Centrifugal tube for detecting alkaloid in health food

Abstract

The utility model belongs to the technical field of chemical analysis articles, and particularly relates to a centrifugal tube for detecting alkaloid in health food, which comprises a sealing cover, a filter short tube, a liquid separation tube and an adapting tube, and the upper end and the lower end of the filter short tube are respectively connected with the sealing cover and the upper end of the liquid separation tube through threaded connection structures. The bottom of the liquid distributing pipe is a conical bottom, a liquid discharging pipe opening is formed in the center of the conical bottom, a supporting platform on which a filter membrane can be laid is arranged on the filter short pipe, an open hole is formed in the supporting platform, and a plug connecting rod for blocking the liquid discharging pipe opening is rotationally arranged on the supporting platform through a bearing; according to the centrifugal tube, filtration, centrifugation and liquid separation operation can be completed in the centrifugal tube, operation is simple, disturbance to liquid during liquid separation is effectively reduced, target liquid directly flows into the liquid separation device, material loss or introduction of other pollutants is avoided, and the centrifugal tube is suitable for large-scale production. The liquid separation efficiency is high and the effect is good.

Description

Technical Field

[0001] The utility model belongs to the technical field of chemical analysis products, and in particular relates to a centrifuge tube for detecting alkaloids in health food. Background Art

[0002] Plant-derived health foods contain a variety of alkaloids, such as harmaline, colchicine, vinblastine, vinblastine, papaverine, atropine, scopolamine, etc. The detection process of alkaloids in health foods generally requires sample extraction, separation, extraction purification, and liquid chromatography-mass spectrometry / mass spectrometry detection. Since the samples often contain matrix components such as protein and fat, the extract solution produces emulsification, and centrifuge tubes are often required for centrifugation. The centrifugation operation can separate the experimental extraction solution from the sample matrix. After the extract is filtered, centrifuged, and layered, it is often necessary to transfer the solution in the centrifuge tube to a separatory funnel for liquid-liquid separation. The operation steps are many, the operation is difficult, and it is easy to lose materials. It is also easy to introduce other pollutants. In addition, the liquid is often disturbed during the separation operation, causing the layered liquid to re-mix, resulting in poor separation effect, time-consuming and labor-intensive, affecting the experimental efficiency and results. Summary of the invention

[0003] In view of the above problems, the purpose of the utility model is to provide a centrifuge tube for centrifugal precipitation and liquid-liquid separation in the process of alkaloid detection in health food. Filtration, centrifugation and liquid separation operations can be completed in the centrifuge tube. The operation is simple and the disturbance of the liquid during liquid separation can be effectively reduced. The target liquid flows directly into the liquid separation device to avoid material loss or introduction of other pollutants. The liquid separation efficiency is high and the effect is good.

[0004] The purpose of the utility model is achieved through the following technical solutions: a centrifuge tube for detecting alkaloids in health food, comprising a sealing cover, a filtering short tube, a liquid dispensing tube, and a receiving tube, the upper end of the filtering short tube is connected to the sealing cover by a threaded connection structure, and the lower end is connected to the upper end of the liquid dispensing tube by a threaded connection structure, the bottom of the liquid dispensing tube is a conical bottom and a drainage pipe opening is arranged at the center of the conical bottom, a supporting platform on which a filter membrane can be laid is arranged on the filtering short tube, the supporting platform and the side wall of the filtering short tube form a groove to prevent liquid from overflowing, an opening for filtering is arranged on the supporting platform, a plug connecting rod for blocking the drainage pipe opening is rotatably arranged at the center of the supporting platform facing the drainage pipe opening through a bearing, a plug matching the drainage pipe opening is arranged at the lower end of the plug connecting rod, the upper end of the receiving tube is connected and coaxially sleeved on the outer wall of the liquid dispensing tube by a detachable sealing connection structure, and a cavity for receiving liquid is formed in the receiving tube between the lower end of the liquid dispensing tube and the bottom of the receiving tube.

[0005] Furthermore, the support platform is formed by multiple support rods integrally connected to the inner wall of the filter short tube, and the multiple support rods are arranged radially along the filter short tube. The center of the filter short tube is integrally connected with a vertical short tube through the support rods, and the upper end of the vertical short tube is flush with the upper end of the filter short tube. The plug connecting rod is rotatably installed on the vertical short tube through a bearing, and an opening for filtering is formed between two adjacent support rods.

