Conversion circuit and electricity conversion instrument

Abstract

The embodiment of the utility model provides a conversion circuit and an electricity conversion instrument, and belongs to the technical field of biological medicine equipment. The electricity conversion instrument comprises a battery, a conversion circuit, a signal modulation device and an electricity conversion cup. The conversion circuit comprises a charging module, a capacitor module and a discharging module. The battery is used for providing input voltage; the signal modulation device is electrically connected with the charging module and the discharging module and is used for providing an input pulse signal and a discharging control signal; wherein the discharge control signal is obtained by modulating an initial square wave signal by a signal modulation device based on a preset target waveform function; the capacitor module is charged based on an input pulse signal and an input voltage; and after the capacitor module is charged to a target capacitor voltage, the capacitor module performs discharging by using the discharging output sub-module to obtain a discharging signal, and the discharging output sub-module performs voltage conversion on the discharging signal based on the discharging control signal to obtain a target pulse signal. According to the embodiment of the invention, various types of pulses can be output, and the universality of the electricity conversion instrument is improved.

Description

Technical Field

[0001] The present application relates to the technical field of biomedical equipment, and in particular to a conversion circuit and an electrotransfer instrument. Background Art

[0002] Electroporators are specialized devices that can generate pulses and are used for electrotransfection. For example, they can apply electric pulses that instantly permeabilize cell membranes to help deliver drugs or genes into cells. An important factor affecting electrotransfection is the type of pulse. Currently, the pulse waveforms generated by the discharge of electroporators are limited, and they can generally only output fixed types of pulse signals. When electroporators perform electrotransfection on different cells, pulses of different waveforms are often required, and existing electroporators are difficult to meet this demand.

[0003] Therefore, how to increase the types of pulses of the electroporator and improve the diversity and universality of the electroporator has become a technical problem that needs to be solved urgently. Utility Model Content

[0004] The main purpose of the embodiments of the present application is to provide a conversion circuit and an electroporator, which are designed to output multiple types of pulses, increase the diversity of cell types that can be processed by the electroporator, and improve the universality of the electroporator.

[0005] To achieve the above-mentioned purpose, a first aspect of an embodiment of the present application provides an electrotransformer, comprising: a battery, a conversion circuit, a signal modulation device, and an electrotransformer cup; wherein the conversion circuit comprises a charging module, a capacitor module, and a discharge module;

[0006] The battery is electrically connected to the charging module, and the battery is used to provide an input voltage;

[0007] The signal modulation device is electrically connected to the charging module and the discharging module, and is used to provide an input pulse signal and a discharging control signal; wherein the discharging control signal is obtained by modulating the initial square wave signal by the signal modulation device based on a preset target waveform function;

[0008] The charging module is electrically connected to the capacitor module, and the capacitor module is charged based on the input pulse signal and the input voltage;

[0009] The discharge module is electrically connected to the capacitor module. After the capacitor module is charged to a target capacitor voltage, the capacitor module discharges to obtain a discharge signal. The discharge module performs voltage conversion on the discharge signal based on the discharge control signal to obtain a target pulse signal.

[0010] The electrotransfection cup is electrically connected to the discharge module, and the electrotransfection cup performs an electrotransfection operation based on the target pulse signal.

[0011] In some embodiments, the discharge module includes a discharge voltage stabilizing switch submodule and a discharge output submodule;

[0012] After the capacitor module is charged to the target capacitor voltage, the discharge voltage stabilizing switch submodule changes from an open state to a closed state;

[0013] When the discharge voltage stabilizing switch submodule is in a closed state, the capacitor module discharges using the discharge output submodule.

[0014] In some embodiments, the conversion circuit further includes a voltage feedback module, and the signal modulation device includes an analog-to-digital conversion submodule and a data processing submodule;

[0015] The analog-to-digital conversion submodule is electrically connected to the voltage feedback module and the data processing submodule, the voltage feedback module is electrically connected to the capacitor module, the voltage feedback module is used to collect the voltage signal of the capacitor module, and the analog-to-digital conversion submodule digitizes the voltage signal;

[0016] The data processing submodule obtains a feedback capacitor voltage according to the digitized voltage signal, and compares the feedback capacitor voltage with a target capacitor voltage;

[0017] If the feedback capacitor voltage is greater than the target capacitor voltage, the data processing submodule reduces the duty cycle of the input pulse signal so that the capacitor module is maintained at the target capacitor voltage.

[0018] To achieve the above-mentioned purpose, the second aspect of the embodiment of the present application proposes a conversion circuit, which is applied to the electric converter described in the first aspect, and the conversion circuit includes: a capacitor module, a charging module and a discharging module; wherein the discharging module includes a discharging voltage stabilizing switch submodule and a discharging output submodule, and the discharging voltage stabilizing switch submodule is electrically connected to the capacitor module and the discharging output submodule;

[0019] The charging module is electrically connected to the capacitor module, the capacitor module is charged based on the input pulse signal and the input voltage, and after charging to the target capacitor voltage, the discharge voltage stabilizing switch submodule is changed from an open state to a closed state;

[0020] The discharge module is electrically connected to the capacitor module. After the capacitor module is charged to a target capacitor voltage, the capacitor module uses the discharge output submodule to discharge to obtain a discharge signal. The discharge output submodule converts the discharge signal into a voltage based on the discharge control signal to obtain a target pulse signal; wherein the discharge control signal is obtained by modulating an initial square wave signal by a signal modulation device based on a preset target waveform function.

