Sterile liquid adding and sampling device
By designing a sterile liquid addition sampling device, the isolation cavity is formed using the piston ring and limit structure, the problems of complex and contamination risks of sterile sampling and liquid addition operations in the prior art are solved, and efficient and simple sterile operation and high-precision cell sampling are achieved.
Patent Information
- Application Number
- CN202420947853.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-02
- Publication Date
- 2025-03-18
- Estimated Expiration
- 2034-05-02
AI Technical Summary
The existing sterile sampling or liquid addition operations rely on the sterile environment, are complex in operation and have the risk of contamination. Especially during cell culture, existing equipment is difficult to effectively ensure sterility and simplicity of operation.
A sterile liquid-adding sampling device is designed, including a sampling cylinder, a piston rod and a plurality of piston rings. Through the sealing and limiting structure of the piston ring, multiple isolation chambers are formed to ensure the sterility and sealing of the sampling and liquid-adding process.
This device realizes efficient and simplified sterile sampling and addition liquid, significantly reduces the probability of cells or reagents being contaminated, avoids reagent waste, and supports quantitative, sterile, disposable high-precision cell sampling.
Smart Images
Figure CN222631432U_ABST
Abstract
Description
Technical Field
[0001] The utility model particularly relates to an aseptic liquid adding sampling device, belonging to the technical field of drug sampling and aseptic liquid adding. Background Art
[0002] In the production process of sterile drugs, aseptic sampling or liquid addition operations are often required. In this process, aseptic operation is required. Generally, the equipment is moved into a sterile environment, and the items that are at risk of contact with the reagents are disinfected or sterilized, and then the sampling is completed, or a more complex isolation and sterilization device is used for sampling or liquid addition. Relatively speaking, the operation is cumbersome and complicated, especially for cell culture operations. In the production process of cell-based drugs, cell sampling needs to be performed regularly to monitor indicators such as cell activity, loss rate and density. At the same time, the detection of the cell growth process also requires liquid addition operations to adjust its growth environment. Therefore, the device for aseptic liquid addition or sampling operations is particularly important.
[0003] Existing sampling or liquid addition operations are particularly dependent on a sterile environment and require aseptic operation or sterilization. The preparation time is long and the operation is complicated. The commonly used sampling syringes on the market are generally disposable sterilized syringes. After opening, the inner wall surface of the syringe is directly exposed to the air. After pulling open the piston rod in the syringe, the internal part originally exposed to the air enters the front end of the piston rod and directly contacts the reagent liquid, posing a risk of contamination. Utility Model Content
[0004] The main purpose of the utility model is to provide a sterile liquid adding sampling device, so as to overcome the shortcomings of the prior art.
[0005] In order to achieve the above-mentioned utility model purpose, the technical solution adopted by the utility model includes:
[0006] The utility model provides an aseptic liquid adding sampling device, comprising: a sampling cylinder, a piston rod and N piston rings, wherein the front end of the sampling cylinder is provided with a sampling port, and the rear end is provided with an opening for the piston rod to extend.
[0007] N piston rings are arranged on the piston rod at intervals along the axial direction of the piston rod and are located in the sampling barrel. The piston ring can reciprocate along the axial direction of the sampling barrel in the sampling barrel together with the piston rod. The piston ring is always in sealing contact with the inner wall of the sampling barrel, wherein a sampling chamber is enclosed between the first piston ring close to the sampling port and the front end of the sampling barrel, and a sealed isolation chamber is formed between any two adjacent piston rings among the N piston rings. The sampling chamber and the isolation chamber are sealed and isolated by the piston ring, and N≥2;
[0008] Furthermore, a limiting structure is provided on the inner wall of the sampling cylinder, and the movable area of the piston ring is limited between the limiting structure and the front end of the sampling cylinder.
[0009] Furthermore, the maximum distance between the Nth piston ring and the limiting structure is less than or equal to the distance between the first piston ring and the second piston ring.
[0010] Furthermore, when the piston rod moves along the axial direction of the sampling cylinder toward the front end of the sampling cylinder and reaches the limit position, after the aseptic liquid adding sampling device is sterilized, the area in the sampling cylinder corresponding to the first piston ring and the sampling port of the sampling cylinder forms a liquid adding area, the area corresponding to the first piston ring and the second piston ring forms a sterile area, and the area corresponding to the Nth piston ring and the open mouth of the sampling cylinder forms a sterile area. The limiting structure is arranged in the sterile area. Under the limitation of the limiting structure, the first piston ring is restricted to move in the sterile area, and the Nth piston ring is restricted to move in the sterile area.
