 98 is started, the cover plate 2 can drive the gear 97 to rotate back and forth, and the reciprocating rotation of the gear 97 can drive the rack 96 to move up and down. The up and down movement of the rack 96 will also drive the connecting block 95, the annular groove 94, the connecting rod 92, and the lifting plate 8 to move up and down synchronously. When the lifting plate 8 rotates, it will drive the connecting rod 92 connected to it to slide inside the ring 93.

[0025] like Figure 2As shown, the inner wall surface of each test tube slot 7 is provided with three limit slots 10, and a limit block 11 is movably installed inside each limit slot 10. Each limit block 11 is fixedly connected to the adjacent lifting plate 8. It should be noted that when the lifting plate 8 moves up and down, it will drive the three limit blocks 11 connected to it to slide up and down inside the corresponding limit slots 10 respectively. The limit slots 10 and the limit blocks 11 cooperate with each other to limit the lifting plate 8, so that the lifting plate 8 can move up and down horizontally and stably.

[0026] like Figure 1 As shown, a fixing assembly 12 for fixing the test tube is provided on the top of the annular plate 5, and a groove 13 is provided on the bottom surface of the cover plate 2 in a horizontal state. It should be noted that when the test tube is placed inside the test tube groove 7, the fixing assembly 12 can be rotated to a suitable angle and then pressed down to fix the test tube.

[0027] like Figure 1 , Figure 4 As shown, the fixing assembly 12 includes a second rotating shaft 121, and a rotatable second rotating shaft 121 is installed on the top of the rotating shaft 14 through a damping rotation activity, and four evenly distributed rotating rods 122 are fixedly installed on the side wall of the rotating shaft 121, and a threaded hole 123 is opened on the top of each rotating rod 122, and a bolt 124 is threadedly connected inside each threaded hole 123. It should be noted that by rotating the four rotating rods 122, the four rotating rods 122 are respectively placed directly above the four test tube slots 7, and then each bolt 124 is rotated to move down and press the test tube inside the test tube slot 7. When taking out the test tube, only the four rotating rods 122 need to be rotated to move the four rotating rods 122 away from the top of the four test tube slots 7 respectively.

[0028] like Figure 1 , Figure 4 As shown, the fixing assembly 12 also includes an anti-skid pad 125. The bottom end of each bolt 124 is fixedly installed with an anti-skid pad 125. The anti-skid pad 125 is made of elastic rubber material. It should be noted that the anti-skid pad 125 can increase the friction force and fix the test tube in the event of damage to prevent the test tube from shaking inside the test tube slot 7.

[0029] In summary:

[0030] When islet cells are separated, the test tube containing the sample is first placed inside the test tube slot 7, then the cover plate 2 is flipped to a horizontal state and the body 1 is started for cell separation. At this time, the rotating shaft 4 will drive the annular plate 5, the test tube slot 7 and the test tube inside the test tube slot 7 to rotate, and the internal sample of the test tube slot 7 is gradually separated under the influence of centrifugal force. When the islet cell separation is completed, it is only necessary to start the transmission component 9, and the transmission component 9 can drive the four lifting plates 8 to move upward. When moving upward, the four lifting plates 8 can push the test tubes inside the four test tube slots 7 upward respectively, so that each test tube is exposed from the inside of the corresponding test tube slot 7, and it is convenient to take even if the test tube is short.

[0031] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and the specification only describe the principles of the utility model. The utility model may be subject to various changes and improvements without departing from the spirit and scope of the utility model. These changes and improvements fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A device for separating and extracting pancreatic islet cells, characterized in that: The invention comprises a machine body (1), a reversible cover plate (2) is arranged on the top of the machine body (1), a circular groove (3) is provided on the top of the machine body (1), a rotatable rotating shaft (4) is movably mounted on the inner bottom surface of the circular groove (3), a ring plate (5) is fixedly sleeved on the top of the rotating shaft (4), four evenly distributed circular holes (6) are arranged on the top of the ring plate (5), a test tube groove (7) is fixedly mounted inside each circular hole (6), a lifting plate (8) is arranged inside each test tube groove (7), and a transmission assembly (9) is arranged inside the circular groove (3) for driving the four lifting plates (8) to move upward or downward.

2. A device for islet cell separation and extraction according to claim 1, characterized in that: The transmission assembly (9) comprises a strip hole (91), the side wall surface of each test tube groove (7) is provided with a strip hole (91), the side wall surface of each lifting plate (8) is fixedly mounted with a connecting rod (92), each connecting rod (92) passes through the interior of an adjacent strip hole (91), the outer surface of the rotating shaft (4) is sleeved with a circular ring (93), the inner wall surface of the circular ring (93) is provided with an annular groove (94), a connecting block (95) is fixedly mounted on the outer wall surface of the circular ring (93), a rack (96) is fixedly mounted on the side of the connecting block (95) away from the circular ring (93), a servo motor (98) is fixedly mounted on the inner wall surface of the circular groove (3), the output end of the servo motor (98) is fixedly connected with the rack (96), and the rack (96) and the connecting block (95) are meshingly connected.

3. The device for islet cell separation and extraction according to claim 1, characterized in that: The inner wall surface of each test tube slot (7) is provided with three limit slots (10), a limit block (11) is movably installed inside each limit slot (10), and each limit block (11) is fixedly connected to an adjacent lifting plate (8).

4. The device for islet cell separation and extraction according to claim 1, characterized in that: A fixing assembly (12) for fixing the test tube is arranged on the top of the annular plate (5), and a groove (13) is provided on the bottom surface of the cover plate (2) in a horizontal state.

5. A device for islet cell separation and extraction according to claim 4, characterized in that: The fixing assembly (12) comprises a second rotating shaft (121), the top end of the first rotating shaft (4) is provided with a rotatable second rotating shaft (121) through a damping rotation movement, and the side wall surface of the second rotating shaft (121) is fixedly provided with four evenly distributed rotating rods (122), the top of each rotating rod (122) is provided with a threaded hole (123), and the interior of each threaded hole (123) is threadedly connected with a bolt (124).

6. The device for islet cell separation and extraction according to claim 5, characterized in that: The fixing assembly (12) further comprises an anti-skid pad (125), and the bottom end of each bolt (124) is fixedly mounted with an anti-skid pad (125), and the anti-skid pad (125) is made of elastic rubber material.

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