Fluorescent quantitative PCR (Polymerase Chain Reaction) detection kit

Abstract

The utility model discloses a fluorescent quantitative PCR (Polymerase Chain Reaction) detection kit which comprises a kit body, a cover body is arranged at the top end of the kit body, a metal block is arranged at the front end of the cover body, a magnet block is arranged at the bottom end of the metal block, the magnet block is fixed on the outer side of the kit body, a first storage bin and a second storage bin are arranged in the kit body, and a protective cotton lining is mounted in the first storage bin. By arranging the protective cotton lining, the first sponge mat, the second sponge mat, the rubber plate and the sponge strips, the reagent tube is limited, the sponge strips are distributed between the cover body and the rubber plate, the sponge strips have good elasticity and provide supporting force for the rubber plate, and the reagent tube is prevented from being damaged due to the fact that the sponge strips are matched with the distribution of the first sponge mat and the second sponge mat. The reagent tube storage device can be in flexible contact with a reagent tube, limit the position of the reagent tube, prevent the reagent tube from jumping in the first storage bin and protect a reagent, so that the use safety is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of fluorescence quantitative PCR detection kits, in particular to a fluorescence quantitative PCR detection kit. Background Art

[0002] Fluorescence quantitative PCR detection kit is a detection tool for fluorescence quantitative PCR experiments. PCR (polymerase chain reaction) is a molecular biology technique used to amplify specific DNA fragments. Fluorescence quantitative PCR monitors the amount of PCR product generated in real time by adding fluorescent dyes or fluorescently labeled specific probes during the PCR process, thereby achieving quantitative detection of DNA or RNA samples. In the process of pig breeding, fluorescence quantitative PCR detection kits are generally used for swine fever virus detection. African swine fever is an acute, hemorrhagic, and highly contagious disease caused by African swine fever virus infecting domestic pigs and various wild boars (such as African wild boars, European wild boars, etc.). The characteristics of African swine fever are short onset and acute infection mortality rate as high as 100%. In actual use, the fluorescence quantitative PCR detection kit will be carried and transported. Affected by the bumpy factor, the reagent tube will jump inside the box body, causing bumps, and severe reagent leakage. Therefore, a fluorescence quantitative PCR detection kit is now provided. Utility Model Content

[0003] The purpose of the utility model is to provide a fluorescent quantitative PCR detection kit to solve the problems raised in the above background technology.

[0004] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a fluorescent quantitative PCR detection kit, comprising a box body, a cover body is installed on the top of the box body, and a metal block is installed on the front end of the cover body, a magnet block is arranged at the bottom end of the metal block, and the magnet block is fixed on the outside of the box body, a first storage bin and a second storage bin are arranged inside the box body, a protective cotton lining is installed inside the first storage bin, and a reagent tube is arranged inside the protective cotton lining, a sponge strip is installed at the bottom end of the cover body, and a rubber plate is installed at the bottom end of the sponge strip, a first sponge pad and a second sponge pad are installed at the bottom end of the rubber plate, and the first sponge pad and the second sponge pad are respectively arranged at the top of the second storage bin and the top of the first storage bin.

[0005] Preferably, the sponge strips are provided in a plurality of groups, and the sponge strips are arranged and distributed at equal intervals between the cover body and the rubber plate.

[0006] Preferably, a foam lining is provided on the outer side of the box body, and a rubber protective sleeve is provided on the outer side of the foam lining.

[0007] Preferably, a heat-insulating aluminum foil cloth is provided between the foam outer lining and the box body, and the heat-insulating aluminum foil cloth is connected to the box body and the foam outer lining by an adhesive.

[0008] Preferably, a plurality of groups of partitions are arranged inside the second storage bin, and limiting strips are arranged on both sides of the partitions, and the limiting strips are fixed on the inner side wall of the second storage bin.

[0009] Preferably, the limiting bars are provided in a plurality of groups, and the limiting bars are arranged and distributed at equal intervals on the inner wall of the second storage bin, and the intervals between the limiting bars are adapted to the thickness of the partition.

