Nucleic acid sequencing system

Abstract

The utility model provides a nucleic acid sequencing system. The nucleic acid sequencing system comprises a chip, a reagent storage container, a waste liquid storage container, a diversion system, a detection system, a computer system and a monitoring system, one or more sequencing objects are attached to the chip; the diversion system is configured to controllably convey one or more reagents from the reagent storage container into the chip through the first liquid channel so as to be in contact with a sequencing object and generate a chemical reaction, and controllably convey waste liquid generated after the chemical reaction from the chip into the waste liquid storage container through the second liquid channel; the computer system is configured to obtain the nucleic acid feature information from the detection system and identify a nucleic acid sequence of a sequencing object according to the nucleic acid feature information; the monitoring system is configured to detect liquid pressure when the reagent flows through the first liquid channel and / or the waste liquid flows through the second liquid channel. According to the utility model, the blocking degree of the liquid channel can be effectively detected and checked when the sequencing system is installed or operated.

Description

Technical Field

[0001] The utility model relates to the technical field of gene sequencing, and in particular to a nucleic acid sequencing system. Background Art

[0002] Nucleic acid sequencing systems are widely used in genomics, genetics, disease research and other scenarios. They help to gain a deeper understanding of the genetic characteristics and evolutionary history of organisms, help to discover disease-related genetic variation, and assist in disease detection and treatment.

[0003] In the 1970s, the first generation of sequencing technology was introduced. As people's demand for low-cost and high-throughput sequencing increased day by day, high-throughput sequencing was introduced. High-throughput sequencing (HTS) technology is also called the second generation sequencing (NGS) technology, represented by polymerase sequencing Solexa, pyrophosphate sequencing MostafaRonaghi and ligase sequencing Drmanac R-Sparks AB. Although the third generation sequencing technology has been introduced in the past two years, the third generation sequencing technology is not mature enough, resulting in its sequencing accuracy not as good as the second generation sequencing technology. Therefore, the second generation sequencing technology is still the most widely used.

[0004] In a nucleic acid sequencing system using the second generation sequencing technology, various bases in the nucleic acid are identified by chemically reacting one or more reagents with the sequencing object (e.g., nucleic acid fragments) on the chip. A liquid channel is provided in the nucleic acid sequencing system to transport the reagents from the storage container to the chip, and to discharge the waste liquid after the chemical reaction into a waste liquid container (e.g., a waste liquid box) for storage. Liquid (reagents or waste liquid) will remain in the liquid channel after use, and the liquid remaining in the liquid channel may also block the liquid channel due to crystallization. Therefore, it is necessary to detect and troubleshoot the degree of blockage of the liquid channel when the sequencing system is installed or in operation. Therefore, it is urgent to provide relevant solutions. Utility Model Content

[0005] In view of this, an object of the embodiments of the present invention is to provide a nucleic acid sequencing system to at least partially solve the above technical problems.

[0006] The present invention provides a nucleic acid sequencing system, including: a chip, a reagent storage container, a waste liquid storage container, a flow diversion system, a detection system, a computer system and a monitoring system; wherein:

[0007] One or more sequencing objects are attached to the chip;

[0008] The reagent storage container is configured to store one or more reagents that can chemically react with the sequencing object;

[0009] The waste liquid storage container is configured to store waste liquid after chemical reaction with the sequencing object;

[0010] The fluid guiding system is configured to controllably transport the one or more reagents from the reagent storage container to the chip through the first liquid channel so as to contact with the sequencing object and undergo a chemical reaction, and controllably transport waste liquid after the chemical reaction from the chip to the waste liquid storage container through the second liquid channel;

[0011] The detection system is configured to detect nucleic acid characteristics characterized by the sequencing object after chemical reaction with the one or more reagents;

[0012] The computer system is configured to obtain nucleic acid feature information from the detection system, and identify the nucleic acid sequence of the sequencing object according to the nucleic acid feature information;

[0013] The monitoring system is configured to detect the liquid pressure when the reagent flows through the first liquid channel and / or the waste liquid flows through the second liquid channel, and to provide a prompt for the detected liquid pressure.

