Monitoring and control system for double-stacked three-dimensional water-based microfluidic culture ecological container

Abstract

The utility model discloses a monitoring and control system for a double-stacked sanitary type three-dimensional water-based microfluidic culture ecological container. The monitoring and control system comprises an ecological container monitoring and control unit, a groove type functional plug-in framework, a sanitary type mounting rack, an image acquisition system, a data communication module and the like, the modular design is adopted, and the ecological container unit, the temperature control unit, the monitoring unit and other functional boards can be combined as required to form a complete test or production chain. All double-stacked three-dimensional water-based microfluidic ecological container monitoring and control units are integrated into a whole, a production process chain formed by a plurality of culture units is realized, N + 1 sensing systems are deployed, and a big data monitoring network is formed.

Description

Technical Field

[0001] The utility model relates to the technical field of plant tissue culture, in particular to a double-stacked guard type three-dimensional water-based microfluidic culture ecological container monitoring and control system. Background Art

[0002] The development of water-based cell and tissue culture technology has not only promoted the research of subjects such as botany, but also provided strong support for the innovation and application of biological cultivation technology. Water-based cell and tissue culture technology is used to cultivate plants under soilless or water-based conditions. The development of water-based cell and tissue culture technology has promoted the research of subjects such as botany, provided strong support for biological cultivation, and better understood the growth, development and metabolic processes of plants by controlling the nutrients in the culture medium.

[0003] In modern biological research, high-resolution microscopy is used to observe plant cell structure, division, and physiological characteristics. Gene editing technology is used to precisely modify genes and study the effects on plant growth and development. Combining bioinformatics and big data analysis technology, plant gene expression, metabolic pathways, and biological networks can be studied at the whole genome level. Sensors and intelligent monitoring technologies are developed to monitor environmental conditions in plant water-based culture systems in real time to optimize growth conditions.

[0004] At present, the known automatic water-based cultivation system is mostly composed of an industrialized assembly line, a nutrient fertilizer storage tank and multiple sets of sensors, and still requires manual operation. There are many existing intelligent plant cultivation equipment products, but they are mainly used for batch cultivation. They cannot be applied in finely controlled experimental environments and transgenic plant experiments. The same set of equipment configuration standards are used for different tasks and scenarios, which cannot meet the needs of the experiment and require additional labor costs. For different plant species and experimental purposes, it is necessary to optimize the composition and ratio of the culture medium, and provide suitable nutrients and growth conditions through fine control. The automation degree of the plant water-based cultivation system is not high, and the function is limited. In actual research work, it is necessary not only to be able to monitor and adjust the cultivation conditions in real time, but also to provide flipping and appropriate oscillation operations. Microbial contamination is prone to occur in water-based cultivation. It is difficult for existing equipment to effectively control the growth of microorganisms in water-based cultivation systems, and water quality is difficult to guarantee. Existing equipment cannot be used in parallel or in series, few provide digital analysis technology, and no accurate fluid monitoring and control system is set, and the liquid flow and oscillation frequency in the culture container cannot be controlled.

[0005] The known plant cultivation equipment fails to realize the rotation and movement of the plant cultivation container in space, and is limited to fixed installation, rather than automatically adjusting the gravity distribution state of the plant as needed. The existing hydroponic system does not have a micro-photography system, and cannot realize real-time monitoring of the microscopic environment of plant growth, and is not suitable for research on plant growth and development. The functions used are single, and the filtering and temperature control devices cannot be quickly connected. The low operating efficiency is also not conducive to the technical regulation and light assembly requirements in the production environment. The known intelligent plant cultivation equipment basically belongs to environmental control equipment, which observes the growth of plants through sensors and ordinary cameras, and has not realized the remote automation and digitization of plant research.

[0006] Foreign automatic water-based cultivation systems are equipped with automatic cultivation and micro-camera systems, which are suitable for plant growth and development research and can observe the microscopic growth of plants. However, the equipment is large in size and expensive, and the overall structure is not flexible. Utility Model Content

[0007] In view of the shortcomings of the existing technology, the utility model provides a double-stacked three-dimensional water-based microfluidic culture ecological container monitoring and control unit, which can be combined with various types of cultivation container parts, temperature control panels, medicine storage containers and monitoring sensor panels, and use wireless communication systems such as Bluetooth to upload to multiple groups of available servers.