[0021] In some embodiments, the discharge voltage stabilizing switch submodule includes a first driving chip, a second driving chip, a first energy storage voltage stabilizing unit, a second energy storage voltage stabilizing unit, a first switch unit, a second switch unit, a third switch unit, and a fourth switch unit; the discharge output submodule includes a first discharge port and a second discharge port;

[0022] The first switch unit is electrically connected to the capacitor module, the first energy storage voltage stabilizing unit and the first driver chip, the second switch unit is electrically connected to the capacitor module, the second energy storage voltage stabilizing unit and the second driver chip, the third switch unit is electrically connected to the first energy storage voltage stabilizing unit and the first driver chip, and the fourth switch unit is electrically connected to the second energy storage voltage stabilizing unit and the second driver chip; the first energy storage voltage stabilizing unit is electrically connected to the second discharge port, and the second energy storage voltage stabilizing unit is electrically connected to the first discharge port;

[0023] When the discharge voltage stabilizing switch submodule is in the disconnected state, the first switch unit, the second switch unit, the third switch unit and the fourth switch unit are disconnected;

[0024] When the discharge voltage stabilizing switch submodule is in a closed state, the first switch unit, the third switch unit and the fourth switch unit are closed, or the second switch unit, the third switch unit and the fourth switch unit are closed.

[0025] In some embodiments, the discharge voltage stabilizing switch submodule controls the voltage polarity of the first discharge port and the voltage polarity of the second discharge port to control the type of the target pulse signal;

[0026] If the first switch unit, the third switch unit and the fourth switch unit are closed, and the second switch unit is open, the voltage polarity of the first discharge port is positive, the voltage polarity of the second discharge port is negative, and the voltage conversion is performed through the first energy storage voltage stabilization unit;

[0027] If the first switch unit is disconnected, and the second switch unit, the third switch unit and the fourth switch unit are closed, the voltage polarity of the first discharge port is negative, the voltage polarity of the second discharge port is positive, and voltage conversion is performed through the second energy storage voltage stabilization unit.

[0028] In some embodiments, the conversion circuit further includes a voltage feedback module;

[0029] The voltage feedback module is electrically connected to the capacitor module, and the voltage feedback module is used to detect the voltage of the capacitor module to obtain a feedback capacitor voltage;

[0030] The input pulse signal is provided by a signal modulation device; if the feedback capacitor voltage is greater than the target capacitor voltage, the signal modulation device reduces the duty cycle of the input pulse signal to maintain the capacitor module at the target capacitor voltage.

[0031] In some embodiments, the conversion circuit further includes a power leakage module;

[0032] The discharge module is electrically connected to the capacitor module, and is used for discharging electricity after the capacitor module has finished discharging, so as to release the remaining electricity of the capacitor module.

[0033] In some embodiments, the charging module includes a charging switch submodule;

[0034] Wherein, when the charging switch submodule is in a closed state, the capacitor module is charged to a target capacitor voltage based on the input pulse signal and the input voltage;

[0035] Before the power discharge module discharges power, the charging switch submodule is switched to the disconnected state, and the discharging voltage stabilizing switch submodule is switched to the disconnected state.

[0036] In some embodiments, the capacitor module includes a first electrolytic capacitor and a second electrolytic capacitor;

[0037] The positive electrode of the first electrolytic capacitor is electrically connected to the charging module, the discharging module and the positive electrode of the second electrolytic capacitor, and the negative electrode of the first electrolytic capacitor is grounded; the positive electrode of the second electrolytic capacitor is electrically connected to the charging module and the discharging module, and the negative electrode of the second electrolytic capacitor is grounded.

[0038] The conversion circuit and electrotransfection instrument proposed in the present application provide input pulse signals and discharge control signals through a signal modulation device; wherein the discharge control signal is obtained by modulating the initial square wave signal by the signal modulation device based on a preset target waveform function. The capacitor module is charged based on the input pulse signal and the input voltage. After the capacitor module is charged to the target capacitor voltage, the capacitor module is discharged to obtain a discharge signal. The discharge module performs voltage conversion on the discharge signal based on the discharge control signal, so that the output voltage meets the power requirement for driving the electrotransfection cup to perform the electrotransfection operation, and obtains the target pulse signal. The electrotransfection cup performs the electrotransfection operation based on the target pulse signal. For different pulse waveform requirements, the initial square wave signal is modulated by the signal modulation device to obtain the discharge control signal, so that the conversion circuit can output target pulse signals of different waveforms based on the discharge control signal, thereby outputting multiple types of pulses, improving the diversity of cell types that can be processed by the electrotransfection instrument, and improving the universality of the electrotransfection instrument. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 It is a module block diagram of the electroporator provided in the embodiment of the present application;

[0040] Figure 2 is a module block diagram of a conversion circuit provided in an embodiment of the present application;

[0041] Figure 3 This is provided by an embodiment of the present application Figure 2 Module block diagram of the discharge voltage stabilizing switch submodule 310 and the discharge output submodule 320;

[0042] Figure 4 is a module block diagram of a conversion circuit provided in another embodiment of the present application;

[0043] Figure 5 is a module block diagram of a conversion circuit provided in another embodiment of the present application;

[0044] Figure 6 It is a circuit schematic diagram of an application example provided in an embodiment of the present application. DETAILED DESCRIPTION

[0045] In order to make the purpose, technical solution and advantages of the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0046] It should be noted that, although the functional modules are divided in the device schematic diagram and the logical order is shown in the flowchart, in some cases, the steps shown or described may be performed in a different order than the module division in the device or the order in the flowchart. The terms "first", "second", etc. in the specification, claims and the above drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence.

