Solution transfer and storage device and immunohistochemical instrument

Abstract

The utility model relates to a solution transfer and storage device and an immunohistochemical instrument, and relates to the technical field of immunohistochemical instruments, the solution transfer and storage device comprises a bottle body, the bottle body is cylindrical, and a liquid outlet is formed in the lower portion of the bottle body; the probe assembly can be inserted into the bottle body and is used for detecting liquid level information in the bottle body; the liquid inlet rod is inserted into the bottle body along the direction towards the central axis of the bottle body; a liquid inlet is formed in the end face, located outside the bottle body, of the liquid inlet rod, one end face, located in the bottle body, of the liquid inlet rod is blocked, and a communicating hole is formed in the side wall of the end, located in the bottle body, of the liquid inlet rod and enables the bottle body to enter the bottle body from the liquid inlet in the side wall direction of the bottle body. The problem that in the related technology, floating ball type measurement is prone to being influenced by buoyancy or ultrasonic measurement is prone to being influenced by pressure and water mist, and consequently the liquid level measurement precision is not high is solved.

Description

Technical Field

[0001] The present application relates to the technical field of immunohistochemical analyzers, and in particular to a solution transfer storage device and an immunohistochemical analyzer. Background Art

[0002] Immunohistochemistry is the application of the basic principle of immunology - antigen-antibody reaction, that is, the principle of specific binding of antigen and antibody. It uses chemical reaction to make the color developer (fluorescein, enzyme, metal ion, isotope) labeled with antibody develop color to determine the antigen (peptide and protein) in tissue cells, and conduct localization, qualitative and relative quantitative research on it. This is called immunohistochemistry or immunocytochemistry.

[0003] Because immunohistochemistry has the characteristics of strong specificity, high sensitivity, accurate positioning, and can organically combine morphological research with functional research, this new technology has been widely used in many fields of biological and medical research. In pathological research, the role and significance of immunohistochemistry technology are even more important.

[0004] If you want an automated device to be used for immunohistochemistry, a large amount of reagents will inevitably be used. The corresponding reagents may need to be transported to the tissue sample slide to be analyzed, or they may be reagent waste liquid recovered after a chemical reaction on the slide with the sample. The automated equipment needs to realize the delivery or recovery of reagents. In order to meet the needs of batch and automated continuous operations, the amount of reagent delivery and recovery liquid needs to be controlled. Liquid level monitoring must be implemented, but existing liquid level monitoring, such as float measurement or ultrasonic measurement, will be inaccurate due to uncertain reagent density or the presence of water mist.

[0005] Therefore, there is an urgent need for a reagent storage or delivery device that can be applied to this special scenario and can realize a high-precision liquid volume detection function. Utility Model Content

[0006] The purpose of the present application is to provide a solution transfer storage device for solving the problem in the related art that float ball measurement is easily affected by buoyancy or ultrasonic measurement is easily affected by pressure and water mist, resulting in low liquid level measurement accuracy.

[0007] In the first aspect, the present application provides a solution transfer storage device adopting the following technical solution:

[0008] A solution transfer storage device, comprising

[0009] The bottle body is cylindrical and has a liquid outlet at the bottom.

[0010] A probe assembly, which can be inserted into the bottle body and is used to detect liquid level information in the bottle body;

[0011] A liquid inlet rod, the liquid inlet rod being inserted into the bottle body along a direction toward the central axis of the bottle body;

[0012] The liquid inlet rod is provided with a liquid inlet on the end surface outside the bottle body, and a connecting hole is provided at one end inside the bottle body, and the connecting hole allows liquid to enter the bottle body from the liquid inlet along the side wall of the bottle body.

[0013] By adopting the above technical solution, the reagent enters the bottle body through the liquid inlet rod, and the probe assembly suspended in the bottle body can measure the liquid level height of the reagent.

[0014] The liquid inlet rod allows the liquid to enter the bottle body from the liquid inlet along the side wall of the bottle body, the liquid flows out horizontally along the inner wall of the cylindrical cross section, and finally flows in a spiral along the inner wall. At this time, the reagent can enter the bottle body more smoothly from the connecting hole.

[0015] In immunohistochemistry technology, a large amount of reagents are used. The corresponding reagents are transported to the tissue sample slide to be analyzed, or the reagent waste liquid that undergoes chemical reactions on the slide with the sample is recovered. Therefore, automated equipment needs to realize the transportation or recovery of reagents. In order to meet the needs of batch and automated continuous operations, the amount of liquid transported and recovered is also required to be controlled. Liquid level monitoring must be achieved, and the probe assembly must be inserted into the bottle to reduce the direct contact between the liquid and the probe or the impact of the incoming liquid on the stability of the liquid surface. Accurate liquid level detection can be achieved to solve the problem that float measurement is easily affected by buoyancy or ultrasonic measurement is easily affected by pressure and water mist, resulting in low liquid level measurement accuracy.

[0016] Optionally, the liquid inlet rod is a tubular structure, and the direction of the connecting hole is perpendicular to the radial direction of the bottle body.

[0017] By adopting the above technical solution, the liquid flows out horizontally along the inner wall of the cylindrical cross section and finally flows in a spiral along the inner wall. The reagent enters the bottle body smoothly, preventing interference with the accuracy of liquid level detection.