[0008] To achieve the above purpose, the utility model is implemented through the following technical solutions: a double-stacked three-dimensional water-based microfluidic culture ecological container monitoring and control system, characterized in that it includes a server, a switch, a control terminal for controlling the operating status of each unit in the system, a microcomputer connected to the control terminal and transmitting control information to each connected unit, a functional solution storage container, and an ecological container monitoring control unit;

[0009] The microcomputer device is installed around the double-stacked three-dimensional water-based microfluidic ecological container monitoring control unit and connected through a signal line or a wireless network; a microcomputer device can control the surrounding units that have formed a local area network; the microcomputer device communicates with the server to send and receive data through a wired network or a wireless network;

[0010] Among them, the functional solution storage container is used to store different solutions for water-based culture and to supplement the solutions required for water-based culture;

[0011] The ecological container monitoring control unit includes:

[0012] Slot-type functional plug-in framework: It has multiple slots for inserting cultivation boards with different functions according to needs;

[0013] Cultivation container: inserted into the slot-type functional plug-in frame, used for water-based cultivation, with catheter interfaces on both sides for connecting to the liquid medicine storage container with a catheter, and the catheter interface is provided with a flow control component for controlling fluid input and output, and the peristaltic pump between the catheter interface and the liquid medicine storage container is connected to the control terminal through a data communication module; different areas in the cultivation container are provided with guide valves in each area, which can directly reduce, turn or close the fluid in the area in the container; a turbulence control valve is also provided in the cultivation container, through which the input fluid is dispersed, and the turbulence control valve is of two types: leaf-shaped and comb-shaped, and the fan blades of the turbulence control valve are used as fluid stirring rotating blades; a sensor is also installed, through which the environmental information of the cultivation container is transmitted to the control terminal;

[0014] Health-type mounting frame: It includes an image data acquisition frame, which is equipped with an image acquisition system, whose moving position is controlled by a stepper motor, and is also equipped with a light source for providing illumination for the cultivation container.

[0015] As a further solution of the utility model, the data communication module has WiFi and Bluetooth communication, and has an infrared signal receiving function and an RJ45 network interface;

[0016] As a further solution of the utility model, the culture container has a rectangular cube structure on the outside and a non-rectangular cube space on the inside, in which a trapezoidal structure with a certain slope is arranged. The neck of the lower catheter interface of the culture container is 1.2 to 1.5 times larger than that of the upper catheter interface, and can be connected to a reflux catheter.

[0017] As a further solution of the utility model, the outer frame of the trough-type functional plug-in frame is equipped with a stepping motor, which can raise and lower the upper part of the trough-type functional plug-in frame vertically to the ground. The outer frame is equipped with a rotating motor, and the rotating nodes in the middle of the left and right sides can adjust the horizontal plane of the cultivation container.

[0018] As a further solution of the utility model, the groove-type functional plug-in frame is also inserted with a water flow temperature control plate or a filter plate, which is connected to the culture container through a conduit or a heat-conducting plug-in plate.

[0019] As a further solution of the present invention, the functional solution storage container includes a liquid culture medium storage container, a drug solution storage container, and a circulating liquid buffer storage container.

[0020] As a further solution of the utility model, the ecological cultivation container is embedded with a microfluidic plate, which is connected via a catheter. When the fluidic accuracy of the input catheter is insufficient, the embedded microfluidic plate is used. The microfluidic plate on the cultivation container is connected to the cultivation container via a catheter, so that test substances can be introduced or whether required metabolites are produced can be detected.

[0021] As a further solution of the utility model, the power supply and lines are pre-buried in the ground, waterproof, fireproof and insulated, and a fixing hole is reserved under the ground of each culture ecological container monitoring control unit, and a convex floor mat clip is used to fix the groove-type functional plug-in frame.

[0022] As a further solution of the utility model, an embedded fixing frame is installed on the top of the cultivation container when cultivating plants. The embedded fixing frame is also provided with a catheter interface through hole and a planting hole for embedding the catheter interface. The plants are connected to the cultivation container through the planting hole above the embedded fixing frame.