[0006] Furthermore, the detachable sealing connection structure is a slot connection structure, which can play a role in sealing connection and positioning.

[0007] Furthermore, the slot connection structure includes a sealing ring 1 integrally connected to the outer wall of the dispensing tube, the bottom of the sealing ring 1 is integrally connected to a latch tongue, the top outer wall of the receiving tube is integrally connected to a sealing ring 2, and a slot matching the latch tongue is provided on the sealing ring 2 corresponding to the position of the latch tongue, and the dispensing tube and the receiving tube can be quickly plugged in to achieve a sealed sleeve connection.

[0008] Furthermore, the plug is an ellipsoidal or nearly conical rubber plug that matches the conical bottom shape of the liquid dispensing tube. The side wall of the rubber plug is designed to be arc-shaped, and the discharge pipe outlet is opened at the lower end of the conical bottom of the liquid dispensing tube near the top angle. The arc-shaped design of the side wall of the rubber plug can reduce the contact area between the side wall of the plug and the conical bottom during sealing, thereby reducing the pressure to achieve sealing and the pulling force required to lift the plug during discharge.

[0009] Furthermore, the conical bottom of the liquid dispensing tube is a pointed cone with a taper of not less than 75 degrees. When draining liquid from the bottom, due to the small volume of the bottom of the pointed cone, less liquid can be accommodated. When the plug is pulled up to drain liquid, the disturbance of the liquid in the liquid dispensing tube can be reduced, and it is convenient to observe the progress of liquid dispensing and seal it in time when liquid dispensing is completed.

[0010] Furthermore, a sealing structure for preventing leakage is provided between the discharge pipe outlet and the plug, and the sealing structure comprises an annular groove for installing a sealing ring provided on the bottom end of the conical bottom or the discharge pipe outlet, and an elastic sealing ring is installed in the annular groove.

[0011] Furthermore, a conical tube is integrally connected to the drainage pipe mouth, the small end of the conical tube extends downward from the drainage pipe mouth, the large end of the conical tube is higher than the lower end of the conical bottom, and the side wall of the conical tube is provided with a plurality of drainage holes, the bottoms of the plurality of drainage holes are flush with the bottom of the conical bottom, the side wall of the plug is adapted to the inner wall of the conical tube, and since the conical tube is much smaller than the conical bottom of the liquid dispensing tube, the inner wall of the conical tube cooperates with the side wall of the plug to achieve sealing of the liquid, which causes little disturbance to the liquid in the liquid dispensing tube, and the design of the drainage hole prevents the liquid from flowing too fast during drainage to form a vortex at the drainage port, thereby better avoiding liquid disturbance.

[0012] Furthermore, a butterfly-shaped knob is provided on the filtering short tube for easy turning.

[0013] Furthermore, the liquid dispensing tube is provided with a limiting structure to prevent the plug and the liquid discharge pipe opening from being blocked too tightly.

[0014] Furthermore, the inner ring of the bearing is interference fit with the plug connecting rod.

[0015] When the utility model is used, the upper and lower ends of the filter short tube are connected to the sealing cover and the upper end of the liquid separation tube through a threaded connection structure respectively. When in use, the sealing cover is opened, and the filter short tube is screwed until the plug at the lower end of the plug connecting rod is downward to block the discharge pipe opening to prevent liquid leakage. The sample is injected into the liquid separation tube through the opening arranged on the supporting platform, and the sample is filtered through the filter membrane on the supporting platform. The upper end of the receiving tube is connected to the outer wall of the liquid separation tube through a detachable sealing connection structure and is coaxially sleeved. When the sample needs to be separated after centrifugation and stratification, the reverse When the filter short tube is twisted, the plug connecting rod moves upward with the twisting of the filter short tube, and the plug at the lower end of the plug connecting rod is separated from the discharge pipe port under the action of tension, and the liquid is directly discharged into the receiving pipe from the discharge pipe port. Since the plug connecting rod is rotatably arranged at the center of the support platform through a bearing, when the filter short tube is twisted, the plug connecting rod will not rotate with the rotation of the filter short tube, thereby reducing the disturbance to the liquid. At the same time, since the lifting and lowering of the plug connecting rod is controlled by twisting the filter short tube, it is convenient to control the lifting and lowering speed and amplitude of the plug, thereby avoiding severe disturbance to the liquid during plugging or draining.