[0018] Optionally, there are two communicating holes, and the two communicating holes are horizontally arranged opposite to each other.

[0019] By adopting the above technical solution, the liquid inlet flow rate is guaranteed while ensuring that the reagent enters the bottle body smoothly, thereby preventing interference with the liquid level detection accuracy.

[0020] Optionally, reinforcing ribs are provided on the side walls of the bottle body.

[0021] By adopting the above technical solution, reinforcing ribs are provided on the side walls of the bottle body, and the reinforcing ribs can ensure that the internal volume of the bottle body does not change under the working pressure, and then a probe is used for liquid level detection, and the conversion of reagent liquid volume is accurate.

[0022] Optionally, a connecting tube is provided on the bottle body, the connecting tube is connected to the bottle body, and a first limiting piece is provided on the connecting tube; a second limiting piece is provided on the liquid inlet rod, and the liquid inlet rod is circumferentially limited and inserted in the connecting tube through the cooperation of the first limiting piece and the second limiting piece.

[0023] By adopting the above technical solution, the first limit member and the second limit member cooperate to limit, thereby preventing installation misalignment or circumferential rotation of the liquid inlet rod along the central axis, so that when the liquid inlet rod is inserted into the connecting tube, the relative position of the connecting hole in the bottle body is fixed, and the liquid always enters the bottle body from the liquid inlet along the cross-sectional direction of the side wall of the bottle body, thereby avoiding interference with the probe and ensuring the accuracy of liquid level detection.

[0024] Optionally, the probe assembly includes a probe seat and a probe, the probe seat is arranged at the bottle mouth of the bottle body, the probe is fixedly connected to the probe seat, a protective plate is arranged on the probe seat, the protective plate is located between the probe and the liquid inlet rod, and is used to block the liquid inlet disturbance in the direction of the liquid inlet rod.

[0025] By adopting the above technical solution, the protection plate is located on the side close to the liquid inlet rod, playing the role of secondary protection of the probe, preventing the liquid caused by the liquid inlet rod from splashing onto the probe.

[0026] Optionally, the protection plate is an arc protection plate.

[0027] By adopting the above technical solution, it is prevented that the pressure inside and outside the arc protection plate is inconsistent, resulting in the liquid levels on both sides of the arc protection plate being at different heights, thereby affecting the accuracy of the probe's liquid level detection.

[0028] Optionally, a third limiter is provided on the probe seat, and a fourth limiter is provided on the bottle body. The probe seat is inserted into the bottle body in a circumferentially limited manner through the cooperation of the third limiter and the fourth limiter, and the protective plate is located on the side that blocks the liquid inlet rod from inletting liquid.

[0029] By adopting the above technical solution, the probe seat is circumferentially limited to the bottle body by the clamping connection between the third limiter and the fourth limiter, and will not be installed misaligned during the installation process, and the probe seat will not move circumferentially, ensuring that the protective plate is always located on the side that blocks the liquid inlet rod from entering the liquid.

[0030] Optionally, a liquid level detection plate is further included, wherein the liquid level detection plate is located at the top of the probe base body, and the probe passes through the probe base body and is in electrical contact with the liquid level detection plate.

[0031] By adopting the above technical solution, the liquid level detection board and the probe are installed in direct contact, which can reduce additional cable connections, reduce inherent capacitance, and improve the accuracy of capacitive liquid level detection of the probe.

[0032] Optionally, the number of the probes is at least three, namely a first probe, a second probe and a third probe, and the depths of the first probe, the second probe and the third probe inserted into the bottle body increase in sequence;

[0033] By adopting the above technical solution, the probe assembly can accurately obtain at least three different liquid level heights in the bottle body. When the solution transfer storage device is used in automated equipment, it can flexibly realize continuous and efficient operation of the equipment.

[0034] Optionally, a waterproof membrane structure is provided on one side of the probe base body facing the inside of the bottle body to form a plurality of grooves spaced apart from each other, and the probes correspond to the grooves one by one and are located in the corresponding grooves.

[0035] By adopting the above technical solution, multiple grooves are arranged at intervals from each other, which can prevent the probes connected to the probe seat from being conducted by the water film, resulting in electrical connection between multiple probes, affecting the liquid level detection board's detection of capacitance changes, thereby further ensuring the accuracy of the probe liquid level detection.

[0036] In the second aspect, the present application provides a solution transfer storage device adopting the following technical solution:

[0037] An immunohistochemical instrument comprises a solution transfer storage device.

[0038] By adopting the above technical solution, the corresponding immunohistochemical instrument can contain one, two or more solution transfer storage devices. When there is only one, the solution transfer storage device is used for reagent extraction or waste liquid collection.

[0039] In summary, the present application includes at least one of the following beneficial technical effects:

[0040] 1. The density of the liquid to be monitored is uncertain, and the automation equipment has high requirements for liquid level accuracy. This solution adopts a capacitive liquid level detection solution and inserts the probe assembly into the bottle body, avoiding the problem that the float measurement is greatly affected by the density of the liquid to be measured or the ultrasonic detection is easily affected by pressure and water mist, resulting in low liquid level measurement accuracy; at the same time, through the special design of the liquid inlet rod, the impact of liquid entry on the probe is reduced, which can further accurately detect the liquid level.

