Whole-membrane multi-antibody incubation box for Western Blot

Abstract

The utility model relates to a whole membrane multi-antibody incubation box used for Western Blot. A box cover is rotatably arranged on a box body, a plurality of partition plates are arranged in the box body, the inner space of the box body is divided into a plurality of cells through the partition plates, the left ends and the right ends of the partition plates are lightly pressed on the two sides of the upper surface of the box body, and the partition plates can slide on the upper surface of the box body. Scale marks are arranged at the bottom of the box body, so that the partition plates can partition neatly. Liquid-leakage-proof sealing silica gel strips are arranged on the upper surface of the box body and the periphery of the partition plate, so that the sealing performance of each cell is ensured, and antibody leakage is avoided; a cover-in-cover is rotatably arranged in the middle of the box cover. The partition plate is limited through the box cover and the sealing silica gel strip, so that the partition plate does not incline laterally; the cover-in-cover is arranged, so that the antibody is not interfered by external factors, and the incubation condition of the antibody can be clearly observed. Operation is easy and convenient, the application range is wide, exquisite and convenient, and cost can be saved.

Description

Technical Field

[0001] The utility model relates to experimental equipment for biomedical research, in particular to a whole-membrane multi-antibody incubation box for Western Blot. Background Art

[0002] Western Blot is a technique that combines high-resolution gel electrophoresis with immunochemical analysis and is commonly used in molecular biology, biochemistry, and immunogenetics research. This technique determines the expression level of a specific protein in a sample by transferring the protein from the gel to a membrane and then using specific antibodies to detect the treated cells or biological tissue samples. The analysis of the staining location and depth helps to obtain more accurate results.

[0003] In Western Blot experiments, antibody incubation is a relatively important step. Only under appropriate humidity and temperature conditions can the binding between antibodies and antigens and between the two antibodies reach the best state. The internal reference protein is a common stable expression protein in cells. The consistency of its expression level can ensure that the protein loading amount of samples in each lane is the same. Therefore, in Western Blot, the selection of the internal reference protein is crucial for accurately quantifying the relative expression level of the target protein.

[0004] Polyvinylidene fluoride (PVDF) is a solid support commonly used in Western Blot experiments. PVDF membranes are hydrophobic, and the membrane pore size varies. As the membrane pore size continues to decrease, the membrane binds to low molecular weight proteins more firmly. In traditional Western Blot experiments, due to the specificity of antibodies, it is often necessary to incubate the entire membrane containing the target protein and the internal reference protein with two different primary antibodies, or cut the entire membrane to incubate different primary antibodies separately, and then splice. These steps not only increase the time cost, but also the membrane cutting and splicing operations are easily abused, affecting the credibility and repeatability of the experimental data, and may even lead to academic misconduct. In addition, different primary antibodies may correspond to different resistances, so the secondary antibodies also need to be incubated separately. The currently commonly used antibody incubation box cannot meet the needs of incubating multiple antibodies on the same membrane at the same time.

[0005] Based on the above needs, the present invention provides a whole membrane multi-antibody incubation box for Western Blot, which aims to effectively solve the problems caused by membrane cutting and splicing from the source. The incubation box has a reasonable design, is easy to operate, can accommodate multiple antibodies at the same time, and realizes the incubation of multiple antibodies on the same membrane, thereby reducing operational errors and simplifying the experimental process. Summary of the invention

[0006] The utility model aims to provide a whole membrane multi-antibody incubation box for Western Blot, so as to solve the problems caused by membrane cutting and splicing in traditional experiments, and improve the credibility and repeatability of experimental data. The incubation box is reasonably designed to make the experimental operation simpler, and can accommodate multiple antibodies at the same time, so as to realize the incubation of multiple antibodies on the same membrane.

[0007] In order to achieve the above-mentioned expected technical optimization effects, the specific solutions are as follows:

[0008] The utility model comprises a box body, a box cover and a partition. The box cover can be rotatably arranged on the box body, and the box body is provided with a plurality of partitions, through which the inner space of the box body is divided into a plurality of slots, and the left and right ends of the partition are lightly pressed on both sides of the upper surface of the box body and can slide on the upper surface of the box body. The bottom of the box body is provided with scale lines, so that the partitions can be neatly divided.

