Integrated sampling and culturing centrifugal tube

Abstract

The utility model discloses an integrated sampling culture centrifuge tube which comprises a tube body and a tube opening, a telescopic suction tube is communicated between the tube body and the tube opening, an exhaust sampling cover is detachably mounted on the tube opening, and an exhaust plug and a sampling suction head which are communicated with the tube body are arranged on the exhaust sampling cover. According to the integrated sampling and culturing centrifugal tube, the telescopic suction tube structure is adopted, gas in the tube body can be exhausted from the exhaust plug when the telescopic suction tube is compressed, negative pressure is generated in a bottle by stretching the telescopic suction tube, and a biological sample can be directly sucked into the tube body through the sampling suction head; the sampling operation can be completed without using a transfer device such as an injector; besides, the exhaust sampling cover can be taken down to add a culture medium and the like into the tube, culture is directly carried out in the tube, the tube body is similar to an existing centrifugal tube in structure, the tube body can be directly placed on a centrifugal machine to carry out centrifugal operation, sample transfer is reduced, and pollution to the sample can be reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of centrifugal devices, in particular to an integrated sampling and culturing centrifuge tube. Background Art

[0002] Culture tubes are mainly used for the cultivation of biological materials such as bacteria, viruses, blood, and tissues. First, the biological material needs to be sampled using a syringe, etc., and then transferred to a culture tube containing a culture medium for cultivation. After the cultivation is completed, it is transferred to a centrifuge tube by a pipette, etc. for centrifugal operation. In this process, the biological material has undergone multiple transfers, which increases the possibility of contamination of the biological material. The prior art with patent number CN205893260U discloses a centrifugal culture bottle, which is provided with an exhaust device on the side wall of the bottle body, which can generate negative pressure in the bottle so as to directly extract the biological material sample, which can reduce the intermediate links, but the exhaust device is located on the side wall of the bottle body. If an existing centrifuge is used, it will hinder the centrifugal operation and fail to achieve the centrifugal purpose. Summary of the invention

[0003] The utility model aims to solve at least one of the above-mentioned technical problems and provides an integrated sampling and culturing centrifuge tube, which integrates sampling, culturing and centrifugal operations to reduce sample transfer.

[0004] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0005] An integrated sampling and culturing centrifuge tube comprises a tube body and a tube mouth, wherein a telescopic suction tube is connected between the tube body and the tube mouth, an exhaust sampling cover is detachably mounted on the tube mouth, and the exhaust sampling cover is provided with an exhaust plug connected to the tube body and a sampling suction head.

[0006] As an improvement of the above technical solution, the telescopic suction pipe includes a bellows, and the bellows is connected between the pipe body and the pipe opening.

[0007] As an improvement of the above technical solution, convex rings are provided on the outer walls of both ends of the bellows, and a telescopic limit buckle is detachably installed on the outer wall of the bellows and at a position between the two groups of convex rings.

[0008] As an improvement of the above technical solution, a plug hole is provided on the exhaust sampling cover, a first exhaust hole connecting the plug hole and the tube body is provided on the exhaust sampling cover, the exhaust plug can be rotatably installed in the plug hole, and a second exhaust hole connected to the first exhaust hole is provided on the exhaust plug.

[0009] As an improvement of the above technical solution, a limiting ring groove is provided on the exhaust sampling cover and at the bottom of the plug hole, and a limiting ring that can cooperate with the limiting ring groove is provided on the bottom of the exhaust plug.

[0010] As an improvement of the above technical solution, an external thread is provided on the outer periphery of the pipe mouth, and an internal thread that can cooperate with the external thread is provided on the inner wall of the exhaust sampling cover.

[0011] As an improvement of the above technical solution, it also includes a centrifugal cover, which can be detachably installed on the pipe mouth.

[0012] As an improvement of the above technical solution, the bottom of the tube body is configured in a conical shape.

[0013] As an improvement of the above technical solution, scale markings are provided on the outer surface of the tube body.

[0014] Compared with the prior art, the beneficial effects of this application are:

[0015] The utility model discloses an integrated sampling and culturing centrifuge tube, which adopts a telescopic suction tube structure. When the telescopic suction tube is compressed, the gas in the tube body can be discharged from the exhaust plug. The telescopic suction tube is then stretched to generate negative pressure in the bottle, so that the biological sample can be directly sucked into the interior of the tube body through the sampling suction head, and the sampling operation can be completed without using a transfer device such as a syringe. In addition, the exhaust sampling cover can be removed to add culture medium to the tube, and culture can be carried out directly in the tube. The tube body is similar to the existing centrifuge tube structure and can be directly placed on a centrifuge for centrifugal operation, which reduces the transfer of samples and can reduce the contamination to the samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The specific implementation of the utility model is further described in detail below with reference to the accompanying drawings, wherein:

