Egg collector

By designing an egg collector including egg laying barrels and odor source buckets, using attractants and light sources to lure pests to lay eggs, the problem of difficulty in obtaining test insects in resistance monitoring is solved, and work efficiency and timeliness are improved.

CN222707375UActive Publication Date: 2025-04-04ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Patent Information

Application Number
CN202421358488.0
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Priority Date
2023-06-14
Filing Date
2024-06-14
Publication Date
2025-04-04
Estimated Expiration
2034-06-14

AI Technical Summary

Technical Problem

In the prior art, test insects are difficult to obtain and inefficient in pest resistance monitoring, resulting in low efficiency and timeliness of resistance monitoring.

Method used

A brand new insect egg collector is designed, including egg-laying buckets and flavour barrels, which can lure insects into the egg-laying buckets through attractants and light sources in the flavour barrels, and obtain experimental insects through daily collection of egg blocks.

Benefits of technology

It significantly improves the working efficiency and timeliness of resistance monitoring, shortens the time to obtain experimental pests, thus providing a more convenient and effective sample for pest resistance research.

✦ Generated by Eureka AI based on patent content.

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Patent Text Reader

Abstract

The utility model provides a worm egg collector which comprises a worm egg collecting barrel and a smell source barrel which are of a detachable integrated structure and used for completing automatic trapping of insects and automatic collection of worm eggs. The insect egg collecting barrel is detachably fixed above the smell source barrel, the insect egg collecting barrel and the smell source barrel are located on the same connecting line, the connecting line is perpendicular to the horizontal plane, and by arranging the structures such as the insect egg collecting barrel and the smell source barrel and cooperating with a matched attracting method, the attracting effect on insects and the insect egg collecting efficiency are remarkably improved; according to the insect trapping device, insect gathering is better promoted, insects are induced to lay eggs, eggs are efficiently collected, meanwhile, target insects can be attracted and collected more accurately, and the insect trapping device has important significance and positive promotion effect on entomology research, pest control and development of related ecological fields; meanwhile, a solid theoretical basis and a technical means are provided for subsequent research on drug resistance of insects.
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Description

Technical Field

[0001] The utility model belongs to the technical field of pest control, and in particular relates to an insect egg collector. Background Art

[0002] The description of the background technology is merely a general description for facilitating understanding of the content of the present invention and does not constitute any limitation to the present invention.

[0003] Chemical pesticides have become an indispensable means of production for modern agriculture, and the unscientific and irrational use of pesticides will lead to the outbreak of insect resistance, seriously affecting the safety of food and cash crops. Insect resistance is a potential, powerful and common natural phenomenon, and human activities largely affect and control the speed and severity of insect resistance. Since the first discovery of the resistance of American pear scale (Aspidiotus pemiciosus) to lime sulfur in 1908, more than 600 species of pests and mites have been reported to have developed resistance, and the time required for resistance is getting shorter and shorter. With the increase in the types of pesticides and their widespread use, the number of insect species with resistance is increasing, so it is urgent to carry out resistance monitoring and resistance management.

[0004] The premise of pest resistance management is to clarify the resistance level of target pests. Therefore, insect resistance research first needs to establish a set of accurate and easy-to-operate resistance monitoring and detection methods, correctly understand the extent of resistance, and further study its resistance mechanism. Through pest resistance monitoring, the resistance level and its distribution can be measured in a timely and accurate manner, the types and varieties of key protected pesticides can be identified, the control effect of the entire control plan can be evaluated, and a basis for the revision of the resistance control plan can be provided. Given that many current resistance countermeasures rely on early detection of resistance, the research and development of resistance monitoring and detection technology is particularly important. With the diversification of resistance monitoring and detection purposes, resistance monitoring means and methods are also developing in a diversified direction, such as biological detection methods, biochemical detection methods, and molecular biological detection methods. However, the most commonly used and most direct resistance monitoring method is still the sensitivity-based bioassay method. At present, there are four standard bioassay methods for insecticide resistance: immersion method, film residue method or surface contact method, drip method and feeding method. The choice of method for determination should be determined according to the action characteristics and physical and chemical properties of the pesticide, the characteristics of the pest damage and other biological characteristics, and appropriate improvements can be made when necessary. However, no matter which bioassay method is used, the premise is that there are a large number of field test insects (F0 generation) collected from different regions or insects raised for one generation (F1 generation).

[0005] Directly collecting insects in the field for monitoring resistance is the most accurate biological testing method. However, since farmers carry out pest control on a regular basis, it is very difficult to collect sufficient numbers of test insects of uniform age and size (body length and weight). Therefore, when actually carrying out resistance monitoring work, larvae and pupae of various ages of target pests are mostly collected in the field, and brought back to the laboratory for further breeding using host crops or artificial feed to obtain a large number of standard test insects of uniform age and size. This cycle generally takes at least 5-7 weeks.

[0006] Therefore, in order to solve the above-mentioned problems, the utility model urgently needs to design and develop a new insect egg collector. Through the device, various types of pests can be successfully lured into the device to lay eggs, and the egg masses can be collected to obtain insects for testing, which significantly improves the work efficiency and timeliness of resistance monitoring. Utility Model Content

[0007] In order to solve the problems existing in the prior art, the utility model provides an insect egg collector and a matching luring method, wherein the insect egg collector comprises an egg-laying barrel and a flavor source barrel, and various pests are lured into a specific device (egg-laying barrel) to lay eggs by using different attractants (such as sweet and sour liquid, plant-based attractants) in the flavor source barrel in combination with a light source (ordinary LED lamp tube, special insecticidal lamp tube), and a sufficient number of test insects are obtained by collecting egg masses every day; and the method only takes 1.5-2.5 weeks to obtain sufficient test insects, which significantly improves the work efficiency and timeliness of resistance monitoring.

[0008] In order to achieve the above purpose, the utility model adopts the following scheme:

[0009] On the one hand, the utility model provides an automatic insect egg collection device, comprising an insect egg collection bucket and a flavor source bucket, wherein the insect egg collection bucket and the flavor source bucket are combined into a detachable integrated structure, and are used to automatically attract insects and automatically collect insect eggs;

[0010] The insect egg collection bucket is used to automatically attract insects, collect insect egg masses, and allow the insects to move freely;

[0011] The odor source barrel is used for automatically attracting insects, thereby improving the insect trapping capability in the insect egg collection barrel.

[0012] In some embodiments, the utility model provides a new insect egg collector, which significantly improves the insect luring effect and egg collection efficiency through the arrangement of an insect egg collection barrel (including a cover, an egg laying barrel, a cylindrical tube and other structures) and a flavor source barrel and other structures, and cooperates with its matching luring method (such as using sweet and sour liquid, plant-based attractants or enhancers to emit odors to attract insects, and using an insect-attracting light source of a specific intensity to attract insects and create suitable lighting conditions for laying eggs). It has the functions of better promoting insect aggregation, inducing insects to lay eggs and efficiently collecting eggs, and can more accurately attract and collect target insects. It has important significance and positive driving effect on the development of entomological research, pest control and related ecological fields; at the same time, it also provides a solid theoretical basis and technical means for subsequent insect resistance research, which is conducive to more accurately obtaining a large number of eggs of specific insects, and provides a sufficient experimental sample basis for conducting resistance research.

[0013] Furthermore, the insect egg collection barrel is detachably fixed above the flavor source barrel, and the centers of the insect egg collection barrel and the flavor source barrel are on the same connecting line, which is perpendicular to the horizontal plane.

[0014] Furthermore, the insect egg collection bucket includes a cover body and an egg-laying bucket, and a cylindrical tube is detachably fixed in the center of the egg-laying bucket; the cover body and the egg-laying bucket are combined into a detachable integrated structure, and the cover body is detachably fixed above the egg-laying bucket to form a relatively closed space with the egg-laying bucket to prevent insects from escaping.

[0015] Furthermore, the wall of the egg-laying barrel is provided with long strip insect entrance holes, which are equidistant and evenly distributed, and are used to provide an entry channel for insects, making it convenient for insects to enter the egg-laying barrel.

[0016] Furthermore, the bottom of the egg-laying barrel is provided with 10-30 small holes, which are evenly distributed and used to emit odor to attract insects, which is beneficial to the circulation of air and the discharge of excess water.

[0017] Furthermore, the small holes are evenly arranged in the area between the egg laying bucket and the cylindrical tube.

[0018] In some embodiments, 10-30 small holes with a diameter of 0.1-1 cm are distributed at the bottom of the egg-laying barrel, and the small holes are helpful for the discharge of excess water to ensure the suitability of the environment in the barrel. Such a setting not only creates good ventilation and drainage conditions for insects, but also can fully release the smell of the attractant in the flavor source barrel, and with the cooperation of the LED light source, further optimizes the insect's egg-laying environment, thereby attracting more insects to enter the egg-laying barrel to lay eggs; the size of the small holes is smaller than the size of the adult insects and the egg mass, thereby preventing the insects from entering the flavor source barrel and preventing the egg mass from falling into the flavor source barrel; and the results of subsequent screening experiments show that it is preferred to set 18 small holes at the bottom of the egg-laying barrel. When 18 small holes are set at the bottom of the egg-laying barrel, the effect of the attractant in the lower flavor source barrel can be maximized to attract pests into the egg-laying barrel; at the same time, limiting the optimal number and aperture of the small holes can also prevent insects from entering the flavor source barrel, so that more insects gather in the egg-laying barrel to lay eggs and move, thereby further improving the egg-laying efficiency of the pests.

[0019] Furthermore, the automatic insect egg collection device also includes an insect-attracting light source, which is detachably installed in the center of the cylindrical tube; the cylindrical tube divides the egg-laying barrel into an insect-attracting light source area, an insect activity area, and an insect egg-laying area; after the insects are attracted to the insect activity area, they lay eggs in the insect egg-laying area.

