Ultramicro detection plate

Abstract

The utility model relates to the technical field of biological detection, in particular to an ultramicro detection plate which comprises a lower fixing support, an upper fixing support is hinged to the lower fixing support, quartz slide fixing windows are arranged on the lower fixing support and the upper fixing support respectively, a first quartz slide is arranged on the lower quartz slide fixing window, and a second quartz slide is arranged on the lower quartz slide fixing window. A porous cavity is formed in the first quartz slide. Through scientific design and reasonable layout, samples are concentrated on one quartz glass plate, and the distance between the upper quartz glass plate and the lower quartz glass plate is controlled, so that absorption light and fluorescence detection of nucleic acid and protein can be quickly carried out in a high-flux and ultramicro manner, and the problem that the detection limit of a traditional cuvette method is too narrow is solved; meanwhile, the limitation that a traditional ultramicro detection plate in the market can only detect 16, 24 and 48 samples at a time is avoided, one-step whole-plate transfer detection of the PCR plate is achieved, and the limitation of a traditional fixing process is broken through.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to an ultra-micro detection plate. Background Art

[0002] The ultra-trace nucleic acid protein quantitative detection plate is mainly used for rapid quantification of ultra-trace nucleic acid protein samples. It can detect 16 or 48 trace samples with a volume of 2μL at a time without dilution, and ensures excellent accuracy and precision.

[0003] However, the ultra-micro nucleic acid and protein quantitative detection plates currently on the market have the problem of too narrow detection range of the colorimetric cup method when used. At the same time, traditional ultra-micro detection plates on the market can only fix a single colorimetric cup, which has certain limitations in use. Utility Model Content

[0004] The purpose of the utility model is to provide an ultra-micro detection plate to solve the problems raised in the above background technology.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] An ultra-micro detection plate, comprising: a lower fixed bracket, an upper fixed bracket is hinged on the lower fixed bracket, the lower fixed bracket and the upper fixed bracket are both provided with quartz glass slide fixing windows, a first quartz glass slide is provided on the lower quartz glass slide fixing window, a porous chamber is provided on the first quartz glass slide, a second quartz glass slide is provided on the upper quartz glass slide fixing window, a sealing mechanism is provided on the second quartz glass slide, a magnet block 1 is embedded on the left side of the top of the lower fixed bracket, a magnet block 2 is embedded on the left side of the bottom of the upper fixed bracket, and two optical path adjustment top strips are provided on the lower fixed bracket, and are respectively located on the front and rear sides of the magnet block 1;

[0007] The sealing mechanism comprises: a sealing tube, the sealing tube is fixedly connected to the bottom of the second quartz glass slide, and the bottom end of the sealing tube is plugged into the top of the first quartz glass slide. The porous chamber is 8 rows and 12 columns of small holes arranged at equal intervals. Through scientific design and reasonable layout, 96 samples are concentrated on a quartz glass plate, and the distance between the upper and lower quartz glass slides is controlled, so that the absorption light and fluorescence detection of nucleic acids and proteins can be carried out quickly, with high throughput and in ultra-trace quantities, avoiding the problem of too narrow detection limit of the traditional colorimetric cup method, and also avoiding the limitation that the traditional ultra-trace detection plate on the market can only detect 16, 24, and 48 samples at a time, realizing the one-step whole-plate transfer detection of the PCR plate, breaking through the limitation of the traditional fixing process, adjusting the optical path distance between the upper and lower quartz glass slides by the optical path adjustment top strip, which can further expand the detection limit range and broaden the application field, and can keep the overall gap between the upper and lower plates uniform and uniform by magnet adsorption closure, and can seal the surrounding area of ​​the porous chamber by the provided sealing tube, thereby preventing the detection sample from overflowing, which brings convenience to the detection work.

[0008] Preferably, an annular slot is provided on the top of the first quartz glass slide and is located outside the porous chamber, and the bottom end of the sealing tube is inserted into the slot, so that the sealing of the sealing tube is facilitated by the provided slot.

