Tissue culture sterilization treatment device for explant of arbor plant

Abstract

The utility model relates to a sterilization treatment device for explant tissue culture of arbor plants, which relates to the technical field of plant tissue culture and comprises a box body, a soaking sterilization tank filled with disinfectant is arranged in the middle of the top surface of the box body, hangers are fixedly arranged on the top surface of the box body on two sides of the soaking sterilization tank, and a height-adjustable filter plate is hung between the two hangers; a plurality of placing holes which are uniformly distributed in a dot matrix shape are formed in the filter plate, and inserting ring plates for inserting explants of arbor plants are rotationally arranged in the placing holes; the filter plate is also provided with a driving mechanism for driving the plurality of inserting ring plates to rotate at the same time; a flushing assembly for flushing the explants with sterile water and a drying assembly for drying the explants with hot air are respectively arranged at two ends of the top surface of the box body in a sliding manner. The sterilization treatment device can simultaneously sterilize a plurality of North America arborvitae explants, and the explants are convenient and fast to take and place, so that the experiment efficiency is greatly improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of plant tissue culture, in particular to a sterilization treatment device for tissue culture of tree plant explants. Background Art

[0002] The North American cypress is a large tree of the genus Thuja of the Cupressaceae family. It is native to North America. It likes light, has a certain degree of shade tolerance, moderate cold tolerance, is not very demanding on soil, and prefers a humid climate and slightly acidic soil. Sterilization of North American cypress tissue culture is an important step to ensure that microbial contamination is avoided during plant tissue culture and to maintain the purity of the culture.

[0003] During the tissue culture of North American cypress, its explant is generally processed by disinfectant, and conventional disinfectant includes sodium hypochlorite, hydrogen peroxide, bleaching powder and low-concentration mercuric chloride etc. The selected disinfectant solution is poured into the culture bottle, and the culture bottle is gently stirred so that each part of the material is fully contacted with the disinfectant solution, and bubbles are driven out to make disinfection more thorough. However, the existing explant tissue culture sterilization treatment device is difficult to sterilize multiple North American cypress explants simultaneously, and the contact of the explant with the disinfectant is not thorough enough during sterilization, as in patent CN216931308U, the explant is covered by a mesh cover, which inevitably affects the contact with disinfectant, sterile water etc., and the sterilization effect has some adverse effects, and the explant is also relatively inconvenient to take and put, and the liquid needs to be drained or the contact liquid needs to be contacted to open the mesh cover.

[0004] To this end, we provide a device for sterilizing and treating tree plant explant tissue culture to solve the above-mentioned problems. Summary of the invention

[0005] The utility model aims to provide a device for sterilizing and treating tree plant explant tissue culture in view of the problems of the background technology.

[0006] The utility model achieves the above-mentioned purpose through the following technical solutions:

[0007] A device for tissue culture sterilization of tree plant explants comprises a box body, wherein a soaking and sterilization tank filled with disinfectant is provided in the middle of the top surface of the box body, and hangers are fixedly provided on the top surfaces of the box body on both sides of the soaking and sterilization tank, and a filter plate with adjustable height is hung between the two hangers; the filter plate is provided with a plurality of placement holes evenly distributed in a lattice shape, and a plug-in ring plate for inserting the tree plant explants is rotatably provided in the placement holes; the filter plate is also provided with a driving mechanism for driving the plurality of plug-in ring plates to rotate simultaneously.

[0008] A flushing component for flushing the explant with sterile water and a drying component for drying the explant with hot air are slidably disposed at both ends of the top surface of the box body. When in use, the flushing component and the drying component are slid to the top of the immersion sterilization tank respectively.

[0009] As a further optimization scheme of the utility model, an inverted U-shaped lifting plate is fixedly provided on the top of the filter plate, and sliding seats are fixedly provided at both ends of the top of the lifting plate. A hanging support plate is slidably provided in the sliding seat, and the hanging support plate is overlapped on the horizontal plate of the hanging bracket.

[0010] As a further optimization scheme of the utility model, fixed seats are fixed at both ends of the top of the plug-in ring plate, a movable rod is provided inside the fixed seat and a clamping wheel frame is fixed at the end of the movable rod, and a spring is provided on the movable rod between the clamping wheel frame and the fixed seat; the two clamping wheel frames clamp the explant in the plug-in ring plate.