[0016] Beneficial effects: The utility model can realize filtering, centrifugation and liquid separation operations in a centrifuge tube, the operation is simple, and the disturbance of the liquid during liquid separation is effectively reduced. The target liquid flows directly into the liquid separation device to avoid material loss or introduction of other pollutants. The liquid separation efficiency is high and the effect is good. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The specific structure of this application is given by the following drawings and embodiments:

[0018] Figure 1 : A schematic diagram of the structure of Embodiment 1 of the present utility model;

[0019] Figure 2 : Schematic diagram of the installation structure of the filter short tube in Example 1 of the utility model;

[0020] Figure 3 : Schematic diagram of the structure of the upper support platform of the filter short tube of Example 1 of the utility model;

[0021] Figure 4 : Schematic diagram of the filter membrane structure of Example 1 of the utility model;

[0022] Figure 5: Schematic diagram of the connection structure of the slots on the liquid dispensing tube and the receiving tube in Example 1 of the utility model;

[0023] Figure 6 : A schematic structural diagram of the discharge nozzle and the plug of the liquid dispensing tube in Example 2 of the present utility model.

[0024] In the figure: 01. filter membrane, 1. sealing cover, 2. filter short tube, 3. dispensing tube, 4. receiving tube, 5. bearing, 6. plug connecting rod, 7. detachable sealing connection structure, 11. ventilation pipe opening, 21. supporting platform, 22. groove, 23. opening, 24 supporting rod, 25. vertical short tube, 26. butterfly knob, 31. conical bottom, 32. drainage pipe opening, 33. sealing structure, 34. limiting structure, 35. cone tube, 36. drainage hole, 61. plug, 71. slot connection structure, 72. sealing ring 1, 73. tongue, 74. sealing ring 2, 75. slot. DETAILED DESCRIPTION

[0025] The following is a specific implementation of the utility model to further explain the invention content in detail. This does not limit the patent scope of the utility model. Any equivalent structure or equivalent process transformation made by using the contents of the utility model specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the utility model.

[0026] In the present invention, for the convenience of description, the relative position relationship of each component is described according to the layout of the drawings in the specification, such as: the position relationship of up, down, left, right, etc. is determined according to the layout direction in the drawings in the specification.

[0027] Example 1, reference Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5A centrifuge tube for detecting alkaloids in health food, comprising a sealing cover 1, a filtering short tube 2, a liquid dispensing tube 3, and a receiving tube 4. The upper end of the filtering short tube 2 is connected to the sealing cover 1 through a threaded connection structure, and the lower end of the filtering short tube 2 is connected to the upper end of the liquid dispensing tube 3 through a threaded connection structure. The bottom of the liquid dispensing tube 3 is a conical bottom 31 and a drainage pipe port 32 is provided at the center of the conical bottom 31. A supporting platform 21 on which a filter membrane 01 can be laid is provided on the filtering short tube 2. The supporting platform 21 and the side wall of the filtering short tube 2 form a groove to prevent liquid from overflowing. 22, an opening 23 for filtering is provided on the support platform 21, a plug connecting rod 6 for blocking the discharge pipe opening 32 is rotatably provided at the center of the support platform 21 facing the discharge pipe opening 32 through a bearing 5, the bearing 5 is interference fit with the plug connecting rod 6, a plug 61 matching the discharge pipe opening 32 is provided at the lower end of the plug connecting rod 6, the upper end of the receiving tube 4 is coaxially sleeved on the outer wall of the liquid dispensing tube 3 through a detachable sealing connection structure 7, and a cavity for receiving liquid is formed in the receiving tube 4 between the lower end of the liquid dispensing tube 3 and the bottom of the receiving tube 4.

[0028] The support platform 21 is constructed by a plurality of support rods 24 integrally connected to the inner wall of the filter short tube 2, and the plurality of support rods 24 are arranged radially along the filter short tube 2. The center of the filter short tube 2 is integrally connected with a vertical short tube 25 through the support rods 24, and the upper end of the vertical short tube 25 is flush with the upper end of the filter short tube 2. The plug connecting rod 6 is rotatably mounted on the vertical short tube 25 through a bearing 5, and an opening 23 for filtering is formed between two adjacent support rods 24.