[0047] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein are only for the purpose of describing the embodiments of this application and are not intended to limit this application.

[0048] First, some nouns involved in this application are analyzed:

[0049] Transfection: refers to the introduction of target genes into cells through biochemical or physical methods.

[0050] Electrotransfection: The use of electric pulses to apply a strong electric field can instantly increase the permeability of the cell membrane through the high-intensity electric field, thereby introducing macromolecules that cannot penetrate the cell membrane into the cell. The effect of electrotransfection depends on various parameters of the electric field, such as pulse type, pulse voltage, pulse duration, number of pulses, and other experimental conditions.

[0051] PWM (Pulse Width Modulation) is an analog circuit control method that controls the on and off of the switching devices in the inverter circuit so that the output end obtains a series of pulses with equal amplitude but inconsistent width, and uses these pulses to replace the sine wave or the required waveform.

[0052] The conversion circuit and the electrotransmitter provided in the embodiments of the present application are specifically described through the following embodiments. First, the electrotransmitter in the embodiments of the present application is described.

[0053] Figure 1 1 is a block diagram of a module of an electrotransformer provided in an embodiment of the present application. The electrotransformer includes: a battery, a conversion circuit, a signal modulation device, and an electrotransformer cup. The conversion circuit includes a capacitor module 100, a charging module 200, and a discharging module 300.

[0054] The battery is electrically connected to the charging module 200 , and the battery is used to provide an input voltage.

[0055] The signal modulation device is electrically connected to the charging module 200 and the discharging module 300, and is used to provide an input pulse signal and a discharging control signal, wherein the discharging control signal is obtained by modulating the initial square wave signal by the signal modulation device based on a preset target waveform function.

[0056] The charging module 200 is electrically connected to the capacitor module 100 , and the capacitor module 100 is charged based on the input pulse signal and the input voltage.

[0057] The discharge module 300 is electrically connected to the capacitor module 100. After the capacitor module 100 is charged to a target capacitor voltage, the capacitor module 100 discharges to obtain a discharge signal. The discharge module 300 converts the discharge signal into a voltage based on a discharge control signal to obtain a target pulse signal.

[0058] The electrotransfection cup is electrically connected to the discharge module 300 , and performs an electrotransfection operation based on the target pulse signal.

[0059] Specifically, the battery can be a lithium battery with a voltage of 12 V. The electrotransfection cup contains a liquid used in an electrotransfection experiment, such as a solution used in a cell transfection experiment.

[0060] Specifically, the signal modulation device may be a single chip microcomputer. In one embodiment, the signal modulation device may obtain a first modulation pulse signal modulated by a host computer based on a preset target waveform function, and obtain a discharge control signal after processing the first modulation pulse signal.

[0061] It should be noted that the input pulse signal and the discharge control signal may be PMW (pulse width modulation) signals.

[0062] It should be noted that the variation rule of the voltage waveform of the target pulse signal is associated with the variation rule of the target waveform function used by the signal modulation device to modulate the discharge control signal.

[0063] The cell electrotransfection experiment requires pulses of various waveforms. The beneficial effects of the embodiments of the present application include but are not limited to: providing an input pulse signal and a discharge control signal through a signal modulation device; wherein the discharge control signal is obtained by modulating the initial square wave signal by the signal modulation device based on a preset target waveform function. The capacitor module 100 is charged based on the input pulse signal and the input voltage. After the capacitor module 100 is charged to the target capacitor voltage, the capacitor module 100 is discharged to obtain a discharge signal. The discharge module 300 performs voltage conversion on the discharge signal based on the discharge control signal, so that the output signal power meets the power requirement of driving the electrotransfection cup for electrotransfection operation, and obtains the target pulse signal. The electrotransfection cup performs electrotransfection operation based on the target pulse signal. For different pulse waveform requirements, the initial square wave signal is modulated by the signal modulation device to obtain a discharge control signal, so that the conversion circuit outputs target pulse signals of different waveforms based on the discharge control signal, thereby outputting multiple types of pulses, improving the diversity of cell types that can be processed by the electrotransfection instrument, and improving the universality of the electrotransfection instrument.

[0064] In some embodiments, the power of the signal modulated by the signal modulation device does not reach the output power required by the electrotransfection instrument and cannot be directly used as a signal to drive the electrotransfection cup to perform electrotransfection. In this embodiment, the conversion circuit performs voltage conversion and amplifies the power of the signal so that the target pulse signal can drive the electrotransfection cup to perform electrotransfection.

[0065] In some embodiments, the discharge module 300 includes a discharge voltage stabilizing switch submodule 310 and a discharge output submodule 320;

[0066] After the capacitor module 100 is charged to the target capacitor voltage, the discharge voltage stabilizing switch submodule 310 changes from an open state to a closed state;

[0067] When the discharge voltage stabilizing switch submodule 310 is in a closed state, the capacitor module 100 discharges using the discharge output submodule 320 .

[0068] The advantage of this embodiment is that after the capacitor module 100 is charged to the target capacitor voltage, the discharge voltage stabilizing switch submodule 310 is switched from an open state to a closed state, and the capacitor module 100 is discharged using the discharge output submodule 320 .

[0069] In some embodiments, the conversion circuit further includes a voltage feedback module ( Figure 1 (not shown), the signal modulation device includes an analog-to-digital conversion submodule and a data processing submodule.

