Double-target gene methylation detection kit

Abstract

The utility model discloses a double-target gene methylation detection kit, which relates to the technical field of reagent packaging and comprises an openable shell, a reagent storage box is arranged in the shell, filler with a plurality of rows of reagent grooves is arranged in the reagent storage box, and the reagent grooves are used for placing reagent bottles. A first refrigeration medium is placed in a placing groove formed in the part, between every two adjacent rows of reagent grooves, of the filler. Through the arrangement of the reagent storage box, the filler and the first refrigeration medium, after the detection kit is taken out of the refrigeration equipment, the temperature rise speed of the first refrigeration medium is far lower than that of a detection reagent, and the first refrigeration medium can penetrate through the filler from the side face of a reagent bottle in a heat conduction mode to cool the reagent bottle; the effect of cooling and refrigerating the detection reagent can be achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of reagent packaging, in particular to a dual-target gene methylation detection kit. Background Art

[0002] The dual-target gene methylation detection kit usually contains reagents such as conversion solution, binding solution, washing solution, purification solution, elution solution, droplet generation oil, and tools such as well plates. For different manufacturers, the quantity and type of reagents will vary slightly depending on their detection technology. Generally speaking, methylation detection reagents need to be stored at low temperatures to maintain their stability and activity. Therefore, the kit needs to be stored in a refrigerated device, and the kit needs to be taken out of the refrigerated device when used.

[0003] Currently, conventional test kits only use foam as the main filling, and several reagent grooves are reserved on the foam to limit the reagent bottles. They cannot maintain a low-temperature environment for a period of time after being removed from the refrigeration equipment. During the experimental operation, the temperature of the reagent will affect its stability and activity. Utility Model Content

[0004] The utility model provides a dual-target gene methylation detection kit, which can maintain a certain refrigeration effect after the kit is taken out from the refrigeration equipment, and at least solves some of the problems existing in the above-mentioned prior art.

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0006] A dual-target gene methylation detection kit comprises an openable shell, which may be a paper box, a reagent storage box arranged in the shell, a filler arranged in the reagent storage box, which may be pearl cotton, a plurality of rows of reagent slots are provided on the filler, each row of reagent slots has a plurality of reagent slots, the reagent slots are used to place reagent bottles, a placement slot is provided on the portion of the filler located between two adjacent rows of reagent slots, a first refrigeration medium is placed in the placement slot, when the detection kit is placed in a refrigeration device, the first refrigeration medium will also gradually cool down, when the detection kit is taken out of the refrigeration device, the first refrigeration medium will heat up much slower than the detection reagent, and thus can play a role in cooling and refrigerating the detection reagent.

[0007] Preferably, a bottom groove is provided at the bottom of the filler, a second refrigeration medium is placed in the bottom groove, and the projection of the reagent groove on the horizontal plane is located within the projection range of the second refrigeration medium on the horizontal plane. The size of the second refrigeration medium is larger than the first refrigeration medium, the second refrigeration medium cools the detection reagent from the bottom, and the first refrigeration medium cools the detection reagent from the side.

[0008] Preferably, the placement tank is communicated with the bottom tank, and the first refrigeration medium is in contact with the second refrigeration medium, so that the temperatures of the first refrigeration medium and the second refrigeration medium can be more uniform.

[0009] Preferably, the first refrigeration medium and the second refrigeration medium are ice packs, or other medium with large specific heat capacity. The surface of the ice pack is a plastic film, so when the ice pack is inserted into the placement slot, the ice pack can automatically fit and deform according to the shape of the placement slot.

[0010] Preferably, a bottom pad is provided in the part of the reagent storage box located at the side of the bottom groove away from the reagent groove. The material of the bottom pad can be the same as that of the filler, and plays the role of isolating the second refrigeration medium and the reagent storage box body.

[0011] Preferably, a utensil storage tray is provided on the top of the reagent storage box, and the utensil storage tray can cover the top of the reagent storage box.

[0012] Preferably, a groove is provided on the top inner side wall of the reagent storage box, and a convex edge matching the groove is provided on the utensil storage tray.

[0013] Preferably, a drawstring is fixed to the utensil storage tray.

[0014] Preferably, the cross section of the placement groove is corrugated on one side close to the reagent groove, and the reagent groove is located between the valleys of adjacent placement grooves, so that the first refrigerated medium can wrap as many reagent bottles as possible.