[0041] 2. In the two different application scenarios of reagent extraction transfer and waste liquid collection, the usage and functions of the three probes are different. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 This is a schematic diagram of the structure of an embodiment of the present application;

[0043] Figure 2It is a cross-sectional schematic diagram of the first viewing angle of the embodiment of the present application;

[0044] Figure 3 It is a cross-sectional schematic diagram of the second viewing angle of the embodiment of the present application;

[0045] Figure 4 for Figure 3 A partial enlarged schematic diagram of part A;

[0046] Figure 5 for Figure 3 A partial enlarged schematic diagram of part B.

[0047] In the figure:

[0048] 10. Bottle body; 11. Connecting pipe; 111. First limiting member; 1111. First limiting groove; 12. Fourth limiting member; 121. Second limiting groove; 13. Reinforcing rib; 14. Liquid discharge port; 15. Vent port; 16. Scale groove;

[0049] 20. Probe assembly; 21. Probe seat; 211. Protective plate; 212. Third stopper; 2121. Second stopper; 2122. Second sealing boss; 213. Groove; 22. Probe; 221. First probe; 222. Second probe; 223. Third probe;

[0050] 30, liquid inlet rod; 31, liquid inlet; 32, connecting hole; 33, second limiting member; 331, first limiting block; 332, first sealing boss;

[0051] 40. Liquid level detection board;

[0052] 50. Bottle cover;

[0053] 60. Waterproof cover;

[0054] 70. Threaded pipe;

[0055] 80. Sealing gasket;

[0056] 90. Sealing ring. DETAILED DESCRIPTION

[0057] The following is combined with Figure 1 -Attached Figure 5 , further details of this application are given.

[0058] The embodiment of the present application discloses a solution transfer storage device.

[0059] Example 1

[0060] Reference Figure 1 and Figure 2A solution transfer storage device includes a bottle body 10, a probe assembly 20, a liquid inlet rod 30, a liquid level detection plate 40, a bottle sleeve 50 and a waterproof cover 60. The probe assembly 20 is suspended in the bottle body 10, and the probe assembly 20 can be inserted into the bottle body 10 and can detect the liquid level information in the bottle body 10. The bottle body 10 is provided with a drain port 14 and a vent 15. The liquid reagent is discharged from the bottle body 10 through the drain port 14. The vent 15 can connect the bottle body 10 with an air pump, so that the bottle body 10 is in a positive pressure or negative pressure environment.

[0061] Combination Figure 1 and Figure 2 The liquid inlet rod 30 is located on the side of the bottle body 10. The liquid inlet rod 30 is inserted into the bottle body 10 along a direction perpendicular to the central axis of the bottle body 10 and is connected to the bottle body 10. A liquid inlet port 31 is provided on the end surface of the liquid inlet rod 30 located outside the bottle body 10. The end of the liquid inlet rod 30 located inside the bottle body 10 is a closed end. A connecting hole 32 is provided on the side wall of the end of the liquid inlet rod 30 located inside the bottle body 10. The direction of the connecting hole 32 is along the radial direction perpendicular to the bottle body 10. There are two connecting holes 32, and the two connecting holes 32 are horizontally arranged opposite to each other.

[0062] like Figure 2 The reagent enters the bottle body 10 through the liquid inlet rod 30. The liquid inlet rod 30 is set higher than the highest liquid level in the bottle body 10. The probe assembly 20 suspended in the bottle body 10 can measure the liquid level of the reagent. This makes it easy to extract liquid by forming a negative pressure in the bottle body 10. At the same time, when the bottle body 10 is used for collecting, storing or discharging waste liquid, the waste liquid will not flow into the liquid inlet rod 30 and the pipeline or pump body connected thereto, causing pipeline blockage or even damage to the pump body.

[0063] The liquid inlet rod 30 can be inserted horizontally in the bottle body 10, and the reagent enters the liquid inlet rod 30 from the liquid inlet port 31 outside the liquid inlet rod 30. The liquid inlet rod 30 is blocked at one end in the bottle body 10, which can prevent the reagent entering from the liquid inlet rod 30 from being sprayed and splashed onto the probe assembly 20 suspended in the bottle body 10 or directly sprayed onto the liquid surface in the bottle body 10, causing huge fluctuations in the liquid surface. The reagent impact is too large to cause the vibration of the probe 22 or the vibration of the liquid surface, so that the probe 22 interferes with the detection accuracy of the liquid level in the bottle. At the same time, the connecting hole 32 is connected to the liquid inlet port 31 and the bottle body 10 respectively, so that when the reagent entering from the liquid inlet rod 30 is blocked by the closed end of the liquid inlet rod 30, the reagent flows out from the two horizontally arranged connecting holes 32, and the liquid flows out horizontally along the inner wall of the cylindrical cross section, and finally flows along the inner wall in a spiral. At this time, the reagent can enter the bottle body 10 relatively smoothly from the connecting hole 32.

