Container and device for microalgae density detection

Abstract

The utility model relates to the technical field of optical detection, and discloses a container and a device for microalgae density detection, the container is a step-shaped container, the top view of the container is a circle with different diameters, the device comprises the container, a power supply, a luminous light source, a photoelectric detector and a signal processor, on one hand, it is ensured that incident light can be collimated and incident under a certain condition, and the requirement for reliability is met; on one hand, it is guaranteed that the distance of incident light penetrating through the microalgae solution can be adjusted according to the proportion, the requirement for adjustability is met, analysis and calculation of microalgae solution density testing results under different optical path conditions in the testing process can be achieved, error analysis results are obtained, and testing conditions can be optimized and changed on the basis.

Description

Technical Field

[0001] The utility model relates to the technical field of optical detection, in particular to a container and a device for detecting the density of microalgae. Background Art

[0002] In the field of microalgae cultivation, if the content of microalgae particles needs to be accurately and quickly detected, the current traditional detection methods include dry weight method, ultrasonic detection method, counting method and electrode method. The dry weight method is to centrifuge a certain volume of suspension on a high-speed centrifuge for solid-liquid separation, wash the separated precipitate several times, centrifuge it again, and then dry the particles after centrifugation at high temperature, put it into a constant weight and dry weighing bottle, weigh the mass of the separated particles, and then calculate the content of the particles. The counting method is often used to detect the number of cells, using a cell counter or staining with methylene blue. This method requires observation and counting with a microscope, and requires multiple dilutions, which easily lead to large errors. The working principle of ultrasonic method for detecting concentration is that when ultrasonic waves pass through the suspension, the particles absorb the sound waves, the ultrasonic energy is attenuated, the amplitude is reduced, and the sound intensity, sound velocity or acoustic resistance before and after the ultrasonic wave is incident changes. There is a certain specific relationship between the concentration and the change of ultrasonic waves. According to this principle, the density of suspended particles can be measured. This type of instrument is susceptible to changes in fermentation broth composition, bubbles, temperature changes and viscosity. The electrode method uses a standard electrochemical detection method to measure the concentration of particles in the suspension by using the change in the capacitance between electrodes. However, this method is often used to detect the number of living cells and has limitations for the detection of all granular suspended microalgae.

[0003] With the rapid development of optoelectronic technology, the use of photoelectric methods to detect the concentration of suspensions has attracted the attention of many scholars, and its theory and experiments have been deeply studied. In the development of optoelectronic technology, advanced light sources with good performance continue to emerge, such as light-emitting diodes, infrared light-emitting diodes, lasers, infrared lasers, etc. These light sources have stable working conditions and long life. They can provide stable incident light for a long time during concentration detection, which greatly facilitates the detection of optical signals and improves the accuracy of detection. In addition, the quantum efficiency, light amplification effect, and thermoelectric stability of photodetectors have been greatly improved, which provides guarantees for the accuracy and applicable scenarios of photoelectric detection technology.

[0004] At present, the microalgae density detection technology based on photoelectric technology includes photometer detection, fiber optic detection and other methods. In order to increase the detection accuracy of microalgae density during the detection process, light incident at different angles can be used, and the detection results can be analyzed to calculate the calculation error of the microalgae density. However, in the prior art, circular cylindrical containers or square cylindrical containers are often used to hold microalgae solutions. During the test process, the distance that light passes through the microalgae solution is fixed and does not play a role in regulating the light transmission distance of the microalgae solution. However, the light transmission distance of the microalgae solution determines the degree of change in the attenuation of light intensity, which has a key impact on the test results of the microalgae density. Regulating the light transmission distance of the microalgae solution can achieve the effect of multiple calculations and analysis errors. At present, the relevant technology of microalgae density detection does not involve a container and related devices for quantitatively adjusting the light transmission distance of the microalgae solution.

[0005] The prior art discloses a photobioreactor device, and in particular, relates to a photobioreactor device for high-density cultivation of microalgae, characterized in that: it comprises a photobioreactor with a light source emitter, a reactor discharge port and an air inlet are arranged at the bottom of the photobioreactor, a feed port and an exhaust port are arranged at the top, the air inlet is sequentially connected with an inlet CO2 gas detection device, a gas mixing device and a gas storage tank, the feed port is connected with a peristaltic pump, the exhaust port CO2 gas detection device is connected with the exhaust port, and the exhaust port gas dryer is located between the exhaust port CO2 gas detection device and the exhaust port; the invention has a unique structure, is easy to maintain, can efficiently utilize light energy and fix CO2 gas, and solves the problems of insufficient light energy supply and low CO2 gas utilization rate in high-density cultivation of microalgae in a closed photobioreactor. The prior art has the problem that the light transmission distance of the microalgae solution cannot be quantitatively adjusted, and the density detection accuracy is not high. Utility Model Content

[0006] The utility model aims to provide a container and a device for detecting the density of microalgae, so as to achieve proportional adjustment of the distance that the incident light passes through the microalgae solution, and to test the density of the microalgae solution under different light path conditions.