[0017] Figure 1 It is a schematic diagram of the structure of an embodiment of the utility model;

[0018] Figure 2 This is a schematic diagram of the explosion structure of an embodiment of the utility model;

[0019] Figure 3 A cross-sectional view of an embodiment of the utility model;

[0020] Figure 4 It is a cross-sectional view of the exhaust sampling cover in the embodiment of the utility model;

[0021] Figure 5 It is a top view of the exhaust sampling cover in the embodiment of the utility model;

[0022] Figure 6 It is a cross-sectional view of the telescopic limiting buckle in the embodiment of the utility model. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] It should be noted that when a component is referred to as being "fixed to" another component, it may be directly on the other component or there may be a central component. When a component is considered to be "connected to" another component, it may be directly connected to the other component or there may be a central component at the same time. When a component is considered to be "set on" another component, it may be directly set on the other component or there may be a central component at the same time. When a component is referred to as being "set in the middle", it does not only mean being set in the middle, as long as it is not set at both ends within the range defined by the middle. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0026] like Figures 1 to 6 As shown, the utility model provides an integrated sampling and culture centrifuge tube, including a tube body 10 and a tube mouth 11, wherein a telescopic suction tube 20 is connected between the tube body 10 and the tube mouth 11, and an exhaust sampling cover 30 can be detachably installed on the tube mouth 11, and an exhaust plug 31 and a sampling suction head 32 connected to the tube body 10 are provided on the exhaust sampling cover 30. Among them, the bottom of the tube body 10 is set in a conical shape, that is, the shape of the tube body 10 is the same as that of the existing centrifuge tube, which is not only convenient for placement on the existing centrifuge for use, but also can improve the centrifugal effect, and can also be set to different capacity sizes according to needs; in addition, a scale mark 13 is provided on the outer surface of the tube body 10 for marking the capacity. The sampling suction head 32 can be connected by a hose so as to extend into the container of the biological sample to absorb the sample; and a one-way liquid inlet valve is provided in the sampling suction head 32 to prevent the sample inside the tube body 10 from flowing back.

[0027] See also Figure 2 and Figure 3In a specific embodiment of the present application, the telescopic suction pipe 20 includes a bellows 21, and the bellows 21 is connected between the tube body 10 and the pipe mouth 11. The bellows 21 is a corrugated hose that can be repeatedly compressed and stretched, and can be made of plastic or rubber and other materials. It is a prior art and will not be described in detail in this application; the bellows 21 can achieve a sealed telescopic connection to control the air pressure inside the tube body 10. During operation, the tube body 10 and the pipe mouth 11 can be pinched and grasped respectively, and the bellows 21 can be moved up and down to compress or stretch. In other embodiments, the telescopic suction pipe 20 can also adopt a multi-section telescopic pipe structure.

[0028] Furthermore, since centrifugal operation of culture is required after sampling, in order to prevent the bellows 21 from being compressed due to accidental touch, convex rings 22 are provided on the outer walls of both ends of the bellows 21, and a telescopic limit buckle 23 is detachably mounted on the outer wall of the bellows 21 and located between the two groups of convex rings 22. The telescopic limit buckle 23 is a semicircular ring buckle, and its specific structure is as follows: Figure 6 As shown; when sampling operation is not required, the telescopic limit buckle 23 can be mounted on the outer periphery of the bellows 21 so that its two ends can be against the two sets of convex rings 22 to prevent the bellows 21 from being compressed and deformed, while protecting the outer periphery of the bellows 21.

[0029] See also Figures 3 to 5 In a specific embodiment of the present application, in order to facilitate the adjustment of the connection state of the exhaust, the exhaust sampling cover 30 is provided with a plug hole 33, the exhaust sampling cover 30 is provided with a first exhaust hole 34 connecting the plug hole 33 and the tube body 10, the exhaust plug 31 is rotatably installed in the plug hole 33, and the exhaust plug 31 is provided with a second exhaust hole 35 that can be connected to the first exhaust hole 34. The exhaust plug 31 can be rotated to make the second exhaust hole 35 connected to or staggered with the first exhaust hole 34, that is, the connection state of the exhaust can be adjusted by rotation. When performing the sampling operation, first rotate the exhaust plug 31 to a position where the second exhaust hole 35 is aligned with the first exhaust hole 34, and then compress the bellows 21 to exhaust the gas. After the bellows 21 is in a compressed state, rotate the exhaust plug 31 to make the second exhaust hole 35 misaligned with the first exhaust hole 34, that is, in a blocked state, and then push the sampling pipette head 32 deep into the sample container. Then stretch the bellows 21 to increase the space inside the tube, generate negative pressure attraction, and suck the sample into the tube body 10 through the sampling pipette head 32 to complete the sampling operation.

