A dark field illumination module and an integrated dark field microscope
By designing a dark field illumination module including a light source fixed ring seat, a plastic lens array and a deflection prism in a dark field microscope, the problem of cumbersome adjustment of dark field light sources when adjusting the focal length of the dark field microscope is solved, and simple formation and rapid adjustment of the dark field are achieved.
Patent Information
- Application Number
- CN202420429401.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-03-05
- Publication Date
- 2025-04-01
- Estimated Expiration
- 2034-03-05
AI Technical Summary
When adjusting the focal length of the microscope, existing dark field microscopes need to adjust the optical system of the dark field light source, which makes the adjustment process cumbersome and difficult.
A dark field lighting module is designed, including a light source fixed ring seat, a plastic lens array and a deflection prism, through which the flattened beam is formed, simplifying the formation and adjustment of the dark field beam.
This module makes the formation of dark field beams simpler and more convenient, ensures the formation of dark field of view, and can quickly replace the deflection prism when adjusting the focal length to adapt to changes in working distance.
Smart Images
Figure CN222704810U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of dark field microscopy, in particular to a dark field illumination module and an integrated dark field microscope. Background Art
[0002] Dark-field microscopy uses the principle of the Tyndall optical effect. By changing the direction of the field light source, most of the light except the scattered light of the sample to be tested cannot enter the microscope objective, making the background of the microscope's observation field a dark field, while the light diffracted back by the sample and the scattered light are brightly visible in the dark field.
[0003] In order to reduce the size of a dark field microscope, some dark field microscopes integrate a dark field light source and its optical system with a microscope objective. When the focal length of the microscope objective is adjusted, the working distance of the dark field microscope will change. At this time, the optical system of the dark field light source needs to be adjusted to change the working distance of the light source. However, the dark field light source and its optical system of existing dark field microscopes are difficult and cumbersome to adjust. Utility Model Content
[0004] Based on this, the purpose of the utility model is to provide a dark field illumination module and an integrated dark field microscope, which have the advantages of simple structure and easy replacement of deflection prisms.
[0005] A dark field lighting module comprises a light source fixing ring seat, a shaping lens array and a deflection prism; the light source fixing ring seat, the shaping lens array and the deflection prism are coaxially arranged in sequence, the light source fixing ring seat is provided with a plurality of light outlets, the shaping lens array covers the light outlets and is used for shaping the light beams emitted from the light outlets into downward parallel light beams; the deflection prism is arranged below the shaping lens array and is used for deflecting the shaped parallel light beams.
[0006] The dark field illumination module of the utility model is easy to replace the fixed deflection prism, so as to ensure that the dark field light beam forms a flat-top light beam.
[0007] Furthermore, a plurality of the light outlets are arranged in a surrounding manner below the light source fixing ring seat, and the shaping lens array is arranged below the light source fixing ring seat cover.
[0008] Furthermore, it also includes a fixing ring sleeve, which is arranged below the light source fixing ring seat, and the deflection prism is arranged in the fixing ring sleeve.
[0009] Furthermore, the shaping lens array includes a plurality of shaping lenses and a lens ring seat, wherein the lens ring seat is surrounded by a plurality of lens grooves, and the shaping lenses are arranged in the lens grooves; the lens ring seat is detachably arranged below the light source fixing ring seat, and the shaping lenses are directly opposite to the light outlet.
[0010] Furthermore, the shaping lens array includes a plurality of shaping lenses and a lens ring seat, the lens ring seat is surrounded by a plurality of lens grooves, and the shaping lenses are arranged in the lens grooves; a lens ring seat groove corresponding to the lens ring seat is provided in the fixing ring sleeve, and the lens ring seat is clamped between the fixing ring sleeve and the light source fixing ring seat through the lens ring seat groove.
[0011] Furthermore, the shaping lens array also includes a filter plate, which is arranged on the lens ring seat; the filter plate is provided with a plurality of filter holes, and the filter holes correspond to the lens grooves one by one.
[0012] Furthermore, the deflection prism is annular in shape, and a protrusion is provided around the outside of the top of the deflection prism. The inner diameter of the protrusion is larger than the distance from the outside of the shaping lens to the axis of the shaping lens array. The protrusion surrounds and covers the shaping lens and abuts against the lens ring seat.
[0013] Furthermore, the vertex angle of the deflection prism is 40° to 80°.
[0014] Furthermore, it also includes an objective lens seat, which is sleeved with the microscope objective lens and is arranged on the light source fixing ring seat; the microscope objective lens is connected to the light source fixing ring seat through the objective lens seat.
[0015] Furthermore, a dark field light source is provided in the light source fixing ring seat, and the dark field light source is an illumination optical fiber, and a plurality of light emitting points are provided on the illumination optical fiber, and the light emitting points correspond one-to-one to the light outlets.
[0016] An integrated dark field microscope comprises the above-mentioned dark field illumination module; the microscope objective lens is coaxially arranged with the dark field illumination module.
