Nucleic acid protein purification device

Abstract

The present disclosure relates to a nucleic acid protein purification device comprising: a plurality of filter plates arranged along an X direction, the filter plates having a plurality of filter holes in an array, filter membranes being arranged in the filter holes; a vacuum generator, a vacuum generating port of the vacuum generator being connected to the filter holes so as to generate negative pressure in the filter holes; and a liquid separation mechanism, the liquid separation mechanism comprising: a liquid separation head located above the filter plates; and a displacement assembly, the liquid separation head being arranged on the displacement assembly, the displacement assembly driving the liquid separation head to move along the X direction so that the liquid separation head can pass through the plurality of the filter plates.

Description

Technical Field

[0001] The present disclosure relates to the technical field of nucleic acid detection, in particular to a nucleic acid protein purification device. Background Art

[0002] Nucleic acid and protein purification is usually carried out in a filter plate with multiple filter holes. A filter membrane is usually provided in the filter holes. When a pressure difference is formed between the top and bottom of the filter holes, the nucleic acid sample in the filter holes will flow downward. Under the action of the filter membrane, the required protein remains in the filter holes, and other impurities pass through the filter membrane.

[0003] Currently, nucleic acid samples need to be manually added to multiple filter holes, and only one filter plate can be filtered during filtration, resulting in low nucleic acid protein purification efficiency. Utility Model Content

[0004] The purpose of the present invention is to provide a nucleic acid protein purification device to improve the nucleic acid protein purification efficiency.

[0005] To achieve this object, the nucleic acid protein purification device provided by the present disclosure includes: a plurality of filter plates arranged along the X direction, the filter plates having a plurality of filter holes in an array, and filter membranes are arranged in the filter holes; a vacuum generator, the vacuum generating port of the vacuum generator is connected to the filter holes so as to generate negative pressure in the filter holes; and a liquid separation mechanism, the liquid separation mechanism includes: a liquid separation head located above the filter plates; and a displacement assembly, the liquid separation head is arranged on the displacement assembly, and the displacement assembly drives the liquid separation head to move along the X direction so that the liquid separation head can pass through the plurality of the filter plates.

[0006] According to the nucleic acid protein purification device disclosed in the present invention, the displacement component can drive the liquid separation head to pass through multiple filter plates, thereby adding nucleic acid samples to the multiple filter plates. In addition, the vacuum generator can evacuate each filter plate, so that the nucleic acid samples in the multiple filter plates are filtered at the same time. As a result, the nucleic acid protein purification device disclosed in the present invention improves the efficiency of nucleic acid protein purification. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] Figure 1 A schematic diagram of a nucleic acid protein purification device provided in an embodiment of the present disclosure is shown.

[0008] Figure 2 An exploded view of a workbench and a filter plate provided by an embodiment of the present disclosure is shown.

[0009] Figure 3 A schematic diagram of the bottom of the filter plate provided in an embodiment of the present disclosure is shown.

[0010] Figure 4A cross-sectional view of the filter plate provided by an embodiment of the present disclosure when installed on a workbench is shown.

[0011] Reference numerals:

[0012] 1. Bottom plate; 2. Shell; 21. Opening; 3. Filter plate; 31. Filter hole; 311. First section; 312. Second section; 313. Step surface; 4. Liquid separation mechanism; 41. Liquid separation head; 411. First liquid separation channel group; 412. Second liquid separation channel group; 5. Workbench; 51. Table; 52. Limiting plate; 521. Through hole; 53. Mounting groove; 531. Connecting port; 54. Annular protrusion; 55. Receiving groove; 56. Waste liquid groove; 561. Waste liquid discharge port. DETAILED DESCRIPTION