[0011] Furthermore, a sterile transition zone is formed in the sampling cylinder corresponding to the area between the second piston ring and the N-1th piston ring. Under the restriction of the limiting structure, the second piston ring to the N-1th piston ring are movable in the sterile transition zone.
[0012] Furthermore, the internal space of the sampling tube is a cylindrical structure with a uniform tube diameter.
[0013] Furthermore, the first piston ring is fixedly arranged at the end of the piston rod, and the N piston rings are coaxially arranged with the piston rod.
[0014] Furthermore, the piston rod and the sampling cylinder are coaxially arranged.
[0015] Furthermore, at least the surface portion of the piston ring close to its own circumferential side surface is flexible, and the portion of the piston ring in contact with the inner wall of the sampling tube is in an elastically compressed state.
[0016] Furthermore, the limiting structure includes annular protrusions continuously arranged along the circumference of the sampling barrel, or the limiting structure includes a plurality of protrusions arranged at intervals along the circumference of the sampling barrel.
[0017] Furthermore, the limiting structure and the sampling tube are integrally arranged.
[0018] Furthermore, the limiting structure is formed by a portion of the sampling tube protruding inwardly along its own radial direction.
[0019] Furthermore, the sampling tube is transparent, and is also provided with scale marks indicating the volume.
[0020] Furthermore, a sampling needle is provided at the front end of the sampling cylinder, and the sampling needle is connected to the sampling port.
[0021] Compared with the prior art, the advantages of the utility model include:
[0022] The utility model provides an aseptic liquid addition sampling device, which has simple sampling operation, can effectively ensure aseptic sampling, and greatly reduces the probability of cell or reagent contamination;
[0023] The utility model provides an aseptic liquid adding sampling device, which avoids reagent waste and has the characteristics of controllable sampling;
[0024] The utility model provides a sterile liquid adding sampling device, which can realize quantitative, sterile, disposable and high-precision cell sampling;
[0025] The utility model provides an aseptic liquid adding sampling device which can be used in conjunction with a pipeline valve and can achieve continuous liquid adding through multiple pulling and drawing actions. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0027] Figure 1 It is a structural schematic diagram of a sterile liquid adding sampling device provided in Example 1 of the utility model;
[0028] Figure 2 This is a schematic structural diagram of a sterile liquid adding sampling device provided in Example 1 of the utility model, in which the piston rod is in a fully pushed-in and fully pulled-out state;
[0029] Figure 3 It is a structural schematic diagram of a sterile liquid adding sampling device provided in Example 2 of the utility model;
[0030] Figure 4 It is a structural schematic diagram of a sterile liquid adding sampling device provided in Example 2 of the utility model, in which the piston rod is in a fully pushed in and fully pulled out state. DETAILED DESCRIPTION
[0031] In view of the deficiencies in the prior art, the inventor of this case has proposed the technical solution of the utility model after long-term research and extensive practice. The following will further explain the technical solution, its implementation process and principle, etc. in conjunction with the accompanying drawings and specific implementation cases.
[0032] Example 1
[0033] See also Figure 1 A sterile liquid adding sampling device includes a sampling barrel 100, a piston rod 200, and a first piston ring 310 and a second piston ring 320. The first piston ring 310 and the second piston ring 320 are fixed on the piston rod 200 at intervals along the axial direction of the piston rod 200. The first piston ring 310 and the second piston ring 320 are arranged in the sampling barrel 100 and can reciprocate along the axial direction of the sampling barrel 100 together with the piston rod 200 in the sampling barrel 100 under the driving of external force, thereby realizing the suction and transfer of liquid reagents.
[0034] Specifically, the front end of the sampling barrel 100 is provided with a sampling port, and the rear end is provided with an opening 110 for the piston rod 200 to extend out, and the front end and the rear end of the sampling barrel 100 are arranged opposite to each other along the axial direction of the sampling barrel 100. More specifically, the interior of the sampling barrel 100 is a cylindrical structure with a uniform barrel diameter, and the inner wall of the sampling barrel 100 is preferably a continuous and smooth curved surface structure, and the first piston ring 310 and the second piston ring 320 are adaptively and tightly fitted with the inner wall of the sampling barrel 100, while ensuring that the first piston ring 310, the second piston ring 320 and the sampling barrel 100 maintain a good sealing state, and will not affect the reciprocating movement of the first piston ring 310 and the second piston ring 320 in the sampling barrel 100.
