Novel pseudomonas fluorescens fermentation shaking table

Abstract

The utility model relates to the technical field of biological pharmacy, in particular to a novel pseudomonas fluorescens fermentation shaking table which reduces pollution of bacteria and impurities in air to a culture solution, ensures the fermentation rate of pseudomonas fluorescens in the culture solution, and is convenient to use and high in practicability. Comprising a bottom plate, a fermentation box and a sealing door, the fermentation box is fixedly installed at the upper end of the bottom plate, a cavity is formed in the fermentation box, an opening is formed in the front end of the fermentation box, and the sealing door is installed at the opening in the front end of the fermentation box; the device further comprises supporting plates, multiple sets of culture bottles, an air purification and ventilation mechanism, multiple sets of clamping mechanisms and a shaking mechanism, the supporting plates are installed in the cavity of the fermentation box through the shaking mechanism, the shaking mechanism is used for shaking the supporting plates, the multiple sets of clamping mechanisms are all installed on the supporting plates, and the multiple sets of culture bottles are clamped and fixed by the multiple sets of supporting plates respectively. Culture solutions are arranged in the multiple groups of culture bottles, and the air purification and ventilation mechanism is mounted on the fermentation box and has an air purification effect.

Description

Technical Field

[0001] The utility model relates to the technical field of biopharmaceuticals, in particular to a novel Pseudomonas fluorescens fermentation shaker. Background Art

[0002] Pseudomonas fluorescens is an aerobic organism, and its metabolite diketo-D-gluconic acid is an intermediate in the production process of sodium isoVC. When producing sodium isoVC, Pseudomonas fluorescens needs to be fermented and grown in the culture medium of the culture bottle, and the culture bottle needs to be placed on a fermentation shaker; in the prior art, a utility model patent with patent application number 202220355502.6 discloses a high-efficiency shaker, which mainly includes a shell, a movable plate and a clamping device on the movable plate, and the movable plate is installed in the shell; when in use, multiple culture bottles are clamped and fixed by multiple clamping devices on the movable plate, and then the bacteria to be cultured are inoculated into the culture medium of multiple culture bottles. Since Pseudomonas fluorescens Bacteria are aerobic organisms, so the culture bottle needs to be kept open to allow outside air to enter the culture bottle, and then the bacteria can be fermented and grown in the culture solution; the inventor believes that there are the following problems during use: when the bacteria are fermented, the outside air can directly contact the culture solution in the culture bottle, and impurities such as bacteria and dust in the air will pollute the culture solution, resulting in reduced fermentation efficiency of fluorescent Pseudomonas, low yield, and inconvenience in use. Therefore, a fermentation shaker that can ensure the fermentation efficiency of fluorescent Pseudomonas is needed. Utility Model Content

[0003] In order to solve the above technical problems, the utility model provides a novel Pseudomonas fluorescens fermentation shaker which reduces the pollution of bacteria and impurities in the air to the culture solution, ensures the fermentation rate of Pseudomonas fluorescens in the culture solution, is easy to use and has high practicality.

[0004] The utility model discloses a novel fluorescent pseudomonas fermentation shaker, comprising a bottom plate, a fermentation box and a sealed door, wherein the fermentation box is fixedly mounted on the upper end of the bottom plate, a chamber is arranged in the fermentation box, an opening is arranged at the front end of the fermentation box, and the sealed door is mounted at the opening at the front end of the fermentation box; the utility model also comprises a support plate, multiple groups of culture bottles, an air purification ventilation mechanism, multiple groups of clamping mechanisms and a shaking mechanism, wherein the support plate is mounted in the chamber of the fermentation box through the shaking mechanism, the shaking mechanism is used to shake the support plate, the multiple groups of clamping mechanisms are all mounted on the support plate, the multiple groups of culture bottles are clamped and fixed by the multiple groups of support plates respectively, the multiple groups of culture bottles are all provided with culture solution, the air purification ventilation mechanism is mounted on the fermentation box, and the air purification ventilation mechanism has the function of purifying the air; in shaking the fluorescent pseudomonas When the Pseudomonas fluorescens is fermented, the strains of Pseudomonas fluorescens are inoculated into multiple groups of culture bottles respectively, and then the support plate is shaken by a shaking mechanism, and the support plate shakes the multiple groups of culture bottles through a clamping mechanism. During the shaking process, the strains of Pseudomonas fluorescens are fully dispersed into the culture solution of the multiple groups of culture bottles, and at the same time, the outside air is sterilized and purified by the air purification and ventilation mechanism and then enters the fermentation box chamber for use by the Pseudomonas fluorescens fermentation; the air purification effect of the air purification and ventilation mechanism on the air makes the air entering the fermentation box chamber be sterilized and purified, thereby reducing the contamination of the culture solution by bacteria and impurities in the air, ensuring the fermentation rate of Pseudomonas fluorescens in the culture solution, and the method is easy to use and highly practical.