[0029] The detachable sealing connection structure 7 is a slot connection structure 71, which can play a role in sealing connection and positioning. The slot connection structure 71 includes a sealing ring 1 72 integrally connected to the outer wall of the dispensing tube 3, and the bottom of the sealing ring 1 72 is integrally connected to a latch tongue 73. The top outer wall of the receiving tube 4 is integrally connected with a sealing ring 2 74. The sealing ring 2 74 is provided with a slot 75 corresponding to the position of the latch tongue 73, and the dispensing tube 3 and the receiving tube 4 can be quickly plugged into the slot 75 and the latch tongue 73 to achieve a sealed sleeve connection.

[0030] The plug 61 is an ellipsoidal or nearly conical rubber plug that matches the shape of the conical bottom 31 of the dispensing tube 3. The side wall of the rubber plug is designed to be arc-shaped. The discharge pipe outlet 32 ​​is opened at the lower end of the conical bottom 31 of the dispensing tube 3 near the top angle. The arc-shaped design of the side wall of the rubber plug can reduce the contact area between the side wall of the plug 61 and the conical bottom 31 during sealing, thereby reducing the pressure to achieve sealing and the pulling force required to pull up the plug 61 during drainage.

[0031] The conical bottom 31 of the dispensing tube 3 is a pointed cone with a taper of not less than 75 degrees. When draining liquid from the bottom, due to the small volume of the bottom of the pointed cone, less liquid can be accommodated. When the plug 61 is pulled up to drain liquid, the disturbance of the liquid in the dispensing tube 3 can be reduced, and it is convenient to observe the progress of liquid separation, and seal it in time when the liquid separation is completed. A sealing structure 33 is provided between the discharge pipe mouth 32 and the plug 61 to prevent leakage. The sealing structure 33 includes an annular groove for installing a sealing ring arranged on the bottom end of the conical bottom 31 or the discharge pipe mouth 32, and an elastic sealing ring is installed in the annular groove.

[0032] The liquid dispensing tube 3 is provided with a limiting structure 34 to prevent the plug 61 and the liquid discharge pipe opening 32 from being blocked too tightly.

[0033] When the utility model is used, the upper and lower ends of the filtering short tube 2 are connected to the sealing cover 1 and the upper end of the liquid dispensing tube 3 respectively through a threaded connection structure. When in use, the sealing cover 1 is opened, and the filtering short tube 2 is screwed until the plug at the lower end of the plug connecting rod 6 is downwardly plugged to block the discharge pipe opening 32 to prevent liquid leakage. The sample is injected into the liquid dispensing tube 3 through the opening 23 arranged on the supporting platform 21, and the sample is filtered through the filter membrane on the supporting platform 21. The upper end of the receiving tube 4 is connected to the outer wall of the liquid dispensing tube 3 through a detachable sealing connection structure 7. When the sample needs to be separated after centrifugation and stratification, the reverse rotation is performed. When the filter short tube 2 is screwed, the plug connecting rod 6 moves upward with the screwing of the filter short tube 2. The plug 61 at the lower end of the plug connecting rod 6 is separated from the discharge pipe port 32 under the action of tension, and the liquid is directly discharged into the receiving pipe 4 from the discharge pipe port 32. Since the plug connecting rod 6 is rotatably arranged at the center of the support platform 21 through the bearing 5, when the filter short tube 2 is screwed, the plug connecting rod 6 will not rotate with the rotation of the filter short tube 2, thereby reducing the disturbance to the liquid. At the same time, since the lifting and lowering of the plug connecting rod 6 is controlled by screwing the filter short tube, it is convenient to control the lifting and lowering speed and amplitude of the plug 61, thereby avoiding severe disturbance to the liquid during plugging or draining.