[0020] In some embodiments, the cylindrical barrel divides the interior of the egg-laying barrel into multiple functional areas, including an insect activity area, a light source area, and an egg-laying area. The insect activity area is the area between the egg-laying barrel and the cylindrical barrel, which provides sufficient space for insect activities, allowing them to move freely and find egg-laying locations; the light source area is the area inside the cylindrical barrel, and the insects are attracted to enter the egg-laying barrel by setting the best lighting conditions of the insect-attracting light source inside the cylindrical barrel 5; the egg-laying area is mainly the outer wall area of ​​the cylindrical barrel, and the inner wall of the cylindrical barrel is evenly coated with the best attractant of the utility model, and at the same time, with the insect-attracting light source, it fully combines the insect's egg-laying habits to provide a suitable surface and environment for insects to lay eggs, thereby facilitating insects to lay eggs, and the egg masses laid by insects are all in the egg-laying area. The partition design makes the functional division of the insect egg collector of the utility model clearer, avoids interference between different functional areas, provides a suitable activity environment for insects, and also optimizes the activity path of insects in the egg-laying barrel, improves the contact opportunity between insects and the egg-laying area, thereby increasing the egg-laying amount, and also improves the efficiency and convenience of collecting egg masses; and the partition design facilitates the subsequent classification and processing of the collected egg masses, and improves the convenience of subsequent research or application.

[0021] Furthermore, 8 rows of circular holes are arranged on the side surface of the cylindrical tube, and the circular holes are evenly and equidistantly arranged up and down, which facilitates the overflow of light from the insect-attracting light source area while preventing insects from entering, so that insects gather in large numbers in the insect activity area, which is convenient for the collection of egg masses.

[0022] In some embodiments, in subsequent screening experiments, the position, distribution, number and size of the circular holes cut from the side barrel surface of the cylindrical paper tube are further screened, and the results show that the position distribution, number and size settings can maximize the attraction effect on insects, while also ensuring sufficient light transmission to provide insects with a suitable egg-laying environment; in addition, the design can also prevent excessive light from entering the egg-laying barrel to prevent interference with insect activities or egg-laying behavior.

[0023] Furthermore, the long strip insect inlet hole is aligned with the circular hole on the side surface of the cylindrical tube, which is convenient for light overflow and insect trapping.

[0024] In some embodiments, the cylindrical tube is a cylindrical paper tube, and 8 rows of round holes are cut out on the side barrel surface of the cylindrical paper tube, which are evenly and equidistantly arranged up and down, with 8 holes in each row, and each round hole has a diameter of 1 cm, so as to prevent the entry of adult insects under the premise of facilitating the overflow of appropriate light. Among them, 4 rows of round holes need to be aligned with the long strip insect entrance holes. Such an arrangement can make the light of the insect-attracting light source in the center of the cylindrical paper tube overflow more smoothly, and facilitate the smooth entry of the lured male and female adults. The purpose of setting up the cylindrical paper tube is to provide a more suitable egg-laying environment for insects. The arrangement of aligning the cylindrical paper tube with the long strip insect entrance holes is conducive to using appropriate light to attract more insects into the egg-laying barrel, and combined with the insertion position of the LED light source, it can further enhance the luring effect.

[0025] The inside of the cylindrical tube is evenly and spacedly coated with an appropriate amount of attractant (such as 2-5mL of plant-derived attractant) and synergist, and the volume ratio of the plant-derived attractant to the synergist is 1:2. The plant-derived attractant can release a simulated plant scent that insects like, attracting insects to approach, and the synergist of the utility model can significantly enhance this attraction. When the smell of the plant-derived attractant is mixed with the synergist, the cylindrical tube can be simulated into the environmental conditions of plant leaves, better simulating the natural environment familiar to insects, thereby greatly improving the attraction to insects and promoting insects to lay eggs on the cylindrical tube, thereby facilitating the subsequent collection of egg masses. The egg masses can be collected by simply removing or replacing the cylindrical tube, significantly improving the egg-laying efficiency and egg mass collection capacity.

[0026] At the same time, the cylindrical tube in the utility model is preferably a cylindrical paper tube. After taking out the cylindrical paper tube, the paper with the egg mass can be directly cut off and placed in a culture dish for incubation. Subsequent experimental results show that the method can significantly shorten the time required for incubation. At the same time, the use of a cylindrical paper tube also has obvious advantages: compared with the commonly used tools such as egg shovels and egg scrapers, the utility model uses a cylindrical paper tube, which can directly cut off the paper with the egg mass and place it in a culture dish for incubation, thereby avoiding the damage to the egg mass caused by directly using the egg scraper to scrape the egg mass, thereby further improving the ability to collect egg masses and improving the hatching rate.

[0027] In the present invention, for ease of understanding, a cylindrical paper tube is used, but it can be understood that when there are other needs, other materials can be replaced as the cylindrical tube according to the needs, and it is not limited to the "paper tube".

[0028] Furthermore, the centers of the egg-laying barrel, the cylindrical barrel, and the flavor source barrel are on the same connecting line, and the connecting line is perpendicular to the horizontal plane; the flavor source barrel includes an attractant.

[0029] In some embodiments, the flavor source barrel is made of transparent organic glass, with a diameter of 25-30 cm and a height of 3-8 cm, and is fixed to the lower part of the egg collection barrel by a nut. An attractant is added to the flavor source barrel to effectively attract male and female Lepidoptera adults to enter the egg-laying barrel. The centers of the egg-laying barrel, the cylindrical paper tube, and the flavor source barrel are on the same connecting line, and the connecting line is perpendicular to the horizontal plane. The arrangement layout can significantly enhance the structural stability and functional coordination of the entire device (egg collector), thereby ensuring the smooth conduct of the luring and collection process.

[0030] The beneficial effects of the utility model are:

[0031] 1. The utility model provides a new insect egg collector, which is easy to operate and recyclable, effectively reduces manual operation and intervention in the process of collecting insect eggs, saves time and effort, improves the work efficiency and timeliness of resistance monitoring, and greatly shortens the time for obtaining pests for resistance tests; and the collection of a large number of field pests from different regions can provide a large number of standard test insect samples with uniform ages and sizes for subsequent pest resistance research, thereby providing a solid theoretical basis for the revision of pest resistance control plans, improving the level of pest resistance control, and has broad application prospects.

[0032] 2. The utility model provides a new insect egg collector, which provides a suitable egg-laying environment for insects: the design of the flavor source barrel and the egg-laying barrel provides a suitable egg-laying place for insects, and the characteristics of their shape, size and material are helpful to attract insects and promote egg-laying behavior; the selection of the position, distribution, number and size of the small holes cut on the side barrel surface of the cylindrical paper tube can maximize the attraction effect on insects and attract more insects into the egg-laying barrel; the setting of the insect-attracting light source provides appropriate lighting, attracts insects into the egg-laying barrel, and provides a suitable activity and egg-laying environment for insects; the space separation between the egg-laying barrel and the cylindrical paper tube makes the functional division of the insect egg collector clearer, including the insect activity area, the light source area and the egg-laying area, which is conducive to insect activity and improves the efficiency of collecting egg masses; the small holes distributed at the bottom of the egg-laying barrel are conducive to air circulation and the discharge of excess water, ensuring the suitability of the environment in the barrel.

[0033] 3. The utility model provides a new insect egg collector, which significantly improves the insect luring effect and egg collection efficiency through the arrangement of an insect egg collection barrel (including a cover, an egg laying barrel, a cylindrical barrel and other structures) and a flavor source barrel and other structures, and cooperates with its matching luring method (such as using sweet and sour liquid, plant-based attractants or synergists to emit odors to attract insects, and using a specific intensity of insect-attracting light sources to attract insects and create suitable lighting conditions for egg laying), has the functions of better promoting insect aggregation, inducing insect egg laying and efficiently collecting insect eggs, and can more accurately attract and collect target insects, which has important significance for the development of entomological research, pest control and related ecological fields. It has important significance and positive driving role; at the same time, it also provides a solid theoretical foundation and technical means for subsequent insect resistance research, which is conducive to more accurate acquisition of a large number of specific insect eggs, and provides sufficient experimental sample basis for conducting resistance research; it can better simulate the egg-laying behavior and state of insects in the natural environment, making the data of resistance research more authentic and reliable; it can effectively help researchers analyze and grasp the relationship between the egg-laying patterns of insects and changes in resistance under the influence of different environmental factors such as light and odor, thereby providing strong support for in-depth exploration of the formation mechanism and development trend of insect resistance, and further promote insect resistance research to develop in a deeper and more comprehensive direction. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 The three-dimensional structure diagram of the insect egg collector of the utility model.

[0035] Figure 2 This is an exploded view of the insect egg collector of the utility model (insect attracting light source + cylindrical tube + egg laying barrel + flavor source barrel).

[0036] Figure 3 The three-dimensional structure diagram (stereoscopic diagram) of the insect egg collector of the utility model is shown in FIG.

[0037] Figure 4 This is an exploded view of the insect egg collector of the utility model (cover body + insect attracting light source + cylindrical tube + egg laying barrel + flavor source barrel).

[0038] Figure 5 This is an exploded view of the insect egg collector of the utility model (cover body + insect attracting light source + cylindrical tube).

[0039] Figure 6 The utility model is a cross-sectional schematic diagram of an egg laying barrel in an insect egg collector.

[0040] Figure 7 The three-dimensional structure diagram of the egg laying barrel in the insect egg collector of the utility model.

[0041] Figure 8 The utility model is a cross-sectional schematic diagram of an egg-laying bucket and a flavor source bucket in an insect egg collector.

[0042] Fig. 9 The three-dimensional structure diagram of the flavor source barrel in the insect egg collector of the utility model. DETAILED DESCRIPTION

[0043] The present invention is further described in detail below in conjunction with the embodiments. It should be pointed out that the embodiments described below are intended to facilitate the understanding of the present invention and do not have any limiting effect on the present invention.

[0044] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0045] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0046] The present invention is further described below through specific embodiments.