[0005] Preferably, the air purification ventilation mechanism includes an exhaust pipe, a one-way valve, an induced draft fan, an air intake pipe, a delivery pipe, a plasma air sterilizer and an air delivery pipe. The exhaust pipe is installed on the fermentation box, the input end of the exhaust pipe is connected to the left part of the fermentation box chamber, and a one-way valve is arranged on the exhaust pipe. The induced draft fan is fixedly installed on the bottom plate, the input end of the induced draft fan is provided with an air intake pipe, the output end of the induced draft fan is connected to the plasma air sterilizer through the delivery pipe, the output end of the plasma air sterilizer is connected to the right part of the fermentation box chamber through the air delivery pipe, the fermentation box is provided with an oxygen concentration monitor, and the air intake pipe is provided with a filter screen; due to the fermentation of Pseudomonas fluorescens, the fermentation box chamber contains When the oxygen concentration is low, turn on the induced draft fan, so that the outside air is filtered out impurities through the filter on the air inlet pipe, and then enters the plasma air sterilizer through the induced draft fan and the delivery pipe to be sterilized. After that, the air sterilized by the plasma air sterilizer enters the fermentation box through the air delivery pipe, and the original air in the fermentation box chamber can be discharged through the exhaust pipe; because the outside air is filtered by the filter and sterilized by the plasma air sterilizer before entering the chamber of the fermentation box for the fermentation of Pseudomonas fluorescens, the pollution of the culture solution by bacteria and impurities in the air is reduced, and the fermentation rate of Pseudomonas fluorescens in the culture solution is guaranteed. It is easy to use and highly practical.

[0006] Preferably, the clamping mechanism includes a support plate, a lead screw, two groups of sliders and two groups of clamping plates. The support plate is fixedly mounted on the upper end of the support plate, the lead screw is rotatably mounted on the support plate, the left and right parts of the lead screw are respectively provided with external threads of opposite rotation directions, the two groups of sliders are respectively screwed with the left and right parts of the lead screw, the two groups of sliders are both slidably mounted on the support plate, the two groups of clamping plates are respectively fixedly mounted on the upper ends of the two groups of sliders, the culture bottle is clamped and fixed by the two groups of clamping plates, and the culture bottle is placed on the support plate; the clamping and fixing of the culture bottle by the two groups of clamping plates ensures the stability of the culture bottle during the shaking process, and because the distance between the two groups of clamping plates can be adjusted, it can clamp and fix culture bottles of different diameters, is easy to use, and has low limitations.