[0023] The utility model has the following beneficial effects:

[0024] 1. The technical innovation of this utility model lies in the application of integrated production chain technology to combine various biological cultivation, temperature control, and nutrient composition control panels with different functions to form a production chain of an overall system, and digitize plant research by microscopic image scanning and analysis. In a biological experiment and production area, unified control management and data collection of ecological cultivation containers are achieved through catheters and communication cables. All double-stacked three-dimensional water-based microfluidic cultivation ecological container monitoring and control units can be integrated into a whole, realizing a virtual factory composed of multiple production chains, deploying N+1 sensor systems, and forming a big data area.

[0025] 2. Highly integrated hardware configuration combines the environmental control functions of several unconnected areas into a whole, so that the use of one set of culture medium storage containers or the same set of temperature control and filtration devices can meet the needs of cultivation containers of different species or genetically modified plants in the same production chain. Microscopic monitoring with different grid scales can be connected to 3 functional cameras and 1 microscope camera on the image data acquisition frame, and additional shadowless LED light source components and color sensors can be installed to accurately locate the observation point or analyze growth differences with high resolution.

[0026] 3. The double-stacked guard-type three-dimensional water-based microfluidic culture ecological container monitoring and control unit is made into a box-shaped rectangular format shape, with three sizes, 3 fluid input interfaces and 2 fluid input and output interfaces, installed on a guard-type mounting frame, and can be fixed on the ground; it can be applied to various plant growth and development research, plant root development research, plant tissue cultivation, tissue cell cultivation, bacterial species identification, etc., and the monitoring and control unit can be assembled and combined according to needs to have GPS, microscope camera, temperature control, microbial contamination control and other functions to suit various tasks and scenarios.

[0027] 4. The double-stacked three-dimensional water-based microfluidic culture ecological container monitoring and control unit selects appropriate functional panels according to work requirements and scene needs, uses temperature control panels and fluid control panels to supply various nutrients and test solutions required for plant growth, and is used for automated cultivation and observation of plant tissues, complete plants or cells.

[0028] 5. Apply microfluidic technology to the same production area or the same type of culture tissue sample area, apply image acquisition, sensor data acquisition, and microfluidic testing technology to obtain biological data, connect with the server through the communication system, and realize the collection of ecological data and cultivation environment feedback. All double-stacked three-dimensional water-based microfluidic culture ecological container monitoring and control units can be integrated into a whole, and N+1 sensor systems can be deployed to realize a production area composed of multiple units.

[0029] 6. The double-stacked three-dimensional water-based microfluidic culture ecological container monitoring and control unit has the function of applying the default settings or normally open settings when the equipment is disconnected. In the case of network communication interruption or server response failure, the microcomputer automatically controls the function board to execute various ecological maintenance instructions according to the preset parameters; it has threshold warning and automatic shutdown functions. It has the functions of toxic and harmful substance detection and limited protein detection and separation.

[0030] 7. The double-stacked three-dimensional water-based microfluidic culture ecological container monitoring and control unit adopts modular assembly technology in both functions and functional components. Microscopic cameras or microscopic cameras can be configured and used as needed to greatly improve resource utilization and reduce investment costs. A functional board such as a filter board and a temperature control board can be shared to increase the equipment usage time and consumable board utilization. The sharing of filter boards must comply with design specifications. The temperature control board itself uses water circulation heat exchange and is not directly connected to the ecological container. The circulating hot water can be modulated as needed.

[0031] 8. The double-stacked three-dimensional water-based microfluidic culture ecological container monitoring and control unit can rotate the culture container plane to achieve vertical, parallel, and inclined horizontal plane deployment, and provide oscillation functions according to different needs; it can realize the timed oscillation and periodic oscillation of the culture container. It is also possible to insert an oscillation function board to directly oscillate the culture ecological container. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a schematic diagram of the system structure of the utility model;

[0033] Figure 2 This is a schematic diagram of an ecological container monitoring control unit of the utility model;

[0034] Figure 3 This is a schematic diagram of the structure of the cultivation container of the utility model;

[0035] Figure 4 This is a schematic diagram of the relationship between the embedded fixing frame and the cultivation container of the utility model;

[0036] Figure 5 This is a schematic diagram of the relationship between the trapezoidal structure and the cultivation container of the utility model;

[0037] Figure 6 It is a front view and a side view of the trapezoidal structure of the cultivation container of the utility model;

[0038] Figure 7 This is a schematic diagram of the turbulence control valve of the utility model.