[0009] Preferably, the lower fixing bracket is provided with a first fixing mechanism, the first fixing mechanism comprises: a slide groove, a fixing block, and a screw rod, the slide groove is opened on the front inner wall of the lower quartz glass slide fixing window, the fixing block is slidably connected in the slide groove, and the rear of the fixing block is clamped with the first quartz glass slide, the screw rod is threadedly connected to the front side of the lower fixing bracket, and the rear end of the screw rod passes through the lower fixing bracket and is rotatably connected to the front side of the fixing block, and the fixing block is driven to slide along the inner wall of the slide groove by rotating the screw rod, so that the first quartz glass slide is disengaged from the clamping groove, thereby releasing the fixation of the first quartz glass slide, facilitating the installation and disassembly of the first quartz glass slide, and facilitating the replacement or cleaning and maintenance of the first quartz glass slide.

[0010] Preferably, a card slot is provided on the back side of the fixing block, and the first quartz glass slide is snapped into the card slot, so that the first quartz glass slide can be fixed by the provided card slot.

[0011] Preferably, a second fixing mechanism is provided on the upper fixing bracket, and the structure of the second fixing mechanism is completely the same as that of the first fixing mechanism. The second fixing mechanism is provided to facilitate replacement or cleaning and maintenance of the second quartz glass slide.

[0012] Preferably, the first magnet block and the second magnet block attract each other with opposite polarities.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] 1. Through scientific design and reasonable layout, 96 samples are concentrated on a quartz glass plate, and the distance between the upper and lower quartz glass slides is controlled, so that the absorption light and fluorescence detection of nucleic acids and proteins can be carried out quickly, with high throughput and ultra-trace, avoiding the problem of too narrow detection limit of the traditional colorimetric cup method, and also avoiding the limitation that the traditional ultra-trace detection plate on the market can only detect 16, 24, and 48 samples at a time, and realizing the one-step transfer detection of the PCR plate, breaking through the limitations of the traditional fixation process. The optical path distance between the upper and lower quartz glass slides can be adjusted by adjusting the optical path adjustment top bar, which can further expand the detection limit range and broaden the application field. The overall gap between the upper and lower plates can be kept uniform by magnetic adsorption closure, and the surrounding area of ​​the porous chamber can be sealed by the set sealing tube, thereby preventing the overflow of the detection sample, which brings convenience to the detection work;

[0015] 2. The screw is rotated to drive the fixing block to slide along the inner wall of the slide groove, so that the first quartz glass slide is disengaged from the slot, thereby releasing the fixation of the first quartz glass slide, facilitating the installation and disassembly of the first quartz glass slide, and facilitating the replacement or cleaning and maintenance of the first quartz glass slide. The second fixing mechanism is provided to facilitate the replacement or cleaning and maintenance of the second quartz glass slide. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0017] Figure 2 It is a top view of the lower fixing bracket in the utility model;

[0018] Figure 3 This is a bottom view of the upper fixing bracket in the utility model;

[0019] Figure 4 It is a left side cross-sectional view of the utility model.