[0064] In immunohistochemistry technology, a large amount of reagents are used to transport the corresponding reagents to the tissue sample slide to be analyzed, or to recover the reagent waste liquid that has undergone chemical reactions on the slide with the sample. Therefore, the automated equipment needs to realize the transportation or recovery of the reagents. In order to meet the needs of batch and automated continuous operations, the amount of liquid transported and recovered is also required to be controlled, and liquid level monitoring must be realized. The density of the liquid to be monitored is uncertain, and the automated equipment has high requirements for liquid level accuracy. This scheme adopts a capacitive liquid level detection scheme, and the probe assembly 20 is inserted into the bottle body 10, avoiding the problem that the float type measurement is greatly affected by the density of the liquid to be measured or the ultrasonic detection is easily affected by pressure and water mist, resulting in low liquid level measurement accuracy; at the same time, through the special design of the liquid inlet rod 30, the impact of liquid entry on the probe 22 is reduced, which can further accurately detect the liquid level.

[0065] Reference Figure 3 and Figure 4 A connecting pipe 11 is provided on the bottle body 10, and the connecting pipe 11 is connected to the bottle body 10. The liquid inlet rod 30 is located in the connecting pipe 11 and inserted in the connecting pipe 11. A first limiting member 111 is provided on the connecting pipe 11, and a second limiting member 33 is provided on the peripheral side of the liquid inlet rod 30, and the second limiting member 33 can abut against the end of the connecting pipe 11; the first limiting member 111 and the second limiting member 33 cooperate to limit, so as to prevent the installation from being misplaced, or the liquid inlet rod 30 from rotating circumferentially along the central axis; in addition, the length of the liquid inlet rod 30 matches the length of the connecting pipe 11, so that after the liquid inlet rod 30 axially passes through the wall of the bottle body 10, the connecting hole 32 is just located near the inner wall of the bottle body 10. This structure ensures that when the liquid inlet rod 30 is inserted into the connecting tube 11, the connecting hole 32 is relatively fixed in the bottle body 10. When liquid enters from the liquid inlet 31, it will always flow in a spiral along the inner wall of the bottle body 10 and then smoothly enter the bottle body 10 without interfering with the probe 22, thereby ensuring the accuracy of liquid level detection.

[0066] In this embodiment, the first limiting member 111 is a first limiting groove 1111, and the second limiting member 33 is a first limiting block 331, and the first limiting block 331 is engaged with the first limiting groove 1111 to prevent the liquid inlet rod 30 from rotating in the circumferential direction. In other embodiments, the first limiting member 111 may be a limiting block, and the second limiting member 33 may be a limiting groove, and the limiting block is engaged with the limiting groove to prevent the liquid inlet rod 30 from rotating in the circumferential direction.

[0067] Furthermore, a first sealing boss 332 is provided on the liquid inlet rod 30, and the first sealing boss 332 abuts against the free end of the connecting tube 11; the bottle body 10 is also provided with a threaded tube 70 and a sealing gasket 80 during use, and the closed end of the liquid inlet rod 30 is inserted into the connecting tube 11, and the liquid inlet rod 30 is limited by the connecting tube 11 in the circumferential direction and will not move in the circumferential direction. After the liquid inlet rod 30 is inserted into the connecting tube 11 until the connecting hole 32 is connected to the bottle body 10, the first sealing boss 332 abuts against the free end of the connecting tube 11. 32 abuts against the end of the connecting tube 11 through the sealing gasket 80, limits the axial side of the liquid inlet rod 30, and prevents the liquid inlet rod 30 from extending too long or too short into the bottle body 10, so that after the liquid inlet rod 30 axially passes through the wall of the bottle body 10, the connecting hole 32 is just located near the inner wall of the bottle body 10. When the liquid enters from the liquid inlet 31, it always flows in a spiral along the inner wall of the bottle body 10 and then smoothly enters the bottle body 10. At the same time, the sealing gasket 80 plays a certain sealing role.

[0068] The threaded tube 70 is sleeved on the connecting tube 11 and the first sealing boss 332 . The inner side of the threaded tube 70 is provided with a step surface, which abuts against the end of the first sealing boss 332 . The outer side of the connecting tube 11 is provided with threads, and the threaded tube 70 can be threadedly connected to the connecting tube 11 .

[0069] When the liquid inlet rod 30 is inserted into the connecting tube 11 and the first sealing boss 332 abuts against the end of the connecting tube 11, the threaded tube 70 is rotatably sleeved on the outside of the connecting tube 11 and the first sealing boss 332, and the threaded tube 70 is threadedly connected with the outside of the connecting tube 11, and the threaded tube 70 is rotated until the step surface inside the threaded tube 70 abuts against the first sealing boss 332. Thus, the threaded tube 70 abuts against the first sealing boss 332, the liquid inlet rod 30 is axially limited, and the liquid inlet rod 30 is fixed relative to the bottle body 10.