[0007] In order to achieve the above-mentioned purpose, the utility model provides a container for detecting the density of microalgae, wherein the container is a stepped container, and the top view of the container is a circle with different diameters.

[0008] Preferably, a small opening is provided on the top of the container.

[0009] Preferably, the small opening is provided with a spiral cover.

[0010] Preferably, the container is made of transparent acrylic plate, glass or quartz stone.

[0011] The utility model also provides a device for detecting the density of microalgae, comprising a container, a power supply, a light source, a photodetector and a signal processor based on the above, wherein the container is located between the light source and the photodetector, the light source is opposite to the receiving surface of the photodetector, the power supply is electrically connected to the light source and the photodetector, the light source is communicatively connected to the photodetector, and the photodetector is communicatively connected to the signal processor.

[0012] Preferably, it also includes a container holder, which cooperates with the container surface to maintain the balance of the container during testing at different angles.

[0013] Preferably, it further comprises a lifting mechanism, and the lifting mechanism is used to adjust the height position of the container.

[0014] Preferably, the light source is a laser, a light emitting diode (LED), a micro-LED or a mini-LED.

[0015] Preferably, the photodetector is a PIN diode or an APD diode.

[0016] Preferably, the power supply device is a steady current source or a steady voltage source, and the signal processor is a mobile terminal.

[0017] Compared with the prior art, the beneficial effects of the utility model are:

[0018] A stepped circular container is selected, which, on the one hand, ensures that the incident light can be collimated under certain conditions, meeting the reliability requirement; on the other hand, ensures that the distance the incident light passes through the microalgae solution can be adjusted proportionally, meeting the adjustability requirement. It can be realized that the density test results of the microalgae solution under different optical path conditions during the test process can be analyzed and calculated to obtain error analysis results, and the test conditions can also be optimized and changed on this basis. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a side schematic diagram of a container of an embodiment of the utility model;

[0020] Figure 2 It is a schematic diagram of a device for detecting the density of microalgae when incident light passes through the top of a stepped circular container according to an embodiment of the utility model;

[0021] Figure 3 It is a schematic diagram of a device for detecting the density of microalgae when incident light passes through the side of a stepped circular container according to an embodiment of the utility model;

[0022] Figure 4 It is a schematic diagram of a device for detecting the density of microalgae when incident light passes through the bottom of a stepped circular container according to an embodiment of the utility model.

[0023] In the figure, 1. container; 2. power supply; 3. light source; 4. photoelectric detector; 5. lifting mechanism; 6. container holder; 7. signal processor. DETAILED DESCRIPTION

[0024] The following is a further detailed description of the specific implementation of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0025] In the description of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0026] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0027] In addition, in the description of the present invention, unless otherwise specified, “plurality” means two or more.

[0028] Embodiment 1

[0029] like Figure 1 As shown, a container for detecting microalgae density according to a preferred embodiment of the utility model, the container 1 is a stepped container, and the top view of the container 1 is a circle with different diameters.

[0030] In this embodiment, the cross-sectional view of the container 1 is symmetrically stepped, the top view of the stepped circular container is circles of different sizes, and the bottom surface of the container 1 is a large circle.

[0031] This embodiment ensures the axial symmetry of the container while maintaining the stepped shape of the container. The mold for making the container is simple during the production process, the cost is low, and the container is easy to place.

[0032] This embodiment can be used in multiple environments for testing the density of microalgae, such as inside a large testing instrument, a portable outdoor testing instrument, etc.

[0033] Embodiment 2

[0034] like Figure 1 As shown, a container for detecting microalgae density according to a preferred embodiment of the utility model, the container 1 is a stepped container, and the top view of the container 1 is a circle with different diameters.

[0035] Furthermore, a small opening is opened at the top of the container 1 .

[0036] There is a screw-shaped lid on the small mouth.

[0037] The top of the container 1 is provided with a small opening and a spiral cover, which is convenient for quantitatively placing the microalgae solution into the container 1 and can also keep the microalgae solution isolated from the external environment. The microalgae solution can be placed by dripping with a dropper, or by flowing into a funnel, or by pouring into other containers. The total amount of the microalgae solution is determined according to the needs of the test, and it does not need to be filled up, and the total amount of the microalgae solution does not need to be kept constant each time the microalgae solution is tested.

[0038] Optionally, the container 1 is made of a transparent acrylic plate, glass or quartz stone.