[0047] In the utility model, it is known that the wingspan of an adult fall armyworm is about 3.2-4.0 cm, the body length is about 1.2-1.6 cm, and usually 100-200 eggs are piled into blocks (in layers), and the diameter of the egg block is about 0.5-1.5 cm; the wingspan of an adult beet armyworm is about 2.5-3.0 cm, the body length is about 1.0-1.4 cm, and usually 10-30 eggs are piled into blocks, and the diameter of the egg block is about 0.3-1.5 cm; the wingspan of an adult fall armyworm is about 3.5-4.6 cm, the body length is about 1.4-2.0 cm, and usually 100-300 eggs are piled into blocks, and the diameter of the egg block is about 0.8-1.8 cm.

[0048] Embodiment 1 A new insect egg collector provided by the utility model

[0049] 1. Basic structure of the egg collector

[0050] In order to solve the problem of difficulty and low efficiency in obtaining test insects in current pesticide resistance monitoring, the utility model provides a new insect egg collector (automatic egg collection device). Through the device, various pests can be successfully lured into the device to lay eggs, and egg masses can be collected to obtain test insects, which greatly improves the efficiency and timeliness of resistance monitoring work and provides a more convenient and effective way for the research and practice of pesticide resistance of related insects.

[0051] The egg collector provided in this embodiment is as follows: Figure 1-Figure 9 As shown, from Figure 1-Figure 9 It can be seen that the insect egg collector includes an insect egg collection bucket 1 and a flavor source bucket 2.

[0052] The egg collecting barrel 1 is made of transparent organic glass, and its components include a cover body 3, an egg laying barrel 4 and a cylindrical tube 5 (in the present embodiment, the cylindrical tube 5 is a cylindrical paper tube, but it can be understood that the material of the cylindrical tube 5 can be set according to different needs and is not limited to paper tubes), and the cover body 3 is a circular upper cover.

[0053] The cover body 3 is composed of an upper and lower layer, the upper layer is a circular cover body layer with a larger diameter, which is 15-20 cm (in this embodiment, specifically 16 cm), and the lower layer is a circular cover body layer with a smaller diameter, which is 13-18 cm (in this embodiment, specifically 15 cm). The upper and lower layers are tightly combined to form a complete cover body 3 structure, as shown in the figure. Figure 4 As shown. The diameter of the upper circular cover layer is relatively large, which can completely cover the opening of the cylindrical tube 5, playing a role of sealing and protection; the diameter of the lower circular cover layer is relatively small, which matches the diameter of the opening of the cylindrical tube 5, and can be tightly nested at the opening of the cylindrical tube 5, further enhancing the sealing effect. In the utility model, the main function of the cover 3 is to prevent insects from escaping, and at the same time facilitate the placement and removal of the insect attractant light source 6. Specifically as Figure 3 As shown, Figure 3 In the embodiment, the sizes of the cover body 3 and the cylindrical tube 5 are inconsistent. This is because in this embodiment, the thickness of the cylindrical tube 5 can be selectively set according to the needs; although the thickness of the cylindrical tube 5 is set to be about 1-2 cm (specifically, Figure 6 As shown, Figure 6 In the cross-sectional view, the thickness of the cylindrical tube 5 is 1.5 cm), but in this embodiment, the cylindrical tube 5 is selected to be a cylindrical paper tube, so the actual thickness is not obvious, and it matches the diameter of the lower circular cover layer, and there is no conflict between the two. In actual applications, the specific thickness of the cylindrical tube 5 can be set according to needs.

[0054] The egg-laying bucket 4 is cylindrical, 25-35 cm high (in this embodiment, specifically 30.5 cm) and 20-30 cm in diameter (in this embodiment, specifically 25 cm). Four strip-shaped insect holes 7 with a diameter of 0.5-5 cm (in this embodiment, specifically 4.0 cm) and a height of 15-25 cm (in this embodiment, specifically 19 cm) are evenly distributed around the wall of the egg-laying bucket 4. The four strip-shaped insect holes 7 are in the middle horizontal position in the height direction of the egg-laying bucket 4. The vertical distance from the strip-shaped insect hole 7 to the top of the egg-laying bucket 4 is equal to the vertical distance from the strip-shaped insect hole 7 to the bottom of the egg-laying bucket 4. Its function is to provide an entry channel for insects to facilitate insects to enter the egg-laying bucket 4. There are 10-30 small holes 8 with a diameter of 0.1-1 cm (in this embodiment, specifically 0.3 cm) distributed at the bottom of the egg-laying barrel 4. The small holes 8 are helpful for the discharge of excess water to ensure the suitability of the environment in the barrel. Such a setting not only creates good ventilation and drainage conditions for insects, but also can fully release the smell of the sweet and sour liquid in the flavor source barrel 2, and with the cooperation of the insect-attracting light source 6, further optimizes the insect's egg-laying environment, thereby attracting more insects to enter the egg-laying barrel 4 to lay eggs; the size of the small holes 8 is smaller than the size of the adult and the egg mass, so as to prevent insects from entering the flavor source barrel 2. source barrel 2 and prevent egg masses from falling into the flavor source barrel 2; and the subsequent screening experiment results show that, in this embodiment, it is preferred to evenly set 18 small holes 8 at the bottom of the egg-laying barrel 4 (below the insect activity area 10). When 18 small holes 8 are set at the bottom of the egg-laying barrel 4, the effect of the sweet and sour liquid in the lower flavor source barrel 2 can be maximized to attract pests into the egg-laying barrel 4; at the same time, limiting the optimal number and aperture of the small holes 8 can also prevent insects from entering the flavor source barrel 2, so that more insects gather in the egg-laying barrel 4 to lay eggs and move, thereby further improving the egg-laying efficiency of pests.

[0055] A cylindrical tube 5 is also detachably fixed in the egg-laying barrel 4. In this embodiment, the cylindrical tube 5 is specifically a cylindrical paper tube with a diameter of 10-20 cm and a height of 25-35 cm (in this embodiment, the specific diameter is 15 cm and the height is 30 cm); 8 rows of round holes 9 are cut out on the side barrel surface of the cylindrical tube 5, which are evenly and equidistantly arranged up and down, with 8 holes in each row, and each round hole 9 has a diameter of 1 cm, so as to avoid the entry of adult insects under the premise of facilitating the overflow of appropriate light. Among them, 4 rows of round holes 9 need to be aligned with the long strip insect entrance hole 7. Such an arrangement can make the light of the insect-attracting light source 6 in the center of the cylindrical tube 5 overflow more smoothly, so as to facilitate the smooth entry of the lured male and female adults. The purpose of setting the cylindrical barrel 5 is to serve as the insertion position of the insect-attracting light source 6 (LED lamp tube or special insect-killing lamp tube or special Noctuidae insect-attracting lamp light source or other light source), so as to provide a more suitable egg-laying environment for insects. The arrangement of the cylindrical barrel 5 aligned with the long strip insect-entry hole 7 is conducive to using suitable light to attract more insects into the egg-laying barrel 4, further enhancing the luring effect. The inside of the cylindrical barrel 5 is evenly and spacedly coated with an appropriate amount of attractant (such as 2-5mL of plant-based attractant) and synergist, and the volume ratio of the plant-based attractant to the synergist is 1:2. Plant-based attractants can release simulated plant scents that insects like, attracting insects to approach, and the synergist of the utility model can significantly enhance this attraction. When the smell of the plant-based attractant is mixed with the synergist, the cylindrical tube can be simulated into the environmental conditions of plant leaves, better simulating the natural environment familiar to insects, thereby greatly enhancing the attractiveness to insects and promoting insects to lay eggs on the cylindrical tube, thereby facilitating the subsequent collection of egg masses. The egg masses can be collected by simply removing or replacing the cylindrical tube, significantly improving the egg-laying efficiency and egg mass collection capacity.

[0056] At the same time, the cylindrical tube in the utility model is preferably a cylindrical paper tube. After taking out the cylindrical paper tube, the paper with the egg mass can be directly cut off and placed in a culture dish for incubation. Subsequent experimental results show that the method can significantly shorten the time required for incubation. At the same time, the use of a cylindrical paper tube also has obvious advantages: compared with the commonly used tools such as egg shovels and egg scrapers, the utility model uses a cylindrical paper tube, which can directly cut off the paper with the egg mass and place it in a culture dish for incubation, thereby avoiding the damage to the egg mass caused by directly using the egg scraper to scrape the egg mass, thereby further improving the ability to collect egg masses and improving the hatching rate.

[0057] In subsequent screening experiments, the position, distribution, number and size of the circular holes 9 cut on the side barrel surface of the cylindrical tube 5 were further screened. The results showed that the above-mentioned position distribution, number and size settings can maximize the attraction effect on insects, while also ensuring sufficient light transmission to provide insects with a suitable egg-laying environment; in addition, the design can also prevent excessive light from entering the egg-laying barrel 4, thereby preventing interference with the insect's activities or egg-laying behavior.

[0058] In this embodiment, a common 3-5 watt LED light source is used as the insect attracting light source 6, specifically 4 watts. Under this light intensity, on the one hand, it can attract various insects to the greatest extent, and on the other hand, it is also very beneficial for insects to lay eggs. Specifically, this light intensity and light source characteristics can effectively stimulate the phototaxis instinct of insects, prompting them to approach the light source, thereby increasing the probability of insects being attracted to the egg collection bucket 1; at the same time, the light conditions can also create an environment atmosphere suitable for insect reproduction, play a positive role in promoting insect egg laying, help improve the efficiency and quantity of insect egg laying, and provide more abundant resources for subsequent insect resistance research and application; the insect attracting light source 6 can be detachably installed in the cylindrical tube 5. By precisely controlling the intensity of the insect-attracting light source 6 and selecting the best formula of the attractants (plant-based attractants and enhancers) coated at intervals on the inner wall of the cylindrical tube 5, and at the same time combining the attraction of the sweet and sour liquid in the flavor source barrel 2 to insects, the three are used in combination, and continuous testing, analysis and optimization adjustments are carried out in subsequent verification experiments to determine the most suitable combination of conditions for different insects, thereby maximizing the insect-attracting effect, making the insects show a higher tendency and willingness to enter the insect egg collection device (insect egg collector) of the utility model, thereby achieving more efficient insect trapping.