[0011] As a further optimization scheme of the utility model, the driving mechanism includes a motor fixed on the top of the lifting plate and a transmission shaft fixed on the output shaft of the motor, and the end of the transmission shaft is fixedly sleeved with a driving gear; the driving mechanism also includes a plurality of driven gears respectively fixedly sleeved on corresponding plug-in ring plates, and the adjacent driven gears in the same row are meshed with each other, and the two rows of driven gears are driven to rotate by the driving gears.

[0012] As a further optimization scheme of the utility model, the flushing assembly includes a flushing box and a flushing pipe fixed to the upper end of the flushing box; the flushing pipe is provided with a plurality of flushing nozzles distributed at equal intervals, and the lower end of the flushing box is provided with a liquid storage chamber.

[0013] As a further optimization solution of the utility model, slide rails are fixedly provided at both ends of the top surface of the box body, and the flushing box is slidably connected to the slide rails; a storage drawer is provided on the front side of the box body below the slide rails.

[0014] The beneficial effects of the utility model are:

[0015] 1. The utility model can insert a large number of North American juniper explants on the filter plate at one time by arranging a filter plate and a plug-in ring plate, so that the device can sterilize multiple North American juniper explants at the same time, and the placement is convenient and fast, and can adapt to the placement of North American juniper explants of different thicknesses, thereby greatly improving the experimental efficiency.

[0016] 2. The utility model provides an immersion sterilization tank, a flushing component, a drying component and a driving mechanism. The disinfectant is first used for immersion sterilization, and then the residual disinfectant on the North American juniper explant is flushed clean by sterile water. Finally, the flushing liquid on the North American juniper explant is quickly dried by hot air, thereby ensuring that the North American juniper explant is fully sterilized during the tissue culture process. The setting of the driving mechanism enables the explant to fully contact with the disinfectant during immersion sterilization, and to fully contact with sterile water and hot air during flushing and drying, thereby improving the sterilization effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional schematic diagram of the overall structure of the utility model;

[0018] Figure 2 It is a schematic diagram of the connection between the filter plate, the plug-in ring plate and the drive mechanism of the utility model;

[0019] Figure 3 This is a schematic diagram of the top surface structure of the plug-in ring plate of the utility model;

[0020] Figure 4 This is a schematic diagram of the bottom surface structure of the plug-in ring plate of the utility model;

[0021] Figure 5 This is a schematic diagram of the box structure of the utility model;

[0022] Figure 6 This is a schematic diagram of the structure of the flushing component of the utility model.

[0023] In the figure:

[0024] 1. Box body; 101. Soaking and sterilizing tank; 102. Slide rail; 103. Storage drawer; 2. Hanging rack; 3. Filter plate; 301. Placement hole; 302. Lifting plate; 303. Slide seat; 304. Hanging support plate; 4. Plug-in ring plate; 401. Fixed seat; 402. Movable rod; 403. Clamping wheel frame; 404. Spring; 5. Driving mechanism; 501. Motor; 502. Transmission shaft; 503. Driving gear; 504. Driven gear; 6. Flushing assembly; 601. Flushing box; 602. Flushing pipe; 603. Flushing nozzle; 604. Liquid storage chamber; 7. Drying assembly. DETAILED DESCRIPTION

[0025] The present application is further described in detail below in conjunction with the accompanying drawings. It is necessary to point out here that the following specific implementation methods are only used to further illustrate the present application and cannot be understood as limiting the scope of protection of the present application. Technical personnel in this field can make some non-essential improvements and adjustments to the present application based on the above application content.

[0026] Embodiment 1

[0027] In order to solve the problem that the existing North American juniper explant tissue culture sterilization treatment device is difficult to sterilize multiple North American juniper explants at the same time, and the contact between the explants and the disinfectant during sterilization is not thorough enough, please refer to Figure 1-Figure 4The utility model provides a sterilization treatment device for tissue culture of arbor plant explants, comprising a box 1, a soaking and sterilizing tank 101 filled with disinfectant is provided in the middle of the top surface of the box 1, and hangers 2 are fixedly provided on the top surface of the box 1 on both sides of the soaking and sterilizing tank 101, and a filter plate 3 with adjustable height is hung between the two hangers 2; the filter plate 3 is provided with a plurality of placement holes 301 evenly distributed in a lattice shape, and a plug-in ring plate 4 for inserting and inserting the North American arborvitae explants is rotatably provided in the placement holes 301; the filter plate 3 is also provided with a driving mechanism 5 for driving the plurality of plug-in ring plates 4 to rotate simultaneously. A large number of North American arborvitae explants can be inserted on the filter plate 3 at one time, thereby greatly improving the experimental efficiency.