[0070] Reference Figure 2 and Figure 3 The specific structure of the probe assembly 20 and the specific connection relationship between the probe assembly 20 and the bottle body 10 are as follows: the probe assembly 20 includes a probe seat body 21 and a probe 22, the probe 22 penetrates the probe seat body 21 and is fixedly connected to the probe seat body 21, the probe seat body 21 and the probe 22 can be inserted into the bottle mouth at the top of the bottle body 10, a protective plate 211 is arranged on the probe seat body 21, the protective plate 211 is suspended inside the bottle body 10, and the protective plate 211 is used to block the liquid inlet disturbance in the direction of the liquid inlet rod 30.

[0071] The probe base 21 is inserted into the bottle body 10 and abuts against the bottle mouth. The probe 22 is suspended in the bottle body 10 and can detect the liquid level. The protection plate 211 is located on the side close to the liquid inlet rod 30, and plays the role of secondary protection for the probe 22. It can prevent liquid splashing caused by liquid inflow into the liquid inlet rod 30 from interfering with the liquid level detection of the probe 22, thereby affecting the liquid level detection accuracy.

[0072] Reference Figure 2 and Figure 3 The protection plate 211 is in the shape of an arc plate. The arc plate shape makes the liquid level outside the plate and inside the plate at the same height, preventing the situation where the pressure inside and outside the arc is inconsistent, resulting in the liquid level inside and outside the protection plate 211 being at different heights, thereby affecting the detection of the liquid level by the probe 22; at the same time, the arc plate shape can facilitate the observation of the liquid level and facilitate the cleaning and maintenance of the probe assembly 20.

[0073] Reference Figure 3 and Figure 5 The probe seat 21 is provided with a third stopper 212 on the circumferential side, and the bottle body 10 is provided with a fourth stopper 12. The third stopper 212 is engaged with the fourth stopper 12, and the circumferential stopper is inserted in the bottle body 10. When the probe assembly 20 is inserted, the third stopper 212 is engaged with the fourth stopper 12, and the protection plate 211 is located on the side that blocks the liquid inlet rod 30 from entering the liquid. The bottle sleeve 50 is located outside the bottle body 10 and the probe seat 21. The bottle sleeve 50 is sleeved on the outer periphery of the probe seat 21 and is threadedly connected with the outer periphery of the bottle body 10. The waterproof cover 60 is located above the bottle sleeve 50, and the waterproof cover 60 is covered on the bottle sleeve 50.

[0074] In this embodiment, the fourth limiting member 12 is the second limiting groove 121, the third limiting member 212 is the second limiting block 2121, and the second limiting block 2121 is engaged with the second limiting groove 121 to prevent the probe seat body 21 from rotating in the circumferential direction; so that the installation will not be misplaced during the installation process, and the probe seat body 21 will not move in the circumferential direction, ensuring that the protective plate 211 is always located on the side that blocks the liquid inlet rod 30 from entering the liquid. In other embodiments, it is also possible that the fourth limiting member 12 is a limiting block, the third limiting member 212 is a limiting groove, and the limiting block is engaged with the limiting groove to prevent the probe seat body 21 from rotating in the circumferential direction.

[0075] The probe seat 21 is also provided with a second sealing boss 2122, which abuts against the free end of the bottle mouth of the bottle body 10. The probe seat 21 is inserted into the bottle body 10, and the second sealing boss 2122 abuts against the bottle mouth of the bottle body 10, which limits the axial position of the probe seat 21 to prevent the probe seat 21 from falling into the bottle body 10. Specifically, the bottle sleeve 50 is sleeved on the outside of the bottle body 10 and the probe seat 21, and the inner side of the bottle sleeve 50 is provided with a step surface, which abuts against the end of the second sealing boss 2122; the outer side of the bottle mouth of the bottle body 10 is provided with a thread, and the bottle sleeve 50 can be threadedly connected with the bottle body 10, and the waterproof cover 60 is covered on the bottle sleeve 50, which can protect the liquid level detection plate 40 and prevent water from penetrating into and damaging the liquid level detection plate 40.

[0076] When the probe base 21 is inserted into the bottle body 10 and the second sealing boss 2122 of the probe base 21 is in contact with the bottle body 10, the bottle sleeve 50 is rotated and sleeved on the outside of the bottle body 10 and the probe base 21, and the bottle sleeve 50 is threadedly connected with the outside of the bottle body 10. The bottle sleeve 50 is rotated until the step surface inside the bottle sleeve 50 is in contact with the second sealing boss 2122 through the sealing ring 90, and the sealing ring 90 plays a sealing role. In this way, the bottle sleeve 50 is in contact with the probe base 21, the probe assembly 20 is axially limited, and the probe assembly 20 is fixed relative to the bottle body 10.

[0077] Reference Figure 2 and Figure 3 The probe 22 includes a first probe 221, a second probe 222 and a third probe 223. The lengths of the first probe 221, the second probe 222 and the third probe 223 increase successively, and the depths of the probes inserted into the bottle body 10 increase successively. The detection point of the third probe 223 corresponds to the minimum liquid level height monitored, the detection point of the second probe 222 corresponds to the maximum liquid level height monitored, and the detection point of the first probe 221 corresponds to the warning liquid level height.

[0078] The bottle 10 can be used normally for liquid storage only when the liquid level reaches the height detectable by the third probe 223 and does not exceed the height detectable by the second probe 222. When the second probe 222 fails, the first probe 221 can prevent the liquid from continuously flowing into the liquid inlet rod 30 and causing the liquid level to be too high and flow back into the vent 15.

