Adjustable PCR (Polymerase Chain Reaction) tube positioning adsorption device

Abstract

The utility model discloses an adjustable PCR (Polymerase Chain Reaction) tube positioning adsorption device, a support column comprises a bottom plate, a column body, a magnet and a cover body, the column body is arranged on the bottom plate and is provided with a middle cavity, the magnet is embedded in the middle cavity, the upper part of the side wall of the column body is provided with a vertical groove and a transverse groove which is positioned at the lower end of the vertical groove and is communicated with the vertical groove, and the vertical groove and the transverse groove form a positioning groove; the outer side of the column body is sleeved with a spring, the supporting frame is provided with a center hole and a plurality of positioning holes, elastic pieces are arranged in the positioning holes and used for transversely clamping PCR tubes, the elastic pieces are arranged on the side away from the column body, guide blocks are arranged on the side wall of the center hole, the column body is sleeved with the center hole, the guide blocks are embedded into the positioning grooves, and the lower end of the supporting frame abuts against the spring. The cover body is arranged at the upper end of the column body and the lower end abuts against the supporting frame. According to the utility model, the support frame can be adjusted up and down relative to the support column, so that the use requirements of PCR tubes with different sizes are met, the PCR tubes can be stably positioned and supported, and the adsorption stability of magnetic microspheres is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of magnetic microsphere separation devices, in particular to an adjustable PCR tube positioning adsorption device. Background Art

[0002] Magnetic microspheres, also known as magnetic beads, can be used for separation and purification by taking advantage of their ability to be easily adsorbed by magnets and separated from other substances. Specifically, the reaction tube (i.e., PCR tube) containing the magnetic bead solution is inserted into the magnetic rack. The magnetic rod provides lateral magnetic force to quickly gather the magnetic beads in the PCR tube, thereby separating them from other substances to achieve the purpose of separation and purification. It can be widely used in fields such as separating proteins and antibodies.

[0003] PCR tubes have different specifications, such as single tubes, linked tubes, etc., and different capacities such as 0.1ml and 0.2ml. For PCR tubes of different capacities, their heights and tube diameters are also different. In the prior art, in order to make the magnetic rack suitable for positioning PCR tubes of different capacity specifications, there is a magnetic rack for magnetic nano-microspheres with application number 202321429351.5, which discloses that a larger reaction tube can be inserted through a large mounting hole. When the reaction tube is smaller, the cylinder can be directly moved, and the connection block and the connection rack can be used to drive the cylinder to flip 180 degrees, so that the small mounting hole is facing upward. At this time, a smaller reaction tube can be inserted into it. The need to flip it not only leads to a complex structure, but also may cause the magnetic rack to be unable to be stably placed vertically due to instability. Utility Model Content

[0004] The purpose of the utility model is to provide an adjustable PCR tube positioning and adsorption device. Through the position of the guide block in the positioning groove and the action of the support frame and the spring, the support frame can be adjusted up and down relative to the support column to meet the use requirements of PCR tubes of different sizes. The PCR tube can be stably positioned and supported to ensure the stability of the magnetic microspheres during adsorption.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the utility model is: an adjustable PCR tube positioning and adsorption device, comprising a support column and a support frame, the support column comprising a bottom plate, a column, a magnet and a cover body, the column body is arranged on the bottom plate and has a middle cavity, the magnet is embedded in the middle cavity, the upper part of the side wall of the column body is provided with a vertical groove, and a horizontal groove located at the lower end of the vertical groove and connected to the vertical groove, the two form a positioning groove, the outer side of the column body is sleeved with a spring, the support frame is provided with a center hole and a plurality of positioning holes, the positioning hole is provided with an elastic member for horizontally clamping the PCR tube, the elastic member is arranged on a side away from the column body, the side wall of the center hole is provided with a guide block, the center hole is sleeved on the column body and the guide block is embedded in the positioning groove, the lower end of the support frame abuts against the spring, the cover body is arranged at the upper end of the column body and the lower end abuts against the support frame.