[0017] In order to better understand and implement the present invention, the present invention is described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 A three-dimensional schematic diagram of the integrated dark-field microscope of the present application;
[0019] Figure 2 This is an assembly diagram of the parts of the integrated dark field microscope of this application;
[0020] Figure 3 A cross-sectional front view of the integrated dark field microscope of the present application;
[0021] Figure 4 It is a schematic diagram of the illumination distribution simulation of a dark field microscope when the vertex angle of the deflection prism of the present application is 60°;
[0022] Figure 5This is a three-dimensional schematic diagram of the shaping lens array of the present application. DETAILED DESCRIPTION
[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0024] In the description of the present invention, it should be noted that the terms "vertical direction", "upper", "lower", "horizontal" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as limiting the present invention. In addition, "first", "second", "third", and "fourth" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.
[0025] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a heat-conducting connection, a detachable connection, or an integral connection, a mechanical connection, an electrical connection, a direct connection, or a connection through an intermediate medium, or the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0026] Example
[0027] See also Figures 1 to 3 , an embodiment of the present application provides an integrated dark field microscope. The integrated dark field microscope includes a microscope objective 1 and a dark field illumination module; the dark field illumination module includes a light source fixing ring seat 2, a shaping lens array 3, a deflection prism 4 and a fixing ring sleeve 5. The microscope objective 1, the light source fixing ring seat 2, the deflection prism 4 and the fixing ring sleeve 5 are coaxially arranged in sequence, a plurality of light outlets 2a are arranged around the bottom of the light source fixing ring seat 2, the shaping lens array 3 covers the light outlets 2a, the fixing ring sleeve 5 is sleeved under the light source fixing ring seat 2, and the deflection prism 4 is arranged between the shaping lens array 3 and the fixing ring sleeve 5.
[0028] Furthermore, a dark-field light source is provided in the light source fixing ring seat 2. The dark-field light beam emitted by the dark-field light source is emitted through the light outlet 2a, and forms parallel light after passing through the shaping lens array 3; the parallel light is deflected by the deflection prism 4, and then irradiates the sample at an inclined angle; since the light irradiates the sample at an inclined angle, the background and the direct reflected light of the sample cannot enter the microscope objective 1, and only the scattered light can enter the microscope objective 1, forming dark-field illumination. In some embodiments, the dark-field light source is an illumination optical fiber, and a plurality of light-emitting points are provided on the illumination optical fiber, and the light-emitting points correspond to the light outlets one by one. The illumination optical fiber can provide light-emitting points with the same light-emitting characteristics for multiple light outlets 2a, which is conducive to ensuring the uniformity of the overall light source. In other embodiments, the dark-field light source is an LED array arranged in a surrounding manner.
[0029] The dark field beam provided by the illumination fiber is shaped and deflected by the shaping lens array 3 and the deflection prism 4, and then irradiated on the sample. Within a certain working distance, a flattened beam is formed. The flattened beam can provide uniform illumination in a certain area to prevent the brightness of the illumination in the middle of the sample from being too high when using the dark field microscope, which affects the observation. When the microscope adjusts its focal length, the working distance of the microscope will also change, causing the flattened beam to become a Gaussian beam; at this time, the deflection prism 4 needs to be replaced to change the inclination angle of the beam after passing through the deflection prism 4. Please refer to Figure 4 When the vertex angle of the deflection prism 4 is 60°, the optimal working distance for the dark field beam to form a flattened beam is 10-14 mm. Further, when the vertex angle of the deflection prism 4 is 80°, the optimal working distance for the dark field beam to form a flattened beam is 0-2 mm; when the vertex angle of the deflection prism 4 is 40°, the optimal working distance for the dark field beam to form a flattened beam is 25-35 mm. As the vertex angle of the deflection prism 4 decreases, the working distance for the dark field beam to form a flattened beam increases, and the working range for forming a flattened beam increases.
[0030] See also Figure 5 In some embodiments, the shaping lens array 3 includes a plurality of shaping lenses 31 and a lens ring seat 32. The lens ring seat 32 is surrounded by a plurality of lens grooves, and the shaping lenses 31 are arranged in the lens grooves. Furthermore, the shaping lens array 3 also includes a filter plate 33, and the filter plate 33 is arranged on the lens ring seat 32; the filter plate 33 is provided with a plurality of filter holes, and the filter holes correspond to the lens grooves one by one. Through the filter holes, stray light whose propagation direction deviates greatly from the optical axis of the shaping lens 31 can be filtered out before the light enters the shaping lens 31. In some embodiments, the filter plate 33 and the lens ring seat 32 are integrally formed.
[0031] In some embodiments, the lens ring seat 32 is detachably disposed below the light source fixed ring seat 2, and the shaping lens 31 is directly opposite to the light outlet 2a. In other embodiments, a lens ring seat groove corresponding to the lens ring seat 32 is disposed in the fixed ring sleeve 5, and the lens ring seat 32 is clamped between the fixed ring sleeve 5 and the light source fixed ring seat 2 through the lens ring seat groove. By disassembling the lens ring seat 32, the shaping lens array 3 can be conveniently replaced, and by changing the angle between the optical axis of the shaping lens 31 and the axis of the lens ring seat 32, the irradiation range of the dark field light beam can be changed.