[0014] In a possible implementation, the fluid guiding system is further configured to controllably transport the one or more reagents from the reagent storage container to the waste liquid storage container through a third liquid channel;

[0015] The monitoring system is also configured to detect the liquid pressure when the reagent flows through the third liquid channel, and to provide a prompt for the detected liquid pressure.

[0016] In a possible implementation, the monitoring system is further configured to detect the gas pressure when gas is introduced into at least one of the first liquid channel, the second liquid channel, and the third liquid channel, and to provide a prompt for the detected gas pressure.

[0017] In a possible implementation, the monitoring system includes a pressure detection device and a control device, wherein the control device is configured to generate corresponding prompt information according to the pressure value detected by the pressure detection device, and can control the operation of the nucleic acid sequencing system based on the detected pressure value, wherein the pressure detection device includes:

[0018] at least one pressure sensor, electrically connected to the processing module, configured to sense the liquid pressure when the reagent flows through the first liquid channel and / or the waste liquid flows through the second liquid channel, and output a first voltage to be measured representing a change in liquid pressure;

[0019] A voltage conversion module, the input end of which is electrically connected to the external power supply, the output end of which is electrically connected to the processing module, and is configured to convert the input voltage provided by the external power supply into a reference voltage and then output it;

[0020] The processing module is configured to determine and output a value of the liquid pressure sensed by the pressure sensor according to the first voltage to be measured and the reference voltage.

[0021] In a possible implementation, the temperature acquisition module is configured to acquire temperature data of the environment in which the pressure detection device is located, and generate a corresponding temperature signal according to the acquired temperature data;

[0022] The processing module is further configured to compensate the value of the liquid pressure according to the temperature signal to obtain a compensated value of the liquid pressure.

[0023] In a possible embodiment, the processing module is also provided with a communication interface, which is configured to send the value of the liquid pressure to the control device; the communication interface includes at least one of an integrated circuit bus interface, a universal asynchronous receiver and transmitter interface, a serial communication interface and a controller area network interface.

[0024] In a possible implementation, the pressure detection device further includes a data storage module, wherein the data storage module is configured to store calibration data for each of the pressure sensors, wherein the calibration data is configured to calibrate the value of the liquid pressure collected by the corresponding pressure sensor.

[0025] In a possible implementation, the processing module further provides an address configuration interface, and the address configuration interface is configured to output an object address of each of the pressure sensors, wherein the object addresses of different sensors are different, so that different sensors can be distinguished by the object addresses.

[0026] In a possible implementation, the pressure detection device further includes a level conversion module; the communication interface includes the integrated circuit bus interface;

[0027] The level conversion module is connected to the processing module via the integrated circuit bus interface, and the level conversion module is configured to increase the value of the liquid pressure output by the processing module via the integrated circuit bus interface to a preset threshold value and then output it to the control device.

[0028] In one possible implementation, the monitoring system is further configured to prompt an abnormal situation when the detected liquid pressure and / or gas pressure is abnormal.

[0029] In a possible implementation, the monitoring system is further configured to collect and control the temperature of at least one of the chip and the reagent.

[0030] In the embodiment of the utility model, since the nucleic acid sequencing system is provided with a monitoring system, and the monitoring system is configured to detect the liquid pressure when the reagent flows through the first liquid channel and / or the waste liquid flows through the second liquid channel, the liquid pressure detected by the monitoring system can reflect the degree of blockage of the liquid channel, thereby achieving the purpose of detecting and troubleshooting the liquid channel. Furthermore, the monitoring system can also provide effective prompts when the liquid pressure is abnormal.

[0031] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, preferred embodiments are given below and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0033] Figure 1 A principle block diagram of a nucleic acid sequencing system provided by some embodiments of the present utility model is shown;

[0034] Figure 2 The principle block diagram of the nucleic acid sequencing system provided by other embodiments of the utility model is shown;

[0035] Figure 3 The principle block diagram of the pressure detection device provided by some embodiments of the utility model is shown;

[0036] Figure 4 The schematic diagram of the pressure detection device provided by other embodiments of the utility model is shown. DETAILED DESCRIPTION

[0037] In order to make the purpose, technical scheme and advantages of the embodiment of the utility model clearer, the technical scheme in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is only a part of the embodiment of the utility model, rather than all the embodiments. The components of the embodiment of the utility model generally described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiment of the utility model provided in the drawings is not intended to limit the scope of the utility model for protection, but merely represents the selected embodiment of the utility model. Based on the embodiment of the utility model, all other embodiments obtained by those skilled in the art without making creative work belong to the scope of protection of the utility model.