[0006] As a further optimization, a boss is provided on the column below the transverse groove, and the lower end of the spring abuts against the boss.

[0007] As a further optimization, the magnet includes two magnet units that are vertically adsorbed to each other, which can form a larger magnetic attraction effect in the middle and lower part of the magnet (where the two magnet units are adsorbed to each other).

[0008] As a further optimization, a plurality of the positioning holes are symmetrically arranged on the support frame, and the number is two or four, preferably four.

[0009] As a further optimization, the bottom plate is provided with a plurality of positioning grooves, the number of which matches the number of the positioning holes.

[0010] As a further optimization, the transverse groove is a 1 / 4 arc groove, which can achieve horizontal rotation of 90° after the support frame moves downward.

[0011] As a further optimization, the guide blocks are symmetrically provided with a pair of horizontal cylinders, and the pair of positioning grooves are axially symmetrically arranged to ensure the balance during the downward movement of the support frame and when the guide blocks are located in the transverse groove and supported upward by the spring.

[0012] As a further optimization, a chamfer is provided at the connection between the transverse groove and the vertical groove to facilitate the guide block to slide from the vertical groove into the transverse groove.

[0013] As a further optimization, the elastic member is an elastic gasket, and the elastic gasket is arranged on the inner wall of the positioning hole.

[0014] As a further optimization, the elastic member includes a spring and an elastic gasket, one end of the spring is arranged on the inner wall of the positioning hole, and the elastic gasket is arranged on the other end of the spring.

[0015] Compared with the prior art, the utility model has the following beneficial effects:

[0016] Through the position of the guide block in the positioning groove (in the vertical groove or the horizontal groove) and the action of the support frame and the spring, the support frame can be adjusted up and down relative to the support column to meet the use requirements of PCR tubes of different sizes. The PCR tubes can be stably positioned and supported to ensure the stability of the magnetic microspheres during adsorption. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural diagram of the utility model.

[0018] Figure 2 This is a structural diagram of the support column of the utility model.

[0019] Figure 3 It is a structural diagram of the support frame of the utility model.

[0020] Figure 4 It is a structural diagram of the cover body of the utility model.

[0021] Figure 5 This is a structural diagram of the elastic member of the utility model being arranged on a support frame.

[0022] Figure 6 This is a structural diagram of the utility model in another state. DETAILED DESCRIPTION

[0023] The following are specific embodiments of the present invention and the accompanying drawings to further describe the technical solution of the present invention, but the present invention is not limited to these embodiments.

[0024] like Figures 1 to 5As shown, an adjustable PCR tube positioning adsorption device comprises a support column 1 and a support frame 2. The support column 1 comprises a bottom plate 11, a column 12, a cover 14 and a magnet 15. The column 12 is fixedly formed on the bottom plate 11 and has a middle cavity 120. The magnet 15 is embedded in the middle cavity 120. The upper end of the magnet 14 leaves a clearance hole 120' in the middle cavity 120 to facilitate the installation of the cover 14 on the column 12. The upper part of the side wall of the column 12 is provided with a vertical groove 121, and a horizontal groove 122 located at the lower end of the vertical groove 121 and connected to the vertical groove 121, and the two form a The positioning groove 12a of the L-shaped structure, the outer side of the column 12 is sleeved with a spring 13, the support frame 2 is provided with a center hole 21 and four symmetrically arranged positioning holes 22, the positioning hole 22 is provided with an elastic member 221 for horizontally clamping the PCR tube, the elastic member 221 is arranged on the side away from the column 12, the side wall of the center hole 21 is provided with a guide block 211, the center hole 21 is sleeved on the column 12 and the guide block 211 is embedded in the positioning groove 12a, the lower end of the support frame 2 is in contact with the spring 13, the cover body 14 is arranged at the upper end of the column 12 and the lower end is in contact with the support frame 2.