[0020] In the figure: 1. lower fixing bracket; 2. upper fixing bracket; 3. quartz glass slide fixing window; 4. first quartz glass slide; 5. porous chamber; 6. second quartz glass slide; 7. sealing mechanism; 71. sealing tube; 72. slot; 8. first fixing mechanism; 81. slide groove; 82. fixing block; 83. screw; 84. slot; 9. magnet block one; 10. magnet block two; 11. optical path adjustment top strip; 12. second fixing mechanism. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] like Figure 1-4 As shown, an ultra-micro detection plate comprises: a lower fixed bracket 1, an upper fixed bracket 2 is hinged on the lower fixed bracket 1, a quartz glass fixing window 3 is arranged on the lower fixed bracket 1 and the upper fixed bracket 2, a first quartz glass slide 4 is arranged on the lower quartz glass fixing window 3, a porous chamber 5 is arranged on the first quartz glass slide 4, a second quartz glass slide 6 is arranged on the upper quartz glass fixing window 3, a sealing mechanism 7 is arranged on the second quartz glass slide 6, and a magnet block is embedded in the left side of the top of the lower fixed bracket 1. 9, a magnet block 10 is embedded in the left side of the bottom of the upper fixed bracket 2, and two optical path adjustment top strips 11 are arranged on the lower fixed bracket 1, and are respectively located on the front and rear sides of the magnet block 9; the sealing mechanism 7 includes: a sealing tube 71, the sealing tube 71 is fixedly connected to the bottom of the second quartz glass slide 6, and the bottom end of the sealing tube 71 is plugged into the top of the first quartz glass slide 4, the top of the first quartz glass slide 4 is provided with an annular slot 72, and is located outside the porous chamber 5, and the bottom end of the sealing tube 71 is plugged into the slot 72.

[0023] Specifically, the porous chamber 5 is composed of 8 rows and 12 columns of small holes arranged at equal intervals. Through scientific design and reasonable layout, 96 samples are concentrated on a quartz glass plate, and the distance between the upper and lower quartz glass slides is controlled, so that the absorption light and fluorescence detection of nucleic acids and proteins can be carried out quickly, with high throughput and in ultra-trace quantities, avoiding the problem of too narrow detection limit of the traditional colorimetric cup method, and also avoiding the limitation that the traditional ultra-trace detection plate on the market can only detect 16, 24, and 48 samples at a time, realizing the one-step transfer detection of the PCR plate, breaking through the limitation of the traditional fixing process, and adjusting the optical path distance between the upper and lower quartz glass slides by adjusting the optical path adjustment top strip 11, which can further expand the detection limit range and broaden the application field, the magnet block 1 9 and the magnet block 2 10 are attracted to each other by opposite poles, and the overall gap between the upper and lower plates can be kept uniform by magnet adsorption closure, and the sealing of the sealing tube 71 is facilitated by the provided slot 72, and the surrounding area of ​​the porous chamber 5 can be sealed by the provided sealing tube 71, thereby preventing the detection sample from overflowing, which brings convenience to the detection work.

[0024] like Figure 1-4As shown, the lower fixed bracket 1 is provided with a first fixing mechanism 8, and the first fixing mechanism 8 includes: a slide groove 81, a fixing block 82, and a screw 83. The slide groove 81 is opened on the front of the inner wall of the quartz glass slide fixing window 3 below, the fixing block 82 is slidably connected in the slide groove 81, and the rear of the fixing block 82 is clamped with the first quartz glass slide 4, the screw 83 is threadedly connected to the front of the lower fixed bracket 1, and the rear end of the screw 83 passes through the lower fixed bracket 1 and is rotatably connected with the front of the fixing block 82. The upper fixed bracket 2 is provided with a second fixing mechanism 12, and the structure of the second fixing mechanism 12 is exactly the same as that of the first fixing mechanism 8.

[0025] Specifically, a card slot 84 is provided on the back of the fixing block 82, and the first quartz glass slide 4 is carded in the card slot 84. The first quartz glass slide 4 can be fixed by the card slot 84. The fixing block 82 is driven to slide along the inner wall of the slide groove 81 by rotating the screw 83, so that the first quartz glass slide 4 is disengaged from the card slot 84, thereby releasing the fixation of the first quartz glass slide 4, facilitating the installation and disassembly of the first quartz glass slide 4, and facilitating the replacement or cleaning and maintenance of the first quartz glass slide 4; the second fixing mechanism 12 is provided to facilitate the replacement or cleaning and maintenance of the second quartz glass slide 6.