[0070] The analog-to-digital conversion submodule is electrically connected to the voltage feedback module and the data processing submodule. The voltage feedback module is used to collect the voltage signal of the capacitor module 100, and the analog-to-digital conversion submodule digitizes the voltage signal.

[0071] The data processing submodule obtains the feedback capacitor voltage according to the digitized voltage signal, and compares the feedback capacitor voltage with the target capacitor voltage;

[0072] If the feedback capacitor voltage is greater than the target capacitor voltage, the data processing submodule reduces the duty cycle of the input pulse signal to maintain the capacitor module 100 at the target capacitor voltage.

[0073] Specifically, the analog-to-digital conversion submodule may be an analog-to-digital converter (ADC), and the analog-to-digital conversion submodule is used to convert the feedback capacitor voltage from an analog signal to a digital signal. The data processing submodule is used to process the digital signal, such as data preprocessing, data calculation, data analysis, etc.

[0074] The advantage of this embodiment is that by detecting the feedback capacitor voltage, the voltage value of the capacitor in the capacitor module 100 is obtained, so as to avoid overvoltage of the capacitor module 100 to damage the circuit, thereby improving the stability of the circuit.

[0075] See also Figure 2 , in some embodiments, the conversion circuit further includes a charging module 200 and a discharging module 300, and the capacitor module 100 is electrically connected to the charging module 200 and the discharging module 300;

[0076] The charging module 200 is used to charge the capacitor module 100 based on the input pulse signal and the input voltage when the switch of the discharging module 300 is turned off;

[0077] The discharge module 300 is used to discharge the capacitor module 100 and perform voltage conversion based on the discharge control signal to obtain a target pulse signal when the difference between the feedback capacitor voltage and the target capacitor voltage is less than the voltage difference threshold.

[0078] It should be noted that the target capacitor voltage may be the rated voltage value of the capacitor in the capacitor module 100, which is not specifically limited in this embodiment. The voltage difference threshold is a preset feedback capacitor voltage and the target capacitor voltage.

[0079] The advantage of this embodiment is that, in response to different pulse waveform requirements, the initial square wave signal is modulated by the signal modulation device to obtain a discharge control signal, so that when the switch of the discharge module 300 is disconnected, the charging module 200 charges the capacitor module 100 based on the input pulse signal and the input voltage, and when the difference between the feedback capacitor voltage and the target capacitor voltage is less than the voltage difference threshold, the discharge module 300 discharges the capacitor module 100 based on the discharge control signal and performs voltage conversion to obtain a target pulse signal, thereby outputting multiple types of pulses, improving the diversity of cell types that can be processed by the electroporator, and improving the universality of the electroporator.

[0080] Figure 2 1 is a block diagram of a conversion circuit provided in an embodiment of the present application. The conversion circuit is applied to the above-mentioned electric converter, and the conversion circuit includes: a capacitor module 100, a charging module 200 and a discharging module 300. Among them, the discharging module 300 includes a discharging voltage stabilizing switch submodule 310 and a discharging output submodule 320.

[0081] The capacitor module 100 is electrically connected to the charging module 200 and the discharging module 300 , and the discharging voltage-stabilizing switch submodule 310 is electrically connected to the capacitor module 100 and the discharging output submodule 320 .

[0082] The charging module 200 is used to obtain the input pulse signal and the input voltage, so that when the discharge voltage stabilizing switch submodule 310 is in the open state, the capacitor module 100 is charged to the target capacitor voltage based on the input pulse signal and the input voltage, so that the discharge voltage stabilizing switch submodule 310 is turned into a closed state.

[0083] The discharge module 300 is used to discharge the capacitor module 100 when the discharge voltage stabilizing switch submodule 310 is in a closed state. The discharge voltage stabilizing switch submodule 310 performs voltage conversion on the voltage generated during the discharge process of the capacitor module 100 based on the discharge control signal, and the discharge output submodule 320 outputs the converted voltage to obtain a target pulse signal. The discharge control signal is obtained by modulating the initial square wave signal by the signal modulation device based on a preset target waveform function.

[0084] Specifically, the input voltage may be a 12V DC voltage provided by a battery.

[0085] It should be noted that the frequency of the input pulse signal is relatively high, and the input pulse signal may be a PMW (pulse width modulation) signal. For example, a common electric converter uses a 100KHZ (kilohertz) PWM signal for charging, but the embodiment of the present application uses a 2MHz (megahertz) input pulse signal to charge the capacitor module 100. The embodiment of the present application boosts the input voltage by an input pulse signal with a relatively high frequency, and the capacitor module 100 is quickly charged to the target capacitor voltage based on the boosted voltage, thereby increasing the charging speed.

[0086] It should be noted that the capacitance of the capacitor module 100 is relatively small. For example, in a common electric converter, the capacitance is 1011, but the capacitance value of the capacitor module 100 in the embodiment of the present application is 111, that is, the embodiment of the present application selects one-tenth of its capacitance. The embodiment of the present application shortens the charging time required for the electric converter by reducing the capacitance of the capacitor module 100. A common electric converter takes about 20 seconds to charge the capacitor, and the capacitor discharge time after charging is generally 1ms to 10ms. The charging time of this embodiment can be shortened to about 1s.