[0010] Compared with the prior art, the beneficial effects of the utility model are:

[0011] (1) By providing a protective cotton lining, a first sponge pad, a second sponge pad, a rubber plate and a sponge strip, the reagent tube is limited. The sponge strip is arranged and distributed between the cover body and the rubber plate. The sponge strip has good elasticity and provides support for the rubber plate. With the distribution of the first sponge pad and the second sponge pad, it can flexibly contact with the reagent tube, limit the position of the reagent tube, prevent the reagent tube from jumping inside the first storage bin, protect the medicine, and thus improve the safety of use;

[0012] (2) The reagent kit is protected by providing a rubber protective sleeve, a foam outer lining, a limit strip and a heat-insulating aluminum foil cloth. The foam outer lining and the limit strip cover the outside of the box body to form a double protective layer, which prevents direct contact between the object and the box body, reduces damage to the box body caused by collision, and protects the reagent kit. The distribution of the foam outer lining and the heat-insulating aluminum foil cloth effectively reduces the influence of the external temperature on the inside of the reagent kit, so that the reagent kit has the function of heat insulation;

[0013] (3) By setting a limit strip, a second storage bin and partitions, the storage of items is achieved. The cooperation between the limit strip and the partition can divide the second storage bin into multiple spaces for storing multiple groups of items. The distribution of the limit strips facilitates flexible adjustment of the space size, thereby improving the flexibility of use. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] Figure 1 This is a front view structural schematic diagram of the utility model;

[0016] Figure 2 It is a side view structural schematic diagram of the utility model;

[0017] Figure 3 It is a front view cross-sectional structural schematic diagram of the utility model;

[0018] Figure 4 For the utility model Figure 3 Enlarged structural diagram at A in the middle.

[0019] Description of reference numerals in the figures:

[0020] 1. Box body; 2. Cover body; 3. Metal block; 4. Magnet block; 5. Rubber protective cover; 6. First storage compartment; 7. Reagent tube; 8. Protective cotton lining; 9. Foam outer lining; 10. Limiting strip; 11. Second storage compartment; 12. Partition; 13. First sponge pad; 14. Second sponge pad; 15. Rubber plate; 16. Sponge strip; 17. Insulating aluminum foil cloth. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the embodiment of the utility model clearer, the technical solution in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is a part of the embodiment of the utility model, not all the embodiments. Based on the embodiment of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0023] See also Figure 1-4, the utility model provides an embodiment: a fluorescent quantitative PCR detection kit, comprising a box body 1, a cover body 2 is installed on the top of the box body 1, and a metal block 3 is installed at the front end of the cover body 2, a magnet block 4 is arranged at the bottom end of the metal block 3, and the magnet block 4 is fixed on the outside of the box body 1, a first storage bin 6 and a second storage bin 11 are arranged inside the box body 1, a protective cotton lining 8 is installed inside the first storage bin 6, and a reagent tube 7 is arranged inside the protective cotton lining 8, a sponge strip 16 is installed at the bottom end of the cover body 2, and a rubber plate 15 is installed at the bottom end of the sponge strip 16, a first sponge pad 13 and a second sponge pad 14 are installed at the bottom end of the rubber plate 15, and the first sponge pad 13 and the second sponge pad 14 are respectively arranged at the top of the second storage bin 11 and the first storage bin 6, a plurality of groups of sponge strips 16 are provided, and the sponge strips 16 are arranged and distributed at equal intervals between the cover body 2 and the rubber plate 15, and the sponge strips 16 can provide support for the rubber plate 15 and limit the top of the reagent tube 7;

[0024] Specifically, as shown in the figure, when in use, the elasticity of the sponge strip 16 supports the rubber plate 15, and with the distribution of the first sponge pad 13 and the second sponge pad 14, it can flexibly contact with the reagent tube 7, limit the position of the reagent tube 7, prevent the reagent tube 7 from jumping inside the first storage bin 6, and protect the medicine;

[0025] A foam lining 9 is arranged on the outside of the box body 1, and a rubber protective cover 5 is arranged on the outside of the foam lining 9. A heat-insulating aluminum foil cloth 17 is arranged between the foam lining 9 and the box body 1, and the heat-insulating aluminum foil cloth 17 is connected to the box body 1 and the foam lining 9 by an adhesive. The distribution of the foam lining 9 and the heat-insulating aluminum foil cloth 17 effectively reduces the influence of the external temperature on the inside of the reagent box.