[0038] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0039] The term "and / or" herein only describes an association relationship, indicating that three relationships may exist. For example, A and / or B may represent the following three situations: A exists alone, A and B exist at the same time, and B exists alone. In addition, the term "at least one" herein represents any combination of at least two of any one or more of a plurality of. For example, including at least one of A, B, and C may represent including any one or more elements selected from the set consisting of A, B, and C.

[0040] In a nucleic acid sequencing system using the second generation sequencing technology, various bases in the nucleic acid are identified by chemically reacting one or more reagents with the sequencing object (e.g., nucleic acid fragment) on the chip. Specifically, a liquid channel is provided in the nucleic acid sequencing system to transport the reagent from the storage container to the chip, and to discharge the waste liquid after the chemical reaction into a waste liquid container (e.g., a waste liquid box) for storage.

[0041] However, research has found that liquid (reagents or waste liquid) will remain in the liquid channel after use, and the liquid remaining in the liquid channel may also block the liquid channel due to crystallization. Therefore, it is necessary to detect and check the degree of blockage of the liquid channel when the sequencing system is installed or running, so as to avoid affecting the normal sequencing due to blockage of the liquid channel.

[0042] Based on the above research, the utility model provides a nucleic acid sequencing system, which is equipped with a monitoring system, and the monitoring system is configured to detect the liquid pressure when the reagent flows through the first liquid channel and / or the waste liquid flows through the second liquid channel. In this way, the liquid pressure detected by the monitoring system can reflect the degree of blockage of the liquid channel, achieving the purpose of detecting and troubleshooting the liquid channel. Furthermore, the monitoring system can also generate a first prompt message when the liquid pressure is abnormal, so that not only can the user intuitively perceive whether an abnormality occurs in the liquid channel, but also the timeliness of abnormality perception can be improved.

[0043] In addition, the monitoring system can also take corresponding measures (such as clearing the obstruction of the liquid channel) when the liquid channel is abnormally blocked, so as to reduce the occurrence of the system not being able to work normally due to insufficient fluidity of the liquid channel.

[0044] The defects existing in the above solutions are the results obtained by the inventor after practice and careful research. Therefore, the discovery process of the above problems and the solutions proposed by the present invention for the above problems below should be the contributions made by the inventor to the present invention in the process of the present invention.

[0045] The following is a detailed description of the nucleic acid sequencing system provided by the embodiment of the utility model in conjunction with the accompanying drawings. Figure 1 , which is a principle block diagram of a nucleic acid sequencing system 100 provided by some embodiments of the present invention. The nucleic acid sequencing system 100 includes a chip 110, a reagent storage container 120, a flow guiding system 130, a waste liquid storage container 140, a detection system 150, a computer system 160 and a monitoring system 170.

[0046] Specifically, one or more sequencing objects are attached to the chip 110. The sequencing objects are derived from gene sequencing samples. In some possible embodiments, the gene sequencing samples may be, for example, blood, cell tissue, etc. of an organism. Deoxyribonucleotide (DNA) single strand or ribonucleotide (RNA) single strand may be extracted and separated from the gene sequencing sample.

[0047] In some other possible embodiments, the sequencing object may be, for example, a single DNA strand or a single RNA strand cut into multiple fragments, and then chemically modified and joined to the DNA fragments or RNA fragments, that is, the sequencing object may be a gene library obtained through a library preparation process, or the sequencing object may be an entire single DNA strand or an entire single RNA strand, wherein the RNA double strand may be obtained by transcribing the DNA double strand.

[0048] The reagent storage container 120 is configured to store one or more reagents that react chemically with the sequencing object, wherein the reagents at least include sequencing reagents, and in some implementations, the reagents may also include clustering reagents.