[0032] Furthermore, the deflection prism 4 is annular, and when installed, the deflection prism 4 is placed in the fixed ring sleeve 5. In some embodiments, a protrusion 4a is provided around the outer side of the top of the deflection prism 4. Further, the inner diameter of the protrusion 4a is greater than the distance from the outer side of the shaping lens 31 to the axis of the shaping lens array 3. When the deflection prism 4 is arranged below the light source fixed ring seat 2, the protrusion 4a surrounds and covers the shaping lens 31, and abuts against the lens ring seat 32 to reduce the dark field light beam overflow. In some embodiments, a receiving groove that matches the protrusion 4a is provided in the fixed ring sleeve 5. The deflection prism 4 is placed in the fixed ring sleeve 5 and fixed by the protrusion 4a; the deflection prism 4 is arranged below the light source fixed ring seat 2 through the fixed ring sleeve 5, facing the shaping lens array 3.
[0033] Furthermore, the dark field illumination module of the embodiment of the present application further comprises an objective lens holder 1a, which is sleeved with a microscope objective lens 1 and is disposed on a light source fixing ring holder 2; the microscope objective lens 1 is connected to the light source fixing ring holder 2 via the objective lens holder 1a.
[0034] Compared with the prior art, the dark field illumination module of the embodiment of the present application can easily replace the deflection prism 4 and the shaping lens array 3. When the working distance changes significantly due to the adjustment of the focal length of the microscope objective 1 and the deflection prism 4 needs to be replaced, it is only necessary to remove the fixing ring sleeve 5, take out the deflection prism 4 from the fixing ring sleeve 5 and replace it. The dark field illumination module of the embodiment of the present application is easy to load and unload, simple to operate, and improves the operating efficiency.
[0035] The above-mentioned embodiments only express several implementation methods of the utility model, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the utility model patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, and the utility model is also intended to include these modifications and modifications.
Claims
1. A dark field lighting module, characterized in that: It includes a light source fixing ring seat, a shaping lens array and a deflection prism; the light source fixing ring seat, the shaping lens array and the deflection prism are coaxially arranged in sequence, the light source fixing ring seat is provided with a plurality of light outlets, the shaping lens array covers the light outlets and is used for shaping the light beams emitted from the light outlets into downward parallel light beams; the deflection prism is arranged below the shaping lens array and is used for deflecting the shaped parallel light beams.
2. The dark field lighting module according to claim 1, characterized in that: A plurality of light outlets are arranged around and below the light source fixing ring seat, and the shaping lens array is arranged below the light source fixing ring seat cover.
3. The dark field lighting module according to claim 2, characterized in that: It also includes a fixing ring sleeve, which is sleeved below the light source fixing ring seat, and the deflection prism is arranged in the fixing ring sleeve.
4. The dark field lighting module according to claim 3, characterized in that: The shaping lens array comprises a plurality of shaping lenses and a lens ring seat, wherein the lens ring seat is surrounded by a plurality of lens grooves, and the shaping lenses are arranged in the lens grooves; the lens ring seat is detachably arranged below the light source fixing ring seat, and the shaping lenses face the light outlet.
5. The dark field lighting module according to claim 3, characterized in that: The shaping lens array includes a plurality of shaping lenses and a lens ring seat. The lens ring seat is surrounded by a plurality of lens grooves, and the shaping lenses are arranged in the lens grooves. The fixed ring sleeve is provided with a lens ring seat groove corresponding to the lens ring seat, and the lens ring seat is clamped between the fixed ring sleeve and the light source fixed ring seat through the lens ring seat groove.
6. The dark field lighting module according to any one of claims 4-5, characterized in that: The shaping lens array also includes a filter plate, which is arranged on the lens ring seat; the filter plate is provided with a plurality of filter holes, which correspond to the lens grooves one by one.
7. The dark field lighting module according to claim 6, characterized in that: The deflection prism is annular in shape, and a protrusion is provided around the outside of the top of the deflection prism. The inner diameter of the protrusion is larger than the distance from the outside of the shaping lens to the axis of the shaping lens array. The protrusion surrounds and covers the shaping lens and abuts against the lens ring seat.
8. The dark field lighting module according to claim 7, characterized in that: The vertex angle of the deflection prism is 40° to 80°.
9. The dark field lighting module according to claim 8, characterized in that: A dark field light source is arranged in the light source fixing ring seat. The dark field light source is an illumination optical fiber. A plurality of light emitting points are arranged on the illumination optical fiber. The light emitting points correspond to the light outlets one by one.
10. An integrated dark field microscope, characterized in that: include: A microscope objective lens and a dark field illumination module as described in any one of claims 1 to 9; The microscope objective lens is coaxially arranged with the dark field illumination module.
11. The integrated dark field microscope according to claim 10, characterized in that: It also includes an objective lens seat, which is sleeved with the microscope objective lens and is arranged on the light source fixing ring seat; the microscope objective lens is connected to the light source fixing ring seat through the objective lens seat.