[0028] An inverted U-shaped lifting plate 302 is fixed on the top of the filter plate 3, and a slide seat 303 is fixed on both ends of the top of the lifting plate 302. A hanging support plate 304 is slidably provided in the slide seat 303, and the hanging support plate 304 is overlapped on the horizontal plate of the hanger 2. When the height of the filter plate 3 needs to be adjusted, the lifting plate 302 is lifted, and the hanging support plates 304 on both sides are pulled inward to make the hanging support plates 304 leave the hanger 2, and after the lifting plate 302 is lifted to the desired position, the hanging support plates 304 on both sides are pushed outward to make the hanging support plates 304 re-hook on the horizontal plate of the hanger 2.

[0029] Both ends of the top of the plug-in ring plate 4 are fixed with a fixed seat 401, and a movable rod 402 is movably penetrated in the fixed seat 401, and a clamping wheel frame 403 is fixed at the end of the movable rod 402, and a spring 404 is sleeved on the movable rod 402 between the clamping wheel frame 403 and the fixed seat 401; the two clamping wheel frames 403 clamp the explant in the plug-in ring plate 4. Manually move the clamping wheel frames 403 on both sides to insert the North American cypress explant between the two clamping wheel frames 403, and then release the clamping wheel frames 403. Under the action of the spring 404, the North American cypress explant is clamped to prevent the North American cypress explant from falling off the filter plate 3 when rotating. The explant is taken and placed more conveniently and quickly, and a large number of explants to be sterilized can be placed at one time, which improves the experimental efficiency and also makes it adaptable to the use of North American cypress explants of different thicknesses.

[0030] The driving mechanism 5 includes a motor 501 fixed on the top of the lifting plate 302 and a transmission shaft 502 fixed on the output shaft of the motor 501, and a driving gear 503 is fixedly sleeved on the end of the transmission shaft 502; the driving mechanism 5 also includes a plurality of driven gears 504 respectively fixedly sleeved on the corresponding plug-in ring plates 4, and the adjacent driven gears 504 in the same row are meshed with each other, and the two rows of driven gears 504 are driven to rotate by the driving gears 503. When the motor 501 is started, the motor 501 drives the transmission shaft 502 to rotate, the transmission shaft 502 drives the driving gear 503 to rotate, the driving gear 503 drives the two rows of driven gears 504 to rotate, the driven gears 504 respectively drive their respective plug-in ring plates 4 to rotate, and the plug-in ring plates 4 drive the North American cypress explants therein to rotate, so that they can fully contact with the disinfectant during immersion sterilization, and can fully contact with sterile water and hot air during rinsing and drying.

[0031] When in use, multiple North American juniper explants to be sterilized are inserted into the plug-in ring plate 4, and then the position of the filter plate 3 is adjusted so that the North American juniper explants are completely immersed in the disinfectant in the immersion sterilization tank 101, and the multiple North American juniper explants are driven to rotate by the driving mechanism 5 so that they are in full contact with the disinfectant.

[0032] Embodiment 2

[0033] On the basis of Example 1, in order to achieve sufficient sterilization effect and improve sterilization efficiency, Figure 1 , Figure 5-Figure 6 As shown, a washing component 6 for washing the explant with sterile water and a drying component 7 for drying the explant with hot air are slidably disposed at both ends of the top surface of the box body 1.

[0034] The flushing assembly 6 includes a flushing box 601 and a flushing pipe 602 fixed to the upper end of the flushing box 601; the flushing pipe 602 is provided with a plurality of flushing nozzles 603 distributed at equal intervals, and the lower end of the flushing box 601 is provided with a liquid storage chamber 604; both ends of the top surface of the box body 1 are fixed with slide rails 102, and the flushing box 601 is slidably connected to the slide rails 102; a storage drawer 103 is provided at the front of the box body 1 below the slide rails 102. When flushing, the filter plate 3 is lifted out of the immersion sterilization tank 101, and the flushing box 601 is pushed to the top of the immersion sterilization tank 101. At this time, the filter plate 3 is located above the liquid storage chamber 604, and the flushing pipe 602 is connected to an external water pump. Sterile water is sprayed through a plurality of flushing nozzles 603 to rinse the disinfectant remaining on the North American cypress explants.