[0049] The fluid guiding system 130 is configured to controllably transport the one or more reagents from the reagent storage container 120 to the chip 110 through the first liquid channel so as to contact with the sequencing object and undergo a chemical reaction, and controllably transport the waste liquid after the chemical reaction from the chip 110 to the waste liquid storage container 140 through the second liquid channel. That is, the waste liquid storage container 140 is configured to store the waste liquid.

[0050] Exemplarily, the fluid guiding system 130 may include a liquid channel, a rotary valve, a syringe pump, and the like.

[0051] Specifically, as an implementation, the first liquid channel extends into the reagent storage container 120, and the one or more reagents are drawn from the reagent storage container 120 through a rotary valve, and the one or more drawn reagents are transported to the chip 110 along the first liquid channel. After the reagent chemically reacts with the sequencing object attached to the chip 110, the reagent that has chemically reacted with the sequencing object is transported as waste liquid along the second liquid channel to the waste liquid storage container 140 through a syringe pump.

[0052] The detection system 150 is configured to detect the nucleic acid characteristics characterized by the sequencing object after chemical reaction with the one or more reagents. The computer system 160 is configured to obtain nucleic acid characteristic information from the detection system 150 and identify the nucleic acid sequence of the sequencing object according to the nucleic acid characteristic information.

[0053] Exemplarily, when the sequencing object is at least one of a DNA single strand, a DNA fragment, and a DNA double strand, the sequencing object consists of four bases: adenine A (ADENINE, A), thymine (THYMINE, T), guanine (GUANINE, G), and cytosine (CYTOSINE, C). When the sequencing object is at least one of an RNA single strand, an RNA fragment, and an RNA double strand, the sequencing object consists of four bases: adenine A (ADENINE, A), uracil (Uracil, U), guanine (GUANINE, G), and cytosine (CYTOSINE, C).

[0054] Different bases have different base characteristics after chemically reacting with the reagent. Exemplarily, different bases emit different fluorescent signals after chemically reacting with the reagent. Therefore, the sequencing object composed of different bases has different nucleic acid characteristics after chemically reacting with the reagent. In this way, the computer system 160 can identify the nucleic acid sequence of the sequencing object based on the nucleic acid characteristic information obtained from the detection system 150.

[0055] The monitoring system 170 is configured to detect the liquid pressure when the reagent flows through the first liquid channel and / or the waste liquid flows through the second liquid channel, and to provide a prompt for the detected liquid pressure.

[0056] For example, the monitoring system 170 may include a display module, through which the detected liquid pressure value is prompted. In another possible implementation, the nucleic acid sequencing system also includes a display system ( Figure 1 (not shown), the monitoring system 170 sends the detected liquid pressure value to the display system, and the display system prompts the liquid pressure value.

[0057] In other possible implementations, the monitoring system 170 may also generate first prompt information when the liquid pressure is abnormal, where the first prompt information is used to prompt obstructive abnormalities of the first liquid channel and / or the second liquid channel.

[0058] It can be understood that the first prompt information can be displayed by a display module in the monitoring system 170 or a display system in the nucleic acid sequencing system.

[0059] Optionally, the nucleic acid sequencing system 100 further includes a chip platform (not shown), which is configured to fix and support the chip 110. Exemplarily, the chip platform can be specifically an XY motion platform that can move in the horizontal and vertical directions, respectively, so as to assist the reagent to fully contact the sequencing object.

[0060] The nucleic acid sequencing system 100 provided in the embodiment of the utility model is provided with a monitoring system 170 and the monitoring system 170 is configured to detect the liquid pressure when the reagent flows through the first liquid channel and / or the waste liquid flows through the second liquid channel. In this way, the liquid pressure detected by the monitoring system 170 can reflect the degree of blockage of the liquid channel, thereby achieving the purpose of detecting and troubleshooting the liquid channel. Furthermore, the monitoring system 170 can also generate a first prompt message when the liquid pressure is abnormal, so that not only can the user intuitively perceive whether an abnormality occurs in the liquid channel, but also the timeliness of abnormality perception can be improved.

[0061] See also Figure 2FIG. 1 is a schematic diagram of another nucleic acid sequencing system provided by an embodiment of the present invention. Figure 1 The difference from the embodiment is that the flow guiding system 130 is further configured to controllably transport the one or more reagents from the reagent storage container 120 to the waste liquid storage container 140 through the third liquid channel. The monitoring system 170 is further configured to detect the liquid pressure when the reagent flows through the third liquid channel, and to prompt the detected liquid pressure.

