Suspension cell culture bottle capable of improving ventilation efficiency

Abstract

The utility model relates to a suspension cell culture bottle capable of improving ventilation efficiency, which is characterized by comprising a culture bin for cell culture and two ventilation holes positioned on the short side of the top. The air hole is located in the other side of the bottle opening I and the bottle opening II. The culture bottle is large in bottom area and low in height, the volume of the culture bottle is increased by increasing the bottom area of the culture bottle, meanwhile, a liquid-gas interface of a solution is increased along with the increase of the bottom area of the culture bottle, and the gas exchange efficiency of a cell culture solution is greatly improved. Except a conventional gas exchange way through a hydrophobic filter membrane at a bottle opening, the two air vents of the culture bottle provide an additional gas exchange way, so that the culture bottle can still provide enough gas exchange under the condition of culturing a large amount of cell suspension.

Description

Technical Field

[0001] The utility model relates to the field of cell culture devices, in particular to a suspension cell culture bottle with improved air permeability efficiency. Background Art

[0002] A cell culture flask is a cell culture device that consists of a single-layer structure according to the size of the culture area. It can achieve various scales of cell culture and is suitable for many fields such as vaccines, pharmaceutical industry or monoclonal antibodies. There are many specifications of single-layer cell culture flasks on the market, such as T25 cell culture flasks, T175 cell culture flasks, etc., which are widely used in scientific research and various scales of production. According to whether the culture surface is treated or not, it can be divided into cell culture flasks for adherent culture and suspension culture, which are favored by many scientific research institutions and pharmaceutical companies.

[0003] However, the bottom surface of the cell culture flask for suspension culture on the current market is very close to the bottle mouth at the side of the culture flask, which cannot meet the needs of large-volume suspension culture cells. Currently, highly breathable cell culture bags are used in the market for suspension cell culture. However, this product still has defects such as insufficient air permeability and easy contamination. The new cell culture flask improves gas exchange efficiency by increasing air holes and increasing the bottom area of ​​the culture flask, providing users with another suspension cell culture solution. Utility Model Content

[0004] The utility model aims to overcome the above defects of the existing cell culture bags, improve the existing cell culture bottles, and provide a suspension cell culture bottle with improved air permeability efficiency.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the utility model is: a suspension cell culture bottle with improved ventilation efficiency, characterized in that it includes a culture chamber for cell culture and two ventilation holes located on the short sides of the top.

[0006] Furthermore, the culture chamber is composed of bottle mouth I, bottle mouth II and a cell culture chamber. Compared with common cell culture bottles, the culture bottle has two bottle mouths located at the top of the culture bottle instead of the side.

[0007] Furthermore, the two air holes located on the short side of the top are sealed with a 0.22 μm hydrophobic filter membrane to increase the gas exchange pathway of the culture bottle. The air holes are located on the other side of the bottle mouth I and bottle mouth II.

[0008] Furthermore, the culture bottle has a large bottom area and a low height, and the volume of the culture bottle is increased by increasing the bottom area of ​​the culture bottle, and at the same time, the liquid-gas interface of the solution increases with the increase of the bottom area of ​​the culture bottle, thereby improving the gas exchange efficiency of the cell culture solution. In addition to the conventional gas exchange pathway through the hydrophobic filter membrane of the bottle mouth, the two air holes of the culture bottle provide an additional gas exchange pathway, ensuring that the culture bottle can still provide sufficient gas exchange when a large number of cell suspensions are cultured.

[0009] Compared with the prior art, the utility model has the following beneficial effects: the traditional cell culture bottle has only one bottle mouth and does not have the two additional vent holes added by the utility model. The cell culture bottle for suspension cell culture of the utility model provides a highly breathable and large-capacity cell culture bottle for existing suspension cell culture. The culture bottle has good air permeability and, compared with the common cell culture bags on the market, has better sealing performance and is not prone to contamination, which can provide users with more choices when conducting suspension cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 The figure is a schematic diagram of the structure of a suspension cell culture bottle with improved gas permeability efficiency. DETAILED DESCRIPTION

[0011] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model will be described in detail below.

[0012] like Figure 1 As shown, it includes a culture chamber for cell culture and two air holes located on the short side of the top; the culture chamber is composed of bottle mouth I (1), bottle mouth II (2) and cell culture chamber (3). Compared with common cell culture bottles, this culture bottle has two bottle mouths and is located on the top of the culture bottle instead of the side.

[0013] Furthermore, the two air holes (4) located on the short side of the top are sealed with a 0.22 μm hydrophobic filter membrane to increase the gas exchange pathway of the culture bottle. The air holes are located on the other side of the bottle mouth I (1) and the bottle mouth II (2).

[0014] Furthermore, the culture bottle has a large bottom area and a low height, and the volume of the culture bottle is increased by increasing the bottom area of ​​the culture bottle, and at the same time, the liquid-gas interface of the solution increases with the increase of the bottom area of ​​the culture bottle, which greatly improves the gas exchange efficiency of the cell culture solution. In addition to the conventional gas exchange path through the hydrophobic filter membrane of the bottle mouth, the two air holes of the culture bottle provide an additional gas exchange path to ensure that the culture bottle can still provide sufficient gas exchange when a large number of cell suspensions are cultured.

[0015] It is not difficult to find from the above embodiments that the cell culture bottle can provide a highly air-permeable, large-capacity cell culture bottle for existing suspension cell culture. The culture bottle has good air permeability and, compared with common cell culture bags on the market, has better sealing performance and is not prone to contamination, which can provide users with more choices when conducting suspension cell culture.

[0016] The above contents are further detailed descriptions of the present invention in combination with specific preferred implementations, and the specific implementation of the present invention cannot be considered to be limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as falling within the protection scope of the present invention.

Claims

1. A suspension cell culture flask with improved air permeability efficiency, characterized in that: It includes a culture chamber for cell culture and two air holes located on the short side of the top.

2. A suspension cell culture flask with improved air permeability efficiency according to claim 1, characterized in that: The culture chamber is composed of a bottle mouth I (1), a bottle mouth II (2), a cell culture chamber (3) and two air holes (4). Compared with a common cell culture bottle, the culture bottle has two bottle mouths located at the top of the culture bottle rather than the side.

3. A suspension cell culture flask with improved air permeability efficiency according to claim 2, characterized in that: The two air holes (4) located on the short sides of the top are sealed with a 0.22 μm hydrophobic filter membrane, and the air holes (4) are located on the other side of the bottle mouth I (1) and the bottle mouth II (2).

4. The suspension cell culture flask with improved air permeability efficiency according to claim 1, characterized in that: The culture bottle has a large bottom area and a low height. The volume of the culture bottle is increased by increasing the bottom area of ​​the culture bottle. At the same time, the liquid-gas interface of the solution increases as the bottom area of ​​the culture bottle increases, thereby improving the gas exchange efficiency of the cell culture solution. In addition to the conventional gas exchange path through the hydrophobic filter membrane of the bottle mouth, the two air holes of the culture bottle provide additional gas exchange paths, thereby ensuring that the culture bottle can still provide sufficient gas exchange when a large number of cell suspensions are cultured.

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