[0039] In the figure: 1. server; 2. switch; 3. control terminal; 4. microcomputer; 5. functional solution storage container; 6. ecological container monitoring control unit; 41. slot-type functional plug-in frame; 42. sanitary mounting frame; 421. stepper motor; 422. image acquisition system; 43. cultivation container; 44. catheter interface; 45. trapezoidal structure; 46. fluid steering control valve; 47. turbulence valve; 48. embedded fixing frame; 49. planting hole; 50. catheter interface through hole. DETAILED DESCRIPTION

[0040] The utility model provides the following, in conjunction with the drawings in the embodiments of the utility model, to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the utility model. The utility model belongs to a highly technical equipment system for plant cultivation, which is designed for three-dimensional plant cultivation and uses liquid culture medium-based microfluidic technology. Microfluidic technology and turbulence control technology are used to accurately control minerals, organic matter, water and other growth factors, and plant growth and development are automatically regulated at set time points through plant growth interferon, gibberellin, auxin, etc. Turbulence technology is used to increase the oxygen transmission rate in the culture solution, increase the growth rate of cells or microorganisms, and improve the proliferation efficiency of organisms that are cultured at high density or require high metabolic activity. Mild turbulence promotes cell stress, causing it to produce more secondary metabolites. Turbulence technology evenly distributes the products in the culture solution, making the collection of products more efficient. Promote local flow in the culture medium, ensure uniform mixing of culture materials, and effectively remove waste products.

[0041] The Eco-Container Foundation has a two-layer structure that can be deployed in a plug-in manner, with two vertically stacked sections or layers that contain the elements required for the entire ecosystem, such as soil, water and nutrients. It has units responsible for monitoring and controlling various aspects of the system. It applies remote sensors to monitor the environmental conditions of plant growth, as well as units that control elements such as water flow, nutrient supply, etc.

[0042] With modular design, functional boards such as ecological container units, temperature control units and monitoring units can be combined as needed to form a complete test or production chain. All double-stacked three-dimensional water-based microfluidic ecological container monitoring and control units 6 are integrated into a whole to realize a production process chain composed of multiple culture units, and N+1 sensor systems are deployed to form a big data monitoring network.

[0043] For details, please refer to Figure 1 -7, a double-stacked three-dimensional water-based microfluidic culture ecological container monitoring and control system of this embodiment, characterized by comprising a server 1, a switch 2, a control terminal 3 for controlling the operating status of each unit in the system, a microcomputer 4 connected to the control terminal 3 and transmitting control information to each connected unit, a functional solution storage container 5, and a plurality of ecological container monitoring control units 6;

[0044] Among them, the functional solution storage container 5 is used to store different solutions for water-based culture, and is used to supplement the solutions required for water-based culture;

[0045] The ecological container monitoring control unit 6 includes:

[0046] Slot-type functional plug-in frame 41: having multiple slots for inserting cultivation boards with different functions according to needs;

[0047] Cultivation container 43: inserted into the slot-type functional plug-in frame 41, used for water-based cultivation, the cultivation container 43 has 3 catheter interfaces 44 on the upper part and 2 catheter interfaces 44 on the lower part, which are used to connect with the liquid medicine storage container with a catheter, and the catheter interface 44 is provided with a flow control component, specifically, the flow control interface or the flow control valve is installed between the connecting catheters to achieve stable fluid input and output, the connecting catheter controls the fluid to enter the cultivation container 43 according to the thickness of the tube neck, and cooperates with the peristaltic pump; in the scenario where the demand is not high, the liquid pump can be directly installed at the outlet of the storage container, and the fluid can be introduced by controlling the pressure and the catheter. The peristaltic pump between the catheter interface 44 and the liquid medicine storage container is connected to the control terminal 3 through the data communication module, and runs offline when disconnected from the control terminal 3, and the default frequency can be set to keep the equipment working stably; different areas in the cultivation container 43 are provided with guide valves in each area, which can directly reduce, turn or close the fluid in the area in the container; the cultivation container 43 is deployed with a guide valve, which can directly reduce, turn or close the fluid in the area in the container under some specific needs. A turbulence control valve is deployed in the cultivation container 43, which is similar to the interface in the conduit, and the input fluid is dispersed through the turbulence control valve; a sensor is also installed, and the environmental information of the cultivation container 43 is transmitted to the control terminal 3 through the sensor;