[0015] Compared with the prior art, the beneficial effects of the present invention are:

[0016] 1. By setting a reagent storage box and a filler, and placing a first refrigerating medium in a placement slot opened in the portion where the filler is located between two adjacent rows of reagent slots, when the test kit is placed in the refrigerating device, the first refrigerating medium will gradually cool down. When the test kit is taken out of the refrigerating device, the heating rate of the first refrigerating medium is much lower than that of the test reagent. The first refrigerating medium can cool down the test reagent bottle through the filler in the form of heat conduction from the side of the test reagent bottle, thereby playing a role in cooling and refrigerating the test reagent.

[0017] 2. By setting the cross-section of the placement groove close to the side of the reagent groove into a wave shape, when the first refrigeration medium such as an ice bag is placed in the placement groove, the ice bag will also be wavy, and because the reagent groove is located between the troughs of adjacent placement grooves, the first refrigeration medium can wrap as many reagent bottles as possible, making the temperature of the detection reagent more uniform and achieving a better refrigeration effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a three-dimensional diagram of the overall structure of the utility model;

[0019] Figure 2 This is a schematic diagram of the coordination of the reagent storage box and the utensil storage tray of the utility model;

[0020] Figure 3 It is a structural schematic diagram of the tool storage tray of the utility model;

[0021] Figure 4 This is a schematic diagram of the structure of the reagent storage box and the filler of the utility model;

[0022] Figure 5 It is a longitudinal sectional view of the reagent storage box of the utility model;

[0023] Figure 6 It is a transverse cross-sectional view of the reagent storage box of the utility model;

[0024] Figure 7 This is a bottom perspective stereogram of the filler of the utility model.

[0025] In the figure: 1, shell, 2, reagent storage box, 3, filler, 4, reagent slot, 5, placement slot, 6, first refrigeration medium, 7, bottom slot, 8, second refrigeration medium, 9, bottom pad, 10, utensil storage tray, 11, groove, 12, convex edge, 13, trough, 14, drawstring. DETAILED DESCRIPTION

[0026] The following is a clear and complete description of the technical solutions in the implementation of the utility model in conjunction with the accompanying drawings. The described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] Example 1

[0028] like Figures 1 to 7 As shown, a dual-target gene methylation detection kit provided by Example 1 of the utility model includes an openable shell 1, on which functional information is printed or pasted. The shell 1 can be a conventional paper box, having an inner cavity for containing and a top cover that can be opened and closed. A reagent storage box 2 is placed in the shell 1, and the size of the reagent storage box 2 is slightly smaller than the shell 1. The top of the reagent storage box 2 is open, but the utensil storage tray 10 placed on the top can cover the top of the reagent storage box 2. The utensil storage box can be used to place some well plates or sample collection tools for experiments.

[0029] A filler 3 is fixed in the reagent storage box 2, and the filler 3 can be a material with buffering ability such as pearl cotton. Figure 4As shown, eight rows of reagent slots 4 are provided on the filler 3, and each row of reagent slots 4 has four reagent slots 4, and reagent bottles can be inserted into the reagent slots 4. Through the above arrangement, the reagent kit has the function of storing reagents, and in order to solve the problem of refrigerated storage of reagents, as Figures 5 to 7 As shown, in this embodiment, a placement groove 5 is provided in the portion where the filler 3 is located between two adjacent rows of reagent grooves 4. The shape of the placement groove 5 can be a long strip or other shapes. A first refrigeration medium 6 can be placed in the placement groove 5, and the first refrigeration medium 6 is preferably an ice bag. After the test kit is placed in the refrigeration device, the ice bag will gradually cool down. When the test kit is taken out of the refrigeration device, the temperature rise rate of the ice bag is much lower than that of the test reagent, so it can play a role in cooling and refrigerating the test reagent. Placing the ice bag between two adjacent rows of reagents can expand the range of influence of the ice bag on the reagent bottle in the vertical direction.

[0030] Example 2

[0031] On the basis of Example 1, Figure 6 and Figure 7 As shown, the cross section of the placement groove 5 can be set to a double-sided wavy shape, that is, the side walls of the placement groove 5 close to the reagent grooves 4 on both sides are all corrugated, and the reagent groove 4 is located between the troughs 13 of two adjacent placement grooves 5, so that the first refrigeration medium 6 can wrap as many reagent bottles as possible, thereby improving the refrigeration effect of the first refrigeration medium 6 on the reagent bottles. The surface of the ice bag is a plastic film, so when the ice bag is inserted into the placement groove 5, the ice bag can automatically fit and deform according to the shape of the placement groove 5.