[0062] In other possible implementations, the monitoring system 170 may also generate a second prompt message when the liquid pressure of the third liquid channel is abnormal, and the second prompt message is used to indicate that the obstruction of the third liquid channel is abnormal. In this way, the monitoring system 170 may also detect the obstruction of the third liquid channel, thereby helping to improve the applicability of the nucleic acid sequencing system 100.

[0063] In addition, the monitoring system 170 is further configured to detect the gas pressure when gas is introduced into at least one of the first liquid channel, the second liquid channel and the third liquid channel, and to provide a prompt for the detected gas pressure.

[0064] In other possible implementations, the monitoring system 170 may also generate a third prompt message when the gas pressure is abnormal, and the third prompt message is used to prompt that the air tightness of the liquid channel being tested is abnormal. That is, before the nucleic acid sequencing system 100 is used or after being used for a period of time, gas may be introduced into the first liquid channel, the second liquid channel, and the third liquid channel, and then the air tightness of the liquid channel may be tested and checked by the detected gas pressure, and a prompt message may be issued to remind the user when the air tightness of the liquid channel is abnormal.

[0065] Specifically, the monitoring system 170 includes a pressure detection device 171 and a control device 172, wherein the control device 172 is configured to generate corresponding prompt information according to the pressure value detected by the pressure detection device 171, and can control the operation of the nucleic acid sequencing system 100 based on the detected pressure value. The pressure value may be a gas pressure value or a liquid pressure value.

[0066] Optionally, the monitoring system 170 is further configured to collect and control the temperature of at least one of the chip 110 and the reagent. In this embodiment, the control device 172 can be used to collect and control the temperature of at least one of the chip 110 and the reagent.

[0067] Exemplarily, the pressure detection device 171 includes at least one pressure sensor 10, a processing module 20, and a voltage conversion module 30. The at least one pressure sensor 10 is electrically connected to the processing module 20, and is configured to detect the liquid pressure when the reagent flows through the first liquid channel and / or the waste liquid flows through the second liquid channel, and output a first voltage to be measured that represents the change in liquid pressure. The first voltage to be measured is an analog voltage.

[0068] It can be understood that in other embodiments, the at least one pressure sensor 10 can also be configured to detect the liquid pressure of the third liquid channel, or detect the gas pressure when gas is passed into at least one of the first liquid channel, the second liquid channel and the third liquid channel, and output a corresponding voltage to be measured. For example, the number of the pressure sensors can be three, and corresponding pressure sensors can be respectively provided for the first liquid channel, the second liquid channel, and the third liquid channel.

[0069] The input end of the voltage conversion module 30 is electrically connected to an external power supply (not shown), and the output end is electrically connected to the processing module 20. The voltage conversion module 30 is configured to convert the input voltage provided by the external power supply into a reference voltage and then output it. The processing module 20 is configured to determine and output the value of the liquid pressure sensed by the pressure sensor according to the first voltage to be measured and the reference voltage. That is, the voltage provided by the voltage conversion module 30 is not used to power the processing module 20, but is used to provide a reference voltage for reading data from the pressure sensor 10. Among them, the value of the liquid pressure is a digital signal.

[0070] In the embodiment of the utility model, since the pressure detection device 171 is provided with a processing module 20, the analog signal output by the analog pressure sensor 10 can be converted into a digital signal to obtain a corresponding digital signal. Since the digital signal has a strong stability during the transmission process, it can not only reduce the use cost but also improve the stability of subsequent signal transmission.

[0071] See also Figure 3 As shown, Figure 3 The principle block diagram of another pressure detection device provided by the embodiment of the utility model. The pressure detection device 171 also includes a power supply module 40, the input end of the power supply module 40 is connected to the external power supply, the output end of the power supply module 40 is connected to the processing module 20, and the power supply module 40 is used to convert the input voltage provided by the external power supply into the working voltage required by the processing module 20 and then supply power to the processing module 20.