[0048] Furthermore, the cultivation container 43 is made of PVC material, and the internal channel design can be used to select the cultivation container 43 for use in separated blocks or without separated blocks. When used in separated blocks, the turbulence control valves between the blocks specifically include a fluid steering control valve 46 and a turbulence valve 47, and the turbulence valve 47 is of two types: leaf-shaped and comb-shaped. In some special cases, the fan blades of the turbulence valve 47 can be used as fluid stirring rotating blades, and the fluid steering control valve 46 can be omitted when used without separated blocks. The fluid flow control valve can be installed at the catheter interface 44, and the fluid steering control valve 46 can be installed at the catheter branch.

[0049] The catheter interface 44 uses a stretch interface, which is disinfected and inserted with a sealing ring before connection. The entire system should be disinfected before operation, and the disinfectant is connected to clean the entire system. After cleaning, the disinfectant container is removed and connected to the buffer flushing liquid. After the entire system is disinfected and cleaned, it is connected to the liquid culture medium storage container. Disinfection and cleaning are convenient and do not need to be disassembled.

[0050] The sanitary mounting frame 42 includes an image data acquisition frame, an image acquisition system 422 is installed, and a light source for providing light to the culture container 43 is installed. Figure 7 , controlling the moving position of the image acquisition system 422 through the stepping motor 421;

[0051] The image acquisition system is specifically a microscopic camera system, which collects the growth and development of plants or cells in the cultivation container 43; determines the area that needs to be scanned carefully through the visual sensor, and then uses the microscopic camera to locate the shooting area. The collected image data is stored by the microcomputer 4 and sent to the server 1.

[0052] The ecological container monitoring control unit and the ecological monitoring sensor panel can be combined through the conduit and the signal connection line, and the complete production chain can be realized through function selection and server 1 control setting.

[0053] For further information, see Figure 4 When cultivating plants, an embedding fixing frame 48 is installed on the upper part of the cultivation container 43. The embedding fixing frame 48 is also provided with a catheter interface through hole 50 for embedding the catheter interface 44 and a planting hole 49. The plant is connected to the cultivation container 43 through the planting hole 49 on the embedding fixing frame 48. The cultivation container 43 uses different embedding fixing frames 48 as needed. The embedding fixing frame 48 is still configured with three catheter interfaces 44, but with one more planting hole 49. A maximum of two planting holes 49 can be provided.

[0054] The data communication module has wifi and bluetooth communication, and has infrared signal receiving function and RJ45 network interface;

[0055] The ecological container monitoring control unit 6 uses a temperature control panel with a double-layer structure, one layer for hot liquid circulation and one layer for cold liquid circulation. There is a temperature insulation layer between the conduits, which will not affect the performance of other conduits or functional panels. The hot and cold cycles are controlled by the flow of the introduced liquid, and the temperature is accurately identified by the deployed temperature sensor.

[0056] The state container monitoring control unit 6 uses a filtering function board. The filtering function board uses an S-type deployment or I-type deployment filter strip filter, which can be sterilized and replaced.

[0057] Preferably, the ecological cultivation container 43 is divided into two types, with scale and without scale, and a scale transparent plate is installed or added as needed, and inserted into the slot-type functional plug-in frame 41. Various functional plug-ins are inserted into the slot-type functional plug-in frame 41 as needed, interconnected through conduits and connection interfaces, and connected to the microcomputer 4 through network signal lines. The microcomputer 4 communicates with the server 1 through network equipment interconnection.

[0058] Furthermore, all functional panels, frames, and containers are made of highly transparent PVC. When necessary, a sunshade or spectrum filter is inserted into the frame to reduce light or illumination. The cultivation container 43 deployed on the horizontal plane can be placed in a semi-open position, but there are special requirements for environmental disinfection and nutrient solution.