[0079] Reference Figure 2 and Figure 3 The liquid level detection board 40 is located between the bottle sleeve 50 and the probe seat 21, and the liquid level detection board 40 is fixedly connected to the probe 22. The liquid level detection is detected by capacitance change, which is not affected by different liquid concentrations and densities, so that the detection result is only related to the liquid level height; the liquid level detection board 40 and the probe 22 are directly installed in contact, which can reduce additional cable connections, reduce inherent capacitance, and improve the accuracy of the capacitive liquid level detection device such as the probe 22.

[0080] Reference Figure 2 and Figure 3 A waterproof membrane structure is provided on one side of the probe seat 21 facing the inside of the bottle body 10 to form three mutually spaced grooves 213 , each probe 22 corresponds to a groove 213 , the side wall edges of the grooves 213 are inclined, and sharp portions are formed between adjacent grooves 213 .

[0081] The reagent liquid in the bottle body 10 may form a water film at the bottom of the probe seat body 21. The three grooves 213 are arranged at intervals from each other to prevent the probes 22 connected to the probe seat body 21 from being conducted by the water film, resulting in electrical connection between the three probes 22, affecting the detection of capacitance changes by the liquid level detection plate 40.

[0082] The inclined edge of the side wall of the groove 213 can make the liquid drip along the sharp edges when liquid appears on the top, thereby preventing excessive accumulation of liquid from accidentally touching the probe 22 and affecting the measurement accuracy of the liquid level.

[0083] Reference Figure 1 and Figure 2 The bottle body 10 is provided with reinforcing ribs 13, which can ensure that the internal volume of the bottle body 10 does not change under the working pressure. When the probe 22 is used for liquid level detection, the accuracy of converting the reagent liquid volume is high.

[0084] Reference Figure 1 A graduated groove 16 is provided on the bottle body 10, and the graduated groove 16 can be set to 300ml, 500ml and 800ml, corresponding to the third probe 223, the second probe 222 and the first probe 221 respectively, so as to directly observe the liquid level.

[0085] The implementation principle of a solution transfer storage device in this embodiment is as follows: the bottle body 10 is arranged in a cylindrical shape, and is provided with a connecting pipe 11 located at the liquid inlet end, a liquid discharge port 14 and a vent 15 on the bottle body 10; the probe seat body 21 and the protective plate 211 in the probe assembly 20 are integrally formed; the probe 22 is fixedly connected to the probe seat body 21; the probe seat body 21 is inserted into the bottle body 10 and abuts against the upper end of the bottle body 10; the protective plate 211 and the probe 22 extend into the bottle body 10 together. The liquid inlet rod 30 is inserted into the bottle body 10 and abuts against the end of the connecting tube 11. A liquid inlet port 31 is provided on the end surface of the liquid inlet rod 30 outside the bottle body 10. One end located inside the bottle body 10 is a closed end, and a connecting hole 32 is provided on the side wall of the closed end. The two connecting holes 32 are horizontally arranged in a radial direction perpendicular to the bottle body 10. The reagent flows out from the two horizontally arranged connecting holes 32, and the liquid flows out horizontally along the inner wall of the cylindrical cross section and finally flows in a spiral along the inner wall. At this time, the reagent can enter the bottle body 10 relatively smoothly from the connecting hole 32, thereby avoiding the liquid inlet liquid directly hitting the probe 22 or impacting the liquid surface to cause large fluctuations in the liquid surface, which affects the accuracy of the probe 22 in detecting the liquid level height.

[0086] The protection plate 211 inserted into the bottle body 10 is set as an arc plate. Through the circumferential limitation of the second limit block 2121 and the second limit groove 121, when the probe seat 21 is inserted into the bottle body 10, the protection plate 211 is always located on the side of the liquid inlet rod 30, which plays a secondary protection role for the probe 22 and prevents the liquid from entering the bottle body 10 and affecting the detection accuracy of the probe 22.

[0087] Inserting the probe assembly 20 into the bottle body 10 and reducing the impact of the incoming liquid on the probe 22 or the impact of the incoming liquid on the liquid surface can achieve accurate liquid level detection and solve the problem that float measurement is easily affected by buoyancy or ultrasonic measurement is easily affected by pressure and water mist, resulting in low liquid level measurement accuracy.

[0088] The reagent liquid in the bottle body 10 may form a water film at the bottom of the probe seat body 21. The three grooves 213 are arranged at intervals from each other to prevent the probes 22 connected to the probe seat body 21 from being conducted by the water film, resulting in electrical connection between the three probes 22, affecting the detection of capacitance changes by the liquid level detection plate 40.

[0089] The probe assembly 20 is inserted into the bottle body 10 and reduces the direct contact of the incoming liquid and the hanging liquid on the probe 22 or the influence of the incoming liquid on the liquid surface stability, so that accurate liquid level detection can be achieved.

[0090] Example 2

[0091] An immunohistochemical instrument, the difference between this embodiment and embodiment 1 is that the liquid inlet rod 30 can be L-shaped or T-shaped.