[0087] It should be noted that, in this embodiment, the switch in the discharge voltage stabilizing switch submodule 310 is switched between a closed state and an open state, so that the capacitor module 100 can quickly switch between a charging state and a discharging state, thereby continuously outputting a pulse signal, so that the electroconverter can continuously generate electrical pulses.

[0088] The beneficial effects of the embodiments of the present application include but are not limited to obtaining the input pulse signal and the input voltage through the charging module 200, so that when the discharge voltage stabilizing switch submodule 310 is in the disconnected state, the capacitor module 100 is charged to the target capacitor voltage based on the input pulse signal and the input voltage, and then the discharge voltage stabilizing switch submodule 310 is turned to the closed state to discharge the capacitor module 100. The discharge voltage stabilizing switch submodule 310 performs voltage conversion on the voltage generated during the discharge process of the capacitor module 100 based on the discharge control signal, so that the output voltage meets the power requirement of driving the electrotransfection cup for electrotransfection operation. Among them, the discharge control signal is obtained by modulating the initial square wave signal by the signal modulation device based on the preset target waveform function. The discharge control signal is obtained by modulating the initial square wave signal by the signal modulation device, so that the conversion circuit outputs target pulse signals of different waveforms based on the discharge control signal, thereby outputting multiple types of pulses. The discharge output submodule 320 outputs the converted voltage to obtain the target pulse signal. The embodiments of the present application can improve the diversity of cell types that can be processed by the electrotransfection instrument and improve the universality of the electrotransfection instrument.

[0089] See also Figure 2 and Figure 3In some embodiments, the discharge voltage stabilization switch submodule 310 includes a first driver chip, a second driver chip, a first energy storage voltage stabilization unit, a second energy storage voltage stabilization unit, a first switch unit, a second switch unit, a third switch unit, and a fourth switch unit. The discharge output submodule 320 includes a first discharge port and a second discharge port.

[0090] The first switch unit is electrically connected to the capacitor module 100, the first energy storage voltage stabilizing unit and the first driver chip, the second switch unit is electrically connected to the capacitor module 100, the second energy storage voltage stabilizing unit and the second driver chip, the third switch unit is electrically connected to the first energy storage voltage stabilizing unit and the first driver chip, and the fourth switch unit is electrically connected to the second energy storage voltage stabilizing unit and the second driver chip; the first energy storage voltage stabilizing unit is electrically connected to the second discharge port, and the second energy storage voltage stabilizing unit is electrically connected to the first discharge port.

[0091] When the discharge voltage stabilizing switch submodule 310 is in the off state, the first switch unit, the second switch unit, the third switch unit and the fourth switch unit are disconnected.

[0092] When the discharge voltage stabilizing switch submodule 310 is in a closed state, the first switch unit, the third switch unit and the fourth switch unit are closed, or the second switch unit, the third switch unit and the fourth switch unit are closed.

[0093] Specifically, the models of the first driver chip and the second driver chip may be IR2181S.

[0094] It should be noted that the discharge control signal includes a first control signal, a second control signal, a third control signal and a fourth control signal. The first driver chip controls the first switch unit based on the first control signal, and controls the third switch unit based on the third control signal. The second driver chip controls the second switch unit based on the second control signal, and controls the fourth switch unit based on the fourth control signal.

[0095] It should be noted that the first discharge port is electrically connected to the first electrode of the electro-rotating cup, and the second discharge port is electrically connected to the second electrode of the electro-rotating cup. Specifically, the first electrode may be the positive electrode of the electro-rotating cup, and the second electrode may be the negative electrode of the electro-rotating cup.

[0096] The advantage of this embodiment is that by controlling the first switch unit, the second switch unit, the third switch unit and the fourth switch unit to be disconnected, so that the discharge voltage stabilization switch submodule 310 is in an off state, the capacitor module 1001 is quickly charged through the charging module 200 so that the voltage of the capacitor module 100 reaches the target capacitor voltage. The discharge voltage stabilization switch submodule 310 is switched to a closed state to discharge the capacitor module 100, and the target pulse signal is output from the discharge output submodule 320.

[0097] See also Figure 2and Figure 3 In some embodiments, the discharge voltage stabilizing switch submodule 310 controls the voltage polarity of the first discharge port and the voltage polarity of the second discharge port to control the type of the target pulse signal.

[0098] If the first switch unit, the third switch unit and the fourth switch unit are closed, and the second switch unit is open, the voltage polarity of the first discharge port is positive, the voltage polarity of the second discharge port is negative, and voltage conversion is performed through the first energy storage voltage stabilization unit.

[0099] If the first switch unit is disconnected, and the second switch unit, the third switch unit and the fourth switch unit are closed, the voltage polarity of the first discharge port is negative, the voltage polarity of the second discharge port is positive, and voltage conversion is performed through the second energy storage voltage stabilization unit.

[0100] Specifically, after the capacitor module 100 is charged to the target capacitor voltage, if the first switch unit, the third switch unit and the fourth switch unit are closed, and the second switch unit is disconnected, the first energy storage voltage stabilizing unit, the first switch unit, the third switch unit and the fourth switch unit form a BUCK circuit with the capacitor module 100 as a power source. The circuit controls the duty cycle of the first switch unit based on the discharge control signal, thereby controlling the voltage of the target pulse signal output from the discharge output terminal. In this case, the voltage polarity of the first discharge port is positive, and the voltage polarity of the second discharge port is negative.