[0035] Specifically, the sampling tube 100 is preferably transparent, and a scale mark (i.e., a graduation) indicating the volume is also provided on the sampling tube 100. The combination of the transparent sampling tube 100 and the scale mark is conducive to intuitively observing the amount of liquid added or sampled, and facilitates precise control of the amount of liquid added / sampled, so that the liquid addition and aspiration process is controllable; preferably, the scale mark is preferably provided on the outer surface of the sampling tube 100.
[0036] Specifically, a sampling needle 500 is further provided at the front end of the sampling cylinder 100 , and the sampling needle 500 is communicated with the sampling port.
[0037] Specifically, the piston rod 200 is coaxially arranged with the sampling tube 100, and the first piston ring 310, the second piston ring 320 and the piston rod 200 are also coaxially arranged. More specifically, the first piston ring 310 is arranged at the end of the piston rod 200 close to the front end of the sampling tube 100, and the second piston ring 320 is arranged on the middle part between the two ends of the piston rod 200.
[0038] Specifically, the first piston ring 310 and the second piston ring 320 are interference fit with the inner wall of the sampling cylinder 100. In addition, during the reciprocating movement of the first piston ring 310 and the second piston ring 320 in the sampling cylinder 100, the first piston ring 310 and the second piston ring 320 are always in sealing contact with the inner wall of the sampling cylinder 100. Among them, a sampling chamber is formed between the first piston ring 310 near the sampling port and the front end of the sampling barrel 100, and the sampling chamber is connected to the sampling port. The sampling chamber is used to accommodate the liquid reagent sucked by the sterile liquid adding sampling device; and a closed isolation chamber is also formed between the first piston ring 310 and the second piston ring 320. The isolation chamber is located between the sampling chamber and the tail end of the sampling barrel 100. The isolation chamber can not only prevent the liquid reagent inside the sampling chamber from leaking from the tail end of the sampling barrel 100, but also prevent the external air and other pollution from entering the sampling chamber from the tail end of the sampling barrel 100 to contaminate the liquid reagent.
[0039] Specifically, the structures and sizes of the first piston ring 310 and the second piston ring 320 are the same. The surface portions of the first piston ring 310 and the second piston ring 320 close to their circumferential sides (the surfaces of the first piston ring 310 and the second piston ring 320 in contact with the inner wall of the sampling cylinder 100) are flexible, and the surface portions of the first piston ring 310 and the second piston ring 320 in contact with the inner wall of the sampling cylinder 100 are in an elastically compressed state. Based on the design of the surface portion being a flexible structure, When the first piston ring 310 and the second piston ring 320 contact the inner wall of the sampling barrel 100, the surface parts of the first piston ring 310 and the second piston ring 320 are compressed and deformed in a recoverable manner, so that the surface parts of the first piston ring 310 and the second piston ring 320 are tightly attached to the inner wall of the sampling barrel 100, thereby ensuring that the first piston ring 310, the second piston ring 320 and the inner wall of the sampling barrel 100 can move relative to each other and achieve good sealing. Exemplarily, the first piston ring 310 and the second piston ring 320 can be soft rubber rings, etc. The structure of multiple piston rings can keep the piston rod and the sampling barrel in good coaxiality, especially when used on automatic equipment, which can improve the accuracy of adding liquid or sampling.
[0040] Specifically, a limiting structure 400 is further provided in the open area 110 near the rear end of the sampling barrel 100 in the sampling barrel 100. The limiting structure 400 is located on the activity trajectory of the second piston ring 320 and is used to limit the maximum travel distance of the second piston ring 320 / piston rod 200, prevent the second piston ring 320 from escaping from the sampling barrel 100, and prevent the piston rod 200 from escaping from the sampling barrel 100. The activity area of the first piston ring 310 and the second piston ring 320 is limited between the limiting structure 400 and the front end of the sampling barrel 100.
[0041] More specifically, the limiting structure 400 includes annular protrusions continuously arranged along the circumference of the sampling barrel 100, or the limiting structure 400 includes a plurality of protrusions arranged at intervals along the circumference of the sampling barrel 100. More specifically, the limiting structure 400 and the sampling barrel 100 are integrally arranged, and as a preferred embodiment, the limiting structure 400 is formed by a part of the sampling barrel 100 protruding inwardly along its own radial direction. As a specific implementation scheme, the limiting structure 400 has a first side surface and a second side surface which are arranged back to back along the axial direction of the sampling barrel 100, and the first side surface and the second side surface are smoothly connected, wherein the first side surface and the second side surface can both be inclined planes, and the angle between the first side surface / the second side surface and the axis of the sampling barrel 100 is an acute angle, or the first side surface and the second side surface are both arc surfaces, and the angle between the tangent of the first side surface / the second side surface and the axis of the sampling barrel 100 is an acute angle. The following scheme can also be adopted: one of the first side surface and the second side surface is a plane, and the other is an arc surface; by inclining the first side surface / the second side surface at a small angle, on the one hand, the limiting effect on the rubber plug ring can be guaranteed, and on the other hand, the piston rod 200 and the piston ring can be disengaged from the sampling barrel 100 as a whole by applying external force to the piston rod 200, or assembled into the sampling barrel 100 from the outside.
