Cell separating and sampling device

Abstract

The utility model relates to a cell separating and sampling device, which belongs to the technical field of cell separation and comprises an outer cylinder, a stratified sampling mechanism is arranged in the outer cylinder and comprises a first sampling cavity fixedly mounted on the surface of an inner cavity of the outer cylinder, and a second sampling cavity is fixedly mounted at the top of the first sampling cavity. A third sampling cavity is fixedly mounted at the top of the second sampling cavity, and a sampling outer pipe communicated with the interior of the outer cylinder is fixedly mounted on the outer side surface of the outer cylinder. According to the cell separating and sampling device, the stratified sampling mechanism is arranged in the outer cylinder, so that a liquid sample can be independently extracted in a stratified manner during use, stratified sampling of the liquid sample can be realized, and the situation that a detection result is influenced by sample pollution caused by mixing of the liquid samples at different layers can be prevented; the problems that in the prior art, due to the fact that a sampling needle tube is inserted into layered liquid and then the layered liquid is sampled, the collection operation speed is low, and operation is inconvenient are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell separation, in particular to a cell separation sampling device. Background Art

[0002] Cell separation refers to the process of separating different types of cells from a mixed cell population. Cell separation is often used in biomedical research, clinical diagnosis, drug development and other fields to obtain pure samples of specific types of cells for more precise experiments and analysis.

[0003] Chinese patent CN215492619U discloses a cell separation sampling device, comprising an outer shell, a centrifugal shell, a driver for driving the centrifugal shell to rotate axially in the outer shell, and an outer cover covering the outer shell and sealing the centrifugal shell. A plurality of absorption tubes are axially slid in sequence in the centrifugal shell. The cell separation sampling device also includes a flexible tube connected to the absorption tube and driving the absorption tube to move axially in the centrifugal shell. The surface of the absorption tube is provided with an absorption hole connecting the flexible tube and the inside of the centrifugal shell. One end of the flexible tube away from the absorption tube is passed through the periphery of the centrifugal shell, which solves the problems of inconvenience in manual extraction and collection of existing cells after centrifugal stratification and easy local mixing of stratified cells during cell extraction, which affects the collected data. The stratified collection of cells is more convenient, the manual operation is more convenient, and the influence of the mixing of stratified cells during extraction and collection is effectively avoided.

[0004] The above patent improves the shortcomings of the prior art of inserting a sampling needle into the stratified liquid to sample the stratified liquid, which has slow collection speed and inconvenient operation. This embodiment provides another implementation scheme for the problems raised in the above patent. Utility Model Content

[0005] In view of the shortcomings of the prior art, the utility model provides a cell separation sampling device, which has the advantage of convenient collection and solves the problem that the prior art inserts a sampling needle into the stratified liquid to sample the stratified liquid, but has the problem of slow collection operation and inconvenient operation.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell separation sampling device, comprising an outer cylinder, wherein a layered sampling mechanism is provided in the outer cylinder;

[0007] The stratified sampling mechanism includes a first sampling cavity fixedly mounted on the inner cavity surface of the outer cylinder, a second sampling cavity fixedly mounted on the top of the first sampling cavity, a third sampling cavity fixedly mounted on the top of the second sampling cavity, a sampling outer tube connected to the inside of the outer cylinder fixedly mounted on the outer surface of the outer cylinder, a rubber sealing plate fixedly mounted on the inner surface of the sampling outer tube, micro pumps fixedly mounted on the surfaces of the first sampling cavity, the second sampling cavity and the third sampling cavity, a micro electric telescopic rod fixedly mounted on the surface of the micro pump, a connecting plate fixedly mounted on one end of the micro electric telescopic rod, a sampling inner tube fixedly mounted on the surface of the connecting plate, a telescopic hose fixedly mounted between the sampling inner tube and the liquid inlet end of the micro pump, and a liquid discharge valve penetrating to the outer surface of the outer cylinder fixedly mounted on the surfaces of the first sampling cavity, the second sampling cavity and the third sampling cavity.

[0008] Furthermore, a connecting rod is fixedly installed on the upper surface of the outer cylinder, a threaded rod is fixedly installed on the top of the connecting rod, and a support plate is threadedly connected to the surface of the threaded rod. The outer cylinder is suspended in the liquid collection container by the cooperation of the threaded rod and the support plate, and the position of the support plate can be adjusted at the same time.

[0009] Furthermore, two stoppers are fixedly mounted on the surface of the threaded rod, and the support plate is located between the two stoppers.

[0010] The beneficial effect of adopting the above further solution is that it helps to prevent the support plate from detaching from the threaded rod.

[0011] Furthermore, a cross cut is provided on the surface of the rubber sealing plate.

[0012] The beneficial effect of adopting the above further solution is that a cross cut is provided on the rubber sealing plate, so that the inner sampling tube can pass through the rubber sealing plate to take liquid.