[0101] Specifically, after the capacitor module 100 is charged to the target capacitor voltage, if the second switch unit, the third switch unit and the fourth switch unit are closed and the first switch unit is disconnected, the second energy storage voltage stabilizing unit, the second switch unit, the third switch unit and the fourth switch unit form a BUCK circuit with the capacitor module 100 as a power source. The circuit controls the duty cycle of the second switch unit based on the discharge control signal, thereby controlling the voltage of the target pulse signal output from the discharge output terminal. In this case, the voltage polarity of the first discharge port is negative, and the voltage polarity of the second discharge port is positive.

[0102] It should be noted that the BUCK circuit (step-down conversion circuit) is a DC-DC conversion circuit based on the inductive energy storage principle.

[0103] The advantage of this embodiment is that, during the discharge process, when the first switch unit, the third switch unit, and the fourth switch unit are in a closed state, and the second switch unit is in an open state, a current polarity state in which the polarity of the first discharge port is positive and the polarity of the second discharge port is negative is obtained. When the first switch unit is in an open state, and the second switch unit, the third switch unit, and the fourth switch unit are in a closed state, another current polarity state in which the polarity of the first discharge port is negative and the polarity of the second discharge port is positive is obtained. By controlling the first switch unit, the second switch unit, the third switch unit, and the fourth switch unit to adjust the period of switching between these two current polarity states, the waveform of the target pulse signal output by the discharge output submodule 320 can be controlled.

[0104] See also Figure 4 In some embodiments, the conversion circuit further includes a voltage feedback module 400. The voltage feedback module 400 is electrically connected to the capacitor module 100, and the voltage feedback module 400 is used to detect the voltage of the capacitor module 100 to obtain a feedback capacitor voltage;

[0105] The input pulse signal is provided by a signal modulation device. If the feedback capacitor voltage is greater than the target capacitor voltage, the signal modulation device reduces the duty cycle of the input pulse signal to maintain the capacitor module 100 at the target capacitor voltage.

[0106] The advantage of this embodiment is that the voltage of the capacitor module 100 is detected by the voltage feedback module 400 to obtain the feedback capacitor voltage. If the feedback capacitor voltage is greater than the target capacitor voltage, the signal generating device reduces the duty cycle of the input pulse signal according to the feedback capacitor voltage, so that the capacitor module 100 is maintained at the target capacitor voltage, thereby improving the stability of the circuit.

[0107] See also Figure 5 In some embodiments, the conversion circuit further includes a discharge module 500. The discharge module 500 is electrically connected to the capacitor module 100, and is used to discharge electricity after the capacitor module 100 is discharged to release the remaining electricity of the capacitor module 100.

[0108] The advantage of this embodiment is that, through the discharge module 500, the residual electricity of the capacitor module 100 is released after the discharge process is completed, thereby improving the safety of the electrocirculator.

[0109] In some embodiments, the charging module 200 includes a charging switch submodule. When the charging switch submodule is in a closed state, the capacitor module 100 is charged to a target capacitor voltage based on the input pulse signal and the input voltage. Before the discharge module 500 discharges electricity, the charging switch submodule is turned off, and the discharge voltage stabilizing switch submodule 310 is turned off.

[0110] The advantage of this embodiment is that the input pulse signal and the input voltage are obtained through the charging module 200, and the input voltage is boosted by the input pulse signal with a higher frequency, so that when the charging switch submodule is in a closed state and the discharge voltage stabilizing switch submodule 310 is in an open state, the capacitor module 100 with a lower capacitance is quickly charged based on the boosted voltage so that the voltage of the capacitor module 100 reaches the target capacitor voltage, thereby improving the charging speed of the electric converter and shortening the charging time of the electric converter.

[0111] In some embodiments, the capacitor module 100 includes a first electrolytic capacitor and a second electrolytic capacitor. The positive electrode of the first electrolytic capacitor is electrically connected to the charging module 200, the discharging module 300, and the positive electrode of the second electrolytic capacitor, and the negative electrode of the first electrolytic capacitor is grounded; the positive electrode of the second electrolytic capacitor is electrically connected to the charging module 200 and the discharging module 300, and the negative electrode of the second electrolytic capacitor is grounded.

[0112] The advantage of this embodiment is that the capacitor module 100 includes a first electrolytic capacitor and a second electrolytic capacitor, and the overall capacitance of the capacitor module 100 is relatively small. By reducing the capacitance of the capacitor module 100, the charging time required for the electroconverter is shortened, and the electroconverter can be fully charged quickly and quickly switched between the charging state and the discharging state, so that the electroconverter can continuously generate electrical pulses.

[0113] See also Figure 5 and Figure 6 In an application example, the circuit and specific circuit connection relationship of the charging and discharging circuit are as follows: Figure 6 shown.

[0114] The capacitor module 100 includes a first electrolytic capacitor C6B and a second electrolytic capacitor C7B.

[0115] The charging module 200 includes a power input terminal CELL, a signal input terminal PWMP, a first MOS tube Q1, a first inductor L1, a first diode D1 and a first resistor R1. The power input terminal CELL is electrically connected to a battery, which may be a lithium battery with a voltage of 12V.