[0059] A sanitary mounting frame 42 with a stepper motor and a rotary motor is configured, and the cultivation container 43 is rotated or flipped by the microcomputer 4 to meet the needs of the production chain operation; according to the cultivation needs, functional components such as plant growth light sources, specific spectrum light sources, and shadowless lighting light sources can be configured and installed on the top horizontal frame of the sanitary mounting frame 42; all functional panel interfaces and data communication interfaces are compatible with each other. Deployment is carried out using the insertion installation method.

[0060] A plant growth lamp and a shadowless LED light source are installed on the upper horizontal frame of the sanitary mounting frame 42. Sensors can be installed at specific positions on the surface of the functional panel as needed, and temperature control sensors, PH sensors, oxygen sensors, etc. can be installed between the connecting ducts.

[0061] After receiving the instruction from the server, the IoT communication module manages and controls the functional components such as the motor, camera and sensor through the control module.

[0062] The slot-type functional plug-in frame 41 has 3 to 5 slots for inserting up to 2 card-type ecological cultivation containers 43, which are divided into cultivation containers 43 of different plane area sizes according to the characteristics of the cultivated plants, and the frame is adjustable. The outer frame is equipped with a stepper motor, which can lift and lower the upper half of the slot-type functional plug-in frame 41 vertically to the ground. The stepper motors on the left and right sides adjust the height of the cultivation container 43 by adjusting the height of the vertical telescopic frame to adapt to the stacked deployment of the cultivation container, reduce space loss, and increase the utilization rate of light. The outer frame is equipped with a rotating motor, and the rotating nodes in the middle of the left and right sides can adjust the horizontal plane of the cultivation container 43. When using a vibration function panel, a rectangular soft rubber inner frame must be embedded in the slot-type functional plug-in frame 41. The slot-type functional plug-in frame 41 can be inserted with a water flow temperature control plate or a filter plate as needed, and connected to the cultivation container 43 through a conduit or a heat-conducting plug-in plate.

[0063] There are three sizes of the slot-type functional plug-in frame 41, which are suitable for different application scenarios; based on the cultivation container 43, three cultivation containers 43 can be inserted at a time, and the containers are connected in parallel or in series with catheters; specifically: three catheter interfaces 44 are provided on the upper part of the controlled cultivation container 43, which are connected with PVC high-permeability culture containers, PVC high-permeability storage containers for medicinal liquids, liquid culture medium storage containers, medicinal liquid storage containers, circulating liquid buffer storage containers, etc. through catheters to achieve management and control of ecological environment maintenance; it can also be used in series or parallel with other functional panels through catheters or connecting components.

[0064] Furthermore, the system as a whole has pressure control and gas circulation control. The gas circulation uses two methods: direct introduction and introduction of fluid rich in gas elements. The pressure control is controlled by a buffer storage container and a gas filter. The gas filter control is directly connected to the liquid culture medium container. Figure 5The culture container 43 has a rectangular cube structure on the outside and a non-rectangular cube space on the inside. There is a trapezoidal structure 45 with a specific slope inside. The necks of the two catheter interfaces 44 at the bottom of the culture container 43 are 1.2 to 1.5 times larger than the three catheter interfaces 44 at the top, and can be connected to the reflux catheter. The fluid injected into the reflux catheter slows down the reflux speed and impact force through the trapezoidal structures 45 and the turbulence control knob, and the reflux fluid is more evenly dispersed.

[0065] The ecological cultivation container 43 has a built-in microfluidic plate installed in a groove at a dedicated position of the cultivation container 43 and connected via a catheter. The built-in microfluidic plate is used when the input catheter fluidic accuracy is insufficient, and provides injection and detection functions.

[0066] The microfluidic plate interface on the culture container 43 uses a universal size and is connected to the culture container 43 through a catheter to introduce test substances or detect whether required metabolites are produced. It supports electronic microfluidic plate access, and the interface communication line is connected to the communication interface of the microcomputer 4.

[0067] The power supply and circuits of the utility model are pre-buried in the ground, and are waterproof, fireproof, and insulated. A fixing hole is reserved under the ground for each ecological container monitoring control unit 6, and a convex floor mat clip can be used to fix the slot-type functional plug-in frame 41.