[0059] The cylindrical barrel 5 divides the interior of the egg-laying barrel 4 into multiple functional areas, including an insect activity area 10, a light source area 11 and an egg-laying area 12. The insect activity area 10 is the area between the egg-laying barrel 4 and the cylindrical barrel 5, which provides sufficient space for insect activities, so that they can move freely and find egg-laying positions; the light source area 11 is the area inside the cylindrical barrel 5, and the insects are attracted to enter the egg-laying barrel 4 by setting the best lighting conditions of the insect-attracting light source 6 inside the cylindrical barrel 5; the egg-laying area 12 is mainly the outer wall area of ​​the cylindrical barrel 5, and the inner wall of the cylindrical barrel 5 is evenly coated with the best attractant of the utility model, and at the same time, in combination with the insect-attracting light source 6, it fully combines the insect's egg-laying habits to provide a suitable surface and environment for the insect to lay eggs, so as to facilitate the insect to lay eggs, and the egg masses laid by the insect are all in the egg-laying area. The partition design makes the functional division of the insect egg collector of the utility model clearer, avoids interference between different functional areas, provides a suitable activity environment for insects, and also optimizes the activity path of insects in the egg-laying barrel 4, improves the contact opportunity between insects and the egg-laying area 12, thereby increasing the egg-laying amount, and also improves the efficiency and convenience of collecting egg masses; and the partition design facilitates the subsequent classification and processing of the collected egg masses, and improves the convenience of subsequent research or application.

[0060] The flavor source barrel 2 is made of transparent organic glass, with a diameter of 25-30 cm and a height of 3-8 cm. In this embodiment, it is specifically 27 cm in diameter and 5.5 cm in height. The upper surface of the flavor source barrel 2 is provided with a groove 13 suitable for placing the egg-laying barrel 4. The flavor source barrel 2 and the egg-laying barrel 4 are detachably connected together through the groove 13, and the flavor source barrel 2 is fixed to the lower part of the egg-laying barrel 4; the size of the groove 13 is suitable for the size of the egg-laying barrel 4, with a diameter of 20-30 cm (in this embodiment, specifically 25 cm). The sweet and sour liquid is added to the flavor source barrel 2. On the one hand, because insects are more inclined to lay eggs in a humid environment, adding sweet and sour liquid is more conducive to creating environmental conditions suitable for insect growth; on the other hand, adding sweet and sour liquid can significantly enhance the attraction effect on insects, and cooperate with the insect-attracting light source 6 and the attractant (plant-based attractant + synergist) on the inner wall of the cylindrical tube 5, it can further promote the attraction of insects, thereby effectively attracting male and female Lepidoptera adults to enter the egg-laying barrel 4. The centers of the egg-laying barrel 4, the cylindrical tube 5 and the flavor source barrel 2 are on the same connecting line, and the connecting line is perpendicular to the horizontal plane. The above arrangement can significantly enhance the structural stability and functional coordination of the entire device (worm egg collector), thereby ensuring the smooth progress of the luring and collection process.

[0061] In the present invention, an attractant is used in combination with an insect attractant light source 6 to effectively attract insects, including a sweet and sour liquid placed in a flavor source barrel 2, and a plant-based attractant and a synergist coated on the inner wall of a cylindrical tube 5 at intervals. The formulas of different attractants are specifically as follows:

[0062] (1) Sweet and sour liquid: placed in the flavor source barrel 2, using brown sugar (pure brown sugar), vinegar (4% Hengshun vinegar), wine (52% Erguotou), water in a mass ratio of 1:8:3:50. When in use, place the prepared sweet and sour liquid in the flavor source barrel 2, preferably occupying 1 / 2 of the volume of the container (approximately 1574 mL);

[0063] (2) Plant attractant: The basic component is fresh juice of plants such as corn, cabbage, and taro; the synergist is evenly coated on the inner wall of the cylindrical tube 5 at intervals. The amount of the plant attractant added is 2-5 mL, and in this embodiment, it is specifically 4 mL;

[0064] (3) Synergists: specifically, any one or more of the following compounds: (Z)-3-hexenyl acetate (cis-3-hexenyl acetate), (E)-2-hexenal (trans-2-hexenal), C 9 H 18 O (nonanal), 3-buten-1-ylisothiocyanate (3-butenyl isothiocyanate); and the plant-derived attractant is evenly coated on the inner wall of the cylindrical tube 5 at intervals. The amount of the enhancer added is 4-10mL, and in this embodiment, it is specifically 10mL.

[0065] When the attractant on the inner wall of the cylindrical tube is coated in different ways, there are obvious differences in the ability to attract different insects. Compared with the mixture of plant-based attractant + synergist, the use of plant-based attractant and synergist coated on the inner wall of the cylindrical tube at intervals has a more obvious attracting effect on adult insects. This may be because, on the one hand, the interval coating may cause the plant-based attractant and synergist to form higher concentrations in local areas. The higher concentration of signal molecules is more easily perceived and responded to by adult insects, thereby enhancing the attraction to adult insects. The components in the mixture may dilute each other, reducing the concentration of the active ingredients, weakening its signal strength, and thus affecting the attracting effect; on the other hand, in the mixture, , the molecules of plant-derived attractants and synergists may compete with each other for binding sites with adult insects, and intermittent coating can reduce this competition, allowing each to play a more effective role, so that the synergist can more accurately enhance the odor characteristics of the plant-derived attractant, thereby improving the attracting effect on adult insects; at the same time, although plant-derived attractants and synergists can also play a certain synergistic role in the mixture, intermittent coating may create conditions that are more conducive to their synergistic effect. This specific arrangement may make it easier for adult insects to perceive the presence of attractants and synergists, thereby improving the attracting effect. Therefore, it is not necessary to simply mix attractants and synergists together to achieve the best effect. Instead, it is necessary to deeply explore and optimize their combination methods and coating strategies to maximize the attracting effect on adults, thereby maximizing the ability to collect egg masses. The reason is that the perception and behavioral response mechanisms of insects are very complex, and it is necessary to comprehensively consider the impact of multiple factors on them, and achieve the best attracting effect through fine regulation and optimization. When different synergists are used, the attracting effect on different insects is also significantly different: for fall armyworm, when the three synergists of cis-3-hexenyl acetate, trans-2-hexenal and nonanal are used, the attracting effect is ideal, and when the mass ratio of the three compounds is 1:0.5:2, the attracting effect on fall armyworm is the best, which may be because the odor characteristics produced by this combination are more consistent with the perception signals of fall armyworm to food sources or habitats; while for beet armyworm and fall armyworm, when the three synergists of cis-3-hexenyl acetate, trans-2-hexenal and 3-butenyl isothiocyanate are used, the attracting effect is ideal, and when the mass ratio of the three compounds is 20:5:1, the attracting effect on beet armyworm and fall armyworm is the best, which may be because it can better trigger their specific olfactory response. The combination and ratio of different synergists will significantly affect the attracting effect on different insects, which reflects the specificity and complexity of different insects in olfactory perception and behavioral response. Through in-depth research and precise formulation of attractant combinations, the ability to attract specific insects can be improved in a more targeted manner.

[0066] Different plant-based attractants have different abilities to attract different insects. For fall armyworm, fresh juice of corn plants showed a relatively strong attractant effect when used as a plant-based attractant, which may be because fall armyworm has a special sensitivity and tendency to the smell of corn; while for beet armyworm and armyworm, fresh juice of asparagus, cabbage and taro plants had a more significant attractant effect, which may be because the olfactory perception system of beet armyworm and armyworm has a unique recognition and response mechanism for the chemical signals of asparagus, cabbage and taro.

[0067] Therefore, it can be seen that different insects have their own unique preferences and reactions to plant-based attractants. In practical applications, it is necessary to select and optimize the composition and proportion of plant-based attractants according to the type of target insects in order to achieve the best attractant effect.

[0068] 2. Method of using the insect egg collector of the utility model (or the matching luring method)

[0069] When the utility model is used, the egg collector of the utility model first adds 1 / 2 container volume of suitable sweet and sour liquid into the flavor source barrel 2, and utilizes the smell it emits to attract male and female Lepidoptera adults to approach. Then the egg collector is placed between corresponding plant fields (such as corn fields), supported by a tripod, and higher than the top of the corresponding plant, and then the insect attracting light source 6 is placed in the center of the cylindrical barrel 5 (the battery is placed on the ground), and the light intensity of the insect attracting light source 6 is set to be a common 4-watt LED lamp or a special Noctuidae insect attracting light source, and the cylindrical barrel 5 coated with an attractant (plant source attractant + synergist) is taken out at 8 o'clock every morning to recycle the egg mass, and for 3 consecutive days, various egg mass types and quantities are counted, and the egg mass is bred indoors to count the hatching rate. The illumination of suitable intensity can further attract insects to enter the egg-laying barrel 4, and under the dual effects of smell and illumination, the insects are prompted to enter the egg-laying barrel 4 to lay eggs, thereby achieving efficient luring and egg collection effects.