[0007] Preferably, the shaking mechanism includes a moving mechanism, a driving mechanism, two groups of fixed plates, two groups of sliding blocks, four groups of springs A, two groups of sliding plates and two groups of springs B. The rear ends of the two groups of fixed plates are installed on the moving mechanism. The moving mechanism is used to move the two groups of fixed plates forward and backward. Slide rails are provided in the two groups of fixed plates. The two groups of sliding blocks are respectively slidably installed in the slide rails of the two groups of fixed plates. The front and rear ends of the two groups of sliding blocks are fixedly installed on a group of springs A. The other ends of the four groups of springs A are respectively fixedly installed on the front and rear ends of the two groups of fixed plates. The left and right ends of the two groups of sliding plates are respectively fixedly installed on the two groups of sliding blocks. Two groups of sliding grooves that are connected to the left and right are provided on the support plate. The support plate is connected to the two groups of sliding grooves through the two groups of sliding grooves. The two sets of sliding plates are connected for sliding left and right, the support plates are installed on the two sets of sliding plates for sliding left and right, one end of the two sets of springs B are fixedly installed on the left and right ends of the support plates respectively, the other ends of the two sets of springs B are fixedly installed on the two sets of sliding blocks respectively, the driving mechanism is installed at the lower end of the support plate, and the driving mechanism is used to provide power for shaking the support plate; when shaking the support plate, the driving mechanism is turned on, and the driving mechanism applies a shaking force to the support plate, and then the support plate periodically moves left and right and forward and backward. During the shaking process, the support plate causes the multiple sets of culture bottles above the support plate to shake, which promotes the shaking of the culture solution in the multiple sets of culture bottles, promotes the dispersion of the strains in the culture solution, increases the contact area between the strains and the culture solution, and increases the fermentation rate.

[0008] Preferably, the driving mechanism includes a support frame, a servo motor, a rotating shaft and an eccentric block, the servo motor and the support frame are fixedly mounted on the lower end of the support plate, the rotating shaft is rotatably mounted on the support frame, the upper end of the rotating shaft is connected to the output end of the servo motor, and the eccentric block is fixedly mounted on the lower end of the rotating shaft; when the support plate is shaken, the servo motor is turned on, and the servo motor rotates the eccentric block through the rotating shaft, and the centrifugal force generated by the eccentric block during the rotation process is transmitted to the support plate through the rotating shaft and the support frame, thereby causing the support plate to shake back and forth and left and right; this facilitates the shaking of the support plate.

[0009] Preferably, the moving mechanism includes a connecting plate, a sliding rod, a push rod and a hydraulic cylinder, the connecting plate is fixedly mounted on the rear end of the two groups of fixed plates, the connecting plate is located in the chamber of the fermentation box, the sliding rod and the push rod are both slidably mounted on the fermentation box forward and backward, the rear end of the push rod is connected to the output end of the hydraulic cylinder, and the hydraulic cylinder is fixedly mounted on the rear end of the fermentation box; when the fermentation is completed, when it is necessary to collect multiple groups of culture bottles above the support plate, the sealing door is opened, and then the hydraulic cylinder is opened, the hydraulic cylinder moves the connecting plate forward through the push rod, and the connecting plate drives the two groups of sliding blocks to move the support plate forward through the two groups of fixed plates until the support plate is moved out through the opening at the front end of the fermentation box, and then the staff can remove the multiple groups of culture bottles above the support plate; it is convenient to move multiple groups of culture bottles and improves convenience.

[0010] Preferably, the fermentation box is provided with a thermometer and hygrometer; through the above arrangement, it is convenient to monitor the temperature and humidity in the fermentation box chamber, facilitate the monitoring of the fluorescein monocytogenes fermentation environment, and improve convenience.

[0011] Compared with the prior art, the utility model has the following beneficial effects: reducing the pollution of the culture solution by bacteria and impurities in the air, ensuring the fermentation rate of Pseudomonas fluorescens in the culture solution, being easy to use and having high practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the axonometric structure of the utility model;

[0013] Figure 2 It is a cross-sectional structural schematic diagram of the utility model;

[0014] Figure 3 It is a structural schematic diagram of a support plate, a push rod and a sliding rod;

[0015] Figure 4 It is a schematic diagram of the structure of the eccentric block, servo motor and rotating shaft;

[0016] Figure 5 It is a structural schematic diagram of the clamping mechanism.