[0035] The structure of the drying component 7 is the same as that of the rinsing component 6, including a drying box and a drying tube fixed at the upper end of the drying box, and a plurality of drying nozzles distributed at equal intervals are arranged on the drying tube. After rinsing, the drying operation is performed, the drying box is pushed to the top of the immersion sterilization tank 101, and the drying tube is externally connected to a hot air blower, and hot air is sprayed through a plurality of drying nozzles to quickly dry the rinsing liquid on the North American cypress explants.

[0036] The above-mentioned embodiment only expresses one implementation method of the utility model, and its description is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the utility model. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model.

Claims

1. A sterilization treatment device for tissue culture of tree plant explants, comprising a housing (1), characterized in that: A soaking and sterilizing tank (101) filled with disinfectant is provided in the middle of the top surface of the box (1), and hanging racks (2) are fixedly provided on the top surfaces of the box (1) on both sides of the soaking and sterilizing tank (101), and a filter plate (3) with adjustable height is hung between the two hanging racks (2); The filter plate (3) is provided with a plurality of placement holes (301) evenly distributed in a dot matrix, and a plug-in ring plate (4) for inserting tree plant explants is rotatably arranged in the placement holes (301); The filter plate (3) is also provided with a driving mechanism (5) for driving a plurality of plug-in ring plates (4) to rotate simultaneously; A flushing component (6) for flushing the explant with sterile water and a drying component (7) for drying the explant with hot air are slidably provided at both ends of the top surface of the box body (1). When in use, the flushing component (6) and the drying component (7) are slid to the top of the immersion sterilization tank (101).

2. The device for sterilizing and treating tree plant explant tissue culture according to claim 1, characterized in that: An inverted U-shaped lifting plate (302) is fixedly provided on the top of the filter plate (3), and sliding seats (303) are fixedly provided at both ends of the top of the lifting plate (302). A hanging support plate (304) is slidably provided inside the sliding seat (303), and the hanging support plate (304) is overlapped on the horizontal plate of the hanging frame (2).

3. The device for sterilizing and treating tree plant explant tissue culture according to claim 1, characterized in that: Both ends of the top of the plug-in ring plate (4) are fixedly provided with a fixed seat (401), a movable rod (402) is movably inserted inside the fixed seat (401), a clamping wheel frame (403) is fixedly provided at the end of the movable rod (402), and a spring (404) is sleeved on the movable rod (402) between the clamping wheel frame (403) and the fixed seat (401); The two clamping wheel frames (403) clamp the explant in the plug-in ring plate (4).

4. The device for sterilizing and treating tree plant explant tissue culture according to claim 2, characterized in that: The driving mechanism (5) comprises a motor (501) fixedly arranged on the top of the lifting plate (302) and a transmission shaft (502) fixedly arranged on the output shaft of the motor (501), and a driving gear (503) is fixedly sleeved on the end of the transmission shaft (502); The driving mechanism (5) further comprises a plurality of driven gears (504) respectively fixedly sleeved on corresponding plug-in ring plates (4), the driven gears (504) adjacent to each other in the same row being meshed with each other, and the driven gears (504) in two rows are driven to rotate by the driving gears (503).

5. The device for sterilizing and treating tree plant explant tissue culture according to claim 1, characterized in that: The flushing assembly (6) comprises a flushing box (601) and a flushing pipe (602) fixed to the upper end of the flushing box (601); The flushing pipe (602) is provided with a plurality of flushing nozzles (603) distributed at equal intervals, and the lower end of the flushing box (601) is provided with a liquid storage chamber (604).

6. The device for sterilizing and treating tree plant explant tissue culture according to claim 5, characterized in that: Slide rails (102) are fixedly provided at both ends of the top surface of the box body (1), and the flushing box (601) is slidably connected to the slide rails (102); A storage drawer (103) is provided on the front of the box body (1) below the slide rail (102).

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