[0070] 3. The functions of different structures in the utility model egg collector

[0071] The utility model provides a new insect egg collector, and its different structures have the following functions:

[0072] 1. Insect egg collecting barrel 1: comprising a cover 3, an egg laying barrel 4, and a cylindrical barrel 5, wherein an insect attracting light source 6 is arranged inside the cylindrical barrel 5; the insect egg collecting barrel 1 as a whole is used to attract insects and collect eggs laid by insects, and provide a specific space for insects to lay eggs;

[0073] 2. Flavor source barrel 2: includes sweet and sour liquid, which releases a specific smell to attract insects, and is used in conjunction with the insect attracting light source 6 to further enhance the insect attraction effect; at the same time, the sweet and sour liquid creates a moist environment, which is more suitable for insect growth and egg laying;

[0074] 3. Cover 3: cooperates with the egg-laying barrel 4 to form a relatively closed space, which prevents insects from escaping and facilitates the placement and removal of the insect-attracting light source 6;

[0075] 4. Egg-laying bucket 4: It is the main place for insects to move and lay eggs;

[0076] 5. Cylindrical barrel 5: It provides support and accommodation space for the internal insect attracting light source 6 and other structures. Its shape is also helpful to guide insects to enter. The circular holes 9 arranged on the side barrel surface can maximize the attraction effect on insects, and at the same time, it can ensure sufficient light to pass through, providing a suitable egg-laying environment for insects. In addition, the design can also prevent too much light from entering the egg-laying barrel 4, thereby preventing interference with the activities or egg-laying behaviors of insects. At the same time, the plant-derived attractant and enhancer are evenly coated inside, and the two are coated at intervals, which can simulate the plant leaf environment, enhance the attraction to insects, promote insects to lay eggs on the cylindrical barrel, and facilitate the subsequent collection of egg masses. And the cylindrical paper tube form is adopted, and the paper with egg masses can be directly cut off for hatching, avoiding the damage to the egg masses when scraping eggs with common tools, thereby improving the egg mass collection capacity and hatching rate.

[0077] 6. Insect-attracting light source 6: Use light of appropriate intensity to attract insects and create lighting conditions that are conducive to insect egg laying;

[0078] 7. Long strip-shaped insect entrance hole 7: provides an entry channel for insects, facilitating insects to enter the egg-laying barrel 4; at the same time, it is conducive to air circulation, providing insects with sufficient oxygen, ensuring the suitability of the environment in the barrel; it can also facilitate the overflow of light and the dissemination of attractant odor, thereby attracting more insects to enter the egg-laying barrel 4 to lay eggs;

[0079] 8. Small hole 8: It helps to drain excess water, ensures a suitable environment in the barrel, creates good drainage conditions for insects, and prevents insects from entering the flavor source barrel 2 and egg masses from falling into the flavor source barrel 2, maximizes the effect of the sweet and sour liquid, cooperates with the insect attracting light source 6, attracts pests to enter the egg-laying barrel 4 to lay eggs, and improves the egg-laying efficiency of pests;

[0080] 9. Round hole 9: to prevent adult insects from entering under the premise of allowing appropriate light to overflow;

[0081] 10. Insect activity area 10: Insect activity area 10 provides sufficient space for insects to move freely and find egg-laying locations;

[0082] 11. Light source area 11: by setting the best lighting conditions of the insect-attracting light source 6, insects are attracted to enter the egg-laying barrel 4;

[0083] 12. Egg-laying area 12: It is the outer wall area of ​​the cylindrical tube 5. It provides a suitable surface and environment for insects to lay eggs, taking into account the egg-laying habits of insects. The eggs laid by insects are all in the egg-laying area, which is also convenient for the subsequent collection of egg masses.

[0084] 13. Groove 13: The flavor source barrel 2 and the egg laying barrel 4 are detachably connected together through the groove 13.

[0085] The present embodiment provides an insect egg collector, which significantly improves the insect attracting effect and the egg collection efficiency through the arrangement of the insect egg collecting barrel 1 (including the cover body 3, the egg laying barrel 4, the cylindrical tube 5 and other structures) and the flavor source barrel 2 and other structures, and cooperates with the matching luring method (such as using the combination of sweet and sour liquid, plant-based attractants or synergists to emit odors to attract insects, and using a specific intensity of insect attracting light source to attract insects and create suitable egg-laying lighting conditions), has the functions of better promoting insect aggregation, inducing insects to lay eggs and efficiently collecting insect eggs, and can more accurately attract and collect target insects, which is of great significance to the development of entomological research, pest control and related ecological fields. It has great significance and positive driving role; at the same time, it also provides a solid theoretical foundation and technical means for subsequent insect resistance research, which is conducive to obtaining a large number of specific insect eggs more accurately, and provides sufficient experimental sample basis for conducting resistance research; it can better simulate the egg-laying behavior and state of insects in the natural environment, making the data of resistance research more authentic and reliable; it can effectively help researchers analyze and grasp the relationship between the egg-laying patterns of insects and changes in resistance under the influence of different environmental factors such as light and odor, thereby providing strong support for in-depth exploration of the formation mechanism and development trend of insect resistance, and further promote insect resistance research to a deeper and more comprehensive direction.

[0086] Example 2 The importance of different attracting factors in the utility model egg collector for collecting fall armyworm eggs

[0087] In this embodiment, in order to further enhance the attracting effect on insects (spodoptera frugiperda) and improve the collection efficiency of insect egg masses (spodoptera frugiperda egg masses), a comparative screening experiment was conducted on different combinations of attracting factors in the egg collector, and the following 12 control treatment groups were set respectively (in this embodiment, except for the conditions in the following different treatment groups, the other conditions are the optimal conditions in Example 1):

[0088] 1. Treatment A: Egg collection bucket + LED light, the light intensity of the LED light is 4 watts;

[0089] 2. Treatment B: egg collection bucket + LED light, the light intensity of the LED light is 2 watts;

[0090] 3. Treatment C: egg collection bucket + LED light, the light intensity of the LED light is 6 watts;

[0091] 4. Treatment D: egg collection bucket + water (flavor source bucket) + plant attractant (4 mL fresh juice from corn plants, evenly coated on the inner wall of the cylindrical tube);

[0092] 5. Treatment E: egg collection bucket + sweet and sour liquid (flavor source bucket) + plant attractant (4 mL fresh juice from corn plants, evenly coated on the inner wall of the cylindrical tube);

[0093] 6. Treatment F: egg collection bucket + sweet and sour liquid (flavor source bucket) + synergist (cis-3-hexene acetate, 10 mg / L, evenly coated on the inner wall of the cylindrical tube);

[0094] 7. Treatment G: egg collection bucket + sweet and sour liquid (flavor source bucket) + synergist (trans-2-hexenal, 10 mg / L, evenly coated on the inner wall of the cylindrical tube);

[0095] 8. Treatment H: egg collection bucket + sweet and sour liquid (flavor source bucket) + synergist (nonanal, 10 mg / L, evenly coated on the inner wall of the cylindrical tube);

[0096] 9. Treatment I: egg collection bucket + sweet and sour liquid (flavor source bucket) + attractant (mixture), the attractant is: a mixture of plant attractant (fresh juice of corn plants) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: nonanal (mass ratio) = 1:0.5:2, 10 mg / L), wherein the volume ratio of the plant attractant to the synergist is 1:1-3, and in this embodiment, specifically 1:2; the mixture is evenly coated on the inner wall of the cylindrical tube;

[0097] 10. Treatment J: egg collection bucket + sweet and sour liquid (flavor source bucket) + attractant, the attractant is: plant attractant (fresh juice of corn plants) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: nonanal (mass ratio) = 1:0.5:2, 10 mg / L), wherein the volume ratio of the plant attractant to the synergist is 1:1-3, and in this embodiment, specifically 1:2; the plant attractant and the synergist are evenly and spaced on the inner wall of the cylindrical tube;

[0098] 11. Treatment K: egg collection bucket + sweet and sour liquid (flavor source bucket) + attractant (mixture) + LED light, the attractant is: a mixture of plant attractant (fresh juice of corn plants) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: nonanal (mass ratio) = 1:0.5:2, 10 mg / L), wherein the volume ratio of the plant attractant to the synergist is 1:1-3, and in this embodiment, specifically 1:2; the mixture is evenly coated on the inner wall of the cylindrical tube; the light intensity of the LED light is 4 watts;

[0099] 12. Treatment L: insect egg collection bucket + sweet and sour liquid (flavor source bucket) + attractant + LED light, the attractant is: plant attractant (fresh juice of corn plants) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: nonanal (mass ratio) = 1:0.5:2, 10 mg / L), wherein the volume ratio of the plant attractant to the synergist is 1:1-3, specifically 1:2 in this embodiment; the plant attractant and the synergist are evenly and spaced on the inner wall of the cylindrical tube; the light intensity of the LED lamp is 4 watts.

[0100] The following operation was performed: In August, an egg induction experiment was conducted in a corn field in Xindai Town, Pinghu City. At this time, the corn was in the jointing stage. The devices of twelve treatments A to L (including 3 replicates) were placed in the center of the corn field (an area of ​​120 mu), about 20 meters apart from each other. The egg collection bucket was supported by a tripod and higher than the top of the corn plant. First, a cylindrical paper tube with a circular hole cut into it was placed in the egg collection bucket, and then an LED lamp was placed in the center of the cylindrical paper tube (the battery was placed on the ground). The egg masses were collected at 8 o'clock every morning for 3 consecutive days, and the types and quantities of various egg masses were counted. They were then bred indoors and the hatching rate was counted.

[0101] Table 1 Number of egg masses of fall armyworm

[0102]

[0103] Note: Different lowercase letters in the same column indicate significant differences (Duncan's new multiple range method, P<0.05).

[0104] The effects of different treatment groups in attracting fall armyworm eggs are shown in Table 1. The results show that the utility model egg collector and the matching attracting method have excellent egg mass collection capabilities:

[0105] (1) The results of treatments A to C showed that different light intensities had different effects on the egg mass collection capacity. When the light intensity was 2-6 watts, there was no significant difference in the egg mass collection capacity among different groups. However, when the light intensity was 4 watts, the egg mass collection capacity reached the highest level, and the amount of eggs collected was the highest.

[0106] (2) The results of treatments D to E show that the sweet and sour liquid has a good ability to attract insects. When water is used to replace the sweet and sour liquid, the attracting effect on adult insects is poor. This shows that the specific chemical components and odor characteristics contained in the sweet and sour liquid play a vital role in attracting insects. The sugar and acidic substances in the sweet and sour liquid may form unique chemical signals that can be keenly perceived and recognized by insects, thereby triggering their tendency to behave. Water lacks these specific chemical components and odor information, and cannot effectively activate the insect's sensory system and foraging instinct, resulting in a decrease in the attracting effect on adult insects.