[0017] Markings in the attached drawings: 1. bottom plate; 2. fermentation box; 3. sealing door; 4. support plate; 5. culture bottle; 6. exhaust pipe; 7. one-way valve; 8. induced draft fan; 9. air inlet pipe; 10. delivery pipe; 11. plasma air sterilizer; 12. air delivery pipe; 13. support plate; 14. lead screw; 15. slider; 16. splint; 17. fixed plate; 18. sliding block; 19. spring A; 20. sliding plate; 21. spring B; 22. support frame; 23. servo motor; 24. rotating shaft; 25. eccentric block; 26. connecting plate; 27. sliding rod; 28. push rod; 29. ​​hydraulic cylinder. DETAILED DESCRIPTION

[0018] In order to facilitate the understanding of the utility model, the utility model will be described more comprehensively below with reference to the relevant drawings. The utility model can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the utility model more thorough and comprehensive. Example 1

[0019] like Figures 1 to 5 The fermentation shaker of the utility model comprises a bottom plate 1, a fermentation box 2, a sealing door 3, a support plate 4, multiple groups of culture bottles 5, an air purification ventilation mechanism, multiple groups of clamping mechanisms and a shaking mechanism. The fermentation box 2 is fixedly installed on the upper end of the bottom plate 1, a chamber is arranged in the fermentation box 2, an opening is arranged at the front end of the fermentation box 2, the sealing door 3 is installed at the opening at the front end of the fermentation box 2, the support plate 4 is installed in the chamber of the fermentation box 2 through a shaking mechanism, the shaking mechanism is used to shake the support plate 4, the multiple groups of clamping mechanisms are all installed on the support plate 4, the multiple groups of culture bottles 5 are clamped and fixed by the multiple groups of support plates 4, the multiple groups of culture bottles 5 are all provided with culture solution, the air purification ventilation mechanism is installed on the fermentation box 2, and the air purification ventilation mechanism has the function of purifying the air; when shaking the fluorescent pseudomonas When the Pseudomonas fluorescens is fermented, the strains of Pseudomonas fluorescens are inoculated into multiple groups of culture bottles 5 respectively, and then the support plate 4 is shaken by the shaking mechanism. The support plate 4 shakes the multiple groups of culture bottles 5 through the clamping mechanism. During the shaking process, the strains of Pseudomonas fluorescens are fully dispersed into the culture solution of the multiple groups of culture bottles 5. At the same time, the outside air is sterilized and purified by the air purification and ventilation mechanism and then enters the chamber of the fermentation box 2 for use by the Pseudomonas fluorescens fermentation; the air purification effect of the air purification and ventilation mechanism on the air makes the air entering the chamber of the fermentation box 2 sterilized and purified, reduces the contamination of the culture solution by bacteria and impurities in the air, and ensures the fermentation rate of Pseudomonas fluorescens in the culture solution. The utility model is convenient to use and has high practicality.

[0020] like Figure 2The air purification ventilation mechanism includes an exhaust pipe 6, a one-way valve 7, an induced draft fan 8, an air intake pipe 9, a delivery pipe 10, a plasma air sterilizer 11 and an air delivery pipe 12. The exhaust pipe 6 is installed on the fermentation box 2. The input end of the exhaust pipe 6 is connected to the left part of the chamber of the fermentation box 2. The exhaust pipe 6 is provided with a one-way valve 7. The induced draft fan 8 is fixedly installed on the bottom plate 1. The input end of the induced draft fan 8 is provided with an air intake pipe 9. The output end of the induced draft fan 8 is connected to the plasma air sterilizer 11 through the delivery pipe 10. The output end of the plasma air sterilizer 11 is connected to the right part of the chamber of the fermentation box 2 through the air delivery pipe 12. The fermentation box 2 is provided with an oxygen concentration monitor, and the air intake pipe 9 is provided with a filter. Due to the fermentation of Pseudomonas fluorescens, the fermentation When the oxygen concentration in the chamber of the box 2 is low, the induced draft fan 8 is turned on to allow the external air to filter out impurities through the filter on the air inlet pipe 9 and then enter the plasma air sterilizer 11 through the induced draft fan 8 and the delivery pipe 10 to be sterilized. Thereafter, the air sterilized by the plasma air sterilizer 11 enters the fermentation box 2 through the air delivery pipe 12, and the original air in the chamber of the fermentation box 2 can be discharged through the exhaust pipe 6. Since the external air is filtered by the filter and sterilized by the plasma air sterilizer 11 before entering the chamber of the fermentation box 2 for use by the fluorescent pseudomonas fermentation, the pollution of the culture solution by bacteria and impurities in the air is reduced, and the fermentation rate of the fluorescent pseudomonas in the culture solution is guaranteed. The use is convenient and the practicability is high.