[0042] Specifically, along the axial direction of the sampling tube 100, the maximum distance between the second piston ring 320 and the limiting structure 400 is less than or equal to the distance between the first piston ring 310 and the second piston ring 320, that is, the use stroke of the piston rod 200 / first piston ring 310 / second piston ring 320 is less than or equal to the distance between the first piston ring 310 and the second piston ring 320. More specifically, when the sampling cylinder 100 and the piston rod 200 are assembled together, the piston rod 200 moves along the axial direction of the sampling cylinder 100 toward the front end of the sampling cylinder 100 and reaches the limit position. After the aseptic liquid adding sampling device is sterilized, the first piston ring 310 and the second piston ring 320 isolate the internal space of the sampling cylinder 100 to form three areas: the area between the first piston ring 310 and the sampling port of the sampling cylinder 100 forms a liquid adding area 101, the area between the first piston ring 310 and the second piston ring 320 forms a sterile area 102, and the area between the second piston ring 320 and the open port 110 of the sampling cylinder 100 forms a bacterial area 103. The limiting structure is arranged in the bacterial area 103, and the liquid adding area 101 is connected to the liquid adding pipeline or the sampling pipeline from the sampling port.
[0043] For details, please refer to Figure 2The first piston ring 310 and the second piston ring 320 always maintain a sealed fit with the sampling tube 100, and the area between the first piston ring 310 and the second piston ring 320 is sealed and isolated from other areas. After the aseptic liquid adding sampling device is sterilized, sterile air is formed in the area between the first piston ring 310 and the second piston ring 320, thereby forming a sterile area 102. It should be noted that, under the restriction of the limiting structure 400, the first piston ring 310 is restricted to move in the sterile area 102, and the second piston ring 320 is restricted to move in the sterile area 103.
[0044] Specifically, when sampling is performed with a sterile liquid adding sampling device after sterilization, when the piston rod 200 is pulled axially backward (i.e., in the direction away from the front end of the sampling barrel 100) along the axis of the sampling barrel 100, the first piston ring 310 and the second piston ring 320 move in the direction away from the front end of the sampling barrel 100, and the volume of the sampling chamber at the front end of the first piston ring 310 and the sampling barrel 100 gradually increases, thereby completing the liquid aspiration or sampling work. The limiting structure 400 arranged inside the sampling barrel 100 limits the maximum stroke of the piston rod 200, ensuring that the stroke of the piston rod 200 is less than the distance between the first piston ring 310 and the second piston ring 320, thereby ensuring that the first piston ring 310 can only move within the sterile area 102 of the sampling barrel 100 at all times, thereby avoiding the problem of cross-zone contamination caused by the reagent in the sampling chamber entering the bacterial area 103 of the sampling barrel 100.
[0045] Specifically, during the process of pushing and pulling the piston rod 200, the external environment of the sampling chamber in the sampling barrel 100 is always isolated by the sealed sterile air between the first piston ring 310 and the second piston ring 320. If external bacteria want to enter the sampling chamber, they must first pass through the seal of the second piston ring 320, then diffuse into the sterile air between the two piston rings, and then adhere to the inner wall of the sterile area 102 of the sampling barrel 100, and then pass through the seal of the first piston ring 310 to enter the sampling chamber. This makes it extremely unlikely that bacteria or contaminants will enter the sampling chamber.
[0046] Example 2
[0047] See also Figure 3 and Figure 4In the present embodiment, a sterile liquid adding sampling device has a structure that is basically the same as that in the embodiment 1, except that: a third piston ring 330 is further provided on the piston rod 200 of the present embodiment, and the third piston ring 330 is located between the first piston ring 310 and the second piston ring 320, and the maximum distance between the second piston ring 320 and the limiting structure 400 is less than or equal to the distance between the first piston ring 310 and the third piston ring 330. After the sterile liquid adding sampling device is sterilized, sterile air is formed in the area corresponding to the area between the first piston ring 310 and the third piston ring 330, thereby forming a sterile area 102, and a sterile transition area 104 is formed between the third piston ring 330 and the second piston ring 320. It should be noted that, under the restriction of the limiting structure 400, the first piston ring 310 is restricted to move in the sterile area 102, and the second piston ring 320 is restricted to move in the sterile area 103.