[0013] Furthermore, a threaded hole is provided on the surface of the support plate, and the outer surface of the threaded rod is threadedly connected to the inner surface of the threaded hole.

[0014] The beneficial effect of adopting the above further solution is that it is easy to adjust the position of the support plate on the threaded rod.

[0015] Furthermore, the first sampling cavity, the second sampling cavity and the third sampling cavity are not communicated with each other.

[0016] The beneficial effect of adopting the above further solution is that it is convenient to store different liquid samples separately.

[0017] Furthermore, the inner diameter of the sampling outer tube is larger than the outer diameter of the sampling inner tube, and the number of the micro electric telescopic rods on the connecting plate is two.

[0018] The beneficial effect of adopting the above further solution is that it is easy to insert the sampling inner tube into the sampling outer tube.

[0019] Compared with the prior art, the technical solution of this application has the following beneficial effects:

[0020] The cell separation sampling device is provided with a stratified sampling mechanism in an outer cylinder. When in use, liquid samples can be extracted separately in layers, thereby realizing stratified sampling of liquid samples. This can prevent the mixing of liquid samples in different layers, which would cause sample contamination and affect the test results. This solves the problem in the prior art of inserting a sampling needle into the stratified liquid and then sampling the stratified liquid, which results in slow collection operation and inconvenience in operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the interior of the outer cylinder of the utility model structure;

[0022] Figure 2 This is a schematic diagram of the appearance of the outer cylinder of the utility model structure;

[0023] Figure 3 This is a schematic diagram of the structural support plate of the utility model;

[0024] Figure 4 This is a schematic diagram of the structural rubber sealing plate of the utility model;

[0025] Figure 5 The utility model structure Figure 1 A is an enlarged schematic diagram.

[0026] In the figure: 1, outer cylinder; 2, first sampling chamber; 3, second sampling chamber; 4, third sampling chamber; 5, sampling outer tube; 6, rubber sealing plate; 7, micro pump; 8, micro electric telescopic rod; 9, connecting plate; 10, sampling inner tube; 11, telescopic hose; 12, drain valve; 13, connecting rod; 14, threaded rod; 15, support plate; 16, block; 17, cross cut; 18, threaded hole. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0028] See also Figure 1-5A cell separation sampling device in this embodiment includes an outer cylinder 1, and a stratified sampling mechanism is arranged in the outer cylinder 1. The stratified sampling mechanism includes a first sampling cavity 2 fixedly mounted on the inner cavity surface of the outer cylinder 1, a second sampling cavity 3 fixedly mounted on the top of the first sampling cavity 2, and a third sampling cavity 4 fixedly mounted on the top of the second sampling cavity 3. The first sampling cavity 2, the second sampling cavity 3 and the third sampling cavity 4 are not connected to each other, so as to facilitate the separate storage of different liquid samples. A sampling outer tube 5 connected to the inner part of the outer cylinder 1 is fixedly mounted on the outer surface of the outer cylinder 1, and a rubber sealing plate 6 is fixedly mounted on the inner surface of the sampling outer tube 5. The first sampling cavity 2, the second sampling cavity 3 and the third sampling cavity 4 are connected to each other. A micro pump 7 is fixedly mounted on the surface of the three sampling chambers 4, a micro electric telescopic rod 8 is fixedly mounted on the surface of the micro pump 7, a connecting plate 9 is fixedly mounted on one end of the micro electric telescopic rod 8, a sampling inner tube 10 inserted into the sampling outer tube 5 is fixedly mounted on the surface of the connecting plate 9, a cross cut 17 is provided on the surface of the rubber sealing plate 6, and the cross cut 17 is provided on the rubber sealing plate 6 to facilitate the sampling inner tube 10 to pass through the rubber sealing plate 6 to take liquid, a telescopic hose 11 is fixedly mounted between the sampling inner tube 10 and the liquid inlet end of the micro pump 7, and a drain valve 12 that penetrates the outer surface of the outer tube 1 is fixedly mounted on the surfaces of the first sampling chamber 2, the second sampling chamber 3 and the third sampling chamber 4.

[0029] A connecting rod 13 is fixedly installed on the upper surface of the outer cylinder 1, a threaded rod 14 is fixedly installed on the top of the connecting rod 13, and a support plate 15 is threadedly connected to the surface of the threaded rod 14. The outer cylinder 1 is suspended in the liquid collection container by the cooperation of the threaded rod 14 and the support plate 15, and the position of the support plate 15 can be adjusted at the same time. Two stoppers 16 are fixedly installed on the surface of the threaded rod 14, and the support plate 15 is located between the two stoppers 16. It is beneficial to prevent the support plate 15 from detaching from the threaded rod 14. A threaded hole 18 is opened on the surface of the support plate 15, and the outer surface of the threaded rod 14 is threadedly connected to the inner surface of the threaded hole 18, so as to facilitate the adjustment of the position of the support plate 15 on the threaded rod 14.