[0009] The upper surface of the box body and the surrounding of the partition are all provided with anti-leakage liquid sealing silicone strips to ensure the sealing of each slot and prevent the leakage of antibodies; the middle part of the box cover is rotatably provided with a cover in the cover.

[0010] The partition is limited by the box cover and the sealing silicone strip to prevent it from tilting; a cover within a cover is provided to ensure that the antibody is not interfered by external factors and the antibody incubation status can be clearly observed.

[0011] Furthermore, the box body and the box cover are riveted on one side, and the other side is provided with a buckle and a fixing protrusion cooperating with the buckle.

[0012] Furthermore, the cover in the cover can be rotatably fixed in the middle of the box cover by matching the mounting shaft with a hole arranged on the box cover.

[0013] Furthermore, the cover in the cover is made of transparent material.

[0014] Furthermore, a limiting groove is arranged on the cover-in-cover, and a limiting block is arranged on the box cover; when the cover-in-cover is closed, the limiting groove and the limiting block fit together to limit the cover-in-cover.

[0015] Furthermore, the cover-in-cover is also provided with a handle.

[0016] Compared with the prior art, the utility model has the following technical optimization effects:

[0017] 1. Easy operation: The utility model is simple and easy to use, avoiding the cutting of PVDF membrane, greatly simplifying the experimental steps and reducing the risk of mistakes;

[0018] 2. Wide range of applications: Contains a movable baffle, which can be adjusted according to experimental needs to meet the simultaneous antibody incubation of proteins with different molecular weights, and is highly practical;

[0019] 3. Exquisite and convenient: small and easy to assemble, suitable for small laboratories, small footprint, inexpensive and good quality;

[0020] 4. Cost saving: The antibody incubation box partition divides the space into small slots, reducing the amount of reagents used and saving costs;

[0021] 5. Integrate multiple specific antibodies on the same PVDF membrane, covering the detection of multiple target proteins at one time, effectively reducing the possibility of experimental data being tampered with. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the overall structure of the utility model in a closed state;

[0023] Figure 2 This is a schematic diagram of the overall structure of the utility model in an open state;

[0024] Figure 3 for Figure 1 A schematic diagram of the structure of the middle box;

[0025] Figure 4 for Figure 1 Schematic diagram of the structure of the middle box cover;

[0026] Figure 5 for Figure 4 Schematic diagram of the structure without the cover in the cover;

[0027] Figure 6 for Figure 2 Schematic diagram of the structure of the middle partition;

[0028] Figure 7 This is a schematic diagram of the structure of the cover in the cover open state;

[0029] Figure 8 It is a schematic diagram of the structure of the box cover and the cover in the cover when they are opened;

[0030] Fig. 9 It is a schematic diagram of the structure of the cover in the cover. DETAILED DESCRIPTION

[0031] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. In the description of the present invention, it should be noted that the terms "middle", "upper", "lower", "left", "right", "inner", "outer", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the present invention and simplifying the description. For those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scopes. In summary, the content of this specification should not be understood as limiting the present invention.

[0032] The features and advantages of the present invention will be further described in detail below with reference to the accompanying drawings.

[0033] like Figure 1 and 2 As shown, a whole membrane multi-antibody incubation box for Western Blot comprises a box cover 1, a box body 2 and a partition 3. The box cover 1 is rotatably arranged on the box body 2 by a buckle, and the box body 2 is divided by a plurality of partitions 3.

[0034] like Figure 3 As shown, the three side walls of the box body 2 are all provided with fixing protrusions 21 that cooperate with the buckles, a sealing silicone strip 22 is provided on the opening side of the box body, and a scale line 23 is provided on the bottom surface of the box body.

[0035] like Figure 4 As shown, the three side walls of the box cover 1 are provided with buckles 111, each buckle cooperates with a fixed protrusion on the box body to fix the box cover 1 on the box body, and at the same time, multiple partitions 3 in the box body 2 are firmly fixed between the box body sealing silicone strip 22 and the whole membrane multi-antibody incubation box fixed cover 1, ensuring that the partitions are firmly fixed and not tilted.

[0036] When the partition is inserted into the box body, the left and right positions of the partition can be efficiently positioned through the internal scale lines 23 to prevent the partition from tilting, effectively improving the accuracy and stability of antibody incubation and significantly increasing experimental efficiency.