[0116] The discharge module 300 includes a first control terminal CTRL1, a second control terminal CTRL2, a third control terminal CTRL3, a fourth control terminal CTRL4, a voltage-stabilized power supply terminal VCC, a first driver chip U1, a second driver chip U2, a third MOS transistor Q3, a fourth MOS transistor Q4, a fifth MOS transistor Q5, a sixth MOS transistor Q6, a second capacitor C2, a third capacitor C3, a fourth capacitor C4, a fifth capacitor C5, a second diode D2, a third diode D3, a fourth diode D4, a fifth diode D5, a sixth diode D6, a seventh diode D7, a sixth resistor R6, a seventh resistor R7, an eighth resistor R8, a ninth resistor R9, a tenth resistor R10, an eleventh resistor R11, a twelfth resistor R12, a thirteenth resistor R13, a fourteenth resistor R14, a fifteenth resistor R15, a sixteenth resistor R16, a seventeenth resistor R17 and an eighteenth resistor R18.

[0117] The voltage feedback module 400 includes an analog-to-digital conversion terminal ADCP, a first capacitor C1, a fourth resistor R4 and a fifth resistor R5.

[0118] The discharge module 500 includes a discharge terminal DISCHARGE, a second MOS transistor Q2, a second resistor R2 and a third resistor R3.

[0119] It should be noted that the voltage-stabilized power supply terminal VCC is used to supply power to the first driver chip U1 and the second driver chip U2, and can provide an AC voltage of 12V.

[0120] It should be noted that in the discharge module 300, the discharge output submodule 320 is specifically the discharge output terminal CN1, the first discharge port is the interface No. 1 of the discharge output terminal CN1, and the second discharge port is the interface No. 2 of the discharge output terminal CN1. The discharge voltage stabilizing switch submodule 310 includes circuit elements in the discharge module 300 except the discharge output terminal CN1.

[0121] It should be noted that the first switch unit refers to the third MOS tube Q3, the second switch unit refers to the fourth MOS tube Q4, the third switch unit refers to the fifth MOS tube Q5, and the fourth switch unit refers to the sixth MOS tube Q6.

[0122] It should be noted that the charging switch submodule refers to the first MOS tube Q1.

[0123] The embodiments described in the embodiments of the present application are intended to more clearly illustrate the technical solutions of the embodiments of the present application and do not constitute a limitation on the technical solutions provided in the embodiments of the present application. Those skilled in the art will appreciate that with the evolution of technology and the emergence of new application scenarios, the technical solutions provided in the embodiments of the present application are also applicable to similar technical problems.

[0124] Those skilled in the art will appreciate that the technical solutions shown in the figures do not constitute a limitation on the embodiments of the present application, and may include more or fewer steps than shown in the figures, or a combination of certain steps, or different steps.

[0125] The device embodiments described above are merely illustrative, and the units described as separate components may or may not be physically separated, that is, they may be located in one place or distributed on multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0126] Those skilled in the art will appreciate that all or some of the steps in the methods disclosed above, and the functional modules / units in the systems and devices may be implemented as software, firmware, hardware, or a suitable combination thereof.

[0127] The terms "first", "second", "third", "fourth", etc. (if any) in the specification of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchangeable where appropriate, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "including" and "having" and any of their variations are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device comprising a series of steps or units is not necessarily limited to those steps or units clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0128] It should be understood that in the present application, "at least one (item)" means one or more, and "plurality" means two or more. "And / or" is used to describe the association relationship of associated objects, indicating that three relationships may exist. For example, "A and / or B" can mean: only A exists, only B exists, and A and B exist at the same time, where A and B can be singular or plural. The character " / " generally indicates that the objects associated before and after are in an "or" relationship. "At least one of the following" or similar expressions refers to any combination of these items, including any combination of single or plural items. For example, at least one of a, b or c can mean: a, b, c, "a and b", "a and c", "b and c", or "a and b and c", where a, b, c can be single or multiple.

[0129] In the several embodiments provided in the present application, it should be understood that the disclosed devices and methods can be implemented in other ways. For example, the device embodiments described above are only schematic. For example, the division of the above units is only a logical function division. There may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. The mutual coupling or direct coupling or communication connection shown or discussed can be through some interfaces, indirect coupling or communication connection of devices or units, which can be electrical, mechanical or other forms.

[0130] The units described above as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0131] The preferred embodiments of the present invention are described above with reference to the accompanying drawings, but the scope of the rights of the present invention is not limited thereto. Any modification, equivalent substitution and improvement made by a person skilled in the art without departing from the scope and essence of the present invention should be within the scope of the rights of the present invention.

Claims

1. An electroporator, characterized in that: include: A battery, a conversion circuit, a signal modulation device, and an electric rotating cup; wherein the conversion circuit includes a charging module, a capacitor module, and a discharge module; The battery is electrically connected to the charging module, and the battery is used to provide an input voltage; The signal modulation device is electrically connected to the charging module and the discharging module, and is used to provide an input pulse signal and a discharging control signal; wherein the discharging control signal is obtained by modulating the initial square wave signal by the signal modulation device based on a preset target waveform function; The charging module is electrically connected to the capacitor module, and the capacitor module is charged based on the input pulse signal and the input voltage; The discharge module is electrically connected to the capacitor module. After the capacitor module is charged to a target capacitor voltage, the capacitor module discharges to obtain a discharge signal. The discharge module performs voltage conversion on the discharge signal based on the discharge control signal to obtain a target pulse signal. The electrotransfection cup is electrically connected to the discharge module, and the electrotransfection cup performs an electrotransfection operation based on the target pulse signal.

2. The electroporator according to claim 1, characterized in that: The discharge module includes a discharge voltage stabilizing switch submodule and a discharge output submodule; After the capacitor module is charged to the target capacitor voltage, the discharge voltage stabilizing switch submodule changes from an open state to a closed state; When the discharge voltage stabilizing switch submodule is in a closed state, the capacitor module discharges using the discharge output submodule.