[0107] (3) The results of treatments E to J showed that different attractants had different abilities to collect egg masses. When only sweet and sour liquid was used in combination with a single plant-based attractant or synergist, the attracting effect on adult insects was poor, indicating that only a single attractant was not attractive enough to adult insects. When a mixture of plant-based attractants and synergists was used, the attracting effect on adult insects was enhanced to a certain extent compared to a single attractant. This indicates that adding sweet and sour liquid and a suitable synergist has a certain synergistic effect on egg mass collection.

[0108] When plant-derived attractants and enhancers were evenly and spaced on the inner wall of the cylindrical tube, the results showed that compared with the mixture of plant-derived attractants + enhancers, the attracting effect on adult insects was improved. This may be because, on the one hand, the interval coating may cause the plant-derived attractants and enhancers to form higher concentrations in local areas, which is consistent with the concentration-dependent characteristics of chemical signal transmission. Higher concentrations of signal molecules are more easily perceived and responded to by adult insects, thereby enhancing the attraction to adult insects. The components in the mixture may dilute each other, reducing the concentration of the active ingredients and weakening the signal intensity, which in turn affects Attraction effect; on the other hand, in the mixture, the molecules of plant-derived attractants and synergists may compete with each other for binding sites with adults, and intermittent coating can reduce this competition, allowing each to play a more effective role, so that the synergist can more accurately enhance the odor characteristics of the plant-derived attractant, thereby improving the attracting effect on adults; at the same time, although plant-derived attractants and synergists can also play a certain synergistic role in the mixture, intermittent coating may create conditions that are more conducive to their synergistic effect. This specific arrangement may make it easier for adults to perceive the presence of attractants and synergists, thereby improving the attracting effect. Therefore, it is not necessary to simply mix attractants and synergists together to achieve the best effect. Instead, it is necessary to deeply explore and optimize their combination methods and coating strategies to achieve the highest attracting effect on adults, thereby maximizing the ability to collect egg masses. The reason is that the perception and behavioral response mechanisms of insects are very complex, and it is necessary to comprehensively consider the effects of multiple factors on them, and achieve the best attracting effect through fine regulation and optimization.

[0109] At the same time, in this embodiment, the volume ratio of the plant-derived attractant and the synergist was also screened, and the results showed that when the volume ratio of the plant-derived attractant (fresh juice of corn plants) and the synergist (cis-3-hexene acetate: trans-2-hexenal: nonanal (mass ratio) = 1:0.5:2, 10 mg / L) was 1:1-3, it had a good ability to attract adults, and when the volume ratio of the two was 1:2, the ability to attract adults reached the highest, which may be because different ratios will produce different effects. When the volume ratio of the two is appropriate, the synergist can fully enhance the effect of the plant-derived attractant, making the odor signal it releases stronger and more attractive; if the ratio of the synergist is too low, the enhancement effect may not be fully exerted; and if the ratio is too high, it may cover up or interfere with the original attraction of the plant-derived attractant to insects to a certain extent, or produce some unexpected effects. Therefore, in this embodiment, the volume ratio of the plant-derived attractant and the synergist is selected to be 1:1-3, and preferably 1:2.

[0110] (4) The results of treatments I to L showed that compared with the ability to attract adults and collect egg masses in treatments I and J, the egg mass collection ability of the egg collector was significantly enhanced after the addition of the insect attractant light source, which indicates that the combination of a suitable insect attractant light source + sweet and sour liquid (flavor source bucket) + attractant (plant-derived attractant (fresh juice of corn plants) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: nonanal (mass ratio) = 1:0.5:2, 10 mg / L)) has the most attractant activity, indicating that the combination of a suitable attractant (plant-derived attractant + synergist) has a significant synergistic effect on the egg mass collection ability of the insect attractant light source. At the same time, the attractant effect of treatment A was second, indicating that the insect attractant light source has a significant role in attracting adults and laying eggs.

[0111] At the same time, the analysis of egg mass hatching rate showed that no matter which of the above treatments was adopted, the indoor hatching rate of the collected egg masses was 52-87%, that is, all above 50%. Therefore, in treatment L, one device can collect more than 300 effective eggs (insect quantity) per day, which is sufficient for monitoring the resistance of fall armyworm.

[0112] Example 3 The importance of different attractants in the utility model egg collector for collecting beet armyworm eggs

[0113] In this embodiment, in order to further enhance the attracting effect on insects (beet armyworm) and improve the collection efficiency of insect egg masses (beet armyworm egg masses), a comparative screening experiment was conducted on different combinations of attracting factors in the egg collector, and the following 12 control treatment groups were set respectively (in this embodiment, except for the conditions in the following different treatment groups, the other conditions are the optimal conditions in Example 1):

[0114] 1. Treatment A: Egg collection bucket + LED light, the light intensity of the LED light is 4 watts;

[0115] 2. Treatment B: egg collection bucket + LED light, the light intensity of the LED light is 2 watts;

[0116] 3. Treatment C: egg collection bucket + LED light, the light intensity of the LED light is 6 watts;

[0117] 4. Treatment D: egg collection bucket + water (flavor source bucket) + plant attractant (4 mL fresh asparagus juice, evenly coated on the inner wall of the cylindrical tube);

[0118] 5. Treatment E: egg collection bucket + sweet and sour liquid (flavor source bucket) + plant attractant (4 mL fresh asparagus juice, evenly coated on the inner wall of the cylindrical tube);

[0119] 6. Treatment F: egg collection bucket + sweet and sour liquid (flavor source bucket) + synergist (cis-3-hexene acetate, 10 mg / L, evenly coated on the inner wall of the cylindrical tube);

[0120] 7. Treatment G: egg collection bucket + sweet and sour liquid (flavor source bucket) + synergist (trans-2-hexenal, 10 mg / L, evenly coated on the inner wall of the cylindrical tube);

[0121] 8. Treatment H: egg collection bucket + sweet and sour liquid (flavor source bucket) + synergist (3-butenyl isothiocyanate, 10 mg / L, evenly coated on the inner wall of the cylindrical tube);

[0122] 9. Treatment I: egg collection bucket + sweet and sour liquid (flavor source bucket) + attractant (mixture), the attractant is: a mixture of plant attractant (fresh juice of tender asparagus) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L), wherein the volume ratio of the plant attractant to the synergist is 1:1-3, and in this embodiment, specifically 1:2; the mixture is evenly coated on the inner wall of the cylindrical tube;

[0123] 10. Treatment J: egg collection bucket + sweet and sour liquid (flavor source bucket) + attractant, the attractant is: plant-derived attractant (fresh juice of tender asparagus) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L), wherein the volume ratio of the plant-derived attractant to the synergist is 1:1-3, and in this embodiment, specifically 1:2; the plant-derived attractant and the synergist are evenly and spaced on the inner wall of the cylindrical tube;

[0124] 11. Treatment K: egg collection bucket + sweet and sour liquid (flavor source bucket) + attractant (mixture) + LED light, the attractant is: a mixture of plant attractant (fresh juice of tender asparagus) + synergist (cis-3-hexene acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L), wherein the volume ratio of the plant attractant to the synergist is 1:1-3, and in this embodiment, specifically 1:2; the mixture is evenly coated on the inner wall of the cylindrical tube; the light intensity of the LED light is 4 watts;

[0125] 12. Treatment L: egg collection bucket + sweet and sour liquid (flavor source bucket) + attractant + LED light, the attractant is: plant attractant (fresh juice of tender asparagus) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L), wherein the volume ratio of the plant attractant to the synergist is 1:1-3, specifically 1:2 in this embodiment; the plant attractant and the synergist are evenly and spaced on the inner wall of the cylindrical tube; the light intensity of the LED lamp is 4 watts.

[0126] The following operations were performed: In August, an egg induction experiment was conducted at Jiaxing Shuiyuewan Agricultural Technology Co., Ltd., where greenhouse asparagus and leafy vegetables were grown. Beet armyworm and Spodoptera litura were the main lepidopteran pests in this season. The 12 treatments (including 3 replicates) were placed between the asparagus greenhouses, about 15 meters apart. The egg collection bucket was supported by a tripod and was basically flush with the vents on both sides of the greenhouse. First, a cylindrical paper tube with a circular hole cut into it was placed in the egg collection bucket, and then an LED lamp was placed in the center of the cylindrical paper tube (the battery was placed on the ground). The egg masses were collected at 8 a.m. every day for 3 consecutive days, and the types and quantities of various egg masses were counted. They were then bred indoors and the hatching rate was counted.

[0127] Table 2 Number of egg masses of beet armyworm

[0128]

[0129] Note: Different lowercase letters in the same column indicate significant differences (Duncan's new multiple range method, P<0.05).

[0130] The effects of different treatment groups in attracting beet armyworm eggs are shown in Table 2. The results show that the utility model egg collector and the matching attracting method have excellent egg mass collection capabilities:

[0131] (1) The results of treatments A to C showed that different light intensities had different effects on the egg mass collection capacity. When the light intensity was 2-6 watts, there was no significant difference in the egg mass collection capacity among different groups. However, when the light intensity was 4 watts, the egg mass collection capacity reached the highest level, and the amount of eggs collected was the highest.

[0132] (2) The results of treatments D to E show that the sweet and sour liquid has a good ability to attract insects. When water is used to replace the sweet and sour liquid, the attracting effect on adult insects is poor. This shows that the specific chemical components and odor characteristics contained in the sweet and sour liquid play a vital role in attracting insects. The sugar and acidic substances in the sweet and sour liquid may form unique chemical signals that can be keenly perceived and recognized by insects, thereby triggering their tendency to behave. Water lacks these specific chemical components and odor information, and cannot effectively activate the insect's sensory system and foraging instinct, resulting in a decrease in the attracting effect on adult insects.