[0013] Figure 1 A schematic diagram of a nucleic acid protein purification device provided by an embodiment of the present disclosure is shown. Figure 1 As shown, the nucleic acid protein purification device includes a bottom plate 1 and a shell 2 located on one side of the bottom plate 1. A plurality of filter plates 3 arranged along the X direction are arranged on the bottom plate 1, and the filter plates 3 have a plurality of filter holes 31 in an array. A filter membrane (not shown) is arranged in the filter hole 31, and the filter membrane is, for example, a silicon matrix membrane. The vacuum generating port of the vacuum generator (not shown) is connected to the filter hole 31 so as to generate negative pressure in the filter hole 31. A liquid separation mechanism 4 is also arranged on the bottom plate 1, and the liquid separation mechanism 4 includes a liquid separation head 41 located above the filter plate 3 and a displacement assembly (not shown) located inside the shell 2, and the displacement assembly can be a screw assembly, a belt assembly, etc. An opening 21 for the liquid separation head 41 to enter the inside of the shell 2 is arranged on the shell 2, and the opening 21 extends along the X direction. The displacement assembly drives the liquid separation head 41 to move along the X direction so that the liquid separation head 41 can pass through the plurality of filter plates 3, and the liquid separation head 41 is configured to inject nucleic acid samples into the filter holes.

[0014] According to the nucleic acid protein purification device disclosed in the present invention, the displacement component can drive the liquid separation head 41 to pass through multiple filter plates 3, thereby adding nucleic acid samples to the multiple filter plates 3. In addition, the vacuum generator can evacuate each filter plate 3, so that the nucleic acid samples in the multiple filter plates 3 are filtered simultaneously. Therefore, the nucleic acid protein purification device disclosed in the present invention improves the nucleic acid protein purification efficiency.

[0015] like Figure 1 As shown, the plurality of filter holes 31 on each filter plate 3 are arranged along the X direction and the Y direction to form an array, wherein the Y direction is perpendicular to the X direction.

[0016] like Figure 1As shown, the liquid dispensing head 41 has a first liquid dispensing channel group 411, and the first liquid dispensing channel group 411 includes a plurality of liquid dispensing channels arranged along the Y direction. Thus, the liquid dispensing head 41 can add nucleic acid samples to a plurality of filter holes 31 on the filter plate 3 at the same time.

[0017] like Figure 1 As shown, the liquid dispensing head 41 also has at least one second liquid dispensing channel group 412, and the second liquid dispensing channel group 412 and the first liquid dispensing channel group 411 are arranged along the X direction. Figure 1 In the embodiment, there are three second liquid separation channel groups 412 , but in other embodiments, the number of second liquid separation channel groups 412 can be adjusted as needed.

[0018] Figure 2 Schematic diagram of the liquid dispensing head 41, the workbench 5 and the filter plate 3 provided in the embodiment of the present disclosure is shown. Figure 1 and Figure 2 As shown, the nucleic acid protein purification device also includes a workbench 5 installed on the bottom plate 1, the workbench 5 includes a table body 51 and a limiting plate 52 installed on the top of the table body 51, the limiting plate 52 has a plurality of through holes 521, and the shape of the through holes 521 is slightly larger than the shape of the filter plate 3, so that when the limiting plate 52 is installed on the table body 51, a plurality of mounting grooves 53 arranged along the X direction are formed on the workbench 5, the filter plate 3 is arranged in the mounting groove 53, and the vacuum generating port of the vacuum generator is connected to the mounting groove 53.

[0019] like Figure 2 As shown, the bottom of the mounting groove 53 has a connecting port 531, and the vacuum generating port is connected to the connecting port to generate negative pressure in the mounting groove 53, so that the pressure at the bottom of the filter hole 31 is less than the pressure at the top of the filter hole 31, so that the nucleic acid sample in the filter hole 31 can flow downward.

[0020] like Figure 2 As shown, the mounting groove 53 includes an annular protrusion 54 and a receiving groove 55 which is located inside the annular protrusion 54 and is recessed downward, and the connecting port is located in the receiving groove 55. When the filter plate 3 is installed on the mounting groove 53, the annular protrusion 54 supports the filter plate 3, and the bottom of the filter hole 31 is located in and extends downward into the receiving groove 55.

[0021] Figure 3 A schematic diagram of the bottom of the filter plate 3 provided in an embodiment of the present disclosure is shown. Figure 4 FIG. 2 shows a cross-sectional view of the filter plate 3 provided in the embodiment of the present disclosure when it is installed on the workbench 5. Figure 3 and Figure 4As shown, the filter hole 31 includes a first section 311 and a second section 312, wherein the first section 311 is close to the top of the filter hole 31, and the second section 312 is close to the bottom of the filter hole 31. The diameter of the first section 311 is greater than the diameter of the second section 312, so a step surface 313 is formed at the connection between the first section 311 and the second section 312. In addition, the second section 312 extends to a position below the bottom of the filter plate 3, so that the second section 312 protrudes downward from the bottom of the filter plate 3. When the filter plate 3 is installed on the mounting groove 53, the step surface 313 abuts against the annular protrusion 54, and the second section 312 is located in the accommodating groove 55.