[0072] Exemplarily, the operating voltage required by the processing module 20 may be 3.3V or 5V, which may be determined according to actual needs. Accordingly, the power supply module 40 may include a DC-DC converter, for example, the power supply module 40 may include a boost circuit, a buck circuit, and a buck-boost circuit, and thus may implement corresponding voltage conversion according to the size of the input voltage.

[0073] In the embodiment of the utility model, since the voltage conversion module 30 used for pressure determination is independently arranged from the power supply module 40, the accuracy of pressure determination is not affected by the accuracy of the power supply module 40, and can be determined according to actual needs, thereby improving the applicability of the pressure detection device 171.

[0074] In some possible implementations, in order to further improve the accuracy of signal conversion, the voltage conversion accuracy of the voltage conversion module 30 is greater than the voltage conversion accuracy of the power supply module 40. For example, the voltage conversion module 30 can use a high-precision voltage-dividing resistor to collect the power supply voltage (the input voltage provided by the external power supply), thereby providing a reference voltage for the data reading of the pressure sensor 10. Of course, in other embodiments, the voltage conversion accuracy of the voltage conversion module 30 can be set according to actual needs, and is not specifically limited here.

[0075] Optionally, the processing module 20 may be a processor, that is, an integrated circuit chip with signal processing capabilities. The processor may be a general-purpose processor, including a central processing unit (CPU), a network processor (NP), etc.; it may also be a digital signal processor (DSP), an application specific integrated circuit (ASIC), a field programmable gate array (FPGA) or other programmable logic devices, discrete gates or transistor logic devices, discrete hardware components. The general-purpose processor may be a microprocessor or the processor may also be any conventional processor, etc.

[0076] In the embodiment of the utility model, the processing module 20 may be a microcontroller unit (MCU). The microcontroller unit may include a plurality of signal acquisition ports, a communication port, a plurality of control ports, and the like.

[0077] In a possible embodiment, the pressure detection device 171 further includes a temperature acquisition module 50. The temperature acquisition module 50 is connected to the processing module 20, and is configured to acquire temperature data of the environment in which the pressure detection device 171 is located, and to generate a corresponding temperature signal according to the acquired temperature data. The processing module 20 is also configured to receive the temperature signal output by the temperature acquisition module 50, and to compensate the value of the liquid pressure according to the temperature signal to obtain the value of the compensated liquid pressure.

[0078] In the embodiment of the utility model, since the pressure detection device 171 also includes a temperature acquisition module 50, the processing module 20 can compensate the data collected by the pressure sensor 10 according to the collected temperature signal, thereby further improving the collection accuracy.

[0079] In some possible embodiments, the pressure detection device 171 further includes a data storage module 60, and the data storage module 60 is configured to store calibration data for each of the pressure sensors 10, and the calibration data is configured to calibrate the value of the liquid pressure collected by the corresponding pressure sensor 10. In this way, the sensing accuracy can be improved by using the storage module 60 to store the calibration data of the pressure sensor 10.

[0080] Among them, the data storage module 60 can be, but is not limited to, random access memory (Random Access Memory, RAM), read only memory (Read Only Memory, ROM), programmable read-only memory (Programmable Read-Only Memory, PROM), erasable programmable read-only memory (Erasable Programmable Read-Only Memory, EPROM), electrically erasable read-only memory (Electric Erasable Programmable Read-Only Memory, EEPROM), etc.

[0081] It can be understood that, in some optional embodiments, each pressure sensor 10 is also provided with a corresponding signal amplification module (not shown in the figure), which can be connected between the corresponding pressure sensor 10 and the processing module 20, and is used to amplify the measured voltage output by the corresponding pressure sensor 10 and output it to the processing module 20.

[0082] Exemplarily, the signal amplification module may include an operational amplifier. Using the operational amplifier to process the analog signal (voltage to be measured) output by the pressure sensor 10 can improve data stability and data driving capability, while taking into account signal isolation to prevent external interference signals from affecting the operation of the processing module 20.

[0083] See also Figure 4 As shown, Figure 4 A principle block diagram of another pressure detection device provided for an embodiment of the utility model. Optionally, the processing module 20 is also provided with a communication interface, and the communication interface is configured to send the value of the liquid pressure to the control device 172. The communication interface includes at least one of an integrated circuit bus (IIC) interface, a universal asynchronous receiver / transmitter (UART) interface, a serial communication interface (such as RS232), and a controller area network (CAN) interface. In this way, data interaction with the control end target device can be performed through the communication interface, thereby improving the compatibility of the pressure detection device 171.