3. The electroporator according to claim 1, characterized in that: The conversion circuit further includes a voltage feedback module, and the signal modulation device includes an analog-to-digital conversion submodule and a data processing submodule; The analog-to-digital conversion submodule is electrically connected to the voltage feedback module and the data processing submodule, the voltage feedback module is electrically connected to the capacitor module, the voltage feedback module is used to collect the voltage signal of the capacitor module, and the analog-to-digital conversion submodule digitizes the voltage signal; The data processing submodule obtains a feedback capacitor voltage according to the digitized voltage signal, and compares the feedback capacitor voltage with a target capacitor voltage; If the feedback capacitor voltage is greater than the target capacitor voltage, the data processing submodule reduces the duty cycle of the input pulse signal so that the capacitor module is maintained at the target capacitor voltage.

4. A conversion circuit, characterized in that: Applied to the electroconverter according to any one of claims 1 to 3, the conversion circuit comprises: a capacitor module, a charging module and a discharging module; wherein the discharging module comprises a discharging voltage stabilizing switch submodule and a discharging output submodule, and the discharging voltage stabilizing switch submodule is electrically connected to the capacitor module and the discharging output submodule; The charging module is electrically connected to the capacitor module, the capacitor module is charged based on the input pulse signal and the input voltage, and after charging to the target capacitor voltage, the discharge voltage stabilizing switch submodule is changed from an open state to a closed state; The discharge module is electrically connected to the capacitor module. After the capacitor module is charged to a target capacitor voltage, the capacitor module uses the discharge output submodule to discharge to obtain a discharge signal. The discharge output submodule converts the discharge signal into a voltage based on the discharge control signal to obtain a target pulse signal; wherein the discharge control signal is obtained by modulating an initial square wave signal by a signal modulation device based on a preset target waveform function.

5. The conversion circuit according to claim 4, characterized in that: The discharge voltage stabilizing switch submodule includes a first driving chip, a second driving chip, a first energy storage voltage stabilizing unit, a second energy storage voltage stabilizing unit, a first switch unit, a second switch unit, a third switch unit and a fourth switch unit; the discharge output submodule includes a first discharge port and a second discharge port; The first switch unit is electrically connected to the capacitor module, the first energy storage voltage stabilizing unit and the first driver chip, the second switch unit is electrically connected to the capacitor module, the second energy storage voltage stabilizing unit and the second driver chip, the third switch unit is electrically connected to the first energy storage voltage stabilizing unit and the first driver chip, and the fourth switch unit is electrically connected to the second energy storage voltage stabilizing unit and the second driver chip; the first energy storage voltage stabilizing unit is electrically connected to the second discharge port, and the second energy storage voltage stabilizing unit is electrically connected to the first discharge port; When the discharge voltage stabilizing switch submodule is in the disconnected state, the first switch unit, the second switch unit, the third switch unit and the fourth switch unit are disconnected; When the discharge voltage stabilizing switch submodule is in a closed state, the first switch unit, the third switch unit and the fourth switch unit are closed, or the second switch unit, the third switch unit and the fourth switch unit are closed.

6. The conversion circuit according to claim 5, characterized in that: The discharge voltage stabilizing switch submodule controls the voltage polarity of the first discharge port and the voltage polarity of the second discharge port to control the type of the target pulse signal; If the first switch unit, the third switch unit and the fourth switch unit are closed, and the second switch unit is open, the voltage polarity of the first discharge port is positive, the voltage polarity of the second discharge port is negative, and the voltage conversion is performed through the first energy storage voltage stabilization unit; If the first switch unit is disconnected, and the second switch unit, the third switch unit and the fourth switch unit are closed, the voltage polarity of the first discharge port is negative, the voltage polarity of the second discharge port is positive, and voltage conversion is performed through the second energy storage voltage stabilization unit.

7. The conversion circuit according to claim 4, characterized in that: The conversion circuit also includes a voltage feedback module; The voltage feedback module is electrically connected to the capacitor module, and the voltage feedback module is used to detect the voltage of the capacitor module to obtain a feedback capacitor voltage; The input pulse signal is provided by a signal modulation device; if the feedback capacitor voltage is greater than the target capacitor voltage, the signal modulation device reduces the duty cycle of the input pulse signal to maintain the capacitor module at the target capacitor voltage.

8. The conversion circuit according to claim 4, characterized in that: The conversion circuit also includes a power leakage module; The discharge module is electrically connected to the capacitor module, and is used for discharging electricity after the capacitor module has finished discharging, so as to release the remaining electricity of the capacitor module.

9. The conversion circuit according to claim 8, characterized in that: The charging module includes a charging switch submodule; Wherein, when the charging switch submodule is in a closed state, the capacitor module is charged to a target capacitor voltage based on the input pulse signal and the input voltage; Before the power discharge module discharges power, the charging switch submodule is switched to the disconnected state, and the discharging voltage stabilizing switch submodule is switched to the disconnected state.

10. The conversion circuit according to claim 4, characterized in that: The capacitor module includes a first electrolytic capacitor and a second electrolytic capacitor; The positive electrode of the first electrolytic capacitor is electrically connected to the charging module, the discharging module and the positive electrode of the second electrolytic capacitor, and the negative electrode of the first electrolytic capacitor is grounded; the positive electrode of the second electrolytic capacitor is electrically connected to the charging module and the discharging module, and the negative electrode of the second electrolytic capacitor is grounded.

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