[0022] like Figure 1 As shown, the workbench 5 further comprises a waste liquid tank 56 , and the waste liquid tank 56 and the mounting tank 53 are arranged along the X direction. The bottom of the waste liquid tank 56 comprises a waste liquid discharge port 561 .

[0023] The above is a description of the embodiments of the present disclosure. Through the above description of the disclosed embodiments, professionals and technicians in the field can implement or use the present disclosure. Various modifications to these embodiments will be apparent to professionals and technicians in the field. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present disclosure. Therefore, the present disclosure will not be limited to the embodiments shown herein, but will conform to the widest range consistent with the principles and novelties disclosed herein.

Claims

1. A nucleic acid and protein purification device, characterized in that: The nucleic acid protein purification device comprises: A base plate (1), and a workbench (5) mounted on the base plate (1); A plurality of filter plates (3) arranged along the X direction, the filter plates (3) having a plurality of filter holes (31) in an array, and filter membranes being arranged in the filter holes (31); a vacuum generator, wherein a vacuum generating port of the vacuum generator is in communication with the filter hole (31) so as to generate negative pressure in the filter hole (31); and A liquid separation mechanism (4), wherein the liquid separation mechanism (4) comprises: a liquid dispensing head (41) located above the filter plate (3); and A displacement component, the liquid separation head (41) is arranged on the displacement component, and the displacement component drives the liquid separation head (41) to move along the X direction so that the liquid separation head (41) can pass through a plurality of filter plates (3).

2. The nucleic acid protein purification device according to claim 1, characterized in that: The plurality of filter holes (31) are arranged along the X direction and the Y direction to form the array, wherein the Y direction is perpendicular to the X direction.

3. The nucleic acid protein purification device according to claim 2, characterized in that: The liquid dispensing head (41) has a first liquid dispensing channel group (411), and the first liquid dispensing channel group (411) comprises a plurality of liquid dispensing channels arranged along the Y direction.

4. The nucleic acid protein purification device according to claim 3, characterized in that: The liquid dispensing head (41) further comprises at least one second liquid dispensing channel group (412), wherein the second liquid dispensing channel group (412) and the first liquid dispensing channel group (411) are arranged along the X direction.

5. The nucleic acid protein purification device according to claim 1, characterized in that: The nucleic acid protein purification device also includes: A plurality of mounting grooves (53) are arranged along the X direction, the filter plate (3) is arranged in the mounting groove (53), and the vacuum generating port of the vacuum generator is in communication with the mounting groove (53).

6. The nucleic acid protein purification device according to claim 5, characterized in that: The bottom of the installation groove (53) is provided with a connection port (531), and the vacuum generating port is in communication with the connection port (531).

7. The nucleic acid protein purification device according to claim 6, characterized in that: The mounting groove (53) comprises an annular protrusion (54) and a receiving groove (55) located inside the annular protrusion (54) and recessed downwards, the connecting port being located inside the receiving groove (55), and when the filter plate (3) is mounted on the mounting groove (53), the annular protrusion (54) supports the filter plate (3), and the bottom of the filter hole (31) is located inside and extends downwards into the receiving groove (55).

8. The nucleic acid protein purification device according to claim 7, characterized in that: The filter hole (31) comprises a first section (311) located at the upper part and a second section (312) located at the lower part, and a step surface (313) is formed at the connection between the first section (311) and the second section (312). When the filter plate (3) is installed on the installation groove (53), the step surface (313) abuts against the annular protrusion (54).

9. The nucleic acid protein purification device according to claim 5, characterized in that: The workbench (5) further comprises a waste liquid tank (56), wherein the waste liquid tank (56) and the mounting tank (53) are arranged along the X direction.

10. The nucleic acid protein purification device according to claim 9, characterized in that: The bottom of the waste liquid tank (56) is provided with a waste liquid discharge port (561).

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