[0133] (3) The results of treatments E to J showed that different attractants had different abilities to collect egg masses. When only sweet and sour liquid was used in combination with a single plant-based attractant or synergist, the attracting effect on adult insects was poor, indicating that only a single attractant was not attractive enough to adult insects. When a mixture of plant-based attractants and synergists was used, the attracting effect on adult insects was enhanced to a certain extent compared to a single attractant. This indicates that adding sweet and sour liquid and a suitable synergist has a certain synergistic effect on egg mass collection.

[0134] When plant-derived attractants and enhancers were evenly and spaced on the inner wall of the cylindrical tube, the results showed that compared with the mixture of plant-derived attractants + enhancers, the attracting effect on adult insects was improved. This may be because, on the one hand, the interval coating may cause the plant-derived attractants and enhancers to form higher concentrations in local areas, which is consistent with the concentration-dependent characteristics of chemical signal transmission. Higher concentrations of signal molecules are more easily perceived and responded to by adult insects, thereby enhancing the attraction to adult insects. The components in the mixture may dilute each other, reducing the concentration of the active ingredients and weakening the signal intensity, which in turn affects Attraction effect; on the other hand, in the mixture, the molecules of plant-derived attractants and synergists may compete with each other for binding sites with adults, and intermittent coating can reduce this competition, allowing each to play a more effective role, so that the synergist can more accurately enhance the odor characteristics of the plant-derived attractant, thereby improving the attracting effect on adults; at the same time, although plant-derived attractants and synergists can also play a certain synergistic role in the mixture, intermittent coating may create conditions that are more conducive to their synergistic effect. This specific arrangement may make it easier for adults to perceive the presence of attractants and synergists, thereby improving the attracting effect. Therefore, it is not necessary to simply mix attractants and synergists together to achieve the best effect. Instead, it is necessary to deeply explore and optimize their combination methods and coating strategies to achieve the highest attracting effect on adults, thereby maximizing the ability to collect egg masses. The reason is that the perception and behavioral response mechanisms of insects are very complex, and it is necessary to comprehensively consider the effects of multiple factors on them, and achieve the best attracting effect through fine regulation and optimization.

[0135] At the same time, in this embodiment, the volume ratio of the plant-derived attractant and the synergist was also screened, and the results showed that when the volume ratio of the plant-derived attractant (fresh asparagus juice) and the synergist (cis-3-hexene acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L) was 1:1-3, it had a good ability to attract adults, and when the volume ratio of the two was 1:2, the ability to attract adults reached the highest, which may be because different ratios will produce different effects. When the volume ratio of the two is appropriate, the synergist can fully enhance the effect of the plant-derived attractant, making the odor signal it releases stronger and more attractive; if the ratio of the synergist is too low, the enhancement effect may not be fully exerted; and if the ratio is too high, it may cover up or interfere with the original attraction of the plant-derived attractant to insects to a certain extent, or produce some unexpected effects. Therefore, in this embodiment, the volume ratio of the plant-derived attractant and the synergist is selected to be 1:1-3, and preferably 1:2.

[0136] (4) The results of treatments I to L showed that compared with the ability to attract adults and collect egg masses in treatments I and J, the egg mass collection ability of the egg collector was significantly enhanced after the addition of the insect attractant light source, which indicates that the combination of a suitable insect attractant light source + sweet and sour liquid (flavor source bucket) + attractant (plant-derived attractant (fresh asparagus juice) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L)) has the most attractant activity, indicating that the combination of a suitable attractant (plant-derived attractant + synergist) has a significant synergistic effect on the egg mass collection ability of the insect attractant light source. At the same time, the attractant effect of treatment A was second, indicating that the insect attractant light source has a significant role in attracting adults and laying eggs.

[0137] At the same time, the analysis of the egg mass hatching rate of beet armyworm showed that the indoor hatching rate of the egg masses collected in the 13 treatments was 65-93%, which was in line with the expected egg mass hatching rate expectations. In treatment L, one device could collect more than 500 effective eggs (insects) per day, which was sufficient for monitoring the resistance of beet armyworm.

[0138] Example 4 The importance of different attracting factors in the utility model egg collector for collecting egg masses of Spodoptera litura

[0139] In this embodiment, in order to further enhance the attracting effect on insects (Spodoptera litura) and improve the collection efficiency of insect egg masses (Spodoptera litura egg masses), a comparative screening experiment was conducted on different combinations of attracting factors in the egg collector, and the following 12 control treatment groups were set respectively (in this embodiment, except for the conditions in the following different treatment groups, the other conditions are the optimal conditions in Example 1):

[0140] 1. Treatment A: Egg collection bucket + LED light, the light intensity of the LED light is 4 watts;

[0141] 2. Treatment B: egg collection bucket + LED light, the light intensity of the LED light is 2 watts;

[0142] 3. Treatment C: egg collection bucket + LED light, the light intensity of the LED light is 6 watts;

[0143] 4. Treatment D: egg collection bucket + water (flavor source bucket) + plant attractant (4 mL fresh asparagus juice, evenly coated on the inner wall of the cylindrical tube);

[0144] 5. Treatment E: egg collection bucket + sweet and sour liquid (flavor source bucket) + plant attractant (4 mL fresh asparagus juice, evenly coated on the inner wall of the cylindrical tube);

[0145] 6. Treatment F: egg collection bucket + sweet and sour liquid (flavor source bucket) + synergist (cis-3-hexene acetate, 10 mg / L, evenly coated on the inner wall of the cylindrical tube);

[0146] 7. Treatment G: egg collection bucket + sweet and sour liquid (flavor source bucket) + synergist (trans-2-hexenal, 10 mg / L, evenly coated on the inner wall of the cylindrical tube);

[0147] 8. Treatment H: egg collection bucket + sweet and sour liquid (flavor source bucket) + synergist (3-butenyl isothiocyanate, 10 mg / L, evenly coated on the inner wall of the cylindrical tube);

[0148] 9. Treatment I: egg collection bucket + sweet and sour liquid (flavor source bucket) + attractant (mixture), the attractant is: a mixture of plant attractant (fresh juice of tender asparagus) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L), wherein the volume ratio of the plant attractant to the synergist is 1:1-3, and in this embodiment, specifically 1:2; the mixture is evenly coated on the inner wall of the cylindrical tube;

[0149] 10. Treatment J: egg collection bucket + sweet and sour liquid (flavor source bucket) + attractant, the attractant is: plant-derived attractant (fresh juice of tender asparagus) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L), wherein the volume ratio of the plant-derived attractant to the synergist is 1:1-3, and in this embodiment, specifically 1:2; the plant-derived attractant and the synergist are evenly and spaced on the inner wall of the cylindrical tube;

[0150] 11. Treatment K: egg collection bucket + sweet and sour liquid (flavor source bucket) + attractant (mixture) + LED light, the attractant is: a mixture of plant attractant (fresh juice of tender asparagus) + synergist (cis-3-hexene acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L), wherein the volume ratio of the plant attractant to the synergist is 1:1-3, and in this embodiment, specifically 1:2; the mixture is evenly coated on the inner wall of the cylindrical tube; the light intensity of the LED light is 4 watts;

[0151] 12. Treatment L: egg collection bucket + sweet and sour liquid (flavor source bucket) + attractant + LED light, the attractant is: plant attractant (fresh juice of tender asparagus) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L), wherein the volume ratio of the plant attractant to the synergist is 1:1-3, specifically 1:2 in this embodiment; the plant attractant and the synergist are evenly and spaced on the inner wall of the cylindrical tube; the light intensity of the LED lamp is 4 watts.

[0152] And according to the following operation: Since the farm of Jiaxing Shuiyuewan Agricultural Technology Co., Ltd. grows a variety of crops, and August is also the high-incidence season of Spodoptera litura, the utility model also statistically analyzes the collection effect of the device and different attracting factors on the egg masses of Spodoptera litura. 12 treatment devices (including 3 repetitions) are placed between asparagus planting sheds, about 15 meters apart from each other, and the egg collection bucket is supported by a tripod, which is basically flush with the vents on both sides of the shed. First, a cylindrical paper tube with circular holes cut into it is placed in the egg collection bucket, and then an LED lamp is placed in the center of the cylindrical paper tube (the battery is placed on the ground). The egg masses are collected at 8 o'clock every morning for 3 consecutive days, and the types and quantities of various egg masses are counted, and they are bred indoors to count the hatching rate.

[0153] Table 3 Number of egg masses of Spodoptera litura

[0154]

[0155] Note: Different lowercase letters in the same column indicate significant differences (Duncan's new multiple range method, P<0.05).

[0156] The effects of different treatment groups in attracting Spodoptera litura eggs are shown in Table 3. The results show that the utility model egg collector and the matching attracting method have excellent egg mass collection capabilities:

[0157] (1) The results of treatments A to C showed that different light intensities had different effects on the egg mass collection capacity. When the light intensity was 2-6 watts, there was no significant difference in the egg mass collection capacity among different groups. However, when the light intensity was 4 watts, the egg mass collection capacity reached the highest level, and the amount of eggs collected was the highest.

[0158] (2) The results of treatments D to E show that the sweet and sour liquid has a good ability to attract insects. When water is used to replace the sweet and sour liquid, the attracting effect on adult insects is poor. This shows that the specific chemical components and odor characteristics contained in the sweet and sour liquid play a vital role in attracting insects. The sugar and acidic substances in the sweet and sour liquid may form unique chemical signals that can be keenly perceived and recognized by insects, thereby triggering their tendency to behave. Water lacks these specific chemical components and odor information, and cannot effectively activate the insect's sensory system and foraging instinct, resulting in a decrease in the attracting effect on adult insects.

[0159] (3) The results of treatments E to J showed that different attractants had different abilities to collect egg masses. When only sweet and sour liquid was used in combination with a single plant-based attractant or synergist, the attracting effect on adult insects was poor, indicating that only a single attractant was not attractive enough to adult insects. When a mixture of plant-based attractants and synergists was used, the attracting effect on adult insects was enhanced to a certain extent compared to a single attractant. This indicates that adding sweet and sour liquid and a suitable synergist has a certain synergistic effect on egg mass collection.