[0030] Furthermore, in order to prevent the exhaust plug 31 from being pushed open by air pressure when the bellows 21 is compressed, a limiting ring groove 36 is provided on the exhaust sampling cover 30 and at the bottom of the plug hole 33, and a limiting ring 37 that can cooperate with the limiting ring groove 36 is provided on the bottom of the exhaust plug 31.

[0031] In order to facilitate the removal of the exhaust sampling cover 30 for operations such as replacing the cover or adding culture medium, an external thread 12 is provided on the outer periphery of the tube mouth 11, and an internal thread 38 that can cooperate with the external thread 12 is provided on the inner wall of the exhaust sampling cover 30. A sealing ring can also be arranged inside the exhaust sampling cover 30 to enhance the sealing performance. In another specific embodiment of the present application, since the sampling head 32 is relatively long and has an impact on centrifugation, in order to facilitate the centrifugation operation, a centrifugal cover 40 is also included. The centrifugal cover 40 can be detachably installed on the tube mouth 11. The centrifugal cover 40 can adopt an existing centrifugal tube cover. After the sampling is completed, the exhaust sampling cover 30 can be removed and the centrifugal cover 40 can be replaced; specifically, the centrifugal cover 40 is provided with a small hole to facilitate the sample suction needle to suck the sample when the machine is installed, and a bacterial filter membrane is provided inside the cover to prevent contamination from external air during the culture process. In addition, when cell culture is performed, the centrifuge tube can be used to directly harvest cells using a fully automatic cell harvester after culture. Using the centrifuge tube for blood sample inoculation, cell culture and machine cell harvesting can reduce many intermediate links, prevent cell contamination, ensure artificial biosafety, be simple and economical, and have practical application value.

[0032] The utility model discloses an integrated sampling and culturing centrifuge tube, which adopts a telescopic suction tube 20 structure. When the telescopic suction tube 20 is compressed, the gas in the tube body can be discharged from the exhaust plug 31. Then, the telescopic suction tube 20 is stretched to generate negative pressure in the bottle, so that the biological sample can be directly sucked into the tube body 10 through the sampling suction head 32, and the sampling operation can be completed without using a transfer device such as a syringe. In addition, the exhaust sampling cover 30 can also be removed to add culture medium to the tube, and culture can be carried out directly in the tube. The tube body is similar to the existing centrifuge tube structure and can be directly placed on a centrifuge for centrifugal operation, which reduces the transfer of samples and can reduce the contamination of the samples.

[0033] The above embodiments are only used to illustrate the technical solution of the utility model but not to limit it. Any modification or equivalent substitution that does not deviate from the spirit and scope of the utility model shall be included in the scope of the technical solution of the utility model.

Claims

1. An integrated sampling and culture centrifuge tube, characterized in that: The invention comprises a tube body and a tube mouth, wherein a telescopic suction tube is connected between the tube body and the tube mouth, an exhaust sampling cover is detachably mounted on the tube mouth, and an exhaust plug connected to the tube body and a sampling suction head are arranged on the exhaust sampling cover.

2. The integrated sampling and culturing centrifuge tube according to claim 1, characterized in that: The telescopic suction pipe comprises a bellows, and the bellows is connected between the pipe body and the pipe opening.

3. The integrated sampling and culturing centrifuge tube according to claim 2, characterized in that: The outer walls at both ends of the bellows are provided with convex rings, and a telescopic limit buckle is detachably mounted on the outer wall of the bellows and located between the two groups of convex rings.

4. The integrated sampling and culturing centrifuge tube according to claim 1, characterized in that: The exhaust sampling cover is provided with a plug hole, the exhaust sampling cover is provided with a first exhaust hole connecting the plug hole and the tube body, the exhaust plug can be rotatably installed in the plug hole, and the exhaust plug is provided with a second exhaust hole that can be connected to the first exhaust hole.

5. The integrated sampling and culturing centrifuge tube according to claim 4, characterized in that: A limiting ring groove is provided on the exhaust sampling cover at the bottom of the plug hole, and a limiting ring capable of cooperating with the limiting ring groove is provided on the bottom of the exhaust plug.

6. The integrated sampling and culturing centrifuge tube according to claim 1, characterized in that: An external thread is arranged on the outer periphery of the pipe opening, and an internal thread which can cooperate with the external thread is arranged on the inner wall of the exhaust gas sampling cover.

7. The integrated sampling and culturing centrifuge tube according to claim 1, characterized in that: Also included is a centrifugal cover which can be detachably mounted on the nozzle.

8. The integrated sampling and culturing centrifuge tube according to claim 1, characterized in that: The bottom of the tube body is arranged in a conical shape.

9. The integrated sampling and culturing centrifuge tube according to claim 1, characterized in that: The outer surface of the tube body is provided with scale marks.

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