[0034] Example 2, reference Figure 6, which is different from Example 1 in that a conical tube 35 is integrally connected to the drainage pipe opening 32, the small end of the conical tube 35 extends downward from the drainage pipe opening 32, the large end of the conical tube 35 is higher than the lower end of the conical bottom 31, the side wall of the conical tube 35 is provided with a plurality of drainage holes 36, the bottoms of the plurality of drainage holes 36 are flush with the bottom of the conical bottom 31, the side wall of the plug 61 is adapted to the inner wall of the conical tube 35, and since the conical tube 35 is much smaller than the conical bottom 31 of the liquid dispensing tube 3, the inner wall of the conical tube 35 is aligned with the side wall of the plug 61 The wall cooperates to achieve sealing of the liquid, and the disturbance to the liquid in the dispensing tube 3 is small. The design of the discharge hole 36 prevents the liquid from flowing too fast during discharge and forming a vortex at the discharge pipe mouth, thereby better avoiding liquid disturbance. The filter short tube 2 is provided with a butterfly knob 26 for easy knob rotation, and the sealing cover 1 is provided with a vent pipe mouth 11, and a sealing pipe cap is provided on the vent pipe mouth 11. When negative pressure occurs in the dispensing tube 3 due to liquid discharge and affects the discharge speed, there is no need to open the sealing cover 1, and air can be released through the vent pipe mouth 11 to make the discharge smoother.

Claims

1. A centrifuge tube for detecting alkaloids in health food, characterized in that: The filter cartridge has a plurality of zippered ends that are adapted to engage with the filter element and to engage with the filter element in a manner that allows the filter element to pass through the filter element and to engage with the filter element in a manner that allows the filter element to pass through the filter element in a manner that is compatible with the filter element.

2. A centrifuge tube for detecting alkaloids in health food according to claim 1, characterized in that: The support platform is constructed by multiple support rods integrally connected to the inner wall of the filter short tube, the multiple support rods are arranged radially along the filter short tube, the center of the filter short tube is integrally connected with a vertical short tube through the support rods, the upper end of the vertical short tube is flush with the upper end of the filter short tube, the plug connecting rod is rotatably mounted on the vertical short tube through a bearing, and an opening for filtering is formed between two adjacent support rods.

3. A centrifuge tube for detecting alkaloids in health food according to claim 1 or 2, characterized in that: The detachable sealing connection structure is a slot connection structure, which can play a role of sealing connection and positioning.

4. A centrifuge tube for detecting alkaloids in health food according to claim 3, characterized in that: The slot connection structure includes a sealing ring 1 integrally connected to the outer wall of the dispensing tube, the bottom of the sealing ring 1 is integrally connected to a latch tongue, the top outer wall of the receiving tube is integrally connected to a sealing ring 2, and a slot matching the latch tongue is arranged on the sealing ring 2 corresponding to the position of the latch tongue.

5. The centrifuge tube for detecting alkaloids in health food according to claim 1, characterized in that: The plug head is an ellipsoidal or nearly conical rubber plug that matches the conical bottom shape of the dispensing tube. The side wall of the rubber plug is designed to be arc-shaped, and the discharge pipe opening is opened at the lower end of the conical bottom of the dispensing tube near the top angle.

6. A centrifuge tube for detecting alkaloids in health food according to claim 5, characterized in that: The conical bottom of the liquid dispensing tube is a pointed cone with a taper of not less than 75 degrees.

7. A centrifuge tube for detecting alkaloids in health food according to claim 6, characterized in that: A sealing structure for preventing leakage is arranged between the discharge pipe opening and the plug head. The sealing structure comprises an annular groove for installing a sealing ring arranged at the bottom end of the conical bottom or on the discharge pipe opening, and an elastic sealing ring is installed in the annular groove.

8. The centrifuge tube for detecting alkaloids in health food according to claim 1, characterized in that: A conical tube is integrally connected to the drainage pipe outlet, the small end of the conical tube extends downward from the drainage pipe outlet, the large end of the conical tube is higher than the lower end of the conical bottom, a plurality of drainage holes are arranged on the side wall of the conical tube, the bottoms of the plurality of drainage holes are flush with the bottom of the conical bottom, and the side wall of the plug is adapted to the inner wall of the conical tube.

9. A centrifuge tube for detecting alkaloids in health food according to claim 1, characterized in that: The filtering short tube is provided with a butterfly-shaped knob for easy turning.

10. A centrifuge tube for detecting alkaloids in health food according to claim 1, characterized in that: The liquid dispensing tube is provided with a limiting structure to prevent the plug and the liquid discharge pipe opening from being blocked too tightly.

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