[0021] like Figure 5 The clamping mechanism includes a support plate 13, a lead screw 14, two groups of sliders 15 and two groups of clamping plates 16. The support plate 13 is fixedly mounted on the upper end of the support plate 4, the lead screw 14 is rotatably mounted on the support plate 13, the left and right parts of the lead screw 14 are respectively provided with external threads with opposite rotation directions, the two groups of sliders 15 are respectively screwed with the left and right parts of the lead screw 14, the two groups of sliders 15 are both slidably mounted on the support plate 13, the two groups of clamping plates 16 are respectively fixedly mounted on the upper ends of the two groups of sliders 15, the culture bottle 5 is clamped and fixed by the two groups of clamping plates 16, and the culture bottle 5 is placed on the support plate 13; the clamping and fixing of the culture bottle 5 by the two groups of clamping plates 16 ensures the stability of the culture bottle 5 during the shaking process, and because the distance between the two groups of clamping plates 16 can be adjusted, it can clamp and fix the culture bottles 5 of different diameters, which is convenient to use and has low limitations.

[0022] like Figure 3 and Figure 4The shaking mechanism includes a moving mechanism, a driving mechanism, two groups of fixed plates 17, two groups of sliding blocks 18, four groups of springs A19, two groups of sliding plates 20 and two groups of springs B21. The rear ends of the two groups of fixed plates 17 are installed on the moving mechanism. The moving mechanism is used to move the two groups of fixed plates 17 forward and backward. Slide rails are provided in the two groups of fixed plates 17. The two groups of sliding blocks 18 are respectively slidably installed in the slide rails of the two groups of fixed plates 17. The front and rear ends of the two groups of sliding blocks 18 are fixedly installed on a group of springs A19. The other ends of the four groups of springs A19 are respectively fixedly installed on the front and rear ends of the two groups of fixed plates 17. The left and right ends of the two groups of sliding plates 20 are respectively fixedly installed on the two groups of sliding blocks 18. Two groups of sliding grooves that are connected to the left and right are provided on the support plate 4. The support plate 4 passes through two groups of sliding grooves. The slide groove is connected to the two groups of sliding plates 20 for sliding left and right movement. The support plate 4 is installed on the two groups of sliding plates 20 for sliding left and right movement. One end of the two groups of springs B21 are fixedly installed on the left and right ends of the support plate 4 respectively. The other ends of the two groups of springs B21 are fixedly installed on the two groups of sliding blocks 18 respectively. The driving mechanism is installed at the lower end of the support plate 4. The driving mechanism is used to provide power for the shaking of the support plate 4. When the support plate 4 is shaken, the driving mechanism is turned on, and the driving mechanism applies a shaking force to the support plate 4. Thereafter, the support plate 4 periodically moves left and right and forward and backward. During the shaking process, the support plate 4 causes the multiple groups of culture bottles 5 above the support plate 4 to shake, thereby promoting the shaking of the culture solution in the multiple groups of culture bottles 5, promoting the dispersion of the strains in the culture solution, increasing the contact area between the strains and the culture solution, and increasing the fermentation rate.

[0023] like Figure 4 The driving mechanism includes a support frame 22, a servo motor 23, a rotating shaft 24 and an eccentric block 25. The servo motor 23 and the support frame 22 are fixedly mounted at the lower end of the support plate 4. The rotating shaft 24 is rotatably mounted on the support frame 22. The upper end of the rotating shaft 24 is connected to the output end of the servo motor 23. The eccentric block 25 is fixedly mounted at the lower end of the rotating shaft 24. The eccentric block 25 is eccentrically connected to the rotating shaft 24. When the support plate 4 is shaken, the servo motor 23 is turned on. The servo motor 23 rotates the eccentric block 25 through the rotating shaft 24. The centrifugal force generated by the eccentric block 25 during the rotation process is transmitted to the support plate 4 through the rotating shaft 24 and the support frame 22, thereby causing the support plate 4 to shake back and forth and left and right. This facilitates the shaking of the support plate 4.