[0092] The L-shaped liquid inlet rod 30 can be formed by two straight tubes connected, one of which is inserted toward the central axis of the bottle body 10, and the other straight tube is along the radial direction perpendicular to the bottle body 10; the L-shaped liquid inlet rod 30 can also be formed by a straight tube and an arc tube connected to each other, the straight tube is inserted toward the central axis of the bottle body 10, and the other arc-shaped curved tube is transversely along the wall of the bottle body 10, and its curvature is consistent with the transverse curvature of the wall. The straight tube of the L-shaped liquid inlet rod 30 is provided with a liquid inlet 31 on the end surface outside the bottle body 10, and the end of the straight tube inserted toward the central axis of the bottle body 10 located inside the bottle body 10 is connected with one end of another straight tube or arc-shaped tube, and the other end of the straight tube or arc-shaped tube is provided with a connecting hole 32, and the connecting hole 32 is along the radial direction perpendicular to the bottle body 10.

[0093] The T-shaped liquid inlet rod 30 can be formed by two straight tubes connected, the two straight tubes are arranged vertically, one of which is inserted toward the central axis of the bottle body 10, and the other is along the radial direction perpendicular to the bottle body 10; the T-shaped liquid inlet rod 30 can also be formed by a straight tube and an arc tube connected to each other, the straight tube is inserted toward the central axis of the bottle body 10, and the arc tube is transversely along the wall surface of the bottle body 10, and its curvature is consistent with the transverse curvature of the wall surface. The straight tube of the T-shaped liquid inlet rod 30 is provided with a liquid inlet 31 on the end surface outside the bottle body 10, and the end of the straight tube inserted toward the central axis of the bottle body 10 is located inside the bottle body 10 and is connected to the middle of another straight tube or arc tube, and both ends of the straight tube or arc tube are provided with connecting holes 32, and the two connecting holes 32 are both along the radial direction perpendicular to the bottle body 10.

[0094] In other cases, the structure of the liquid inlet rod 30 allows the liquid to enter the bottle body 10 from the liquid inlet 31 along the side wall of the bottle body 10 and flow out from the connecting hole 32. It will always flow in a spiral along the inner wall of the bottle body 10 and then smoothly enter the bottle body 10 without interfering with the probe 22. This also belongs to the same inventive concept as the present scheme.

[0095] Example 3

[0096] An immunohistochemical instrument, the difference between this embodiment and embodiment 1 is that the number of probes 22 can be four or more, which can detect more liquid level values, meet the use of automated equipment, and facilitate refined control in the operating procedure.

[0097] Example 4

[0098] Reference Figure 2 and Figure 3 , an immunohistochemistry instrument. The difference between this embodiment and embodiment 1, embodiment 2 and embodiment 3 is that the corresponding immunohistochemistry instrument can contain one, two or more solution transfer storage devices. When there is only one, the solution transfer storage device is used for reagent extraction or waste liquid collection.

[0099] At least three probes 22 are provided in the solution transfer storage device, and the three probes 22 correspond to the high position, the middle position and the low position respectively. The vent 15 can connect the bottle body 10 with the air pump, so that the bottle body 10 is in a positive pressure or negative pressure environment. When the air pump vents into the bottle body 10, the liquid reagent is discharged from the bottle body 10 through the discharge port 14 when the bottle body 10 is in a positive pressure environment; when the air is pumped out from the bottle body 10, the liquid reagent enters the bottle body 10 through the liquid inlet rod 30. Therefore, the reagent extraction and discharge of the solution transfer storage device cannot be performed at the same time.

[0100] When the solution transfer storage device is used as a waste liquid collection transfer bottle, the liquid inlet 31 of the transfer bottle is connected to the working table of the immunohistochemical instrument, and the liquid outlet is connected to the waste liquid bucket. The transfer bottle is used to recover the waste liquid after chemical reaction or cleaning on the working table, and automatically discharges the waste liquid into the waste liquid bucket when a certain amount is reached. When it is working, according to the waste liquid on the working table of the immunohistochemical instrument, it starts to extract waste liquid from the transfer bottle at any time. At this time, the liquid level in the transfer bottle rises. Generally, when it reaches the middle position, it must be reminded to change to discharge waste liquid from the transfer bottle, and it can be stopped at least when it reaches the low position. If the waste liquid is not discharged at the middle position, the waste liquid on the working table of the immunohistochemical instrument continues to accumulate in the transfer bottle, and when it reaches the high position, it must start to discharge the waste liquid. If it is not started, the immunohistochemical instrument will stop working, that is, the tissue sample staining program on the working table is terminated. Therefore, setting up three probes 22 can ensure that the immunohistochemical instrument automatically determines when to start waste liquid extraction and discharge according to the liquid level, avoiding interruption or termination of the experiment due to failure to discharge the waste liquid in time, and realizing automated continuous operation.