[0039] The material in this embodiment can be made of ordinary acrylic thin plate, which has high light transmittance, is easy to form a film, has low cost, and is convenient to carry and replace.

[0040] Embodiment 3

[0041] The utility model also provides a device for detecting the density of microalgae, including the container 1, power supply 2, luminous light source 3, photoelectric detector 4 and signal processor 7 based on the above, the container 1 is located between the luminous light source 3 and the photoelectric detector 4, the luminous light source 3 is opposite to the receiving surface of the photoelectric detector 4, the power supply 2 is electrically connected with the luminous light source 3 and the photoelectric detector 4, the luminous light source 3 is communicatively connected with the photoelectric detector 4, and the photoelectric detector 4 is communicatively connected with the signal processor 7. After receiving the optical signal, the photoelectric detector 4 transmits the optical signal to the signal processor 7, and then the signal processor 7 calculates and converts the optical signal to the density of microalgae, and finally obtains the data of the density of microalgae.

[0042] In this embodiment, the stepped circular container is used to hold the microalgae solution to be detected and is placed between the light source 3 and the photodetector 4. The power supply 2 is used to provide power to the light source 3 and the photodetector 4. The light source 3 is used to emit light for detection, the photodetector 4 is used to receive and detect the light signal emitted by the light source 3, and the signal processor 7 is used to calculate the density of the microalgae solution through the received light signal.

[0043] The stepped circular container is placed between the light source 3 and the photodetector 4, wherein the receiving surfaces of the light source 3 and the photodetector 4 need to be aligned to ensure that light is emitted from the light source 3, passes through the container 1 and then reaches the receiving surface of the photodetector 4.

[0044] The utility model has expandability, and the stepped circular container can be a single container, and can also be designed as a part connected to a microalgae cultivation device, and is used for long-term monitoring of microalgae density.

[0045] Embodiment 4

[0046] The utility model also provides a device for detecting the density of microalgae, including the above-mentioned container 1, power supply 2, light source 3, photodetector 4 and signal processor 7, the container 1 is located between the light source 3 and the photodetector 4, the light source 3 is opposite to the receiving surface of the photodetector 4, the power supply 2 is electrically connected to the light source 3 and the photodetector 4, the light source 3 is communicatively connected to the photodetector 4, and the photodetector 4 is communicatively connected to the signal processor 7.

[0047] This embodiment further includes a container support 6, which cooperates with the surface of the container 1 to maintain the balance of the container 1 during the test process at different angles. The container support 6 can be used to maintain the vertical incidence of the test light during the placement of the container 1.

[0048] This embodiment further includes a lifting mechanism 5, which is used to adjust the height of the container 1. During the placement of the container 1, the lifting mechanism 5 can be used to adjust the test height and the incident position of the incident light.

[0049] The lifting mechanism 5 is used to adjust the height position of the stepped circular container and the container support 6 .

[0050] The use of this embodiment is flexible and changeable, and light can be incident on different surfaces of the stepped circular container: on the one hand, light can be incident from the top of the stepped circular container, and the light source 3-photodetector 4 combination only needs to be aligned with the planes of different steps to achieve proportional adjustment of the distance the light passes through the microalgae solution; on the one hand, light can be incident from the side of the stepped circular container, and the light source 3-photodetector 4 combination only needs to be aligned with the side of one of the steps on the side to achieve proportional adjustment of the distance the light passes through the microalgae solution; on the other hand, light can be incident from the bottom of the stepped circular container, and the light source 3 needs to be aligned with the bottom surface of the stepped circular container to ensure that the corresponding photodetector 4 is located at the top of the stepped circular container, and the connecting line of the light source 3-photodetector 4 combination vertically passes through the plane of one of the steps of the stepped circular container. In the process of moving the light source 3-photodetector 4 combination, the distance the light passes through the microalgae solution can be adjusted proportionally.

[0051] The light source 3 may be a laser, a light emitting diode (LED), a micro-LED or a mini-LED. Preferably, the light source 3 may be a laser to obtain a thinner light beam for the microalgae density test of a small-scale stepped circular container. If the light source uses a diffuse light source such as LED, micro-LED or mini-LED, a collimator may be added before the test to obtain a parallel light beam.

[0052] The photodetector 4 may be a PIN diode or an APD diode, etc. Preferably, an APD may be used as the photodetector to obtain a better amplification effect and a faster response test result.

[0053] Optical signals generally refer to the detection of light intensity, that is, by judging the change of light intensity, the change trend of microalgae concentration can be obtained. New methods can also be designed to use other parameters of optical signals for detection, such as frequency detection, phase detection, etc.