[0024] like Figure 3The moving mechanism includes a connecting plate 26, a sliding rod 27, a push rod 28 and a hydraulic cylinder 29. The connecting plate 26 is fixedly installed at the rear end of the two groups of fixed plates 17. The connecting plate 26 is located in the chamber of the fermentation box 2. The sliding rod 27 and the push rod 28 are both slidably installed on the fermentation box 2 forward and backward. The rear end of the push rod 28 is connected to the output end of the hydraulic cylinder 29. The hydraulic cylinder 29 is fixedly installed at the rear end of the fermentation box 2; when the fermentation is completed, it is necessary to collect the multiple groups of culture bottles 5 above the support plate 4, open the sealing door 3, and then open the hydraulic cylinder 29. The hydraulic cylinder 29 moves the connecting plate 26 forward through the push rod 28. The connecting plate 26 drives the two groups of sliding blocks 18 to move forward through the two groups of fixed plates 17 until the support plate 4 is moved out through the opening at the front end of the fermentation box 2. Then the staff can take out the multiple groups of culture bottles 5 above the support plate 4; it is convenient to move the multiple groups of culture bottles 5 and improve the convenience. Example 2

[0025] On the basis of Example 1, a thermometer and hygrometer are additionally provided on the fermentation box 2; through the above arrangement, the monitoring of the temperature and humidity in the chamber of the fermentation box 2 is facilitated, the monitoring of the fermentation environment of Fluoromonas is facilitated, and the convenience is improved.

[0026] The culture bottle 5, induced draft fan 8, plasma air sterilizer 11, lead screw 14, eccentric block 25 and hydraulic cylinder 29 of the novel fluorescent Pseudomonas fermentation shaker of the utility model are all purchased on the market. The technicians in this industry only need to install and operate them according to the accompanying instruction manual, without the need for the technicians in this field to make creative efforts.

[0027] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A novel Pseudomonas fluorescens fermentation shaker, comprising a bottom plate (1), a fermentation box (2) and a sealing door (3), wherein the fermentation box (2) is fixedly mounted on the upper end of the bottom plate (1), a chamber is arranged inside the fermentation box (2), an opening is arranged at the front end of the fermentation box (2), and the sealing door (3) is mounted at the opening at the front end of the fermentation box (2); characterized in that: The invention also comprises a support plate (4), a plurality of groups of culture bottles (5), an air purification and ventilation mechanism, a plurality of groups of clamping mechanisms and a shaking mechanism. The support plate (4) is installed in the chamber of the fermentation box (2) via the shaking mechanism. The shaking mechanism is used to shake the support plate (4). The plurality of groups of clamping mechanisms are all installed on the support plate (4). The plurality of groups of culture bottles (5) are respectively clamped and fixed by the plurality of groups of support plates (4). The plurality of groups of culture bottles (5) are all provided with culture fluid. The air purification and ventilation mechanism is installed on the fermentation box (2). The air purification and ventilation mechanism has the function of purifying air.

2. A novel Pseudomonas fluorescens fermentation shaker as claimed in claim 1, characterized in that: The air purification ventilation mechanism comprises an exhaust pipe (6), a one-way valve (7), an induced draft fan (8), an air intake pipe (9), a delivery pipe (10), a plasma air sterilizer (11) and an air delivery pipe (12). The exhaust pipe (6) is mounted on the fermentation box (2). The input end of the exhaust pipe (6) is connected to the left part of the chamber of the fermentation box (2). The exhaust pipe (6) is provided with a one-way valve (7). The induced draft fan (8) is fixedly mounted on the bottom plate (1). The input end of the induced draft fan (8) is provided with an air intake pipe (9). The output end of the induced draft fan (8) is connected to the plasma air sterilizer (11) through the delivery pipe (10). The output end of the plasma air sterilizer (11) is connected to the right part of the chamber of the fermentation box (2) through the air delivery pipe (12). The fermentation box (2) is provided with an oxygen concentration monitor. The air intake pipe (9) is provided with a filter screen.