[0037] The box cover 1 and the box body 2 are not provided with buckles or riveted on the side of the fixing protrusion 21 .

[0038] like Figure 5 As shown, the partition 3 is provided with a partition support protrusion 31 and a partition sealing silicone strip 32. The partition 3 and the contact surface of the box body are both installed with a partition sealing silicone strip 32 to ensure that the partition 3 has a better separation and sealing effect after being inserted into the box body 2.

[0039] like Figures 6 to 8 As shown, the middle of the box cover 1 is provided with a cover-in-cover 12. Fig. 9 As shown, the cover-in-cover 12 can be rotatably fixed in the middle of the box cover 1 by matching the mounting shaft 121 with the hole 112 provided on the box cover; the cover-in-cover 12 is provided with a limiting groove 122 which matches the limiting block 113 provided on the box cover 1.

[0040] The cover-in-cover 12 is also provided with a handle 123 .

[0041] When the cover-in-cover 12 is closed, the limiting groove 122 fits with the limiting block 113 to prevent the cover-in-cover 12 from falling into the box body 2 when the partition 3 is less.

[0042] In this embodiment, the cover in the cover is made of transparent material.

[0043] The incubation box is used as follows:

[0044] Place the PVDF membrane containing the protein on the bottom surface of the box body 2, and the inner wall of the box body 2 is completely fitted with the edge of the PVDF membrane; take the corresponding number of partitions 3 according to the experimental requirements, align the partitions 3 with the internal scale lines 23 and insert the partitions 3 into the box body 2 to achieve regional separation in the PVDF membrane placement area; after the partitions 3 are placed, cover the box cover 1 with force, buckle the fixed cover buckle 111 on the fixed protrusion 21, and after the three sides are buckled, the partition 3 is firmly fixed in the specified position. Grasp the handle 123 by hand and rotate the cover 12 along the installation axis to open, add the antibodies required for the experiment to the corresponding grid according to the experimental plan for antibody incubation; cover the cover 12 and observe the antibody incubation situation through the cover 12, accurately observe the experimental related information and progress, and facilitate subsequent experimental operations. When using the whole membrane multi-antibody incubation box described in the utility model for antibody incubation, the PVDF membrane is in a complete state throughout the whole process, and there is no need to cut and split it, which significantly simplifies the experimental steps and avoids mistakes caused by improper cutting.

[0045] The application of the technical principle and implementation method is explained in this utility model. The above description is only for understanding and recognition of the present invention. In addition, based on the idea of ​​the present invention, technical personnel in this field can make various deformations and modifications in the specific implementation method and application scope, but these deformations and modifications should all fall within the protection scope of the present invention.

Claims

1. A whole-membrane multi-antibody incubation box for Western Blot, comprising a box body, a box cover and a partition, characterized in that: The box cover can be rotatably arranged on the box body. The box body is provided with a plurality of partitions, through which the internal space of the box body is divided into a plurality of slots. The left and right ends of the partitions are lightly pressed on both sides of the upper surface of the box body and can slide on the upper surface of the box body. The bottom of the box body is provided with scale lines to facilitate neat partitioning. The upper surface of the box body and the surrounding of the partition are all provided with anti-leakage liquid sealing silicone strips to ensure the sealing of each slot and prevent the leakage of antibodies; the middle part of the box cover is rotatably provided with a cover in the cover.

2. The whole membrane multi-antibody incubation kit for Western Blot according to claim 1, characterized in that: The box body and the box cover are riveted on one side, and the other side is provided with a buckle and a fixing protrusion matched with the buckle.

3. The whole membrane multi-antibody incubation kit for Western Blot according to claim 1, characterized in that: The cover in the cover is matched with a mounting shaft and a hole arranged on the box cover, and can be rotatably fixed in the middle of the box cover.

4. The whole membrane multi-antibody incubation kit for Western Blot according to claim 1, characterized in that: The cover in the cover is made of transparent material.

5. The whole membrane multi-antibody incubation kit for Western Blot according to claim 1, characterized in that: The cover-in-cover is provided with a limiting groove, and the box cover is provided with a limiting block; when the cover-in-cover is closed, the limiting groove and the limiting block fit together to limit the cover-in-cover.

6. The whole membrane multi-antibody incubation kit for Western Blot according to claim 1, characterized in that: The cover-in-cover is also provided with a handle.

Images