[0030] The inner diameter of the sampling outer tube 5 is larger than the outer diameter of the sampling inner tube 10 . There are two miniature electric telescopic rods 8 on the connecting plate 9 , which facilitates inserting the sampling inner tube 10 into the sampling outer tube 5 .

[0031] It should be noted that when the cross cut 17 on the surface of the rubber sealing plate 6 is in a closed state, liquid can be prevented from flowing into the sampling outer tube 5. When the sampling inner tube 10 passes through the rubber sealing plate 6 through the cross cut 17, the gap between the rubber sealing plate 6 and the outer surface of the sampling inner tube 10 is small.

[0032] It should be noted that the rubber sealing plate 6 in this embodiment is made of a highly elastic rubber material, which has very good elasticity and can produce corresponding deformation when squeezed by an object, and can quickly return to its original shape after losing resistance and is not easy to deform.

[0033] The working principle of the above embodiment is:

[0034] When in use, the support plate 15 is rotated to adjust the position of the support plate 15 on the threaded rod 14 so that the outer tube 1 can extend into the container. The support plate 15 is placed at the container mouth so that the outer tube 1 is suspended inside the container, so that the three sampling outer tubes 5 correspond to liquid samples at different levels respectively. The micro-electric telescopic rod 8 is started, and the connecting plate 9 and the sampling inner tube 10 are driven by the micro-electric telescopic rod 8 to move, so that the sampling inner tube 10 passes through the rubber sealing plate 6 through the cross cut 17, so that the sampling inner tube 10 contacts the liquid sample. The micro-pump 7 is started, and the micro-pump 7 draws the liquid sample into the corresponding sampling cavity through the sampling inner tube 10 and the telescopic hose 11, so that the sampling is completed. The sample in the sampling cavity can be discharged by opening the drain valve 12. It is convenient and quick to use.

[0035] The control method of the present invention is controlled by a controller. The control circuit of the controller can be realized by simple programming by technicians in this field. The provision of power is also common knowledge in this field. The present invention is mainly used to protect mechanical devices, so the present invention will no longer explain the control method and circuit connection in detail.

[0036] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0037] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell separation sampling device, comprising an outer cylinder (1), characterized in that: The outer cylinder (1) is provided with a layered sampling mechanism; The stratified sampling mechanism comprises a first sampling cavity (2) fixedly mounted on the inner cavity surface of an outer cylinder (1); a second sampling cavity (3) is fixedly mounted on the top of the first sampling cavity (2); a third sampling cavity (4) is fixedly mounted on the top of the second sampling cavity (3); a sampling outer tube (5) connected to the interior of the outer cylinder (1) is fixedly mounted on the outer surface of the outer cylinder (1); a rubber sealing plate (6) is fixedly mounted on the inner surface of the sampling outer tube (5); micro-sampling devices (14) are fixedly mounted on the surfaces of the first sampling cavity (2), the second sampling cavity (3) and the third sampling cavity (4). A micro pump (7) is provided, wherein a micro electric telescopic rod (8) is fixedly mounted on the surface of the micro pump (7), a connecting plate (9) is fixedly mounted on one end of the micro electric telescopic rod (8), a sampling inner tube (10) plugged into a sampling outer tube (5) is fixedly mounted on the surface of the connecting plate (9), a telescopic hose (11) is fixedly mounted between the sampling inner tube (10) and the liquid inlet end of the micro pump (7), and a liquid discharge valve (12) penetrating to the outer surface of the outer tube (1) is fixedly mounted on the surfaces of the first sampling cavity (2), the second sampling cavity (3) and the third sampling cavity (4).

2. A cell separation sampling device according to claim 1, characterized in that: A connecting rod (13) is fixedly mounted on the upper surface of the outer cylinder (1), a threaded rod (14) is fixedly mounted on the top of the connecting rod (13), and a support plate (15) is threadedly connected to the surface of the threaded rod (14).

3. A cell separation sampling device according to claim 2, characterized in that: Two stoppers (16) are fixedly mounted on the surface of the threaded rod (14), and the support plate (15) is located between the two stoppers (16).

4. A cell separation sampling device according to claim 1, characterized in that: The surface of the rubber sealing plate (6) is provided with a cross cutout (17).

5. A cell separation sampling device according to claim 2, characterized in that: A threaded hole (18) is provided on the surface of the support plate (15), and the outer surface of the threaded rod (14) is threadedly connected to the inner surface of the threaded hole (18).

6. A cell separation sampling device according to claim 1, characterized in that: The first sampling chamber (2), the second sampling chamber (3) and the third sampling chamber (4) are not interconnected.

7. A cell separation sampling device according to claim 1, characterized in that: The inner diameter of the sampling outer tube (5) is greater than the outer diameter of the sampling inner tube (10), and the number of the micro electric telescopic rods (8) on the connecting plate (9) is two.

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