[0084] Among them, UART is a universal serial data bus for asynchronous communication. The bus communicates bidirectionally and can achieve full-duplex transmission and reception. CAN is a serial communication protocol bus for real-time applications. It can use twisted pair to transmit signals. It is one of the field buses and a serial communication network that effectively supports distributed control systems.

[0085] Optionally, the processing module 20 is further provided with an address configuration interface 70, and the address configuration interface 70 is configured to output an object address of each of the pressure sensors, wherein the object addresses of different sensors are different, so that different sensors can be distinguished by the object addresses. Specifically, the object addresses can be configured using multiple resistors, for example, a first address can be configured for the first liquid channel, a second address can be configured for the second liquid channel, and a third address can be configured for the third liquid channel, so that the objects corresponding to the signals output by different pressure sensors 10 can be distinguished by the addresses.

[0086] Exemplarily, the integrated circuit bus interface is further configured to cascade the pressure detection device 171 with other pressure detection devices, so that more pressure sensors 10 can be used in parallel through cascading, thereby realizing pressure detection of more objects at the same time.

[0087] In addition, the pressure detection device 171 further includes a level conversion module 80, which is connected to the processing module 20 via an integrated circuit bus interface and is used to increase the second pressure signal to a preset voltage and then output it to the control device 172. For example, if the voltage signal corresponding to the pressure value is 3.3V, the voltage signal can be increased to 5V and output to the control device 172, thereby improving the stability of the transmission process.

[0088] It is understood that the structure shown in the embodiment of the present utility model does not constitute a specific limitation on the pressure detection device 171. In other embodiments of the present utility model, the pressure detection device 171 may include more or fewer components than shown in the figure, or combine some components, or split some components, or arrange the components differently. The components shown in the figure may be implemented in hardware, software, or a combination of software and hardware.

[0089] In addition, each of the above modules may include corresponding circuits or chips, etc. In this embodiment, the specific circuits of each of the above modules are not limited, as long as each module can achieve the corresponding functions. In addition, each module can be integrated on the same circuit substrate and arranged in the same housing, or integrated on different circuit substrates and arranged in different housings. The specific combination can be made according to the actual situation, and no specific limitation is made here.

[0090] In the embodiment of the utility model, since the nucleic acid sequencing system 100 is provided with a monitoring system 170, and the monitoring system 170 is configured to detect the liquid pressure when the reagent flows through the first liquid channel and / or the waste liquid flows through the second liquid channel, the liquid pressure detected by the monitoring system 170 can reflect the degree of blockage of the liquid channel, thereby achieving the purpose of detecting and troubleshooting the liquid channel. Furthermore, the monitoring system 170 can also generate a first prompt message when the liquid pressure is abnormal, so that not only can the user intuitively perceive whether an abnormality occurs in the liquid channel, but also the timeliness of abnormality perception can be improved.

[0091] In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, or the positions or positional relationships in which the disclosed product is usually placed when in use. They are only for the convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the electric vehicle or component referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0092] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0093] Finally, it should be noted that the above-described embodiments are only specific implementation methods of the utility model, which are used to illustrate the technical solution of the utility model, rather than to limit it. The protection scope of the utility model is not limited thereto. Although the utility model is described in detail with reference to the above-mentioned embodiments, ordinary technicians in this field should understand that any technician familiar with the technical field can still modify the technical solution recorded in the above-mentioned embodiments within the technical scope disclosed by the utility model, or can easily think of changes, or make equivalent replacements for some of the technical features therein; and these modifications, changes or replacements do not make the essence of the corresponding technical solution deviate from the spirit and scope of the technical solution of the embodiment of the utility model. They should all be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.