[0048] It should be noted that after adding the third piston ring 330, external bacteria or contaminants need to pass through more piston rings and the closed chambers between the first piston ring 310 and the third piston ring 330, and between the second piston ring 320 and the third piston ring 330 multiple times to reach the sampling chamber from the opening 110 of the sampling tube 100, thereby further reducing the possibility of external bacteria or contaminants entering the sampling chamber and contaminating the reagent.
[0049] It should be understood that the above embodiments are only for illustrating the technical concept and features of the utility model, and their purpose is to enable people familiar with the technology to understand the content of the utility model and implement it accordingly, and they cannot be used to limit the protection scope of the utility model. Any equivalent changes or modifications made according to the spirit of the utility model should be included in the protection scope of the utility model.
Claims
1. A sterile liquid adding sampling device, characterized in that: include: A sampling cylinder, a piston rod and N piston rings, wherein the front end of the sampling cylinder is provided with a sampling port, and the rear end is provided with an opening for the piston rod to extend. N piston rings are arranged on the piston rod at intervals along the axial direction of the piston rod and are located in the sampling barrel. The piston ring can reciprocate along the axial direction of the sampling barrel in the sampling barrel together with the piston rod. The piston ring is always in sealing contact with the inner wall of the sampling barrel, wherein a sampling chamber is enclosed between the first piston ring close to the sampling port and the front end of the sampling barrel, and a sealed isolation chamber is formed between any two adjacent piston rings among the N piston rings. The sampling chamber and the isolation chamber are sealed and isolated by the piston ring, and N≥2; Furthermore, a limiting structure is provided on the inner wall of the sampling cylinder, and the movable area of the piston ring is limited between the limiting structure and the front end of the sampling cylinder.
2. The aseptic liquid adding sampling device according to claim 1, characterized in that: The maximum distance between the Nth piston ring and the limiting structure is less than or equal to the distance between the first piston ring and the second piston ring.
3. The aseptic liquid adding sampling device according to claim 1 or 2, characterized in that: When the piston rod moves along the axial direction of the sampling cylinder toward the front end of the sampling cylinder and reaches the limit position, after the aseptic liquid adding sampling device is sterilized, a liquid adding zone is formed in the sampling cylinder corresponding to the area between the first piston ring and the sampling port of the sampling cylinder, a sterile zone is formed in the area between the first piston ring and the second piston ring, and a sterile zone is formed in the area between the Nth piston ring and the open mouth of the sampling cylinder. The limiting structure is arranged in the sterile zone. Under the restriction of the limiting structure, the first piston ring is restricted to move in the sterile zone, and the Nth piston ring is restricted to move in the sterile zone.
4. The aseptic liquid adding sampling device according to claim 3, characterized in that: An aseptic transition zone is formed in the sampling cylinder in the area corresponding to the second piston ring and the N-1th piston ring. Under the restriction of the limiting structure, the second piston ring to the N-1th piston ring are movable in the aseptic transition zone.
5. The aseptic liquid adding sampling device according to claim 1, characterized in that: The inner space of the sampling tube is a cylindrical structure with uniform tube diameter.
6. The aseptic liquid adding sampling device according to claim 1, characterized in that: The first piston ring is fixedly arranged at the end of the piston rod, and the N piston rings are coaxially arranged with the piston rod.
7. The aseptic liquid adding sampling device according to claim 6, characterized in that: The piston rod and the sampling cylinder are coaxially arranged.
8. The aseptic liquid adding sampling device according to claim 1 or 6, characterized in that: At least the surface portion of the piston ring close to its circumferential side surface is flexible, and the portion of the piston ring in contact with the inner wall of the sampling tube is in an elastically compressed state.
9. The aseptic liquid adding sampling device according to claim 1, characterized in that: The limiting structure includes an annular protrusion continuously arranged along the circumference of the sampling barrel, or the limiting structure includes a plurality of protrusions arranged at intervals along the circumference of the sampling barrel.
10. The aseptic liquid adding sampling device according to claim 9, characterized in that: The limiting structure and the sampling tube are integrally arranged.
11. The aseptic liquid adding sampling device according to claim 10, characterized in that: The limiting structure is formed by a part of the sampling tube protruding inwardly along its own radial direction.
12. The aseptic liquid adding sampling device according to claim 1, characterized in that: The sampling tube is transparent and is also provided with scale marks indicating the volume.
13. The aseptic liquid adding sampling device according to claim 1, characterized in that: A sampling needle is also provided at the front end of the sampling cylinder, and the sampling needle is communicated with the sampling port.