[0032] Example 3

[0033] On the basis of Example 1, in order to increase the capacity of the refrigeration medium, a bottom groove 7 is further provided at the bottom of the filler 3, and a flat second refrigeration medium 8 is placed in the bottom groove 7. The second refrigeration medium 8 can be made of the same material as the first refrigeration medium 6, or it can be different, and it can be an ice bag or other medium with a large specific heat capacity. The second refrigeration medium 8 can cool down all reagent bottles from the bottom of the reagent bottles, that is, the projection of the reagent groove 4 on the horizontal plane is located within the range of the projection of the second refrigeration medium 8 on the horizontal plane. In this way, the second refrigeration medium 8 cools down the detection reagent from the bottom, while the first refrigeration medium 6 cools down the detection reagent from the side, and the side and bottom of the reagent bottle can be affected by the refrigeration medium, thereby achieving a more uniform refrigeration effect.

[0034] The placement slot 5 is connected to the bottom slot 7, so it is convenient to put an ice bag into each placement slot 5 from the direction of the bottom slot 7. Although the first refrigeration medium 6 and the second refrigeration medium 8 exist independently, they are in contact with each other, which can also enable heat conduction between the first refrigeration medium 6 and the second refrigeration medium 8, so that the temperatures of the first refrigeration medium 6 and the second refrigeration medium 8 are relatively consistent.

[0035] Since the first refrigerated medium 6 is inside the filler 3, and the filler 3 such as pearl cotton has a certain degree of heat insulation, the temperature rise of the first refrigerated medium 6 can be slowed down. In order to slow down the temperature rise of the second refrigerated medium 8, a bottom pad 9 is placed in the reagent storage box 2 at the side of the bottom groove 7 away from the reagent groove 4. The material of the bottom pad 9 can be the same as that of the filler 3, which plays the role of isolating the second refrigerated medium 8 from the box body of the reagent storage box 2 and slowing down the temperature rise rate of the second refrigerated medium 8.

[0036] Example 4

[0037] On the basis of Example 1, in order to isolate moisture in the air as much as possible and avoid condensation in the reagent storage box 2, a groove 11 is provided on the top inner side wall of the reagent storage box 2, and a convex edge 12 cooperating with the groove 11 is provided on the tool storage tray 10. When the convex edge 12 is inserted into the groove 11, the convex edge 12 and the groove 11 are interference-fitted, so that it is difficult for outside air to enter the reagent storage box 2. At the same time, a moisture-proof agent can be placed in the reagent storage box 2 to further absorb moisture. Because the convex edge 12 cooperates with the groove 11, it is difficult to take out the tool storage tray 10, so two drawstrings 14 are fixed on the tool storage tray 10 to facilitate the staff to pull the tool storage tray 10.

[0038] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit and basic features of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim involved.

[0039] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A dual-target gene methylation detection kit, comprising an openable shell (1), characterized in that: A reagent storage box (2) is arranged in the housing (1), a filler (3) is arranged in the reagent storage box (2), a plurality of rows of reagent slots (4) are provided on the filler (3), the reagent slots (4) are used to place reagent bottles, and a placement slot (5) is provided in the portion of the filler (3) located between two adjacent rows of reagent slots (4), a first refrigeration medium (6) is placed in the placement slot (5).

2. A dual-target gene methylation detection kit as claimed in claim 1, characterized in that: A bottom groove (7) is provided at the bottom of the filler (3), a second refrigeration medium (8) is placed in the bottom groove (7), and a projection of the reagent tank (4) on the horizontal plane is located within the range of the projection of the second refrigeration medium (8) on the horizontal plane.

3. A dual-target gene methylation detection kit as claimed in claim 2, characterized in that: The placement groove (5) is communicated with the bottom groove (7), and the first refrigeration medium (6) is in contact with the second refrigeration medium (8).

4. A dual-target gene methylation detection kit as claimed in claim 3, characterized in that: The first refrigeration medium (6) and the second refrigeration medium (8) are ice packs.

5. A dual-target gene methylation detection kit as claimed in claim 2, characterized in that: A bottom pad (9) is provided in the reagent storage box (2) at a portion of the bottom groove (7) on a side away from the reagent groove (4).

6. A dual-target gene methylation detection kit as claimed in claim 1, characterized in that: A utensil storage tray (10) is provided on the top of the reagent storage box (2), and the utensil storage tray (10) is capable of covering the top of the reagent storage box (2).

7. A dual-target gene methylation detection kit as claimed in claim 6, characterized in that: A groove (11) is provided on the inner side wall of the top of the reagent storage box (2), and a convex edge (12) matching with the groove (11) is provided on the tool storage tray (10).

8. A dual-target gene methylation detection kit as claimed in claim 6, characterized in that: A drawstring (14) is fixed on the utensil storage tray (10).

9. A dual-target gene methylation detection kit as claimed in claim 1, characterized in that: The cross section of the placement groove (5) is in a corrugated shape on the side close to the reagent groove (4), and the reagent groove (4) is located between the valleys (13) of adjacent placement grooves (5).

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