3. A novel Pseudomonas fluorescens fermentation shaker as claimed in claim 1, characterized in that: The clamping mechanism comprises a support plate (13), a lead screw (14), two groups of sliders (15) and two groups of clamping plates (16); the support plate (13) is fixedly mounted on the upper end of the support plate (4); the lead screw (14) is rotatably mounted on the support plate (13); the left and right parts of the lead screw (14) are respectively provided with external threads with opposite rotation directions; the two groups of sliders (15) are respectively screwed to the left and right parts of the lead screw (14); the two groups of sliders (15) are both slidably mounted on the support plate (13) left and right; the two groups of clamping plates (16) are respectively fixedly mounted on the upper ends of the two groups of sliders (15); the culture bottle (5) is clamped and fixed by the two groups of clamping plates (16); and the culture bottle (5) is placed on the support plate (13).

4. A novel Pseudomonas fluorescens fermentation shaker as claimed in claim 1, characterized in that: The shaking mechanism comprises a moving mechanism, a driving mechanism, two groups of fixed plates (17), two groups of sliding blocks (18), four groups of springs A (19), two groups of sliding plates (20) and two groups of springs B (21). The rear ends of the two groups of fixed plates (17) are mounted on the moving mechanism. The moving mechanism is used to move the two groups of fixed plates (17) forward and backward. The two groups of fixed plates (17) are provided with sliding rails. The two groups of sliding blocks (18) are respectively mounted in the sliding rails of the two groups of fixed plates (17) for sliding forward and backward. The front and rear ends of the two groups of sliding blocks (18) are fixedly mounted on a group of springs A (19). The other ends of the four groups of springs A (19) are respectively fixedly mounted on the two groups of fixed plates. The front and rear ends of the two fixed plates (17) are fixedly mounted on the two sets of sliding blocks (18) at the left and right ends of the two sets of sliding plates (20). The support plate (4) is provided with two sets of sliding grooves communicating with each other. The support plate (4) is slidably connected to the two sets of sliding plates (20) via the two sets of sliding grooves. The support plate (4) is slidably mounted on the two sets of sliding plates (20) at the left and right ends. One ends of the two sets of springs B (21) are fixedly mounted on the left and right ends of the support plate (4), respectively. The other ends of the two sets of springs B (21) are fixedly mounted on the two sets of sliding blocks (18). A driving mechanism is mounted on the lower end of the support plate (4). The driving mechanism is used to provide power for shaking the support plate (4).

5. A novel Pseudomonas fluorescens fermentation shaker as claimed in claim 4, characterized in that: The driving mechanism comprises a support frame (22), a servo motor (23), a rotating shaft (24) and an eccentric block (25); the servo motor (23) and the support frame (22) are both fixedly mounted on the lower end of the support plate (4); the rotating shaft (24) is rotatably mounted on the support frame (22); the upper end of the rotating shaft (24) is connected to the output end of the servo motor (23); and the eccentric block (25) is fixedly mounted on the lower end of the rotating shaft (24).

6. A novel Pseudomonas fluorescens fermentation shaker as claimed in claim 4, characterized in that: The moving mechanism comprises a connecting plate (26), a sliding rod (27), a push rod (28) and a hydraulic cylinder (29); the connecting plate (26) is fixedly mounted on the rear ends of the two sets of fixed plates (17); the connecting plate (26) is located in the chamber of the fermentation box (2); the sliding rod (27) and the push rod (28) are both mounted on the fermentation box (2) so as to slide forward and backward; the rear end of the push rod (28) is connected to the output end of the hydraulic cylinder (29); and the hydraulic cylinder (29) is fixedly mounted on the rear end of the fermentation box (2).

7. A novel Pseudomonas fluorescens fermentation shaker as claimed in claim 1, characterized in that: The fermentation box (2) is provided with a thermometer and hygrometer.

Images