Claims

1. A nucleic acid sequencing system, characterized in that: include: Chips, reagent storage containers, waste liquid storage containers, diversion systems, detection systems, computer systems and monitoring systems; among which, One or more sequencing objects are attached to the chip; The reagent storage container is configured to store one or more reagents that can chemically react with the sequencing object; The waste liquid storage container is configured to store waste liquid after chemical reaction with the sequencing object; The fluid guiding system is configured to controllably transport the one or more reagents from the reagent storage container to the chip through the first liquid channel so as to contact with the sequencing object and undergo a chemical reaction, and controllably transport waste liquid after the chemical reaction from the chip to the waste liquid storage container through the second liquid channel; The detection system is configured to detect nucleic acid characteristics characterized by the sequencing object after chemical reaction with the one or more reagents; The computer system is configured to obtain nucleic acid feature information from the detection system, and identify the nucleic acid sequence of the sequencing object according to the nucleic acid feature information; The monitoring system is configured to detect the liquid pressure when the reagent flows through the first liquid channel and / or the waste liquid flows through the second liquid channel, and to provide a prompt for the detected liquid pressure.

2. The nucleic acid sequencing system according to claim 1, characterized in that: The fluid guiding system is further configured to controllably convey the one or more reagents from the reagent storage container to the waste liquid storage container through a third liquid channel; The monitoring system is also configured to detect the liquid pressure when the reagent flows through the third liquid channel, and to provide a prompt for the detected liquid pressure.

3. The nucleic acid sequencing system according to claim 2, characterized in that: The monitoring system is also configured to detect the gas pressure when gas is introduced into at least one of the first liquid channel, the second liquid channel and the third liquid channel, and to provide a prompt for the detected gas pressure.

4. The nucleic acid sequencing system according to claim 1, characterized in that: The monitoring system includes a pressure detection device and a control device, wherein the control device is configured to generate corresponding prompt information according to the pressure value detected by the pressure detection device, and can control the operation of the nucleic acid sequencing system based on the detected pressure value, wherein the pressure detection device includes: at least one pressure sensor, electrically connected to the processing module, configured to sense the liquid pressure when the reagent flows through the first liquid channel and / or the waste liquid flows through the second liquid channel, and output a first voltage to be measured representing a change in liquid pressure; A voltage conversion module, the input end of which is electrically connected to the external power supply, the output end of which is electrically connected to the processing module, and is configured to convert the input voltage provided by the external power supply into a reference voltage and then output it; The processing module is configured to determine and output a value of the liquid pressure sensed by the pressure sensor according to the first voltage to be measured and the reference voltage.

5. The nucleic acid sequencing system according to claim 4, characterized in that: The pressure detection device also includes: A temperature acquisition module, configured to acquire temperature data of the environment in which the pressure detection device is located, and generate a corresponding temperature signal according to the acquired temperature data; The processing module is further configured to compensate the value of the liquid pressure according to the temperature signal to obtain a compensated value of the liquid pressure.

6. The nucleic acid sequencing system according to claim 4, characterized in that: The processing module is also provided with a communication interface, which is configured to send the value of the liquid pressure to the control device; the communication interface includes at least one of an integrated circuit bus interface, a universal asynchronous receiver and transmitter interface, a serial communication interface and a controller area network interface.

7. The nucleic acid sequencing system according to claim 4, characterized in that: The pressure detection device further includes a data storage module, wherein the data storage module is configured to store calibration data for each of the pressure sensors, wherein the calibration data is configured to calibrate the value of the liquid pressure collected by the corresponding pressure sensor.

8. The nucleic acid sequencing system according to claim 4, characterized in that: The processing module further provides an address configuration interface, which is configured to output an object address of each of the pressure sensors, wherein the object addresses of different sensors are different so that different sensors can be distinguished by the object addresses.

9. The nucleic acid sequencing system according to claim 6, characterized in that: The pressure detection device further comprises a level conversion module; the communication interface comprises the integrated circuit bus interface; The level conversion module is connected to the processing module via the integrated circuit bus interface, and the level conversion module is configured to increase the value of the liquid pressure output by the processing module via the integrated circuit bus interface to a preset threshold value and then output it to the control device.

10. The nucleic acid sequencing system according to any one of claims 1 to 3, characterized in that: The monitoring system is also configured to prompt abnormal situations when the detected liquid pressure and / or gas pressure is abnormal.

11. The nucleic acid sequencing system according to claim 1, characterized in that: The monitoring system is also configured to collect and control the temperature of at least one of the chip and the reagent.

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