[0160] When plant-derived attractants and enhancers were evenly and spaced on the inner wall of the cylindrical tube, the results showed that compared with the mixture of plant-derived attractants + enhancers, the attracting effect on adult insects was improved. This may be because, on the one hand, the interval coating may cause the plant-derived attractants and enhancers to form higher concentrations in local areas, which is consistent with the concentration-dependent characteristics of chemical signal transmission. Higher concentrations of signal molecules are more easily perceived and responded to by adult insects, thereby enhancing the attraction to adult insects. The components in the mixture may dilute each other, reducing the concentration of the active ingredients and weakening the signal intensity, which in turn affects Attraction effect; on the other hand, in the mixture, the molecules of plant-derived attractants and synergists may compete with each other for binding sites with adults, and intermittent coating can reduce this competition, allowing each to play a more effective role, so that the synergist can more accurately enhance the odor characteristics of the plant-derived attractant, thereby improving the attracting effect on adults; at the same time, although plant-derived attractants and synergists can also play a certain synergistic role in the mixture, intermittent coating may create conditions that are more conducive to their synergistic effect. This specific arrangement may make it easier for adults to perceive the presence of attractants and synergists, thereby improving the attracting effect. Therefore, it is not necessary to simply mix attractants and synergists together to achieve the best effect. Instead, it is necessary to deeply explore and optimize their combination methods and coating strategies to achieve the highest attracting effect on adults, thereby maximizing the ability to collect egg masses. The reason is that the perception and behavioral response mechanisms of insects are very complex, and it is necessary to comprehensively consider the effects of multiple factors on them, and achieve the best attracting effect through fine regulation and optimization.

[0161] At the same time, in this embodiment, the volume ratio of the plant-derived attractant and the synergist was also screened, and the results showed that when the volume ratio of the plant-derived attractant (fresh juice of tender asparagus) and the synergist (cis-3-hexene acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L) was 1:1-3, it had a good ability to attract the adults of Spodoptera litura, and when the volume ratio of the two was 1:2, the ability to attract the adults reached the highest, which may be because different ratios will produce different effects. When the volume ratio of the two is appropriate, the synergist can fully enhance the effect of the plant-derived attractant, making the odor signal it releases stronger and more attractive; if the ratio of the synergist is too low, the enhancement effect may not be fully exerted; and if the ratio is too high, it may cover up or interfere with the original attraction of the plant-derived attractant to insects to a certain extent, or produce some unexpected effects. Therefore, in this embodiment, the volume ratio of the plant-derived attractant and the synergist is selected to be 1:1-3, and preferably 1:2.

[0162] (4) The results of treatments I to L showed that compared with the attracting ability and egg mass collection ability of treatments I and J, the egg mass collection ability of the egg collector was significantly enhanced after the addition of the insect attracting light source, which indicates that the combination of suitable insect attracting light source + sweet and sour liquid (flavor source bucket) + attractant (plant-derived attractant (fresh juice of tender asparagus) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L)) has the strongest attracting activity, indicating that the combination of suitable attractants (plant-derived attractant + synergist) has a significant synergistic effect on the egg mass collection ability of the insect attracting light source. At the same time, the attracting effect of treatment A was second, indicating that the insect attracting light source has a significant role in attracting adults and laying eggs.

[0163] At the same time, the analysis of the hatching rate of the eggs of Spodoptera litura showed that the indoor hatching rate of the eggs collected in the 13 treatments was 62-91%, which was in line with the expected hatching rate of the eggs. In treatment L, one device could collect more than 450 effective eggs (insects) per day, which was sufficient for monitoring the resistance of Spodoptera litura.

[0164] Example 5 The importance of different synergists in the utility model insect egg collector for collecting different insect egg masses

[0165] According to the effects of the above-mentioned different attractants on the egg mass collection of different insects, it is found that for different insects, the use of different synergists also has completely different effects on their egg mass collection ability.

[0166] For fall armyworm, when the attractant coated on the inner wall of the cylindrical tube is: plant-based attractant (fresh juice of corn plants) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: nonanal (mass ratio) = 1:0.5:2, 10 mg / L), the volume ratio of the plant-based attractant to the synergist is 1:2, and its ability to attract fall armyworm and collect egg masses is the highest, with more than 300 effective eggs collected per day; and when 3-butenyl isothiocyanate is used to replace nonanal, the attractant's ability to attract fall armyworm is significantly reduced, and the final egg mass collection ability is also significantly reduced, with only about 110 effective eggs collected per day. This may be because fall armyworms have different sensitivities and tendencies to different chemicals. Nonanal or chemical substances with similar structures may have specific action sites or signal transduction pathways in the olfactory perception mechanism of fall armyworm, and can attract fall armyworm more effectively, while 3-butenyl isothiocyanate may not be able to bind to the corresponding receptors of fall armyworm or activate the corresponding neural signals well, resulting in a decrease in its ability to attract it. It is also possible that the odor characteristics of 3-butenyl isothiocyanate do not match the odors related to the natural food source or habitat of fall armyworm, making it difficult to identify it as favorable information, thereby reducing its attraction to fall armyworm, and further significantly reducing the final egg mass collection ability. And when the ratio of each component in the synergist changes, the attractant's ability to attract fall armyworm also decreases. When the mass ratio of 3-hexenyl acetate: trans-2-hexenal: nonanal is in the range of 1-3:0.5-1.5:2-6, it can have a good ability to attract fall armyworm or other insects of the same type.

[0167] As for the beet armyworm and the armyworm, when the attractant coated on the inner wall of the cylindrical tube at intervals is: plant-based attractant (fresh juice of young asparagus) + synergist (cis-3-hexenyl acetate: trans-2-hexenal: 3-butenyl isothiocyanate (mass ratio) = 20:5:1, 10 mg / L), among which, when the volume ratio of the plant-based attractant and the synergist is 1:2, its ability to attract and collect egg masses for the beet armyworm and the armyworm reaches the highest, and the effective number of eggs collected per day is more than 500 and 450, respectively; and when nonanal is used to replace 3-butenyl isothiocyanate, the attractant's ability to attract the beet armyworm and the armyworm is significantly reduced, and finally the egg mass collection ability is also significantly reduced, and the effective number of eggs collected per day is only about 150. This may be because nonanal and 3-butenyl isothiocyanate have different action sites or signal transduction pathways in the olfactory perception mechanism of beet armyworm and Spodoptera litura. 3-Butenyl isothiocyanate and compounds with similar structural properties may more effectively bind to the corresponding receptors of beet armyworm and Spodoptera litura or activate the corresponding neural signals, thereby enhancing their attraction. However, nonanal may not bind to these receptors or activate the corresponding signals well, resulting in a decrease in the ability to attract beet armyworm and Spodoptera litura. When the ratio of each component in the synergist changes, the ability of the attractant to attract fall armyworm also decreases. When the mass ratio of 3-hexenyl acetate: trans-2-hexenal: 3-butenyl isothiocyanate is within the range of 18-22: 2-8: 0.5-2, it can have a good ability to attract beet armyworm, Spodoptera litura or other insects of the same type.

[0168] Although the present invention has been disclosed as above in terms of preferred embodiments, it is not intended to limit the present invention. Anyone familiar with the technology may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be based on the definition of the claims.

Claims

1. An insect egg collector, characterized in that: It comprises an insect egg collection barrel and a flavor source barrel, wherein the insect egg collection barrel and the flavor source barrel are combined into a detachable integrated structure for completing automatic insect attraction and automatic collection of insect eggs; The insect egg collection bucket is used to automatically attract insects, collect insect egg masses, and allow the insects to move freely; The odor source barrel is used for automatically attracting insects, thereby improving the insect trapping capability in the insect egg collection barrel.

2. The insect egg collector according to claim 1, characterized in that: The insect egg collection bucket is detachably fixed above the flavor source bucket, and the centers of the insect egg collection bucket and the flavor source bucket are on the same connecting line, which is perpendicular to the horizontal plane.

3. The insect egg collector according to claim 2, characterized in that: The insect egg collecting bucket comprises a cover body and an egg laying bucket, wherein a cylindrical tube is detachably fixed in the center of the egg laying bucket; the cover body and the egg laying bucket are combined into a detachable integrated structure, and the cover body is detachably fixed above the egg laying bucket to form a relatively closed space in cooperation with the egg laying bucket to prevent insects from escaping.

4. The insect egg collector according to claim 3, characterized in that: The wall of the egg-laying barrel is provided with long strip insect entrance holes, which are equidistant and evenly distributed, and are used to provide an entry channel for insects, making it convenient for insects to enter the egg-laying barrel.

5. The insect egg collector according to claim 4, characterized in that: The bottom of the egg-laying barrel is provided with 10-30 small holes for emitting odors to attract insects, which is beneficial to the circulation of air and the discharge of excess water.

6. The insect egg collector according to claim 5, characterized in that: The small holes are evenly arranged in the area between the egg laying bucket and the cylindrical tube.

7. The insect egg collector according to claim 6, characterized in that: The insect egg collector also includes an insect attracting light source, which is detachably installed in the center of the cylindrical tube; the cylindrical tube divides the egg-laying barrel into an insect attracting light source area, an insect activity area, and an insect egg-laying area; after the insects are attracted to the insect activity area, they lay eggs in the insect egg-laying area.

8. The insect egg collector according to claim 7, characterized in that: The cylindrical tube side surface is provided with 8 rows of circular holes, which are evenly and equidistantly arranged up and down, so as to facilitate the overflow of light from the insect attracting light source area and prevent insects from entering, so that insects gather in large numbers in the insect activity area, which is convenient for the collection of egg masses.

9. The insect egg collector according to claim 8, characterized in that: The long strip insect inlet hole is aligned with the circular hole on the side surface of the cylindrical tube, which is convenient for light overflow and insect trapping.

10. The insect egg collector according to claim 9, characterized in that: The centers of the egg-laying barrel, the cylindrical barrel and the flavor source barrel are located on the same connecting line, which is perpendicular to the horizontal plane; the flavor source barrel contains an attractant.