[0054] The power source 2 can be a constant current source or a constant voltage source, which is selected according to the requirements of the light source and the photoelectric detector. The signal processor 7 can be a mobile terminal such as a mobile phone, a tablet, or a computer.

[0055] like Figure 2 , Figure 3 , Figure 4 As shown, the use of this embodiment is flexible and changeable, and light can be incident on different surfaces of the stepped circular container: during the test, the light source 3 can be aimed at the top of the stepped circular container, the side of the stepped circular container, or the bottom of the stepped circular container. When light is incident from the top of the stepped circular container, it is only necessary to align the light source 3-photodetector 4 combination with the planes of different steps to achieve proportional adjustment of the distance the light passes through the microalgae solution. When light is incident from the side of the stepped circular container, it is only necessary to align the light source 3-photodetector 4 combination with the side of one of the steps on the side to achieve proportional adjustment of the distance the light passes through the microalgae solution. During this process, the stepped circular container needs to be fixed with a clamp to keep the position unchanged. When light is incident from the bottom of the stepped circular container, it is only necessary to align the light source 3 with the bottom surface of the stepped circular container, ensure that the corresponding photodetector 4 is located at the top of the stepped circular container, and the connecting line of the light source-photodetector combination vertically passes through the plane of a step of the stepped circular container. In the process of moving the light source 3-photodetector 4 combination, the proportional adjustment of the distance that the light passes through the microalgae solution can be achieved.

[0056] The test object of the utility model is extensible and can be used for density detection of different microalgae solutions, and can also be used for density detection of other suspended biological solvents and small particle suspensions.

[0057] The total amount of microalgae solution required for the test of the utility model does not need to be fixed or measured. The amount of microalgae solution can be determined according to the test needs. The total amount of microalgae solution measured each time does not need to be unified. The utility model solves the problem that the density of microalgae cannot be detected by traditional microalgae density detection methods and devices when the concentration of microalgae is too high or too low. Through the design of the stepped circular container, a shorter light transmission distance can be used for testing when the concentration of the microalgae sample is too high, and a longer light transmission distance can be used for testing when the concentration of the microalgae sample is too low, thereby ensuring the availability of the test results.

[0058] In summary, the embodiment of the utility model provides a container and device for detecting the density of microalgae, which uses a stepped circular container, which, on the one hand, ensures that the incident light can be collimated under certain conditions, meeting the reliability requirement; on the other hand, it ensures that the distance that the incident light passes through the microalgae solution can be adjusted proportionally, meeting the adjustability requirement, and can realize the analysis and calculation of the density test results of the microalgae solution under different optical path conditions during the test process, obtain the error analysis results, and can also optimize and change the test conditions on this basis.

[0059] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and substitutions can be made without departing from the technical principle of the present invention. These improvements and substitutions should also be regarded as the protection scope of the present invention.

Claims

1. A container for detecting microalgae density, characterized in that: The container (1) is a stepped container, and the top view of the container (1) is a circle with different diameters.

2. A container for detecting microalgae density according to claim 1, characterized in that: The top of the container (1) is provided with a small opening.

3. A container for detecting microalgae density according to claim 2, characterized in that: The small opening is provided with a spiral cover.

4. A container for detecting microalgae density according to claim 3, characterized in that: The container (1) is made of a transparent acrylic plate, glass or quartz stone.

5. A device for detecting microalgae density, characterized in that: The invention comprises a container (1) according to claim 1, a power source (2), a luminous light source (3), a photodetector (4) and a signal processor (7), wherein the container (1) is located between the luminous light source (3) and the photodetector (4), the receiving surfaces of the luminous light source (3) and the photodetector (4) are opposite, the power source (2) is electrically connected to the luminous light source (3) and the photodetector (4), the luminous light source (3) is communicatively connected to the photodetector (4), and the photodetector (4) is communicatively connected to the signal processor (7).

6. A device for detecting microalgae density according to claim 5, characterized in that: It also comprises a container support (6), wherein the container support (6) cooperates with the surface of the container (1) and is used to maintain the balance of the container (1) during the testing process at different angles.

7. The device for detecting microalgae density according to claim 5, characterized in that: It also comprises a lifting mechanism (5), wherein the lifting mechanism (5) is used to adjust the height position of the container (1).

8. The device for detecting microalgae density according to claim 5, characterized in that: The luminous light source (3) is a laser, a light emitting diode (LED), a micro-LED or a mini-LED.

9. The device for detecting microalgae density according to claim 5, characterized in that: The photodetector (4) is a PIN diode or an APD diode.

10. The device for detecting microalgae density according to claim 5, characterized in that: The power supply (2) device is a constant current source or a constant voltage source, and the signal processor (7) is a mobile terminal.

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