[0068] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0069] In the description of the present invention, it should be understood that terms such as "opening", "upper", "lower", "thickness", "top", "middle", "length", "inside", "all around" and the like indicating orientation or positional relationship are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0070] The preferred embodiments of the utility model disclosed above are only used to help explain the utility model. The preferred embodiments do not describe all the details in detail, nor do they limit the utility model to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that technicians in the relevant technical field can well understand and use the utility model. The utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A double-stacked three-dimensional water-based microfluidic culture ecological container monitoring and control system, characterized in that: The system comprises a server, a switch, a control terminal for controlling the operating status of each unit in the system, a microcomputer connected to the control terminal and transmitting control information to each connected unit, a functional solution storage container, and a plurality of ecological container monitoring control units; the microcomputer device is installed around the double-stacked three-dimensional water-based microfluidic ecological container monitoring control unit and connected through a signal line or a wireless network; a microcomputer device can control the surrounding units that have formed a local area network; the microcomputer device communicates with the server to send and receive data through a wired network or a wireless network; Among them, the functional solution storage container is used to store different solutions for water-based culture and to supplement the solutions required for water-based culture; The ecological container monitoring control unit includes: Slot-type functional plug-in frame: It has multiple slots for inserting cultivation boards with different functions; Cultivation container: inserted into the slot-type functional plug-in frame, used for water-based cultivation, with catheter interfaces on both sides for connecting to the liquid medicine storage container with a catheter, and the catheter interface is provided with a flow control component for controlling fluid input and output, and the peristaltic pump between the catheter interface and the liquid medicine storage container is connected to the control terminal through a data communication module; different areas in the cultivation container are provided with guide valves in each area, which can directly reduce, turn or close the fluid in the area in the container; a turbulence control valve is also provided in the cultivation container, and the input fluid is dispersed through the turbulence control valve; a sensor component is also installed, and the environmental information of the cultivation container is transmitted to the control terminal through the sensor component; Health-type mounting frame: It includes an image data acquisition frame, which is equipped with an image acquisition system, whose moving position is controlled by a stepper motor, and is also equipped with a light source for providing illumination for the cultivation container.

2. The monitoring and control system according to claim 1, characterized in that: When cultivating plants, an embedding fixing frame is installed on the top of the cultivation container. The embedding fixing frame is also provided with a catheter interface through hole for embedding the catheter interface and a planting hole. The plant is connected to the cultivation container through the planting hole above the embedding fixing frame.

3. The monitoring and control system according to claim 1, characterized in that: The data communication module has WiFi and Bluetooth communications, and has an infrared signal receiving function and an RJ45 network interface.

4. The monitoring and control system according to claim 1, characterized in that: The culture container has a rectangular cube structure on the outside and a non-rectangular cube space on the inside. A trapezoidal structure with a certain slope is arranged inside the culture container. The neck of the lower catheter interface of the culture container is 1.2 to 1.5 times larger than that of the upper catheter interface, and can be connected to a reflux catheter.

5. The monitoring and control system according to claim 1, characterized in that: The outer frame of the trough-type functional plug-in frame is equipped with a stepping motor, which can lift and lower the upper part of the trough-type functional plug-in frame vertically to the ground. The outer frame is equipped with a rotating motor, and the rotating nodes in the middle of the left and right sides can adjust the horizontal plane of the cultivation container.

6. The monitoring and control system according to claim 1, characterized in that: The slot-type functional plug-in frame is also inserted with a water flow temperature control plate or a filter plate, and is connected to the culture container through a conduit or a heat-conducting plug-in plate.

7. The monitoring and control system according to claim 1, characterized in that: The functional solution storage container includes a liquid culture medium storage container, a drug solution storage container, and a circulating liquid buffer storage container.

8. The monitoring and control system according to claim 1, characterized in that: The culture container is embedded with a microfluidic plate, which is connected to the culture container via a catheter, and can introduce test substances or detect whether required metabolites are produced.

9. The monitoring and control system according to claim 1, characterized in that: The power supply and lines are pre-buried in the ground, with waterproof, fireproof and insulating packages. A fixing hole is reserved under the ground for each culture ecological container monitoring control unit, and a convex floor mat clip is used to fix the slot-type functional plug-in frame.

10. The monitoring and control system according to claim 1, characterized in that: The turbulence control valve specifically includes a fluid steering control valve and a turbulence valve. The turbulence valve is leaf-shaped or comb-shaped, and the fan blades of the turbulence valve serve as fluid stirring rotating blades.

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