[0026] The working principle of the utility model is as follows: through scientific design and reasonable layout, 96 samples are concentrated on a quartz glass plate, and the distance between the upper and lower quartz glass slides is controlled, so that the absorption light and fluorescence detection of nucleic acids and proteins can be carried out quickly, with high throughput and in ultra-trace quantities, avoiding the problem of too narrow detection limit of the traditional colorimetric cup method, and also avoiding the limitation that the traditional ultra-trace detection plate on the market can only detect 16, 24, and 48 samples at a time, realizing the one-step transfer detection of the PCR plate, breaking through the limitation of the traditional fixing process, and adjusting the optical path distance between the upper and lower quartz glass slides by the optical path adjustment top strip 11, which can further expand the detection limit range. The invention can broaden the application field, and the overall gap between the upper and lower plates can be kept uniform by magnet adsorption closure. The surrounding area of ​​the porous chamber 5 can be sealed by the provided sealing tube 71, so as to prevent the detection sample from overflowing, which brings convenience to the detection work; the fixing block 82 is driven to slide along the inner wall of the slide groove 81 by rotating the screw 83, so that the first quartz glass slide 4 is separated from the card groove 84, thereby releasing the fixation of the first quartz glass slide 4, facilitating the installation and disassembly of the first quartz glass slide 4, and facilitating the replacement or cleaning and maintenance of the first quartz glass slide 4. Similarly, the provided second fixing mechanism 12 facilitates the replacement or cleaning and maintenance of the second quartz glass slide 6.

[0027] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the utility model and are not used to limit the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection of the utility model is defined by the attached claims and their equivalents.

Claims

1. An ultra-micro detection plate, characterized in that: include: A lower fixed support (1), the lower fixed support (1) is hingedly connected to an upper fixed support (2), the lower fixed support (1) and the upper fixed support (2) are both provided with quartz glass slide fixing windows (3), the lower quartz glass slide fixing window (3) is provided with a first quartz glass slide (4), the first quartz glass slide (4) is provided with a porous chamber (5), the upper quartz glass slide fixing window (3) is provided with a second quartz glass slide (6), the second quartz glass slide (6) is provided with a sealing mechanism (7), a magnet block 1 (9) is embedded on the left side of the top of the lower fixed support (1), a magnet block 2 (10) is embedded on the left side of the bottom of the upper fixed support (2), and two optical path adjustment top strips (11) are provided on the lower fixed support (1), and are respectively located on the front and rear sides of the magnet block 1 (9); The sealing mechanism (7) comprises: a sealing tube (71), wherein the sealing tube (71) is fixedly connected to the bottom of the second quartz glass slide (6), and the bottom end of the sealing tube (71) is plugged into the top of the first quartz glass slide (4).

2. The ultra-micro detection plate according to claim 1, characterized in that: The top of the first quartz glass slide (4) is provided with an annular slot (72) which is located outside the porous chamber (5), and the bottom end of the sealing tube (71) is inserted into the slot (72).

3. The ultra-micro detection plate according to claim 1, characterized in that: The lower fixing bracket (1) is provided with a first fixing mechanism (8), the first fixing mechanism (8) comprising: a slide groove (81), a fixing block (82), and a screw rod (83); the slide groove (81) is opened on the front side of the inner wall of the lower quartz glass slide fixing window (3); the fixing block (82) is slidably connected in the slide groove (81), and the rear of the fixing block (82) is clamped with the first quartz glass slide (4); the screw rod (83) is threadedly connected to the front side of the lower fixing bracket (1), and the rear end of the screw rod (83) passes through the lower fixing bracket (1) and is rotatably connected to the front side of the fixing block (82).

4. The ultra-micro detection plate according to claim 3, characterized in that: The back of the fixing block (82) is provided with a slot (84), and the first quartz glass slide (4) is snapped into the slot (84).

5. The ultra-micro detection plate according to claim 1, characterized in that: The upper fixing bracket (2) is provided with a second fixing mechanism (12), and the structure of the second fixing mechanism (12) is completely the same as that of the first fixing mechanism (8).

6. The ultra-micro detection plate according to claim 1, characterized in that: The magnet block 1 (9) and the magnet block 2 (10) attract each other with opposite polarities.

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