[0101] When the solution transfer storage device is used as a cleaning liquid transfer bottle, the liquid outlet of the transfer bottle is connected to the shower head of the immunohistochemical instrument, and the liquid inlet 31 is connected to a large reagent bottle containing cleaning liquid. The transfer bottle is used to store the cleaning liquid, so that the shower head can output the cleaning liquid at any time to perform shower operation on the slide containing tissue samples on the working table of the immunohistochemical instrument. When it is working, before the immunohistochemical instrument is started, the cleaning liquid needs to be extracted from the large reagent bottle into the transfer bottle, and to the highest liquid level, to ensure that there is sufficient cleaning liquid during the operation, so as not to delay the immunohistochemical instrument staining experiment cycle; during the staining experiment, it is necessary to detect the liquid level at all times. When the liquid level reaches the lowest level, the extraction of cleaning liquid must be started, and the discharge of cleaning liquid can be started at least when it reaches the middle level, that is, the showering work of the immunohistochemical instrument, to avoid the liquid level in the transfer bottle being lower than the lowest level, and the remaining cleaning liquid cannot meet the showering operation of an experimental batch, resulting in a batch of tissue samples that cannot be processed synchronously, poor consistency, and affect the staining quality; during the staining experiment, the showering is turned on after each extraction to the middle position, to avoid the extraction time being too long, and the lack of timely showering, which prolongs the staining time of the tissue sample and affects the staining effect. The capacity difference corresponding to the middle position and the low position here at least achieves the showering operation volume of one batch of the immunohistochemical instrument, avoiding the extraction of cleaning liquid once during the experiment, and not being able to meet the discharge of the showering operation volume of one batch.

[0102] The embodiments of this specific implementation are all preferred embodiments of the present application, and are not intended to limit the protection scope of the present application. The same components are represented by the same figure marks. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A solution transfer storage device, characterized in that: include: A bottle body (10), wherein the bottle body (10) is arranged in a cylindrical shape, and a liquid discharge port (14) is arranged at the bottom of the bottle body (10); A probe assembly (20), the probe assembly (20) being capable of being inserted into the bottle body (10) and being used to detect liquid level information in the bottle body (10); A liquid inlet rod (30), the liquid inlet rod (30) being inserted into the bottle body (10) along a direction toward the central axis of the bottle body (10); The end surface of the liquid inlet rod (30) located outside the bottle body (10) is provided with a liquid inlet (31), and the end surface located inside the bottle body (10) is blocked; the side wall of the end of the liquid inlet rod (30) located inside the bottle body (10) is provided with a communication hole (32), and the communication hole (32) allows liquid to enter the bottle body (10) from the liquid inlet (31) along the side wall of the bottle body (10).

2. A solution transfer storage device according to claim 1, characterized in that: The liquid inlet rod (30) is in a tubular structure, and the direction of the communication hole (32) is perpendicular to the radial direction of the bottle body (10).

3. A solution transfer storage device according to claim 2, characterized in that: There are two communicating holes (32), and the two communicating holes (32) are arranged horizontally opposite to each other; and / or The side wall of the bottle body (10) is provided with reinforcing ribs (13); and / or The bottle body (10) is provided with a connecting pipe (11), the connecting pipe (11) is in communication with the bottle body (10), and the connecting pipe (11) is provided with a first limiting member (111); the liquid inlet rod (30) is provided with a second limiting member (33), and the liquid inlet rod (30) is inserted into the connecting pipe (11) in a circumferentially limited manner through the cooperation between the first limiting member (111) and the second limiting member (33).

4. A solution transfer storage device according to claim 1, characterized in that: The probe assembly (20) comprises a probe seat (21) and a probe (22); the probe seat (21) is arranged at the bottle mouth of the bottle body (10); the probe (22) is fixedly connected to the probe seat (21); a protection plate (211) is arranged on the probe seat (21); the protection plate (211) is located between the probe (22) and the liquid inlet rod (30) and is used to block liquid inlet disturbance in the direction of the liquid inlet rod (30).

5. A solution transfer storage device according to claim 4, characterized in that: The protection plate (211) is an arc protection plate (211).

6. A solution transfer storage device according to claim 5, characterized in that: The probe seat (21) is provided with a third limiting member (212), and the bottle body (10) is provided with a fourth limiting member (12). The probe seat (21) is inserted into the bottle body (10) in a circumferentially limited manner through the cooperation between the third limiting member (212) and the fourth limiting member (12), and the protection plate (211) is located on the side that blocks the liquid inlet rod (30) from inletting liquid.

7. A solution transfer storage device according to claim 4, characterized in that: It also comprises a liquid level detection plate (40), the liquid level detection plate (40) being located at the top end of the probe base body (21), and the probe (22) passing through the probe base body (21) and being in electrical contact with the liquid level detection plate (40).

8. A solution transfer storage device according to claim 4, characterized in that: The probes (22) are at least three, namely a first probe (221), a second probe (222) and a third probe (223), and the depths of the first probe (221), the second probe (222) and the third probe (223) extending into the bottle body (10) increase sequentially.

9. A solution transfer storage device according to claim 7, characterized in that: A waterproof membrane structure is provided on one side of the probe seat (21) facing the inside of the bottle body (10) to form a plurality of mutually spaced grooves (213), and the probes (22) correspond to the grooves (213) one by one and are located in the corresponding grooves (213).

10. An immunohistochemical instrument, characterized in that: It comprises a solution transfer storage device as described in any one of claims 1 to 9.

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