[0025] In the present invention, four positioning holes 22 are symmetrically arranged on the support frame 2, so that the magnet 15 in the column 12 can act on four PCR tubes at the same time, thereby improving the adsorption effect on the magnetic microspheres; for larger PCR tubes, when they are positioned by the support frame 2, specific reference can be made to Figure 1 As shown, the spring 13 pushes the support frame 2 upward through its action and makes the upper end of the support frame 2 abut against the cover body 14 (at this time, the guide block 211 is located at the top of the vertical groove 121), that is, the support frame 2 is located at a high position, and the support function of the bottom plate 11 can meet the positioning of the upper part of the larger PCR tube and the support of the bottom, refer to Figure 6 As shown, when the application object is a smaller PCR tube, by pressing the support frame 2 downward, the guide block 211 moves downward along the vertical groove 121 and compresses the spring 13. When the guide block 211 moves to the bottom of the vertical groove 121, the support frame 2 is rotated to make the guide block 211 enter the transverse groove 122. Since the guide block 211 is limited in the transverse groove 122, the spring 13 cannot lift the support frame 2. At this time, the support frame 2 is in a low position, and the smaller PCR tube can be placed in the positioning hole 22. The tube wall of the smaller PCR tube can be positioned by the elastic member 221, so the upper and lower parts of the smaller PCR tube are supported and positioned by the support frame 2 and the bottom plate 11. Through the above-mentioned action, the position of the support frame 2 (positioning hole 22) can be adjusted easily and quickly, and the magnetic field position close to the magnet 15 can be achieved for PCR tubes of different sizes, so as to realize efficient magnetic attraction of the magnetic microspheres in the PCR tube.

[0026] For the assembly of the support column 1 and the support frame 2, the magnet 15 is first placed in the middle cavity 120, the spring 13 is sleeved on the support column 1, and then the guide block 211 on the support frame 2 is inserted into the vertical groove 121 so that the support frame 2 is sleeved on the support column 1 through its center hole 21, and the lower end of the support frame 2 abuts against the spring 13, and then the cover body 14 is installed on the support column 1, and the lower end can abut against the upper end of the support frame 2. Furthermore, the specific structure of the cover body 14 includes an upper cover plate 141, and a bottom plate 142 formed at the lower end of the upper cover plate 141, the bottom plate 142 is embedded in the clearance hole 120' to seal the middle cavity 120, and the side of the upper cover plate 141 protrudes from the outer wall of the column 11 to limit the abutment against the upper end of the support frame 2.

[0027] In the utility model, the position of the guide block 211 in the positioning groove 2a and the action of the support frame 2 and the spring 13 enable the support frame 2 to be adjusted up and down relative to the support column 1, so as to meet the use requirements of PCR tubes of different sizes, stably position and support the PCR tubes, and ensure the stability of the magnetic microspheres during adsorption.

[0028] In addition, a boss 12b may be provided on the column 11 below the transverse groove 122, and the lower end of the spring 13 abuts against the boss 12b, so that the spring 13 has a smaller stroke when abutting against the lower end of the support frame 2, and can fully exert its elastic effect.

[0029] Furthermore, the magnet 13 includes two magnet monomers that are vertically adsorbed to each other, and preferably, a pair of magnet monomers are adsorbed to each other at the middle and lower part of the column 11. The design closer to the bottom of the column 11 can be more conducive to the adsorption and fixation of magnetic microspheres in smaller PCR tubes. This design will not affect the adsorption of magnetic microspheres in larger PCR tubes.

[0030] For example Figure 2 As shown, four positioning grooves 111 can be provided on the bottom plate 11, and the bottom end of the PCR tube can be embedded therein to ensure placement stability.

[0031] Based on the setting of the four positioning grooves 111, the transverse groove 121 can be set as a 1 / 4 arc groove, that is, after the guide block 211 enters the transverse groove 122 from the vertical groove 121, it drives the positioning hole 22 to rotate 90° in the horizontal plane, so that the positioning hole 22 can still be set relative to the positioning groove 111.