[0026] Specifically, as shown in the figure, when in use, the outer side of the box body 1 is covered by the foam outer lining 9 and the limit strip 10, forming a double protective layer, preventing direct contact between the object and the box body 1, reducing the damage to the box body 1 caused by collision, and protecting the reagent kit;

[0027] A plurality of groups of partitions 12 are arranged inside the second storage bin 11, and limit bars 10 are arranged on both sides of the partitions 12. The limit bars 10 are fixed on the inner wall of the second storage bin 11. A plurality of groups of limit bars 10 are arranged and distributed at equal intervals on the inner wall of the second storage bin 11. The intervals between the limit bars 10 are adapted to the thickness of the partitions 12, so as to limit the position of the partitions 12, and the partitions 12 are vertically installed inside the second storage bin 11.

[0028] Specifically, as shown in the figure, when in use, the second storage compartment 11 can be divided into multiple spaces through the cooperation of the limit strips 10 and the partitions 12 to store multiple groups of items, and the distribution of the limit strips 10 facilitates flexible adjustment of the space size.

[0029] Working principle: When in use, first, carry the fluorescent quantitative PCR detection kit to the target area. When the reagent tube 7 needs to be taken out, grab the box body 1 and the cover body 2 with both hands respectively, control the cover body 2 to flip upward, separate the metal block 3 from the top of the magnet block 4, open the fluorescent quantitative PCR detection kit, and take out the reagent tubes 7 in turn. Then, when the items need to be stored, control the partition 12 to move to the appropriate position and insert it between the corresponding limit bars 10, divide the second storage compartment 11 into multiple groups of placement slots, and store multiple groups of items. Then, when the fluorescent quantitative PCR detection kit needs to be carried or transported, the cover 2 is controlled to cover the top of the box body 1, and the magnetism of the magnet block 4 will adsorb the metal block 3 to lock the position of the cover 2. The elasticity of the sponge strip 16 supports the rubber plate 15, and with the distribution of the first sponge pad 13 and the second sponge pad 14, it can flexibly contact with the reagent tube 7 to limit the position of the reagent tube 7 and prevent the reagent tube 7 from jumping inside the first storage bin 6, thereby protecting the agent and finally completing the use of the fluorescent quantitative PCR detection kit.

[0030] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A fluorescent quantitative PCR detection kit, comprising a box body (1), characterized in that: The top of the box body (1) is provided with a cover body (2), and the front end of the cover body (2) is provided with a metal block (3), the bottom end of the metal block (3) is provided with a magnet block (4), and the magnet block (4) is fixed to the outside of the box body (1), the inside of the box body (1) is provided with a first storage bin (6) and a second storage bin (11), the inside of the first storage bin (6) is provided with a protective cotton lining (8), and the inside of the protective cotton lining (8) is provided with a reagent tube (7), the bottom end of the inside of the cover body (2) is provided with a sponge bar (16), and the bottom end of the sponge bar (16) is provided with a rubber plate (15), the bottom end of the rubber plate (15) is provided with a first sponge pad (13) and a second sponge pad (14), and the first sponge pad (13) and the second sponge pad (14) are respectively provided at the top of the second storage bin (11) and the top of the first storage bin (6).

2. A fluorescent quantitative PCR detection kit according to claim 1, characterized in that: The sponge strips (16) are arranged in a plurality of groups, and the sponge strips (16) are arranged and distributed at equal intervals between the cover body (2) and the rubber plate (15).

3. A fluorescent quantitative PCR detection kit according to claim 1, characterized in that: The outer side of the box body (1) is provided with a foam outer lining (9), and the outer side of the foam outer lining (9) is provided with a rubber protective sleeve (5).

4. A fluorescent quantitative PCR detection kit according to claim 3, characterized in that: A heat-insulating aluminum foil cloth (17) is provided between the foam outer lining (9) and the box body (1), and the heat-insulating aluminum foil cloth (17) is connected to the box body (1) and the foam outer lining (9) by means of an adhesive.

5. A fluorescent quantitative PCR detection kit according to claim 1, characterized in that: A plurality of groups of partitions (12) are arranged inside the second storage bin (11), and limit strips (10) are arranged on both sides of the partitions (12), and the limit strips (10) are fixed on the inner side wall of the second storage bin (11).

6. A fluorescent quantitative PCR detection kit according to claim 5, characterized in that: The limiting strips (10) are provided in a plurality of groups, and the limiting strips (10) are arranged and distributed at equal intervals on the inner wall of the second storage bin (11), and the intervals between the limiting strips (10) are adapted to the thickness of the partition (12).

Images