[0032] Preferably, the guide block 211 is symmetrically provided with a pair of horizontal cylinders, which is conducive to its movement in the positioning groove 12a; the pair of positioning grooves 12a are axially symmetrically arranged, so that the opposite sides of the column 11 can act on the opposite sides of the center hole 21 respectively, ensuring the stability and balance of the support frame 2 when rotating and supporting in the plane where the transverse groove 122 is located, thereby ensuring that the support frame 2 is in a horizontal state.

[0033] The connection between the transverse groove 121 and the vertical groove 122 is provided with an arc-shaped chamfer, so as to facilitate the guide block 211 to change the moving direction smoothly.

[0034] For the elastic member 221 that can realize lateral support and positioning of the PCR tube, it can adopt the conventional structure for lateral support clamping in the prior art. For example, in one embodiment, the elastic member 221 is an elastic gasket, and the elastic gasket is arranged on the inner wall of the positioning hole 22. The elastic gasket needs to have a certain thickness and excellent elasticity to ensure that it can support smaller PCR tubes. It has a simple structure; in another embodiment, the elastic member 221 can be selected in the form of a combination of a spring and an elastic gasket, one end of the spring is arranged on the inner wall of the positioning hole 22, and the elastic gasket is arranged at the other end of the spring. The supportable and compressible space is realized by the spring. In this embodiment, the elastic gasket can be a thinner structure.

[0035] The specific embodiments described herein are merely examples of the spirit of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in similar ways, but they will not deviate from the spirit of the present invention or exceed the scope defined by the appended claims.

Claims

1. An adjustable PCR tube positioning adsorption device, characterized in that: It includes a support column and a support frame, the support column includes a base plate, a column, a magnet and a cover body, the column body is arranged on the base plate and has a middle cavity, the magnet is embedded in the middle cavity, the upper part of the side wall of the column body is provided with a vertical groove, and a horizontal groove located at the lower end of the vertical groove and connected with the vertical groove, the two form a positioning groove, the outer side of the column body is sleeved with a spring, the support frame is provided with a center hole and a plurality of positioning holes, the positioning hole is provided with an elastic member for horizontally clamping the PCR tube, the elastic member is arranged on the side away from the column body, the side wall of the center hole is provided with a guide block, the center hole is sleeved on the column body and the guide block is embedded in the positioning groove, the lower end of the support frame abuts against the spring, the cover body is arranged at the upper end of the column body and the lower end abuts against the support frame.

2. The adjustable PCR tube positioning adsorption device according to claim 1, characterized in that: A boss is provided on the column below the transverse groove, and the lower end of the spring abuts against the boss.

3. The adjustable PCR tube positioning adsorption device according to claim 1, characterized in that: The magnet comprises two magnet monomers which are vertically attracted to each other.

4. The adjustable PCR tube positioning adsorption device according to claim 1, characterized in that: The plurality of positioning holes are symmetrically arranged on the support frame, and the number of the positioning holes is two or four.

5. The adjustable PCR tube positioning adsorption device according to claim 1 or 4, characterized in that: The bottom plate is provided with a plurality of positioning grooves.

6. The adjustable PCR tube positioning adsorption device according to claim 1 or 4, characterized in that: The transverse groove is a 1 / 4 arc groove.

7. The adjustable PCR tube positioning adsorption device according to claim 1, characterized in that: The guide blocks are symmetrically arranged in a pair and are horizontal cylindrical bodies, and the pair of positioning grooves are axially symmetrically arranged.

8. The adjustable PCR tube positioning adsorption device according to claim 1 or 7, characterized in that: A chamfer is provided at the connection between the transverse groove and the vertical groove.

9. The adjustable PCR tube positioning adsorption device according to claim 1, characterized in that: The elastic member is an elastic gasket, and the elastic gasket is arranged on the inner wall of the positioning hole.

10. The adjustable PCR tube positioning adsorption device according to claim 1, characterized in that: The elastic member comprises a spring and an elastic gasket, one end of the spring is arranged on the inner wall of the positioning hole, and the elastic gasket is arranged on the other end of the spring.

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